diff varscan/varscan_somatic.pl @ 4:572397bbe057 draft default tip

Added Xmx10G setting to both varscan_somatic wrappers

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/varscan/varscan_somatic.pl	Wed Mar 26 05:24:22 2014 -0400
@@ -0,0 +1,173 @@
+#!/usr/bin/perl
+
+
+use strict;
+use Cwd;
+
+die qq(
+Bad numbr of inputs
+
+) if(!@ARGV);
+
+my $options ="";
+my $normal="";
+my $command="";
+my $tumor="";
+my $tumorbam = "";
+my $output="";
+my $snp="";
+my $indel="";
+my $working_dir = cwd();
+my $log = '';
+
+foreach my $input (@ARGV) 
+{
+	my @tmp = split "::", $input;
+	if($tmp[0] eq "COMMAND") 
+	{
+		$command = $tmp[1];
+	} 
+	elsif($tmp[0] eq "NORMAL") 
+	{
+		$normal = $tmp[1];
+	} 
+	elsif($tmp[0] eq "TUMOR") 
+	{
+		$tumor = $tmp[1];
+	}
+	elsif($tmp[0] eq "TUMORBAM")
+	{
+		$tumorbam = $tmp[1];
+	}
+	elsif($tmp[0] eq "OPTION") 
+	{
+		my @p = split(/\s+/,$tmp[1]);
+		if ($p[0] =~ m/validation|strand-filter|output-vcf/ && $p[1] == 0) {
+			next;
+		}
+		$options = "$options ${tmp[1]}";
+	}
+	elsif($tmp[0] eq "OUTPUT") 
+	{
+		$output = $tmp[1];
+	}
+	elsif($tmp[0] eq "SNP") 
+	{
+		$snp = $tmp[1];
+	}
+	elsif($tmp[0] eq "INDEL") 
+	{
+		$indel = $tmp[1];
+	}
+
+	elsif($tmp[0] eq "LOG")
+        {
+                $log = $tmp[1];
+        }
+	
+	else 
+	{
+		die("Unknown Input: $input\n");
+	}
+}
+
+## VCF OUTPUT 
+if ($output ne '') {
+	system ("$command $normal $tumor $options --output-snp $working_dir/out.snp --output-indel $working_dir/out.indel 2>$log");
+	my $indels = "$working_dir/out.indel.vcf";
+	my $snps = "$working_dir/out.snp.vcf";
+	
+	system("grep -v '^\#' $indels | grep -v '^chrom position' >> $snps");
+	my @chr_ord = chromosome_order($tumorbam);
+
+	vs2vcf($snps, $output,\@chr_ord);
+}
+## SNP/INDEL OUTPUT
+else {
+	system  ("$command $normal $tumor $options --output-snp $snp --output-indel $indel 2>$log");
+}
+
+sub vs2vcf 
+{
+
+	#
+	# G l o b a l     v a r i a b l e s 
+	#
+	my $version = '0.1';
+
+	#
+	# Read in file
+	#
+	my $input = shift;
+	my $output = shift;
+	my $chr_ord = shift;
+	open(IN, $input) or die "Can't open $input': $!\n";
+	open(OUT, ">$output") or die "Can't create $output': $!\n";
+	my %output;
+
+	while ( <IN> )
+	{
+		if ( /^#/ )
+		{
+			print OUT;
+			next;
+		}
+		chomp;
+		my $line = $_;
+
+		my @flds = split ( "\t", $line );
+		my $ref = $flds[3];
+		my $alt = $flds[4];
+		#
+		# Deletion of bases
+		#
+		if ( $alt =~ /^\-/ )
+		{
+			($flds[3], $flds[4]) = ($ref.substr($alt,1), $ref);
+		}
+
+		#
+		# Insertion of bases
+		#
+		if ( $alt =~ /^\+/ )
+		{
+			$flds[4] = $ref.substr($alt,1);
+		}
+		## insert dot for reference positions.
+		if ($flds[4] eq '') {
+			$flds[4] = '.';
+		}
+		print OUT join( "\t", @flds),"\n" unless defined $chr_ord;
+		$output{$flds[0]}{$flds[1]} = join( "\t", @flds)."\n" if defined $chr_ord;
+	}
+	close(IN);
+	# if chromosome order given return in sorted order
+	if(defined $chr_ord) 
+	{
+		for my $chrom (@{ $chr_ord }) 
+		{
+			for my $pos (sort {$a<=>$b} keys %{ $output{$chrom} }) 
+			{
+				print OUT $output{$chrom}{$pos};
+			}
+		}
+	}
+	close(OUT);
+}
+
+
+sub chromosome_order 
+{
+	my $input = shift;
+	# calculate flagstats
+	my $COMM = "samtools view -H $input | grep '^\@SQ'";
+	my @SQ = `$COMM`;
+	chomp @SQ;
+	for(my $i = 0; $i <= $#SQ; $i++) 
+	{
+		$SQ[$i] =~ s/^\@SQ\tSN:(.*?)\tLN:\d+$/$1/;
+	} 
+	return(@SQ);
+}
+
+