diff src/Maaslin.R @ 0:e0b5980139d9

maaslin
author george-weingart
date Tue, 13 May 2014 22:00:40 -0400
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/src/Maaslin.R	Tue May 13 22:00:40 2014 -0400
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+#!/usr/bin/env Rscript
+#####################################################################################
+#Copyright (C) <2012>
+#
+#Permission is hereby granted, free of charge, to any person obtaining a copy of
+#this software and associated documentation files (the "Software"), to deal in the
+#Software without restriction, including without limitation the rights to use, copy,
+#modify, merge, publish, distribute, sublicense, and/or sell copies of the Software,
+#and to permit persons to whom the Software is furnished to do so, subject to
+#the following conditions:
+#
+#The above copyright notice and this permission notice shall be included in all copies
+#or substantial portions of the Software.
+#
+#THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED,
+#INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A
+#PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT
+#HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION
+#OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE
+#SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.
+#
+# This file is a component of the MaAsLin (Multivariate Associations Using Linear Models), 
+# authored by the Huttenhower lab at the Harvard School of Public Health
+# (contact Timothy Tickle, ttickle@hsph.harvard.edu).
+#####################################################################################
+
+inlinedocs <- function(
+##author<< Curtis Huttenhower <chuttenh@hsph.harvard.edu> and Timothy Tickle <ttickle@hsph.harvard.edu>
+##description<< Main driver script. Should be called to perform MaAsLin Analysis.
+) { return( pArgs ) }
+
+
+### Install packages if not already installed
+vDepLibrary = c("agricolae", "gam", "gamlss", "gbm", "glmnet", "inlinedocs", "logging", "MASS", "nlme", "optparse", "outliers", "penalized", "pscl", "robustbase", "testthat")
+for(sDepLibrary in vDepLibrary)
+{
+  if(! require(sDepLibrary, character.only=TRUE) )
+  {
+    install.packages(pkgs=sDepLibrary, repos="http://cran.us.r-project.org")
+  }
+}
+
+### Logging class
+suppressMessages(library( logging, warn.conflicts=FALSE, quietly=TRUE, verbose=FALSE))
+### Class for commandline argument processing
+suppressMessages(library( optparse, warn.conflicts=FALSE, quietly=TRUE, verbose=FALSE))
+
+
+### Create command line argument parser
+pArgs <- OptionParser( usage = "%prog [options] <output.txt> <data.tsv>" )
+
+# Input files for MaAsLin
+## Data configuration file
+pArgs <- add_option( pArgs, c("-i", "--input_config"), type="character", action="store", dest="strInputConfig", metavar="data.read.config", help="Optional configuration file describing data input format.")
+## Data manipulation/normalization file
+pArgs <- add_option( pArgs, c("-I", "--input_process"), type="character", action="store", dest="strInputR", metavar="data.R", help="Optional configuration script normalizing or processing data.")
+
+# Settings for MaAsLin
+## Maximum false discovery rate
+pArgs <- add_option( pArgs, c("-d", "--fdr"), type="double", action="store", dest="dSignificanceLevel", default=0.25, metavar="significance", help="The threshold to use for significance for the generated q-values (BH FDR). Anything equal to or lower than this is significant.  [Default %default]")
+## Minimum feature relative abundance filtering
+pArgs <- add_option( pArgs, c("-r", "--minRelativeAbundance"), type="double", action="store", dest="dMinAbd", default=0.0001, metavar="minRelativeAbundance", help="The minimum relative abundance allowed in the data. Values below this are removed and imputed as the median of the sample data.  [Default %default]")
+## Minimum feature prevalence filtering
+pArgs <- add_option( pArgs, c("-p", "--minPrevalence"), type="double", action="store", dest="dMinSamp", default=0.1, metavar="minPrevalence", help="The minimum percentage of samples a feature can have abundance in before being removed. Also is the minimum percentage of samples a metadata can have that are not NA before being removed.  [Default %default]")
+## Fence for outlier, if not set Grubbs test is used
+pArgs <- add_option( pArgs, c("-o", "--outlierFence"), type="double", action="store", dest="dOutlierFence", default=0, metavar="outlierFence", help="Outliers are defined as this number times the interquartile range added/subtracted from the 3rd/1st quartiles respectively. If set to 0 (default), outliers are defined by the Grubbs test.  [Default %default]")
+## Significance for Grubbs test
+pArgs <- add_option(pArgs, c("-G","--grubbsSig"), type="double", action="store", dest="dPOutlier", default=0.05, metavar="grubbsAlpha", help="This is the significance cuttoff used to indicate an outlier or not. The closer to zero, the more significant an outlier must be to be removed.  [Default %default]")
+## Fixed (not random) covariates
+pArgs <- add_option( pArgs, c("-R","--random"), type="character", action="store", dest="strRandomCovariates", default=NULL, metavar="fixed", help="These metadata will be treated as random covariates. Comma delimited data feature names. These features must be listed in the read.config file. Example '-R RandomMetadata1,RandomMetadata2'.  [Default %default]")
+## Change the type of correction fo rmultiple corrections
+pArgs <- add_option( pArgs, c("-T","--testingCorrection"), type="character", action="store", dest="strMultTestCorrection", default="BH", metavar="multipleTestingCorrection", help="This indicates which multiple hypothesis testing method will be used, available are holm, hochberg, hommel, bonferroni, BH, BY.  [Default %default]")
+## Use a zero inflated model of the inference method indicate in -m
+pArgs <- add_option( pArgs, c("-z","--doZeroInfated"), type="logical", action="store_true", default = FALSE, dest="fZeroInflated", metavar="fZeroInflated", help="If true, the zero inflated version of the inference model indicated in -m is used. For instance if using lm, zero-inflated regression on a gaussian distribution is used.  [Default %default].")
+
+# Arguments used in validation of MaAsLin
+## Model selection (enumerate) c("none","boost","penalized","forward","backward")
+pArgs <- add_option( pArgs, c("-s", "--selection"), type="character", action="store", dest="strModelSelection", default="boost", metavar="model_selection", help="Indicates which of the variable selection techniques to use.  [Default %default]")
+## Argument indicating which method should be ran (enumerate) c("univariate","lm","neg_binomial","quasi")
+pArgs <- add_option( pArgs, c("-m", "--method"), type="character", action="store", dest="strMethod", default="lm", metavar="analysis_method", help="Indicates which of the statistical inference methods to run.  [Default %default]")
+## Argument indicating which link function is used c("none","asinsqrt")
+pArgs <- add_option( pArgs, c("-l", "--link"), type="character", action="store", dest="strTransform", default="asinsqrt", metavar="transform_method", help="Indicates which link or transformation to use with a glm, if glm is not selected this argument will be set to none.  [Default %default]")
+pArgs <- add_option( pArgs, c("-Q","--NoQC"), type="logical", action="store_true", default=FALSE, dest="fNoQC", metavar="Do_Not_Run_QC", help="Indicates if the quality control will be ran on the metadata/data. Default is true.  [Default %default]")
+
+# Arguments to suppress MaAsLin actions on certain data
+## Do not perform model selection on the following data
+pArgs <- add_option( pArgs, c("-F","--forced"), type="character", action="store", dest="strForcedPredictors", default=NULL, metavar="forced_predictors", help="Metadata features that will be forced into the model seperated by commas. These features must be listed in the read.config file. Example '-F Metadata2,Metadata6,Metadata10'.  [Default %default]")
+## Do not impute the following
+pArgs <- add_option( pArgs, c("-n","--noImpute"), type="character", action="store", dest="strNoImpute", default=NULL, metavar="no_impute", help="These data will not be imputed. Comma delimited data feature names. Example '-n Feature1,Feature4,Feature6'.  [Default %default]")
+
+#Miscellaneouse arguments
+### Argument to control logging (enumerate)
+strDefaultLogging = "DEBUG"
+pArgs <- add_option( pArgs, c("-v", "--verbosity"), type="character", action="store", dest="strVerbosity", default=strDefaultLogging, metavar="verbosity", help="Logging verbosity  [Default %default]")
+### Run maaslin without creating a log file
+pArgs <- add_option( pArgs, c("-O","--omitLogFile"), type="logical", action="store_true", default=FALSE, dest="fOmitLogFile", metavar="omitlogfile",help="Including this flag will stop the creation of the output log file.  [Default %default]")
+### Argument for inverting background to black
+pArgs <- add_option( pArgs, c("-t", "--invert"), type="logical", action="store_true", dest="fInvert", default=FALSE, metavar="invert", help="When given, flag indicates to invert the background of figures to black.  [Default %default]")
+### Selection Frequency
+pArgs <- add_option( pArgs, c("-f","--selectionFrequency"), type="double", action="store", dest="dSelectionFrequency", default=NA, metavar="selectionFrequency", help="Selection Frequency for boosting (max 1 will remove almost everything). Interpreted as requiring boosting to select metadata 100% percent of the time (or less if given a number that is less). Value should be between 1 (100%) and 0 (0%), NA (default is determined by data size).")
+### All v All
+pArgs <- add_option( pArgs, c("-a","--allvall"), type="logical", action="store_true", dest="fAllvAll", default=FALSE, metavar="compare_all", help="When given, the flag indicates that each fixed covariate that is not indicated as Forced is compared once at a time per data feature (bug). Made to be used with the -F option to specify one part of the model while allowing the other to cycle through a group of covariates. Does not affect Random covariates, which are always included when specified.  [Default %default]")
+pArgs <- add_option( pArgs, c("-N","--PlotNA"), type="logical", action="store_true", default=FALSE, dest="fPlotNA", metavar="plotNAs",help="Plot data that was originally NA, by default they are not plotted.  [Default %default]")
+### Alternative methodology settings
+pArgs <- add_option( pArgs, c("-A","--pAlpha"), type="double", action="store", dest="dPenalizedAlpha", default=0.95, metavar="PenalizedAlpha",help="The alpha for penalization (1.0=L1 regularization, LASSO; 0.0=L2 regularization, ridge regression.  [Default %default]")
+### Pass an alternative library dir
+pArgs <- add_option( pArgs, c("-L", "--libdir"), action="store", dest="sAlternativeLibraryLocation", default=file.path( "","usr","share","biobakery" ), metavar="AlternativeLibraryDirectory", help="An alternative location to find the lib directory. This dir and children will be searched for the first maaslin/src/lib dir.")
+
+### Misc biplot arguments
+pArgs <- add_option( pArgs, c("-M","--BiplotMetadataScale"), type="double", action="store", dest="dBiplotMetadataScale", default=1, metavar="scaleForMetadata", help="A real number used to scale the metadata labels on the biplot (otherwise a default will be selected from the data).  [Default %default]")
+pArgs <- add_option( pArgs, c("-C", "--BiplotColor"), type="character", action="store", dest="strBiplotColor", default=NULL, metavar="BiplotColorCovariate", help="A continuous metadata that will be used to color samples in the biplot ordination plot (otherwise a default will be selected from the data). Example Age  [Default %default]")
+pArgs <- add_option( pArgs, c("-S", "--BiplotShapeBy"), type="character", action="store", dest="strBiplotShapeBy", default=NULL, metavar="BiplotShapeCovariate", help="A discontinuous metadata that will be used to indicate shapes of samples in the Biplot ordination plot (otherwise a default will be selected from the data). Example Sex  [Default %default]")
+pArgs <- add_option( pArgs, c("-P", "--BiplotPlotFeatures"), type="character", action="store", dest="strBiplotPlotFeatures", default=NULL, metavar="BiplotFeaturesToPlot", help="Metadata and data features to plot (otherwise a default will be selected from the data). Comma Delimited.")
+pArgs <- add_option( pArgs, c("-D", "--BiplotRotateMetadata"), type="character", action="store", dest="sRotateByMetadata", default=NULL, metavar="BiplotRotateMetadata", help="Metadata to use to rotate the biplot. Format 'Metadata,value'. 'Age,0.5' .  [Default %default]")
+pArgs <- add_option( pArgs, c("-B", "--BiplotShapes"), type="character", action="store", dest="sShapes", default=NULL, metavar="BiplotShapes", help="Specify shapes specifically for metadata or metadata values.  [Default %default]")
+pArgs <- add_option( pArgs, c("-b", "--BugCount"), type="integer", action="store", dest="iNumberBugs", default=3, metavar="PlottedBugCount", help="The number of bugs automatically selected from the data to plot.  [Default %default]")
+pArgs <- add_option( pArgs, c("-E", "--MetadataCount"), type="integer", action="store", dest="iNumberMetadata", default=NULL, metavar="PlottedMetadataCount", help="The number of metadata automatically selected from the data to plot.  [Default all significant metadata and minimum is 1]")
+
+#pArgs <- add_option( pArgs, c("-c","--MFAFeatureCount"), type="integer", action="store", dest="iMFAMaxFeatures", default=3, metavar="maxMFAFeature", help="Number of features or number of bugs to plot (default=3; 3 metadata and 3 data).")
+
+main <- function(
+### The main function manages the following:
+### 1. Optparse arguments are checked
+### 2. A logger is created if requested in the optional arguments
+### 3. The custom R script is sourced. This is the input *.R script named
+### the same as the input *.pcl file. This script contains custom formating
+### of data and function calls to the MFA visualization.
+### 4. Matrices are written to the project folder as they are read in seperately as metadata and data and merged together.
+### 5. Data is cleaned with custom filtering if supplied in the *.R script.
+### 6. Transformations occur if indicated by the optional arguments
+### 7. Standard quality control is performed on data
+### 8. Cleaned metadata and data are written to output project for documentation.
+### 9. A regularization method is ran (boosting by default).
+### 10. An analysis method is performed on the model (optionally boosted model).
+### 11. Data is summarized and PDFs are created for significant associations
+### (those whose q-values {BH FDR correction} are <= the threshold given in the optional arguments.
+pArgs
+### Parsed commandline arguments
+){
+  lsArgs <- parse_args( pArgs, positional_arguments = TRUE )
+  #logdebug("lsArgs", c_logrMaaslin)
+  #logdebug(paste(lsArgs,sep=" "), c_logrMaaslin)
+
+  # Parse parameters
+  lsForcedParameters = NULL
+  if(!is.null(lsArgs$options$strForcedPredictors))
+  {
+    lsForcedParameters  = unlist(strsplit(lsArgs$options$strForcedPredictors,","))
+  }
+  xNoImpute = NULL
+  if(!is.null(lsArgs$options$strNoImpute))
+  {
+    xNoImpute = unlist(strsplit(lsArgs$options$strNoImpute,"[,]"))
+  }
+  lsRandomCovariates = NULL
+  if(!is.null(lsArgs$options$strRandomCovariates))
+  {
+    lsRandomCovariates = unlist(strsplit(lsArgs$options$strRandomCovariates,"[,]"))
+  }
+  lsFeaturesToPlot = NULL
+  if(!is.null(lsArgs$options$strBiplotPlotFeatures))
+  {
+    lsFeaturesToPlot = unlist(strsplit(lsArgs$options$strBiplotPlotFeatures,"[,]"))
+  }
+
+  #If logging is not an allowable value, inform user and set to INFO
+  if(length(intersect(names(loglevels), c(lsArgs$options$strVerbosity))) == 0)
+  {
+    print(paste("Maaslin::Error. Did not understand the value given for logging, please use any of the following: DEBUG,INFO,WARN,ERROR."))
+    print(paste("Maaslin::Warning. Setting logging value to \"",strDefaultLogging,"\"."))
+  }
+
+  # Do not allow  mixed effect models and zero inflated models, don't have implemented
+  if(lsArgs$options$fZeroInflated && !is.null(lsArgs$options$strRandomCovariates))
+  {
+    stop("MaAsLin Error:: The combination of zero inflated models and mixed effects models are not supported.")
+  }
+
+  ### Create logger
+  c_logrMaaslin <- getLogger( "maaslin" )
+  addHandler( writeToConsole, c_logrMaaslin )
+  setLevel( lsArgs$options$strVerbosity, c_logrMaaslin )
+
+  #Get positional arguments
+  if( length( lsArgs$args ) != 2 ) { stop( print_help( pArgs ) ) }
+  ### Output file name
+  strOutputTXT <- lsArgs$args[1]
+  ### Input TSV data file
+  strInputTSV <- lsArgs$args[2]
+
+  # Get analysis method options
+  # includes data transformations, model selection/regularization, regression models/links
+  lsArgs$options$strModelSelection = tolower(lsArgs$options$strModelSelection)
+  if(!lsArgs$options$strModelSelection %in% c("none","boost","penalized","forward","backward"))
+  {
+    logerror(paste("Received an invalid value for the selection argument, received '",lsArgs$options$strModelSelection,"'"), c_logrMaaslin)
+    stop( print_help( pArgs ) )
+  }
+  lsArgs$options$strMethod = tolower(lsArgs$options$strMethod)
+  if(!lsArgs$options$strMethod %in% c("univariate","lm","neg_binomial","quasi"))
+  {
+    logerror(paste("Received an invalid value for the method argument, received '",lsArgs$options$strMethod,"'"), c_logrMaaslin)
+    stop( print_help( pArgs ) )
+  }
+  lsArgs$options$strTransform = tolower(lsArgs$options$strTransform)
+  if(!lsArgs$options$strTransform %in% c("none","asinsqrt"))
+  {
+    logerror(paste("Received an invalid value for the transform/link argument, received '",lsArgs$options$strTransform,"'"), c_logrMaaslin)
+    stop( print_help( pArgs ) )
+  }
+
+  if(!lsArgs$options$strMultTestCorrection %in% c("holm", "hochberg", "hommel", "bonferroni", "BH", "BY"))
+  {
+    logerror(paste("Received an invalid value for the multiple testing correction argument, received '",lsArgs$options$strMultTestCorrection,"'"), c_logrMaaslin)
+    stop( print_help( pArgs ) )
+  }
+
+  ### Necessary local import files
+  ### Check to make sure the lib is in the expected place (where the script is)
+  ### if not, then try the alternative lib location
+  ### This will happen if, for instance the script is linked or
+  ### on the path.
+  # Get the first choice relative path
+  initial.options <- commandArgs(trailingOnly = FALSE)
+  script.name <- sub("--file=", "", initial.options[grep("--file=", initial.options)])
+  strDir = file.path( dirname( script.name ), "lib" )
+  # If this does not have the lib file then go for the alt lib
+  if( !file.exists(strDir) )
+  {
+    lsPotentialListLocations = dir( path = lsArgs$options$sAlternativeLibraryLocation, pattern = "lib", recursive = TRUE, include.dirs = TRUE)
+    if( length( lsPotentialListLocations ) > 0 )
+    {
+      sLibraryPath = file.path( "maaslin","src","lib" )
+      iLibraryPathLength = nchar( sLibraryPath )
+      for( strSearchDir in lsPotentialListLocations )
+      {
+        # Looking for the path where the end of the path is equal to the library path given earlier
+        # Also checks before hand to make sure the path is atleast as long as the library path so no errors occur
+        if ( substring( strSearchDir, 1 + nchar( strSearchDir ) - iLibraryPathLength ) == sLibraryPath )
+        {
+          strDir = file.path( lsArgs$options$sAlternativeLibraryLocation, strSearchDir )
+          break
+        }
+      }
+    }
+  }
+  
+  strSelf = basename( script.name )
+  for( strR in dir( strDir, pattern = "*.R$" ) )
+  {
+    if( strR == strSelf ) {next}
+    source( file.path( strDir, strR ) )
+  }
+
+  # Get analysis modules
+  afuncVariableAnalysis = funcGetAnalysisMethods(lsArgs$options$strModelSelection,lsArgs$options$strTransform,lsArgs$options$strMethod,lsArgs$options$fZeroInflated)
+
+  # Set up parameters for variable selection
+  lxParameters = list(dFreq=lsArgs$options$dSelectionFrequency, dPAlpha=lsArgs$options$dPenalizedAlpha)
+  if((lsArgs$options$strMethod == "lm")||(lsArgs$options$strMethod == "univariate"))
+  { lxParameters$sFamily = "gaussian"
+  } else if(lsArgs$options$strMethod == "neg_binomial"){ lxParameters$sFamily = "binomial"
+  } else if(lsArgs$options$strMethod == "quasi"){ lxParameters$sFamily = "poisson"}
+
+  #Indicate start
+  logdebug("Start MaAsLin", c_logrMaaslin)
+  #Log commandline arguments
+  logdebug("Commandline Arguments", c_logrMaaslin)
+  logdebug(lsArgs, c_logrMaaslin)
+
+  ### Output directory for the study based on the requested output file
+  outputDirectory = dirname(strOutputTXT)
+  ### Base name for the project based on the read.config name
+  strBase <- sub("\\.[^.]*$", "", basename(strInputTSV))
+
+  ### Sources in the custom script
+  ### If the custom script is not there then
+  ### defaults are used and no custom scripts are ran
+  funcSourceScript <- function(strFunctionPath)
+  {
+    #If is specified, set up the custom func clean variable
+    #If the custom script is null then return 
+    if(is.null(strFunctionPath)){return(NULL)}
+
+    #Check to make sure the file exists
+    if(file.exists(strFunctionPath))
+    {
+      #Read in the file
+      source(strFunctionPath)
+    } else {
+      #Handle when the file does not exist
+      stop(paste("MaAsLin Error: A custom data manipulation script was indicated but was not found at the file path: ",strFunctionPath,sep=""))
+    }
+  }
+
+  #Read file
+  inputFileData = funcReadMatrices(lsArgs$options$strInputConfig, strInputTSV, log=TRUE)
+  if(is.null(inputFileData[[c_strMatrixMetadata]])) { names(inputFileData)[1] <- c_strMatrixMetadata }
+  if(is.null(inputFileData[[c_strMatrixData]])) { names(inputFileData)[2] <- c_strMatrixData }
+
+  #Metadata and bug names
+  lsOriginalMetadataNames = names(inputFileData[[c_strMatrixMetadata]])
+  lsOriginalFeatureNames = names(inputFileData[[c_strMatrixData]])
+
+  #Dimensions of the datasets
+  liMetaData = dim(inputFileData[[c_strMatrixMetadata]])
+  liData = dim(inputFileData[[c_strMatrixData]])
+
+  #Merge data files together
+  frmeData = merge(inputFileData[[c_strMatrixMetadata]],inputFileData[[c_strMatrixData]],by.x=0,by.y=0)
+  #Reset rownames
+  row.names(frmeData) = frmeData[[1]]
+  frmeData = frmeData[-1]
+
+  #Write QC files only in certain modes of verbosity
+  # Read in and merge files
+  if( c_logrMaaslin$level <= loglevels["DEBUG"] ) {
+    # If the QC internal file does not exist, make
+    strQCDir = file.path(outputDirectory,"QC")
+    dir.create(strQCDir, showWarnings = FALSE)
+    # Write metadata matrix before merge
+    funcWriteMatrices(dataFrameList=list(Metadata = inputFileData[[c_strMatrixMetadata]]), saveFileList=c(file.path(strQCDir,"metadata.tsv")), configureFileName=c(file.path(strQCDir,"metadata.read.config")), acharDelimiter="\t")
+    # Write data matrix before merge
+    funcWriteMatrices(dataFrameList=list(Data = inputFileData[[c_strMatrixData]]), saveFileList=c(file.path(strQCDir,"data.tsv")), configureFileName=c(file.path(strQCDir,"data.read.config")), acharDelimiter="\t")
+    #Record the data as it has been read
+    funcWriteMatrices(dataFrameList=list(Merged = frmeData), saveFileList=c(file.path(strQCDir,"read-Merged.tsv")), configureFileName=c(file.path(strQCDir,"read-Merged.read.config")), acharDelimiter="\t")
+  }
+
+  #Data needed for the MaAsLin environment
+  #List of lists (one entry per file)
+  #Is contained by a container of itself
+  #lslsData = list()
+  #List
+  lsData = c()
+
+  #List of metadata indicies
+  aiMetadata = c(1:liMetaData[2])
+  lsData$aiMetadata = aiMetadata
+  #List of data indicies
+  aiData = c(1:liData[2])+liMetaData[2]
+  lsData$aiData = aiData
+  #Add a list to hold qc metrics and counts
+  lsData$lsQCCounts$aiDataInitial = aiData
+  lsData$lsQCCounts$aiMetadataInitial = aiMetadata
+
+  #Raw data
+  lsData$frmeRaw = frmeData
+
+  #Load script if it exists, stop on error
+  funcProcess <- NULL
+  if(!is.null(funcSourceScript(lsArgs$options$strInputR))){funcProcess <- get(c_strCustomProcessFunction)}
+
+  #Clean the data and update the current data list to the cleaned data list
+  funcTransformData = afuncVariableAnalysis[[c_iTransform]]
+  lsQCCounts = list(aiDataCleaned = c(), aiMetadataCleaned = c())
+  lsRet = list(frmeData=frmeData, aiData=aiData, aiMetadata=aiMetadata, lsQCCounts=lsQCCounts, liNaIndices=c())
+
+  viNotTransformedDataIndices = c()
+  if(!lsArgs$options$fNoQC)
+  {
+    c_logrMaaslin$info( "Running quality control." )
+    lsRet = funcClean( frmeData=frmeData, funcDataProcess=funcProcess, aiMetadata=aiMetadata, aiData=aiData, lsQCCounts=lsData$lsQCCounts, astrNoImpute=xNoImpute, dMinSamp = lsArgs$options$dMinSamp, dMinAbd = lsArgs$options$dMinAbd, dFence=lsArgs$options$dOutlierFence, funcTransform=funcTransformData, dPOutlier=lsArgs$options$dPOutlier)
+
+    viNotTransformedDataIndices = lsRet$viNotTransformedData
+
+    #If using a count based model make sure all are integer (QCing can add in numeric values during interpolation for example)
+    if(lsArgs$options$strMethod %in% c_vCountBasedModels)
+    {
+      c_logrMaaslin$info( "Assuring the data matrix is integer." )
+      for(iDataIndex in aiData)
+      {
+        lsRet$frmeData[ iDataIndex ] = round( lsRet$frmeData[ iDataIndex ] )
+      }
+    }
+  } else {
+    c_logrMaaslin$info( "Not running quality control, attempting transform." )
+    ### Need to do transform if the QC is not performed
+    iTransformed = 0
+    for(iDataIndex in aiData)
+    {
+      if( ! funcTransformIncreasesOutliers( lsRet$frmeData[iDataIndex], funcTransformData ) )
+      {
+        lsRet$frmeData[iDataIndex]=funcTransformData(lsRet$frmeData[iDataIndex])
+        iTransformed = iTransformed + 1
+      } else {
+        viNotTransformedDataIndices = c(viNotTransformedDataIndices, iDataIndex)
+      }
+    }
+    c_logrMaaslin$info(paste("Number of features transformed = ", iTransformed))
+  }
+
+  logdebug("lsRet", c_logrMaaslin)
+  logdebug(format(lsRet), c_logrMaaslin)
+  #Update the variables after cleaning
+  lsRet$frmeRaw = frmeData
+  lsRet$lsQCCounts$aiDataCleaned = lsRet$aiData
+  lsRet$lsQCCounts$aiMetadataCleaned = lsRet$aiMetadata
+
+  #Add List of metadata string names
+  astrMetadata = colnames(lsRet$frmeData)[lsRet$aiMetadata]
+  lsRet$astrMetadata = astrMetadata
+
+  # If plotting NA data reset the NA metadata indices to empty so they will not be excluded
+  if(lsArgs$options$fPlotNA)
+  {
+    lsRet$liNaIndices = list()
+  }
+
+  #Write QC files only in certain modes of verbosity
+  if( c_logrMaaslin$level <= loglevels["DEBUG"] ) {
+    #Record the data after cleaning
+    funcWriteMatrices(dataFrameList=list(Cleaned = lsRet$frmeData[union(lsRet$aiMetadata,lsRet$aiData)]), saveFileList=c(file.path(strQCDir,"read_cleaned.tsv")), configureFileName=c(file.path(strQCDir,"read_cleaned.read.config")), acharDelimiter="\t") }
+
+  #These variables will be used to count how many features get analysed
+  lsRet$lsQCCounts$iBoosts = 0
+  lsRet$lsQCCounts$iBoostErrors = 0
+  lsRet$lsQCCounts$iNoTerms = 0
+  lsRet$lsQCCounts$iLms = 0
+
+  #Indicate if the residuals plots should occur
+  fDoRPlot=TRUE
+  #Should not occur for univariates
+  if(lsArgs$options$strMethod %in% c("univariate")){ fDoRPlot=FALSE }
+
+  #Run analysis
+  alsRetBugs = funcBugs( frmeData=lsRet$frmeData, lsData=lsRet, aiMetadata=lsRet$aiMetadata, aiData=lsRet$aiData, aiNotTransformedData=viNotTransformedDataIndices, strData=strBase, dSig=lsArgs$options$dSignificanceLevel, fInvert=lsArgs$options$fInvert,
+        strDirOut=outputDirectory, funcReg=afuncVariableAnalysis[[c_iSelection]], funcTransform=funcTransformData, funcUnTransform=afuncVariableAnalysis[[c_iUnTransform]], lsNonPenalizedPredictors=lsForcedParameters,
+        funcAnalysis=afuncVariableAnalysis[[c_iAnalysis]], lsRandomCovariates=lsRandomCovariates, funcGetResults=afuncVariableAnalysis[[c_iResults]], fDoRPlot=fDoRPlot, fOmitLogFile=lsArgs$options$fOmitLogFile,
+        fAllvAll=lsArgs$options$fAllvAll, liNaIndices=lsRet$liNaIndices, lxParameters=lxParameters, strTestingCorrection=lsArgs$options$strMultTestCorrection, 
+        fIsUnivariate=afuncVariableAnalysis[[c_iIsUnivariate]], fZeroInflated=lsArgs$options$fZeroInflated )
+
+  #Write QC files only in certain modes of verbosity
+  if( c_logrMaaslin$level <= loglevels["DEBUG"] ) {
+	funcWriteQCReport(strProcessFileName=file.path(strQCDir,"ProcessQC.txt"), lsQCData=alsRetBugs$lsQCCounts, liDataDim=liData, liMetadataDim=liMetaData)
+
+    ### Write out the parameters used in the run
+    unlink(file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite("Parameters used in the MaAsLin run", file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Optional input read.config file=",lsArgs$options$strInputConfig), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Optional R file=",lsArgs$options$strInputR), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("FDR threshold for pdf generation=",lsArgs$options$dSignificanceLevel), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Minimum relative abundance=",lsArgs$options$dMinAbd), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Minimum percentage of samples with measurements=",lsArgs$options$dMinSamp), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("The fence used to define outliers with a quantile based analysis. If set to 0, the Grubbs test was used=",lsArgs$options$dOutlierFence), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Ignore if the Grubbs test was not used. The significance level used as a cut-off to define outliers=",lsArgs$options$dPOutlier), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("These covariates are treated as random covariates and not fixed covariates=",lsArgs$options$strRandomCovariates), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("The type of multiple testing correction used=",lsArgs$options$strMultTestCorrection), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Zero inflated inference models were turned on=",lsArgs$options$fZeroInflated), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Feature selection step=",lsArgs$options$strModelSelection), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Statistical inference step=",lsArgs$options$strMethod), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Numeric transform used=",lsArgs$options$strTransform), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Quality control was run=",!lsArgs$options$fNoQC), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("These covariates were forced into each model=",lsArgs$options$strForcedPredictors), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("These features' data were not changed by QC processes=",lsArgs$options$strNoImpute), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Output verbosity=",lsArgs$options$strVerbosity), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Log file was generated=",!lsArgs$options$fOmitLogFile), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Data plots were inverted=",lsArgs$options$fInvert), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Ignore unless boosting was used. The threshold for the rel.inf used to select features=",lsArgs$options$dSelectionFrequency), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("All verses all inference method was used=",lsArgs$options$fAllvAll), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Ignore unless penalized feature selection was used. Alpha to determine the type of penalty=",lsArgs$options$dPenalizedAlpha), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Biplot parameter, user defined metadata scale=",lsArgs$options$dBiplotMetadataScale), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Biplot parameter, user defined metadata used to color the plot=",lsArgs$options$strBiplotColor), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Biplot parameter, user defined metadata used to dictate the shapes of the plot markers=",lsArgs$options$strBiplotShapeBy), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Biplot parameter, user defined user requested features to plot=",lsArgs$options$strBiplotPlotFeatures), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Biplot parameter, user defined metadata used to rotate the plot ordination=",lsArgs$options$sRotateByMetadata), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Biplot parameter, user defined custom shapes for metadata=",lsArgs$options$sShapes), file.path(strQCDir,"Run_Parameters.txt"))
+    funcWrite(paste("Biplot parameter, user defined number of bugs to plot =",lsArgs$options$iNumberBugs), file.path(strQCDir,"Run_Parameters.txt"))
+  }
+
+  ### Write summary table
+  # Summarize output files based on a keyword and a significance threshold
+  # Look for less than or equal to the threshold (appropriate for p-value and q-value type measurements)
+  # DfSummary is sorted by the q.value when it is returned
+  dfSummary = funcSummarizeDirectory(astrOutputDirectory=outputDirectory,
+                       strBaseName=strBase,
+                       astrSummaryFileName=file.path(outputDirectory,paste(strBase,c_sSummaryFileSuffix, sep="")), 
+                       astrKeyword=c_strKeywordEvaluatedForInclusion, 
+                       afSignificanceLevel=lsArgs$options$dSignificanceLevel)
+
+  if( !is.null( dfSummary ) )
+  {
+    ### Start biplot
+    # Get metadata of interest and reduce to default size
+    lsSigMetadata = unique(dfSummary[[1]])
+    if( is.null( lsArgs$options$iNumberMetadata ) )
+    {
+      lsSigMetadata = lsSigMetadata[ 1:length( lsSigMetadata ) ]
+    } else {
+      lsSigMetadata = lsSigMetadata[ 1:min( length( lsSigMetadata ), max( lsArgs$options$iNumberMetadata, 1 ) ) ]
+    }
+
+    # Convert to indices (ordered numerically here)
+    liSigMetadata = which( colnames( lsRet$frmeData ) %in% lsSigMetadata )
+
+    # Get bugs of interest and reduce to default size
+    lsSigBugs = unique(dfSummary[[2]])
+
+    # Reduce the bugs to the right size
+    if(lsArgs$options$iNumberBugs < 1)
+    {
+      lsSigBugs = c()
+    } else if( is.null( lsArgs$options$iNumberBugs ) ) {
+      lsSigBugs = lsSigBugs[ 1 : length( lsSigBugs ) ]
+    } else {
+      lsSigBugs = lsSigBugs[ 1 : lsArgs$options$iNumberBugs ]
+    }
+
+    # Set color by and shape by features if not given
+    # Selects the continuous (for color) and factor (for shape) data with the most significant association
+    if(is.null(lsArgs$options$strBiplotColor)||is.null(lsArgs$options$strBiplotShapeBy))
+    {
+      for(sMetadata in lsSigMetadata)
+      {
+        if(is.factor(lsRet$frmeRaw[[sMetadata]]))
+        {
+          if(is.null(lsArgs$options$strBiplotShapeBy))
+          {
+            lsArgs$options$strBiplotShapeBy = sMetadata
+            if(!is.null(lsArgs$options$strBiplotColor))
+            {
+              break
+            }
+          }
+        }
+        if(is.numeric(lsRet$frmeRaw[[sMetadata]]))
+        {
+          if(is.null(lsArgs$options$strBiplotColor))
+          {
+            lsArgs$options$strBiplotColor = sMetadata
+            if(!is.null(lsArgs$options$strBiplotShapeBy))
+            {
+              break
+            }
+          }
+        }
+      }
+    }
+
+    #If a user defines a feature, make sure it is in the bugs/data indices
+    if(!is.null(lsFeaturesToPlot) || !is.null(lsArgs$options$strBiplotColor) || !is.null(lsArgs$options$strBiplotShapeBy))
+    {
+      lsCombinedFeaturesToPlot = unique(c(lsFeaturesToPlot,lsArgs$options$strBiplotColor,lsArgs$options$strBiplotShapeBy))
+      lsCombinedFeaturesToPlot = lsCombinedFeaturesToPlot[!is.null(lsCombinedFeaturesToPlot)]
+
+      # If bugs to plot were given then do not use the significant bugs from the MaAsLin output which is default
+      if(!is.null(lsFeaturesToPlot))
+      {
+        lsSigBugs = c()
+        liSigMetadata = c()
+      }
+      liSigMetadata = unique(c(liSigMetadata,which(colnames(lsRet$frmeData) %in% setdiff(lsCombinedFeaturesToPlot, lsOriginalFeatureNames))))
+      lsSigBugs = unique(c(lsSigBugs, intersect(lsCombinedFeaturesToPlot, lsOriginalFeatureNames)))
+    }
+
+    # Convert bug names and metadata names to comma delimited strings
+    vsBugs = paste(lsSigBugs,sep=",",collapse=",")
+    vsMetadata = paste(colnames(lsRet$frmeData)[liSigMetadata],sep=",",collapse=",")
+    vsMetadataByLevel = c()
+
+    # Possibly remove the NA levels depending on the preferences
+    vsRemoveNA = c(NA, "NA", "na", "Na", "nA")
+    if(!lsArgs$options$fPlotNA){ vsRemoveNA = c() }
+    for(aiMetadataIndex in liSigMetadata)
+    {
+      lxCurMetadata = lsRet$frmeData[[aiMetadataIndex]]
+      sCurName = names(lsRet$frmeData[aiMetadataIndex])
+      if(is.factor(lxCurMetadata))
+      {
+        vsMetadataByLevel = c(vsMetadataByLevel,paste(sCurName, setdiff( levels(lxCurMetadata), vsRemoveNA),sep="_"))
+      } else {
+        vsMetadataByLevel = c(vsMetadataByLevel,sCurName)
+      }
+    }
+
+    # If NAs should not be plotted, make them the background color
+    # Unless explicitly asked to be plotted
+    sPlotNAColor = "white"
+    if(lsArgs$options$fInvert){sPlotNAColor = "black"}
+    if(lsArgs$options$fPlotNA){sPlotNAColor = "grey"}
+    sLastMetadata = lsOriginalMetadataNames[max(which(lsOriginalMetadataNames %in% names(lsRet$frmeData)))]
+
+    # Plot biplot
+    logdebug("PlotBiplot:Started")
+    funcDoBiplot(
+      sBugs = vsBugs,
+      sMetadata = vsMetadataByLevel,
+      sColorBy = lsArgs$options$strBiplotColor,
+      sPlotNAColor = sPlotNAColor,
+      sShapeBy = lsArgs$options$strBiplotShapeBy,
+      sShapes = lsArgs$options$sShapes,
+      sDefaultMarker = "16",
+      sRotateByMetadata = lsArgs$options$sRotateByMetadata,
+      dResizeArrow = lsArgs$options$dBiplotMetadataScale,
+      sInputFileName = lsRet$frmeRaw,
+      sLastMetadata = sLastMetadata,
+      sOutputFileName = file.path(outputDirectory,paste(strBase,"-biplot.pdf",sep="")))
+    logdebug("PlotBiplot:Stopped")
+  }
+}
+
+# This is the equivalent of __name__ == "__main__" in Python.
+# That is, if it's true we're being called as a command line script;
+# if it's false, we're being sourced or otherwise included, such as for
+# library or inlinedocs.
+if( identical( environment( ), globalenv( ) ) &&
+	!length( grep( "^source\\(", sys.calls( ) ) ) ) {
+	main( pArgs ) }