Mercurial > repos > gga > apollo_fetch_jbrowse
comparison export.py @ 0:c6d7f19953a6 draft
planemo upload for repository https://github.com/galaxy-genome-annotation/galaxy-tools/tree/master/tools/apollo commit f745b23c84a615bf434d717c8c0e553a012f0268
author | gga |
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date | Mon, 11 Sep 2017 05:47:25 -0400 |
parents | |
children | 9968eec7430e |
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-1:000000000000 | 0:c6d7f19953a6 |
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1 #!/usr/bin/env python | |
2 from __future__ import print_function | |
3 | |
4 import argparse | |
5 import json | |
6 import sys | |
7 | |
8 from BCBio import GFF | |
9 | |
10 from Bio import SeqIO | |
11 | |
12 from future import standard_library | |
13 | |
14 from webapollo import CnOrGuess, GuessCn, WAAuth, WebApolloInstance | |
15 | |
16 standard_library.install_aliases() | |
17 try: | |
18 import StringIO as io | |
19 except ImportError: | |
20 import io | |
21 | |
22 | |
23 def export(org_cn, seqs): | |
24 org_data = wa.organisms.findOrganismByCn(org_cn) | |
25 | |
26 data = io.StringIO() | |
27 | |
28 kwargs = dict( | |
29 exportType='GFF3', | |
30 seqType='genomic', | |
31 exportGff3Fasta=True, | |
32 output="text", | |
33 exportFormat="text", | |
34 organism=org_cn, | |
35 ) | |
36 | |
37 if len(seqs) > 0: | |
38 data.write(wa.io.write( | |
39 exportAllSequences=False, | |
40 sequences=seqs, | |
41 **kwargs | |
42 ).encode('utf-8')) | |
43 else: | |
44 data.write(wa.io.write( | |
45 exportAllSequences=True, | |
46 sequences=[], | |
47 **kwargs | |
48 ).encode('utf-8')) | |
49 | |
50 # Seek back to start | |
51 data.seek(0) | |
52 | |
53 records = list(GFF.parse(data)) | |
54 if len(records) == 0: | |
55 print("Could not find any sequences or annotations for this organism + reference sequence") | |
56 sys.exit(2) | |
57 else: | |
58 for record in records: | |
59 record.annotations = {} | |
60 record.features = sorted(record.features, key=lambda x: x.location.start) | |
61 if args.gff: | |
62 GFF.write([record], args.gff) | |
63 record.description = "" | |
64 if args.fasta: | |
65 SeqIO.write([record], args.fasta, 'fasta') | |
66 | |
67 return org_data | |
68 | |
69 | |
70 if __name__ == '__main__': | |
71 parser = argparse.ArgumentParser(description='Sample script to add an attribute to a feature via web services') | |
72 WAAuth(parser) | |
73 CnOrGuess(parser) | |
74 parser.add_argument('--gff', type=argparse.FileType('w')) | |
75 parser.add_argument('--fasta', type=argparse.FileType('w')) | |
76 parser.add_argument('--json', type=argparse.FileType('w')) | |
77 | |
78 args = parser.parse_args() | |
79 | |
80 wa = WebApolloInstance(args.apollo, args.username, args.password) | |
81 | |
82 org_cn_list, seqs = GuessCn(args, wa) | |
83 | |
84 org_data = [] | |
85 for org_cn in org_cn_list: | |
86 indiv_org_data = export(org_cn, seqs) | |
87 org_data.append(indiv_org_data) | |
88 args.json.write(json.dumps(org_data, indent=2)) |