Mercurial > repos > gianmarco_piccinno > cs_tool_project_rm
comparison pEPSA5_annotated.gb @ 0:5397da1ef896 draft
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| author | gianmarco_piccinno |
|---|---|
| date | Tue, 21 May 2019 05:05:15 -0400 |
| parents | |
| children |
comparison
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| -1:000000000000 | 0:5397da1ef896 |
|---|---|
| 1 LOCUS Exported 6850 bp ds-DNA circular SYN 13-NOV-2018 | |
| 2 DEFINITION synthetic circular DNA | |
| 3 ACCESSION . | |
| 4 VERSION . | |
| 5 KEYWORDS . | |
| 6 SOURCE synthetic DNA construct | |
| 7 ORGANISM synthetic DNA construct | |
| 8 REFERENCE 1 (bases 1 to 6850) | |
| 9 AUTHORS . | |
| 10 TITLE Direct Submission | |
| 11 JOURNAL Exported Nov 13, 2018 from SnapGene 4.2.6 | |
| 12 http://www.snapgene.com | |
| 13 COMMENT LOCUS dna 6850 bp | |
| 14 FEATURES Location/Qualifiers | |
| 15 source 1..6850 | |
| 16 /organism="synthetic DNA construct" | |
| 17 /mol_type="other DNA" | |
| 18 misc_feature 1..819 | |
| 19 /label=p15A origin of replication region | |
| 20 /note="p15A origin of replication region" | |
| 21 misc_feature 1032..3625 | |
| 22 /label=Staphylococcus aureus plasmid pC194 | |
| 23 /note="Staphylococcus aureus plasmid pC194 region | |
| 24 (NC_002013.1)" | |
| 25 misc_feature 1126..1324 | |
| 26 /label=Repeat sequence 1 of 2 | |
| 27 /note="Repeat sequence 1 of 2" | |
| 28 CDS complement(1562..2212) | |
| 29 /label=Chloramphenicol Resistance | |
| 30 CDS complement(2346..2744) | |
| 31 /label=Potential Copy number associated protein | |
| 32 /note="Potential Copy number associated protein" | |
| 33 misc_feature 2463..2517 | |
| 34 /label=pC194 replication origin | |
| 35 /note="pC194 replication origin (detailed in PMC401278)" | |
| 36 misc_feature 2670..3605 | |
| 37 /label=Replication initiation protein Rep RC | |
| 38 misc_feature 3626..3824 | |
| 39 /label=Repeat sequence 2 of 2 | |
| 40 /note="Repeat sequence 2 of 2" | |
| 41 misc_feature 3825..5278 | |
| 42 /label=Xylose Inducible Promoter Region | |
| 43 /note="Xylose Inducible Promoter Region, Xyl promoter, Xyl | |
| 44 repressor protein, Xyl terminator sequence and pTX5 | |
| 45 promoter facing MCS: | |
| 46 http://parts.igem.org/Part:BBa_K1323014" | |
| 47 CDS 4000..5151 | |
| 48 /label=XylR repressor protein | |
| 49 misc_feature 5191..5241 | |
| 50 /label=Bacteriophage T5 PN25 promoter | |
| 51 /note="Bacteriophage T5 PN25 promoter (Deuschle et al, | |
| 52 PMC1167251)" | |
| 53 misc_feature 5206..5211 | |
| 54 /label=-30 region | |
| 55 /note="-30 region" | |
| 56 misc_feature 5229..5234 | |
| 57 /label=-10 region | |
| 58 /note="-10 region" | |
| 59 misc_feature 5241..5279 | |
| 60 /label=Xyl operator containing palindromic | |
| 61 /note="interfering with transcription initiation" | |
| 62 misc_feature 5279..5317 | |
| 63 /label=MCS | |
| 64 /note="MCS" | |
| 65 terminator 5393..5550 | |
| 66 /label=rrnB term | |
| 67 terminator 5516..5559 | |
| 68 /label=rrnB_T1 term | |
| 69 terminator 5691..5718 | |
| 70 /label=rrnB_T2 term | |
| 71 misc_feature complement(5860..6720) | |
| 72 /label=Ampicillin Resistance | |
| 73 /note="gene of the plasmid pLEX5BA" | |
| 74 promoter complement(6762..6790) | |
| 75 /label=amp prom | |
| 76 ORIGIN | |
| 77 1 ggcggccgca ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt | |
| 78 61 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc | |
| 79 121 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct | |
| 80 181 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga | |
| 81 241 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct | |
| 82 301 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc | |
| 83 361 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc | |
| 84 421 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc | |
| 85 481 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt | |
| 86 541 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact | |
| 87 601 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag | |
| 88 661 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc | |
| 89 721 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat | |
| 90 781 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatg cggccgcttc tttcctgcgt | |
| 91 841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc | |
| 92 901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc | |
| 93 961 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataggaaga tccctcgacc | |
| 94 1021 tgcaggcatg caagcttctg taggttttta ggcataaaac tatatgattt acccctaaat | |
| 95 1081 ctttaaaatg ccccttaaaa ttcaaaataa aggcatttaa aatttaaata tttcttgtga | |
| 96 1141 taaagtttgt taaaaaggag tggttttatg actgttatgt ggttatcgat tataggtatg | |
| 97 1201 tggttttgta ttggaatggc attttttgct atcaaggtta ttaaaaataa aaattagacc | |
| 98 1261 acgcatttat gccgagaaaa tttattgtgc gttgagaaga acccttaact aaacttgcag | |
| 99 1321 acgaatgtcg gcatagcgtg agctattaag ccgaccattc gacaagtttt gggattgtta | |
| 100 1381 agggttccga ggctcaacgt caataaagca attggaataa agaagcgaaa aaggagaagt | |
| 101 1441 cggttcagaa aaagaaggat atggatctgg agctgtaata taaaaacctt cttcaactaa | |
| 102 1501 cggggcaggt tagtgacatt agaaaaccga ctgtaaaaag tacagtcggc attatctcat | |
| 103 1561 attataaaag ccagtcatta ggcctatctg acaattcctg aatagagttc ataaacaatc | |
| 104 1621 ctgcatgata accatcacaa acagaatgat gtacctgtaa agatagcggt aaatatattg | |
| 105 1681 aattaccttt attaatgaat tttcctgctg taataatggg tagaaggtaa ttactattat | |
| 106 1741 tattgatatt taagttaaac ccagtaaatg aagtccatgg aataatagaa agagaaaaag | |
| 107 1801 cattttcagg tataggtgtt ttgggaaaca atttccccga accattatat ttctctacat | |
| 108 1861 cagaaaggta taaatcataa aactctttga agtcattctt tacaggagtc caaataccag | |
| 109 1921 agaatgtttt agatacacca tcaaaaattg tataaagtgg ctctaactta tcccaataac | |
| 110 1981 ctaactctcc gtcgctattg taaccagttc taaaagctgt atttgagttt atcacccttg | |
| 111 2041 tcactaagaa aataaatgca gggtaaaatt tatatccttc ttgttttatg tttcggtata | |
| 112 2101 aaacactaat atcaatttct gtggttatac taaaagtcgt ttgttggttc aaataatgat | |
| 113 2161 taaatatctc ttttctcttc caattgtcta aatcaatttt attaaagttc atttgatatg | |
| 114 2221 cctcctaaat ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag | |
| 115 2281 aatcaatcct tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc | |
| 116 2341 ccactttatc caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaaagac | |
| 117 2401 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc | |
| 118 2461 ttttctttct tatcttgata ataagggtaa ctattgccgg cgaggctagt tacccttaag | |
| 119 2521 ttattggtat gactggtttt aagcgcaaaa aaagttgctt tttcgtacct attaatgtat | |
| 120 2581 cgttttaaat gaatagtaaa aaacatacat agaaagggga aaaagcaact ttttttattg | |
| 121 2641 tcatagtttg tgaaaactaa gttgttttta tgtgttataa catggaaaag tatactgaga | |
| 122 2701 aaaaacaaag aaatcaagta tttcagaaat ttattaaacg tcatattgga gagaatcaaa | |
| 123 2761 tggatttagt tgaagattgc aatacatttc tgtcttttgt agctgataaa actttagaaa | |
| 124 2821 aacagaaatt atataaagct aattcttgta aaaatcgatt ttgtcctgtc tgtgcttgga | |
| 125 2881 gaaaagctag aaaagatgca ttgggtttat ctttgatgat gcaatatatt aagcagcaag | |
| 126 2941 agaaaaagga gtttatcttt ttaactttga ctacacctaa tgtaatgagt gatgaattag | |
| 127 3001 aaaatgaaat aaaacgttat aataattctt ttagaaaact tataaagaga aaaaaagtag | |
| 128 3061 gtagtgttat aaagggatat gttcgtaagt tagagattac atataataaa aaaagagatg | |
| 129 3121 attataatcc tcattttcat gtgttaattg cagtaaataa atcgtatttc acagataaaa | |
| 130 3181 gatattatat tagccaacaa gaatggttag atttatggcg tgatgtaacg ggcatttcag | |
| 131 3241 aaataacaca agttcaagtt caaaaaataa gacaaaataa taataaagaa ttatatgaaa | |
| 132 3301 tggctaagta ttctggtaaa gatagtgatt atttaataaa tcaaaaagtc tttgatgcat | |
| 133 3361 tttataaatc acttaaaggt aaacaggtat tagtttattc aggattattt aaagaggcta | |
| 134 3421 aaaagaaatt aaaaaatggg gatttagatt acttaaaaga aattgatcca accgaatata | |
| 135 3481 tctatcaaat tttttatatt tggaaacaaa aagagtattt agctagtgaa ctttatgact | |
| 136 3541 taacagaaca agaaaaaaga gaaattaatc acaaaatgat agacgaaatc gaggaagaac | |
| 137 3601 aataacaaaa tataagtgct aacagtcgtc tgcaagttta gttaagggtt cttctcaacg | |
| 138 3661 cacaataaat tttctcggca taaatgcgtg gtctaatttt tatttttaat aaccttgata | |
| 139 3721 gcaaaaaatg ccattccaat acaaaaccac atacctataa tcgataacca cataacagtc | |
| 140 3781 ataaaaccac tcctttttaa caaactttat cacaagaaat attttggcat tctacgacta | |
| 141 3841 taacttaaat ttatattttt tactttataa tatataattg attatagaat aatgttgctc | |
| 142 3901 atatcgtttg ccaacatcta gtactcaaat tacactatgt tacacttggt aatattaacc | |
| 143 3961 gaacttcccc tgtccaaatt agataagagg taataataaa tggaaaataa ttttatagta | |
| 144 4021 aatgaaaatg agaagcgtgt attaaaacaa attttcaata acagcaatat ttcacgaaca | |
| 145 4081 caaatatcga agaatttaga acttaataaa gctactattt ctaacattct gaacaactta | |
| 146 4141 aaacacaaga gtttagttaa tgaagtagga gaaggtaata gtactaaaag tggtggacga | |
| 147 4201 aagcctattt tactcgaaat taaccaaaaa tatggctact atatttctat ggatttaaca | |
| 148 4261 tatgattccg ttgaattaat gtacaactac tttgatgcta ctatattaaa gcaagattcc | |
| 149 4321 tacgaattaa atgataaaaa tgtaagcagt atattacaaa ttttaaaatc taatataaac | |
| 150 4381 gtctcagaaa aatatgatac gttatatggg ttacttggta tatctatatc catacacggt | |
| 151 4441 atcgttgacg atgagcaaaa cataatcaat cttccttttc ataaaaatga gaaacgcaca | |
| 152 4501 tttaccgatg aattaaagtc attcacaaat gttcctgtcg ttatagaaaa tgaagcaaat | |
| 153 4561 ttatcagcgc tatatgaaaa aagtttatat attaattcaa acataaataa tttgattact | |
| 154 4621 ttaagtattc acaagggtat aggcgctggc atcctaataa ataaaaaact ttatcgtggc | |
| 155 4681 tcaaatggag aggctggaga gataggtaag acattggttt tggaatctat aaataacaat | |
| 156 4741 gacaacaaat attataaaat cgaagatata tgctcccaag acgctttaat acagaaaata | |
| 157 4801 aataataggt tgggcgtcac attgacgttt acagaactaa tccaatatta caacgaagga | |
| 158 4861 aattcaattg ttgctcatga aattaaacaa tttattaata aaatgacagt tctgattcat | |
| 159 4921 aatttgaata cacaatttaa cccagacgct atttatatta actgtccttt aattaatgaa | |
| 160 4981 ttaccaaata ttttaaatga aattaaagag caattctcct gtttttctca aggcagtcca | |
| 161 5041 gttcaattac atttaactac taatgtaaaa caagctactt tattgggtgg cactttagca | |
| 162 5101 ataatgcaaa aaacattaaa tataaataac attcaaatga atattaaata attacagcag | |
| 163 5161 tctgagttat aaaatagata tctcggaccg tcataaaaaa tttatttgct ttcaggaaaa | |
| 164 5221 tttttctgta taatagattc aagttagttt gtttattaaa ttaaccaact aaaatgtaga | |
| 165 5281 attcgagctc ggtacccggg gatcctctag agtcgacctg cagccaagct tgggcttttc | |
| 166 5341 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc | |
| 167 5401 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc | |
| 168 5461 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa | |
| 169 5521 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc | |
| 170 5581 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg | |
| 171 5641 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat | |
| 172 5701 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt | |
| 173 5761 caaatatgta tccgctcatc cccatcctat cgatgataag ctgtcaaaca tgagaattaa | |
| 174 5821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga | |
| 175 5881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt | |
| 176 5941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg | |
| 177 6001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga | |
| 178 6061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga | |
| 179 6121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg | |
| 180 6181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc | |
| 181 6241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc | |
| 182 6301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca | |
| 183 6361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac | |
| 184 6421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg | |
| 185 6481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgctcttc | |
| 186 6541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg | |
| 187 6601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac | |
| 188 6661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat | |
| 189 6721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata | |
| 190 6781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa | |
| 191 6841 agtgccacct | |
| 192 // |