# HG changeset patch # User gkumar09 # Date 1443036093 14400 # Node ID a40a7ffdf14a7084b88841cd83d6f61e708c2eb8 # Parent 97ddd78dfa8ffa5930af5ac19505934c291d6045 Uploaded diff -r 97ddd78dfa8f -r a40a7ffdf14a align_and_estimate_abundance.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/align_and_estimate_abundance.xml Wed Sep 23 15:21:33 2015 -0400 @@ -0,0 +1,90 @@ + + + run RSEM to estimate transcript abundances + + trinity + + + + trinityToolWrapper.py util/align_and_estimate_abundance.pl --transcripts $transcripts --est_method RSEM --aln_method bowtie --trinity_mode --prep_reference + + ## Inputs. + #if str($inputs.paired_or_single) == "paired": + --left $inputs.left_input --right $inputs.right_input + #if $inputs.left_input.ext == 'fa': + --seqType fa + #else: + --seqType fq + #end if + #if str($inputs.library_type) != "None": + --SS_lib_type $inputs.library_type + #end if + + #else: + --single $inputs.input + #if str($inputs.input.ext) == 'fa': + --seqType fa + #else: + --seqType fq + #end if + #if str($inputs.library_type) != "None": + --SS_lib_type $inputs.library_type + #end if + #end if + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + .. _Trinity: http://trinityrnaseq.sourceforge.net + +