# HG changeset patch
# User goeckslab
# Date 1718045107 0
# Node ID 834ee9481948ce17388cbf63a65b334ff6431347
planemo upload for repository https://github.com/goeckslab/tools-mti/tree/main/tools/scimap commit 9fb5578191db8a559191e45156cfb95350f01aea
diff -r 000000000000 -r 834ee9481948 anndata_to_csv.py
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/anndata_to_csv.py Mon Jun 10 18:45:07 2024 +0000
@@ -0,0 +1,45 @@
+import argparse
+import json
+import warnings
+
+import scimap as sm
+from anndata import read_h5ad
+
+
+def main(inputs, outfile):
+ """
+ Parameters
+ ---------
+ inputs : str
+ File path to galaxy tool parameter.
+ anndata : str
+ File path to anndata.
+ output : str
+ File path to output.
+ """
+ warnings.simplefilter('ignore')
+
+ with open(inputs, 'r') as param_handler:
+ params = json.load(param_handler)
+
+ adata = read_h5ad(params['anndata'])
+
+ if params['layer'] == 'x':
+ params['layer'] = None
+
+ df = sm.hl.scimap_to_csv(
+ adata=adata,
+ layer=params['layer'],
+ CellID=params['cellid'],
+ )
+
+ df.to_csv(outfile, index=False)
+
+
+if __name__ == '__main__':
+ aparser = argparse.ArgumentParser()
+ aparser.add_argument("-i", "--inputs", dest="inputs", required=True)
+ aparser.add_argument("-e", "--outfile", dest="outfile", required=True)
+ args = aparser.parse_args()
+
+ main(args.inputs, args.outfile)
diff -r 000000000000 -r 834ee9481948 main_macros.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/main_macros.xml Mon Jun 10 18:45:07 2024 +0000
@@ -0,0 +1,33 @@
+
+ 2.1.0
+ 0
+ 20.01
+
+
+
+ quay.io/goeckslab/scimap:@TOOL_VERSION@
+
+
+
+
+
+
+
+
+
+
+
+ 10.1158/2159-8290.CD-21-1357
+
+
+
+
+
+
diff -r 000000000000 -r 834ee9481948 mcmicro_to_anndata.py
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/mcmicro_to_anndata.py Mon Jun 10 18:45:07 2024 +0000
@@ -0,0 +1,49 @@
+import argparse
+import json
+import warnings
+
+import scimap as sm
+
+
+def main(inputs, outfile):
+ """
+ Parameter
+ ---------
+ inputs : str
+ File path to galaxy tool parameter.
+
+ outfile : str
+ File path to estimator.
+ """
+ warnings.simplefilter('ignore')
+
+ with open(inputs, 'r') as param_handler:
+ params = json.load(param_handler)
+
+ image_path = params['image_path']
+ drop_markers = params['drop_markers']
+ if not drop_markers:
+ drop_markers = None
+ else:
+ drop_markers = [x.strip() for x in drop_markers.split(',')]
+ options = params['options']
+ for k, v in options.items():
+ if v == '':
+ options[k] = None
+
+ adata = sm.pp.mcmicro_to_scimap(
+ image_path,
+ drop_markers=drop_markers,
+ **options
+ )
+
+ adata.write(outfile)
+
+
+if __name__ == '__main__':
+ aparser = argparse.ArgumentParser()
+ aparser.add_argument("-i", "--inputs", dest="inputs", required=True)
+ aparser.add_argument("-e", "--outfile", dest="outfile", required=True)
+ args = aparser.parse_args()
+
+ main(args.inputs, args.outfile)
diff -r 000000000000 -r 834ee9481948 scimap_phenotyping.py
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/scimap_phenotyping.py Mon Jun 10 18:45:07 2024 +0000
@@ -0,0 +1,120 @@
+import argparse
+import warnings
+
+import pandas as pd
+import scimap as sm
+from anndata import read_h5ad
+
+
+def main(
+ adata,
+ output,
+ log,
+ gating_workflow,
+ gating_workflow_ext,
+ manual_gates=None,
+ manual_gates_ext=None,
+ random_state=0
+):
+ """
+ Parameter
+ ---------
+ adata : str
+ File path to the input AnnData.
+ output : str
+ File path to the output AnnData.
+ log: bool
+ Boolean whether to log the input data prior to rescaling
+ gating_workflow : str
+ File path to the gating workflow.
+ gating_workflow_ext : str
+ Datatype for gating workflow, either 'csv' or 'tabular'.
+ manual_gates : str
+ File path to the munual gating.
+ manual_gates_ext : str
+ Datatype for munual gate, either 'csv' or 'tabular'.
+ random_state: int
+ The seed used by the random number generator for GMM in sm.pp.rescale
+ """
+ warnings.simplefilter('ignore')
+
+ adata = read_h5ad(adata)
+ # Rescale data
+ if manual_gates:
+ sep = ',' if manual_gates_ext == 'csv' else '\t'
+ manual_gates = pd.read_csv(manual_gates, sep=sep)
+
+ adata = sm.pp.rescale(
+ adata,
+ gate=manual_gates,
+ log=log,
+ random_state=random_state
+ )
+
+ # Phenotype cells
+ # Load the gating workflow
+ sep = ',' if gating_workflow_ext == 'csv' else '\t'
+ phenotype = pd.read_csv(gating_workflow, sep=sep)
+ adata = sm.tl.phenotype_cells(
+ adata,
+ phenotype=phenotype,
+ label="phenotype"
+ )
+
+ # Summary of the phenotyping
+ print(adata.obs['phenotype'].value_counts())
+
+ adata.write(output)
+
+
+if __name__ == '__main__':
+ aparser = argparse.ArgumentParser()
+ aparser.add_argument("-a", "--adata", dest="adata", required=True)
+ aparser.add_argument("-o", "--output", dest="output", required=True)
+ aparser.add_argument("-l", "--log", dest="log", action="store_true")
+ aparser.add_argument(
+ "-g",
+ "--gating_workflow",
+ dest="gating_workflow",
+ required=True
+ )
+ aparser.add_argument(
+ "-s",
+ "--gating_workflow_ext",
+ dest="gating_workflow_ext",
+ required=True
+ )
+ aparser.add_argument(
+ "-m",
+ "--manual_gates",
+ dest="manual_gates",
+ required=False
+ )
+ aparser.add_argument(
+ "-S",
+ "--manual_gates_ext",
+ dest="manual_gates_ext",
+ required=False
+ )
+ aparser.add_argument(
+ "--random_state",
+ dest="random_state",
+ type=int,
+ required=False
+ )
+
+ args = aparser.parse_args()
+
+ if args.log:
+ print("\n adata.raw.X will be log1p transformed \n")
+
+ main(
+ adata=args.adata,
+ output=args.output,
+ log=args.log,
+ gating_workflow=args.gating_workflow,
+ gating_workflow_ext=args.gating_workflow_ext,
+ manual_gates=args.manual_gates,
+ manual_gates_ext=args.manual_gates_ext,
+ random_state=args.random_state
+ )
diff -r 000000000000 -r 834ee9481948 scimap_plotting.py
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/scimap_plotting.py Mon Jun 10 18:45:07 2024 +0000
@@ -0,0 +1,125 @@
+import argparse
+import json
+import os
+import warnings
+
+import matplotlib.pylab as plt
+import numpy as np
+import scimap as sm
+import seaborn as sns
+from anndata import read_h5ad
+
+sns.set(color_codes=True)
+
+
+def main(inputs, anndata, output):
+ """
+ Parameter
+ ---------
+ inputs : str
+ File path to galaxy tool parameter.
+ anndata : str
+ File path to anndata containing phenotyping info.
+ output : str
+ File path to output.
+ """
+ warnings.simplefilter('ignore')
+
+ with open(inputs, 'r') as param_handler:
+ params = json.load(param_handler)
+
+ adata = read_h5ad(anndata)
+
+ tool = params['analyses']['selected_tool']
+ options = params['analyses']['options']
+
+ if tool == 'stacked_barplot':
+
+ # parse list text arguments
+ for o in options.copy():
+ opt_list = options.pop(o)
+ if opt_list:
+ options[o] = [x.strip() for x in opt_list.split(',')]
+
+ # add base args into options dict to pass to tool
+ options['x_axis'] = params['analyses']['x_axis']
+ options['y_axis'] = params['analyses']['y_axis']
+ options['method'] = params['analyses']['method']
+
+ options['return_data'] = True
+
+ df = sm.pl.stacked_barplot(adata, **options)
+
+ # Pick cmap to use
+ num_phenotypes = len(df.columns) - 1
+ if num_phenotypes <= 9:
+ matplotlib_cmap = "Set1"
+ elif num_phenotypes > 9 and num_phenotypes <= 20:
+ matplotlib_cmap = plt.cm.tab20
+ else:
+ matplotlib_cmap = plt.cm.gist_ncar
+
+ # Plotting
+ sns.set_theme(style="white")
+ ax = df.plot.bar(stacked=True, cmap=matplotlib_cmap)
+ fig = ax.get_figure()
+ handles, labels = ax.get_legend_handles_labels()
+ ax.legend(
+ reversed(handles),
+ reversed(labels),
+ bbox_to_anchor=(1, 1),
+ loc='upper left'
+ )
+ plt.tight_layout()
+
+ # # save and close
+ fig.savefig('out.png', dpi=300)
+ plt.close(fig)
+
+ if tool == 'voronoi':
+
+ plt.style.use('fast')
+
+ tool_func = getattr(sm.pl, tool)
+
+ # x_lim/y_lim need to be parsed from comma-sep str to integer tuples
+ for lim in ['x_lim', 'y_lim']:
+ opt_list = options.pop(lim)
+ if opt_list:
+ options[lim] = [int(x.strip()) for x in opt_list.split(',')]
+
+ # parse list text arguments
+ for cat in ['overlay_points_categories', 'overlay_drop_categories']:
+ opt_list = options.pop(cat)
+ if opt_list:
+ options[cat] = [x.strip() for x in opt_list.split(',')]
+
+ # add base args into options dict to pass to tool
+ options['color_by'] = params['analyses']['color_by']
+ options['x_coordinate'] = params['analyses']['x_coordinate']
+ options['y_coordinate'] = params['analyses']['y_coordinate']
+
+ # fill any missing params with None as tool expects
+ for k, v in options.items():
+ if v == '':
+ options[k] = None
+
+ options['saveDir'] = os.getcwd()
+ options['fileName'] = 'out.png'
+
+ if options['size_max'] is None:
+ options['size_max'] = np.inf
+
+ # call the tool and unpack all options
+ tool_func(adata, **options)
+
+
+if __name__ == '__main__':
+ aparser = argparse.ArgumentParser()
+ aparser.add_argument("-i", "--inputs", dest="inputs", required=True)
+ aparser.add_argument("-a", "--anndata", dest="anndata", required=True)
+ aparser.add_argument("-e", "--output", dest="output", required=True)
+
+ args = aparser.parse_args()
+
+ main(args.inputs, args.anndata, args.output)
diff -r 000000000000 -r 834ee9481948 scimap_plotting.xml
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/scimap_plotting.xml Mon Jun 10 18:45:07 2024 +0000
@@ -0,0 +1,114 @@
+
+ from Scimap
+
+ main_macros.xml
+
+
+
+
+ echo "@VERSION@"
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diff -r 000000000000 -r 834ee9481948 scimap_spatial.py
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/scimap_spatial.py Mon Jun 10 18:45:07 2024 +0000
@@ -0,0 +1,62 @@
+import argparse
+import json
+import warnings
+
+import scimap as sm
+from anndata import read_h5ad
+
+
+def main(inputs, anndata, output):
+ """
+ Parameter
+ ---------
+ inputs : str
+ File path to galaxy tool parameter.
+ anndata : str
+ File path to anndata containing phenotyping info.
+ output : str
+ File path to output.
+ """
+ warnings.simplefilter('ignore')
+
+ with open(inputs, 'r') as param_handler:
+ params = json.load(param_handler)
+
+ adata = read_h5ad(anndata)
+
+ tool = params['analyses']['selected_tool']
+ tool_func = getattr(sm.tl, tool)
+
+ options = params['analyses']['options']
+ if tool == 'cluster':
+ options['method'] = params['analyses']['method']
+ subset_genes = options.pop('subset_genes')
+ if subset_genes:
+ options['subset_genes'] = \
+ [x.strip() for x in subset_genes.split(',')]
+ sub_cluster_group = options.pop('sub_cluster_group')
+ if sub_cluster_group:
+ options['sub_cluster_group'] = \
+ [x.strip() for x in sub_cluster_group.split(',')]
+
+ for k, v in options.items():
+ if v == '':
+ options[k] = None
+
+ tool_func(adata, **options)
+
+ if tool == 'spatial_lda':
+ adata.uns.pop('spatial_lda_model')
+
+ adata.write(output)
+
+
+if __name__ == '__main__':
+ aparser = argparse.ArgumentParser()
+ aparser.add_argument("-i", "--inputs", dest="inputs", required=True)
+ aparser.add_argument("-e", "--output", dest="output", required=True)
+ aparser.add_argument("-a", "--anndata", dest="anndata", required=True)
+
+ args = aparser.parse_args()
+
+ main(args.inputs, args.anndata, args.output)
diff -r 000000000000 -r 834ee9481948 test-data/manual_gates.csv
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/manual_gates.csv Mon Jun 10 18:45:07 2024 +0000
@@ -0,0 +1,19 @@
+markers,gate
+ASMA,6.6
+CD163,6.6
+CD206,7.5
+CD68,6.2
+CD20,7.8
+CD21,8
+CD3D,6.5
+CD45,7.3
+CD56,8.7
+CD8A,6.5
+FOXP3,5.2
+CD11B,6.7
+CD11C,6.3
+CD15,7.9
+CD4,7.4
+PD1,7.9
+HLADR,6.7
+CD25,7
\ No newline at end of file
diff -r 000000000000 -r 834ee9481948 test-data/mcmicro_output.csv
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/mcmicro_output.csv Mon Jun 10 18:45:07 2024 +0000
@@ -0,0 +1,500 @@
+CellID,DNA1,BG1,BG2,BG3,DNA2,CD25,CD2,CD10,DNA3,CD163,CD3D,CD5,DNA4,CD30,ACTIN,CD45,DNA5,CD206,CD68,PD1,DNA6,KI67,CD11C,CD7,DNA7,CD8A,FOXP3,CD20,DNA8,CD4,HLADR,PSTAT3,DNA9,PS6,PERK,CD21,DNA10,CD43,NOS2,PDL1,DNA11,CD11B,CD57,MHCI,DNA12,CD15,ASMA,CD56,X_centroid,Y_centroid,Area,MajorAxisLength,MinorAxisLength,Eccentricity,Solidity,Extent,Orientation
+1,16640.5641,719.6324786,527.7094017,1100.273504,14726.17094,581.5811966,618.3846154,1606.777778,7182.247863,509.3247863,477.5897436,1795.410256,10677.03419,1084.051282,5382.076923,2983.239316,3771.91453,1103.111111,399.3418803,2705.57265,7494.65812,1599.940171,757.7692308,451.6324786,8504.008547,389.8547009,109.042735,7809.042735,7239.435897,1408.564103,807.2393162,2814.196581,5718.74359,617.4273504,260.0598291,20362.76923,3721.222222,756.3589744,370.4615385,1334.666667,3247.74359,969.8034188,148.5726496,4133.598291,2508.555556,1085.735043,218.5470085,3170.470085,511.5555556,9.846153846,117,14.53227038,10.27362788,0.707260822,0.959016393,0.75,-0.695369483
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+5,17922.32934,685.9580838,521.6646707,1075.023952,15913.23952,562,524.4550898,1596.982036,8090.39521,482.0658683,372.8982036,1808.51497,11358.23952,1041.275449,3203.676647,2656.94012,4390.658683,1079.97006,315.6467066,2396.39521,7671.389222,2875.526946,122.8742515,427.508982,9384.772455,372.9041916,101.2754491,4407.814371,7981.209581,1239.616766,825.754491,2779.275449,6312.275449,514.5928144,251.3353293,18157.99401,4689.820359,725.508982,350.5628743,1326.952096,3575.724551,838.2155689,144.2275449,2677.179641,2866.928144,1107.874251,157.6047904,3074.137725,657.1736527,18.03592814,167,17.67583101,12.11010632,0.728428039,0.943502825,0.695833333,-0.810889758
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diff -r 000000000000 -r 834ee9481948 test-data/phenotype_workflow.csv
--- /dev/null Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/phenotype_workflow.csv Mon Jun 10 18:45:07 2024 +0000
@@ -0,0 +1,23 @@
+,,ASMA,CD163,CD206,CD68,CD20,CD21,CD3D,CD45,CD56,CD8A,FOXP3,CD11B,CD11C,CD15,CD4,PD1,HLADR,CD25
+all,Other Immune cells,,,,,,,,pos,,,,,,,,,,
+all,ASMA+ cells,pos,,,,,,,,,,,,,,,,,
+Other Immune cells,T cells,,,,,,,pos,,,,,,,,,,,
+Other Immune cells,B cells,,,,,pos,,,,,,,,,,,,,
+Other Immune cells,Myeloid Lineage,,anypos,anypos,anypos,,,,,,,,anypos,anypos,,,,,
+Other Immune cells,NK cells,,,,,,,,,pos,,,,,,,,,
+Other Immune cells,Granulocytes,,,,,,,,,,,,,,pos,,,,
+Myeloid Lineage,T cells,,,,,,,pos,,,,,,,,,,,
+Myeloid Lineage,B cells,,,,,pos,,,,,,,,,,,,,
+Myeloid Lineage,NK cells,,,,,,,,,pos,,,,,,,,,
+Myeloid Lineage,Granulocytes,,,,,,,,,,,,,,pos,,,,
+T cells,CD4 T cells,,,,,,,,,,,,,,,pos,,,
+T cells,CD8 T cells,,,,,,,,,,pos,,,,,,,,
+CD4 T cells,Regulatory T cells,,,,,,,,,,,pos,,,,,,,
+CD4 T cells,Follicular Helper T cells,,,,,,,,,,,,,,,,pos,,
+CD8 T cells,PD1+ T cells,,,,,,,,,,,,,,,,pos,,
+Myeloid Lineage,CD68+ Macrophages,,,,pos,,,,,,,,,,,,,,
+Myeloid Lineage,M2 Macrophages,,anypos,anypos,,,,,,,,,,,,,,,
+Myeloid Lineage,Myeloid Dendritic cells,,,,,,,,,,,,,,,,,pos,
+Myeloid Lineage,Follicular Dendritic cells,,,,,,pos,,,,,,,,,,,,
+CD68+ Macrophages,M1 Macrophages,,,,,,,,,,,,,,,,,pos,
+Myeloid Dendritic cells,CD25+ Dendritic cells,,,,,,,,,,,,,,,,,,pos
\ No newline at end of file
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