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author | gpovysil |
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date | Wed, 23 May 2018 08:07:28 -0400 |
parents | 95443b3fb0e4 |
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<?xml version="1.0" encoding="UTF-8"?> <tool id="range2tag" name="Duplex Sequencing Analysis: range2tag" version="0.0.1"> <requirements> <requirement type="package" version="2.7">python</requirement> <requirement type="package" version="1.13.3">numpy</requirement> </requirements> <description>Tool that extracts tags of reads that are within user-specified regions</description> <command> python2 $__tool_directory__/range2tag.py "$file1" "$file2" "$output" </command> <inputs> <param name="file1" type="data" format="sam" label="Dataset 1: SAM file with aligned reads." optional="false" help="SAM File with reads aligned to reference."/> <param name="file2" type="data" format="txt" label="Dataset 2: TXT file with start and stop positions of regions." optional="false" help="Tab delimited TXT file with start and stop positions of regions."/> </inputs> <outputs> <data name="output" format="txt" /> </outputs> <help> <![CDATA[ **What it does** This tool takes a SAM file and a TXT file with start and stop positions as input and prints all tags of reads that overlap with regions to user specified output file. **Input** **Dataset 1:** SAM file of aligned reads. **Dataset 2:** Tab delimited TXT file with start and stop positions. +-------------+--------+ | 90 | 633 | +-------------+--------+ | 659 | 1140 | +-------------+--------+ | 1144 | 1561 | +-------------+--------+ **Output** The output is a blank delimited TXT file of tags of all reads that overlap the user-specified regions with start_stop in the first column and the tag in the second column. +-----------+------------------------------+ | 87_636 | AAATCAAAGTATGAATGAAGTTGCCT | +-----------+------------------------------+ | 87_636 | AAATTCATAGCATTAATTTCAACGGG | +-----------+------------------------------+ | 656_1143 | GGGGCAGCCATATTGGCAATTATCAT | +-----------+------------------------------+ **About Author** Author: Gundula Povysil, MD, PhD Department: Institute of Bioinformatics, Johannes Kepler University Linz, Austria Contact: gpovysil@gmail.com ]]> </help> <citations> <citation type="bibtex"> @misc{duplex, author = {Heinzl, Monika}, year = {2018}, title = {Development of algorithms for the analysis of duplex sequencing data} } </citation> </citations> </tool>