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author | hepcat72 |
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date | Fri, 10 Aug 2018 13:15:57 -0400 |
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<tool id="extractSplitReads_BwaMem" name="Extract Split Reads" version="0.1.0"> <description>from bwaMEM (part of lumpy-sv)</description> <requirements> <requirement type="package" version="0.2.14a">lumpy-sv</requirement> </requirements> <version_command>extractSplitReads_BwaMem | grep ^extractSplitReads_BwaMem | cut -d v -f 2</version_command> <command detect_errors="aggressive"> <![CDATA[ extractSplitReads_BwaMem -i '$input' -n $numSplits $includeDups -m $minNonOverlap > '$output' ]]> </command> <inputs> <param format="sam" name="input" type="data" label="Sam alignment file" argument="--infile"/> <param name="numSplits" label="numSplits" argument="--numSplits" type="integer" value="2" help="The maximum number of split-read mappings to allow per read. Reads with more are excluded." /> <param name="includeDups" label="includeDups" argument="--includeDups" type="boolean" truevalue="--includeDups" falsevalue="" checked="no" value="false" help="Include alignments marked as duplicates." /> <param name="minNonOverlap" label="minNonOverlap" argument="--minNonOverlap" type="integer" value="20" help="Minimum non-overlap between split alignments on the query." /> </inputs> <outputs> <data format="sam" name="output" /> </outputs> <tests> <test> <param name="input" value="JS01-BWA-MEM.splitters.sam"/> <output name="output" file="JS01-BWA-MEM.splitters.out.sam"/> </test> <test> <param name="input" value="pe.pos_sorted.sam"/> <output name="output" file="pe.pos_sorted.out.sam"/> </test> </tests> <help> <![CDATA[ Usage: `extractSplitReads_BwaMem -i <file>` extractSplitReads_BwaMem v0.1.0 Author: Ira Hall Description: Get split-read alignments from bwa-mem in lumpy compatible format. Ignores reads marked as duplicates. Works on read or position sorted SAM input. Tested on bwa mem v0.7.5a-r405. Options: -h, --help show this help message and exit -i FILE, --inFile=FILE A SAM file or standard input (-i stdin). -n INT, --numSplits=INT The maximum number of split-read mappings to allow per read. Reads with more are excluded. Default=2 -d, --includeDups Include alignments marked as duplicates. Default=False -m INT, --minNonOverlap=INT minimum non-overlap between split alignments on the query (default=20) ]]> </help> <citations> <citation type="doi">doi:10.1186/gb-2014-15-6-r84</citation> </citations> </tool>