diff mutspecNmf_wrapper.sh @ 0:8c682b3a7c5b draft

Uploaded
author iarc
date Tue, 19 Apr 2016 03:07:11 -0400
parents
children 916846f73e25
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/mutspecNmf_wrapper.sh	Tue Apr 19 03:07:11 2016 -0400
@@ -0,0 +1,94 @@
+#!/bin/bash
+
+#########################################
+###     SPECIFY THE NUMBER OF CPU     ###
+#########################################
+cpu=1
+
+
+
+
+html=$1;shift
+parameters=$1;shift
+source=$1;shift
+input=$1
+
+if [[ $source == "html" ]]
+then input=${input%%.*}_files/Mutational_Analysis/Figures/Input_NMF/Input_NMF_Count.txt
+fi
+
+output_dir=${html%%.*}_files
+mkdir $output_dir
+
+Rscript $SCRIPT_PATH/R/somaticSignature_Galaxy.r $parameters --cpu $cpu --input $input --output $output_dir 2>&1
+
+
+## Test the existence of the files and graphs produced by NMF
+if [[ ! -e "$output_dir/NMF/Files/MatrixW-Normto100.txt" ]]; then
+	>&2 echo "error"
+	exit
+fi
+
+
+echo "<html><body>" >> $html
+echo "<center> <h2> NMF Mutational signatures analysis </h2> </center>" >> $html
+
+
+echo "<table>" >> $html
+echo "<tr> <br/> <th><h3>Heatmap of the mixture coefficient matrix</h3></th> </tr>" >> $html
+echo "<tr> <td> <center> <br/> <a href="NMF/Files/Cluster_MixtureCoeff.txt">Cluster_MixtureCoeff.txt</a> </center> </td> </tr>" >> $html
+echo "<tr>" >> $html
+
+if [[ ! -e "$output_dir/NMF/Figures/Heatmap_MixtureCoeff.png" ]]; then
+	echo "WARNING: NMF package can't plot the heatmap when the samples size is above 300. <br/>" >> $html
+else
+	echo "<td> <center> <a href="NMF/Figures/Heatmap_MixtureCoeff.png">" >> $html
+	echo "<img src="NMF/Figures/Heatmap_MixtureCoeff.png" /></a> <center> </td>" >> $html
+fi
+echo "</tr>" >> $html
+echo "</table>" >> $html
+
+echo "<br/><br/>" >> $html
+
+echo "<table>" >> $html
+echo "<tr>" >> $html
+echo "<th><h3>Signature composition</h3></th>" >> $html
+echo "</tr>" >> $html
+echo "<tr><td> <center> <a href="NMF/Files/MatrixW-Normto100.txt">Composition somatic mutation (input matrix for the tool MutSpec-Compare)</a><center></td></tr>" >> $html
+echo "<tr>" >> $html
+echo "<td><a href="NMF/Figures/CompositionSomaticMutation.png">" >> $html
+echo "<img width="1000" src="NMF/Figures/CompositionSomaticMutation.png" /></a></td>" >> $html
+echo "</tr>	" >> $html
+echo "</table>" >> $html
+echo "<br/><br/>" >> $html
+
+echo "<table>" >> $html
+echo "<tr>" >> $html
+echo "<th><h3>Sample contribution to signatures</h3></th>" >> $html
+echo "</tr>" >> $html
+echo "<tr><td> <center> <a href="NMF/Files/MatrixH-Inputggplot2.txt">Contribution mutation signature matrix</a></center></td></tr>" >> $html
+echo "<tr>" >> $html
+echo "<td><a href="NMF/Figures/ContributionMutationSignature.png">" >> $html
+echo "<img width="700" src="NMF/Figures/ContributionMutationSignature.png" /></a></td>" >> $html
+echo "</tr>	" >> $html
+echo "</table>" >> $html
+echo "<br/><br/>" >> $html
+
+
+echo "<table>" >> $html
+echo "<tr>" >> $html
+echo "<th><h3>Average contributions of each signatures in each cluster</h3></th>" >> $html
+echo "</tr>" >> $html
+echo "<tr><td> <center> <a href="NMF/Files/Average_ContriByCluster.txt">Average contributions</a></center></td></tr>" >> $html
+echo "<tr>" >> $html
+echo "<td><a href="NMF/Figures/Average_ContriByCluster.png">" >> $html
+echo "<img width="700" src="NMF/Figures/Average_ContriByCluster.png" /></a></td>" >> $html
+echo "</tr>	" >> $html
+echo "</table>" >> $html
+echo "<br/><br/>" >> $html
+
+echo "<br/><br/><br/><br/>" >> $html
+
+
+
+exit 0