Mercurial > repos > imgteam > 2d_auto_threshold
comparison auto_threshold.py @ 7:e5c8e7e72373 draft
planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_auto_threshold/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
author | imgteam |
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date | Thu, 04 Apr 2024 15:23:18 +0000 |
parents | 8bccb36e055a |
children | 699a5e9146b3 |
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6:8bccb36e055a | 7:e5c8e7e72373 |
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25 'loc_median': lambda img_raw, bz, **kwargs: skimage.filters.threshold_local(img_raw, bz, method='median'), | 25 'loc_median': lambda img_raw, bz, **kwargs: skimage.filters.threshold_local(img_raw, bz, method='median'), |
26 'loc_mean': lambda img_raw, bz, **kwargs: skimage.filters.threshold_local(img_raw, bz, method='mean') | 26 'loc_mean': lambda img_raw, bz, **kwargs: skimage.filters.threshold_local(img_raw, bz, method='mean') |
27 } | 27 } |
28 | 28 |
29 | 29 |
30 def do_thresholding(in_fn, out_fn, th_method, block_size=5, threshold=0, invert_output=False): | 30 def do_thresholding(in_fn, out_fn, th_method, block_size, offset, threshold, invert_output=False): |
31 img = skimage.io.imread(in_fn) | 31 img = skimage.io.imread(in_fn) |
32 th = th_methods[th_method](img_raw=img, bz=block_size, thres=threshold) | 32 img = np.squeeze(img) |
33 assert img.ndim == 2 | |
34 | |
35 th = offset + th_methods[th_method](img_raw=img, bz=block_size, thres=threshold) | |
33 res = img > th | 36 res = img > th |
34 if invert_output: | 37 if invert_output: |
35 res = np.logical_not(res) | 38 res = np.logical_not(res) |
39 | |
36 tifffile.imwrite(out_fn, skimage.util.img_as_ubyte(res)) | 40 tifffile.imwrite(out_fn, skimage.util.img_as_ubyte(res)) |
37 | 41 |
38 | 42 |
39 if __name__ == "__main__": | 43 if __name__ == "__main__": |
40 parser = argparse.ArgumentParser(description='Automatic Image Thresholding') | 44 parser = argparse.ArgumentParser(description='Automatic image thresholding') |
41 parser.add_argument('im_in', help='Path to the input image') | 45 parser.add_argument('im_in', help='Path to the input image') |
42 parser.add_argument('im_out', help='Path to the output image (TIFF)') | 46 parser.add_argument('im_out', help='Path to the output image (uint8)') |
43 parser.add_argument('th_method', choices=th_methods.keys(), help='Thresholding method') | 47 parser.add_argument('th_method', choices=th_methods.keys(), help='Thresholding method') |
44 parser.add_argument('block_size', type=int, default=5, help='Odd size of pixel neighborhood for calculating the threshold') | 48 parser.add_argument('block_size', type=int, default=5, help='Odd size of pixel neighborhood for calculating the threshold') |
45 parser.add_argument('threshold', type=float, default=0, help='Manual thresholding value') | 49 parser.add_argument('offset', type=float, default=0, help='Offset of automatically determined threshold value') |
50 parser.add_argument('threshold', type=float, default=0, help='Manual threshold value') | |
46 parser.add_argument('--invert_output', default=False, action='store_true', help='Values below/above the threshold are labeled with 0/255 by default, and with 255/0 if this argument is used') | 51 parser.add_argument('--invert_output', default=False, action='store_true', help='Values below/above the threshold are labeled with 0/255 by default, and with 255/0 if this argument is used') |
47 args = parser.parse_args() | 52 args = parser.parse_args() |
48 | 53 |
49 do_thresholding(args.im_in, args.im_out, args.th_method, args.block_size, args.threshold, args.invert_output) | 54 do_thresholding(args.im_in, args.im_out, args.th_method, args.block_size, args.offset, args.threshold, args.invert_output) |