view auto_threshold.py @ 8:699a5e9146b3 draft default tip

planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_auto_threshold/ commit c86a1b93cb7732f7331a981d13465653cc1a2790

"""
Copyright 2017-2024 Biomedical Computer Vision Group, Heidelberg University.

Distributed under the MIT license.
See file LICENSE for detail or copy at https://opensource.org/licenses/MIT
"""

import argparse

import giatools.io
import numpy as np
import skimage.filters
import skimage.util
import tifffile


th_methods = {
    'manual': lambda thres, **kwargs: thres,

    'otsu': lambda img_raw, **kwargs: skimage.filters.threshold_otsu(img_raw),
    'li': lambda img_raw, **kwargs: skimage.filters.threshold_li(img_raw),
    'yen': lambda img_raw, **kwargs: skimage.filters.threshold_yen(img_raw),
    'isodata': lambda img_raw, **kwargs: skimage.filters.threshold_isodata(img_raw),

    'loc_gaussian': lambda img_raw, bz, **kwargs: skimage.filters.threshold_local(img_raw, bz, method='gaussian'),
    'loc_median': lambda img_raw, bz, **kwargs: skimage.filters.threshold_local(img_raw, bz, method='median'),
    'loc_mean': lambda img_raw, bz, **kwargs: skimage.filters.threshold_local(img_raw, bz, method='mean')
}


def do_thresholding(in_fn, out_fn, th_method, block_size, offset, threshold, invert_output=False):
    img = giatools.io.imread(in_fn)
    img = np.squeeze(img)
    assert img.ndim == 2

    th = offset + th_methods[th_method](img_raw=img, bz=block_size, thres=threshold)
    res = img > th
    if invert_output:
        res = np.logical_not(res)

    tifffile.imwrite(out_fn, skimage.util.img_as_ubyte(res))


if __name__ == "__main__":
    parser = argparse.ArgumentParser(description='Automatic image thresholding')
    parser.add_argument('im_in', help='Path to the input image')
    parser.add_argument('im_out', help='Path to the output image (uint8)')
    parser.add_argument('th_method', choices=th_methods.keys(), help='Thresholding method')
    parser.add_argument('block_size', type=int, default=5, help='Odd size of pixel neighborhood for calculating the threshold')
    parser.add_argument('offset', type=float, default=0, help='Offset of automatically determined threshold value')
    parser.add_argument('threshold', type=float, default=0, help='Manual threshold value')
    parser.add_argument('--invert_output', default=False, action='store_true', help='Values below/above the threshold are labeled with 0/255 by default, and with 255/0 if this argument is used')
    args = parser.parse_args()

    do_thresholding(args.im_in, args.im_out, args.th_method, args.block_size, args.offset, args.threshold, args.invert_output)