Mercurial > repos > imgteam > imagej2_find_maxima
comparison imagej2_adjust_threshold_binary_jython_script.py @ 3:4409f9eb9433 draft default tip
planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 8f49f3c66b5a1de99ec15e65c2519a56792f1d56
author | imgteam |
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date | Wed, 25 Sep 2024 15:58:30 +0000 |
parents | 6a07f71806bb |
children |
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2:6a07f71806bb | 3:4409f9eb9433 |
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1 import sys | 1 import sys |
2 | 2 |
3 from ij import IJ | 3 from ij import IJ, Prefs |
4 | 4 |
5 # Fiji Jython interpreter implements Python 2.5 which does not | 5 # Fiji Jython interpreter implements Python 2.5 which does not |
6 # provide support for argparse. | 6 # provide support for argparse. |
7 error_log = sys.argv[-10] | 7 input_file = sys.argv[-8] |
8 input_file = sys.argv[-9] | 8 threshold_min = float(sys.argv[-7]) |
9 threshold_min = float(sys.argv[-8]) | 9 threshold_max = float(sys.argv[-6]) |
10 threshold_max = float(sys.argv[-7]) | 10 method = sys.argv[-5] |
11 method = sys.argv[-6] | 11 display = sys.argv[-4] |
12 display = sys.argv[-5] | 12 black_background = sys.argv[-3] == "yes" |
13 black_background = sys.argv[-4] == "yes" | |
14 # TODO: this is not being used. | |
15 stack_histogram = sys.argv[-3] == "yes" | |
16 output_filename = sys.argv[-2] | 13 output_filename = sys.argv[-2] |
17 output_datatype = sys.argv[-1] | 14 output_datatype = sys.argv[-1] |
18 | 15 |
19 # Open the input image file. | 16 # Open the input image file. |
20 input_image_plus = IJ.openImage(input_file) | 17 input_image_plus = IJ.openImage(input_file) |
21 | 18 |
22 # Create a copy of the image. | 19 # Create a copy of the image. |
23 input_image_plus_copy = input_image_plus.duplicate() | 20 input_image_plus_copy = input_image_plus.duplicate() |
24 image_processor_copy = input_image_plus_copy.getProcessor() | |
25 | 21 |
26 # Convert image to binary if necessary. | 22 bit_depth = input_image_plus_copy.getBitDepth() |
27 if not image_processor_copy.isBinary(): | 23 |
28 # Convert the image to binary grayscale. | |
29 IJ.run( | |
30 input_image_plus_copy, | |
31 "Make Binary", | |
32 "iterations=1 count=1 edm=Overwrite do=Nothing", | |
33 ) | |
34 # Set the options. | |
35 if black_background: | 24 if black_background: |
36 method_str = "%s dark" % method | 25 Prefs.blackBackground = True |
37 else: | 26 else: |
38 method_str = method | 27 Prefs.blackBackground = False |
39 IJ.setAutoThreshold(input_image_plus_copy, method_str) | 28 |
29 if method != "Manual": | |
30 # Set the options. | |
31 if black_background: | |
32 method_str = "%s dark" % method | |
33 suffix = "black" | |
34 else: | |
35 method_str = method | |
36 suffix = "" | |
37 threshold_min = 1 | |
38 # Set threshold_max based on image bit-depth | |
39 # For 8-bit images, use 255; for 16-bit images, use 65535 | |
40 if bit_depth == 8: | |
41 threshold_max = 255 # Default for 8-bit images | |
42 elif bit_depth == 16: | |
43 threshold_max = 65535 # Default for 16-bit images | |
44 else: | |
45 threshold_max = float('inf') # General fallback if bit depth is unknown | |
46 | |
47 IJ.setAutoThreshold(input_image_plus_copy, method_str) | |
48 IJ.run(input_image_plus_copy, "Convert to Mask", "calculate %s" % suffix) | |
40 if display == "red": | 49 if display == "red": |
41 display_mode = "Red" | 50 display_mode = "Red" |
42 elif display == "bw": | 51 elif display == "bw": |
43 display_mode = "Black & White" | 52 display_mode = "Black & White" |
44 elif display == "over_under": | 53 elif display == "over_under": |
45 display_mode = "Over/Under" | 54 display_mode = "Over/Under" |
46 IJ.setThreshold(input_image_plus_copy, threshold_min, threshold_max, display_mode) | 55 IJ.setThreshold(input_image_plus_copy, threshold_min, threshold_max, display_mode) |
47 # Run the command. | |
48 IJ.run(input_image_plus_copy, "threshold", "") | |
49 # Save the ImagePlus object as a new image. | 56 # Save the ImagePlus object as a new image. |
50 IJ.saveAs(input_image_plus_copy, output_datatype, output_filename) | 57 IJ.saveAs(input_image_plus_copy, output_datatype, output_filename) |