# HG changeset patch # User imgteam # Date 1712244398 0 # Node ID 1faa7e3c94ff26ac084f1ab32ffe6110fc45481d # Parent f312d414f234a167c5d5a86ca24c492817d6f724 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/slice_image/ commit c045f067a57e8308308cf6329060c7ccd3fc372f diff -r f312d414f234 -r 1faa7e3c94ff creators.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/creators.xml Thu Apr 04 15:26:38 2024 +0000 @@ -0,0 +1,23 @@ + + + + + + + + + + + + + + + + + + + + + + + diff -r f312d414f234 -r 1faa7e3c94ff slice_image.py --- a/slice_image.py Mon Nov 13 22:12:22 2023 +0000 +++ b/slice_image.py Thu Apr 04 15:26:38 2024 +0000 @@ -9,12 +9,9 @@ import skimage.util -def slice_image(input_file, out_folder, label=None, label_out_folder=None, window_size=64, - stride=1, bg_thresh=1, limit_slices=False, n_thresh=5000, seed=None): - # TODO NOT Implemented:process labels - # --> label and label_out_folder useless so far +def slice_image(input_file, out_folder, window_size=64, stride=1, bg_thresh=1, limit_slices=False, n_thresh=5000, seed=None): - # primarily for testing purposes: + # Primarily for testing purposes if seed is not None: random.seed(seed) @@ -40,7 +37,7 @@ sum_image = skimage.util.img_as_uint(sum_image) g = skimage.feature.greycomatrix(sum_image, [1, 2], [0, np.pi / 2], nnormed=True, symmetric=True) hom = np.var(skimage.feature.greycoprops(g, prop='homogeneity')) - if hom > bg_thresh: # 0.0005 + if hom > bg_thresh: continue if limit_slices: @@ -54,19 +51,22 @@ if __name__ == "__main__": parser = argparse.ArgumentParser() - parser.add_argument('input_file', type=argparse.FileType('r'), help='input file') - parser.add_argument('out_folder', help='out folder') - parser.add_argument('--label', dest='label_file', default=None, help='auxiliary label file to split in the same way') - parser.add_argument('--label_out_folder', dest='label_out_folder', default=None, help='label out folder') - parser.add_argument('--stride', dest='stride', type=int, default=1, help='applied stride') - parser.add_argument('--window_size', dest='window_size', type=int, default=64, help='size of resulting patches') - parser.add_argument('--bg_thresh', dest='bg_thresh', type=float, default=0, help='skip patches without information using a treshold') - parser.add_argument('--limit_slices', dest='limit_slices', type=bool, default=False, help='limit amount of slices') - parser.add_argument('--n_thresh', dest='n_thresh', type=int, default=5000, help='amount of slices') - parser.add_argument('--seed', dest='seed', type=int, default=None, help='seed for random choice of limited slices') + parser.add_argument('input_file', type=argparse.FileType('r'), help='Input file') + parser.add_argument('out_folder', help='Output directory') + parser.add_argument('--stride', dest='stride', type=int, default=1, help='Applied stride') + parser.add_argument('--window_size', dest='window_size', type=int, default=64, help='Size of resulting patches') + parser.add_argument('--bg_thresh', dest='bg_thresh', type=float, default=0, help='Skip background patches without information using a treshold') + parser.add_argument('--n_thresh', dest='n_thresh', type=int, default=5000, help='Maximum number of slices to retain') + parser.add_argument('--seed', dest='seed', type=int, default=None, help='Seed for random choice of slices') args = parser.parse_args() - slice_image(args.input_file.name, args.out_folder, - label=args.label_file, label_out_folder=args.label_out_folder, - stride=args.stride, window_size=args.window_size, bg_thresh=args.bg_thresh, - limit_slices=args.limit_slices, n_thresh=args.n_thresh, seed=args.seed) + slice_image( + args.input_file.name, + args.out_folder, + stride=args.stride, + window_size=args.window_size, + bg_thresh=args.bg_thresh, + limit_slices=args.n_thresh > 0, + n_thresh=args.n_thresh, + seed=args.seed, + ) diff -r f312d414f234 -r 1faa7e3c94ff slice_image.xml --- a/slice_image.xml Mon Nov 13 22:12:22 2023 +0000 +++ b/slice_image.xml Thu Apr 04 15:26:38 2024 +0000 @@ -1,5 +1,12 @@ - + + + creators.xml + tests.xml + + + + operation_3443 @@ -12,34 +19,35 @@ scipy tifffile - - 0: + --n_thresh '$n_thresh' #end if - #if $control_rng: - --seed $seed + + #if len('$seed') > 0: + --seed '$seed' #end if + && ls -l ./out - ]]> - + + ]]> - - - - - - - - - + + + + + + @@ -47,25 +55,38 @@ + - - - - - - - + + + + + + - **What it does** + + **Slices an image into multiple smaller, square-shaped patches.** + + For overlapping patches, set the stride to a value smaller than the patch size. + For non-overlapping patches, set the stride to a value identical to the patch size (or larger). + If the stride is set to a value larger than the patch size, parts of the original image will be skipped. - Slices image into multiple smaller patches. + Optionally, patches entirely corresponding to image background are discarded. + To decide whether a patch corresponds to image background, the `homogeneity`_ of its `gray-level co-occurrence matrix`_ is considered. + + .. _homogeneity: https://scikit-image.org/docs/stable/api/skimage.feature.html#skimage.feature.graycoprops + .. _gray-level co-occurrence matrix: https://scikit-image.org/docs/stable/api/skimage.feature.html#skimage.feature.graycomatrix + + In addition, the number of the remaining patches can be reduced by specifying a maximum number of patches to retain. + Those will be selected randomly. + 10.1016/j.jbiotec.2017.07.019 diff -r f312d414f234 -r 1faa7e3c94ff tests.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tests.xml Thu Apr 04 15:26:38 2024 +0000 @@ -0,0 +1,95 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +