diff protein_prophet.xml @ 0:a929e27eb203 draft

Uploaded

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/protein_prophet.xml	Thu Jun 21 22:30:48 2012 -0400
@@ -0,0 +1,54 @@
+<tool id="proteomics_search_protein_prophet_1" name="Protein Prophet" version="1.0.0">
+	<requirements><requirement type="package">protk</requirement></requirements>
+  <description>Calculate Protein Prophet statistics on search results</description>
+
+
+<!-- Note .. the input file is assumed to be the first argument -->
+  <command interpreter="ruby">protein_prophet_wrapper.rb $output $input_file -r $iproph $nooccam $groupwts $normprotlen $logprobs $confem $allpeps $unmapped $instances $delude --minprob=$minprob --minindep=$minindep </command>
+  <inputs>
+	
+    <param name="input_file" type="data" format="pepxml" multiple="false" label="Peptide Prophet Results" help="These files will typically be outputs from peptide prophet or interprophet"/>
+
+
+	<param name="iproph" selected="true" type="boolean" label="Inputs are from iProphet" truevalue="--iprophet-input" falsevalue=""/>
+	<param name="nooccam" type="boolean" label="Don't apply Occam's razor" help="When selected no attempt will be made to derive the simplest protein list explaining observed peptides" truevalue="--no-occam" falsevalue=""/>
+	<param name="groupwts" type="boolean" label="Use group weights" help="Check peptide's total weight (rather than actual weight) in the Protein Group against the threshold" truevalue="--group-wts" falsevalue=""/>
+	<param name="normprotlen" type="boolean" label="Normalize NSP using Protein Length" truevalue="--norm-protlen" falsevalue=""/>
+	<param name="logprobs" type="boolean" label="Use the log of probability in the confidence calculations" truevalue="--log-prob" falsevalue=""/>
+	<param name="confem" type="boolean" label="Use the EM to compute probability given the confidenct" truevalue="--confem" falsevalue=""/>
+	<param name="allpeps" type="boolean" label="Consider all possible peptides in the database in the confidence model" truevalue="--allpeps" falsevalue=""/>
+	<param name="unmapped" type="boolean" label="Report results for unmapped proteins" truevalue="--unmapped" falsevalue=""/>
+	<param name="instances" type="boolean" label="Use Expected Number of Ion Instances to adjust the peptide probabilities prior to NSP adjustment" truevalue="--instances" falsevalue=""/>
+	<param name="delude" type="boolean" label="Do NOT use peptide degeneracy information when assessing proteins" truevalue="--delude" falsevalue=""/>
+
+	<param name="minprob" type="text" label="Minimum peptide prophet probability for peptides to be considered" value="0.05"/>
+	<param name="minindep" type="text" label="Minimum percentage of independent peptides required for a protein" value="0"/>
+	
+  </inputs>
+  <outputs>
+    <data format="protxml" name="output" metadata_source="input_file" label="protein_prophet.${input_file.display_name}.protXML" from_work_dir="protein_prophet_results.prot.xml"/>
+  </outputs>
+
+
+<!--NOPLOT: do not generate plot png file
+		NOOCCAM: non-conservative maximum protein list
+		GROUPWTS: check peptide's total weight in the Protein Group against the threshold (default: check peptide's actual weight against threshold)   
+		NORMPROTLEN: Normalize NSP using Protein Length
+		LOGPROBS: Use the log of the probabilities in the Confidence calculations
+		CONFEM: Use the EM to compute probability given the confidence 
+		ALLPEPS: Consider all possible peptides in the database in the confidence model
+		UNMAPPED: Report results for UNMAPPED proteins
+		INSTANCES: Use Expected Number of Ion Instances to adjust the peptide probabilities prior to NSP adjustment
+		DELUDE: do NOT use peptide degeneracy information when assessing proteins
+		
+		MINPROB: peptideProphet probabilty threshold (default=0.05) 
+		MININDEP: minimum percentage of independent peptides required for a protein (default=0) 
+		
+		
+-->
+
+  <help>
+	Run Peptide Prophet
+  </help>
+
+</tool>