comparison interprophet.xml @ 2:25261529840c

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<tool id="proteomics_search_interprophet_1" name="InterProphet" version="1.0.0">
	
	<requirements>
	    <requirement type="package" version="1.1.9">galaxy_protk</requirement>
	    <requirement type="package" version="4.6.1">trans_proteomic_pipeline</requirement>
   </requirements>


  <description>Combine Peptide Prophet results from multiple search engines</description>

  <command interpreter="ruby">

	interprophet_wrapper.rb $output $use_nss $use_nrs $use_nse $use_nsi $use_nsm --minprob $minprob


	## Inputs.
	${first_input}
	#for $input_file in $input_files:
	${input_file.additional_input}
	#end for  	

  </command>

  <inputs>

	<param name="first_input" type="data" format="peptideprophet_pepxml" label="Peptide Prophet Results" help="These files will typically be outputs from search tools that have subsequently been run through peptide prophet"/> 	
		
	<repeat name="input_files" title="Additional PepXML Input Files">
		<param format="peptideprophet_pepxml" name="additional_input" type="data" label="PepXML produced by Peptide Prophet" help=""/>
	</repeat>
	
	<param name="use_nss" checked="true" type="boolean" label="Include NSS in Model" help="Include NSS (Number of Sibling Searches) in Statistical Model" truevalue="blank" falsevalue="--nonss"/>
	<param name="use_nrs" checked="true" type="boolean" label="Include NRS in Model" help="Include NRS (Number of Replicate Spectra) in Statistical Model" truevalue="blank" falsevalue="--nonrs"/>
	<param name="use_nse" checked="true" type="boolean" label="Include NSE in Model" help="Include NSE (Number of Sibling Experiments) in Statistical Model" truevalue="blank" falsevalue="--nonse"/>
	<param name="use_nsi" checked="true" type="boolean" label="Include NSI in Model" help="Include NSI (Number of Sibling Ions) in Statistical Model" truevalue="blank" falsevalue="--nonsi"/>
	<param name="use_nsm" checked="true" type="boolean" label="Include NSM in Model" help="Include NSM (Number of Sibling Modifications) in Statistical Model" truevalue="blank" falsevalue="--nonsm"/>
	
	<param name="minprob" type="text" label="Minimum threshod probability for reporting results"/>
		
  </inputs>
  <outputs>
    <data format="interprophet_pepxml" name="output" metadata_source="first_input" label="interprophet.${first_input.display_name}" from_work_dir="interprophet_output.pep.xml"/>
  </outputs>

 <help>

**What it does**

Takes a set of pepXML files (possibly generated using different search engines) and calculates updated identification probabilities for each peptide.  The updated probabilities are based on a statistical model that combines evidence from identifications across all of the input files, spectra, modified states and charge states. 

----

**Citation**

If you use this tool please read and cite the paper describing iProphet

Shteynberg D, et al. “iProphet: Improved statistical validation of peptide identifications in shotgun proteomics.” *Molecular and Cellular Proteomics* 10, M111.007690 (2011).

  </help>

</tool>