changeset 10:8f1b150a2487 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/abyss commit 4ad8aac500f821c9ad069e5e8723a0d8af4d2700"
author iuc
date Wed, 12 Jan 2022 17:04:56 +0000
parents 890b794c997d
children 7547fcab5d19
files abyss-pe.xml test-data/assembly_sample-R1.fasta test-data/assembly_sample-R2.fasta
diffstat 3 files changed, 665 insertions(+), 33 deletions(-) [+]
line wrap: on
line diff
--- a/abyss-pe.xml	Tue Jan 04 09:57:32 2022 +0000
+++ b/abyss-pe.xml	Wed Jan 12 17:04:56 2022 +0000
@@ -1,10 +1,11 @@
-<tool id="abyss-pe" name="ABySS" version="@TOOL_VERSION@+galaxy0">
+<tool id="abyss-pe" name="ABySS" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@">
     <description>de novo sequence assembler</description>
     <xrefs>
         <xref type="bio.tools">abyss</xref>
     </xrefs>
     <macros>
         <token name="@TOOL_VERSION@">2.3.4</token>
+        <token name="@VERSION_SUFFIX@">1</token>
         <xml name="reads_conditional">
             <conditional name="reads">
                 <param name="reads_selector" type="select" label="Type of paired-end datasets">
@@ -38,6 +39,49 @@
     </stdio>
     <version_command>ABYSS --version | head -n 1</version_command>
     <command><![CDATA[
+
+#def get_link_fname($data, $prefix, $index)
+    ## Make a name for symlinked files including extension
+    #return ''.join([str($prefix), '_', str($index), '.', str($data.ext)])
+#end def
+
+## Go through all inputs and create symbolic links
+#for $i, $v in enumerate($lib_repeat)
+    #if $v.reads.reads_selector == 'paired'
+        ln -s $v.reads.reads1 $get_link_fname($v.reads.reads1, 'lib_reads1', $i) &&
+        ln -s $v.reads.reads2 $get_link_fname($v.reads.reads2, 'lib_reads2', $i) &&
+    #elif $v.reads.reads_selector == 'paired_collection'
+        ln -s $v.reads.reads_coll.forward $get_link_fname($v.reads.reads_coll.forward, 'lib_reads_coll_forward', $i) &&
+        ln -s $v.reads.reads_coll.reverse $get_link_fname($v.reads.reads_coll.reverse, 'lib_reads_coll_reverse', $i) &&
+    #else
+        ln -s $v.reads.reads_il $get_link_fname($v.reads.reads_il, 'lib_reads_il', $i) &&
+    #end if
+#end for
+
+#for $i, $v in enumerate($mp_repeat)
+    #if $v.reads.reads_selector == 'paired'
+        ln -s $v.reads.reads1 $get_link_fname($v.reads.reads1, 'mp_reads1', $i) &&
+        ln -s $v.reads.reads2 $get_link_fname($v.reads.reads2, 'mp_reads2', $i) &&
+    #elif $v.reads.reads_selector == 'paired_collection'
+        ln -s $v.reads.reads_coll.forward $get_link_fname($v.reads.reads_coll.forward, 'mp_reads_coll_forward', $i) &&
+        ln -s $v.reads.reads_coll.reverse $get_link_fname($v.reads.reads_coll.reverse, 'mp_reads_coll_reverse', $i) &&
+    #else
+        ln -s $v.reads.reads_il $get_link_fname($v.reads.reads_il, 'mp_reads_il', $i) &&
+    #end if
+#end for
+
+#if str($se_reads) != 'None'
+    #for $i, $v in enumerate($se_reads)
+        ln -s $v $get_link_fname($v, 'se_reads', $i) &&
+    #end for
+#end if
+
+#if str($long_seqs) != 'None'
+    #for $i, $v in enumerate($long_seqs)
+        ln -s $v $get_link_fname($v, 'long_seqs', $i) &&
+    #end for
+#end if
+
 abyss-pe
 name=abyss
 j=\${GALAXY_SLOTS:-1}
@@ -99,52 +143,36 @@
     N='$N'
 #end if
 #if $lib_repeat
-    lib='
-    #for $i in range(len($lib_repeat))
-        lib$i
-    #end for
-    '
+    lib='${' '.join(['lib'+str($i) for $i in range(len($lib_repeat))])}'
     #for $i, $v in enumerate($lib_repeat)
         #if $v.reads.reads_selector == 'paired'
-            lib${i}='${v.reads.reads1} ${v.reads.reads2}'
+            lib${i}='${get_link_fname($v.reads.reads1, "lib_reads1", $i)} ${get_link_fname($v.reads.reads2, "lib_reads2", $i)}'
         #elif $v.reads.reads_selector == 'paired_collection'
-            lib${i}='${v.reads.reads_coll.forward} ${v.reads.reads_coll.reverse}'
+            lib${i}='$get_link_fname($v.reads.reads_coll.forward, "lib_reads_coll_forward", $i)} ${get_link_fname($v.reads.reads_coll.reverse, "lib_reads_coll_reverse", $i)}'
         #else
-            lib${i}='${v.reads.reads_il}'
+            lib${i}='${get_link_fname($v.reads.reads_il, "lib_reads_il", $i)}'
         #end if
     #end for
 #end if
 #if str($se_reads) != 'None'
-    se='
-    #for $v in $se_reads
-        $v
-    #end for
-    '
+    se='${' '.join([$get_link_fname($v, "se_reads", $i) for $i, $v in enumerate($se_reads)])}'
 #end if
 #if $mp_repeat
-    mp='
-    #for $i in range(len($mp_repeat))
-        mp$i
-    #end for
-    '
+    mp='${' '.join(['mp'+str($i) for $i in range(len($mp_repeat))])}'
     #for $i, $v in enumerate($mp_repeat)
         #if $v.reads.reads_selector == 'paired'
-            mp${i}='${v.reads.reads1} ${v.reads.reads2}'
+            mp${i}='${get_link_fname($v.reads.reads1, "mp_reads1", $i)} ${get_link_fname($v.reads.reads2, "mp_reads2", $i)}'
         #elif $v.reads.reads_selector == 'paired_collection'
-            mp${i}='${v.reads.reads_coll.forward} ${v.reads.reads_coll.reverse}'
+            mp${i}='$get_link_fname($v.reads.reads_coll.forward, "mp_reads_coll_forward", $i)} ${get_link_fname($v.reads.reads_coll.reverse, "mp_reads_coll_reverse", $i)}'
         #else
-            mp${i}='${v.reads.reads_il}'
+            mp${i}='${get_link_fname($v.reads.reads_il, "mp_reads_il", $i)}'
         #end if
     #end for
 #end if
 #if str($long_seqs) != 'None'
-    long='
-    #for $i in range(len($long_seqs))
-        long$i
-    #end for
-    '
+    long='${' '.join(['long'+str($i) for $i in range(len($long_seqs))])}'
     #for $i, $v in enumerate($long_seqs)
-        long${i}='$v'
+        long$i='${get_link_fname($v, "long_seqs", $i)}'
     #end for
 #end if
     ]]></command>
@@ -203,8 +231,8 @@
             <repeat name="lib_repeat">
                 <conditional name="reads">
                     <param name="reads_selector" value="paired" />
-                    <param name="reads1" ftype="fastqsanger" value="assembly_sample-R1.fastq.gz" />
-                    <param name="reads2" ftype="fastqsanger" value="assembly_sample-R2.fastq.gz" />
+                    <param name="reads1" ftype="fastqsanger.gz" value="assembly_sample-R1.fastq.gz" />
+                    <param name="reads2" ftype="fastqsanger.gz" value="assembly_sample-R2.fastq.gz" />
                 </conditional>
             </repeat>
             <param name="k" value="50" />
@@ -215,15 +243,15 @@
             <output name="stats" file="abyss-stats1.tab" />
         </test>
         <test>
-            <param name="se_reads" ftype="fastqsanger" value="assembly_sample-R1.fastq.gz,assembly_sample-R2.fastq.gz" />
+            <param name="se_reads" ftype="fastq.gz" value="assembly_sample-R1.fastq.gz,assembly_sample-R2.fastq.gz" />
             <param name="k" value="50" />
             <output name="unitigs" file="abyss-unitigs2.fa" />
             <output name="indels" file="empty_file.fasta" />
             <output name="stats" file="abyss-stats2.tab" />
         </test>
         <test>
-            <param name="se_reads" ftype="fastqsanger" value="assembly_sample-R1.fastq.gz,assembly_sample-R2.fastq.gz" />
-            <param name="long_seqs" ftype="fastqsanger" value="assembly_sample-R2.fastq.gz" />
+            <param name="se_reads" ftype="fasta" value="assembly_sample-R1.fasta,assembly_sample-R2.fasta" />
+            <param name="long_seqs" ftype="fasta" value="assembly_sample-R2.fasta" />
             <param name="k" value="50" />
             <output name="unitigs" file="abyss-unitigs3.fa" />
             <output name="long_scaffolds" file="abyss-long-scaffolds3.fa" />
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/assembly_sample-R1.fasta	Wed Jan 12 17:04:56 2022 +0000
@@ -0,0 +1,302 @@
+>HWI-ST745_0097:7:1101:1001:1000#0/1
+GAAAAACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTT
+>HWI-ST745_0097:7:1101:1002:1000#0/1
+AAAAACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTA
+>HWI-ST745_0097:7:1101:1003:1000#0/1
+AAAACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAG
+>HWI-ST745_0097:7:1101:1004:1000#0/1
+AAACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGT
+>HWI-ST745_0097:7:1101:1005:1000#0/1
+AACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTG
+>HWI-ST745_0097:7:1101:1006:1000#0/1
+ACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGG
+>HWI-ST745_0097:7:1101:1007:1000#0/1
+CGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGT
+>HWI-ST745_0097:7:1101:1008:1000#0/1
+GCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTG
+>HWI-ST745_0097:7:1101:1009:1000#0/1
+CGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGA
+>HWI-ST745_0097:7:1101:1010:1000#0/1
+GCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAG
+>HWI-ST745_0097:7:1101:1011:1000#0/1
+CTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGT
+>HWI-ST745_0097:7:1101:1012:1000#0/1
+TTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTC
+>HWI-ST745_0097:7:1101:1013:1000#0/1
+TGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCG
+>HWI-ST745_0097:7:1101:1014:1000#0/1
+GCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGT
+>HWI-ST745_0097:7:1101:1015:1000#0/1
+CAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTC
+>HWI-ST745_0097:7:1101:1016:1000#0/1
+AGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCG
+>HWI-ST745_0097:7:1101:1017:1000#0/1
+GATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGG
+>HWI-ST745_0097:7:1101:1018:1000#0/1
+ATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGT
+>HWI-ST745_0097:7:1101:1019:1000#0/1
+TGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTA
+>HWI-ST745_0097:7:1101:1020:1000#0/1
+GGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTAT
+>HWI-ST745_0097:7:1101:1021:1000#0/1
+GGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATA
+>HWI-ST745_0097:7:1101:1022:1000#0/1
+GTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATAT
+>HWI-ST745_0097:7:1101:1023:1000#0/1
+TCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATC
+>HWI-ST745_0097:7:1101:1024:1000#0/1
+CGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCT
+>HWI-ST745_0097:7:1101:1025:1000#0/1
+GCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTG
+>HWI-ST745_0097:7:1101:1026:1000#0/1
+CAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGA
+>HWI-ST745_0097:7:1101:1027:1000#0/1
+AGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAA
+>HWI-ST745_0097:7:1101:1028:1000#0/1
+GGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAAT
+>HWI-ST745_0097:7:1101:1029:1000#0/1
+GTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATG
+>HWI-ST745_0097:7:1101:1030:1000#0/1
+TTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGG
+>HWI-ST745_0097:7:1101:1031:1000#0/1
+TACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGA
+>HWI-ST745_0097:7:1101:1032:1000#0/1
+ACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAG
+>HWI-ST745_0097:7:1101:1033:1000#0/1
+CTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGA
+>HWI-ST745_0097:7:1101:1034:1000#0/1
+TTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGAT
+>HWI-ST745_0097:7:1101:1035:1000#0/1
+TCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATA
+>HWI-ST745_0097:7:1101:1036:1000#0/1
+CCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATAC
+>HWI-ST745_0097:7:1101:1037:1000#0/1
+CTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACG
+>HWI-ST745_0097:7:1101:1038:1000#0/1
+TTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGA
+>HWI-ST745_0097:7:1101:1039:1000#0/1
+TGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGAC
+>HWI-ST745_0097:7:1101:1040:1000#0/1
+GCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACA
+>HWI-ST745_0097:7:1101:1041:1000#0/1
+CGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAA
+>HWI-ST745_0097:7:1101:1042:1000#0/1
+GCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAAT
+>HWI-ST745_0097:7:1101:1043:1000#0/1
+AGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTA
+>HWI-ST745_0097:7:1101:1044:1000#0/1
+GTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTAT
+>HWI-ST745_0097:7:1101:1045:1000#0/1
+TGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATT
+>HWI-ST745_0097:7:1101:1046:1000#0/1
+GGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTC
+>HWI-ST745_0097:7:1101:1047:1000#0/1
+GTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCG
+>HWI-ST745_0097:7:1101:1048:1000#0/1
+TGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGG
+>HWI-ST745_0097:7:1101:1049:1000#0/1
+AGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGT
+>HWI-ST745_0097:7:1101:1050:1000#0/1
+GTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTA
+>HWI-ST745_0097:7:1101:1051:1000#0/1
+TCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTAC
+>HWI-ST745_0097:7:1101:1052:1000#0/1
+CGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACA
+>HWI-ST745_0097:7:1101:1053:1000#0/1
+GTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAG
+>HWI-ST745_0097:7:1101:1054:1000#0/1
+TCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGG
+>HWI-ST745_0097:7:1101:1055:1000#0/1
+CGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGA
+>HWI-ST745_0097:7:1101:1056:1000#0/1
+GGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGAT
+>HWI-ST745_0097:7:1101:1057:1000#0/1
+GTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATA
+>HWI-ST745_0097:7:1101:1058:1000#0/1
+TATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAA
+>HWI-ST745_0097:7:1101:1059:1000#0/1
+ATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAA
+>HWI-ST745_0097:7:1101:1060:1000#0/1
+ATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGC
+>HWI-ST745_0097:7:1101:1061:1000#0/1
+GGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACA
+>HWI-ST745_0097:7:1101:1062:1000#0/1
+GGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACAT
+>HWI-ST745_0097:7:1101:1063:1000#0/1
+TACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCA
+>HWI-ST745_0097:7:1101:1064:1000#0/1
+ACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCAT
+>HWI-ST745_0097:7:1101:1065:1000#0/1
+CAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATG
+>HWI-ST745_0097:7:1101:1066:1000#0/1
+AGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGG
+>HWI-ST745_0097:7:1101:1067:1000#0/1
+GGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGG
+>HWI-ST745_0097:7:1101:1068:1000#0/1
+ATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGAC
+>HWI-ST745_0097:7:1101:1069:1000#0/1
+GCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAA
+>HWI-ST745_0097:7:1101:1070:1000#0/1
+CTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAAC
+>HWI-ST745_0097:7:1101:1071:1000#0/1
+TGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTAT
+>HWI-ST745_0097:7:1101:1072:1000#0/1
+ATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTA
+>HWI-ST745_0097:7:1101:1073:1000#0/1
+GTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTAC
+>HWI-ST745_0097:7:1101:1074:1000#0/1
+CATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGC
+>HWI-ST745_0097:7:1101:1075:1000#0/1
+CATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGC
+>HWI-ST745_0097:7:1101:1076:1000#0/1
+TGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCG
+>HWI-ST745_0097:7:1101:1077:1000#0/1
+GACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAG
+>HWI-ST745_0097:7:1101:1078:1000#0/1
+ACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGA
+>HWI-ST745_0097:7:1101:1079:1000#0/1
+CAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGAT
+>HWI-ST745_0097:7:1101:1080:1000#0/1
+AAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATA
+>HWI-ST745_0097:7:1101:1081:1000#0/1
+TGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCT
+>HWI-ST745_0097:7:1101:1082:1000#0/1
+GCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTC
+>HWI-ST745_0097:7:1101:1083:1000#0/1
+CCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCT
+>HWI-ST745_0097:7:1101:1084:1000#0/1
+CGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTG
+>HWI-ST745_0097:7:1101:1085:1000#0/1
+GCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGC
+>HWI-ST745_0097:7:1101:1086:1000#0/1
+CAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCA
+>HWI-ST745_0097:7:1101:1087:1000#0/1
+AGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAA
+>HWI-ST745_0097:7:1101:1088:1000#0/1
+ATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGG
+>HWI-ST745_0097:7:1101:1089:1000#0/1
+ATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTT
+>HWI-ST745_0097:7:1101:1090:1000#0/1
+TTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTC
+>HWI-ST745_0097:7:1101:1091:1000#0/1
+TCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCT
+>HWI-ST745_0097:7:1101:1092:1000#0/1
+GTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGC
+>HWI-ST745_0097:7:1101:1093:1000#0/1
+TGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCT
+>HWI-ST745_0097:7:1101:1094:1000#0/1
+GAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCG
+>HWI-ST745_0097:7:1101:1095:1000#0/1
+ACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCG
+>HWI-ST745_0097:7:1101:1096:1000#0/1
+GCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTT
+>HWI-ST745_0097:7:1101:1097:1000#0/1
+CGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATG
+>HWI-ST745_0097:7:1101:1098:1000#0/1
+GATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGAT
+>HWI-ST745_0097:7:1101:1099:1000#0/1
+TCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGT
+>HWI-ST745_0097:7:1101:1100:1000#0/1
+TATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTC
+>HWI-ST745_0097:7:1101:1101:1000#0/1
+TAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCAC
+>HWI-ST745_0097:7:1101:1102:1000#0/1
+CTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTT
+>HWI-ST745_0097:7:1101:1103:1000#0/1
+CTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTT
+>HWI-ST745_0097:7:1101:1104:1000#0/1
+AACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCG
+>HWI-ST745_0097:7:1101:1105:1000#0/1
+CCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTT
+>HWI-ST745_0097:7:1101:1106:1000#0/1
+CTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCAC
+>HWI-ST745_0097:7:1101:1107:1000#0/1
+TGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACG
+>HWI-ST745_0097:7:1101:1108:1000#0/1
+GCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGA
+>HWI-ST745_0097:7:1101:1109:1000#0/1
+CAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAA
+>HWI-ST745_0097:7:1101:1110:1000#0/1
+AAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAG
+>HWI-ST745_0097:7:1101:1111:1000#0/1
+AGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGC
+>HWI-ST745_0097:7:1101:1112:1000#0/1
+GGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCT
+>HWI-ST745_0097:7:1101:1113:1000#0/1
+GTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTC
+>HWI-ST745_0097:7:1101:1114:1000#0/1
+TTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCT
+>HWI-ST745_0097:7:1101:1115:1000#0/1
+TCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTC
+>HWI-ST745_0097:7:1101:1116:1000#0/1
+TAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCG
+>HWI-ST745_0097:7:1101:1117:1000#0/1
+AGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGG
+>HWI-ST745_0097:7:1101:1118:1000#0/1
+ACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCA
+>HWI-ST745_0097:7:1101:1119:1000#0/1
+ATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCA
+>HWI-ST745_0097:7:1101:1120:1000#0/1
+ATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCA
+>HWI-ST745_0097:7:1101:1121:1000#0/1
+GTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACA
+>HWI-ST745_0097:7:1101:1122:1000#0/1
+TTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACAT
+>HWI-ST745_0097:7:1101:1123:1000#0/1
+TTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACAT
+>HWI-ST745_0097:7:1101:1124:1000#0/1
+TTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACAT
+>HWI-ST745_0097:7:1101:1125:1000#0/1
+CACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACATCCGG
+>HWI-ST745_0097:7:1101:1126:1000#0/1
+AGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAA
+>HWI-ST745_0097:7:1101:1127:1000#0/1
+AGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAA
+>HWI-ST745_0097:7:1101:1128:1000#0/1
+CTCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAACT
+>HWI-ST745_0097:7:1101:1129:1000#0/1
+TCTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAACTG
+>HWI-ST745_0097:7:1101:1130:1000#0/1
+CTCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAACTGG
+>HWI-ST745_0097:7:1101:1131:1000#0/1
+TCTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAACTGGC
+>HWI-ST745_0097:7:1101:1132:1000#0/1
+CTGCTATTCCGTTACTCTCCGGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAACTGGCG
+>HWI-ST745_0097:7:1101:1133:1000#0/1
+GGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAACTGGCGTGTTTCATTAGCCCGGTAGC
+>HWI-ST745_0097:7:1101:1134:1000#0/1
+GGACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAACTGGCGTGTTTCATTAGCCCGGTAGC
+>HWI-ST745_0097:7:1101:1135:1000#0/1
+GACTCCGCACCGCCGTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAACTGGCGTGTTTCATTAGCCCGGTAGCA
+>HWI-ST745_0097:7:1101:1136:1000#0/1
+CCGTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAACTGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATC
+>HWI-ST745_0097:7:1101:1137:1000#0/1
+GTGTTCTTCGGTTCAAGTCCCAACATCCGGGCGAACTGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATCCG
+>HWI-ST745_0097:7:1101:1138:1000#0/1
+TTCTTCGGTTCAAGTCCCAACATCCGGGCGAACTGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATCCGTCA
+>HWI-ST745_0097:7:1101:1139:1000#0/1
+GGTTCAAGTCCCAACATCCGGGCGAACTGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATCCGTCAGCCACT
+>HWI-ST745_0097:7:1101:1140:1000#0/1
+TTCAAGTCCCAACATCCGGGCGAACTGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATCCGTCAGCCACTCC
+>HWI-ST745_0097:7:1101:1142:1000#0/1
+CCGGGCGAACTGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATCCGTCAGCCACTCCACTGGAGACGACGGA
+>HWI-ST745_0097:7:1101:1141:1000#0/1
+CGGGCGAACTGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATCCGTCAGCCACTCCACTGGAGACGACGGAA
+>HWI-ST745_0097:7:1101:1145:1000#0/1
+AACTGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATCCGTCAGCCACTCCACTGGAGACGACGGAAGATCGT
+>HWI-ST745_0097:7:1101:1143:1000#0/1
+ACTGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATCCGTCAGCCACTCCACTGGAGACGACGGAAGATCGTT
+>HWI-ST745_0097:7:1101:1144:1000#0/1
+CTGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATCCGTCAGCCACTCCACTGGAGACGACGGAAGATCGTTG
+>HWI-ST745_0097:7:1101:1146:1000#0/1
+CTGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATCCGTCAGCCACTCCACTGGAGACGACGGAAGATCGTTG
+>HWI-ST745_0097:7:1101:1147:1000#0/1
+TGGCGTGTTTCATTAGCCCGGTAGCATGAACCATTATCCGTCAGCCACTCCACTGGAGACGACGGAAGATCGTTGC
+>HWI-ST745_0097:7:1101:1148:1000#0/1
+CCGGTAGCATGAACCATTATCCGTCAGCCACTCCACTGGAGACGACGGAAGATCGTTGCCGAAGCGGCGTTCCACC
+>HWI-ST745_0097:7:1101:1149:1000#0/1
+CATGAACCATTATCCGTCAGCCACTCCACTGGAGACGACGGAAGATCGTTGCCGAAGCGGCGTTCCACCGCTCCCA
+>HWI-ST745_0097:7:1101:1150:1000#0/1
+ATGAACCATTATCCGTCAGCCACTCCACTGGAGACGACGGAAGATCGTTGCCGAAGCGGCGTTCCACCGCTCCCAG
+>HWI-ST745_0097:7:1101:1151:1000#0/1
+TGAACCATTATCCGTCAGCCACTCCACTGGAGACGACGGAAGATCGTTGCCGAAGCGGCGTTCCACCGCTCCCAGC
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/assembly_sample-R2.fasta	Wed Jan 12 17:04:56 2022 +0000
@@ -0,0 +1,302 @@
+>HWI-ST745_0097:7:1101:1001:1000#0/2
+GCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGGGAATTCTCCGCTGATTGTC
+>HWI-ST745_0097:7:1101:1002:1000#0/2
+TTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGGGAA
+>HWI-ST745_0097:7:1101:1003:1000#0/2
+ATGTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAG
+>HWI-ST745_0097:7:1101:1004:1000#0/2
+CAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGC
+>HWI-ST745_0097:7:1101:1005:1000#0/2
+CCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCC
+>HWI-ST745_0097:7:1101:1006:1000#0/2
+CCCGAATAACCCCGGCAAACCCCAGAGGGAATTCTCCGCTGATTGTCGTATCTCCATTCAGATATACCGACGACTC
+>HWI-ST745_0097:7:1101:1007:1000#0/2
+AAATATAGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGC
+>HWI-ST745_0097:7:1101:1008:1000#0/2
+TGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGGGAATTCTCCGCTGATTGT
+>HWI-ST745_0097:7:1101:1009:1000#0/2
+GCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGGGAATTCTCCGCTGATTGTC
+>HWI-ST745_0097:7:1101:1010:1000#0/2
+GGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGG
+>HWI-ST745_0097:7:1101:1011:1000#0/2
+TTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGG
+>HWI-ST745_0097:7:1101:1012:1000#0/2
+CTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGGGAATTCTCCGCTGATTGTCGTATCTCCATTCAGATA
+>HWI-ST745_0097:7:1101:1013:1000#0/2
+CAGGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGC
+>HWI-ST745_0097:7:1101:1014:1000#0/2
+CCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGGGAATTCTCCGCTGA
+>HWI-ST745_0097:7:1101:1015:1000#0/2
+CTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTA
+>HWI-ST745_0097:7:1101:1016:1000#0/2
+GTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCG
+>HWI-ST745_0097:7:1101:1017:1000#0/2
+CATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGA
+>HWI-ST745_0097:7:1101:1018:1000#0/2
+AGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGC
+>HWI-ST745_0097:7:1101:1019:1000#0/2
+CAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCCATGA
+>HWI-ST745_0097:7:1101:1020:1000#0/2
+AGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGGGAATTCTCCGCTGATTGTCGTATCTCCATTCAGA
+>HWI-ST745_0097:7:1101:1021:1000#0/2
+TGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCC
+>HWI-ST745_0097:7:1101:1022:1000#0/2
+GTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCG
+>HWI-ST745_0097:7:1101:1023:1000#0/2
+CAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGGGAATTCTCCGCTGATTGTCGTATCTCCATTC
+>HWI-ST745_0097:7:1101:1024:1000#0/2
+CATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGAT
+>HWI-ST745_0097:7:1101:1025:1000#0/2
+AGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGGGAATTCTCCGCTGATTGTCGTATCTCCAT
+>HWI-ST745_0097:7:1101:1026:1000#0/2
+GATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTAC
+>HWI-ST745_0097:7:1101:1027:1000#0/2
+CCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGGGAATTCTCCGCTGATTGTCGTATCTCC
+>HWI-ST745_0097:7:1101:1028:1000#0/2
+AGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCCATGAT
+>HWI-ST745_0097:7:1101:1029:1000#0/2
+TAACAGATGTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCAT
+>HWI-ST745_0097:7:1101:1030:1000#0/2
+ATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGT
+>HWI-ST745_0097:7:1101:1031:1000#0/2
+AGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCC
+>HWI-ST745_0097:7:1101:1032:1000#0/2
+GCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATC
+>HWI-ST745_0097:7:1101:1033:1000#0/2
+TCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAA
+>HWI-ST745_0097:7:1101:1034:1000#0/2
+TCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACC
+>HWI-ST745_0097:7:1101:1035:1000#0/2
+CGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT
+>HWI-ST745_0097:7:1101:1036:1000#0/2
+AATATAGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCC
+>HWI-ST745_0097:7:1101:1037:1000#0/2
+TTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGA
+>HWI-ST745_0097:7:1101:1038:1000#0/2
+AATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACTG
+>HWI-ST745_0097:7:1101:1039:1000#0/2
+TTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTT
+>HWI-ST745_0097:7:1101:1040:1000#0/2
+GGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAA
+>HWI-ST745_0097:7:1101:1041:1000#0/2
+GCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAA
+>HWI-ST745_0097:7:1101:1042:1000#0/2
+CGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGAATAACCCCGGCAAACCCCAGAGGGAATTCTCCGCT
+>HWI-ST745_0097:7:1101:1043:1000#0/2
+TTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATCCTGTACCCGA
+>HWI-ST745_0097:7:1101:1044:1000#0/2
+GTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTAT
+>HWI-ST745_0097:7:1101:1045:1000#0/2
+GGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCCAT
+>HWI-ST745_0097:7:1101:1046:1000#0/2
+TAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACT
+>HWI-ST745_0097:7:1101:1047:1000#0/2
+GCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCC
+>HWI-ST745_0097:7:1101:1048:1000#0/2
+CGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGG
+>HWI-ST745_0097:7:1101:1049:1000#0/2
+CAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCCCATGA
+>HWI-ST745_0097:7:1101:1050:1000#0/2
+CGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCG
+>HWI-ST745_0097:7:1101:1051:1000#0/2
+TGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTG
+>HWI-ST745_0097:7:1101:1052:1000#0/2
+TCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTATC
+>HWI-ST745_0097:7:1101:1053:1000#0/2
+AATATAGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCC
+>HWI-ST745_0097:7:1101:1054:1000#0/2
+TGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTTTA
+>HWI-ST745_0097:7:1101:1055:1000#0/2
+ACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTT
+>HWI-ST745_0097:7:1101:1056:1000#0/2
+CTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCTT
+>HWI-ST745_0097:7:1101:1057:1000#0/2
+TCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCACTGCCAATTTCCAGCAAAGCT
+>HWI-ST745_0097:7:1101:1058:1000#0/2
+CGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAG
+>HWI-ST745_0097:7:1101:1059:1000#0/2
+ATATAGGGGCAAATCCAGGGTTCTTGTCCCATGATGTTCAAAACTGTCAATATCCTGCATTGTTAGCGTAGCGCCA
+>HWI-ST745_0097:7:1101:1060:1000#0/2
+TGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGGTTCTTGTCC
+>HWI-ST745_0097:7:1101:1061:1000#0/2
+CATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTA
+>HWI-ST745_0097:7:1101:1062:1000#0/2
+TTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATC
+>HWI-ST745_0097:7:1101:1063:1000#0/2
+AGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGG
+>HWI-ST745_0097:7:1101:1064:1000#0/2
+CATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATA
+>HWI-ST745_0097:7:1101:1065:1000#0/2
+CATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTA
+>HWI-ST745_0097:7:1101:1066:1000#0/2
+ACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATA
+>HWI-ST745_0097:7:1101:1067:1000#0/2
+AGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTG
+>HWI-ST745_0097:7:1101:1068:1000#0/2
+ATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGGTTCT
+>HWI-ST745_0097:7:1101:1069:1000#0/2
+CAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAA
+>HWI-ST745_0097:7:1101:1070:1000#0/2
+TATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGG
+>HWI-ST745_0097:7:1101:1071:1000#0/2
+ATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGC
+>HWI-ST745_0097:7:1101:1072:1000#0/2
+TAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGG
+>HWI-ST745_0097:7:1101:1073:1000#0/2
+CGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCT
+>HWI-ST745_0097:7:1101:1074:1000#0/2
+AGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGT
+>HWI-ST745_0097:7:1101:1075:1000#0/2
+CTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATC
+>HWI-ST745_0097:7:1101:1076:1000#0/2
+CATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAA
+>HWI-ST745_0097:7:1101:1077:1000#0/2
+GATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTC
+>HWI-ST745_0097:7:1101:1078:1000#0/2
+CGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTC
+>HWI-ST745_0097:7:1101:1079:1000#0/2
+GGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGAC
+>HWI-ST745_0097:7:1101:1080:1000#0/2
+CGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCAT
+>HWI-ST745_0097:7:1101:1081:1000#0/2
+CGCCGCTTCGGCAACGATCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATG
+>HWI-ST745_0097:7:1101:1082:1000#0/2
+TCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGC
+>HWI-ST745_0097:7:1101:1083:1000#0/2
+CGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGA
+>HWI-ST745_0097:7:1101:1084:1000#0/2
+GGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATC
+>HWI-ST745_0097:7:1101:1085:1000#0/2
+GGCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGT
+>HWI-ST745_0097:7:1101:1086:1000#0/2
+GGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGAC
+>HWI-ST745_0097:7:1101:1087:1000#0/2
+CCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGA
+>HWI-ST745_0097:7:1101:1088:1000#0/2
+CACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGT
+>HWI-ST745_0097:7:1101:1089:1000#0/2
+TCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGA
+>HWI-ST745_0097:7:1101:1090:1000#0/2
+GCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAA
+>HWI-ST745_0097:7:1101:1091:1000#0/2
+GTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACC
+>HWI-ST745_0097:7:1101:1092:1000#0/2
+GAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTAC
+>HWI-ST745_0097:7:1101:1093:1000#0/2
+TCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTT
+>HWI-ST745_0097:7:1101:1094:1000#0/2
+CCGCTTCGGCAACGATCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAA
+>HWI-ST745_0097:7:1101:1095:1000#0/2
+ACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTA
+>HWI-ST745_0097:7:1101:1096:1000#0/2
+GAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATC
+>HWI-ST745_0097:7:1101:1097:1000#0/2
+CTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGC
+>HWI-ST745_0097:7:1101:1098:1000#0/2
+GCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTA
+>HWI-ST745_0097:7:1101:1099:1000#0/2
+TACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCTT
+>HWI-ST745_0097:7:1101:1100:1000#0/2
+TAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCG
+>HWI-ST745_0097:7:1101:1101:1000#0/2
+CTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTC
+>HWI-ST745_0097:7:1101:1102:1000#0/2
+TCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGT
+>HWI-ST745_0097:7:1101:1103:1000#0/2
+GTGGAACGCCGCTTCGGCAACGATCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGG
+>HWI-ST745_0097:7:1101:1104:1000#0/2
+GATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAA
+>HWI-ST745_0097:7:1101:1105:1000#0/2
+GCCGCTTCGGCAACGATCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGA
+>HWI-ST745_0097:7:1101:1106:1000#0/2
+CTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCG
+>HWI-ST745_0097:7:1101:1107:1000#0/2
+CCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGAC
+>HWI-ST745_0097:7:1101:1108:1000#0/2
+GTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGC
+>HWI-ST745_0097:7:1101:1109:1000#0/2
+CGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAA
+>HWI-ST745_0097:7:1101:1110:1000#0/2
+GAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGACTTGAAC
+>HWI-ST745_0097:7:1101:1111:1000#0/2
+GACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAAC
+>HWI-ST745_0097:7:1101:1112:1000#0/2
+CTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCC
+>HWI-ST745_0097:7:1101:1113:1000#0/2
+GGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAA
+>HWI-ST745_0097:7:1101:1114:1000#0/2
+ACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCA
+>HWI-ST745_0097:7:1101:1115:1000#0/2
+GATCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCG
+>HWI-ST745_0097:7:1101:1116:1000#0/2
+CTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCT
+>HWI-ST745_0097:7:1101:1117:1000#0/2
+CGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGT
+>HWI-ST745_0097:7:1101:1118:1000#0/2
+CGTGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTG
+>HWI-ST745_0097:7:1101:1119:1000#0/2
+GTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATG
+>HWI-ST745_0097:7:1101:1120:1000#0/2
+TGATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGA
+>HWI-ST745_0097:7:1101:1121:1000#0/2
+TCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGG
+>HWI-ST745_0097:7:1101:1122:1000#0/2
+CGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAA
+>HWI-ST745_0097:7:1101:1123:1000#0/2
+CTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCTTCCGTCGTCT
+>HWI-ST745_0097:7:1101:1124:1000#0/2
+CTGCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATG
+>HWI-ST745_0097:7:1101:1125:1000#0/2
+CTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCAT
+>HWI-ST745_0097:7:1101:1126:1000#0/2
+CGGTGGAACGCCGCTTCGGCAACGATCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCG
+>HWI-ST745_0097:7:1101:1127:1000#0/2
+ACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGC
+>HWI-ST745_0097:7:1101:1128:1000#0/2
+CGGCAACGATCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGC
+>HWI-ST745_0097:7:1101:1129:1000#0/2
+TCGGCAACGATCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACG
+>HWI-ST745_0097:7:1101:1130:1000#0/2
+CCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCTTCC
+>HWI-ST745_0097:7:1101:1131:1000#0/2
+GTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCTTCCGTCGTCTCCAGTGGAGTGGCTGA
+>HWI-ST745_0097:7:1101:1132:1000#0/2
+AACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCTTCCGTCGTCTCCAG
+>HWI-ST745_0097:7:1101:1133:1000#0/2
+CGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCTTCCG
+>HWI-ST745_0097:7:1101:1134:1000#0/2
+AACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCTTCCGTCGTCTCCAG
+>HWI-ST745_0097:7:1101:1135:1000#0/2
+ACTGCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCAT
+>HWI-ST745_0097:7:1101:1136:1000#0/2
+TTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTAC
+>HWI-ST745_0097:7:1101:1137:1000#0/2
+GTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCTTCCGTCGTCTCCAGTGGA
+>HWI-ST745_0097:7:1101:1138:1000#0/2
+CTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCT
+>HWI-ST745_0097:7:1101:1139:1000#0/2
+GCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCT
+>HWI-ST745_0097:7:1101:1140:1000#0/2
+CGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGG
+>HWI-ST745_0097:7:1101:1142:1000#0/2
+GAGACTGCGTGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTC
+>HWI-ST745_0097:7:1101:1141:1000#0/2
+TGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTG
+>HWI-ST745_0097:7:1101:1145:1000#0/2
+GATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAG
+>HWI-ST745_0097:7:1101:1143:1000#0/2
+AGAGAGACTGCGTGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGC
+>HWI-ST745_0097:7:1101:1144:1000#0/2
+TGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCG
+>HWI-ST745_0097:7:1101:1146:1000#0/2
+TGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAA
+>HWI-ST745_0097:7:1101:1147:1000#0/2
+CTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCC
+>HWI-ST745_0097:7:1101:1148:1000#0/2
+GATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAG
+>HWI-ST745_0097:7:1101:1149:1000#0/2
+CTGCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATG
+>HWI-ST745_0097:7:1101:1150:1000#0/2
+TAACGGAGAGAGACTGCGTGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACC
+>HWI-ST745_0097:7:1101:1151:1000#0/2
+AACGGAGAGAGACTGCGTGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGGGCGGTCACTACCG