Mercurial > repos > iuc > bbtools_callvariants
annotate readme.rst @ 10:7e041c120b96 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit cd59ba2c349865259b92302a1d70e103b8a5e3cb
author | iuc |
---|---|
date | Tue, 27 Aug 2024 10:14:45 +0000 |
parents | ee407fae3ccd |
children |
rev | line source |
---|---|
5
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
1 |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
2 ================= |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
3 IMPORTANT NOTE REGARDING SYSTEM CONFIGURATION |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
4 ================= |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
5 |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
6 All of the Galaxy wrappers contained herein call the respective bbtools' shell wrapper, which calls the underlying java-based tool. Unlike a C-based program, java will grab a pre-determined amount of memory at the very beginning of the execution. |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
7 |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
8 Some of the algorithms (e.g. bbnorm) utilise a hash table, and potential collusions can decrease the numeric accuracy of the output. This problem is expected to become more pronounced if the fraction of the memory occupied w.r.t. allocated memory becomes high, i.e. when the available memory is low and/or the input file is big. If the tool generates a warning to stderr, and will be caught by the Galaxy wrapper resulting in a failed job. However, `count min sketch <https://en.wikipedia.org/wiki/Count%E2%80%93min_sketch>` does not run out of memory, this is a gradual effect, and will NOT trigger a fatal error unless the load reaches this critically high level. You can read more about the implications of this at the `BBtools manual <https://jgi.doe.gov/data-and-tools/software-tools/bbtools/bb-tools-user-guide/bbnorm-guide/>` |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
9 |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
10 If you are administering a heteregenous computing environment with multiple nodes of very different quantities of physically available RAM, it is recommended to define a global cap on the RAM to be used to avoid introducing run-to-run bias by exporting an environmental variable, by something like: |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
11 export _JAVA_OPTIONS="-Xmx2048m -Xms256m" |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
12 |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
13 The tool currently considers the following limits, in the given priority order: |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
14 1) _JAVA_OPTIONS |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
15 2) JAVA_TOOL_OPTIONS |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
16 3) GALAXY_MEMORY_MB |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
17 4) 4 GB |
ee407fae3ccd
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bbtools commit 35db9ac8668f3e376886ea09de63c87dce93e1ce
iuc
parents:
diff
changeset
|
18 |