view getfastaBed.xml @ 24:33c3ddea63c5 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bedtools commit 09721a12bdd393441b162056316241aba3895a4f

<tool id="bedtools_getfastabed" name="GetFastaBed" version="@WRAPPER_VERSION@.1">
    <description>use intervals to extract sequences from a FASTA file</description>
    <macros>
        <import>macros.xml</import>
    </macros>
    <expand macro="requirements" />
    <expand macro="stdio" />
    <command>
<![CDATA[
        #if str( $fasta_source.fasta_source_selector ) == 'history':
          #set $fasta_file = $fasta_source.fasta
        #else
          #set $fasta_file = $fasta_source.fasta_id.fields.path
        #end if
        bedtools getfasta
        $name
        $tab
        $strand
        $split
        -fi '$fasta_file'
        -bed '$input'
        -fo '$output'
]]>
    </command>
    <inputs>
        <param format="bed,vcf,gff,gff3" name="input" type="data" label="BED/VCF/GFF file" />
        <conditional name="fasta_source">
            <param name="fasta_source_selector" type="select" label="Choose the source for the fasta file">
                <option value="history" selected="True">History</option>
                <option value="preloaded">Server indexed files</option>
            </param>
            <when value="history">
                <param name="fasta" format="fasta" type="data" label="Fasta file" />
            </when>
            <when value="preloaded">
               <param name="fasta_id" type="select">
                  <options from_data_table="fasta_indexes" />
               </param>
            </when>
        </conditional>
        <param name="name" type="boolean" checked="false" truevalue="-name" falsevalue=""
            label="Use the 'name' column in the BED file for the FASTA headers in the output FASTA file"
            help="(-name)" />
        <param name="tab" type="boolean" checked="false" truevalue="-tab" falsevalue=""
            label="Report extract sequences in a tab-delimited format instead of in FASTA format"
            help="(-tab)" />
        <param name="strand" type="boolean" checked="false" truevalue="-s" falsevalue=""
            label="Force strandedness"
            help="If the feature occupies the antisense strand, the sequence will be reverse complemented. (-s)" />
        <expand macro="split" />
    </inputs>
    <outputs>
        <data format="fasta" name="output">
            <change_format>
                <when input="tab" value="-tab" format="tabular" />
            </change_format>
        </data>
    </outputs>
    <tests>
        <test>
            <param name="input" value="nucBed1.bed" ftype="bed" />
            <param name="fasta" value="nucBed1.fasta" ftype="fasta" />
            <param name="tab" value="False" />
            <param name="split" value="False" />
            <output name="output" file="getfastaBed_result1.bed" ftype="fasta" />
        </test>
        <test>
            <param name="input" value="nucBed1.bed" ftype="bed" />
            <param name="fasta" value="nucBed1.fasta" ftype="fasta" />
            <param name="tab" value="True" />
            <param name="split" value="False" />
            <output name="output" file="getfastaBed_result2.tabular" ftype="tabular" />
        </test>
    </tests>
    <help>
<![CDATA[
**What it does**

bedtools getfasta will extract the sequence defined by the coordinates in a BED interval and create a new FASTA entry in the output file for each extracted sequence. By default, the FASTA header for each extracted sequence will be formatted as follows: “>chrom>:&lt;start>-&lt;end>”.

.. image:: $PATH_TO_IMAGES/getfasta-glyph.png

.. class:: warningmark

1. The headers in the input FASTA file must exactly match the chromosome column in the BED file.

2. You can use the UNIX fold command to set the line width of the FASTA output. For example, fold -w 60 will make each line of the FASTA file have at most 60 nucleotides for easy viewing.

@REFERENCES@
]]>
    </help>
    <expand macro="citations" />
</tool>