changeset 0:a03e97724306 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/biom_format commit f726b353973996a1f59e2044e80ef7c9b75833ab"
author iuc
date Wed, 11 Sep 2019 12:41:04 -0400
parents
children be44ed503d2b
files biom_normalize_table.xml macros.xml test-data/biom_from_uc.biom test-data/input_abundance_1.biom1 test-data/input_abundance_1.tabular test-data/input_abundance_taxonomy_1.tabular test-data/input_taxonomy_1.tabular test-data/normalized_presence_absence_observations.biom test-data/normalized_relative_abund_samples.biom test-data/observation_subsetting test-data/output_1.biom1.re test-data/output_taxonomy_1.biom1.re test-data/sample_subsetting test-data/subset_observations.biom test-data/subset_samples.biom test-data/summarize test-data/summarize_qualitative_observations test-data/uc_table.uc
diffstat 18 files changed, 1229 insertions(+), 0 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/biom_normalize_table.xml	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,50 @@
+<tool id="biom_normalize_table" name="Normalize" version="@VERSION@.1">
+    <description>a BIOM table</description>
+    <macros>
+        <import>macros.xml</import>
+    </macros>
+    <expand macro="requirements" />
+    <expand macro="version_command" />
+    <command detect_errors="exit_code">
+        <![CDATA[
+            biom normalize-table
+              --input-fp '$input_fp'
+              --output-fp '$output_fp'
+              $relative_abund
+              $presence_absence
+              --axis '$axis'
+        ]]>
+    </command>
+    <inputs>
+        <param name="input_fp" argument="--input-fp" type="data" format="biom1" label="BIOM table to normalize" />
+        <param name="relative_abund" argument="--relative-abund" type="boolean" checked="True" truevalue="--relative-abund" falsevalue="" label="Convert table to relative abundance" />
+        <param name="presence_absence" argument="--presence-absence" type="boolean" checked="True" truevalue="--presence-absence" falsevalue="" label="Convert table to presence/absence" />
+        <param argument="--axis" type="select" label="The axis to normalize over">
+            <option value="sample" selected="True">Sample</option>
+            <option value="observation">Observation</option>
+        </param>
+    </inputs>
+    <outputs>
+        <data format="biom1" name="output_fp"/>
+    </outputs>
+    <tests>
+        <test>
+            <param name="input_fp" value="input_abundance_1.biom1" />
+            <param name="relative_abund" value="--relative-abund" />
+            <param name="presence_absence" value="" />
+            <param name="axis" value="sample" />
+            <output name="output_fp" value="normalized_relative_abund_samples.biom" ftype="biom1" compare="sim_size"/>
+        </test>
+        <test>
+            <param name="input_fp" value="input_abundance_1.biom1" />
+            <param name="relative_abund" value="" />
+            <param name="presence_absence" value="--presence-absence" />
+            <param name="axis" value="observation" />
+            <output name="output_fp" value="normalized_presence_absence_observations.biom" ftype="biom1" compare="sim_size"/>
+        </test>
+    </tests>
+    <help><![CDATA[
+Normalize the values of a BIOM table through various methods. Relative abundance will take the relative abundance of each observation in terms of samples or observations.  Presence absensece will convert observations to 1's and 0's based on presence of the observation.
+    ]]></help>
+    <expand macro="citations" />
+</tool>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/macros.xml	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,18 @@
+<macros>
+    <xml name="requirements">
+        <requirements>
+            <requirement type="package" version="2.1.5">biom-format</requirement>
+            <yield/>
+        </requirements>
+    </xml>
+    <token name="@VERSION@">2.1.5</token>
+    <xml name="version_command">
+        <version_command>biom --version</version_command>
+    </xml>
+    <xml name="citations">
+        <citations>
+            <citation type="doi">10.1186/2047-217X-1-7</citation>
+            <yield/>
+        </citations>
+    </xml>
+</macros>
Binary file test-data/biom_from_uc.biom has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/input_abundance_1.biom1	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,1 @@
+{"id": "None","format": "Biological Observation Matrix 1.0.0","format_url": "http://biom-format.org","matrix_type": "sparse","generated_by": "BIOM-Format 2.1.5","date": "2016-05-26T17:20:40.263567","type": "OTU table","matrix_element_type": "float","shape": [19, 2],"data": [[1,0,160.0],[1,1,242.0],[6,0,1.0],[6,1,1.0],[7,0,3.0],[7,1,4.0],[12,0,13.0],[12,1,36.0],[14,0,1.0],[14,1,5.0],[15,0,1.0],[16,0,1.0],[16,1,3.0]],"rows": [{"id": "2", "metadata": null},{"id": "3", "metadata": null},{"id": "4", "metadata": null},{"id": "5", "metadata": null},{"id": "8", "metadata": null},{"id": "9", "metadata": null},{"id": "10", "metadata": null},{"id": "11", "metadata": null},{"id": "12", "metadata": null},{"id": "14", "metadata": null},{"id": "15", "metadata": null},{"id": "16", "metadata": null},{"id": "17", "metadata": null},{"id": "18", "metadata": null},{"id": "22", "metadata": null},{"id": "23", "metadata": null},{"id": "25", "metadata": null},{"id": "28", "metadata": null},{"id": "29", "metadata": null}],"columns": [{"id": "SAMPLE_1", "metadata": null},{"id": "SAMPLE_2", "metadata": null}]}
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/input_abundance_1.tabular	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,20 @@
+ID	SAMPLE_1	SAMPLE_2
+2	0	0
+3	160	242
+4	0	0
+5	0	0
+8	0	0
+9	0	0
+10	1	1
+11	3	4
+12	0	0
+14	0	0
+15	0	0
+16	0	0
+17	13	36
+18	0	0
+22	1	5
+23	1	0
+25	1	3
+28	0	0
+29	0	0
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/input_abundance_taxonomy_1.tabular	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,20 @@
+#ID	SAMPLE_1	SAMPLE_2	taxonomy
+2	0	0	d__Archaea
+3	160	242	d__Bacteria
+4	0	0	d__Archaea;p__Crenarchaeota
+5	0	0	d__Archaea;p__Euryarchaeota
+8	0	0	d__Bacteria;p__AC1
+9	0	0	d__Bacteria;p__AD3
+10	1	1	d__Bacteria;p__Acidobacteria
+11	3	4	d__Bacteria;p__Actinobacteria
+12	0	0	d__Bacteria;p__AncK6
+14	0	0	d__Bacteria;p__Armatimonadetes
+15	0	0	d__Bacteria;p__BHI80-139
+16	0	0	d__Bacteria;p__BRC1
+17	13	36	d__Bacteria;p__Bacteroidetes
+18	0	0	d__Bacteria;p__CD12
+22	1	5	d__Bacteria;p__Chlorobi
+23	1	0	d__Bacteria;p__Chloroflexi
+25	1	3	d__Bacteria;p__Cyanobacteria
+28	0	0	d__Bacteria;p__EM19
+29	0	0	d__Bacteria;p__EM3
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/input_taxonomy_1.tabular	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,20 @@
+#ID	taxonomy
+2	d__Archaea
+3	d__Bacteria
+4	d__Archaea;p__Crenarchaeota
+5	d__Archaea;p__Euryarchaeota
+8	d__Bacteria;p__AC1
+9	d__Bacteria;p__AD3
+10	d__Bacteria;p__Acidobacteria
+11	d__Bacteria;p__Actinobacteria
+12	d__Bacteria;p__AncK6
+14	d__Bacteria;p__Armatimonadetes
+15	d__Bacteria;p__BHI80-139
+16	d__Bacteria;p__BRC1
+17	d__Bacteria;p__Bacteroidetes
+18	d__Bacteria;p__CD12
+22	d__Bacteria;p__Chlorobi
+23	d__Bacteria;p__Chloroflexi
+25	d__Bacteria;p__Cyanobacteria
+28	d__Bacteria;p__EM19
+29	d__Bacteria;p__EM3
Binary file test-data/normalized_presence_absence_observations.biom has changed
Binary file test-data/normalized_relative_abund_samples.biom has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/observation_subsetting	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,2 @@
+11
+23
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/output_1.biom1.re	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,1 @@
+\{\"id\"\:\ \"None\"\,\"format\"\:\ \"Biological\ Observation\ Matrix\ 1\.0\.0\"\,\"format\_url\"\:\ \"http\:\/\/biom\-format\.org\"\,\"matrix\_type\"\:\ \"sparse\"\,\"generated\_by\"\:\ \"BIOM\-Format\ 2\.1\.5\"\,\"date\"\:\ \".*\"\,\"type\"\:\ \"OTU\ table\"\,\"matrix\_element\_type\"\:\ \"float\"\,\"shape\"\:\ \[19\,\ 2\]\,\"data\"\:\ \[\[1\,0\,160\.0\]\,\[1\,1\,242\.0\]\,\[6\,0\,1\.0\]\,\[6\,1\,1\.0\]\,\[7\,0\,3\.0\]\,\[7\,1\,4\.0\]\,\[12\,0\,13\.0\]\,\[12\,1\,36\.0\]\,\[14\,0\,1\.0\]\,\[14\,1\,5\.0\]\,\[15\,0\,1\.0\]\,\[16\,0\,1\.0\]\,\[16\,1\,3\.0\]\]\,\"rows\"\:\ \[\{\"id\"\:\ \"2\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"3\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"4\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"5\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"8\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"9\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"10\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"11\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"12\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"14\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"15\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"16\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"17\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"18\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"22\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"23\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"25\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"28\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"29\"\,\ \"metadata\"\:\ null\}\]\,\"columns\"\:\ \[\{\"id\"\:\ \"SAMPLE\_1\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"SAMPLE\_2\"\,\ \"metadata\"\:\ null\}\]\}
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/output_taxonomy_1.biom1.re	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,1 @@
+\{\"id\"\:\ \"None\"\,\"format\"\:\ \"Biological\ Observation\ Matrix\ 1\.0\.0\"\,\"format\_url\"\:\ \"http\:\/\/biom\-format\.org\"\,\"matrix\_type\"\:\ \"sparse\"\,\"generated\_by\"\:\ \"BIOM\-Format\ 2\.1\.5\"\,\"date\"\:\ \".*\"\,\"type\"\:\ \"OTU\ table\"\,\"matrix\_element\_type\"\:\ \"float\"\,\"shape\"\:\ \[19\,\ 2\]\,\"data\"\:\ \[\[1\,0\,160\.0\]\,\[1\,1\,242\.0\]\,\[6\,0\,1\.0\]\,\[6\,1\,1\.0\]\,\[7\,0\,3\.0\]\,\[7\,1\,4\.0\]\,\[12\,0\,13\.0\]\,\[12\,1\,36\.0\]\,\[14\,0\,1\.0\]\,\[14\,1\,5\.0\]\,\[15\,0\,1\.0\]\,\[16\,0\,1\.0\]\,\[16\,1\,3\.0\]\]\,\"rows\"\:\ \[\{\"id\"\:\ \"2\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Archaea\"\]\}\}\,\{\"id\"\:\ \"3\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\]\}\}\,\{\"id\"\:\ \"4\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Archaea\"\,\ \"p\_\_Crenarchaeota\"\]\}\}\,\{\"id\"\:\ \"5\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Archaea\"\,\ \"p\_\_Euryarchaeota\"\]\}\}\,\{\"id\"\:\ \"8\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_AC1\"\]\}\}\,\{\"id\"\:\ \"9\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_AD3\"\]\}\}\,\{\"id\"\:\ \"10\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_Acidobacteria\"\]\}\}\,\{\"id\"\:\ \"11\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_Actinobacteria\"\]\}\}\,\{\"id\"\:\ \"12\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_AncK6\"\]\}\}\,\{\"id\"\:\ \"14\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_Armatimonadetes\"\]\}\}\,\{\"id\"\:\ \"15\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_BHI80\-139\"\]\}\}\,\{\"id\"\:\ \"16\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_BRC1\"\]\}\}\,\{\"id\"\:\ \"17\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_Bacteroidetes\"\]\}\}\,\{\"id\"\:\ \"18\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_CD12\"\]\}\}\,\{\"id\"\:\ \"22\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_Chlorobi\"\]\}\}\,\{\"id\"\:\ \"23\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_Chloroflexi\"\]\}\}\,\{\"id\"\:\ \"25\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_Cyanobacteria\"\]\}\}\,\{\"id\"\:\ \"28\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_EM19\"\]\}\}\,\{\"id\"\:\ \"29\"\,\ \"metadata\"\:\ \{\"taxonomy\"\:\ \[\"d\_\_Bacteria\"\,\ \"p\_\_EM3\"\]\}\}\]\,\"columns\"\:\ \[\{\"id\"\:\ \"SAMPLE\_1\"\,\ \"metadata\"\:\ null\}\,\{\"id\"\:\ \"SAMPLE\_2\"\,\ \"metadata\"\:\ null\}\]\}
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/sample_subsetting	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,1 @@
+SAMPLE_1
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/subset_observations.biom	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,23 @@
+{
+"id": "None"
+,
+"format": "Biological Observation Matrix 1.0.0"
+,
+"format_url": "http://biom-format.org"
+,
+"type": "OTU table"
+,
+"generated_by": "BIOM-Format 2.1.5"
+,
+"date": "2016-05-26T17:20:40.263567"
+,
+"matrix_type": "sparse"
+,
+"matrix_element_type": "float"
+,
+"data": [[0,0,3.0],[0,1,4.0],[1,0,1.0]], "shape": [2, 2]
+,
+"rows": [{"id": "11", "metadata": null}, {"id": "23", "metadata": null}]
+,
+"columns": [{"id": "SAMPLE_1", "metadata": null},{"id": "SAMPLE_2", "metadata": null}]
+}
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/subset_samples.biom	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,23 @@
+{
+"id": "None"
+,
+"format": "Biological Observation Matrix 1.0.0"
+,
+"format_url": "http://biom-format.org"
+,
+"type": "OTU table"
+,
+"generated_by": "BIOM-Format 2.1.5"
+,
+"date": "2016-05-26T17:20:40.263567"
+,
+"matrix_type": "sparse"
+,
+"matrix_element_type": "float"
+,
+"data": [[1,0,160.0],[6,0,1.0],[7,0,3.0],[12,0,13.0],[14,0,1.0],[15,0,1.0],[16,0,1.0]], "shape": [19, 1]
+,
+"columns": [{"id": "SAMPLE_1", "metadata": null}]
+,
+"rows": [{"id": "2", "metadata": null},{"id": "3", "metadata": null},{"id": "4", "metadata": null},{"id": "5", "metadata": null},{"id": "8", "metadata": null},{"id": "9", "metadata": null},{"id": "10", "metadata": null},{"id": "11", "metadata": null},{"id": "12", "metadata": null},{"id": "14", "metadata": null},{"id": "15", "metadata": null},{"id": "16", "metadata": null},{"id": "17", "metadata": null},{"id": "18", "metadata": null},{"id": "22", "metadata": null},{"id": "23", "metadata": null},{"id": "25", "metadata": null},{"id": "28", "metadata": null},{"id": "29", "metadata": null}]
+}
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/summarize	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,17 @@
+Num samples: 2
+Num observations: 19
+Total count: 471
+Table density (fraction of non-zero values): 0.342
+
+Counts/sample summary:
+ Min: 180.0
+ Max: 291.0
+ Median: 235.500
+ Mean: 235.500
+ Std. dev.: 55.500
+ Sample Metadata Categories: None provided
+ Observation Metadata Categories: None provided
+
+Counts/sample detail:
+SAMPLE_1: 180.0
+SAMPLE_2: 291.0
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/summarize_qualitative_observations	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,32 @@
+Num samples: 2
+Num observations: 19
+
+Sample/observations summary:
+ Min: 0
+ Max: 2
+ Median: 0.000
+ Mean: 0.684
+ Std. dev.: 0.921
+ Sample Metadata Categories: None provided
+ Observation Metadata Categories: None provided
+
+Observations/sample detail:
+2: 0
+16: 0
+12: 0
+8: 0
+4: 0
+18: 0
+9: 0
+29: 0
+5: 0
+28: 0
+14: 0
+15: 0
+23: 1
+10: 2
+17: 2
+25: 2
+22: 2
+11: 2
+3: 2
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/uc_table.uc	Wed Sep 11 12:41:04 2019 -0400
@@ -0,0 +1,1000 @@
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