Mercurial > repos > iuc > biscot
changeset 0:ef4b5ce71745 draft default tip
planemo upload for repository https://github.com/bgruening/iuc/tree/master/tools/biscot commit cce7b9b092030d3bb23a706ca3bf2dece9c20b5f
author | iuc |
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date | Fri, 06 Jan 2023 22:10:44 +0000 |
parents | |
children | |
files | biscot.xml macros.xml test-data/bionano_key_file.txt test-data/query_cmap_file.cmap test-data/reference_cmap_file.cmap test-data/small.fasta.gz test-data/xml_configuration_file.xmap |
diffstat | 7 files changed, 1344 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/biscot.xml Fri Jan 06 22:10:44 2023 +0000 @@ -0,0 +1,131 @@ +<tool id="biscot" name="BiSCoT" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="20.01"> + <description>Bionano scaffolding correction</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <command detect_errors="exit_code"><![CDATA[ + biscot + --cmap-ref '${cmap_ref}' + --cmap-1 '${cmap_1}' + --xmap-1 '${xmap_1}' + --key $key + --contigs $contigs + #if $secondary_map.cmap_2 + --cmap-2 '${cmap_2}' + --xmap-2 '${xmap_2}' + #end if + #if $xmap_2enz + --xmap-2enz '${xmap_2enz}' + #end if + ## $aggressive # disabled due to dependency conflicts + $only_confirmed_pos + ]]> </command> + <inputs> + <param argument="--cmap-ref" type="data" format="cmap" label="Reference CMAP file" help="This file describes the positions of enzymatic labelling sites on the reference genome" /> + <param argument="--cmap-1" type="data" format="cmap" label="Query CMAP file" help="This file describes the positions of enzymatic labelling sites on the contigs" /> + <param argument="--xmap-1" type="data" format="xml,txt" label="XML configuration file" help="This file describes the alignments of contig labels on the anchor" /> + <param argument="--contigs" type="data" format="fasta" label="Contigs file" help="Contigs file (FASTA) that was scaffolded by the Bionano scaffold" /> + <param argument="--key" type="data" format="tabular" label="Bionano key file" help="Bionano key file giving the correspondance between maps and contigs" /> + <section name="secondary_map" title="Secondary map"> + <param argument="--cmap-2" type="data" format="cmap" optional="true" label="Query CMAP file" help="This file describes the positions of enzymatic labelling sites on the contigs" /> + <param argument="--xmap-2" type="data" format="xml,txt" optional="true" label="XML configuration file" help="This file describes the alignments of contig labels on the anchor" /> + </section> + <param argument="--xmap-2enz" type="data" format="xml,txt" optional="true" label="Two enzymes XML configuration file" help="This argument is used to provide the final XMAP file containing + the mappings of labels of both enzymes. This argument is useful (and recommended) to ensure that no mapping has been missed inside one of the individual XMAP file" /> + <param argument="--aggressive" type="boolean" truevalue="--aggressive" falsevalue="" checked="false" + label="Enable BLAT phase" help=" enables the sequence similarity scaffolding. In a first phase, BiSCoT will search similarities between contigs based on label mappings. If this parameter is set, + BiSCoT will search for sequence similarity to close gaps created by the first step" /> + <param argument="--only-confirmed-pos" type="boolean" truevalue="--only-confirmed-pos" falsevalue="" checked="false" + label="Only confirmed positions" help="To be retained, an alignment position in --xmap-1 or --xmap-2 has to be present in --xmap-2enz" /> + <param name="log_file" type="boolean" truevalue="true" falsevalue="false" checked="false" label="Generate log file" help="Log file can be helpful for debuggin purposes" /> + </inputs> + <outputs> + <data name="log" format="txt" from_work_dir="biscot/biscot.log" label="${tool.name} on ${on_string}: log file"> + <filter>log_file</filter> + </data> + <data name="fasta" format="fasta" from_work_dir="biscot/scaffolds.fasta" label="${tool.name} on ${on_string}: scaffolds (FASTA)"/> + <data name="agp" format="agp" from_work_dir="biscot/scaffolds.agp" label="${tool.name} on ${on_string}: scaffolds (AGP)"/> + </outputs> + <tests> + <test expect_num_outputs="3"> + <param name="cmap_ref" value="reference_cmap_file.cmap"/> + <param name="cmap_1" value="query_cmap_file.cmap"/> + <param name="xmap_1" value="xml_configuration_file.xmap"/> + <param name="contigs" value="small.fasta.gz"/> + <param name="key" value="bionano_key_file.txt"/> + <param name="log_file" value="true"/> + <output name="log"> + <assert_contents> + <has_text text="Final AGP and scaffolds file are in biscot/scaffolds.agp and biscot/scaffolds.fasta"/> + <has_text text="Converting agp file to fasta"/> + <has_n_lines n="13"/> + </assert_contents> + </output> + <output name="fasta"> + <assert_contents> + <has_text text="Super-Scaffold_1"/> + <has_text text="TCATCGGTTAAATCATCGCCCAGAAATACGGGCGT"/> + <has_size value="2097147"/> + </assert_contents> + </output> + <output name="agp"> + <assert_contents> + <has_text text="Super-Scaffold_1"/> + <has_text text="4753350"/> + <has_n_lines n="3"/> + </assert_contents> + </output> + </test> + <test expect_num_outputs="2"> + <param name="cmap_ref" value="reference_cmap_file.cmap"/> + <param name="cmap_1" value="query_cmap_file.cmap"/> + <param name="xmap_1" value="xml_configuration_file.xmap"/> + <param name="contigs" value="small.fasta.gz"/> + <param name="key" value="bionano_key_file.txt"/> + <param name="xmap_2enz" value="xml_configuration_file.xmap"/> + <param name="aggressive" value="true"/> + <param name="only_confirmed_pos" value="true"/> + <section name="secondary_map"> + <param name="cmap_2" value="query_cmap_file.cmap"/> + <param name="xmap_2" value="xml_configuration_file.xmap"/> + </section> + <output name="fasta"> + <assert_contents> + <has_text text="Super-Scaffold_1"/> + <has_text text="TCATCGGTTAAATCATCGCCCAGAAATACGGGCGT"/> + <has_size value="2097147"/> + </assert_contents> + </output> + <output name="agp"> + <assert_contents> + <has_text text="Super-Scaffold_1"/> + <has_text text="4753350"/> + <has_n_lines n="3"/> + </assert_contents> + </output> + </test> + </tests> + <help><![CDATA[ + +.. class:: infomark + +**Purpose** + +BiSCoT is a tool that examinates data generated during a previous Bionano scaffolding and merges contigs separated by a 13-Ns gap if needed. BiSCoT also re-evaluates gap sizes and searches for an +alignment between two contigs if the gap size is inferior to 1,000 nucleotides. BiSCoT is therefore not a traditional scaffolder since it can only be used to improve an existing scaffolding, based +on an optical map. + +----- + +.. class:: infomark + +**Required files** + +During the scaffolding, the Bionano generates a visual representation of the hybrid scaffolds that is called an *anchor*. It also generates one *.key* file, which describes the mapping between map +identifiers and contig names, several CMAP files, which contain the position of enzymatic labelling sites on contig maps and on the anchor, and a XMAP file, that describes the alignment between a contig map +and an anchor. BiSCoT first loads the contigs into memory based on the key file. Then, the anchor CMAP file and contig CMAP files are loaded into memory. Finally, the XMAP file is parsed and loaded. + + ]]> </help> + <expand macro="citations"/> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Fri Jan 06 22:10:44 2023 +0000 @@ -0,0 +1,17 @@ +<macros> + <token name="@TOOL_VERSION@">2.3.3</token> + <token name="@VERSION_SUFFIX@">0</token> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@TOOL_VERSION@">biscot</requirement> + <requirement type="package" version="36">blat</requirement> + <requirement type="package" version="377">ucsc-pslsort</requirement> + <requirement type="package" version="377">ucsc-pslreps</requirement> + </requirements> + </xml> + <xml name="citations"> + <citations> + <citation type="doi">10.7717/peerj.10150</citation> + </citations> + </xml> +</macros>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/bionano_key_file.txt Fri Jan 06 22:10:44 2023 +0000 @@ -0,0 +1,3 @@ +CompntId CompntName CompntLength +1 Super_scaffold_3 4753350 +4 Super_scaffold_3 4753350
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/query_cmap_file.cmap Fri Jan 06 22:10:44 2023 +0000 @@ -0,0 +1,545 @@ +# hostname=6d61d934e61b +# $ cd /HybridScaffold; /RefAligner/amd2/RefAligner -o /data/jwd03f/main/053/913/53913897/working/align_final/bionano_bppAdjust_cmap_ngs_fasta_BNGcontigs_HYBRID_SCAFFOLD -stdout -stderr -i /data/jwd03f/main/053/913/53913897/working/assignAlignType/assignAlignType_q.cmap -ref /data/jwd03f/main/053/913/53913897/working/align_final/step2.hybrid.cmap -maxmem 250 -maxthreads 24 -maxvirtmem 250 -RAmem 3 1 -res 2.9 -FP 0.6 -FN 0.06 -sf 0.20 -sd 0.0 -sr 0.01 -extend 1 -outlier 0.0001 -endoutlier 0.001 -PVendoutlier -deltaX 6 -deltaY 6 -xmapchim 12 -hashgen 5 7 2.4 1.5 0.05 5.0 1 1 4 -hash -hashdelta 26 10 46 -hashMultiMatch 30 10 -insertThreads 24 -nosplit 2 -biaswt 0 -T 1e-10 -S -1000 -indel -PVres 2 -rres 0.9 -MaxSE 0.5 -HSDrange 1.0 -outlierBC -xmapUnique 14 -AlignRes 2. -outlierExtend 6 24 -Kmax 6 -resEstimate -BestRef 1 -f -mres 0.9 +# CompileDir= /home/users/sqa01/RefAlignerBuild/branches/12432.12463rel/12432.12463rel CompileCmd=/opt/gcc-9.3.0/bin/g++ -B/opt/binutils-2.34 -fopenmp -Ofast -march=znver2 -mavx2 -mfpmath=sse -DUSE_PFLOAT=1 -DUSE_RFLOAT=1 -DUSE_SSE=1 -DUSE_MFLOAT=1 -DUSE_EPOW=2 -I/home/users8/tanantharaman/sleef-081120/build/include -I/opt/glibc-2.31/include -DUSE_STATIC -freciprocal-math -fno-signed-zeros -fno-trapping-math -fno-tree-vectorize -mtune=znver2 -fno-lto-odr-type-merging -DRELEASE=1 -std=gnu++98 -Wl,--dynamic-linker=/opt/glibc-2.31/lib/ld-linux-x86-64.so.2 -Wl,--rpath=/opt/glibc-2.31/lib -Wl,--rpath=/opt/gcc-9.3.0/lib64 -lsleefinline -lrt -lmvec -L/home/users8/tanantharaman/sleef-081120/build/lib -L/opt/glibc-2.31/lib -L/opt/gcc-9.3.0/lib64 -static -static-libstdc++ -static-libgcc -s SVNversion=12463 $Header: http://svn.bnm.local:81/svn/Informatics/RefAligner/branches/12432/RefAligner.cpp 12458 2021-10-24 00:56:45Z tanantharaman $ +# FLAGS: USE_SSE=1 USE_AVX=1 USE_MIC=0 USE_PFLOAT=1 USE_RFLOAT=1 USE_MFLOAT=1 USE_EPOW=2 DEBUG=1 VERB=1 +# CMAP File Version: 0.1 +# Label Channels: 1 +# Nickase Recognition Site 1: gctcttc +# Number of Consensus Maps: 2 +# StdDev & Coverage refer to the interval between current site and next site +#h CMapId ContigLength NumSites SiteID LabelChannel Position StdDev Coverage Occurrence +#f int float int int int float float float float +1 3879935.1 449 1 1 20.0 112.1 106.0 106.0 +1 3879935.1 449 2 1 1679.6 290.0 108.0 106.0 +1 3879935.1 449 3 1 45612.1 126.1 114.0 92.0 +1 3879935.1 449 4 1 49245.1 205.8 116.0 100.0 +1 3879935.1 449 5 1 68514.5 123.8 120.0 116.0 +1 3879935.1 449 6 1 71807.2 139.9 126.0 118.0 +1 3879935.1 449 7 1 77614.8 168.6 126.0 116.0 +1 3879935.1 449 8 1 88645.4 147.4 126.0 104.0 +1 3879935.1 449 9 1 95715.0 140.5 130.0 110.0 +1 3879935.1 449 10 1 101612.6 182.6 132.0 118.0 +1 3879935.1 449 11 1 115551.9 136.8 134.0 108.0 +1 3879935.1 449 12 1 120842.6 155.7 136.0 128.0 +1 3879935.1 449 13 1 129405.4 134.2 138.0 136.0 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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reference_cmap_file.cmap Fri Jan 06 22:10:44 2023 +0000 @@ -0,0 +1,636 @@ +# hostname=6d61d934e61b +# $ cd /HybridScaffold; /RefAligner/amd2/RefAligner -o /data/jwd03f/main/053/913/53913897/working/align_final/bionano_bppAdjust_cmap_ngs_fasta_BNGcontigs_HYBRID_SCAFFOLD -stdout -stderr -i /data/jwd03f/main/053/913/53913897/working/assignAlignType/assignAlignType_q.cmap -ref /data/jwd03f/main/053/913/53913897/working/align_final/step2.hybrid.cmap -maxmem 250 -maxthreads 24 -maxvirtmem 250 -RAmem 3 1 -res 2.9 -FP 0.6 -FN 0.06 -sf 0.20 -sd 0.0 -sr 0.01 -extend 1 -outlier 0.0001 -endoutlier 0.001 -PVendoutlier -deltaX 6 -deltaY 6 -xmapchim 12 -hashgen 5 7 2.4 1.5 0.05 5.0 1 1 4 -hash -hashdelta 26 10 46 -hashMultiMatch 30 10 -insertThreads 24 -nosplit 2 -biaswt 0 -T 1e-10 -S -1000 -indel -PVres 2 -rres 0.9 -MaxSE 0.5 -HSDrange 1.0 -outlierBC -xmapUnique 14 -AlignRes 2. -outlierExtend 6 24 -Kmax 6 -resEstimate -BestRef 1 -f -mres 0.9 +# CompileDir= /home/users/sqa01/RefAlignerBuild/branches/12432.12463rel/12432.12463rel CompileCmd=/opt/gcc-9.3.0/bin/g++ -B/opt/binutils-2.34 -fopenmp -Ofast -march=znver2 -mavx2 -mfpmath=sse -DUSE_PFLOAT=1 -DUSE_RFLOAT=1 -DUSE_SSE=1 -DUSE_MFLOAT=1 -DUSE_EPOW=2 -I/home/users8/tanantharaman/sleef-081120/build/include -I/opt/glibc-2.31/include -DUSE_STATIC -freciprocal-math -fno-signed-zeros -fno-trapping-math -fno-tree-vectorize -mtune=znver2 -fno-lto-odr-type-merging -DRELEASE=1 -std=gnu++98 -Wl,--dynamic-linker=/opt/glibc-2.31/lib/ld-linux-x86-64.so.2 -Wl,--rpath=/opt/glibc-2.31/lib -Wl,--rpath=/opt/gcc-9.3.0/lib64 -lsleefinline -lrt -lmvec -L/home/users8/tanantharaman/sleef-081120/build/lib -L/opt/glibc-2.31/lib -L/opt/gcc-9.3.0/lib64 -static -static-libstdc++ -static-libgcc -s SVNversion=12463 $Header: http://svn.bnm.local:81/svn/Informatics/RefAligner/branches/12432/RefAligner.cpp 12458 2021-10-24 00:56:45Z tanantharaman $ +# FLAGS: USE_SSE=1 USE_AVX=1 USE_MIC=0 USE_PFLOAT=1 USE_RFLOAT=1 USE_MFLOAT=1 USE_EPOW=2 DEBUG=1 VERB=1 +# CMAP File Version: 0.1 +# Label Channels: 1 +# Nickase Recognition Site 1: gctcttc +# Number of Consensus Maps: 1 +# StdDev refers to the interval between the current site and the next site +#h CMapId ContigLength NumSites SiteID LabelChannel Position StdDev Coverage Occurrence Mask +#f int float int int int float float float float Hex +1 4753350.0 624 1 1 4239.0 0.0 0.0 0.0 10 +1 4753350.0 624 2 1 17279.0 0.0 0.0 0.0 0 +1 4753350.0 624 3 1 19489.5 0.0 1.0 1.0 0 +1 4753350.0 624 4 1 37090.0 0.0 1.0 1.0 0 +1 4753350.0 624 5 1 55531.0 0.0 1.0 1.0 0 +1 4753350.0 624 6 1 58111.0 0.0 1.0 1.0 0 +1 4753350.0 624 7 1 93858.0 0.0 1.0 1.0 0 +1 4753350.0 624 8 1 97892.0 0.0 1.0 1.0 0 +1 4753350.0 624 9 1 105928.0 0.0 1.0 1.0 0 +1 4753350.0 624 10 1 108648.0 0.0 1.0 1.0 0 +1 4753350.0 624 11 1 109729.0 0.0 1.0 1.0 0 +1 4753350.0 624 12 1 116817.0 0.0 1.0 1.0 0 +1 4753350.0 624 13 1 128506.0 0.0 1.0 1.0 0 +1 4753350.0 624 14 1 144099.0 0.0 1.0 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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/xml_configuration_file.xmap Fri Jan 06 22:10:44 2023 +0000 @@ -0,0 +1,12 @@ +# hostname=6d61d934e61b +# $ cd /HybridScaffold; /RefAligner/amd2/RefAligner -o /data/jwd03f/main/053/913/53913897/working/align_final/bionano_bppAdjust_cmap_ngs_fasta_BNGcontigs_HYBRID_SCAFFOLD -stdout -stderr -i /data/jwd03f/main/053/913/53913897/working/assignAlignType/assignAlignType_q.cmap -ref /data/jwd03f/main/053/913/53913897/working/align_final/step2.hybrid.cmap -maxmem 250 -maxthreads 24 -maxvirtmem 250 -RAmem 3 1 -res 2.9 -FP 0.6 -FN 0.06 -sf 0.20 -sd 0.0 -sr 0.01 -extend 1 -outlier 0.0001 -endoutlier 0.001 -PVendoutlier -deltaX 6 -deltaY 6 -xmapchim 12 -hashgen 5 7 2.4 1.5 0.05 5.0 1 1 4 -hash -hashdelta 26 10 46 -hashMultiMatch 30 10 -insertThreads 24 -nosplit 2 -biaswt 0 -T 1e-10 -S -1000 -indel -PVres 2 -rres 0.9 -MaxSE 0.5 -HSDrange 1.0 -outlierBC -xmapUnique 14 -AlignRes 2. -outlierExtend 6 24 -Kmax 6 -resEstimate -BestRef 1 -f -mres 0.9 +# CompileDir= /home/users/sqa01/RefAlignerBuild/branches/12432.12463rel/12432.12463rel CompileCmd=/opt/gcc-9.3.0/bin/g++ -B/opt/binutils-2.34 -fopenmp -Ofast -march=znver2 -mavx2 -mfpmath=sse -DUSE_PFLOAT=1 -DUSE_RFLOAT=1 -DUSE_SSE=1 -DUSE_MFLOAT=1 -DUSE_EPOW=2 -I/home/users8/tanantharaman/sleef-081120/build/include -I/opt/glibc-2.31/include -DUSE_STATIC -freciprocal-math -fno-signed-zeros -fno-trapping-math -fno-tree-vectorize -mtune=znver2 -fno-lto-odr-type-merging -DRELEASE=1 -std=gnu++98 -Wl,--dynamic-linker=/opt/glibc-2.31/lib/ld-linux-x86-64.so.2 -Wl,--rpath=/opt/glibc-2.31/lib -Wl,--rpath=/opt/gcc-9.3.0/lib64 -lsleefinline -lrt -lmvec -L/home/users8/tanantharaman/sleef-081120/build/lib -L/opt/glibc-2.31/lib -L/opt/gcc-9.3.0/lib64 -static -static-libstdc++ -static-libgcc -s SVNversion=12463 $Header: http://svn.bnm.local:81/svn/Informatics/RefAligner/branches/12432/RefAligner.cpp 12458 2021-10-24 00:56:45Z tanantharaman $ +# FLAGS: USE_SSE=1 USE_AVX=1 USE_MIC=0 USE_PFLOAT=1 USE_RFLOAT=1 USE_MFLOAT=1 USE_EPOW=2 DEBUG=1 VERB=1 +# XMAP File Version: 0.2 +# Label Channels: 1 +# Reference Maps From: /data/jwd03f/main/053/913/53913897/working/align_final/bionano_bppAdjust_cmap_ngs_fasta_BNGcontigs_HYBRID_SCAFFOLD_r.cmap +# Query Maps From: /data/jwd03f/main/053/913/53913897/working/align_final/bionano_bppAdjust_cmap_ngs_fasta_BNGcontigs_HYBRID_SCAFFOLD_q.cmap +#h XmapEntryID QryContigID RefContigID QryStartPos QryEndPos RefStartPos RefEndPos Orientation Confidence HitEnum QryLen RefLen LabelChannel Alignment +#f int int int float float float float string float string float float int string +1 4 1 20.0 715071.0 19489.5 743436.5 + 92.15 7M1D12M1D8M1D2M1D9M1D4M1D10M1D1M1D23M1D2M1D5M 715091.0 4753350.0 1 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