# HG changeset patch # User iuc # Date 1673530138 0 # Node ID bb935709506a3e154cbf7ad4c84adf526b1268b8 # Parent 41030a6c03b7f0c7d0d249a6867a9e50fb8ae97b planemo upload commit f7f94fe1eccafe1e29657bbd79197b4b32e03ffe diff -r 41030a6c03b7 -r bb935709506a busco.xml --- a/busco.xml Mon Apr 11 09:02:11 2022 +0000 +++ b/busco.xml Thu Jan 12 13:28:58 2023 +0000 @@ -1,11 +1,11 @@ assess genome assembly and annotation completeness + + macros.xml + busco - - macros.xml - busco tar @@ -13,16 +13,21 @@ busco --version busco_output.gff + && + ## gff files can be absent + cat busco_galaxy/run_*/busco_sequences/*busco_sequences/*.gff >> busco_output.gff 2> /dev/null || true +#end if + ]]> @@ -123,6 +136,7 @@ +
@@ -141,9 +155,12 @@ outputs and 'image' in outputs + + outputs and 'gff' in outputs + - + @@ -155,16 +172,22 @@ - + + + + + + + - + @@ -173,13 +196,19 @@ - + + + + + + + - + @@ -188,13 +217,14 @@ - + + - + @@ -210,9 +240,15 @@ - + + + + + + + @@ -235,7 +271,7 @@ - + @@ -247,13 +283,14 @@ - + + - + @@ -265,11 +302,12 @@ - + + - 5.3.2 + 5.4.4 0 diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results/full_table --- a/test-data/genome_results/full_table Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/genome_results/full_table Thu Jan 12 13:28:58 2023 +0000 @@ -1,4 +1,4 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) # Busco id Status Sequence Gene Start Gene End Strand Score Length OrthoDB url Description 774at6656 Missing diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results/missing_buscos_list --- a/test-data/genome_results/missing_buscos_list Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/genome_results/missing_buscos_list Thu Jan 12 13:28:58 2023 +0000 @@ -1,4 +1,4 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) # Busco id 100070at6656 diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results/short_summary --- a/test-data/genome_results/short_summary Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/genome_results/short_summary Thu Jan 12 13:28:58 2023 +0000 @@ -1,7 +1,7 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) -# Summarized benchmarking in BUSCO notation for file /tmp/tmpctznxi43/files/9/e/c/dataset_9ec37448-e146-4536-b66f-9082e1734ea4.dat -# BUSCO was run in mode: genome +# Summarized benchmarking in BUSCO notation for file /tmp/tmprqvmp912/files/000/dataset_22.dat +# BUSCO was run in mode: euk_genome_aug # Gene predictor used: augustus ***** Results: ***** @@ -14,11 +14,22 @@ 1012 Missing BUSCOs (M) 1013 Total BUSCO groups searched +Assembly Statistics: + 1 Number of scaffolds + 1 Number of contigs + 62370 Total length + 0.000% Percent gaps + 62 KB Scaffold N50 + 62 KB Contigs N50 + + Dependencies and versions: hmmsearch: 3.1 - makeblastdb: 2.12.0+ - tblastn: 2.12.0+ - augustus: 3.4.0 + bbtools: 39.01 + makeblastdb: 2.13.0+ + tblastn: 2.13.0+ + augustus: 3.5.0 gff2gbSmallDNA.pl: None new_species.pl: None etraining: None + busco: 5.4.4 diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results_metaeuk/full_table --- a/test-data/genome_results_metaeuk/full_table Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/genome_results_metaeuk/full_table Thu Jan 12 13:28:58 2023 +0000 @@ -1,4 +1,4 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) # Busco id Status Sequence Gene Start Gene End Strand Score Length OrthoDB url Description 774at6656 Missing diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results_metaeuk/missing_buscos_list --- a/test-data/genome_results_metaeuk/missing_buscos_list Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/genome_results_metaeuk/missing_buscos_list Thu Jan 12 13:28:58 2023 +0000 @@ -1,4 +1,4 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) # Busco id 100070at6656 diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results_metaeuk/out.gff --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/genome_results_metaeuk/out.gff Thu Jan 12 13:28:58 2023 +0000 @@ -0,0 +1,13 @@ +##gff-version 3 +sample MetaEuk gene 40256 42071 198 + . Target_ID=68987at6656;TCS_ID=68987at6656|sample|+|40255 +sample MetaEuk mRNA 40256 42071 198 + . Target_ID=68987at6656;TCS_ID=68987at6656|sample|+|40255_mRNA;Parent=68987at6656|sample|+|40255 +sample MetaEuk exon 40256 40390 49 + . Target_ID=68987at6656;TCS_ID=68987at6656|sample|+|40255_exon_0;Parent=68987at6656|sample|+|40255_mRNA +sample MetaEuk CDS 40256 40390 49 + . Target_ID=68987at6656;TCS_ID=68987at6656|sample|+|40255_CDS_0;Parent=68987at6656|sample|+|40255_exon_0 +sample MetaEuk exon 40653 41168 94 + . Target_ID=68987at6656;TCS_ID=68987at6656|sample|+|40255_exon_1;Parent=68987at6656|sample|+|40255_mRNA +sample MetaEuk CDS 40653 41168 94 + . Target_ID=68987at6656;TCS_ID=68987at6656|sample|+|40255_CDS_1;Parent=68987at6656|sample|+|40255_exon_1 +sample MetaEuk exon 41718 42071 65 + . Target_ID=68987at6656;TCS_ID=68987at6656|sample|+|40255_exon_2;Parent=68987at6656|sample|+|40255_mRNA +sample MetaEuk CDS 41718 42071 65 + . Target_ID=68987at6656;TCS_ID=68987at6656|sample|+|40255_CDS_2;Parent=68987at6656|sample|+|40255_exon_2 +sample MetaEuk gene 34846 35679 545 - . Target_ID=94238at6656_7227_0:001f92;TCS_ID=94238at6656_7227_0:001f92|sample|-|34845 +sample MetaEuk mRNA 34846 35679 545 - . Target_ID=94238at6656_7227_0:001f92;TCS_ID=94238at6656_7227_0:001f92|sample|-|34845_mRNA;Parent=94238at6656_7227_0:001f92|sample|-|34845 +sample MetaEuk exon 34846 35679 545 - . Target_ID=94238at6656_7227_0:001f92;TCS_ID=94238at6656_7227_0:001f92|sample|-|34845_exon_0;Parent=94238at6656_7227_0:001f92|sample|-|34845_mRNA +sample MetaEuk CDS 34846 35679 545 - . Target_ID=94238at6656_7227_0:001f92;TCS_ID=94238at6656_7227_0:001f92|sample|-|34845_CDS_0;Parent=94238at6656_7227_0:001f92|sample|-|34845_exon_0 diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results_metaeuk/short_summary --- a/test-data/genome_results_metaeuk/short_summary Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/genome_results_metaeuk/short_summary Thu Jan 12 13:28:58 2023 +0000 @@ -1,7 +1,7 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) -# Summarized benchmarking in BUSCO notation for file /tmp/tmpctznxi43/files/9/6/4/dataset_96441510-fb85-41dd-a4ce-8a20d74173a9.dat -# BUSCO was run in mode: genome +# Summarized benchmarking in BUSCO notation for file /tmp/tmprqvmp912/files/000/dataset_28.dat +# BUSCO was run in mode: euk_genome_met # Gene predictor used: metaeuk ***** Results: ***** @@ -14,6 +14,17 @@ 1011 Missing BUSCOs (M) 1013 Total BUSCO groups searched +Assembly Statistics: + 1 Number of scaffolds + 1 Number of contigs + 62370 Total length + 0.000% Percent gaps + 62 KB Scaffold N50 + 62 KB Contigs N50 + + Dependencies and versions: hmmsearch: 3.1 - metaeuk: 4.a0f584d + bbtools: 39.01 + metaeuk: 6.a5d39d9 + busco: 5.4.4 diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results_metaeuk/summary.png Binary file test-data/genome_results_metaeuk/summary.png has changed diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results_metaeuk_auto/full_table --- a/test-data/genome_results_metaeuk_auto/full_table Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/genome_results_metaeuk_auto/full_table Thu Jan 12 13:28:58 2023 +0000 @@ -1,4 +1,4 @@ -# BUSCO version is: 5.3.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: eukaryota_odb10 (Creation date: 2020-09-10, number of genomes: 70, number of BUSCOs: 255) # Busco id Status Sequence Gene Start Gene End Strand Score Length 39650at2759 Missing diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results_metaeuk_auto/missing_buscos_list --- a/test-data/genome_results_metaeuk_auto/missing_buscos_list Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/genome_results_metaeuk_auto/missing_buscos_list Thu Jan 12 13:28:58 2023 +0000 @@ -1,4 +1,4 @@ -# BUSCO version is: 5.3.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: eukaryota_odb10 (Creation date: 2020-09-10, number of genomes: 70, number of BUSCOs: 255) # Busco id 1001705at2759 diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results_metaeuk_auto/out.gff --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/genome_results_metaeuk_auto/out.gff Thu Jan 12 13:28:58 2023 +0000 @@ -0,0 +1,5 @@ +##gff-version 3 +sample MetaEuk gene 34846 35679 545 - . Target_ID=1053181at2759_7227_0:001f92;TCS_ID=1053181at2759_7227_0:001f92|sample|-|34845 +sample MetaEuk mRNA 34846 35679 545 - . Target_ID=1053181at2759_7227_0:001f92;TCS_ID=1053181at2759_7227_0:001f92|sample|-|34845_mRNA;Parent=1053181at2759_7227_0:001f92|sample|-|34845 +sample MetaEuk exon 34846 35679 545 - . Target_ID=1053181at2759_7227_0:001f92;TCS_ID=1053181at2759_7227_0:001f92|sample|-|34845_exon_0;Parent=1053181at2759_7227_0:001f92|sample|-|34845_mRNA +sample MetaEuk CDS 34846 35679 545 - . Target_ID=1053181at2759_7227_0:001f92;TCS_ID=1053181at2759_7227_0:001f92|sample|-|34845_CDS_0;Parent=1053181at2759_7227_0:001f92|sample|-|34845_exon_0 diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results_metaeuk_auto/short_summary --- a/test-data/genome_results_metaeuk_auto/short_summary Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/genome_results_metaeuk_auto/short_summary Thu Jan 12 13:28:58 2023 +0000 @@ -1,7 +1,7 @@ -# BUSCO version is: 5.3.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: eukaryota_odb10 (Creation date: 2020-09-10, number of genomes: 70, number of BUSCOs: 255) -# Summarized benchmarking in BUSCO notation for file /tmp/tmp5e3tdhzs/files/c/9/b/dataset_c9b61090-5a2d-4dd0-8fd1-d147a4e1573c.dat -# BUSCO was run in mode: genome +# Summarized benchmarking in BUSCO notation for file /tmp/tmprqvmp912/files/000/dataset_34.dat +# BUSCO was run in mode: euk_genome_met # Gene predictor used: metaeuk ***** Results: ***** @@ -14,7 +14,18 @@ 254 Missing BUSCOs (M) 255 Total BUSCO groups searched +Assembly Statistics: + 1 Number of scaffolds + 1 Number of contigs + 62370 Total length + 0.000% Percent gaps + 62 KB Scaffold N50 + 62 KB Contigs N50 + + Dependencies and versions: hmmsearch: 3.1 + bbtools: 39.01 prodigal: 2.6.3 - metaeuk: 5.34c21f2 + busco: 5.4.4 + metaeuk: 6.a5d39d9 diff -r 41030a6c03b7 -r bb935709506a test-data/genome_results_metaeuk_auto/summary.png Binary file test-data/genome_results_metaeuk_auto/summary.png has changed diff -r 41030a6c03b7 -r bb935709506a test-data/proteome_results/full_table --- a/test-data/proteome_results/full_table Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/proteome_results/full_table Thu Jan 12 13:28:58 2023 +0000 @@ -1,4 +1,4 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) # Busco id Status Sequence Score Length OrthoDB url Description 774at6656 Missing diff -r 41030a6c03b7 -r bb935709506a test-data/proteome_results/missing_buscos_list --- a/test-data/proteome_results/missing_buscos_list Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/proteome_results/missing_buscos_list Thu Jan 12 13:28:58 2023 +0000 @@ -1,4 +1,4 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) # Busco id 100070at6656 diff -r 41030a6c03b7 -r bb935709506a test-data/proteome_results/short_summary --- a/test-data/proteome_results/short_summary Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/proteome_results/short_summary Thu Jan 12 13:28:58 2023 +0000 @@ -1,6 +1,6 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) -# Summarized benchmarking in BUSCO notation for file /tmp/tmpctznxi43/files/8/5/3/dataset_853c93b4-f61b-4a14-a96c-ade0e2f5bbbd.dat +# Summarized benchmarking in BUSCO notation for file /tmp/tmprqvmp912/files/000/dataset_6.dat # BUSCO was run in mode: proteins ***** Results: ***** @@ -15,3 +15,4 @@ Dependencies and versions: hmmsearch: 3.1 + busco: 5.4.4 diff -r 41030a6c03b7 -r bb935709506a test-data/proteome_results/summary.png Binary file test-data/proteome_results/summary.png has changed diff -r 41030a6c03b7 -r bb935709506a test-data/transcriptome_results/full_table --- a/test-data/transcriptome_results/full_table Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/transcriptome_results/full_table Thu Jan 12 13:28:58 2023 +0000 @@ -1,4 +1,4 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) # Busco id Status Sequence Score Length OrthoDB url Description 774at6656 Missing diff -r 41030a6c03b7 -r bb935709506a test-data/transcriptome_results/missing_buscos_list --- a/test-data/transcriptome_results/missing_buscos_list Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/transcriptome_results/missing_buscos_list Thu Jan 12 13:28:58 2023 +0000 @@ -1,4 +1,4 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) # Busco id 100070at6656 diff -r 41030a6c03b7 -r bb935709506a test-data/transcriptome_results/out.gff --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/transcriptome_results/out.gff Thu Jan 12 13:28:58 2023 +0000 @@ -0,0 +1,5 @@ +##gff-version 3 +BUSCOaEOG7B0HST MetaEuk gene 1 2700 1493 + . Target_ID=22364at6656_211228_0:002349;TCS_ID=22364at6656_211228_0:002349|BUSCOaEOG7B0HST|+|0 +BUSCOaEOG7B0HST MetaEuk mRNA 1 2700 1493 + . Target_ID=22364at6656_211228_0:002349;TCS_ID=22364at6656_211228_0:002349|BUSCOaEOG7B0HST|+|0_mRNA;Parent=22364at6656_211228_0:002349|BUSCOaEOG7B0HST|+|0 +BUSCOaEOG7B0HST MetaEuk exon 1 2700 1493 + . Target_ID=22364at6656_211228_0:002349;TCS_ID=22364at6656_211228_0:002349|BUSCOaEOG7B0HST|+|0_exon_0;Parent=22364at6656_211228_0:002349|BUSCOaEOG7B0HST|+|0_mRNA +BUSCOaEOG7B0HST MetaEuk CDS 1 2700 1493 + . Target_ID=22364at6656_211228_0:002349;TCS_ID=22364at6656_211228_0:002349|BUSCOaEOG7B0HST|+|0_CDS_0;Parent=22364at6656_211228_0:002349|BUSCOaEOG7B0HST|+|0_exon_0 diff -r 41030a6c03b7 -r bb935709506a test-data/transcriptome_results/short_summary --- a/test-data/transcriptome_results/short_summary Mon Apr 11 09:02:11 2022 +0000 +++ b/test-data/transcriptome_results/short_summary Thu Jan 12 13:28:58 2023 +0000 @@ -1,7 +1,7 @@ -# BUSCO version is: 5.2.1 +# BUSCO version is: 5.4.4 # The lineage dataset is: arthropoda_odb10 (Creation date: 2020-09-10, number of genomes: 90, number of BUSCOs: 1013) -# Summarized benchmarking in BUSCO notation for file /tmp/tmpctznxi43/files/b/5/f/dataset_b5fc3813-7cbb-4aa0-b30c-dc2a6e85905d.dat -# BUSCO was run in mode: transcriptome +# Summarized benchmarking in BUSCO notation for file /tmp/tmprqvmp912/files/000/dataset_12.dat +# BUSCO was run in mode: euk_tran ***** Results: ***** @@ -15,4 +15,5 @@ Dependencies and versions: hmmsearch: 3.1 - metaeuk: 4.a0f584d + metaeuk: 6.a5d39d9 + busco: 5.4.4 diff -r 41030a6c03b7 -r bb935709506a test-data/transcriptome_results/summary.png Binary file test-data/transcriptome_results/summary.png has changed