# HG changeset patch # User iuc # Date 1717346303 0 # Node ID cf1322aeb137f06d8157dec0e3ba56da9892c1e8 # Parent d82648ad25a31206e255d2b8a8fdfe9023c046ab planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwameth commit 16f1bd9f85b90ad12dbb43bc00ebe469620cfd04 diff -r d82648ad25a3 -r cf1322aeb137 bwameth.xml --- a/bwameth.xml Mon Mar 20 20:39:36 2023 +0000 +++ b/bwameth.xml Sun Jun 02 16:38:23 2024 +0000 @@ -1,7 +1,7 @@ Fast and accurate aligner of BS-Seq reads. - 0.2.6 + 0.2.7 bwameth @@ -78,7 +78,8 @@ #else: $read1 $read2 #end if -| samtools view --no-PG -u - | samtools sort --no-PG -@ "\${GALAXY_SLOTS:-4}" -T "\${TMPDIR:-.}" -O bam -o output.bam - +| samtools sort -l 0 -T "\${TMPDIR:-.}" -O bam | samtools view -O bam -@ \${GALAXY_SLOTS:-1} -o output.bam + ]]> @@ -153,7 +154,7 @@ **What it does** -BWA-meth performs alignment of reads in a bisulfite-sequencing experiment (e.g., RRBS or WGBS) to a genome. The methodology employed for this is similar to bismark, where both the reads and the reference genome are *in silico* converted prior to alignment. Methylation extraction on the resulting BAM file can be done with the PileOMeth tool. +BWA-meth performs the alignment of reads in a bisulfite-sequencing experiment (e.g., RRBS or WGBS) to a genome. This methodology is similar to bismark, where both the reads and the reference genome are *in silico* converted prior to alignment. Methylation extraction on the resulting BAM file can be done with the PileOMeth tool. ]]> @misc{1401.1129,