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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/cnvkit commit bced16a45d19b243a244a093c3b3504b2640d5e0
author iuc
date Mon, 22 Apr 2024 17:44:27 +0000
parents c4a22d0fdba5
children
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<tool id="cnvkit_segment" name="CNVkit Segment" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="21.05">
    <description>Infer copy number segments from the given coverage table</description>
    <macros>
        <import>macros.xml</import>
    </macros>
    <expand macro="xrefs"/>
    <expand macro="creators"/>
    <expand macro="requirements"/>
    <command detect_errors="exit_code"><![CDATA[  
        ln -s '$filename' ./tumor.cnr &&
        #if $additional_SNP_allelic_process.vcf
            ln -s '$additional_SNP_allelic_process.vcf' ./vcf_file.vcf &&
        #end if
        #import os
        cnvkit.py segment
            ./tumor.cnr
            --output sample.cns
            --processes \${GALAXY_SLOTS:-4}
            #if $advanced_settings.dataframe
                --dataframe '$advanced_settings.dataframe'
            #end if
            #if $advanced_settings.method == "cbs"
                #set $method_val = "cbs"
                --method '$method_val'
            #else
                --method '$advanced_settings.method'
            #end if
            #if str($advanced_settings.threshold)
                --threshold $advanced_settings.threshold
            #end if
            $advanced_settings.drop_low_coverage
            #if str($advanced_settings.drop_outliers)
                 --drop-outliers $advanced_settings.drop_outliers
            #end if
            $advanced_settings.smooth_cbs
            #if $additional_SNP_allelic_process.vcf
                 --vcf ./vcf_file.vcf
            #end if
            #if $additional_SNP_allelic_process.sample_id
                --sample-id '$additional_SNP_allelic_process.sample_id'
            #end if
            #if $additional_SNP_allelic_process.normal_id
                 --normal-id '$additional_SNP_allelic_process.normal_id'
            #end if
            #if str($additional_SNP_allelic_process.min_variant_depth)
                 --min-variant-depth $additional_SNP_allelic_process.min_variant_depth
            #end if
            #if str($additional_SNP_allelic_process.zygosity_freq)
                 --zygosity-freq $additional_SNP_allelic_process.zygosity_freq
            #end if
    ]]></command>
    <inputs>
        <param name="filename" type="data" format="tabular" label="Bin-Level log2 Ratios/Coverages cnr file" help="Use the output of the CNVkit fix" />
        <section name="additional_SNP_allelic_process" title="additional process for SNP b_allele frequencies" expanded="false">
            <expand macro="additionally_SNP_process" />
        </section>
        <section name="advanced_settings" title="Advanced settings" expanded="false">
            <expand macro="segment_optional" />
        </section>
    </inputs>
    <outputs>
        <data name="out_sample_segment" format="tabular" label="${tool.name} on ${on_string}: Sample segment" from_work_dir="sample.cns" />
    </outputs>
    <tests>
        <test expect_num_outputs="1">
            <param name="filename" ftype="tabular" value="tumor.cnr" />
            <param name="method" value="hmm" />
            <param name="threshold" value="2" />
            <param name="drop_outliers" value="2" />
            <param name="drop_low_coverage" value="1" />
            <output name="out_sample_segment">
                <assert_contents><has_text text="chromosome"/></assert_contents>
            </output>
        </test>
    </tests>
    <help><![CDATA[
         Infer copy number segments from the given coverage table. Segmentation runs independently on
         each chromosome arm, and can be parallelized with the processes option (except for the HMM methods), similar to batch
         
         Segmented log2 ratios (.cns) output file contains those columns
          chromosome, Start, end, gene, log2, depth, weight and number of bins covered by the segment (probes)
    ]]></help>
    <expand macro="citations" />
</tool>