view mapsembler2.xml @ 4:e7c5b81b3c22 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/colibread commit 0ed02865f048ad7471ea79509c0e23162bc29166"

<?xml version='1.0' encoding='utf-8'?>
<tool profile="16.04" id="mapsembler2" name="Mapsembler2" version="2.2.4">
    <description>is a targeted assembly software</description>
    <macros>
        <import>macros.xml</import>
    </macros>
    <requirements>
        <requirement type="package" version="2.2.4">mapsembler2</requirement>
    </requirements>
    <command><![CDATA[

        #set $starter_filename = os.path.basename(str($s)) + "." + $s.ext
        ln -sf '${s}' '${starter_filename}' &&

        #set $samples = []
        #for $read in $r
            #set $filename = os.path.basename(str($read)) + "." + $read.ext
            ln -sf '${read}' '${filename}' &&
            #silent $samples.append($filename)
        #end for

        run_mapsembler2_pipeline.sh
        -s '${starter_filename}'
        -r "${ ' '.join(['%s' % read for read in $samples]) }"
        -t ${t}
        -k ${k}
        -c ${c}
        -d ${d}
        -g ${g}
        -f ${f}
        -x ${x}
        -y ${y}
    ]]></command>

    <inputs>
        <!-- Input data files -->
        <param argument="-s" type="data" format="fasta" label="Starters" help="Set of input sequences" />
        <param argument="-r" type="data" multiple="true" format="fasta,fastq" label="List of reads" />
        <param argument="-t" type="select" label="Select your output extension type">
            <option value="1">a strict sequence</option>
            <option value="2">a consensus sequence</option>
        </param>
        <param argument="-k" type="integer" label="Size of kmers" value="31" help="Set the length of used kmers. Must fit the compiled value. Only uneven number" />
        <param argument="-c" type="integer" label="Minimal coverage" value="5" help="Set the minimal coverage: Used by Phaser (don't use kmers with lower coverage) "/>
        <param argument="-d" type="integer" label="Number of authorized substitutions" value="1" help="Set the number of authorized substitutions used while mapping reads on finding SNPs"/>
        <param argument="-g" type="integer" label="Estimated genome size" value="10000000" help="Used only to control memory usage. e.g.3 billion (3000000000) uses 4Gb of RAM." />
        <param argument="-f" type="select" label="Process of search" help="Set the process of search in the graph" >
            <option value="1">Breadth</option>
            <option value="2">Depth</option>
        </param>
        <param argument="-x" type="integer" label="Max length of nodes" value="40" help="Set the maximal length of nodes"/>
        <param argument="-y" type="integer" label="Max depth of nodes" value="10000" help="Set the maximal depth of the graph"/>
    </inputs>

    <outputs>
        <data name="fasta" from_work_dir="res_k_*.fasta" format="fasta" label="Assembly with ${tool.name} on $on_string"/>
        <data name="coherent" from_work_dir="res_coherent_*.fasta" format="fasta" label="Coherent with ${tool.name} on $on_string" hidden="true"/>
        <data name="uncoherent" from_work_dir="res_uncoherent_*.fasta" format="fasta" label="Uncoherent with ${tool.name} on $on_string" hidden="true"/>
        <data name="extremities" from_work_dir="starter_extremities.fa" format="fasta" label="Starter extremities with ${tool.name} on $on_string" hidden="true"/>
    </outputs>

    <tests>
        <test>
            <param name="s" value="mapsembler2/starter.fa" />
            <param name="r" value="mapsembler2/reads1,mapsembler2/reads2" />
            <param name="t" value="2"/>
            <output name="fasta" file="mapsembler2/assembly.fa"/>
        </test>
        <test>
            <param name="s" value="mapsembler2/starter.fa" />
            <param name="r" value="mapsembler2/reads2,mapsembler2/reads1" />
            <param name="t" value="1"/>
            <output name="fasta" file="mapsembler2/assembly_2.fa"/>
            <output name="coherent" file="mapsembler2/coherent.fa"/>
            <output name="extremities" file="mapsembler2/starter_extremities.fa"/>
        </test>
    </tests>

    <help><![CDATA[

**Description**

Mapsembler2 is a targeted assembly software. It takes as input a set of NGS raw reads (fasta or fastq, gzipped or not) and a set of input sequences (starters). It first determines if each starter is read-coherent, e.g. whether reads confirm the presence of each starter in the original sequence. Then for each read-coherent starter, Mapsembler2 outputs its sequence neighborhood as a linear sequence or as a graph, depending on the user choice.
Mapsembler2 may be used for (not limited to):

· Validate an assembled sequence (input as starter), e.g. from a de Bruijn graph assembly where read-coherence was not enforced.

· Checks if a gene (input as starter) has an homolog in a set of reads.

· Checks if a known enzyme is present in a metagenomic NGS read set.

· Enrich unmappable reads by extending them, possibly making them mappable.

· Checks what happens at the extremities of a contig.

· Remove contaminants or symbiont reads from a read set

-------

**Web site**

http://colibread.inria.fr/mapsembler2/


    ]]></help>
    <expand macro="citations">
        <citation type="doi">10.1186/1471-2105-13-48</citation>
    </expand>

</tool>