comparison data_manager/bowtie_index_builder.py @ 1:35c1e3785c90 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/data_managers/data_manager_bowtie_index_builder commit 596a23abc0c657eff218d16df685b427ae7cdd0a

0:0a0c648498e2

#!/usr/bin/env python

import sys
import os
import tempfile
import optparse
import subprocess

from galaxy.util.json import from_json_string, to_json_string


CHUNK_SIZE = 2**20

DEFAULT_DATA_TABLE_NAME = "bowtie_indexes"

def get_id_name( params, dbkey, fasta_description=None):
    #TODO: ensure sequence_id is unique and does not already appear in location file
    sequence_id = params['param_dict']['sequence_id']
    if not sequence_id:
        sequence_id = dbkey

    sequence_name = params['param_dict']['sequence_name']

        if not sequence_name:
            sequence_name = dbkey
    return sequence_id, sequence_name


def build_bowtie_index( data_manager_dict, fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=DEFAULT_DATA_TABLE_NAME, color_space = False ):
    #TODO: allow multiple FASTA input files
    #tmp_dir = tempfile.mkdtemp( prefix='tmp-data-manager-bowtie-index-builder-' )
    fasta_base_name = os.path.split( fasta_filename )[-1]
    sym_linked_fasta_filename = os.path.join( target_directory, fasta_base_name )
    os.symlink( fasta_filename, sym_linked_fasta_filename )
    args = [ 'bowtie-build' ]
    if color_space:
        args.append( '-c' )
    args.append( sym_linked_fasta_filename)
    args.append( fasta_base_name )
    args.append( sym_linked_fasta_filename )
    tmp_stderr = tempfile.NamedTemporaryFile( prefix = "tmp-data-manager-bowtie-index-builder-stderr" )
    proc = subprocess.Popen( args=args, shell=False, cwd=target_directory, stderr=tmp_stderr.fileno() )
    return_code = proc.wait()
    if return_code:
        tmp_stderr.flush()
        tmp_stderr.seek(0)

    data_manager_dict['data_tables'][ data_table_name ].append( data_table_entry )
    return data_manager_dict


def main():
    #Parse Command Line
    parser = optparse.OptionParser()
    parser.add_option( '-f', '--fasta_filename', dest='fasta_filename', action='store', type="string", default=None, help='fasta_filename' )
    parser.add_option( '-d', '--fasta_dbkey', dest='fasta_dbkey', action='store', type="string", default=None, help='fasta_dbkey' )
    parser.add_option( '-t', '--fasta_description', dest='fasta_description', action='store', type="string", default=None, help='fasta_description' )
    parser.add_option( '-n', '--data_table_name', dest='data_table_name', action='store', type="string", default=None, help='data_table_name' )
    parser.add_option( '-c', '--color_space', dest='color_space', action='store_true', default=False, help='color_space' )
    (options, args) = parser.parse_args()

    filename = args[0]

    params = from_json_string( open( filename ).read() )
    target_directory = params[ 'output_data' ][0]['extra_files_path']
    os.mkdir( target_directory )
    data_manager_dict = {}

    dbkey = options.fasta_dbkey

    if dbkey in [ None, '', '?' ]:
        raise Exception( '"%s" is not a valid dbkey. You must specify a valid dbkey.' % ( dbkey ) )

    sequence_id, sequence_name = get_id_name( params, dbkey=dbkey, fasta_description=options.fasta_description )

    #build the index
    build_bowtie_index( data_manager_dict, options.fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=options.data_table_name or DEFAULT_DATA_TABLE_NAME, color_space=options.color_space )

    #save info to json file
    open( filename, 'wb' ).write( to_json_string( data_manager_dict ) )

if __name__ == "__main__":
    main()

1:35c1e3785c90

#!/usr/bin/env python
import json
import optparse
import os
import subprocess
import sys
import tempfile

CHUNK_SIZE = 2**20

DEFAULT_DATA_TABLE_NAME = "bowtie_indexes"


def get_id_name( params, dbkey, fasta_description=None):
    # TODO: ensure sequence_id is unique and does not already appear in location file
    sequence_id = params['param_dict']['sequence_id']
    if not sequence_id:
        sequence_id = dbkey

    sequence_name = params['param_dict']['sequence_name']

        if not sequence_name:
            sequence_name = dbkey
    return sequence_id, sequence_name


def build_bowtie_index( data_manager_dict, fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=DEFAULT_DATA_TABLE_NAME, color_space=False ):
    # TODO: allow multiple FASTA input files
    fasta_base_name = os.path.split( fasta_filename )[-1]
    sym_linked_fasta_filename = os.path.join( target_directory, fasta_base_name )
    os.symlink( fasta_filename, sym_linked_fasta_filename )
    args = [ 'bowtie-build' ]
    if color_space:
        args.append( '-c' )
    args.append( sym_linked_fasta_filename)
    args.append( fasta_base_name )
    args.append( sym_linked_fasta_filename )
    tmp_stderr = tempfile.NamedTemporaryFile( prefix="tmp-data-manager-bowtie-index-builder-stderr" )
    proc = subprocess.Popen( args=args, shell=False, cwd=target_directory, stderr=tmp_stderr.fileno() )
    return_code = proc.wait()
    if return_code:
        tmp_stderr.flush()
        tmp_stderr.seek(0)

    data_manager_dict['data_tables'][ data_table_name ].append( data_table_entry )
    return data_manager_dict


def main():
    # Parse Command Line
    parser = optparse.OptionParser()
    parser.add_option( '-f', '--fasta_filename', dest='fasta_filename', action='store', type="string", default=None, help='fasta_filename' )
    parser.add_option( '-d', '--fasta_dbkey', dest='fasta_dbkey', action='store', type="string", default=None, help='fasta_dbkey' )
    parser.add_option( '-t', '--fasta_description', dest='fasta_description', action='store', type="string", default=None, help='fasta_description' )
    parser.add_option( '-n', '--data_table_name', dest='data_table_name', action='store', type="string", default=None, help='data_table_name' )
    parser.add_option( '-c', '--color_space', dest='color_space', action='store_true', default=False, help='color_space' )
    (options, args) = parser.parse_args()

    filename = args[0]

    params = json.loads( open( filename ).read() )
    target_directory = params[ 'output_data' ][0]['extra_files_path']
    os.mkdir( target_directory )
    data_manager_dict = {}

    dbkey = options.fasta_dbkey

    if dbkey in [ None, '', '?' ]:
        raise Exception( '"%s" is not a valid dbkey. You must specify a valid dbkey.' % ( dbkey ) )

    sequence_id, sequence_name = get_id_name( params, dbkey=dbkey, fasta_description=options.fasta_description )

    # build the index
    build_bowtie_index( data_manager_dict, options.fasta_filename, params, target_directory, dbkey, sequence_id, sequence_name, data_table_name=options.data_table_name or DEFAULT_DATA_TABLE_NAME, color_space=options.color_space )

    # save info to json file
    open( filename, 'wb' ).write( json.dumps( data_manager_dict ) )

if __name__ == "__main__":
    main()