Mercurial > repos > iuc > dimet_differential_multigroup_analysis
diff dimet_differential_multigroup_analysis.xml @ 2:b581ea4908ae draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/DIMet commit 30fe10acdf65c6917856a0eae21dc91abd2f609f
author | iuc |
---|---|
date | Thu, 15 Feb 2024 12:55:12 +0000 |
parents | e88cf5618b40 |
children | efe13e94f70d |
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--- a/dimet_differential_multigroup_analysis.xml Tue Jan 23 14:58:10 2024 +0000 +++ b/dimet_differential_multigroup_analysis.xml Thu Feb 15 12:55:12 2024 +0000 @@ -11,10 +11,9 @@ <command detect_errors="exit_code"><![CDATA[ @INIT_CONFIG@ @INIT_DIFF_MULTIGROUP_ANALYSIS@ - @INIT_STAT_TEST@ @INIT_DATATYPES@ @INIT_GROUPS@ - @INIT_CONDITIONS@ + @INIT_PLOT_CONDITIONS@ @INIT_TIMEPOINTS@ @INIT_MULTIGROUP_COMPARISONS@ HYDRA_FULL_ERROR=1 python -m dimet @@ -36,16 +35,14 @@ datatypes:${datatypes_avail} }' '++analysis.method.datatypes=${datatypes_avail}' - '++analysis.method.qualityDistanceOverSpan='${qualityDistanceOverSpan}'' '++analysis.timepoints=${timepoints}' '++analysis.conditions=${comparisons}' '++analysis.dataset.label=' - '++analysis.statistical_test=${statistical_test}' '++analysis.method.correction_method=${correction_method}' '++analysis.method.impute_values=${impute_values}' '++analysis.dataset.subfolder=' '++analysis.method.grouping=${groups}' - '++analysis.dataset.conditions=${conds}' + '++analysis.dataset.conditions=${conditions}' #if $metadata_path: '++analysis.dataset.metadata=metadata' #end if @@ -69,12 +66,11 @@ @REMOVE_CONFIG@ ]]></command> <inputs> - <expand macro="input_parameters_diff_analysis"/> - <expand macro="conditions_multigroup"/> + <expand macro="input_parameters_multi_diff_analysis"/> + <expand macro="plot_factor_list"/> <expand macro="timepoint_multigroup"/> <expand macro="compartments"/> <expand macro="correction_method"/> - <param name="qualityDistanceOverSpan" type="float" min="-1.0" max="-0.1" value="-0.3" label="quality Distance Over Span" help="Default value is -0.3."/> </inputs> <outputs> @@ -87,10 +83,16 @@ <param name="data_input_selector" value="abundance" /> <param name="abundance_file" ftype="tabular" value="rawAbundances3.csv"/> <param name="metadata_path" ftype="tabular" value="example3_metadata.csv"/> - <param name="stat_test" value="Tt"/> <param name="correction_method" value="bonferroni"/> - <param name="qualityDistanceOverSpan" value="-0.3"/> - <param name="conditions" value="Control,Cond1,Cond2"/> + <repeat name="plot_factor_list"> + <param name="condition" value="Control"/> + </repeat> + <repeat name="plot_factor_list"> + <param name="condition" value="Cond1"/> + </repeat> + <repeat name="plot_factor_list"> + <param name="condition" value="Cond2"/> + </repeat> <param name="timepoint" value="T0h,T2h"/> <param name="compartments" value="cell"/> @@ -104,7 +106,7 @@ **Input data files** -This tool computes the Kruskall Wallis test over 3 or more groups, +This tool computes the Kruskal-Wallis test over 3 or more groups, to evaluate if at least one group is significantly different to the other groups (H0: median of all of the groups is equal). For illustration see the section **Metadata File Information** which contains three conditions: Control, Core_mass and Edge_tissue, across one single time point, thus 3 groups (number-of-groups-in-my-data = number-of-conditions x number-of-timepoints)). This tool requires (at max.) 5 tab-delimited .csv files as inputs. There are two types of files: @@ -247,11 +249,15 @@ There exist hints on use that will guide you, next to the parameters. +For more information about the implemented statistical tests, please visit: https://github.com/cbib/DIMet/wiki/2-Statistical-tests + +The output files are explained in https://github.com/cbib/DIMet/wiki/3-Output + **Available data for testing** -You can test our tool with the data from our manuscript https://zenodo.org/record/8378887 (the pertinent +You can test our tool with the data from our manuscript https://zenodo.org/record/10579862 (the pertinent files for you are located in the subfolders inside the data folder). -You can also use the minimal data examples from https://zenodo.org/record/8380706 +You can also use the minimal data examples from https://zenodo.org/record/10579891 ]]> </help>