view pyega3.xml @ 7:7d87a9d58aa1 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/pyega3 commit 9b235992a96145b852de7a6b59bac3a33dcbbcc8

<tool id="pyega3" name="EGA Download Client" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="21.01" >
    <macros>
        <token name="@TOOL_VERSION@">5.0.2</token>
        <token name="@VERSION_SUFFIX@">0</token>
    </macros>
    <requirements>
        <requirement type="package" version="@TOOL_VERSION@">pyega3</requirement>
    </requirements>
    <version_command><![CDATA[
        pyega3 -v |& grep version | cut -d" " -f 10
    ]]></version_command>
    <command detect_errors="exit_code"><![CDATA[
#set $username = $__user__.extra_preferences.get('ega_account|username', "")
#if $username == "":
    #set $username = "[e-mail] (default user)"
#end if
    echo "Running as user: $username. Set your credentials via: User -> Preferences -> Manage Information"  &&

#if $action.action_type == "list_datasets"
    pyega3 -cf '$credentials'
      datasets
#elif $action.action_type == "list_dataset_files"
    pyega3 -cf '$credentials'
      files '$action.dataset_id'
    &&

    ## create file header
    echo -e 'File ID\tStatus\tBytes\tCheck sum\tFile name' > '$dataset_file_list' &&

    ## remove timestamps and convert spaces to tabs
    grep EGAF pyega3_output.log | sed -e 's/^\[.*\]\s\+//g' | sed 's/\s\+/\t/g' >> '$dataset_file_list'

#elif $action.action_type == "download_file"
    pyega3 -c \${PYEGA_CONNECTIONS:-30} -cf '$credentials'
      fetch '$action.file_id'
      --max-retries 10
      #if $action.range.reference_name
        --reference-name '$action.range.reference_name'
        #if str($action.range.start)
          --start $action.range.start
        #end if
        #if $action.range.end
          --end $action.range.end
        #end if
      #end if
    && mv ${action.file_id} downloads
    && rm -f downloads/*.md5  ## checksum validation already performed by pyEGA, cleanup downloads folder

#elif $action.action_type == "download_files"
    #import re
    #set file_ids=[x.split('\t')[int(str($action.id_column))-1] for x in open(str($id_table)).readlines()]
    mkdir downloads
    #for f in $file_ids
      #if not f.startswith("EGAF")
        && >&2 echo "Ignoring \"$f\": no EGA file ID"
        #continue
      #end if
      && 
      echo 'Downloading $f'
      &&
      pyega3 -c \${PYEGA_CONNECTIONS:-30} -cf '$credentials'
        fetch '$f'
          --max-retries 10
          #if $action.range.reference_name
          --reference-name '$action.range.reference_name'
          #if str($action.range.start)
            --start $action.range.start
          #end if
          #if $action.range.end
            --end $action.range.end
          #end if
        #end if
        --output-dir downloads
    #end for
    && for vcf in \$(ls downloads/**/*vcf.gz); do mv "\${vcf}" "\${vcf:0:-6}vcf_bgzip"; done  ## renaming vcf.gz files to vcf_bgzip to recognize format
    && rm -f downloads/**/*.md5  ## checksum validation already performed by pyEGA, clean up downloads folder

#end if
    ]]></command>
    <configfiles>
        <configfile name="credentials"><![CDATA[
#set $password = $__user__.extra_preferences.get('ega_account|password', "")
#set $username = $__user__.extra_preferences.get('ega_account|username', "")
#if $username == "" or $password == "":
   #set $username = "[e-mail]"
   #set $password = "egarocks"
#end if
{
    "username": "$username",
    "password": "$password"
}
        ]]></configfile>
    </configfiles>
    <inputs>
        <conditional name="action">
            <param name="action_type" type="select" label="What would you like to do?">
                <option value="list_datasets"> List my authorized datasets </option>
                <option value="list_dataset_files"> List files in a datasets </option>
                <option value="download_file"> Download a file </option>
                <option value="download_files"> Download multiple files (based on a file with IDs) </option>
            </param>
            <when value="list_dataset_files">
                <param name="dataset_id" type="text" optional="false" label="EGA Dataset Accession ID" help="Identifier starting with 'EGAD'. For example: EGAD00001003338">
                    <validator type="regex" message="EGA dataset ID must be a string of numbers prefixed by 'EGAD'">EGAD[0-9]+</validator>
                </param>
            </when>
            <when value="list_datasets"/>
            <when value="download_file">
                <param name="file_id" type="text" optional="false" label="EGA File Accession Identifier" help="Identifier starting with 'EGAF'. For example: EGAF00001753735">
                     <validator type="regex" message="EGA Accession ID must be a string of numbers prefixed by 'EGAD' (datasets) or 'EGAF' (files)">EGA[DF][0-9]+</validator>
                </param>
                <section name="range" title="Request a specific Genomic range?" expanded="false">
                    <param argument="--reference-name" type="text" optional="true" label="Reference Sequence Name" help="For example 'chr1', '1', or 'chrX'. If unspecified, all data is returned." />
                    <param argument="--start" type="integer" optional="true" min="0" label="Start Position" help="0-based, inclusive. Only used if a reference sequence name was specified"/>
                    <param argument="--end" type="integer" optional="true" min="0" label="End Position" help="0-based, exclusive. Only used if a reference sequence name was specified"/>
                </section>
            </when>
            <when value="download_files">
                <param name="id_table" type="data" format="tabular" label="Table with IDs to download" help="A tabular file where one column contains the set of file IDs. This will output a collection. Please select files that are all the same format (e.g. all BAM or all VCF)."/>
                <param name="id_column" type="data_column" data_ref="id_table" label="Column containing the file IDs" help="File Identifiers starting with 'EGAF'. For example: EGAF00001753735" />
                <section name="range" title="Request a specific Genomic range? (will be applied to ALL requested files)" expanded="false">
                    <param argument="--reference-name" type="text" optional="true" label="Reference Sequence Name" help="For example 'chr1', '1', or 'chrX'. If unspecified, all data is returned." />
                    <param argument="--start" type="integer" optional="true" min="0" label="Start Position" help="0-based, inclusive. Only used if a reference sequence name was specified"/>
                    <param argument="--end" type="integer" optional="true" min="0" label="End Position" help="0-based, exclusive. Only used if a reference sequence name was specified"/>
                </section>
            </when>
        </conditional>
        <param name="output_log" type="boolean" checked="false" label="Output the log file?"/>
    </inputs>
    <outputs>
        <data name="authorized_datasets" format="txt" from_work_dir="pyega3_output.log" label="${tool.name}: authorized datasets">
            <filter> action['action_type'] == 'list_datasets' </filter>
        </data>
        <data name="dataset_file_list" format="tabular" label="${tool.name}: ${action.dataset_id} - file list">
            <filter> action['action_type'] == 'list_dataset_files' </filter>
        </data>
        <data name="downloaded_file" auto_format="true" from_work_dir="downloads/*" label="${tool.name}: ${action.file_id} ${action.range.reference_name} ${action.range.start} ${action.range.end}">
            <filter> action['action_type'] == 'download_file' </filter>
        </data>
        <data name="logfile" format="txt" from_work_dir="pyega3_output.log" label="${tool.name}: log">
            <filter> output_log </filter>
        </data>
        <collection name="downloaded_file_collection" type="list" label="${tool.name} on ${on_string}: Downloaded datasets">
            <filter> action['action_type'] == 'download_files' </filter>
            <discover_datasets pattern="__name_and_ext__" recurse="true" directory="downloads" />
        </collection>
    </outputs>
    <tests>
        <!-- list datasets with default credentials -->
        <test expect_num_outputs="1">
            <param name="action_type" value="list_datasets"/>
            <output name="authorized_datasets" ftype="txt">
                <assert_contents>
                    <has_text text="pyEGA3 - EGA python client version @TOOL_VERSION@"/>
                    <has_text text="EGAD00001003338"/>
                </assert_contents>
            </output>
        </test>
        <!-- list dataset files with default credentials, and request a log output file -->
        <test expect_num_outputs="2">
            <param name="action_type" value="list_dataset_files"/>
            <param name="dataset_id" value="EGAD00001003338"/>
            <param name="output_log" value="true"/>
            <output name="dataset_file_list" file="filelist_EGAD00001003338.tabular"/>
            <output name="logfile" ftype="txt">
                <assert_contents>
                    <has_text text="pyEGA3 - EGA python client version @TOOL_VERSION@"/>
                    <has_line_matching expression="^\[.*\]\s+File ID\s+Status\s+Bytes\s+Check sum\s+File name$"/>
                    <has_text text="EGAF00001753734"/>
                </assert_contents>
            </output>
        </test>
        <!-- download a single file -->
        <test expect_num_outputs="1"> 
            <param name="action_type" value="download_file"/>
            <param name="file_id" value="EGAF00001775036"/>
            <output name="downloaded_file" md5="3b89b96387db5199fef6ba613f70e27c"/>
        </test>
        <!-- download a single vcf.gz -->
        <test expect_num_outputs="1"> 
            <param name="action_type" value="download_file"/>
            <param name="file_id" value="EGAF00007243775"/>
            <output name="downloaded_file" md5="51cfb69bf3b9416ff425381a58c18a2b" ftype="vcf_bgzip" />
        </test>
        <!-- download a single bam, with genomic range specified -->
        <test expect_num_outputs="1">
            <param name="action_type" value="download_file"/>
            <param name="file_id" value="EGAF00001753756"/>
            <param name="reference_name" value="1"/>
            <param name="start" value="0"/>
            <param name="end" value="10000"/>
            <output name="downloaded_file" md5="4b5344ad094c2bed7787430259205098"/>
        </test>
        <!-- download multiple files -->
        <test expect_num_outputs="1">
            <param name="action_type" value="download_files"/>
            <param name="id_table" value="filelist.tabular"/>
            <param name="id_column" value="1"/>
            <output_collection name="downloaded_file_collection" type="list" count="2">
                <element name="ENCFF000VWO.bam" md5="b8ae14d5d1f717ab17d45e8fc36946a0" />
                <element name="ENCFF284YOU.bam" md5="3b89b96387db5199fef6ba613f70e27c" />
            </output_collection>
            <assert_stderr>
                <has_text text="Ignoring &quot;File ID&quot;: no EGA file ID"/>
            </assert_stderr>
        </test>
        <!-- download multiple files, in combination with a genomic range -->
        <test expect_num_outputs="1">
            <param name="action_type" value="download_files"/>
            <param name="id_table" value="filelist2.tabular"/>
            <param name="id_column" value="1"/>
            <param name="reference_name" value="1"/>
            <param name="start" value="0"/>
            <param name="end" value="10000"/>
            <output_collection name="downloaded_file_collection" count="2">
                <element name="NA19239_genomic_range_1_0_10000" md5="497f443830a6a3ec2e5bb5a19c4db638" />
                <element name="NA19240_genomic_range_1_0_10000" md5="4b5344ad094c2bed7787430259205098" />
            </output_collection>
            <assert_stderr>
                <has_text text="Ignoring &quot;File ID&quot;: no EGA file ID"/>
            </assert_stderr>
        </test>
        <!-- download multiple vcf.gz files -->
        <test expect_num_outputs="1">
            <param name="action_type" value="download_files"/>
            <param name="id_table" value="filelist3.tabular"/>
            <param name="id_column" value="1"/>
            <output_collection name="downloaded_file_collection" type="list" count="2">
                <element name="HG00408.novoBreak__256r__4.100100-10100100__7.200100-9000100" md5="51cfb69bf3b9416ff425381a58c18a2b" ftype="vcf_bgzip" />
                <element name="HG01890.HGSVC__145r__1.900100-10001000__18.2001000-90001000" md5="ebad4425191a89d3e970c02190a87175" ftype="vcf_bgzip" />
            </output_collection>
        </test>
    </tests>
    <help><![CDATA[
The pyEGA3 download client is a python-based tool for viewing and downloading files from authorized EGA datasets.

.. class:: Warning mark

	Data is stored unencrypted on the user's Galaxy account. Confidential data from the EGA could be left unprotected when uploaded to a public Galaxy server.
	Make sure to read the EGA Data Access Agreement (DAA_) before uploading any data to Galaxy from the EGA.
	If this applies to you, we recommend you follow the GalaxySensitiveData-ELIXIR-IS_ page for updates on encrypted data at rest.

If you have an EGA account, you can set your EGA credentials in the user preferences menu of Galaxy. Otherwise, default EGA credentials with access to an example dataset will be used.

pyEGA3 uses the EGA Data API and has several key features:

- Files are transferred over secure https connections and received unencrypted, so no need for decryption after download.
- Downloads resume from where they left off in the event that the connection is interrupted.
- pyEGA3 supports file segmenting and parallelized download of segments, improving overall performance.
- After download completes, file integrity is verified using checksums.
- pyEGA3 implements the GA4GH-compliant htsget protocol for download of genomic ranges for data files with accompanying index files.

.. _DAA: https://ega-archive.org/files/Example_DAA.doc
.. _GalaxySensitiveData-ELIXIR-IS: https://github.com/elixir-europe/GalaxySensitiveData-ELIXIR_IS
    ]]></help>
    <citations>
        <citation type="doi">10.1038/ng.3312</citation>
    </citations>
</tool>