Mercurial > repos > iuc > emboss_needle
changeset 0:2f0dc62d0a19 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/emboss commit 08f38b1f9b4241ba9037c64f732621efc628fd43
| author | iuc |
|---|---|
| date | Mon, 20 Jan 2025 16:22:05 +0000 |
| parents | |
| children | |
| files | emboss_needle.xml macros.xml test-data/1.fasta test-data/2.fasta test-data/emboss_needle_out.fasta test-data/emboss_needle_out.markx10 test-data/emboss_needle_out.score test-data/emboss_needleall_input1.fa test-data/emboss_needleall_input2.fq test-data/emboss_needleall_out.fasta test-data/emboss_needleall_out.pair test-data/emboss_needleall_out.score |
| diffstat | 12 files changed, 24299 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/emboss_needle.xml Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,143 @@ +<tool id="emboss_needle" name="EMBOSS: needle" version="@VERSION@+galaxy0" profile="@PROFILE@"> + <description>Needleman-Wunsch global alignment</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="bio_tools" /> + <expand macro="requirements" /> + <version_command>needle -version</version_command> + <command detect_errors="exit_code"><![CDATA[ +needle -asequence '$asequence' +-bsequence '$bsequence' +-outfile '$out_file1' +-gapopen $gapopen +-gapextend $gapextend +-brief $brief +-aformat3 $out_format1 +-auto +#if $datafile +-datafile $datafile +#end if +#if $endgap.endweight == 'yes' +-endopen $endgap.endopen +-endextend $endgap.endextend +#end if +]]></command> + <inputs> + <param argument="-asequence" type="data" format="fasta,fastq" label="Sequence 1" /> + <param argument="-bsequence" type="data" format="fasta,fastq" label="Sequence 2" /> + + <expand macro="scoring_matrix"/> + <expand macro="gap_penalties"/> + <expand macro="endgap_penalties"/> + <expand macro="param_brief"/> + + <expand macro="choose_alignment_output_format"/> + </inputs> + <outputs> + <data name="out_file1" format="needle" label="${tool.name} on ${on_string}: alignment output" > + <expand macro="change_alignment_output_format"/> + </data> + </outputs> + <tests> + <test> + <param name="asequence" value="2.fasta"/> + <param name="bsequence" value="1.fasta"/> + <param name="gapopen" value="10"/> + <param name="gapextend" value="0.5"/> + <param name="brief" value="yes"/> + <param name="out_format1" value="score"/> + <output name="out_file1" file="emboss_needle_out.score" ftype="score"/> + </test> + <test><!--test with fastq input --> + <param name="asequence" value="emboss_needleall_input2.fq"/> + <param name="bsequence" value="1.fasta"/> + <param name="gapopen" value="10"/> + <param name="gapextend" value="0.5"/> + <param name="brief" value="yes"/> + <param name="out_format1" value="markx10"/> + <output name="out_file1" file="emboss_needle_out.markx10" ftype="markx10" lines_diff="10"/> + </test> + <test><!-- test with fasta output, custom matrix, and endgap penalties --> + <param name="asequence" value="2.fasta"/> + <param name="bsequence" value="1.fasta"/> + <param name="gapopen" value="10"/> + <param name="gapextend" value="0.5"/> + <param name="datafile" value="EPAM30"/> + <conditional name="endgap"> + <param name="endweight" value="yes"/> + <param name="endopen" value="13.37"/> + <param name="endextend" value="2.5"/> + </conditional> + <param name="brief" value="yes"/> + <param name="out_format1" value="fasta"/> + <output name="out_file1" file="emboss_needle_out.fasta" ftype="fasta"/> + </test> + </tests> + <help><![CDATA[ + +needle reads any two sequences of the same type (DNA or protein). + +This tool uses the Needleman-Wunsch global alignment algorithm to find the optimum alignment (including gaps) of two sequences when considering their entire length. + +- **Optimal alignment:** Dynamic programming methods ensure the optimal global alignment by exploring all possible alignments and choosing the best. + +- **The Needleman-Wunsch algorithm** is a member of the class of algorithms that can calculate the best score and alignment in the order of mn steps, (where 'n' and 'm' are the lengths of the two sequences). + +- **Gap open penalty:** [10.0 for any sequence] The gap open penalty is the score taken away when a gap is created. The best value depends on the choice of comparison matrix. The default value assumes you are using the EBLOSUM62 matrix for protein sequences, and the EDNAFULL matrix for nucleotide sequences. (Floating point number from 1.0 to 100.0) + +- **Gap extension penalty:** [0.5 for any sequence] The gap extension, penalty is added to the standard gap penalty for each base or residue in the gap. This is how long gaps are penalized. Usually you will expect a few long gaps rather than many short gaps, so the gap extension penalty should be lower than the gap penalty. An exception is where one or both sequences are single reads with possible sequencing errors in which case you would expect many single base gaps. You can get this result by setting the gap open penalty to zero (or very low) and using the gap extension penalty to control gap scoring. (Floating point number from 0.0 to 10.0) + +You can view the original documentation here_. + + .. _here: http://galaxy-iuc.github.io/emboss-5.0-docs/needle.html + +----- + +**Example** + +- Input File:: + + >hg18_dna range=chrX:151073054-151073136 5'pad=0 3'pad=0 revComp=FALSE strand=? repeatMasking=none + TTTATGTCTATAATCCTTACCAAAAGTTACCTTGGAATAAGAAGAAGTCA + GTAAAAAGAAGGCTGTTGTTCCGTGAAATACTG + +- If both Sequence1 and Sequence2 take the above file as input, Gap open penalty equals 10.0, Gap extension penalty equals 0.5, Brief identity and similarity is set to Yes, Output alignment file format is set to SRS pairs, the output file is:: + + ######################################## + # Program: needle + # Rundate: Mon Apr 02 2007 14:23:16 + # Align_format: srspair + # Report_file: ./database/files/dataset_7.dat + ######################################## + + #======================================= + # + # Aligned_sequences: 2 + # 1: hg18_dna + # 2: hg18_dna + # Matrix: EDNAFULL + # Gap_penalty: 10.0 + # Extend_penalty: 0.5 + # + # Length: 83 + # Identity: 83/83 (100.0%) + # Similarity: 83/83 (100.0%) + # Gaps: 0/83 ( 0.0%) + # Score: 415.0 + # + #======================================= + + hg18_dna 1 TTTATGTCTATAATCCTTACCAAAAGTTACCTTGGAATAAGAAGAAGTCA 50 + |||||||||||||||||||||||||||||||||||||||||||||||||| + hg18_dna 1 TTTATGTCTATAATCCTTACCAAAAGTTACCTTGGAATAAGAAGAAGTCA 50 + + hg18_dna 51 GTAAAAAGAAGGCTGTTGTTCCGTGAAATACTG 83 + ||||||||||||||||||||||||||||||||| + hg18_dna 51 GTAAAAAGAAGGCTGTTGTTCCGTGAAATACTG 83 + + #--------------------------------------- + #--------------------------------------- + ]]></help> + <expand macro="citations" /> +</tool>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,159 @@ +<macros> + <token name="@PROFILE@">23.1</token> + <token name="@VERSION@">6.6.0</token> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@VERSION@">emboss</requirement> + <requirement type="package" version="5.26">perl</requirement> + </requirements> + </xml> + <xml name="bio_tools"> + <xrefs> + <xref type="bio.tools">emboss</xref> + </xrefs> + </xml> + <xml name="citations"> + <citations> + <citation type="doi">10.1016/S0168-9525(00)02024-2</citation> + <citation type="doi">10.1101/gr.5578007</citation> + <yield /> + </citations> + </xml> + + <xml name="scoring_matrix"> + <param argument="-datafile" type="select" optional="true" label="Scoring matrix" help="This is the scoring matrix used when comparing sequences. By default it is EBLOSUM62 (for proteins) or EDNAFULL (for nucleic sequences)"> + <option value="EBLOSUM30">EBLOSUM30</option> + <option value="EBLOSUM35">EBLOSUM35</option> + <option value="EBLOSUM40">EBLOSUM40</option> + <option value="EBLOSUM45">EBLOSUM45</option> + <option value="EBLOSUM50">EBLOSUM50</option> + <option value="EBLOSUM55">EBLOSUM55</option> + <option value="EBLOSUM60">EBLOSUM60</option> + <option value="EBLOSUM62">EBLOSUM62</option> + <option value="EBLOSUM62-12">EBLOSUM62-12</option> + <option value="EBLOSUM65">EBLOSUM65</option> + <option value="EBLOSUM70">EBLOSUM70</option> + <option value="EBLOSUM75">EBLOSUM75</option> + <option value="EBLOSUM80">EBLOSUM80</option> + <option value="EBLOSUM85">EBLOSUM85</option> + <option value="EBLOSUM90">EBLOSUM90</option> + <option value="EBLOSUMN">EBLOSUMN</option> + <option value="EDNAFULL">EDNAFULL</option> + <option value="EDNAMAT">EDNAMAT</option> + <option value="EDNASIMPLE">EDNASIMPLE</option> + <option value="ENUC.4.2">ENUC.4.2</option> + <option value="ENUC.4.4">ENUC.4.4</option> + <option value="EPAM10">EPAM10</option> + <option value="EPAM100">EPAM100</option> + <option value="EPAM110">EPAM110</option> + <option value="EPAM120">EPAM120</option> + <option value="EPAM130">EPAM130</option> + <option value="EPAM140">EPAM140</option> + <option value="EPAM150">EPAM150</option> + <option value="EPAM160">EPAM160</option> + <option value="EPAM170">EPAM170</option> + <option value="EPAM180">EPAM180</option> + <option value="EPAM190">EPAM190</option> + <option value="EPAM20">EPAM20</option> + <option value="EPAM200">EPAM200</option> + <option value="EPAM210">EPAM210</option> + <option value="EPAM220">EPAM220</option> + <option value="EPAM230">EPAM230</option> + <option value="EPAM240">EPAM240</option> + <option value="EPAM250">EPAM250</option> + <option value="EPAM260">EPAM260</option> + <option value="EPAM270">EPAM270</option> + <option value="EPAM280">EPAM280</option> + <option value="EPAM290">EPAM290</option> + <option value="EPAM30">EPAM30</option> + <option value="EPAM300">EPAM300</option> + <option value="EPAM310">EPAM310</option> + <option value="EPAM320">EPAM320</option> + <option value="EPAM330">EPAM330</option> + <option value="EPAM340">EPAM340</option> + <option value="EPAM350">EPAM350</option> + <option value="EPAM360">EPAM360</option> + <option value="EPAM370">EPAM370</option> + <option value="EPAM380">EPAM380</option> + <option value="EPAM390">EPAM390</option> + <option value="EPAM40">EPAM40</option> + <option value="EPAM400">EPAM400</option> + <option value="EPAM410">EPAM410</option> + <option value="EPAM420">EPAM420</option> + <option value="EPAM430">EPAM430</option> + <option value="EPAM440">EPAM440</option> + <option value="EPAM450">EPAM450</option> + <option value="EPAM460">EPAM460</option> + <option value="EPAM470">EPAM470</option> + <option value="EPAM480">EPAM480</option> + <option value="EPAM490">EPAM490</option> + <option value="EPAM50">EPAM50</option> + <option value="EPAM500">EPAM500</option> + <option value="EPAM60">EPAM60</option> + <option value="EPAM70">EPAM70</option> + <option value="EPAM80">EPAM80</option> + <option value="EPAM90">EPAM90</option> + <option value="SSSUB">SSSUB</option> + </param> + </xml> + + <xml name="param_brief"> + <param argument="-brief" type="select" label="Brief identity and similarity"> + <option value="yes">Yes</option> + <option value="no">No</option> + </param> + </xml> + + <xml name="gap_penalties"> + <param argument="-gapopen" type="float" value="10.0" min="1.0" max="100.0" label="Gap open penalty" /> + <param argument="-gapextend" type="float" value="0.5" min="0.0" max="10.0" label="Gap extension penalty" /> + </xml> + + <xml name="endgap_penalties"> + <conditional name="endgap"> + <param argument="-endweight" type="select" label="Apply end gap penalties?" help=""> + <option value="yes">yes</option> + <option value="no" selected="true">no</option> + </param> + <when value="yes"> + <param argument="-endopen" type="float" value="10.0" min="0.0" max="100.0" label="Penalty for creation of and end gap." help="The best value depends on the choice of comparison matrix. The default value assumes you are using the EBLOSUM62 matrix for protein sequences, and the EDNAFULL matrix for nucleotide sequences."/> + <param argument="-endextend" type="float" value="0.5" min="0.0" max="10.0" label="The end gap extensionpenalty" help="this penalty is added to the end gap penalty for each base or residue in the end gap."/> + </when> + <when value="no"/> + </conditional> + </xml> + + <xml name="choose_alignment_output_format"> + <param name="out_format1" type="select" label="Output alignment file format"> + <option value="srspair">SRS pair (p)</option> + <option value="simple">Simple (m)</option> + <option value="fasta">FASTA (m)</option> + <option value="msf">MSF (m)</option> + <option value="srs">SRS (m)</option> + <option value="pair">Pair (p)</option> + <option value="markx0">Markx0 (p)</option> + <option value="markx1">Markx1 (p)</option> + <option value="markx2">Markx2 (p)</option> + <option value="markx3">Markx3 (p)</option> + <option value="markx10">Markx10 (p)</option> + <option value="score">Score (p)</option> + </param> + </xml> + + <xml name="change_alignment_output_format"> + <change_format> + <when input="out_format1" value="fasta" format="fasta"/> + <when input="out_format1" value="score" format="score"/> + <when input="out_format1" value="pair" format="pair"/> + <when input="out_format1" value="srspair" format="srspair"/> + <when input="out_format1" value="simple" format="simple"/> + <when input="out_format1" value="srs" format="srs"/> + <when input="out_format1" value="msf" format="msf"/> + <when input="out_format1" value="markx0" format="markx0"/> + <when input="out_format1" value="markx1" format="markx1"/> + <when input="out_format1" value="markx2" format="markx2"/> + <when input="out_format1" value="markx3" format="markx3"/> + <when input="out_format1" value="markx10" format="markx10"/> + </change_format> + </xml> +</macros>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/1.fasta Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,2 @@ +>hg17 +gtttgccatcttttgctgctctagggaatccagcagctgtcaccatgtaaacaagcccaggctagaccaGTTACCCTCATCATCTTAGCTGATAGCCAGCCAGCCACCACAGGCAtgagtcaggccatattgctggacccacagaattatgagctaaataaatagtcttgggttaagccactaagttttaggcatagtgtgttatgtaTCTCACAAACATATAAGACTGTGTGTTTGTTGACTGGAGGAAGAGATGCTATAAAGACCACCTTTTAAAACTTCCC-------------------------------AAATACT-GCCACTGATGTCCTG-----ATGGAGGTA-------TGAA-------------------AACATCCACTAAAATTTGTGGTTTATTCATTTTTCATTATTTTGTTTAAGGAGGTCTATAGTGGAAGAGGGAGATATTTGGggaaatt---ttgtatagactagctttcacgatgttagggaattattattgtgtgataatggtcttgcagttaca-cagaaattcttccttattttttgggaa---gcaccaaag----tagggat---aaaatgtcatgatgtgtgcaatacactttaaaatgtttttg-----ccaaaataatt----------------aatgaagc--aaatatggaaa-ataataattattaaatctaggtgatgggtatattgtagttcactatagtattgcacacttttctgtatgtttaaatttttcatttaaaaaaaaactttgagc-----tagacaccaggctatgagctaggagcatagcaatgaccaa----------------------------------------------------------------------------------------------atagactcctaccaa--------------------------------------------------ctc-aaagaatgcacattctCTGGGAAACATGTTTCCATTAGGAAGCCTCGAATGCAATGTGACTGTGGTCTCCAGGACCTG-TGTGATCCTGGCTTTTCCTGTTCCCTCCG---CATCATCACTGCAGGTGTGTTTTCCCAAGTTTTAAACATTTA------CCTTCCCAGTGGCCTTGCGTCTAGAGGAATCCCTGTATAGTGGT-ACATGAATATAACACATAACAAA-AATCATCTCTATGGTGTGTGTTGTTCCTGGGGTTCAattcagcaaattttccctg-ggcacccatgtgttcttggcactggaaaagtaccgggactgaaacagttgatggccca-atccctgtcctct---taaaacctaagggaggagaTGGAAAGGGG-CACCCAACCCAGACTGAGAGACAGGAATTAGCTGCAAGGGGAACTAGGAAAAGCTTCTTTA---AGGATGGAGAGGCCCTAGTGGAAT-GGGGAGATTCTTCCGGGAGAAGCGATGGATGCACAGTTGGGCATCCCCACAGACGGACTGGAAAGAAAAAAGGCCTGGAGGAATCAATGTG-------CAATGTATGTGTGTTCCCTGGTTcaagggctgg-gaactttctcta-aagggccaggtagaaaacattttaggctttctaagccaag--gcaaaat-tgaggatattacatgggtacttatacaacaagaataaacaatt---tacacaattttttgttgacagaattcaaaactttat----agacacagaaatgcaaatttcctgtaattttcccat-gagaactattcttct--tttgttttgttttgcgacAGGGTTGCGCtgatcctcccgcctcagtctccctaagtgctgagatgttgcaggaagtcagggaccccgaacagagagatcggctggagccgtggcagaggaacataaattttgaagatttcattttaatatggacacttatcagttcccaaataatacttttataattttttatgcctgtctttgctttaatctcttaatcctgttatcttcataagctaaggatgtacgtcacctcaggaccactgtgataattgtgttaactgtacagattgattgcaaaacatgtgtgtttgaacaatatgaaatcagtgcaccttgaaaaagagcagaataacagcaatttttagggaacaagggaagacaactataaggtctgactgcctgcggggtcgggcaaagggagccatatttttcttcttgcagagagcctataaatagacctgcaagtaggagagatattgctaatttcttttgctagcatggaatattaatattaacaccctgggaaaggaatgcattcctggggggaggtctataaatggccgctctgggaatgtctatcctacgcaatggagataaggactgagatacgccctggtctcctgcagtaccctcaggcttactagggtggtgaaaaactccgccctggtaaatttgtggtcagaccagttttctgctctcgaacactgttttctgttgtttaagatgtttatcaagacaatacgtgcaccgctgaacacagacccttatcagtagttctcctttttgccctttgaagcatgtgatctactccctgttttacaccccctcaccttttgaaacccttaataaaaaacttgctggttt-gaggctcaggtgggcatcacagtactaccgatatgtgatgtcacccccggcggcccagctgtaaaattcctctctttgtactctctctctttatttctcagccagctgacacttatggaaaatagaaagaacctacgttgaaatattgggggcaggttcccccaataTCTGGTGCCCAACGTGGGAtactgagattacaagcatgagccactgcatctggcctcttcttttgatttttttttttcaaacttttacaaatgtagaaaccattcttagcttttgggcattaccaaacccggcagtgg-caggctcggttcaccaacgtcatttgcagttccccgCTTTATGTTATGGgttttgttttgttttgtttttttt-attgagacagagtttcactcttgttgcccaggctgtagtgcaatggtctgatcttggctcactgcaacctccacttcccaggttcaagccattctcctgcctcagcctctcaagtagctgggattacagacactcaccaccacacctggctaattttgtatttttagtagagatgaggtttcaccatgttggccaggctggtctcgaaatcctgacctcaggtgatccacccaccttggcctcccaaagtgctgggattacaggcttgagctaccacgcctggctGGGTTGGTTCTCAATGGAGTGGTTTGTTTTTGGAGCTGCTCT-GCGCAGtggggaccagaataggcctg-------------------ggttcctagcccattgctattcctt----accagctgtggattctaaggaaagtcatttaacctcgctggaccttag-attcctcatccctgaaGCCCAAGGGTaaaacaaaacaaaacaaaacaaaacaaaccaaCCCATCATGTAAAGCGGGGAACTACAAACGATACAGGTGAAACATGCCTACCACACCACTCACAGGCT--ATGATGACAAAAACGTGGCTACATCTGGGACCACCCCCCAACCCCCACTTTGTACGTAGGAAATACGGAGTTGAGGATGGAGACCCACAGTATGTCCAGAGTGTCCCCAAAGGCCACAGTGCCCGCCTGGAGCCCTCCAGAGAGCGTGCACTCCCTGGGGTGCCAGCCAGAGACAACTTGCCCTGAGGCTTGGAACTCGATTCTCCGCGTGCCAGAGAAGGGGTGGGACTTCAGAACCCCCAACCCCGCAATCTGGGTCGGGGAGCCTGGCGCACTGCGGGCCGCTCCCTCTAACCCTGGGCTTCCCTG------GCGTCCAGGGCCGTCGG-----------GGCCGAGTCCCGATTCGCTCCCACCCCGAAGCCGCGCCAGGACCAACGAGGGCGCAGCCGTATGCCCCAGCCCGCTCCGCGGAGCCCCTCACAGCCAcccccgccccgaccgcgccccgcgcggcTCGAAGCACCTTCCCAAGGGGCTGGTCCTTGC----------GCCATAGTCGCGCCGGAGCCTCTGGAGGGACATCAAGGATTTCTC-GCTCCTACCAGCCACCCCCAAATTTTTGGGAGGTACCCAAGGGTGCGCGCGTGGCTCCTGGCGCGCCGAGGCCCTCCCTCGAGGCCCCGCGAGGTGCACACTGC---------GGGCCCAGGGCTAGCAGCCGCCCGGCACGTCGCTACCCTGAGGGGCGGGGCGGGAGCTGGCGCTAGAAATGCGCCGGGGCCTGCGGGGCAGTTGCGCAAGTTGTGATCGGGCCGCTATAAGAGGGGCGGGCAGGCATGGAGCCCCGTAGGAATCGCAGCGCCAGCGGTTGCAAGGTAAGGCCC-CGGCGCGCTCCTTCCTCCTTCTCTGCTGGTCTTTCTTGGCAGGCCACAGGGCCCCACACAACTCTGGATCCCGGGGAAACTGAGTCAGG-AGGGATGCAGGGCGGATGGCTTAGTTCTGGACTATGATAGCTTTGTACCGAG-----TTCTAGCCAGATAGAAGGTTACCGGGAGCTGGGGAGCGTTGGATTTGCTGCTGGGCTGTGCCGGTGCCCAGAAGGCA------GGACCTTGCAGAACCAGCCAGGTCCCTGGGAGACTGTCAGACCCACCAACCTGGTGGCATTCGCAGAGCTGAGATGCATTGGAAATTGCCTTGGGCACATCCCCAAAGATCAGGATGTCCCACCCCAGTCTGAAGGAGA---TAAAGTTGGGGGTAGGAGAGACGCAG-ATGCAAGTGATCAGTCTC---AGTCCCAGACATTGCCTTGCTCTGCGGGTAGGAATTCAGGATTCATTTTCCAGGGAAG--------TTCCTGACCTCTGAATGAGAGGGGCTGTGTAAGGCCAATGCCTGGG-AGGAAGGCAAGGATGAGTAGAGGTGGGGGGAAACAAGTGTCAGGAAGA--------------------------------------------CTCAAA---------ATCTTC--------------------------------------------------------------CAGAGAAATTGT-----GCAGGGTCTTACCAGATCTGTCCTCAAAGCCATGCAAATTGCCTTCTTTGCAATGCAT-ACAATGAGGTGTCTCTGGGGGTCAGAACTGG-----------------------TTATTAGGGAACTTCTAGCCAGGACTGCTAAATACGCGCTGTTGG---------CCCACCAGGCTCACCTATAGCCT-TCCTTCAGTCTGGGCTTGGTTTGGATTTCACTGTGGGTGCCATCGCCTTTACACTCCTGTTTCTATAGTTTAAAGATAGTGGTGCTTTGGGAAAG---TGACTCCTTAAATACAGTTAGGTCCAAGTGA-GACAAGTGGCCTGGCTGTCATTTCAGAATAGCAGCTTCCAAGAGG----------TGATTAATTTCTGTTGGAAGGGTGAT-CTTTGGGGAGGT--GGGTGAAGAGCAGAGACTTGGTGGTACCGTTCCAGGAGCACAGGCTCTCT-----TCCTTTGCA--GTGCAGAATGACCTCTGGCAGCCGGAGTTGTGTTTGTT--------CTGTAGGATTCTGAGGTGGGCCATGGGCAGCTGGAACTGGG-----GAATTTTGCCAATCTCTTTCATATTAGGATTGTCTGCAGAACCAGATATGGAGG------CTTCTAGCAACGTGAGTGCTCCTGTTCTAATGCCCTTAGAAACAAGAAGGCCACACTGATCATTTCTCTCACTTAGGCAGGGAGACAAGGCAAGAGAGAAACAGT-----------------GGATGC--TTTTAGGTTCTTTCCCTTCCCAAGCAGTTGTGGACATTGGGCTGA-GGGGAACATTTCCACATTGGCTAAAGGAGCGTCCTCCTCATATTTTGTACATTTTATACCCAA--AATAA-CTCTTCTTGGTATTT-GGGGAAATATTTTCCTCCCCGTCC------------ATTCCAGGAAATGGCTCCAAGTGCCAAGGACAGAGCCAGGGAAGTTGCAATGAATTCCTGCCCGTCAGCCCCAGGCAGATGCCTTGCACGTCTGAGTGGCCCATGCAGAGCGTGGAGGTGGCCGCC----------------ACGGAACC-TGGGTCAATGT-CCCACCCCCG----CTTAGATGCCA-CCAGGGG--CGTGGGAGCCAAGGAG--AGAAGAGGGGCTCCAGGAAGGTAGAGTCCTTGTGTCTTGTGCATCTGTGAACAGCACTGGTATGATTTAAAGGAAAATTGAGCCAAATTTTCCGGCAGTCAGTT-----ACCCCATCCCCACCGGGGTAGGAGTCTGGCAGCCGCAGCTCCATTCTGGCCAGTCGGCAGAGAGCCTTGAAATTCTTCTTTGTCCACACAGTTGTCTCAGAGAAACAG--AGAGGTT-GTTTCTGCTTAAAAACAACACACTTGGTGTCTGGGCCCACAGACTCCTTTGCACTTATTCCACGTGTGACAGCCAATGTGCCTCGTTGCTTAGCAGACAGCATGTTACCGTCTTTCCTGCTCAGTTTGTTAG--------------CTCTATGGAATGGAATTTATAATCAATGCCCATACCAACATTTCACTAATATCATAGGAGATTTAGTCTCCATCTGGGTGTACATTACATTTGC--TCTGGGG-TGCTCCAGGC--TGGGGGGTTGCCAAGGAAGAGAAGAGAAACCGCAGAGAAGAC---GGGAGGGCAGGGCAGGGGTCTCTGAGAAGGGGAGGGGTCCCAGAGTGCAGGAGCAGGAGCCAGGCTC---------ATGAAAGGGGCCACGGGCGGGAGTATCCAGGGACGGCAGTCAAGATGGAGCACAGCTTAGG--AAGCTGAAGGGAATCCTGGCCCACCTGGGTGCTAGAGGGCACATAGGAAGTGCAGGAAGCAGACCAAGGTCCCCAAGAGAGGGAGACCTGGACGCTGAAGCATTTTCTGTCTTTATTAAG-------------ACAACTCCGTAAGAATTCCTGCTGGGCCAAAGTGAATTCTAGGATGCGACTTTAAGATGGGAGCAAGCGAACCATTGAGGAGGCAGGTTACCCTAGTTAGCCAATGCAGATCGAGAATGGGAAATCTTTCatttattcatgcaacagatatttaacgaagccctgccgtgttccaggcctgtgatagatgctggaacaggtacagaga----------tAc-------aggtgtcattaattgatcaggg--caacctctc---cttctgagt--cttgctggagcttcagatgc-ccctcacacagagctcgagggagcctc-aacaattgatcagaagtcaggcaccatggctcacgcatataatcccagcactttgggaggccaaggcaggtggatcactggagcccaggagttccagatcagctggggcaacatggcaaaaccccatctctattaaaaaaaaaaaaagtaactggatgtgatggtacacacctgtagtcccagctacttgggaggctgagaggtgggagaattgcttgagcccgggaagtcgggggtccagtgagccttgatcacaccactgcactccagcctgagtgacagagcaagaccctgacacacacacacacacacacacacacacacacacagattagagctgaaacaggagtagaaacctatctg-tatctctgATGA-GATCAGATC---------TTTCTGATGAACAGAAAGAATGTAACCCCTGTACTCACACCCTCTCTGCTGGTTACATATGTTAACACGATTTCTCAAATGAGGCTTTTGGTTGCAAATAAGAGAAAATCACTCACGCT-GGCCCTGTG--TTTTTCAAATTGTTTATTGTGATCAACATTTGAAAAAAGAGCCGAGACTCTCAAGAGTGCATTACCCACGGTAAGGGTGAATTTT-ACTTCTTGACACTTATTTCTCTTACATGTATCTATCTGTCTC-----AAATGAAAAATATATTTAGAAAGTTGAAAGCTATCCAAGTGAGTATAAGAAAAGAGTATCTCACCCTGAAGGCTAAGGACAGGGAGGGC---------------------------CACCAGGCCTCACGAGGACCCAGGAACCACAAAGAAGGCT-AGGAAGGAGCACAGGCGGTGACCATACTCTGGCTCAGTGGCTATGTGGGCTCTGGTCTCTCTCAGCTGTTCCATGCATATGAGGCCAAATGTGGCTACCCTAGAGCTTCTGAGCCCTCAACAGAGATGAACTGGACTCTCTGCAGCCCCACTCTAAATTCCTAAGAGAGAAGTTGATTGACCCAATCAGGGTCAGGAGAAGGAAGGGAGGAGGAAAGGGAGGAGAGAAGAGCCTCTTCGTCTCTTGCCTACCACTGGCCAGGCAATTGTAGCCAAGGGGGCTGGAGTGTAAATGCAAACATAGCCATCAAGGGTtgtgtatgtgtgtgtgtgtgtctgtgtgtgtgtatgtgtgtCTCTTGGGTAGGTTAGA-TCTCCCAGGAGGTCCCTACTAAACAGACTTAAGCCCGCAAAATTTTAGCTCTCCAGCCTCACACACTCCACCCCTCTACCATATTGAATCTTCCCAAACCAACTATGGCTTTCCCTAACTCCGGAGc------ttggcctggaatgccctgcttcccctctttcccctggggaacgcctgtccttcaggcctcagttcacacactgcctcccttgcaaagctctccTCCCATCCCCGGAGTCCCT--CTTCCCCTTTGTTCTTTGGGTTCTATGCTTCTTCCCTCATAACTCCCACCAGGTTGTGTTAAAATGAGTTGTTCAAGGTCCTGTCTGTTCCACTAGATTCTGAGCAACTTGGAGAACGAAGATCCAAACTTCGCTGCCTTTATTTCCTCCTTTGTTCTTTTCTCATCCCCAAGTCCCTTCCAACTTGGAGTTATgaagaaaggaaggaaggaagggtgggagggaagaaCAGGAGGGGATCCCACAGG-AGAATGTGTATAGGGAGAGGACTCAGACTAGCTAAAGCTTTTCCCTCATAATTAATAGCAAATACCATGTTACCTGAATTTAATTCACAGTAGCATACAAAAGACTCGCTTTGTTCT-------CCCCA---------TTGATGTCATCAGAGG--------------------GCTGTGGG--------------CAGGCCTAATCTTGGCTCAGGAGGCCCTCCAGCCTGGATCTAAAGAGCAGCAGATGggccaggctcggtggctcatgcctgtaatcccagcattttgggaggccgaggcgggtggatcacgaggtcaggagtttgagaccagcctggccaagatggtgaagcctcgtctctactaaaaatacaaaaattagccaggtgcggtggtgggcgcctgtatttccagctacccgggaggctgaggaggctgaggcaggagaatcgcttgaacccgggaggcggaggttgcagtgagccgaggtcacgccactgcactctagcctgggcaacagagcaagactccgtcaaaaaaaaaataaaaaaataaaaaaataaaaaaaataaaGAGGAGCACACATCTCTGCCCATCCTAACTCCCACTTTGACATTGAGGTCCCCAGGATGGAGGGTCTGCCTCCATCTGCCTTGTCCCCTG-CAATGGTGGGAAGGTGATGGAGCTCAAGTCTAGAGGCCACCAGCTTCTTAGGGAGG--TAGGAGGTG---------------GAGGGTGGGGTGC-GGGCCCTGCACACAACTGCCAAGTGAGGATGGGGGTGGG-GTCCACCTGAGGATAAGTAACAGTGAGGCTGGTGCAGAGGACCCAGGTGGAGGTAGACAGCAGAATTTGTGGTGGGGT--GGATGGCAC-ATTATATAAGCCTCTCTTGC------TGCCCTGT---TTACTGAGATTGTTTCAttatcttttttggcttttgtttttaagagatggggtcttgctgtgtcacacaggctggagtgcactgtgtgatcatacctcactgcagcctcgacatcctgggctcaggcaaacctcccaccttggcctcccaagtagctgggaccacaagcgtttgccaccacactcagctatttttatttttattttta--ttttttttagagatggggtcttgctgtgtcgcccaggctggtcttgaactcctgggctcaagcgatcctcctgccttggcctcccaaagccctgggattataggctgagccaccacacccagccACATTTCATCTGTGCAGCTCCAGGGGCTCCACATTCT-ACTCTTCTCATTTCTTCTCCAGGGTACCC----------ATGGCAAGGGATGAGGGT--AGAAGATGGGGCA--GCCAGGCCTTGATTAAAGGAGAAGGAAGGCAGCCTGTGGAGAGG---GCAGCC---C---AGGGAG---TGCAGAGAGAAGTGGGCCATGAGGGAGA---CAGCAGAGTGCAGGCTGCGTCC---CAAATGAGCATACAGCCCACTGTGAGCCCACC--ATCTTCCTAGA-GA--CCCCTCTCCTCTCC-AGGAGCTGCTTCAGTAGCACTCA---------GAGGAAAGAATGATGC--------TGTATCAACATTTCAGCAGCTCATCTTTTAACTCTAAGAAAATGGCAGCTCCTAAATGTTCAA--AACTGCTTTGGAAACTTCT---GGAGAGAGGTTTTGCAGCTCAGGCAGACAGCTGATCGCGGCCTTTCTTCCACCCCAACCCATGCTCTCCCCATGCT--CTCCTGCCACAGCTGCAGCGGGCCCCTGGGTCCTACATTTGCAG-CCCTTTGTCTCTGAGCT-----CAGACTTCCAATTCCAAGCGGCAGCTGGGCAGGCTCACCAGCATGT---CCAGCCAGTACTAGGACATCAGCAGGAGC----CCAACCACCTCTTTCCAAAATCTCTCCTCATGTCTCTCCTAGTTTCCATCTCCATCCTTCTAGTCAGCCAGGCTGAAAACATT-----------------TGCTCCTCAGGGTGCAGAAGGGAAAGCTTTGCCTCCCTTCCTGGTGCTCACTGCCCCTGCGATTCCAGCCCAAGCCCTCCCCGGCTCCTCACC----------CTGGTGTCAGCTGGAAGCCACCATCTCCTAAACCCACCTGtgttcttccacctctgc--------cagggctgc-cctctcctccaccttcacaaactcaattcctacccattctcaggtcccttatcaaatgccatctcctccatgatgcctccctgattccccTGCTGGAaataatggtgataacagctaag--gcattggggttggctacgtgccaggcaaggagttggcactttacatgctttatctcatttcagccacataacatcgacaggt-ggcattatgattcatatcatccccatctgatagccaggaaaactgagtcccagagaggttagc-cactttcctagggccCTGTGCTCTGACTCAAGCATAGCTCTGAGGAACTCTAGCATTCATCAGTTTAAGCACCATGACTTTCTTTGCTGAGTCACCCAAGGCAT-TTCTTCATTTAAATGTTCTTCCTTGGCCAGGCGCAGTGGCTCAggcccaatgcggtggctcacgcctgtaatctcaacactttgggaggccgaggtgggcagataatctgaggtcaggagttcaagaccagcctggccaacatggtgaaaccccatctctactaaaaatacaaaaaaatgaggctgggcgtgatgactcacacctgtaatcccagcactttgggaggccaaggcaggtggattacatgaggtcaggagttcgagaccagcctggccaacatggtgaaatcctatctctattaaaaatacaaaaaattagccaggcatggtggcaggcacctgtaatcccagctacttgggaggctgaggcaggaaaatggcttgaacccgggaggtggaggttgcagtgagccaaggttgcaccattgcactccagcctgggcaaaaagagggaaacatcgtctaaaaaagaaaaaaaaaaaattagccaggctgggtggtgcatgcccgtaattccagctactcaggaggatgaagcaagagaattgcttgaacccaggaggcagagattacagtgagctgagatcacaacactgcactccagcctaggtaaagaacaagactccatctcaaaaataaataaataaaaataaaTGTTCTTCCTTGCAATGAAGTTAAATATGTAAATTCTCAAACCAGTTGCTTAAGGGCACAGTTTTGTTCTTTACCTATATTTTTAACAAATATTTTATGTAAGTAGTTGAC-AAAATCAAATACTGT-GTACACTACCGAGGCTTCCCTGGGAAAGCCATCAG-CCTCTGCCCCATCCCTTCCCACTCCTGATT-CCACTTTCCTGTGTTTCCATATCTTTTTCATGTCTGTTTCTGGCCCACAGTGGGCGATCAATACATGTTAGCCACCAACCATCAAACCTATATTGAGTAATTATGGTATGTCAGGCACTATGCTCAATGAAATTGTAttaggcttgtacaaaagtaattgtggtttttaagagtaatggcaaaaacggcagttactttcgcaccaacTATTTGCTGCCTTGAATTATTCCTCCTCTC-CTCATCCCTAAACCCTGCTCCTCCCAGCCATTCTTCCTCCCCTTCTTGGGCCATGGCCAGGCCCCACCCAGGTACTAAGACTCAGGTGAACCAAGGAAGACTTAATGCCCACTCTTTTCTGATGCCCATGTT--GGCATGTGTTAAGtcggttagcattaagtttggctgcatttagcagagacccaaaagaacagtgccttttaaaaggcagaggttatgtctctcacacacacccagcacaagtccaag-------------------------accagcatggcatctcagctccatcaa--cctcaggaaccgagctcctgcagctccctgccctgcagttgataaggtgaggtctttgtcctcctggttcaagatggtgctagaatgttggctaccatatctatagtccaggcatcagaatggagcaagggatgaaaaaggaagagatgaaggcacacgacaggttcctgagagctggcacaggacacttctgcttatatttcactggccagaacttagtcacatggtcacacctagttgggagactctgagaagtaa----agtatttattctagatggccatatccctacc-taagacttggagttttctatgactggggaagaacggaagacaagatattgggaaagactagcagcctctactaAAAGGGTGATCtgtgttgatgtgcgtgtgtgtgtgatgtttgtatg---agcatgtgtgt-tatgtgttgt--gtgtTGGTGGGGCA--GATTCTTGCGAGCACTTTGGTCTCAGATGGACCTGCTACCAGTTCTCTCTGCAGACCCCCATAGGTTTCTCCTAAACCTGGCCT-CTCCTATTAGGCAGCCTTACTCAGCGGCAGCTTCTCAGCTCCATGTTTTCAAGGAACCACAATTTATTTCCAGCATCCACTGAAGCATATTATCAGTGGTGATAGAGGGGGCTTGTAAAACTGTTTTTCCACTTAGGTATTAGAGGGTGGCCATTATTTGAGAGTGAC-----TATGACCACAGTTAATCTGGTAATAAATTCTCTTGGGTAGGAGGGGGAAAGGAAAGGATGCTTTAAGGAAGCATCTTGCCAGGAGACACAAAGCTAACAAGAGTGGAGCCTGCAG----------------------------CTGGAGCCGCAGAGCCTAATCACTACACCCGCCCATCTCTGCTAGGGTTTCATGACTTCGTATCGGGGATTAGCAGTATTTAACTCTGTTGCACAAACATTTGGTGTA-----TTATTCAGGTAACAAGTAGCTAATAGAGGAAGTTTTACTTTTTTAAGACATAA--------------------ATTTGCCTTTTCCCAAATTACTTGGTACATAGTAC-TTTTCATGTTTGAAGTTGAGATGTGGGTACAATACCATAGCTTTATTCCAGAGCAGGGTATTTGTTTCCAAATGCCATGTTCCCAGCAGCTGCCCTTGACTGGGAATTGGGGTG-----TGATTTGGGCTTTTCCTTAAATCCTTGA-----GGAGCTGGA---GGGGTGGGTGGCTCGCACTCCTGCTTTctgg---------atctgaatc--------------ctgactctgtcatggacctgtt-tgactttgggcaagttgactcctattcctgagccccatat-ttttctcttctgtgaaattcagattaaaaA-AACATGGCTTTGATCAAACATTATAAATAATATATAGACAGACTGCTTGTTTTTATTGTATTGCCAG-AAATGAATCCTACTAATATTGCCATCTATGGACAGAAAATGTATTACCTGTCTTCATCAAGACCCAGACGAGGAAGAACACGAAAAGCGGAGATTAATTTTACTGCCATCTCCAGAACCGTCATCCTAATATTTACTTACAT-TTTATTATTATTTCAGGCTCATGCACATATACTTAGCATGGATCATTGGCCACAGACTCGCATACATTTAACTTTATTACCTTT-TGCCTCATGTATCTCATTAAAATTTTGCTGCTTAATCAAGGATCTGCATATTATTTTAATTTTAGAATTCACAGTTCCAAGACTTTGAAAGTTTCAAGCGTTCTGGGTGaatgtgttatgc--tctctcccgccaccatgtctttataccccctgatttctcagccact-atggcaaccactttctactcttagtagcccatatttag--tccaatccccagctcaggagacacttcttccaggg--agccccctgtgccttccagtagtatcttgtacctgccctttttgcaaagctctttcctcctggcttagaatggcccattgacctgtttgtttctcctattaaactgtaagccactcgagggtagagagcatctgttgttcaccattgcatcctcggtgctgagcactgcgtctgacatattatttagaaggtcagtaagtgctagtgggatTCAGGCTCCCAGTGGGTGGGAGAGAAAGGACGTAAGGAAGCAAGTGGTAAAGGCCCTCACAGA-GTATCAGCAGGCTGGTGTGA-GGGAGAAATGCAGAGGATGGGTGAGTAGCA-----TAATCGCTAATGAT-AGGGTAATGATAGAGCACATTTCACAACACCTTt-aagccctttcacgtgcatcagataatttgatcctcataaaagcctagagatagatatattacagg-gatgaaggtggagtattttgtggttatgtgatatg-tttaaaattatgcagtgagtaaatgactgggttcaaaccagaccttaaaagtctgttatctttccCTCG-AGCATGCAATGAAGTCTACATCATCCCTACCATGTCCATTTGATCACACCCTGGCCTCACAGCTCTGTGGTCTACAGGATACCTCATGGTGGTTTTATTGACCAGACAATAATCCTCTTTCTAAGGGGATGCATTTCATTAATACATATGTAGATCATGAATTGTCTTTGACTTTGAGGGGATGGTAGC----CAGAGCAGAAAGCAAAGCTGATTTTCATCCCCGTCTGGTAATGTGGTTGGTAATGTGAAGA-TGGGTGTATTCTGAGATACCGGCTCCTTGCAGTGTGTGGTTCCTTCTGTTTTCAGGCCC------AAGAAGCCCATCCTGGGAAGGAAAATGCATTGGGGAACCCTGTGCGGATTCTTGTGGCTTTGGCCCTATCTTTTCTATGTCCAAGCTGTGCCCATCCAAAAAGTCCAAGATGACACCAAAACCCTCATCAAGACAATTGTCACCAGGATCAATGACATTTCACACACGGTAAGGAG---AGTATGCGGGGACAAA---GTAGAACTGCAGCCAGCCCAGCACTGGCTCCTAGTGGCACT-GGACC-CAGATAGtccaagaaacatttattgaacgcctcctgaatgccaggcacctactggaagctgagaaGGATTTGAAAGCACAGGGC-TCCACTCTTTCTGGTTGTTTCTTTTGGCCCCTCTGCCTGCTGAGATTCCAGGGGTTAGTGG--------------------------------------------------------TTCTAATTCTAAACCACTCCAAGAACATTTGATTTTGCTACATGTTTCCATTTAAAAATCATAGGATTTGggctgggtgtggtggcttgtacctgtcatcccagcactttgggaggccaaagcaggaggatcattcgagcccaagagttcgagaccagcctgggcagcatagggagaccccatctctacaaaaataataaaaaatgttagctgggcatggtggtgtgtacctgtggtcccagctaggggaggctgagatggaaggatcacctgagcctgggaggttgaggctgcagtgggccctgatcatgccaccgtgctccagcctgggtgacagagtgagaccttgtctcaaaataaataaataaataaataaaAGTCATAGGATTTgatcaggcatgatgggtcacatctgtaagcccattgctttaggaggccaaggtaggaggatcagttgaggccaggagttcaagaccagcctgggcaacatggcaagacctctctctctaatttttaaaaaaataaaaaTTAAAGATAAGAAAAAAATCATAGGATTCTCATGAGGCCTCACGTGCTTATTTTCAACCTACCAAGGGGAAACCCAGGCCTCAGCGATTAGCTGAGC----------CACATGCAGGCACAG------------------------CCACTG-----TCTCTTTCCTTCCTGTCCCCTCTGTCCCCACCTTCTGCGCTCGCCTTCCTCCCTGACTTCACTTCCTTGAATCTTAGTGCCTACGACCAGAGGGAGCTGTGAAGTTCCTTG----TGTCCCATTGGCAGGAA-CAAGACCCCCAGAAGCATCTCCTCAGGGC------CTCTA-----TCCCATCTC-TAGATGTGCTTGTCATTAGG-Gttct-------------tgtagttccagctgatctctggccctgccgctcaaagatacccaaaagagcgagtctaccctttttcacattcaaccctctactgatttgcaaatagcagtcagtgcccaccctggtcttttctctggggtccagcaggcctagaccttcagccattttcctgatgaGGTCTGTAtttgaaattaggaagattaagtttgaatcttcacacttctgat----gtctgtgagatcttcagcaagttccttact--gtctttaagccttgt-tttcatcatctggataatggggatatcacacacta-ttcacaaggttgttatgaggcctaaattagctaaagcaATTGAATCCTCCTTACCCCCTGCATGGAGCTCTCTGGAGACTTCCACGTCTCCTGGTCATTGTGGGTGTCTTATGGTA-GTCTTGGGCAGTTAGGGAGAAGTTAGGTGTCTGGAAGCAAAGATGGCTCAGAACTAGATAGAGTC-TTGGGCATTTTATA-GATAAAAACTCTT--GTCTCCtttaaaaataataaaaaaaaattaGCTGGGCATATTAGCCACTCAGCAAGACTGCACGTGATAGATCCCGAGTGCCCCACCTTGGGTGGTGTAATACACAATATCACGGGAGCCCCGGGTAGTAACCACGGAGGTGTCAGCCTCAGTGCTGTGGGCAGATG-GATGGGGAGAGCC--TCCCGG-AACTGGAGTCACTGGAGCA----------------------------GGGTTGGGGGGCCTCACTGAGGGTACGGCCTTGATCTCTAAGGAGGAGGGACTGCCTGGAAAAGC-TGACTGGGAGGGAGGACTCGGCTGGGGGTAGAAGGGA----------CTAGGGAAGGCTGGGGGTGGGGGTGCTTATGGAGGACCTCAGATGCCTGGGGAACAGACTCCACTAAATAAAACATATGAAACCATGGCTGGTTCTTCAGCAGAGGCCATGTAGAGAAAGGAATGACCTAGGAAAGTTGGCCTGGAAGTGGAGGGAAGGATGGTGTGGGAAAAGCAGGAA--------TCTCGGAGACCAGCTTAGAGGCTTGGCAGTCACCTGGGTGCAGG-ATACAAGGGCCTGAGCCAAAGTGGTGAGGGAGGGTGGAAGGAGGCAGCCCAGAGAATGACCCTCCATGCCCACGGGGAAGGCAGAGGGCTCT-GAGAGCGA--TTCCTCCCACATG-CT-GAGCACTTGTTCTCCCTCTTCCTCCTGCATAGCAGTCAGTCTCCTCCAAACAGAAAGTCACCGGTTTGGACTTCATTCCTGGGCTCCACCCCATCCTGACCTTATCCAAGATGGACCAGACACTGGCAGTCTACCAACAGATCCTCACCAGTATGCCTTCCAGAAACGTGATCCAAATATCCAACGACCTGGAGAACCTCCGGGATCTTCTTCACGTGCTGGCCTTCTCTAAGAGCTGCCACTTGCCCTGGGCCAGTGGCCTGGAGACCTTGGACAGCCTGGGGGGTGTCCTGGAAGCTTCAGGCTACTCCACAGAGGTGGTGGCCCTGAGCAGGCTGCAGGGGTCTCTGCAGGACATGCTGTGGCAGCTGGACCTCAGCCCTGGGTGCTGAGGCCTTGAAGGTCACTCTTCCTGCAAGGACTACGTTAAGGGAAGGAACTCTGGCTTCCAGGTATCTCCAGGATTGAAGAGCATTGCATGGACACCCCTTATCCAGGACTCTGTCAATT--TCCCTGACTCCTCTAAGCCACTCTTCCAAAGGCATAAGACCCTAAGCCTCCTTTTGCTTGAAACCAAAGATATATACACAGGATCCTATTCTCACCAGGAAGGGGG-TCCACCC-AGCAAAGAGTGGGCTGCATCTGGGATTCCCACCAAGGTCTTCAGCCATCA---ACAAGAGTTGTCTTGTCCCCTCT-TGACCCATCT-----------------CCCCCTCACTGAATGCCTCAATGTGACCAGGGGTGATTTCAGAGAGGGCAGAGGGGTAGGCAGAGCCTTTGGATGACCA--GAACAAGGTTCCCTCTGAGAATTCCAAGGAGTTCCATGAAGACCACATCCACACACG--CAGGAACTCCC--AGCAACACAAGCTGGAA---GCACATGTTTATTTATTCTGCATTTTATTCTGGATGGATTTGAAGCAAAGCACCAGCTTCTCCAGGCTCTTTGGGGTCAGCCAGGGCCAGGGGTCTCCCTGGAGTGCAGTTTCCAATCCCATAGATGGGTC-TGGCTGAGCTGAACCCA---TTTTGAGTGACT----CGAGGGTTGGG-TTCATCTGAGCAAGAGCTGGCAAAGGTGGCTCTCCAGTTAGTTCTCTCGTAACTGGTTTCATTTCTACTGTGACTGATGTTACATCACAGTGTTTGCAATGGTGTTGCCCTGAGTGGATCTCCAAGGACCAGGTTATTTTAAAA---AGATTTGTTTTGTCAAGTGTCATATGTAGGTGTCTGCACCCAGGGGTGGG-GAATGTTTGGGCAGAAGGGAGAAGGATCTAGAATGTGTTTTCTGAATAACATTTGTGTGGTGGGTTCTTTGGAAGGAGTGAGA-TCATTTTCTTATCTTCTGCAATTGCTTAGGATGTTTTTCATGAAAA------------TAGCTCTTTCAG-GGGGGTTGTGAGGCCTGGCCAGGCACCCCCTGGAGAGAAGTTTCTGGCCCTGGCTGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAAAGGGCTGAAAGCCATTTGTTGGGGCAGTGGTAAGCTCTGGCTTTCTCCGACTGCTAGGGAGTGGTCTTTCCTATCATGGAGTGACGGTCCCACACTGGTGACTGCGATCTTCAGAGCAGGGGTCCTTGGTGT-GACCCTCTGAATGGTCCAGGGTTGATCACACTCTGGGTTTATTACATGGCAG-----TGTTCCTATTTGGGGCTTGCATGCCAAATTGTAGTTCTTGTCTGATTGGCTCACCC-AAGCAAGGCCAAAATTACCAAAAATCTTGGGGGG--TTTTTACTC-CAGTGGTGAAGAAAACTCCTTTAGCAGG-TGGTCCTGAGACCT-GACAAGCACTGCTAGGCGAGTGCCAGGACTCCCCAGGCCAGGCCACCAGGATGGCCCTTCCCACTGGAGGTCACATTCAGGAAGATGAAAGAGGAGGTTTGGGGTCTGCCACCATCCTGCTGCTGTGTTTTTGCTATCACACAGTGGGTGGTGGATCTGTCCAAGGAAACTTGAATCAAAGCAGTTAAC-TTTAAGactgagcacctgcttcatgctcagccctgactggtgctataggctggagaagctcacccaataaacattaagatt-gaggcctgccctcagggatcttgcattcccagtggTCAAACC-GCACTCACCCATGTGCCAAGGTGGGGTA-TTTACCACAGCAG--CTGAACAGCCAAATGCATGGTGCAGTTGACAGCAGGTGGGAAATGGTATGAGCTGAGGGGGGCCGTGCCCAGGGGCCCACAGG-GAACCCTGCTTGCACTTTGTAACATGTTTA-----CTTTTCagggcatcttagctt---ctatta-----tagccacatccctttga---aacaagataactgagaatttaaaaataagaa-----aata--TGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAAATGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCATGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCA
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/2.fasta Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,11 @@ +>Sequence 561 BP; 135 A; 106 C; 98 G; 222 T; 0 other; +gttcgatgcc taaaatacct tcttttgtcc ctacacagac cacagttttc ctaatggctt +tacaccgact agaaattctt gtgcaagcac taattgaaag cggttggcct agagtgttac +cggtttgtat agctgagcgc gtctcttgcc ctgatcaaag gttcattttc tctactttgg +aagacgttgt ggaagaatac aacaagtacg agtctctccc ccctggtttg ctgattactg +gatacagttg taataccctt cgcaacaccg cgtaactatc tatatgaatt attttccctt +tattatatgt agtaggttcg tctttaatct tcctttagca agtcttttac tgttttcgac +ctcaatgttc atgttcttag gttgttttgg ataatatgcg gtcagtttaa tcttcgttgt +ttcttcttaa aatatttatt catggtttaa tttttggttt gtacttgttc aggggccagt +tcattattta ctctgtttgt atacagcagt tcttttattt ttagtatgat tttaatttaa +aacaattcta atggtcaaaa a \ No newline at end of file
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/emboss_needle_out.fasta Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,676 @@ +>Sequence 561 BP; 135 A; 106 C; 98 G; 222 T; 0 other; +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +--------------------------------------------------------gtt- +-c----------gatgccta-aaataccttcttttg-----tcccta-------cacaga +-------------------------ccac------------------------------- +-------------------agt-------------------------------------- +--tttc-c--------------------------taatgg-ct-----ttacaccgacta +gaaattct-----------------------------------------------tgtgc +aa-gcac---------------------taattga--aagc------------------- +-----------ggt--tgg-------------c-ctagagtgttac--------cggttt +gtat---------------------------agc--------------tgagc-----gc +-----------------gtctcttgcc--ctgatcaaaggtt-cattttctctactttgg +aagacgttgt--------ggaa-----gaatacaacaagtacgagtc-----tctcc--- +-cc--------cctgg--tttgctgat----------------tactgg-----atac-- +-agttgtaatac-----ccttc-----------gcaac---------------------- +-ac----------cgcgtaact------atctatatg----------------------a +att------attttccct-------------ttatt--------atatgt---------- +----agtaggt-------------tcgtctttaatctt---------------------- +-cc----------------------ttta---gcaag-----------------tctttt +--------------------------------------------------------actg +tt----ttc--------------------------gacc--------tcaatgttca--- +------tgttc-----tt-----------------------------aggttg------t +ttt---------------------------ggataat--atgcggt-------------- +------cagttt----aatcttcgttgtt-tc----ttc------tta---------aaa +t----attt------att----catg-----------------gtttaatttt------t +ggtt-----------------------------tgtact----tgt---------tcagg +ggcc----------agttc---------------------------attat-----ttac +tctgtttgtat--ac----agcagtt-----------ctttt---atttttagtatg--- +---at---tttaat---ttaaaacaatt-----------cta---atg---gtca----- +-----------aaaa--------------------------------------------- +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +------------------------------------------------------------ +--------- +>hg17 +gtttgccatcttttgctgctctagggaatccagcagctgtcaccatgtaaacaagcccag +gctagaccaGTTACCCTCATCATCTTAGCTGATAGCCAGCCAGCCACCACAGGCAtgagt +caggccatattgctggacccacagaattatgagctaaataaatagtcttgggttaagcca +ctaagttttaggcatagtgtgttatgtaTCTCACAAACATATAAGACTGTGTGTTTGTTG +ACTGGAGGAAGAGATG-CTATAAAGACCACCTTTTAAAACTTCCCAAATACTGCCACTGA +TGTCCTGATGGAGGTATGAAAACATCCACTAAAATTTGTGGTTTATTCATTTTTCATTAT +TTTGTTTAAGGAGGTCTATAGTGGAAGAGGGAGATATTTGGggaaattttgtatagacta +gctttcacgatgttagggaattattattgtgtgataatggtcttgcagttacac-----a +gaaattcttccttattttttgggaagcaccaaagtagggataaaatgtcatgatgtgtgc +aatacactttaaaatgtttttgccaaaataattaatgaagcaaatatggaaaataataat +tattaaatctaggtgatgggtatattgtagttcactatagtattgcacacttttctgtat +gtttaaatttttcatttaaaaaaaaactttgagctagacaccaggctatgagctaggagc +atagcaatgaccaaatagactcctaccaac---tcaaagaatgcacattctC-----TGG +GAAAC-ATGTTTCCATTAGGAAGCCTCGAATGCAA----T--GTGACTGTGGTCTCCAGG +ACCTGTGTGATCCTGGCTTTTCCTGTTCCCTCCGCATCATCACTGCAGGTGTGTTTTCCC +AAGTTTTAA-ACATTTACCTTCCCAGTGGCCTTGCGTCTAGAGGAATCCCTGTATAGTGG +TACATGAATATAACACATAACAAAAATCATCTCTATGGTGTGTGTTGTTCCTGGGGTTCA +attcagcaaattttccctgggcacccatgtgttcttggcactggaaaagtaccgggactg +aaacagttgatggcccaatccctgtcctcttaaaacctaagggaggagaTGGAAAGGGGC +ACCCAACCCAGACTGAGAGACAGGAATTAGCTGCAAGGGGAACTAGGAAAAGCTTCTTTA +AGGATGGAGAGGCCCTAGTGGAATGGGGAGATTCTTCCGGGAGAAGCGATGGATGCACAG +TTGGGCATCCCCACAGACGGACTGGAAAGAAAAAAGGCCTGGAGGAATCAATGTGCAATG +TATGTGTGTTCCCTGGTTcaagggctgggaactttctctaaagggccaggtagaaaacat +tttaggctttctaagccaaggcaaaattgaggatattacatg-ggtacttatacaacaag +aataaacaatttacacaatttt--ttgttgacagaattcaaaactttatagacacagaaa +tgcaaatttcctgtaattttcccatgagaactattcttcttttgttttgttttgcgacAG +GGTTGCGCtgatcctcccgcctcagtctccctaagtgctgagatgttgcaggaagtcagg +gaccccgaacagagagatcggctggagccgtggcagaggaacataaattttgaagatttc +a-ttttaatatggacacttatcagttcccaaataatacttttataattttt--tatgcct +gtctttgctttaatctcttaatcctgttatcttcataagctaaggatgtacgtcacctca +ggaccactgtgataattgtgttaactgtacagattgattgcaaaacatgtgtgtttgaac +aatatgaaatcagtgcaccttgaaaaagagcagaataacagcaatttttagggaacaagg +gaagacaactataaggtctgactgcctgcggggtcgggcaaagggagccatatttttctt +cttgcagagagcctataaatagacctgcaagtaggagagatattgctaatttcttttgct +agcatggaatattaatattaacaccctgggaaaggaatgcattcctggggggaggtctat +aaatggccgctctgggaatgtctatcctacgcaatggagataaggactgagatacgccct +ggtctcctgcagtaccctcaggcttactagggtggtgaaaaactccgccctggtaaattt +gtggtcagaccagttttctgctctcgaacactgttttctgttgtttaagatgtttatcaa +gacaatacgtgcaccgctgaacacagacccttatcagtagttctcctttttgccctttga +agcatgtgatctactccctgttttacaccccctcaccttttgaaacccttaataaaaaac +ttgctggtttgaggctcaggtgggcatcacagtactaccgatatgtgatgtcacccccgg +cggcccagctgtaaaattcctctctttgtactctctctctttatttctcagccagctgac +acttatggaaaatagaaagaacctacgttgaaatattgggggcaggttcccccaataTCT +GGTGCCCAACGTGGGAtactgagattacaagcatgagccactgcatctggcctcttcttt +tgatttttttttttcaaacttttacaaatgtagaaaccattcttagcttttgggcattac +caaacccggcagtggcaggctcggttcaccaacgtcatttgcagttccccgCTTTATGTT +ATGGgttttgttttgttttgttttttttattgagacagagtttcactcttgttgcccagg +ctgtagtgcaatggtctgatcttggctcactgcaacctccacttcccaggttcaagccat +tctcctgcctcagcctctcaagtagctgggattacagacactcaccaccacacctggcta +attttgtatttttagtagagatgaggtttcaccatgttggccaggctggtctcgaaatcc +tgacctcaggtgatccacccaccttggcctcccaaagtgctgggattacaggcttgagct +accacgcctggctGGGTTGGTTCTCAATGGAGTGGTTTGTTTTTGGAGCTGCTCTGCGCA +Gtggggaccagaataggcctgggttcctagcccattgctattccttaccagctgtggatt +ctaaggaaagtcatttaacctcgctggaccttagattcctcatccctgaaGCCCAAGGGT +aaaacaaaacaaaacaaaacaaaacaaaccaaCCCATCATGTAAAGCGGGGAACTACAAA +CGATACAGGTGAAACATGCCTACCACACCACTCACAGGCTATGATGACAAAAACGTGGCT +ACATCTGGGACCACCCCCCAACCCCCACTTTGTACGTAGGAAATACGGAGTTGAGGATGG +AGACCCACAGTATGTCCAGAGTGTCCCCAAAGGCCACAGTGCCCGCCTGGAGCCCTCCAG +AGAGCGTGCACTCCCTGGGGTGCCAGCCAGAGACAACTTGCCCTGAGGCTTGGAACTCGA +TTCTCCGCGTGCCAGAGAAGGGGTGGGACTTCAGAACCCCCAACCCCGCAATCTGGGTCG +GGGAGCCTGGCGCACTGCGGGCCGCTCCCTCTAACCCTGGGCTTCCCTGGCGTCCAGGGC +CGTCGGGGCCGAGTCCCGATTCGCTCCCACCCCGAAGCCGCGCCAGGACCAACGAGGGCG +CAGCCGTATGCCCCAGCCCGCTCCGCGGAGCCCCTCACAGCCAcccccgccccgaccgcg +ccccgcgcggcTCGAAGCACCTTCCCAAGGGGCTGGTCCTTGCGCCATAGTCGCGCCGGA +GCCTCTGGAGGGACATCAAGGATTTCTCGCTCCTACCAGCCACCCCCAAATTTTTGGGAG +GTACCCAAGGGTGCGCGCGTGGCTCCTGGCGCGCCGAGGCCCTCCCTCGAGGCCCCGCGA +GGTGCACACTGCGGGCCCAGGGCTAGCAGCCGCCCGGCACGTCGCTACCCTGAGGGGCGG +GGCGGGAGCTGGCGCTAGAAATGCGCCGGGGCCTGCGGGGCAGTTGCGCAAGTTGTGATC +GGGCCGCTATAAGAGGGGCGGGCAGGCATGGAGCCCCGTAGGAATCGCAGCGCCAGCGGT +TGCAAGGTAAGGCCCCGGCGCGCTCCTTCCTCCTTCTCTGCTGGTCTTTCTTGGCAGGCC +ACAGGGCCCCACACAACTCTGGATCCCGGGGAAACTGAGTCAGGAGGGATGCAGGGCGGA +TGGCTTAGTTCTGGACTATGATAGCTTTGTACCGAGTTCTAGCCAGATAGAAGGTTACCG +GGAGCTGGGGAGCGTTGGATTTGCTGCTGGGCTGTGCCGGTGCCCAGAAGGCAGGACCTT +GCAGAACCAGCCAGGTCCCTGGGAGACTGTCAGACCCACCAACCTGGTGGCATTCGCAGA +GCTGAGATGCATTGGAAATTGCCTTGGGCACATCCCCAAAGATCAGGATGTCCCACCCCA +GTCTGAAGGAGATAAAGTTGGGGGTAGGAGAGACGCAGATGCAAGTGATCAGTCTCAGTC +CCAGACATTGCCTTGCTCTGCGGGTAGGAATTCAGGATTCATTTTCCAGGGAAGTTCCTG +ACCTCTGAATGAGAGGGGCTGTGTAAGGCCAATGCCTGGGAGGAAGGCAAGGATGAGTAG +AGGTGGGGGGAAACAAGTGTCAGGAAGACTCAAAATCTTCCAGAGAAATTGTGCAGGGTC +TTACCAGATCTGTCCTCAAAGCCATGCAAATTGCCTTCTTTGCAATGCATACAATGAGGT +GTCTCTGGGGGTCAGAACTGGTTATTAGGGAACTTCTAGCCAGGACTGCTAAATACGCGC +TGTTGGCCCACCAGGCTCACCTATAGCCTTCCTTCAGTCTGGGCTTGGTTTGGATTTCAC +TGTGGGTGCCATCGCCTTTACACTCCTGTTTCTATAGTTTAAAGATAGTGGTGCTTTGGG +AAAGTGACTCCTTAAATACAGTTAGGTCCAAGTGAGACAAGTGGCCTGGCTGTCATTTCA +GAATAGCAGCTTCCAAGAGGTGATTAATTTCTGTTGGAAGGGTGATCTTTGGGGAGGTGG +GTGAAGAGCAGAGACTTGGTGGTACCGTTCCAGGAGCACAGGCTCTCTTCCTTTGCAGTG +CAGAATGACCTCTGGCAGCCGGAGTTGTGTTTGTTCTGTAGGATTCTGAGGTGGGCCATG +GGCAGCTGGAACTGGGGAATTTTGCCAATCTCTTTCATATTAGGATTGTCTGCAGAACCA +GATATGGAGGCTTCTAGCAACGTGAGTGCTCCTGTTCTAATGCCCTTAGAAACAAGAAGG +CCACACTGATCATTTCTCTCACTTAGGCAGGGAGACAAGGCAAGAGAGAAACAGTGGATG +CTTTTAGGTTCTTTCCCTTCCCAAGCAGTTGTGGACATTGGGCTGAGGGGAACATTTCCA +CATTGGCTAAAGGAGCGTCCTCCTCATATTTTGTACATTTTATACCCAAAATAACTCTTC +TTGGTATTTGGGGAAATATTTTCCTCCCCGTCCATTCCAGGAAATGGCTCCAAGTGCCAA +GGACAGAGCCAGGGAAGTTGCAATGAATTCCTGCCCGTCAGCCCCAGGCAGATGCCTTGC +ACGTCTGAGTGGCCCATGCAGAGCGTGGAGGTGGCCGCCACGGAACCTGGGTCAATGTCC +CACCCCCGCTTAGATGCCACCAGGGGCGTGGGAGCCAAGGAGAGAAGAGGGGCTCCAGGA +AGGTAGAGTCCTTGTGTCTTGTGCATCTGTGAACAGCACTGGTATGATTTAAAGGAAAAT +TGAGCCAAATTTTCCGGCAGTCAGTTACCCCATCCCCACCGGGGTAGGAGTCTGGCAGCC +GCAGCTCCATTCTGGCCAGTCGGCAGAGAGCCTTGAAATTCTTCTTTGTCCACACAGTTG +TCTCAGAGAAACAGAGAGGTTGTTTCTGCTTAAAAACAACACACTTGGTGTCTGGGCCCA +CAGACTCCTTTGCACTTATTCCACGTGTGACAGCCAATGTGCCTCGTTGCTTAGCAGACA +GCATGTTACCGTCTTTCCTGCTCAGTTTGTTAGCTCTATGGAATGGAATTTATAATCAAT +GCCCATACCAACATTTCACTAATATCATAGGAGATTTAGTCTCCATCTGGGTGTACATTA +CATTTGCTCTGGGGTGCTCCAGGCTGGGGGGTTGCCAAGGAAGAGAAGAGAAACCGCAGA +GAAGACGGGAGGGCAGGGCAGGGGTCTCTGAGAAGGGGAGGGGTCCCAGAGTGCAGGAGC +AGGAGCCAGGCTCATGAAAGGGGCCACGGGCGGGAGTATCCAGGGACGGCAGTCAAGATG +GAGCACAGCTTAGGAAGCTGAAGGGAATCCTGGCCCACCTGGGTGCTAGAGGGCACATAG +GAAGTGCAGGAAGCAGACCAAGGTCCCCAAGAGAGGGAGACCTGGACGCTGAAGCATTTT +CTGTCTTTATTAAGACAACTCCGTAAGAATTCCTGCTGGGCCAAAGTGAATTCTAGGATG +CGACTTTAAGATGGGAGCAAGCGAACCATTGAGGAGGCAGGTTACCCTAGTTAGCCAATG +CAGATCGAGAATGGGAAATCTTTCatttattcatgcaacagatatttaacgaagccctgc +cgtgttccaggcctgtgatagatgctggaacaggtacagagatAcaggtgtcattaattg +atcagggcaacctctccttctgagtcttgctggagcttcagatgcccctcacacagagct +cgagggagcctcaacaattgatcagaagtcaggcaccatggctcacgcatataatcccag +cactttgggaggccaaggcaggtggatcactggagcccaggagttccagatcagctgggg +caacatggcaaaaccccatctctattaaaaaaaaaaaaagtaactggatgtgatggtaca +cacctgtagtcccagctacttgggaggctgagaggtgggagaattgcttgagcccgggaa +gtcgggggtccagtgagccttgatcacaccactgcactccagcctgagtgacagagcaag +accctgacacacacacacacacacacacacacacacacacagattagagctgaaacagga +gtagaaacctatctgtatctctgATGAGATCAGATCTTTCTGATGAACAGAAAGAATGTA +ACCCCTGTACTCACACCCTCTCTGCTGGTTACATATGTTAACACGATTTCTCAAATGAGG +CTTTTGGTTGCAAATAAGAGAAAATCACTCACGCTGGCCCTGTGTTTTTCAAATTGTTTA +TTGTGATCAACATTTGAAAAAAGAGCCGAGACTCTCAAGAGTGCATTACCCACGGTAAGG +GTGAATTTTACTTCTTGACACTTATTTCTCTTACATGTATCTATCTGTCTCAAATGAAAA +ATATATTTAGAAAGTTGAAAGCTATCCAAGTGAGTATAAGAAAAGAGTATCTCACCCTGA +AGGCTAAGGACAGGGAGGGCCACCAGGCCTCACGAGGACCCAGGAACCACAAAGAAGGCT +AGGAAGGAGCACAGGCGGTGACCATACTCTGGCTCAGTGGCTATGTGGGCTCTGGTCTCT +CTCAGCTGTTCCATGCATATGAGGCCAAATGTGGCTACCCTAGAGCTTCTGAGCCCTCAA +CAGAGATGAACTGGACTCTCTGCAGCCCCACTCTAAATTCCTAAGAGAGAAGTTGATTGA +CCCAATCAGGGTCAGGAGAAGGAAGGGAGGAGGAAAGGGAGGAGAGAAGAGCCTCTTCGT +CTCTTGCCTACCACTGGCCAGGCAATTGTAGCCAAGGGGGCTGGAGTGTAAATGCAAACA +TAGCCATCAAGGGTtgtgtatgtgtgtgtgtgtgtctgtgtgtgtgtatgtgtgtCTCTT +GGGTAGGTTAGATCTCCCAGGAGGTCCCTACTAAACAGACTTAAGCCCGCAAAATTTTAG +CTCTCCAGCCTCACACACTCCACCCCTCTACCATATTGAATCTTCCCAAACCAACTATGG +CTTTCCCTAACTCCGGAGcttggcctggaatgccctgcttcccctctttcccctggggaa +cgcctgtccttcaggcctcagttcacacactgcctcccttgcaaagctctccTCCCATCC +CCGGAGTCCCTCTTCCCCTTTGTTCTTTGGGTTCTATGCTTCTTCCCTCATAACTCCCAC +CAGGTTGTGTTAAAATGAGTTGTTCAAGGTCCTGTCTGTTCCACTAGATTCTGAGCAACT +TGGAGAACGAAGATCCAAACTTCGCTGCCTTTATTTCCTCCTTTGTTCTTTTCTCATCCC +CAAGTCCCTTCCAACTTGGAGTTATgaagaaaggaaggaaggaagggtgggagggaagaa +CAGGAGGGGATCCCACAGGAGAATGTGTATAGGGAGAGGACTCAGACTAGCTAAAGCTTT +TCCCTCATAATTAATAGCAAATACCATGTTACCTGAATTTAATTCACAGTAGCATACAAA +AGACTCGCTTTGTTCTCCCCATTGATGTCATCAGAGGGCTGTGGGCAGGCCTAATCTTGG +CTCAGGAGGCCCTCCAGCCTGGATCTAAAGAGCAGCAGATGggccaggctcggtggctca +tgcctgtaatcccagcattttgggaggccgaggcgggtggatcacgaggtcaggagtttg +agaccagcctggccaagatggtgaagcctcgtctctactaaaaatacaaaaattagccag +gtgcggtggtgggcgcctgtatttccagctacccgggaggctgaggaggctgaggcagga +gaatcgcttgaacccgggaggcggaggttgcagtgagccgaggtcacgccactgcactct +agcctgggcaacagagcaagactccgtcaaaaaaaaaataaaaaaataaaaaaataaaaa +aaataaaGAGGAGCACACATCTCTGCCCATCCTAACTCCCACTTTGACATTGAGGTCCCC +AGGATGGAGGGTCTGCCTCCATCTGCCTTGTCCCCTGCAATGGTGGGAAGGTGATGGAGC +TCAAGTCTAGAGGCCACCAGCTTCTTAGGGAGGTAGGAGGTGGAGGGTGGGGTGCGGGCC +CTGCACACAACTGCCAAGTGAGGATGGGGGTGGGGTCCACCTGAGGATAAGTAACAGTGA +GGCTGGTGCAGAGGACCCAGGTGGAGGTAGACAGCAGAATTTGTGGTGGGGTGGATGGCA +CATTATATAAGCCTCTCTTGCTGCCCTGTTTACTGAGATTGTTTCAttatcttttttggc +ttttgtttttaagagatggggtcttgctgtgtcacacaggctggagtgcactgtgtgatc +atacctcactgcagcctcgacatcctgggctcaggcaaacctcccaccttggcctcccaa +gtagctgggaccacaagcgtttgccaccacactcagctatttttatttttatttttattt +tttttagagatggggtcttgctgtgtcgcccaggctggtcttgaactcctgggctcaagc +gatcctcctgccttggcctcccaaagccctgggattataggctgagccaccacacccagc +cACATTTCATCTGTGCAGCTCCAGGGGCTCCACATTCTACTCTTCTCATTTCTTCTCCAG +GGTACCCATGGCAAGGGATGAGGGTAGAAGATGGGGCAGCCAGGCCTTGATTAAAGGAGA +AGGAAGGCAGCCTGTGGAGAGGGCAGCCCAGGGAGTGCAGAGAGAAGTGGGCCATGAGGG +AGACAGCAGAGTGCAGGCTGCGTCCCAAATGAGCATACAGCCCACTGTGAGCCCACCATC +TTCCTAGAGACCCCTCTCCTCTCCAGGAGCTGCTTCAGTAGCACTCAGAGGAAAGAATGA +TGCTGTATCAACATTTCAGCAGCTCATCTTTTAACTCTAAGAAAATGGCAGCTCCTAAAT +GTTCAAAACTGCTTTGGAAACTTCTGGAGAGAGGTTTTGCAGCTCAGGCAGACAGCTGAT +CGCGGCCTTTCTTCCACCCCAACCCATGCTCTCCCCATGCTCTCCTGCCACAGCTGCAGC +GGGCCCCTGGGTCCTACATTTGCAGCCCTTTGTCTCTGAGCTCAGACTTCCAATTCCAAG +CGGCAGCTGGGCAGGCTCACCAGCATGTCCAGCCAGTACTAGGACATCAGCAGGAGCCCA +ACCACCTCTTTCCAAAATCTCTCCTCATGTCTCTCCTAGTTTCCATCTCCATCCTTCTAG +TCAGCCAGGCTGAAAACATTTGCTCCTCAGGGTGCAGAAGGGAAAGCTTTGCCTCCCTTC +CTGGTGCTCACTGCCCCTGCGATTCCAGCCCAAGCCCTCCCCGGCTCCTCACCCTGGTGT +CAGCTGGAAGCCACCATCTCCTAAACCCACCTGtgttcttccacctctgccagggctgcc +ctctcctccaccttcacaaactcaattcctacccattctcaggtcccttatcaaatgcca +tctcctccatgatgcctccctgattccccTGCTGGAaataatggtgataacagctaaggc +attggggttggctacgtgccaggcaaggagttggcactttacatgctttatctcatttca +gccacataacatcgacaggtggcattatgattcatatcatccccatctgatagccaggaa +aactgagtcccagagaggttagccactttcctagggccCTGTGCTCTGACTCAAGCATAG +CTCTGAGGAACTCTAGCATTCATCAGTTTAAGCACCATGACTTTCTTTGCTGAGTCACCC +AAGGCATTTCTTCATTTAAATGTTCTTCCTTGGCCAGGCGCAGTGGCTCAggcccaatgc +ggtggctcacgcctgtaatctcaacactttgggaggccgaggtgggcagataatctgagg +tcaggagttcaagaccagcctggccaacatggtgaaaccccatctctactaaaaatacaa +aaaaatgaggctgggcgtgatgactcacacctgtaatcccagcactttgggaggccaagg +caggtggattacatgaggtcaggagttcgagaccagcctggccaacatggtgaaatccta +tctctattaaaaatacaaaaaattagccaggcatggtggcaggcacctgtaatcccagct +acttgggaggctgaggcaggaaaatggcttgaacccgggaggtggaggttgcagtgagcc +aaggttgcaccattgcactccagcctgggcaaaaagagggaaacatcgtctaaaaaagaa +aaaaaaaaaattagccaggctgggtggtgcatgcccgtaattccagctactcaggaggat +gaagcaagagaattgcttgaacccaggaggcagagattacagtgagctgagatcacaaca +ctgcactccagcctaggtaaagaacaagactccatctcaaaaataaataaataaaaataa +aTGTTCTTCCTTGCAATGAAGTTAAATATGTAAATTCTCAAACCAGTTGCTTAAGGGCAC +AGTTTTGTTCTTTACCTATATTTTTAACAAATATTTTATGTAAGTAGTTGACAAAATCAA +ATACTGTGTACACTACCGAGGCTTCCCTGGGAAAGCCATCAGCCTCTGCCCCATCCCTTC +CCACTCCTGATTCCACTTTCCTGTGTTTCCATATCTTTTTCATGTCTGTTTCTGGCCCAC +AGTGGGCGATCAATACATGTTAGCCACCAACCATCAAACCTATATTGAGTAATTATGGTA +TGTCAGGCACTATGCTCAATGAAATTGTAttaggcttgtacaaaagtaattgtggttttt +aagagtaatggcaaaaacggcagttactttcgcaccaacTATTTGCTGCCTTGAATTATT +CCTCCTCTCCTCATCCCTAAACCCTGCTCCTCCCAGCCATTCTTCCTCCCCTTCTTGGGC +CATGGCCAGGCCCCACCCAGGTACTAAGACTCAGGTGAACCAAGGAAGACTTAATGCCCA +CTCTTTTCTGATGCCCATGTTGGCATGTGTTAAGtcggttagcattaagtttggctgcat +ttagcagagacccaaaagaacagtgccttttaaaaggcagaggttatgtctctcacacac +acccagcacaagtccaagaccagcatggcatctcagctccatcaacctcaggaaccgagc +tcctgcagctccctgccctgcagttgataaggtgaggtctttgtcctcctggttcaagat +ggtgctagaatgttggctaccatatctatagtccaggcatcagaatggagcaagggatga +aaaaggaagagatgaaggcacacgacaggttcctgagagctggcacaggacacttctgct +tatatttcactggccagaacttagtcacatggtcacacctagttgggagactctgagaag +taaagtatttattctagatggccatatccctacctaagacttggagttttctatgactgg +ggaagaacggaagacaagatattgggaaagactagcagcctctactaAAAGGGTGATCtg +tgttgatgtgcgtgtgtgtgtgatgtttgtatgagcatgtgtgttatgtgttgtgtgtTG +GTGGGGCAGATTCTTGCGAGCACTTTGGTCTCAGATGGACCTGCTACCAGTTCTCTCTGC +AGACCCCCATAGGTTTCTCCTAAACCTGGCCTCTCCTATTAGGCAGCCTTACTCAGCGGC +AGCTTCTCAGCTCCATGTTTTCAAGGAACCACAATTTATTTCCAGCATCCACTGAAGCAT +ATTATCAGTGGTGATAGAGGGGGCTTGTAAAACTGTTTTTCCACTTAGGTATTAGAGGGT +GGCCATTATTTGAGAGTGACTATGACCACAGTTAATCTGGTAATAAATTCTCTTGGGTAG +GAGGGGGAAAGGAAAGGATGCTTTAAGGAAGCATCTTGCCAGGAGACACAAAGCTAACAA +GAGTGGAGCCTGCAGCTGGAGCCGCAGAGCCTAATCACTACACCCGCCCATCTCTGCTAG +GGTTTCATGACTTCGTATCGGGGATTAGCAGTATTTAACTCTGTTGCACAAACATTTGGT +GTATTATTCAGGTAACAAGTAGCTAATAGAGGAAGTTTTACTTTTTTAAGACATAAATTT +GCCTTTTCCCAAATTACTTGGTACATAGTACTTTTCATGTTTGAAGTTGAGATGTGGGTA +CAATACCATAGCTTTATTCCAGAGCAGGGTATTTGTTTCCAAATGCCATGTTCCCAGCAG +CTGCCCTTGACTGGGAATTGGGGTGTGATTTGGGCTTTTCCTTAAATCCTTGAGGAGCTG +GAGGGGTGGGTGGCTCGCACTCCTGCTTTctggatctgaatcctgactctgtcatggacc +tgtttgactttgggcaagttgactcctattcctgagccccatatttttctcttctgtgaa +attcagattaaaaAAACATGGCTTTGATCAAACATTATAAATAATATATAGACAGACTGC +TTGTTTTTATTGTATTGCCAGAAATGAATCCTACTAATATTGCCATCTATGGACAGAAAA +TGTATTACCTGTCTTCATCAAGACCCAGACGAGGAAGAACACGAAAAGCGGAGATTAATT +TTACTGCCATCTCCAGAACCGTCATCCTAATATTTACTTACATTTTATTATTATTTCAGG +CTCATGCACATATACTTAGCATGGATCATTGGCCACAGACTCGCATACATTTAACTTTAT +TACCTTTTGCCTCATGTATCTCATTAAAATTTTGCTGCTTAATCAAGGATCTGCATATTA +TTTTAATTTTAGAATTCACAGTTCCAAGACTTTGAAAGTTTCAAGCGTTCTGGGTGaatg +tgttatgctctctcccgccaccatgtctttataccccctgatttctcagccactatggca +accactttctactcttagtagcccatatttagtccaatccccagctcaggagacacttct +tccagggagccccctgtgccttccagtagtatcttgtacctgccctttttgcaaagctct +ttcctcctggcttagaatggcccattgacctgtttgtttctcctattaaactgtaagcca +ctcgagggtagagagcatctgttgttcaccattgcatcctcggtgctgagcactgcgtct +gacatattatttagaaggtcagtaagtgctagtgggatTCAGGCTCCCAGTGGGTGGGAG +AGAAAGGACGTAAGGAAGCAAGTGGTAAAGGCCCTCACAGAGTATCAGCAGGCTGGTGTG +AGGGAGAAATGCAGAGGATGGGTGAGTAGCATAATCGCTAATGATAGGGTAATGATAGAG +CACATTTCACAACACCTTtaagccctttcacgtgcatcagataatttgatcctcataaaa +gcctagagatagatatattacagggatgaaggtggagtattttgtggttatgtgatatgt +ttaaaattatgcagtgagtaaatgactgggttcaaaccagaccttaaaagtctgttatct +ttccCTCGAGCATGCAATGAAGTCTACATCATCCCTACCATGTCCATTTGATCACACCCT +GGCCTCACAGCTCTGTGGTCTACAGGATACCTCATGGTGGTTTTATTGACCAGACAATAA +TCCTCTTTCTAAGGGGATGCATTTCATTAATACATATGTAGATCATGAATTGTCTTTGAC +TTTGAGGGGATGGTAGCCAGAGCAGAAAGCAAAGCTGATTTTCATCCCCGTCTGGTAATG +TGGTTGGTAATGTGAAGATGGGTGTATTCTGAGATACCGGCTCCTTGCAGTGTGTGGTTC +CTTCTGTTTTCAGGCCCAAGAAGCCCATCCTGGGAAGGAAAATGCATTGGGGAACCCTGT +GCGGATTCTTGTGGCTTTGGCCCTATCTTTTCTATGTCCAAGCTGTGCCCATCCAAAAAG +TCCAAGATGACACCAAAACCCTCATCAAGACAATTGTCACCAGGATCAATGACATTTCAC +ACACGGTAAGGAGAGTATGCGGGGACAAAGTAGAACTGCAGCCAGCCCAGCACTGGCTCC +TAGTGGCACTGGACCCAGATAGtccaagaaacatttattgaacgcctcctgaatgccagg +cacctactggaagctgagaaGGATTTGAAAGCACAGGGCTCCACTCTTTCTGGTTGTTTC +TTTTGGCCCCTCTGCCTGCTGAGATTCCAGGGGTTAGTGGTTCTAATTCTAAACCACTCC +AAGAACATTTGATTTTGCTACATGTTTCCATTTAAAAATCATAGGATTTGggctgggtgt +ggtggcttgtacctgtcatcccagcactttgggaggccaaagcaggaggatcattcgagc +ccaagagttcgagaccagcctgggcagcatagggagaccccatctctacaaaaataataa +aaaatgttagctgggcatggtggtgtgtacctgtggtcccagctaggggaggctgagatg +gaaggatcacctgagcctgggaggttgaggctgcagtgggccctgatcatgccaccgtgc +tccagcctgggtgacagagtgagaccttgtctcaaaataaataaataaataaataaaAGT +CATAGGATTTgatcaggcatgatgggtcacatctgtaagcccattgctttaggaggccaa +ggtaggaggatcagttgaggccaggagttcaagaccagcctgggcaacatggcaagacct +ctctctctaatttttaaaaaaataaaaaTTAAAGATAAGAAAAAAATCATAGGATTCTCA +TGAGGCCTCACGTGCTTATTTTCAACCTACCAAGGGGAAACCCAGGCCTCAGCGATTAGC +TGAGCCACATGCAGGCACAGCCACTGTCTCTTTCCTTCCTGTCCCCTCTGTCCCCACCTT +CTGCGCTCGCCTTCCTCCCTGACTTCACTTCCTTGAATCTTAGTGCCTACGACCAGAGGG +AGCTGTGAAGTTCCTTGTGTCCCATTGGCAGGAACAAGACCCCCAGAAGCATCTCCTCAG +GGCCTCTATCCCATCTCTAGATGTGCTTGTCATTAGGGttcttgtagttccagctgatct +ctggccctgccgctcaaagatacccaaaagagcgagtctaccctttttcacattcaaccc +tctactgatttgcaaatagcagtcagtgcccaccctggtcttttctctggggtccagcag +gcctagaccttcagccattttcctgatgaGGTCTGTAtttgaaattaggaagattaagtt +tgaatcttcacacttctgatgtctgtgagatcttcagcaagttccttactgtctttaagc +cttgttttcatcatctggataatggggatatcacacactattcacaaggttgttatgagg +cctaaattagctaaagcaATTGAATCCTCCTTACCCCCTGCATGGAGCTCTCTGGAGACT +TCCACGTCTCCTGGTCATTGTGGGTGTCTTATGGTAGTCTTGGGCAGTTAGGGAGAAGTT +AGGTGTCTGGAAGCAAAGATGGCTCAGAACTAGATAGAGTCTTGGGCATTTTATAGATAA +AAACTCTTGTCTCCtttaaaaataataaaaaaaaattaGCTGGGCATATTAGCCACTCAG +CAAGACTGCACGTGATAGATCCCGAGTGCCCCACCTTGGGTGGTGTAATACACAATATCA +CGGGAGCCCCGGGTAGTAACCACGGAGGTGTCAGCCTCAGTGCTGTGGGCAGATGGATGG +GGAGAGCCTCCCGGAACTGGAGTCACTGGAGCAGGGTTGGGGGGCCTCACTGAGGGTACG +GCCTTGATCTCTAAGGAGGAGGGACTGCCTGGAAAAGCTGACTGGGAGGGAGGACTCGGC +TGGGGGTAGAAGGGACTAGGGAAGGCTGGGGGTGGGGGTGCTTATGGAGGACCTCAGATG +CCTGGGGAACAGACTCCACTAAATAAAACATATGAAACCATGGCTGGTTCTTCAGCAGAG +GCCATGTAGAGAAAGGAATGACCTAGGAAAGTTGGCCTGGAAGTGGAGGGAAGGATGGTG +TGGGAAAAGCAGGAATCTCGGAGACCAGCTTAGAGGCTTGGCAGTCACCTGGGTGCAGGA +TACAAGGGCCTGAGCCAAAGTGGTGAGGGAGGGTGGAAGGAGGCAGCCCAGAGAATGACC +CTCCATGCCCACGGGGAAGGCAGAGGGCTCTGAGAGCGATTCCTCCCACATGCTGAGCAC +TTGTTCTCCCTCTTCCTCCTGCATAGCAGTCAGTCTCCTCCAAACAGAAAGTCACCGGTT +TGGACTTCATTCCTGGGCTCCACCCCATCCTGACCTTATCCAAGATGGACCAGACACTGG +CAGTCTACCAACAGATCCTCACCAGTATGCCTTCCAGAAACGTGATCCAAATATCCAACG +ACCTGGAGAACCTCCGGGATCTTCTTCACGTGCTGGCCTTCTCTAAGAGCTGCCACTTGC +CCTGGGCCAGTGGCCTGGAGACCTTGGACAGCCTGGGGGGTGTCCTGGAAGCTTCAGGCT +ACTCCACAGAGGTGGTGGCCCTGAGCAGGCTGCAGGGGTCTCTGCAGGACATGCTGTGGC +AGCTGGACCTCAGCCCTGGGTGCTGAGGCCTTGAAGGTCACTCTTCCTGCAAGGACTACG +TTAAGGGAAGGAACTCTGGCTTCCAGGTATCTCCAGGATTGAAGAGCATTGCATGGACAC +CCCTTATCCAGGACTCTGTCAATTTCCCTGACTCCTCTAAGCCACTCTTCCAAAGGCATA +AGACCCTAAGCCTCCTTTTGCTTGAAACCAAAGATATATACACAGGATCCTATTCTCACC +AGGAAGGGGGTCCACCCAGCAAAGAGTGGGCTGCATCTGGGATTCCCACCAAGGTCTTCA +GCCATCAACAAGAGTTGTCTTGTCCCCTCTTGACCCATCTCCCCCTCACTGAATGCCTCA +ATGTGACCAGGGGTGATTTCAGAGAGGGCAGAGGGGTAGGCAGAGCCTTTGGATGACCAG +AACAAGGTTCCCTCTGAGAATTCCAAGGAGTTCCATGAAGACCACATCCACACACGCAGG +AACTCCCAGCAACACAAGCTGGAAGCACATGTTTATTTATTCTGCATTTTATTCTGGATG +GATTTGAAGCAAAGCACCAGCTTCTCCAGGCTCTTTGGGGTCAGCCAGGGCCAGGGGTCT +CCCTGGAGTGCAGTTTCCAATCCCATAGATGGGTCTGGCTGAGCTGAACCCATTTTGAGT +GACTCGAGGGTTGGGTTCATCTGAGCAAGAGCTGGCAAAGGTGGCTCTCCAGTTAGTTCT +CTCGTAACTGGTTTCATTTCTACTGTGACTGATGTTACATCACAGTGTTTGCAATGGTGT +TGCCCTGAGTGGATCTCCAAGGACCAGGTTATTTTAAAAAGATTTGTTTTGTCAAGTGTC +ATATGTAGGTGTCTGCACCCAGGGGTGGGGAATGTTTGGGCAGAAGGGAGAAGGATCTAG +AATGTGTTTTCTGAATAACATTTGTGTGGTGGGTTCTTTGGAAGGAGTGAGATCATTTTC +TTATCTTCTGCAATTGCTTAGGATGTTTTTCATGAAAATAGCTCTTTCAGGGGGGTTGTG +AGGCCTGGCCAGGCACCCCCTGGAGAGAAGTTTCTGGCCCTGGCTGACCCCAAAGAGCCT +GGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAAAGGGCTGAAAGCCA +TTTGTTGGGGCAGTGGTAAGCTCTGGCTTTCTCCGACTGCTAGGGAGTGGTCTTTCCTAT +CATGGAGTGACGGTCCCACACTGGTGACTGCGATCTTCAGAGCAGGGGTCCTTGGTGTGA +CCCTCTGAATGGTCCAGGGTTGATCACACTCTGGGTTTATTACATGGCAGTGTTCCTATT +TGGGGCTTGCATGCCAAATTGTAGTTCTTGTCTGATTGGCTCACCCAAGCAAGGCCAAAA +TTACCAAAAATCTTGGGGGGTTTTTACTCCAGTGGTGAAGAAAACTCCTTTAGCAGGTGG +TCCTGAGACCTGACAAGCACTGCTAGGCGAGTGCCAGGACTCCCCAGGCCAGGCCACCAG +GATGGCCCTTCCCACTGGAGGTCACATTCAGGAAGATGAAAGAGGAGGTTTGGGGTCTGC +CACCATCCTGCTGCTGTGTTTTTGCTATCACACAGTGGGTGGTGGATCTGTCCAAGGAAA +CTTGAATCAAAGCAGTTAACTTTAAGactgagcacctgcttcatgctcagccctgactgg +tgctataggctggagaagctcacccaataaacattaagattgaggcctgccctcagggat +cttgcattcccagtggTCAAACCGCACTCACCCATGTGCCAAGGTGGGGTATTTACCACA +GCAGCTGAACAGCCAAATGCATGGTGCAGTTGACAGCAGGTGGGAAATGGTATGAGCTGA +GGGGGGCCGTGCCCAGGGGCCCACAGGGAACCCTGCTTGCACTTTGTAACATGTTTACTT +TTCagggcatcttagcttctattatagccacatccctttgaaacaagataactgagaatt +taaaaataagaaaataTGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCC +ATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCC +ATCCAGAATAAAACGCAGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAAATGACC +CCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCATGAC +CCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGAC +CCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGAT +GCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGAT +GCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGAT +GCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGAT +GCTTTGCTTCAAATCCATCCAGAATAAAACGCAGATGCTTTGCTTCAAATCCATCCAGAA +TAAAACGCA
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/emboss_needle_out.markx10 Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,866 @@ +######################################## +# Program: needle +# Rundate: Mon 20 Jan 2025 11:47:46 +# Commandline: needle +# -asequence /tmp/saskia/tmpsg97cix0/files/8/d/8/dataset_8d81948c-002a-4a33-ae33-6042793fd219.dat +# -bsequence /tmp/saskia/tmpsg97cix0/files/f/b/9/dataset_fb94eaec-e786-4645-99b5-6936b8d9a907.dat +# -outfile /tmp/saskia/tmpsg97cix0/job_working_directory/000/15/outputs/dataset_6b349e2c-cff0-4ae4-a35c-c035f1bcf9bd.dat +# -gapopen 10.0 +# -gapextend 0.5 +# -brief yes +# -aformat3 markx10 +# -auto +# Align_format: markx10 +# Report_file: /tmp/saskia/tmpsg97cix0/job_working_directory/000/15/outputs/dataset_6b349e2c-cff0-4ae4-a35c-c035f1bcf9bd.dat +######################################## + +#======================================= +# +# Aligned_sequences: 2 +# 1: FC12044_91407_8_200_406_24 +# 2: hg17 +# Matrix: EDNAFULL +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 20141 +# Identity: 21/20141 ( 0.1%) +# Similarity: 21/20141 ( 0.1%) +# Gaps: 20116/20141 (99.9%) +# Score: 60.5 +# +# +#======================================= + +>>>FC12044_91407_8_200_406_24, 25 nt vs hg17, 20141 nt +; mp_name: EMBOSS +; mp_ver: 6.6.0.0 +; pg_name: needle +; pg_ver: 6.6.0.0 +; pg_matrix: EDNAFULL +; pg_gap-pen: -10.0 -0.5 +>>#1 +; sw_score: 60.5 +; sw_ident: 0.001 +; sw_overlap: 20141 +>FC12044_91407_8_200_406_24 .. +; sq_len: 25 +; sq_type: D +; al_start: 1 +; al_stop: 25 +; al_display_start: 1 +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +----GTTAGCTCC-------CAC------------CTTAAGATGTTTA-- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +-------------------------------------------------- +----------------------------------------- +>hg17 .. +; sq_len: 20141 +; sq_type: D +; al_start: 1 +; al_stop: 20141 +; al_display_start: 1 +gtttgccatcttttgctgctctagggaatccagcagctgtcaccatgtaa +acaagcccaggctagaccaGTTACCCTCATCATCTTAGCTGATAGCCAGC +CAGCCACCACAGGCAtgagtcaggccatattgctggacccacagaattat +gagctaaataaatagtcttgggttaagccactaagttttaggcatagtgt +gttatgtaTCTCACAAACATATAAGACTGTGTGTTTGTTGACTGGAGGAA +GAGATGCTATAAAGACCACCTTTTAAAACTTCCCAAATACTGCCACTGAT +GTCCTGATGGAGGTATGAAAACATCCACTAAAATTTGTGGTTTATTCATT +TTTCATTATTTTGTTTAAGGAGGTCTATAGTGGAAGAGGGAGATATTTGG +ggaaattttgtatagactagctttcacgatgttagggaattattattgtg +tgataatggtcttgcagttacacagaaattcttccttattttttgggaag +caccaaagtagggataaaatgtcatgatgtgtgcaatacactttaaaatg +tttttgccaaaataattaatgaagcaaatatggaaaataataattattaa +atctaggtgatgggtatattgtagttcactatagtattgcacacttttct +gtatgtttaaatttttcatttaaaaaaaaactttgagctagacaccaggc +tatgagctaggagcatagcaatgaccaaatagactcctaccaactcaaag +aatgcacattctCTGGGAAACATGTTTCCATTAGGAAGCCTCGAATGCAA +TGTGACTGTGGTCTCCAGGACCTGTGTGATCCTGGCTTTTCCTGTTCCCT +CCGCATCATCACTGCAGGTGTGTTTTCCCAAGTTTTAAACATTTACCTTC +CCAGTGGCCTTGCGTCTAGAGGAATCCCTGTATAGTGGTACATGAATATA +ACACATAACAAAAATCATCTCTATGGTGTGTGTTGTTCCTGGGGTTCAat +tcagcaaattttccctgggcacccatgtgttcttggcactggaaaagtac +cgggactgaaacagttgatggcccaatccctgtcctcttaaaacctaagg +gaggagaTGGAAAGGGGCACCCAACCCAGACTGAGAGACAGGAATTAGCT +GCAAGGGGAACTAGGAAAAGCTTCTTTAAGGATGGAGAGGCCCTAGTGGA +ATGGGGAGATTCTTCCGGGAGAAGCGATGGATGCACAGTTGGGCATCCCC +ACAGACGGACTGGAAAGAAAAAAGGCCTGGAGGAATCAATGTGCAATGTA +TGTGTGTTCCCTGGTTcaagggctgggaactttctctaaagggccaggta +gaaaacattttaggctttctaagccaaggcaaaattgaggatattacatg +ggtacttatacaacaagaataaacaatttacacaattttttgttgacaga +attcaaaactttatagacacagaaatgcaaatttcctgtaattttcccat +gagaactattcttcttttgttttgttttgcgacAGGGTTGCGCtgatcct +cccgcctcagtctccctaagtgctgagatgttgcaggaagtcagggaccc +cgaacagagagatcggctggagccgtggcagaggaacataaattttgaag +atttcattttaatatggacacttatcagttcccaaataatacttttataa +ttttttatgcctgtctttgctttaatctcttaatcctgttatcttcataa +gctaaggatgtacgtcacctcaggaccactgtgataattgtgttaactgt +acagattgattgcaaaacatgtgtgtttgaacaatatgaaatcagtgcac +cttgaaaaagagcagaataacagcaatttttagggaacaagggaagacaa +ctataaggtctgactgcctgcggggtcgggcaaagggagccatatttttc +ttcttgcagagagcctataaatagacctgcaagtaggagagatattgcta +atttcttttgctagcatggaatattaatattaacaccctgggaaaggaat +gcattcctggggggaggtctataaatggccgctctgggaatgtctatcct +acgcaatggagataaggactgagatacgccctggtctcctgcagtaccct +caggcttactagggtggtgaaaaactccgccctggtaaatttgtggtcag +accagttttctgctctcgaacactgttttctgttgtttaagatgtttatc +aagacaatacgtgcaccgctgaacacagacccttatcagtagttctcctt +tttgccctttgaagcatgtgatctactccctgttttacaccccctcacct +tttgaaacccttaataaaaaacttgctggtttgaggctcaggtgggcatc +acagtactaccgatatgtgatgtcacccccggcggcccagctgtaaaatt +cctctctttgtactctctctctttatttctcagccagctgacacttatgg +aaaatagaaagaacctacgttgaaatattgggggcaggttcccccaataT +CTGGTGCCCAACGTGGGAtactgagattacaagcatgagccactgcatct +ggcctcttcttttgatttttttttttcaaacttttacaaatgtagaaacc +attcttagcttttgggcattaccaaacccggcagtggcaggctcggttca +ccaacgtcatttgcagttccccgCTTTATGTTATGGgttttgttttgttt +tgttttttttattgagacagagtttcactcttgttgcccaggctgtagtg +caatggtctgatcttggctcactgcaacctccacttcccaggttcaagcc +attctcctgcctcagcctctcaagtagctgggattacagacactcaccac +cacacctggctaattttgtatttttagtagagatgaggtttcaccatgtt +ggccaggctggtctcgaaatcctgacctcaggtgatccacccaccttggc +ctcccaaagtgctgggattacaggcttgagctaccacgcctggctGGGTT +GGTTCTCAATGGAGTGGTTTGTTTTTGGAGCTGCTCTGCGCAGtggggac +cagaataggcctgggttcctagcccattgctattccttaccagctgtgga +ttctaaggaaagtcatttaacctcgctggaccttagattcctcatccctg +aaGCCCAAGGGTaaaacaaaacaaaacaaaacaaaacaaaccaaCCCATC +ATGTAAAGCGGGGAACTACAAACGATACAGGTGAAACATGCCTACCACAC +CACTCACAGGCTATGATGACAAAAACGTGGCTACATCTGGGACCACCCCC +CAACCCCCACTTTGTACGTAGGAAATACGGAGTTGAGGATGGAGACCCAC +AGTATGTCCAGAGTGTCCCCAAAGGCCACAGTGCCCGCCTGGAGCCCTCC +AGAGAGCGTGCACTCCCTGGGGTGCCAGCCAGAGACAACTTGCCCTGAGG +CTTGGAACTCGATTCTCCGCGTGCCAGAGAAGGGGTGGGACTTCAGAACC +CCCAACCCCGCAATCTGGGTCGGGGAGCCTGGCGCACTGCGGGCCGCTCC +CTCTAACCCTGGGCTTCCCTGGCGTCCAGGGCCGTCGGGGCCGAGTCCCG +ATTCGCTCCCACCCCGAAGCCGCGCCAGGACCAACGAGGGCGCAGCCGTA +TGCCCCAGCCCGCTCCGCGGAGCCCCTCACAGCCAcccccgccccgaccg +cgccccgcgcggcTCGAAGCACCTTCCCAAGGGGCTGGTCCTTGCGCCAT +AGTCGCGCCGGAGCCTCTGGAGGGACATCAAGGATTTCTCGCTCCTACCA +GCCACCCCCAAATTTTTGGGAGGTACCCAAGGGTGCGCGCGTGGCTCCTG +GCGCGCCGAGGCCCTCCCTCGAGGCCCCGCGAGGTGCACACTGCGGGCCC +AGGGCTAGCAGCCGCCCGGCACGTCGCTACCCTGAGGGGCGGGGCGGGAG +CTGGCGCTAGAAATGCGCCGGGGCCTGCGGGGCAGTTGCGCAAGTTGTGA +TCGGGCCGCTATAAGAGGGGCGGGCAGGCATGGAGCCCCGTAGGAATCGC +AGCGCCAGCGGTTGCAAGGTAAGGCCCCGGCGCGCTCCTTCCTCCTTCTC +TGCTGGTCTTTCTTGGCAGGCCACAGGGCCCCACACAACTCTGGATCCCG +GGGAAACTGAGTCAGGAGGGATGCAGGGCGGATGGCTTAGTTCTGGACTA +TGATAGCTTTGTACCGAGTTCTAGCCAGATAGAAGGTTACCGGGAGCTGG +GGAGCGTTGGATTTGCTGCTGGGCTGTGCCGGTGCCCAGAAGGCAGGACC +TTGCAGAACCAGCCAGGTCCCTGGGAGACTGTCAGACCCACCAACCTGGT +GGCATTCGCAGAGCTGAGATGCATTGGAAATTGCCTTGGGCACATCCCCA +AAGATCAGGATGTCCCACCCCAGTCTGAAGGAGATAAAGTTGGGGGTAGG +AGAGACGCAGATGCAAGTGATCAGTCTCAGTCCCAGACATTGCCTTGCTC +TGCGGGTAGGAATTCAGGATTCATTTTCCAGGGAAGTTCCTGACCTCTGA +ATGAGAGGGGCTGTGTAAGGCCAATGCCTGGGAGGAAGGCAAGGATGAGT +AGAGGTGGGGGGAAACAAGTGTCAGGAAGACTCAAAATCTTCCAGAGAAA +TTGTGCAGGGTCTTACCAGATCTGTCCTCAAAGCCATGCAAATTGCCTTC +TTTGCAATGCATACAATGAGGTGTCTCTGGGGGTCAGAACTGGTTATTAG +GGAACTTCTAGCCAGGACTGCTAAATACGCGCTGTTGGCCCACCAGGCTC +ACCTATAGCCTTCCTTCAGTCTGGGCTTGGTTTGGATTTCACTGTGGGTG +CCATCGCCTTTACACTCCTGTTTCTATAGTTTAAAGATAGTGGTGCTTTG +GGAAAGTGACTCCTTAAATACAGTTAGGTCCAAGTGAGACAAGTGGCCTG +GCTGTCATTTCAGAATAGCAGCTTCCAAGAGGTGATTAATTTCTGTTGGA +AGGGTGATCTTTGGGGAGGTGGGTGAAGAGCAGAGACTTGGTGGTACCGT +TCCAGGAGCACAGGCTCTCTTCCTTTGCAGTGCAGAATGACCTCTGGCAG +CCGGAGTTGTGTTTGTTCTGTAGGATTCTGAGGTGGGCCATGGGCAGCTG +GAACTGGGGAATTTTGCCAATCTCTTTCATATTAGGATTGTCTGCAGAAC +CAGATATGGAGGCTTCTAGCAACGTGAGTGCTCCTGTTCTAATGCCCTTA +GAAACAAGAAGGCCACACTGATCATTTCTCTCACTTAGGCAGGGAGACAA +GGCAAGAGAGAAACAGTGGATGCTTTTAGGTTCTTTCCCTTCCCAAGCAG +TTGTGGACATTGGGCTGAGGGGAACATTTCCACATTGGCTAAAGGAGCGT +CCTCCTCATATTTTGTACATTTTATACCCAAAATAACTCTTCTTGGTATT +TGGGGAAATATTTTCCTCCCCGTCCATTCCAGGAAATGGCTCCAAGTGCC +AAGGACAGAGCCAGGGAAGTTGCAATGAATTCCTGCCCGTCAGCCCCAGG +CAGATGCCTTGCACGTCTGAGTGGCCCATGCAGAGCGTGGAGGTGGCCGC +CACGGAACCTGGGTCAATGTCCCACCCCCGCTTAGATGCCACCAGGGGCG +TGGGAGCCAAGGAGAGAAGAGGGGCTCCAGGAAGGTAGAGTCCTTGTGTC +TTGTGCATCTGTGAACAGCACTGGTATGATTTAAAGGAAAATTGAGCCAA +ATTTTCCGGCAGTCAGTTACCCCATCCCCACCGGGGTAGGAGTCTGGCAG +CCGCAGCTCCATTCTGGCCAGTCGGCAGAGAGCCTTGAAATTCTTCTTTG +TCCACACAGTTGTCTCAGAGAAACAGAGAGGTTGTTTCTGCTTAAAAACA +ACACACTTGGTGTCTGGGCCCACAGACTCCTTTGCACTTATTCCACGTGT +GACAGCCAATGTGCCTCGTTGCTTAGCAGACAGCATGTTACCGTCTTTCC +TGCTCAGTTTGTTAGCTCTATGGAATGGAATTTATAATCAATGCCCATAC +CAACATTTCACTAATATCATAGGAGATTTAGTCTCCATCTGGGTGTACAT +TACATTTGCTCTGGGGTGCTCCAGGCTGGGGGGTTGCCAAGGAAGAGAAG +AGAAACCGCAGAGAAGACGGGAGGGCAGGGCAGGGGTCTCTGAGAAGGGG +AGGGGTCCCAGAGTGCAGGAGCAGGAGCCAGGCTCATGAAAGGGGCCACG +GGCGGGAGTATCCAGGGACGGCAGTCAAGATGGAGCACAGCTTAGGAAGC +TGAAGGGAATCCTGGCCCACCTGGGTGCTAGAGGGCACATAGGAAGTGCA +GGAAGCAGACCAAGGTCCCCAAGAGAGGGAGACCTGGACGCTGAAGCATT +TTCTGTCTTTATTAAGACAACTCCGTAAGAATTCCTGCTGGGCCAAAGTG +AATTCTAGGATGCGACTTTAAGATGGGAGCAAGCGAACCATTGAGGAGGC +AGGTTACCCTAGTTAGCCAATGCAGATCGAGAATGGGAAATCTTTCattt +attcatgcaacagatatttaacgaagccctgccgtgttccaggcctgtga +tagatgctggaacaggtacagagatAcaggtgtcattaattgatcagggc +aacctctccttctgagtcttgctggagcttcagatgcccctcacacagag +ctcgagggagcctcaacaattgatcagaagtcaggcaccatggctcacgc +atataatcccagcactttgggaggccaaggcaggtggatcactggagccc +aggagttccagatcagctggggcaacatggcaaaaccccatctctattaa +aaaaaaaaaaagtaactggatgtgatggtacacacctgtagtcccagcta +cttgggaggctgagaggtgggagaattgcttgagcccgggaagtcggggg +tccagtgagccttgatcacaccactgcactccagcctgagtgacagagca +agaccctgacacacacacacacacacacacacacacacacacagattaga +gctgaaacaggagtagaaacctatctgtatctctgATGAGATCAGATCTT +TCTGATGAACAGAAAGAATGTAACCCCTGTACTCACACCCTCTCTGCTGG +TTACATATGTTAACACGATTTCTCAAATGAGGCTTTTGGTTGCAAATAAG +AGAAAATCACTCACGCTGGCCCTGTGTTTTTCAAATTGTTTATTGTGATC +AACATTTGAAAAAAGAGCCGAGACTCTCAAGAGTGCATTACCCACGGTAA +GGGTGAATTTTACTTCTTGACACTTATTTCTCTTACATGTATCTATCTGT +CTCAAATGAAAAATATATTTAGAAAGTTGAAAGCTATCCAAGTGAGTATA +AGAAAAGAGTATCTCACCCTGAAGGCTAAGGACAGGGAGGGCCACCAGGC +CTCACGAGGACCCAGGAACCACAAAGAAGGCTAGGAAGGAGCACAGGCGG +TGACCATACTCTGGCTCAGTGGCTATGTGGGCTCTGGTCTCTCTCAGCTG +TTCCATGCATATGAGGCCAAATGTGGCTACCCTAGAGCTTCTGAGCCCTC +AACAGAGATGAACTGGACTCTCTGCAGCCCCACTCTAAATTCCTAAGAGA +GAAGTTGATTGACCCAATCAGGGTCAGGAGAAGGAAGGGAGGAGGAAAGG +GAGGAGAGAAGAGCCTCTTCGTCTCTTGCCTACCACTGGCCAGGCAATTG +TAGCCAAGGGGGCTGGAGTGTAAATGCAAACATAGCCATCAAGGGTtgtg +tatgtgtgtgtgtgtgtctgtgtgtgtgtatgtgtgtCTCTTGGGTAGGT +TAGATCTCCCAGGAGGTCCCTACTAAACAGACTTAAGCCCGCAAAATTTT +AGCTCTCCAGCCTCACACACTCCACCCCTCTACCATATTGAATCTTCCCA +AACCAACTATGGCTTTCCCTAACTCCGGAGcttggcctggaatgccctgc +ttcccctctttcccctggggaacgcctgtccttcaggcctcagttcacac +actgcctcccttgcaaagctctccTCCCATCCCCGGAGTCCCTCTTCCCC +TTTGTTCTTTGGGTTCTATGCTTCTTCCCTCATAACTCCCACCAGGTTGT +GTTAAAATGAGTTGTTCAAGGTCCTGTCTGTTCCACTAGATTCTGAGCAA +CTTGGAGAACGAAGATCCAAACTTCGCTGCCTTTATTTCCTCCTTTGTTC +TTTTCTCATCCCCAAGTCCCTTCCAACTTGGAGTTATgaagaaaggaagg +aaggaagggtgggagggaagaaCAGGAGGGGATCCCACAGGAGAATGTGT +ATAGGGAGAGGACTCAGACTAGCTAAAGCTTTTCCCTCATAATTAATAGC +AAATACCATGTTACCTGAATTTAATTCACAGTAGCATACAAAAGACTCGC +TTTGTTCTCCCCATTGATGTCATCAGAGGGCTGTGGGCAGGCCTAATCTT +GGCTCAGGAGGCCCTCCAGCCTGGATCTAAAGAGCAGCAGATGggccagg +ctcggtggctcatgcctgtaatcccagcattttgggaggccgaggcgggt +ggatcacgaggtcaggagtttgagaccagcctggccaagatggtgaagcc +tcgtctctactaaaaatacaaaaattagccaggtgcggtggtgggcgcct +gtatttccagctacccgggaggctgaggaggctgaggcaggagaatcgct +tgaacccgggaggcggaggttgcagtgagccgaggtcacgccactgcact +ctagcctgggcaacagagcaagactccgtcaaaaaaaaaataaaaaaata +aaaaaataaaaaaaataaaGAGGAGCACACATCTCTGCCCATCCTAACTC +CCACTTTGACATTGAGGTCCCCAGGATGGAGGGTCTGCCTCCATCTGCCT +TGTCCCCTGCAATGGTGGGAAGGTGATGGAGCTCAAGTCTAGAGGCCACC +AGCTTCTTAGGGAGGTAGGAGGTGGAGGGTGGGGTGCGGGCCCTGCACAC +AACTGCCAAGTGAGGATGGGGGTGGGGTCCACCTGAGGATAAGTAACAGT +GAGGCTGGTGCAGAGGACCCAGGTGGAGGTAGACAGCAGAATTTGTGGTG +GGGTGGATGGCACATTATATAAGCCTCTCTTGCTGCCCTGTTTACTGAGA +TTGTTTCAttatcttttttggcttttgtttttaagagatggggtcttgct +gtgtcacacaggctggagtgcactgtgtgatcatacctcactgcagcctc +gacatcctgggctcaggcaaacctcccaccttggcctcccaagtagctgg +gaccacaagcgtttgccaccacactcagctatttttatttttatttttat +tttttttagagatggggtcttgctgtgtcgcccaggctggtcttgaactc +ctgggctcaagcgatcctcctgccttggcctcccaaagccctgggattat +aggctgagccaccacacccagccACATTTCATCTGTGCAGCTCCAGGGGC +TCCACATTCTACTCTTCTCATTTCTTCTCCAGGGTACCCATGGCAAGGGA +TGAGGGTAGAAGATGGGGCAGCCAGGCCTTGATTAAAGGAGAAGGAAGGC +AGCCTGTGGAGAGGGCAGCCCAGGGAGTGCAGAGAGAAGTGGGCCATGAG +GGAGACAGCAGAGTGCAGGCTGCGTCCCAAATGAGCATACAGCCCACTGT +GAGCCCACCATCTTCCTAGAGACCCCTCTCCTCTCCAGGAGCTGCTTCAG +TAGCACTCAGAGGAAAGAATGATGCTGTATCAACATTTCAGCAGCTCATC +TTTTAACTCTAAGAAAATGGCAGCTCCTAAATGTTCAAAACTGCTTTGGA +AACTTCTGGAGAGAGGTTTTGCAGCTCAGGCAGACAGCTGATCGCGGCCT +TTCTTCCACCCCAACCCATGCTCTCCCCATGCTCTCCTGCCACAGCTGCA +GCGGGCCCCTGGGTCCTACATTTGCAGCCCTTTGTCTCTGAGCTCAGACT +TCCAATTCCAAGCGGCAGCTGGGCAGGCTCACCAGCATGTCCAGCCAGTA +CTAGGACATCAGCAGGAGCCCAACCACCTCTTTCCAAAATCTCTCCTCAT +GTCTCTCCTAGTTTCCATCTCCATCCTTCTAGTCAGCCAGGCTGAAAACA +TTTGCTCCTCAGGGTGCAGAAGGGAAAGCTTTGCCTCCCTTCCTGGTGCT +CACTGCCCCTGCGATTCCAGCCCAAGCCCTCCCCGGCTCCTCACCCTGGT +GTCAGCTGGAAGCCACCATCTCCTAAACCCACCTGtgttcttccacctct +gccagggctgccctctcctccaccttcacaaactcaattcctacccattc +tcaggtcccttatcaaatgccatctcctccatgatgcctccctgattccc +cTGCTGGAaataatggtgataacagctaaggcattggggttggctacgtg +ccaggcaaggagttggcactttacatgctttatctcatttcagccacata +acatcgacaggtggcattatgattcatatcatccccatctgatagccagg +aaaactgagtcccagagaggttagccactttcctagggccCTGTGCTCTG +ACTCAAGCATAGCTCTGAGGAACTCTAGCATTCATCAGTTTAAGCACCAT +GACTTTCTTTGCTGAGTCACCCAAGGCATTTCTTCATTTAAATGTTCTTC +CTTGGCCAGGCGCAGTGGCTCAggcccaatgcggtggctcacgcctgtaa +tctcaacactttgggaggccgaggtgggcagataatctgaggtcaggagt +tcaagaccagcctggccaacatggtgaaaccccatctctactaaaaatac +aaaaaaatgaggctgggcgtgatgactcacacctgtaatcccagcacttt +gggaggccaaggcaggtggattacatgaggtcaggagttcgagaccagcc +tggccaacatggtgaaatcctatctctattaaaaatacaaaaaattagcc +aggcatggtggcaggcacctgtaatcccagctacttgggaggctgaggca +ggaaaatggcttgaacccgggaggtggaggttgcagtgagccaaggttgc +accattgcactccagcctgggcaaaaagagggaaacatcgtctaaaaaag +aaaaaaaaaaaattagccaggctgggtggtgcatgcccgtaattccagct +actcaggaggatgaagcaagagaattgcttgaacccaggaggcagagatt +acagtgagctgagatcacaacactgcactccagcctaggtaaagaacaag +actccatctcaaaaataaataaataaaaataaaTGTTCTTCCTTGCAATG +AAGTTAAATATGTAAATTCTCAAACCAGTTGCTTAAGGGCACAGTTTTGT +TCTTTACCTATATTTTTAACAAATATTTTATGTAAGTAGTTGACAAAATC +AAATACTGTGTACACTACCGAGGCTTCCCTGGGAAAGCCATCAGCCTCTG +CCCCATCCCTTCCCACTCCTGATTCCACTTTCCTGTGTTTCCATATCTTT +TTCATGTCTGTTTCTGGCCCACAGTGGGCGATCAATACATGTTAGCCACC +AACCATCAAACCTATATTGAGTAATTATGGTATGTCAGGCACTATGCTCA +ATGAAATTGTAttaggcttgtacaaaagtaattgtggtttttaagagtaa +tggcaaaaacggcagttactttcgcaccaacTATTTGCTGCCTTGAATTA +TTCCTCCTCTCCTCATCCCTAAACCCTGCTCCTCCCAGCCATTCTTCCTC +CCCTTCTTGGGCCATGGCCAGGCCCCACCCAGGTACTAAGACTCAGGTGA +ACCAAGGAAGACTTAATGCCCACTCTTTTCTGATGCCCATGTTGGCATGT +GTTAAGtcggttagcattaagtttggctgcatttagcagagacccaaaag +aacagtgccttttaaaaggcagaggttatgtctctcacacacacccagca +caagtccaagaccagcatggcatctcagctccatcaacctcaggaaccga +gctcctgcagctccctgccctgcagttgataaggtgaggtctttgtcctc +ctggttcaagatggtgctagaatgttggctaccatatctatagtccaggc +atcagaatggagcaagggatgaaaaaggaagagatgaaggcacacgacag +gttcctgagagctggcacaggacacttctgcttatatttcactggccaga +acttagtcacatggtcacacctagttgggagactctgagaagtaaagtat +ttattctagatggccatatccctacctaagacttggagttttctatgact +ggggaagaacggaagacaagatattgggaaagactagcagcctctactaA +AAGGGTGATCtgtgttgatgtgcgtgtgtgtgtgatgtttgtatgagcat +gtgtgttatgtgttgtgtgtTGGTGGGGCAGATTCTTGCGAGCACTTTGG +TCTCAGATGGACCTGCTACCAGTTCTCTCTGCAGACCCCCATAGGTTTCT +CCTAAACCTGGCCTCTCCTATTAGGCAGCCTTACTCAGCGGCAGCTTCTC +AGCTCCATGTTTTCAAGGAACCACAATTTATTTCCAGCATCCACTGAAGC +ATATTATCAGTGGTGATAGAGGGGGCTTGTAAAACTGTTTTTCCACTTAG +GTATTAGAGGGTGGCCATTATTTGAGAGTGACTATGACCACAGTTAATCT +GGTAATAAATTCTCTTGGGTAGGAGGGGGAAAGGAAAGGATGCTTTAAGG +AAGCATCTTGCCAGGAGACACAAAGCTAACAAGAGTGGAGCCTGCAGCTG +GAGCCGCAGAGCCTAATCACTACACCCGCCCATCTCTGCTAGGGTTTCAT +GACTTCGTATCGGGGATTAGCAGTATTTAACTCTGTTGCACAAACATTTG +GTGTATTATTCAGGTAACAAGTAGCTAATAGAGGAAGTTTTACTTTTTTA +AGACATAAATTTGCCTTTTCCCAAATTACTTGGTACATAGTACTTTTCAT +GTTTGAAGTTGAGATGTGGGTACAATACCATAGCTTTATTCCAGAGCAGG +GTATTTGTTTCCAAATGCCATGTTCCCAGCAGCTGCCCTTGACTGGGAAT +TGGGGTGTGATTTGGGCTTTTCCTTAAATCCTTGAGGAGCTGGAGGGGTG +GGTGGCTCGCACTCCTGCTTTctggatctgaatcctgactctgtcatgga +cctgtttgactttgggcaagttgactcctattcctgagccccatattttt +ctcttctgtgaaattcagattaaaaAAACATGGCTTTGATCAAACATTAT +AAATAATATATAGACAGACTGCTTGTTTTTATTGTATTGCCAGAAATGAA +TCCTACTAATATTGCCATCTATGGACAGAAAATGTATTACCTGTCTTCAT +CAAGACCCAGACGAGGAAGAACACGAAAAGCGGAGATTAATTTTACTGCC +ATCTCCAGAACCGTCATCCTAATATTTACTTACATTTTATTATTATTTCA +GGCTCATGCACATATACTTAGCATGGATCATTGGCCACAGACTCGCATAC +ATTTAACTTTATTACCTTTTGCCTCATGTATCTCATTAAAATTTTGCTGC +TTAATCAAGGATCTGCATATTATTTTAATTTTAGAATTCACAGTTCCAAG +ACTTTGAAAGTTTCAAGCGTTCTGGGTGaatgtgttatgctctctcccgc +caccatgtctttataccccctgatttctcagccactatggcaaccacttt +ctactcttagtagcccatatttagtccaatccccagctcaggagacactt +cttccagggagccccctgtgccttccagtagtatcttgtacctgcccttt +ttgcaaagctctttcctcctggcttagaatggcccattgacctgtttgtt +tctcctattaaactgtaagccactcgagggtagagagcatctgttgttca +ccattgcatcctcggtgctgagcactgcgtctgacatattatttagaagg +tcagtaagtgctagtgggatTCAGGCTCCCAGTGGGTGGGAGAGAAAGGA +CGTAAGGAAGCAAGTGGTAAAGGCCCTCACAGAGTATCAGCAGGCTGGTG +TGAGGGAGAAATGCAGAGGATGGGTGAGTAGCATAATCGCTAATGATAGG +GTAATGATAGAGCACATTTCACAACACCTTtaagccctttcacgtgcatc +agataatttgatcctcataaaagcctagagatagatatattacagggatg +aaggtggagtattttgtggttatgtgatatgtttaaaattatgcagtgag +taaatgactgggttcaaaccagaccttaaaagtctgttatctttccCTCG +AGCATGCAATGAAGTCTACATCATCCCTACCATGTCCATTTGATCACACC +CTGGCCTCACAGCTCTGTGGTCTACAGGATACCTCATGGTGGTTTTATTG +ACCAGACAATAATCCTCTTTCTAAGGGGATGCATTTCATTAATACATATG +TAGATCATGAATTGTCTTTGACTTTGAGGGGATGGTAGCCAGAGCAGAAA +GCAAAGCTGATTTTCATCCCCGTCTGGTAATGTGGTTGGTAATGTGAAGA +TGGGTGTATTCTGAGATACCGGCTCCTTGCAGTGTGTGGTTCCTTCTGTT +TTCAGGCCCAAGAAGCCCATCCTGGGAAGGAAAATGCATTGGGGAACCCT +GTGCGGATTCTTGTGGCTTTGGCCCTATCTTTTCTATGTCCAAGCTGTGC +CCATCCAAAAAGTCCAAGATGACACCAAAACCCTCATCAAGACAATTGTC +ACCAGGATCAATGACATTTCACACACGGTAAGGAGAGTATGCGGGGACAA +AGTAGAACTGCAGCCAGCCCAGCACTGGCTCCTAGTGGCACTGGACCCAG +ATAGtccaagaaacatttattgaacgcctcctgaatgccaggcacctact +ggaagctgagaaGGATTTGAAAGCACAGGGCTCCACTCTTTCTGGTTGTT +TCTTTTGGCCCCTCTGCCTGCTGAGATTCCAGGGGTTAGTGGTTCTAATT +CTAAACCACTCCAAGAACATTTGATTTTGCTACATGTTTCCATTTAAAAA +TCATAGGATTTGggctgggtgtggtggcttgtacctgtcatcccagcact +ttgggaggccaaagcaggaggatcattcgagcccaagagttcgagaccag +cctgggcagcatagggagaccccatctctacaaaaataataaaaaatgtt +agctgggcatggtggtgtgtacctgtggtcccagctaggggaggctgaga +tggaaggatcacctgagcctgggaggttgaggctgcagtgggccctgatc +atgccaccgtgctccagcctgggtgacagagtgagaccttgtctcaaaat +aaataaataaataaataaaAGTCATAGGATTTgatcaggcatgatgggtc +acatctgtaagcccattgctttaggaggccaaggtaggaggatcagttga +ggccaggagttcaagaccagcctgggcaacatggcaagacctctctctct +aatttttaaaaaaataaaaaTTAAAGATAAGAAAAAAATCATAGGATTCT +CATGAGGCCTCACGTGCTTATTTTCAACCTACCAAGGGGAAACCCAGGCC +TCAGCGATTAGCTGAGCCACATGCAGGCACAGCCACTGTCTCTTTCCTTC +CTGTCCCCTCTGTCCCCACCTTCTGCGCTCGCCTTCCTCCCTGACTTCAC +TTCCTTGAATCTTAGTGCCTACGACCAGAGGGAGCTGTGAAGTTCCTTGT +GTCCCATTGGCAGGAACAAGACCCCCAGAAGCATCTCCTCAGGGCCTCTA +TCCCATCTCTAGATGTGCTTGTCATTAGGGttcttgtagttccagctgat +ctctggccctgccgctcaaagatacccaaaagagcgagtctacccttttt +cacattcaaccctctactgatttgcaaatagcagtcagtgcccaccctgg +tcttttctctggggtccagcaggcctagaccttcagccattttcctgatg +aGGTCTGTAtttgaaattaggaagattaagtttgaatcttcacacttctg +atgtctgtgagatcttcagcaagttccttactgtctttaagccttgtttt +catcatctggataatggggatatcacacactattcacaaggttgttatga +ggcctaaattagctaaagcaATTGAATCCTCCTTACCCCCTGCATGGAGC +TCTCTGGAGACTTCCACGTCTCCTGGTCATTGTGGGTGTCTTATGGTAGT +CTTGGGCAGTTAGGGAGAAGTTAGGTGTCTGGAAGCAAAGATGGCTCAGA +ACTAGATAGAGTCTTGGGCATTTTATAGATAAAAACTCTTGTCTCCttta +aaaataataaaaaaaaattaGCTGGGCATATTAGCCACTCAGCAAGACTG +CACGTGATAGATCCCGAGTGCCCCACCTTGGGTGGTGTAATACACAATAT +CACGGGAGCCCCGGGTAGTAACCACGGAGGTGTCAGCCTCAGTGCTGTGG +GCAGATGGATGGGGAGAGCCTCCCGGAACTGGAGTCACTGGAGCAGGGTT +GGGGGGCCTCACTGAGGGTACGGCCTTGATCTCTAAGGAGGAGGGACTGC +CTGGAAAAGCTGACTGGGAGGGAGGACTCGGCTGGGGGTAGAAGGGACTA +GGGAAGGCTGGGGGTGGGGGTGCTTATGGAGGACCTCAGATGCCTGGGGA +ACAGACTCCACTAAATAAAACATATGAAACCATGGCTGGTTCTTCAGCAG +AGGCCATGTAGAGAAAGGAATGACCTAGGAAAGTTGGCCTGGAAGTGGAG +GGAAGGATGGTGTGGGAAAAGCAGGAATCTCGGAGACCAGCTTAGAGGCT +TGGCAGTCACCTGGGTGCAGGATACAAGGGCCTGAGCCAAAGTGGTGAGG +GAGGGTGGAAGGAGGCAGCCCAGAGAATGACCCTCCATGCCCACGGGGAA +GGCAGAGGGCTCTGAGAGCGATTCCTCCCACATGCTGAGCACTTGTTCTC +CCTCTTCCTCCTGCATAGCAGTCAGTCTCCTCCAAACAGAAAGTCACCGG +TTTGGACTTCATTCCTGGGCTCCACCCCATCCTGACCTTATCCAAGATGG +ACCAGACACTGGCAGTCTACCAACAGATCCTCACCAGTATGCCTTCCAGA +AACGTGATCCAAATATCCAACGACCTGGAGAACCTCCGGGATCTTCTTCA +CGTGCTGGCCTTCTCTAAGAGCTGCCACTTGCCCTGGGCCAGTGGCCTGG +AGACCTTGGACAGCCTGGGGGGTGTCCTGGAAGCTTCAGGCTACTCCACA +GAGGTGGTGGCCCTGAGCAGGCTGCAGGGGTCTCTGCAGGACATGCTGTG +GCAGCTGGACCTCAGCCCTGGGTGCTGAGGCCTTGAAGGTCACTCTTCCT +GCAAGGACTACGTTAAGGGAAGGAACTCTGGCTTCCAGGTATCTCCAGGA +TTGAAGAGCATTGCATGGACACCCCTTATCCAGGACTCTGTCAATTTCCC +TGACTCCTCTAAGCCACTCTTCCAAAGGCATAAGACCCTAAGCCTCCTTT +TGCTTGAAACCAAAGATATATACACAGGATCCTATTCTCACCAGGAAGGG +GGTCCACCCAGCAAAGAGTGGGCTGCATCTGGGATTCCCACCAAGGTCTT +CAGCCATCAACAAGAGTTGTCTTGTCCCCTCTTGACCCATCTCCCCCTCA +CTGAATGCCTCAATGTGACCAGGGGTGATTTCAGAGAGGGCAGAGGGGTA +GGCAGAGCCTTTGGATGACCAGAACAAGGTTCCCTCTGAGAATTCCAAGG +AGTTCCATGAAGACCACATCCACACACGCAGGAACTCCCAGCAACACAAG +CTGGAAGCACATGTTTATTTATTCTGCATTTTATTCTGGATGGATTTGAA +GCAAAGCACCAGCTTCTCCAGGCTCTTTGGGGTCAGCCAGGGCCAGGGGT +CTCCCTGGAGTGCAGTTTCCAATCCCATAGATGGGTCTGGCTGAGCTGAA +CCCATTTTGAGTGACTCGAGGGTTGGGTTCATCTGAGCAAGAGCTGGCAA +AGGTGGCTCTCCAGTTAGTTCTCTCGTAACTGGTTTCATTTCTACTGTGA +CTGATGTTACATCACAGTGTTTGCAATGGTGTTGCCCTGAGTGGATCTCC +AAGGACCAGGTTATTTTAAAAAGATTTGTTTTGTCAAGTGTCATATGTAG +GTGTCTGCACCCAGGGGTGGGGAATGTTTGGGCAGAAGGGAGAAGGATCT +AGAATGTGTTTTCTGAATAACATTTGTGTGGTGGGTTCTTTGGAAGGAGT +GAGATCATTTTCTTATCTTCTGCAATTGCTTAGGATGTTTTTCATGAAAA +TAGCTCTTTCAGGGGGGTTGTGAGGCCTGGCCAGGCACCCCCTGGAGAGA +AGTTTCTGGCCCTGGCTGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTG +CTTCAAATCCATCCAGAATAAAACGCAAAGGGCTGAAAGCCATTTGTTGG +GGCAGTGGTAAGCTCTGGCTTTCTCCGACTGCTAGGGAGTGGTCTTTCCT +ATCATGGAGTGACGGTCCCACACTGGTGACTGCGATCTTCAGAGCAGGGG +TCCTTGGTGTGACCCTCTGAATGGTCCAGGGTTGATCACACTCTGGGTTT +ATTACATGGCAGTGTTCCTATTTGGGGCTTGCATGCCAAATTGTAGTTCT +TGTCTGATTGGCTCACCCAAGCAAGGCCAAAATTACCAAAAATCTTGGGG +GGTTTTTACTCCAGTGGTGAAGAAAACTCCTTTAGCAGGTGGTCCTGAGA +CCTGACAAGCACTGCTAGGCGAGTGCCAGGACTCCCCAGGCCAGGCCACC +AGGATGGCCCTTCCCACTGGAGGTCACATTCAGGAAGATGAAAGAGGAGG +TTTGGGGTCTGCCACCATCCTGCTGCTGTGTTTTTGCTATCACACAGTGG +GTGGTGGATCTGTCCAAGGAAACTTGAATCAAAGCAGTTAACTTTAAGac +tgagcacctgcttcatgctcagccctgactggtgctataggctggagaag +ctcacccaataaacattaagattgaggcctgccctcagggatcttgcatt +cccagtggTCAAACCGCACTCACCCATGTGCCAAGGTGGGGTATTTACCA +CAGCAGCTGAACAGCCAAATGCATGGTGCAGTTGACAGCAGGTGGGAAAT +GGTATGAGCTGAGGGGGGCCGTGCCCAGGGGCCCACAGGGAACCCTGCTT +GCACTTTGTAACATGTTTACTTTTCagggcatcttagcttctattatagc +cacatccctttgaaacaagataactgagaatttaaaaataagaaaataTG +ACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAA +TAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAAT +CCATCCAGAATAAAACGCAGATGCTTTGCTTCAAATCCATCCAGAATAAA +ACGCAAATGACCCCAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATC +CATCCAGAATAAAACGCATGACCCCAAAGAGCCTGGAGAAGCTGATGCTT +TGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAA +GCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGATGCTTTGCT +TCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCTGGAGAAGCTG +ATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCCCAAAGAGCCT +GGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAGACCC +CAAAGAGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAA +ACGCAGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCA + +#--------------------------------------- +#---------------------------------------
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/emboss_needle_out.score Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,4 @@ +Sequence hg17 20196 (604.5) + +#--------------------------------------- +#---------------------------------------
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/emboss_needleall_input1.fa Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,62 @@ +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGAC +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGA +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGACATG +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGANN +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAGTCCGACATG +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGACGGCATACGA +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGTCCGACGATC +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/emboss_needleall_input2.fq Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,100 @@ +@FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGATGTTTA ++FC12044_91407_8_200_406_24 +SXXTXXXXXXXXXTTSUXSSXKTMQ +@FC12044_91407_8_200_720_610 +CTCTGTGGCACCCCATCCCTCACTT ++FC12044_91407_8_200_720_610 +OXXXXXXXXXXXXXXXXXTSXQTXU +@FC12044_91407_8_200_345_133 +GATTTTTTAACAATAAACGTACATA ++FC12044_91407_8_200_345_133 +OQTOOSFORTFFFIIOFFFFFFFFF +@FC12044_91407_8_200_106_131 +GTTGCCCAGGCTCGTCTTGAACTCC ++FC12044_91407_8_200_106_131 +XXXXXXXXXXXXXXSXXXXISTXQS +@FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG ++FC12044_91407_8_200_916_471 +XXXXXXXXXXXXXXXUXXUSXXTXW +@FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG ++FC12044_91407_8_200_57_85 +XFXMXSXXSXXXOSQROOSROFQIQ +@FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG ++FC12044_91407_8_200_10_437 +USXSXXXXXXUXXXSXQXXUQXXKS +@FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGAACGACT ++FC12044_91407_8_200_154_436 +MKKMQTSRXMSQTOMRFOOIFFFFF +@FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGAGGGCTGCCG ++FC12044_91407_8_200_336_64 +STQMOSXSXSQXQXXKXXXKFXFFK +@FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC ++FC12044_91407_8_200_620_233 +XXXXXXXXXXXXXXXXXXXXXSXSW +@FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAGGCCCGCC ++FC12044_91407_8_200_902_349 +XMXSSXMXXSXQSXTSQXFKSKTOF +@FC12044_91407_8_200_40_618 +CTGTCCCCACGGCGGGGGGGCCTGG ++FC12044_91407_8_200_40_618 +TXXXXSXXXXXXXXXXXXXRKFOXS +@FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG ++FC12044_91407_8_200_83_511 +SOXXXXXUXXXXXXQKQKKROOQSU +@FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT ++FC12044_91407_8_200_76_246 +XTXTUXXXXXRXXXTXXSUXSRFXQ +@FC12044_91407_8_200_303_427 +TTGCGACAGAGTTTTGCTCTTGTCC ++FC12044_91407_8_200_303_427 +XXQROXXXXIXFQXXXOIQSSXUFF +@FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC ++FC12044_91407_8_200_31_299 +XRXTSXXXRXXSXQQOXQTSQSXKQ +@FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG ++FC12044_91407_8_200_553_135 +XSQQXXXXXXXXXXSXXMFFQXTKU +@FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAGCGATTATCCT ++FC12044_91407_8_200_139_74 +RMXUSXTXXQXXQUXXXSQISISSO +@FC12044_91407_8_200_108_33 +GTCATGGCGGCCCGCGCGGGGAGCG ++FC12044_91407_8_200_108_33 +OOOSSXXSXXOMKMOFMKFOKFFFF +@FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG ++FC12044_91407_8_200_980_965 +TOSSRXXXSSMSXMOMXIRXOXFFS +@FC12044_91407_8_200_981_857 +AACGAGGGGCGCGACTTGACCTTGG ++FC12044_91407_8_200_981_857 +RXMSSXXXXSXQXQXFSXQFQKMXS +@FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGCCTTGGAC ++FC12044_91407_8_200_8_865 +XXXFKOROMKOORMIMRIIKKORFF +@FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCACTCC ++FC12044_91407_8_200_292_484 +XQXOSXXXXXUXXXXIXXXXQTOXF +@FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGGGGGGTT ++FC12044_91407_8_200_675_16 +OXTXXXSXXQXXOXXKMXXMXOKQF +@FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAGCTCCCCT ++FC12044_91407_8_200_285_136 +OSXOQXXXXXSXXUXXTXXXXTRMS
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/emboss_needleall_out.fasta Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,3232 @@ +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAG--AGCTCGTATGCCGTCTT---CTGCTTG +>FC12044_91407_8_200_406_24 +--------GTTAGCTC-----CCACCTTAAGATGTTTA +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTT---TCCCTACACGACGCTCTTCCGATCT--- +>FC12044_91407_8_200_406_24 +-----GTTAGCTCCC-AC--------CTTAAGATGTTTA +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACC--GAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_406_24 +---GTTA--GCTCCCACCTTAAGATGTTTA------------------------------ +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACG--AGCT-----CTTCCGATCT--- +>FC12044_91407_8_200_406_24 +-------------------GTTAGCTCCCACCTTAAGATGTTTA +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTT---TCCCTACACGACGCTCTTCCGATCT--- +>FC12044_91407_8_200_406_24 +-----GTTAGCTCCC-AC--------CTTAAGATGTTTA +>Illumina_Paired_End_DNA_Adapters1_1 +-----------------GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGAT----------GTTTA-------------- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTT---TCCCTACACGACGCTCTTCCGATCT--- +>FC12044_91407_8_200_406_24 +-----GTTAGCTCCC-AC--------CTTAAGATGTTTA +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACC--GAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_406_24 +---GTTA--GCTCCCACCTTAAGATGTTTA------------------------------ +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGT---CTCGGCATTCCTGCTGAACCGCTCTTCCG +ATCT--- +>FC12044_91407_8_200_406_24 +--------------------------GTTAGCTC---------------CCAC-CTTAAG +ATGTTTA +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTT---TCCCTACACGACGCTCTTCCGATCT--- +>FC12044_91407_8_200_406_24 +-----GTTAGCTCCC-AC--------CTTAAGATGTTTA +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGT---CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT--- +>FC12044_91407_8_200_406_24 +--GTTAGCTC---------------CCAC-CTTAAGATGTTTA +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC------------ +>FC12044_91407_8_200_406_24 +----GT-------TAG--CTCCCACCTTAAGATGTTTA +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-TCCGAC------------- +>FC12044_91407_8_200_406_24 +-----------GTT----AGCTCCCACCTTAAGATGTTTA +>Illumina_DpnII_Gex_Adapters2_1 +--------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA--- +>Illumina_DpnII_Gex_Adapters2_2 +------TCGTATGCCGTCTT---CTGCTTG +>FC12044_91407_8_200_406_24 +GTTAGCTC-----CCACCTTAAGATGTTTA +>Illumina_DpnII_Gex_PCR_Primer_1 +--------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA--- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA +>FC12044_91407_8_200_406_24 +---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-TCCGAC-----GATC---- +>FC12044_91407_8_200_406_24 +-------------GTT----AGCTCCCACCTTAAGATGTTTA +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTG-TAGAACTCTGAAC------------ +>FC12044_91407_8_200_406_24 +-------GTTAG--CTCCCACCTTAAGATGTTTA +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-TCCGAC------ATG---- +>FC12044_91407_8_200_406_24 +-----------GTT----AGCTCCCACCTTAAGATGTTTA +>Illumina_NlaIII_Gex_Adapters2_1 +--------CAAGCAGAAGACGGCATACGANN +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA----- +>Illumina_NlaIII_Gex_Adapters2_2 +------TCGTATGCCGTCTT---CTGCTTG +>FC12044_91407_8_200_406_24 +GTTAGCTC-----CCACCTTAAGATGTTTA +>Illumina_NlaIII_Gex_PCR_Primer_1 +--------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA--- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA +>FC12044_91407_8_200_406_24 +---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- +>Illumina_NlaIII_Gex_sequencing_primer +-------CCGACAGGTTCAGA-GTTCTACAGTCCGACATG +>FC12044_91407_8_200_406_24 +GTTAGCTCC--CACCTTAAGATGTT-TA------------ +>Illumina_Small_RNA_RT_Primer +--------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA--- +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAG-TCCGAC-----GATC---- +>FC12044_91407_8_200_406_24 +-------GTT----AGCTCCCACCTTAAGATGTTTA +>Illumina_Small_RNA_3p_Adapter +------TCGTATGCCGTCTTCTGCTTGT--- +>FC12044_91407_8_200_406_24 +GTTAGCTC-----CCACCTTAAG-ATGTTTA +>Illumina_Small_RNA_PCR_Primer_1 +--------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_406_24 +GTTAGCTCCCACCTTAAGA-TGTTTA--- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA +>FC12044_91407_8_200_406_24 +---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-TCCGAC-----GATC---- +>FC12044_91407_8_200_406_24 +-------------GTT----AGCTCCCACCTTAAGATGTTTA +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATG-----CCGT------CTTCTGCTTG +>FC12044_91407_8_200_720_610 +-----------CTC-TGTGGCACCCCATCCCTCACTT------- +>Illumina_Genomic_DNA_Adapters1_2 +-------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_720_610 +CTCTGTGGCACCCCATCCCT--------CACTT------- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCT---ACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_720_610 +---------------------CTCTGTGGCACCCCATCCCT--------CACTT------ +- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCT------TCCGATCT------- +>FC12044_91407_8_200_720_610 +----------------------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_Genomic_DNA_sequencing_primer +-------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_720_610 +CTCTGTGGCACCCCATCCCT--------CACTT------- +>Illumina_Paired_End_DNA_Adapters1_2 +-------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_720_610 +CTCTGTGGCACCCCATCCCT--------CACTT------- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCT---ACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_720_610 +---------------------CTCTGTGGCACCCCATCCCT--------CACTT------ +- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTC---GGCATTCCTGCTGAACCGCTCTTCCG +ATCT +>FC12044_91407_8_200_720_610 +----------------------------CTCTGTGGCACCCCATC----CCTCACTT--- +---- +>Illumina_Paired_End_DNA_sequencing_primer_1 +-------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_720_610 +CTCTGTGGCACCCCATCCCT--------CACTT------- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTC---GGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_720_610 +----CTCTGTGGCACCCCATC----CCTCACTT------- +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCT---GAAC-------------- +>FC12044_91407_8_200_720_610 +------------------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGAC-------------- +>FC12044_91407_8_200_720_610 +--------------CT-CTGTGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGAC-----GGCATACGA---------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG------------------ +>FC12044_91407_8_200_720_610 +------------CTCTG--TGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGAC-----GGCATACGA---------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGT-----CCGA---------- +>FC12044_91407_8_200_720_610 +---------------------------------CT-CTGTGGCACCCCATCCCTCACTT +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAGT----CCGACGATC-------- +>FC12044_91407_8_200_720_610 +----------------CT-CTGTGGCACC--CCATCCCTCACTT +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCT---GAAC-------------- +>FC12044_91407_8_200_720_610 +-------------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGT-----CCGACATG-------- +>FC12044_91407_8_200_720_610 +--------------CT-CTGTGGCACCC--CATCCCTCACTT +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGAC-----GGCATACGANN-------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG------------------ +>FC12044_91407_8_200_720_610 +------------CTCTG--TGGCACCCCATCCCTCACTT +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGAC-----GGCATACGA---------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGT-----CCGA---------- +>FC12044_91407_8_200_720_610 +---------------------------------CT-CTGTGGCACCCCATCCCTCACTT +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAGT-----CCGACATG-------- +>FC12044_91407_8_200_720_610 +-----------------CT-CTGTGGCACCC--CATCCCTCACTT +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGAC-----GGCATACGA---------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGT----CCGACGATC-------- +>FC12044_91407_8_200_720_610 +----------CT-CTGTGGCACC--CCATCCCTCACTT +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCT-TGT------------------- +>FC12044_91407_8_200_720_610 +-----------------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGAC-----GGCATACGA---------- +>FC12044_91407_8_200_720_610 +-----------CTCTGTGGCACCCCATCCCTCACTT +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGT-----CCGA---------- +>FC12044_91407_8_200_720_610 +---------------------------------CT-CTGTGGCACCCCATCCCTCACTT +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAGT----CCGACGATC-------- +>FC12044_91407_8_200_720_610 +----------------CT-CTGTGGCACC--CCATCCCTCACTT +>Illumina_Genomici_DNA_Adapters1_1 +GAT--------------CGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_345_133 +GATTTTTTAACAATAAACGTA------CATA---------------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT-------------------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_Genomic_DNA_PCR_Primers1_1 +-----------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC +TTCCGATCT +>FC12044_91407_8_200_345_133 +GATTTTTTAACAAT-AAACG---------------TACA---------TA---------- +--------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +----------CAAGCAGAAGACG-GCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA---------------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTTCCGATCT-------------------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG---------------------- +>FC12044_91407_8_200_345_133 +-----------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT-------------------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_Paired_End_DNA_PCR_Primers1_1 +-----------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC +TTCCGATCT +>FC12044_91407_8_200_345_133 +GATTTTTTAACAAT-AAACG---------------TACA---------TA---------- +--------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +----------CAAGCAGAAGACG-GCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG +CTCTTCCGATCT +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA------------------------------- +------------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT-------------------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-------------------- +>FC12044_91407_8_200_345_133 +--------------------------------GATTTTTTAACAATAAACGTACATA +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC------- +>FC12044_91407_8_200_345_133 +--------GATTTTTTAACAATAAACGTACATA +>Illumina_DpnII_Gex_Adapters1_2 +--------------ACAGGTTCAGAGTTCTACAGTCCGAC +>FC12044_91407_8_200_345_133 +GATTTTTTAACAATAAACGTACATA--------------- +>Illumina_DpnII_Gex_Adapters2_1 +----------CAAGCAGAAGACG-GCATACGA +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA--- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG-------------------- +>FC12044_91407_8_200_345_133 +----------------GATTTTTTAACAATAAACGTACATA +>Illumina_DpnII_Gex_PCR_Primer_1 +----------CAAGCAGAAGACG-GCATACGA +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA--- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCT--ACAGTCCGA---------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAAT---AAACGTACATA +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCT--ACAGTCCGACGATC--- +>FC12044_91407_8_200_345_133 +-----------GATTTTTTAACAAT-AAACGTACATA +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC------- +>FC12044_91407_8_200_345_133 +---GATTTTTTAACAATAAACGTACATA +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCT--ACAGT---CCGACATG +>FC12044_91407_8_200_345_133 +---------GATTTTTTAACAATAAACGTACATA +>Illumina_NlaIII_Gex_Adapters2_1 +----------CAAGCAGAAGACG-GCATACGANN +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA----- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG-------------------- +>FC12044_91407_8_200_345_133 +----------------GATTTTTTAACAATAAACGTACATA +>Illumina_NlaIII_Gex_PCR_Primer_1 +----------CAAGCAGAAGACG-GCATACGA +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA--- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCT--ACAGTCCGA---------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAAT---AAACGTACATA +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCT--ACAGT---CCGACATG +>FC12044_91407_8_200_345_133 +------------GATTTTTTAACAATAAACGTACATA +>Illumina_Small_RNA_RT_Primer +----------CAAGCAGAAGACG-GCATACGA +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA--- +>Illumina_Small_RNA_5p_Adapter +------------------GTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_345_133 +GATTTTTTAACAATAAACGTACATA------------------- +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT------------------- +>FC12044_91407_8_200_345_133 +----------------GATTTTTTAACAATAAACGTACATA +>Illumina_Small_RNA_PCR_Primer_1 +----------CAAGCAGAAGACG-GCATACGA +>FC12044_91407_8_200_345_133 +GATTTTTTAACAA----TAAACGTACATA--- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCT--ACAGTCCGA---------- +>FC12044_91407_8_200_345_133 +----------------------------GATTTTTTAACAAT---AAACGTACATA +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCT--ACAGTCCGACGATC--- +>FC12044_91407_8_200_345_133 +-----------GATTTTTTAACAAT-AAACGTACATA +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGC-------CGTCTT---CTGCTTG +>FC12044_91407_8_200_106_131 +--------------GT-TGCCCAGGCTCGTCTTGAACTCC--- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTC-CGATCT-- +>FC12044_91407_8_200_106_131 +-----GTTGCCC------AGGCTCGTCTTGAACTCC +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC-CGATCT- +- +>FC12044_91407_8_200_106_131 +------------------------------GTTGCCC------AGGCTCGTCTTGAACTC +C +>Illumina_Genomic_DNA_PCR_Primers1_2 +-----CAAGCAGAAGACGGCATACGAGCTCTTC-CGATCT-- +>FC12044_91407_8_200_106_131 +GTTGCCCAG-----------------GCTCGTCTTGAACTCC +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTTC-CGATCT-- +>FC12044_91407_8_200_106_131 +-----GTTGCCC------AGGCTCGTCTTGAACTCC +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG---------------- +>FC12044_91407_8_200_106_131 +-------------GT----------TGCCCAGGCTCGTCTTGAACTCC +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTC-CGATCT-- +>FC12044_91407_8_200_106_131 +-----GTTGCCC------AGGCTCGTCTTGAACTCC +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC-CGATCT- +- +>FC12044_91407_8_200_106_131 +------------------------------GTTGCCC------AGGCTCGTCTTGAACTC +C +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGT---CTCGGCATTCCTGCTGAACCGCTCTTCCG +ATCT +>FC12044_91407_8_200_106_131 +--------------------------GTTGCCCAGGC--TCGTCTTGAAC------TCC- +---- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTTC-CGATCT-- +>FC12044_91407_8_200_106_131 +-----GTTGCCC------AGGCTCGTCTTGAACTCC +>Illumina_Paired_End_DNA_sequencing_primer_2 +------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_106_131 +GTTGCCCAGGCTCG-------TCTTGAAC------TCC----- +>Illumina_DpnII_Gex_Adapters1_1 +---------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_106_131 +GTTGCCCAGGCTCGTC------TTGAACTCC---- +>Illumina_DpnII_Gex_Adapters1_2 +-----ACAGGTTCAGAGTTCTACAGTCCGAC +>FC12044_91407_8_200_106_131 +GTTGCCCAGGCTC---GTCTTGAACTCC--- +>Illumina_DpnII_Gex_Adapters2_1 +------CAAGCAGAAGACGGCATACGA----- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGC-------CGTCTT---CTGCTTG +>FC12044_91407_8_200_106_131 +--GT-TGCCCAGGCTCGTCTTGAACTCC--- +>Illumina_DpnII_Gex_PCR_Primer_1 +------CAAGCAGAAGACGGCATACGA----- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT---CAGAGT--TCT---ACAGTCCGA +>FC12044_91407_8_200_106_131 +-----------------------GTTGCCCAGGCTCGTCTTGAAC--TCC-- +>Illumina_DpnII_Gex_sequencing_primer +-----CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_106_131 +GTTGCC--CAGGCTC---GTCTTGAACTCC------- +>Illumina_NlaIII_Gex_Adapters1_1 +-----------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_106_131 +GTTGCCCAGGCTCG---TCTTGAACTCC---- +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTC-CGACATG------ +>FC12044_91407_8_200_106_131 +-----------GTTGCCCAGGCTCGTCTTGAACTCC +>Illumina_NlaIII_Gex_Adapters2_1 +------CAAGCAGAAGACGGCATACGANN--- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGC-------CGTCTT---CTGCTTG +>FC12044_91407_8_200_106_131 +--GT-TGCCCAGGCTCGTCTTGAACTCC--- +>Illumina_NlaIII_Gex_PCR_Primer_1 +------CAAGCAGAAGACGGCATACGA----- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT---CAGAGT--TCT---ACAGTCCGA +>FC12044_91407_8_200_106_131 +-----------------------GTTGCCCAGGCTCGTCTTGAAC--TCC-- +>Illumina_NlaIII_Gex_sequencing_primer +----CCGACAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_106_131 +GTTGCC--CAGGCTC---GTCTTGAACTCC------ +>Illumina_Small_RNA_RT_Primer +------CAAGCAGAAGACGGCATACGA----- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_Small_RNA_5p_Adapter +GTT---CAGAGT--TCT---ACAGTCCGACGATC +>FC12044_91407_8_200_106_131 +GTTGCCCAGGCTCGTCTTGAAC--TCC------- +>Illumina_Small_RNA_3p_Adapter +TCGTATGC-------CGTCTT---CTGCTTGT +>FC12044_91407_8_200_106_131 +--GT-TGCCCAGGCTCGTCTTGAACTCC---- +>Illumina_Small_RNA_PCR_Primer_1 +------CAAGCAGAAGACGGCATACGA----- +>FC12044_91407_8_200_106_131 +GTTGCCCAGGC-----TCGTCTT--GAACTCC +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT---CAGAGT--TCT---ACAGTCCGA +>FC12044_91407_8_200_106_131 +-----------------------GTTGCCCAGGCTCGTCTTGAAC--TCC-- +>Illumina_Small_RNA_sequencing_primer +-----CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_106_131 +GTTGCC--CAGGCTC---GTCTTGAACTCC------- +>Illumina_Genomici_DNA_Adapters1_1 +-GATCGGAAGAGCTCGTATGCCGT---CTTCTGCTTG +>FC12044_91407_8_200_916_471 +TGAT-TGAAG-----GTAGG--GTAGCATACTG---- +>Illumina_Genomic_DNA_Adapters1_2 +-------------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG--------------------------- +>Illumina_Genomic_DNA_PCR_Primers1_1 +------AATG---------ATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC +GCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG----------------------------------- +------------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +-----CAAGCAGAAGACG---GCATAC-GAGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG-------------- +>Illumina_Genomic_DNA_sequencing_primer +-------------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG--------------------------- +>Illumina_Paired_End_DNA_Adapters1_1 +-GATCGGAAG--AGCGGTTCAGCAGGAATGCCGAG +>FC12044_91407_8_200_916_471 +TGAT-TGAAGGTAG-GGT--AGCA-TACTG----- +>Illumina_Paired_End_DNA_Adapters1_2 +-------------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG--------------------------- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +------AATG---------ATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC +GCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG----------------------------------- +------------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +-----CAAGCAGAAGACG---GCATAC-GAGATCGGTCTCGGCATTCCTGCTGAACCGCT +CTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG------------------------------- +---------- +>Illumina_Paired_End_DNA_sequencing_primer_1 +-------------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGTAGCATACTG--------------------------- +>Illumina_Paired_End_DNA_sequencing_primer_2 +------------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGGGT---AGCA-TACTG-------------------- +>Illumina_DpnII_Gex_Adapters1_1 +-----GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGG---GTAGCATACTG--- +>Illumina_DpnII_Gex_Adapters1_2 +------ACAGGTTCAGAGT--TCTACAGTCCGAC +>FC12044_91407_8_200_916_471 +TGATTGA-AGGT--AGGGTAGCATACTG------ +>Illumina_DpnII_Gex_Adapters2_1 +-----CAAGCAGAAGACG---GCATAC-GA +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG------------------- +>FC12044_91407_8_200_916_471 +---------------TGATTGAAGGTAGGGTAGCATACTG +>Illumina_DpnII_Gex_PCR_Primer_1 +-----CAAGCAGAAGACG---GCATAC-GA +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGT--TCTACAGTCCGA +>FC12044_91407_8_200_916_471 +--TGAT----TGA--------AGGT--AGGGTAGCATACTG----- +>Illumina_DpnII_Gex_sequencing_primer +----CGACAGGTTCAGAGT--TCTACAGTCCGACGATC +>FC12044_91407_8_200_916_471 +TGATTGA-AGGT--AGGGTAGCATACTG---------- +>Illumina_NlaIII_Gex_Adapters1_1 +----------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_916_471 +TGATTGAAGGTAGG---GTAGCATACTG--- +>Illumina_NlaIII_Gex_Adapters1_2 +------ACAGGTTCAGAGT--TCTACAGTCCGACATG +>FC12044_91407_8_200_916_471 +TGATTGA-AGGT--AGGGTAGCATACTG--------- +>Illumina_NlaIII_Gex_Adapters2_1 +-----CAAGCAGAAGACG---GCATAC-GANN +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG--- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG------------------- +>FC12044_91407_8_200_916_471 +---------------TGATTGAAGGTAGGGTAGCATACTG +>Illumina_NlaIII_Gex_PCR_Primer_1 +-----CAAGCAGAAGACG---GCATAC-GA +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGT--TCTACAGTCCGA +>FC12044_91407_8_200_916_471 +--TGAT----TGA--------AGGT--AGGGTAGCATACTG----- +>Illumina_NlaIII_Gex_sequencing_primer +---CCGACAGGTTCAGAGT--TCTACAGTCCGACATG +>FC12044_91407_8_200_916_471 +TGATTGA-AGGT--AGGGTAGCATACTG--------- +>Illumina_Small_RNA_RT_Primer +-----CAAGCAGAAGACG---GCATAC-GA +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG- +>Illumina_Small_RNA_5p_Adapter +---------GTTCAGAGT--TCTACAGTCCGACGATC +>FC12044_91407_8_200_916_471 +TGATTGAAGGT--AGGGTAGCATACTG---------- +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT------------------ +>FC12044_91407_8_200_916_471 +---------------TGATTGAAGGTAGGGTAGCATACTG +>Illumina_Small_RNA_PCR_Primer_1 +-----CAAGCAGAAGACG---GCATAC-GA +>FC12044_91407_8_200_916_471 +TGATTGAAG--GTAG--GGTAGCATACTG- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGT--TCTACAGTCCGA +>FC12044_91407_8_200_916_471 +--TGAT----TGA--------AGGT--AGGGTAGCATACTG----- +>Illumina_Small_RNA_sequencing_primer +----CGACAGGTTCAGAGT--TCTACAGTCCGACGATC +>FC12044_91407_8_200_916_471 +TGATTGA-AGGT--AGGGTAGCATACTG---------- +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCGTCTTCTG------CTTG +>FC12044_91407_8_200_57_85 +----------GCTCCAATAGCG----CAGAGGAAACCTG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTC---TTCCG--------ATCT- +>FC12044_91407_8_200_57_85 +--------------------GCTCCAATAGCGCAGAGGAAACCTG +>Illumina_Genomic_DNA_PCR_Primers1_1 +-----AATGATACGGCGACCACCGAG---ATCTACACTCTTTCCCTACACGACGCTCTTC +CGATCT +>FC12044_91407_8_200_57_85 +GCTCCAAT---------AGCGCAGAGGAAACCTG-------------------------- +------ +>Illumina_Genomic_DNA_PCR_Primers1_2 +----CAA----GCAGAAGA---CGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG-------------------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTC---TTCCG--------ATCT- +>FC12044_91407_8_200_57_85 +--------------------GCTCCAATAGCGCAGAGGAAACCTG +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAA---TGCCGAG +>FC12044_91407_8_200_57_85 +GCTC-CAATAGCG---CAG-AGGAAACCTG----- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTC---TTCCG--------ATCT- +>FC12044_91407_8_200_57_85 +--------------------GCTCCAATAGCGCAGAGGAAACCTG +>Illumina_Paired_End_DNA_PCR_Primers1_1 +-----AATGATACGGCGACCACCGAG---ATCTACACTCTTTCCCTACACGACGCTCTTC +CGATCT +>FC12044_91407_8_200_57_85 +GCTCCAAT---------AGCGCAGAGGAAACCTG-------------------------- +------ +>Illumina_Paired_End_DNA_PCR_Primers1_2 +----CAA----GCAGAAGA---CGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG +CTCTTCCGATCT +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG----------------------------------- +------------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTC---TTCCG--------ATCT- +>FC12044_91407_8_200_57_85 +--------------------GCTCCAATAGCGCAGAGGAAACCTG +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATT-------------CCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_57_85 +---GCTC--CAATAGCGCAGAGGAAACCTG-------------------- +>Illumina_DpnII_Gex_Adapters1_1 +--------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGA-----GGA------AAC-CTG--- +>Illumina_DpnII_Gex_Adapters1_2 +------ACAG-GTTCAGAGTTCTACAGTCCGAC----- +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_DpnII_Gex_Adapters2_1 +----CAA----GCAGAAGA---CGGCATACGA +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG------- +>Illumina_DpnII_Gex_Adapters2_2 +--TCGTATGCCGTCTTCTG------CTTG +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG----CAGAGGAAACCTG +>Illumina_DpnII_Gex_PCR_Primer_1 +----CAA----GCAGAAGA---CGGCATACGA +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAG-GTTCAGAGTTCTACAGTCCGA------ +>FC12044_91407_8_200_57_85 +-------------GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_DpnII_Gex_sequencing_primer +----------CGACAGGTTCAGAGTTCTACAGTCCGACGATC--- +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG-------CAGAG-----------GA--AACCTG +>Illumina_NlaIII_Gex_Adapters1_1 +-------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG--- +>Illumina_NlaIII_Gex_Adapters1_2 +------ACAG-GTTCAGAGTTCTACAGTCCG--ACATG +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_NlaIII_Gex_Adapters2_1 +----CAA----GCAGAAGA---CGGCATACGANN +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG--------- +>Illumina_NlaIII_Gex_Adapters2_2 +--TCGTATGCCGTCTTCTG------CTTG +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG----CAGAGGAAACCTG +>Illumina_NlaIII_Gex_PCR_Primer_1 +----CAA----GCAGAAGA---CGGCATACGA +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAG-GTTCAGAGTTCTACAGTCCGA------ +>FC12044_91407_8_200_57_85 +-------------GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_NlaIII_Gex_sequencing_primer +---CCGACAG-GTTCAGAGTTCTACAGTCCG--ACATG +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_Small_RNA_RT_Primer +----CAA----GCAGAAGA---CGGCATACGA +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG------- +>Illumina_Small_RNA_5p_Adapter +---------GTTCAGAGTTCTACAGTCCGACGATC--- +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAG-----------GA--AACCTG +>Illumina_Small_RNA_3p_Adapter +--TCGTATGCCGTCTTCTG------CTTGT +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG----CAGAGGAAACCTG- +>Illumina_Small_RNA_PCR_Primer_1 +----CAA----GCAGAAGA---CGGCATACGA +>FC12044_91407_8_200_57_85 +GCTCCAATAGCGCAGAGGAAACCTG------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAG-GTTCAGAGTTCTACAGTCCGA------ +>FC12044_91407_8_200_57_85 +-------------GCTCCAATAGCG--CAGAG-----------GAAACCTG +>Illumina_Small_RNA_sequencing_primer +----------CGACAGGTTCAGAGTTCTACAGTCCGACGATC--- +>FC12044_91407_8_200_57_85 +GCTCCAATAGCG-------CAGAG-----------GA--AACCTG +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_10_437 +-------------------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- +>FC12044_91407_8_200_10_437 +--------------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT +---------- +>FC12044_91407_8_200_10_437 +---------------------------------------------GCTGCTTGGGAGGCT +GAGGCAGGAG +>Illumina_Genomic_DNA_PCR_Primers1_2 +---------------CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG------------------------ +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- +>FC12044_91407_8_200_10_437 +--------------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Paired_End_DNA_Adapters1_1 +---GATCGGAAGAGCGGTTCA-GCAGGAATGCCGAG +>FC12044_91407_8_200_10_437 +GCTGCTTGG----GAGGCTGAGGCAGGAG------- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- +>FC12044_91407_8_200_10_437 +--------------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT +---------- +>FC12044_91407_8_200_10_437 +---------------------------------------------GCTGCTTGGGAGGCT +GAGGCAGGAG +>Illumina_Paired_End_DNA_PCR_Primers1_2 +---------------CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGA +ACCGCTCTTCCGATCT +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------------------------------- +---------------- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- +>FC12044_91407_8_200_10_437 +--------------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGA-TCT---------- +>FC12044_91407_8_200_10_437 +----------------GCTG-------CTTGGGAGGCTGAGGCAGGAG +>Illumina_DpnII_Gex_Adapters1_1 +---------GATCGTCGGA--CTGTAGAACTCTGAAC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGA--GGCTGAGGCAGGAG---------- +>Illumina_DpnII_Gex_Adapters1_2 +------------------ACAGGTTCAGAGTTCTACAGTCCGAC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGG---AG---------------- +>Illumina_DpnII_Gex_Adapters2_1 +---------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_10_437 +-------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_DpnII_Gex_PCR_Primer_1 +---------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_10_437 +---GCTGCTTGGGAGGCTGA-----GGCAGG---AG--------------- +>Illumina_DpnII_Gex_sequencing_primer +----------------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGG---AG-------------------- +>Illumina_NlaIII_Gex_Adapters1_1 +------TCGGA--------CTGTAGAACTCTGAAC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG---------- +>Illumina_NlaIII_Gex_Adapters1_2 +------------------ACAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGG---AG------------------- +>Illumina_NlaIII_Gex_Adapters2_1 +---------------CAAGCAGAAGACGGCATACGANN +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG------------- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_10_437 +-------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_NlaIII_Gex_PCR_Primer_1 +---------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_10_437 +---GCTGCTTGGGAGGCTGA-----GGCAGG---AG--------------- +>Illumina_NlaIII_Gex_sequencing_primer +-------------CCGA--CAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGG---AG------------------- +>Illumina_Small_RNA_RT_Primer +---------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------- +>Illumina_Small_RNA_5p_Adapter +------------GTTCA-----GAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG------------------ +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT---------------- +>FC12044_91407_8_200_10_437 +-------------GCTGCTTGGGAGGCTGAGGCAGGAG +>Illumina_Small_RNA_PCR_Primer_1 +---------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGGAG----------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_10_437 +---GCTGCTTGGGAGGCTGA-----GGCAGG---AG--------------- +>Illumina_Small_RNA_sequencing_primer +----------------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_10_437 +GCTGCTTGGGAGGCTGAGGCAGG---AG-------------------- +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGC-TCGTATGC------CGTCTTCTGCTTG +>FC12044_91407_8_200_154_436 +-------AGACCTTTGGATACAATGAACGACT-------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTAC-----ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +AGAC-CTTTGGATACAATGAACGACT------------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTAC-----ACGACGCTCTTCCGA +TCT +>FC12044_91407_8_200_154_436 +-------------------------AGAC-CTTTGGATACAATGAACGACT--------- +--- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGAC----GGCATAC---GAGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGA------ACGA-CT +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTAC-----ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +AGAC-CTTTGGATACAATGAACGACT------------ +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGC----GGTTCAGCAGGAATGCCGAG- +>FC12044_91407_8_200_154_436 +-------AGACCTTTGGATACA--ATGAA---CGACT +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTAC-----ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +AGAC-CTTTGGATACAATGAACGACT------------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTAC-----ACGACGCTCTTCCGA +TCT +>FC12044_91407_8_200_154_436 +-------------------------AGAC-CTTTGGATACAATGAACGACT--------- +--- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGAC----GGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCC +GATCT +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAA---------------------TGAACGACT----- +----- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTAC-----ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +AGAC-CTTTGGATACAATGAACGACT------------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTG------CTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_154_436 +------AGACCTT--TGGATACAATGAACGACT---------- +>Illumina_DpnII_Gex_Adapters1_1 +-GATCGTCGGACTGTAGAACTCTGAAC---- +>FC12044_91407_8_200_154_436 +AGACCTTTGGA---TACAA---TGAACGACT +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACA--GTCCGAC- +>FC12044_91407_8_200_154_436 +AGACCTTTGGA----TACAATGAACGACT +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGAC----GGCATACGA--------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_DpnII_Gex_Adapters2_2 +------TCGTATGC------CGTCTTCTGCTTG +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGAACGACT-------- +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGAC----GGCATACGA--------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_DpnII_Gex_PCR_Primer_2 +--------------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGA-ACGACT-------------------------------- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACA--GTCCGACGATC +>FC12044_91407_8_200_154_436 +--AGACCTTTGGA----TACAATGAACGACT--- +>Illumina_NlaIII_Gex_Adapters1_1 +------TCGGACTGTAGAACTCTGAAC---- +>FC12044_91407_8_200_154_436 +AGACCTTTGGA---TACAA---TGAACGACT +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACA--GTCCGACATG +>FC12044_91407_8_200_154_436 +AGACCTTTGGA----TACAATGAACGACT-- +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGAC----GGCATACGANN------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_NlaIII_Gex_Adapters2_2 +------TCGTATGC------CGTCTTCTGCTTG +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGAACGACT-------- +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGAC----GGCATACGA--------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_NlaIII_Gex_PCR_Primer_2 +--------------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGA-ACGACT-------------------------------- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACA--GTCCGACATG +>FC12044_91407_8_200_154_436 +---AGACCTTTGGA----TACAATGAACGACT-- +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGAC----GGCATACGA--------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTT----CTACA--GTCCGACGATC +>FC12044_91407_8_200_154_436 +----AGACCTTTGGATACAATGAACGACT--- +>Illumina_Small_RNA_3p_Adapter +------TCGTATGC------CGTCTTCTGCTTGT +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGAACGACT--------- +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGAC----GGCATACGA--------- +>FC12044_91407_8_200_154_436 +--------AGACCTTTGG-ATACAATGAACGACT +>Illumina_Small_RNA_PCR_Primer_2 +--------------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_154_436 +AGACCTTTGGATACAATGA-ACGACT-------------------------------- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACA--GTCCGACGATC +>FC12044_91407_8_200_154_436 +--AGACCTTTGGA----TACAATGAACGACT--- +>Illumina_Genomici_DNA_Adapters1_1 +-----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGA---GGAGGGC-----TGCCG----------- +>Illumina_Genomic_DNA_Adapters1_2 +-----ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAG---ATCTACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_336_64 +-------------------AGGGAATTT------------TAGAGGA-GGGCTGCCG--- +- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCAT-----ACGAGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +----------AGGGAATTTTAGAGGAGGGCTGCCG---- +>Illumina_Genomic_DNA_sequencing_primer +-----ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGA---ATGCCGAG +>FC12044_91407_8_200_336_64 +-------AGGGAATTTTAG-AGGAGGGCTGCCG-- +>Illumina_Paired_End_DNA_Adapters1_2 +-----ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAG---ATCTACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_336_64 +-------------------AGGGAATTT------------TAGAGGA-GGGCTGCCG--- +- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCAT-----ACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTC +CGATCT +>FC12044_91407_8_200_336_64 +----------AGGGAATTTTAGAGGAG---GG-----------CTGCCG----------- +------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +-----ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCT---------GCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_336_64 +-----AGGGAATT--TTAGAGGAGGGCTG--CCG------------ +>Illumina_DpnII_Gex_Adapters1_1 +--------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGA-----GGGCTGCCG---------- +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-------TCCGAC +>FC12044_91407_8_200_336_64 +--AGG----GAATTTTAGAGGAGGGCTGCCG-- +>Illumina_DpnII_Gex_Adapters2_1 +CAAG-------CAGAAGACGGCATACGA +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG- +>Illumina_DpnII_Gex_Adapters2_2 +------TCGTA---------TGCCGTCTTCTGCTTG +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGAGGGCTGCCG----------- +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAG-------CAGAAGACGGCATACGA +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA--------- +>FC12044_91407_8_200_336_64 +---------------------AGG----GAATTTTAGAG---GAGGGCTGCCG +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-------TCCGACGATC +>FC12044_91407_8_200_336_64 +----AGG----GAATTTTAGAGGAGGGCTGCCG------ +>Illumina_NlaIII_Gex_Adapters1_1 +--------------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGAGGGCTGCCG---------- +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-------TCCGACATG +>FC12044_91407_8_200_336_64 +--AGG----GAATTTTAGAGGAGGGCTGCCG----- +>Illumina_NlaIII_Gex_Adapters2_1 +CAAG-------CAGAAGACGGCATACGANN +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG--- +>Illumina_NlaIII_Gex_Adapters2_2 +------TCGTA---------TGCCGTCTTCTGCTTG +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGAGGGCTGCCG----------- +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAG-------CAGAAGACGGCATACGA +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA--------- +>FC12044_91407_8_200_336_64 +---------------------AGG----GAATTTTAGAG---GAGGGCTGCCG +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAG-------TCCGACATG +>FC12044_91407_8_200_336_64 +-----AGG----GAATTTTAGAGGAGGGCTGCCG----- +>Illumina_Small_RNA_RT_Primer +CAAG-------CAGAAGACGGCATACGA +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG- +>Illumina_Small_RNA_5p_Adapter +GTTCA--GAGTTCTACAG-------TCCGACGATC +>FC12044_91407_8_200_336_64 +----AGGGAATTTTAGAGGAGGGCTGCCG------ +>Illumina_Small_RNA_3p_Adapter +------TCGTA---------TGCCGTCTTCTGCTTGT +>FC12044_91407_8_200_336_64 +AGGGAATTTTAGAGGAGGGCTGCCG------------ +>Illumina_Small_RNA_PCR_Primer_1 +CAAG-------CAGAAGACGGCATACGA +>FC12044_91407_8_200_336_64 +--AGGGAATTTTAGAGGAGGGCTGCCG- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA--------- +>FC12044_91407_8_200_336_64 +---------------------AGG----GAATTTTAGAG---GAGGGCTGCCG +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-------TCCGACGATC +>FC12044_91407_8_200_336_64 +----AGG----GAATTTTAGAGGAGGGCTGCCG------ +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_620_233 +-------------------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACG------ACGCTCTTCCGATCT- +>FC12044_91407_8_200_620_233 +----------TCTCCATGTTGGTCAGGCT-----GGTCTC +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTT--TCCCTACACGACGCTCTTCCGATCT +- +>FC12044_91407_8_200_620_233 +----------------------TCTCCA-TGTTGGTC--------AGGCT-----GGTCT +C +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT---------------- +>FC12044_91407_8_200_620_233 +-----------------------TCT--CCATGTTGGTCAGGCTGGTCTC +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACG------ACGCTCTTCCGATCT- +>FC12044_91407_8_200_620_233 +----------TCTCCATGTTGGTCAGGCT-----GGTCTC +>Illumina_Paired_End_DNA_Adapters1_1 +----------GATCGGAAGAGC-GGT-TCAGCAGGAATGCCGAG +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAG----GCTGGTCTC--------------- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACG------ACGCTCTTCCGATCT- +>FC12044_91407_8_200_620_233 +----------TCTCCATGTTGGTCAGGCT-----GGTCTC +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTT--TCCCTACACGACGCTCTTCCGATCT +- +>FC12044_91407_8_200_620_233 +----------------------TCTCCA-TGTTGGTC--------AGGCT-----GGTCT +C +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCAT-----TCCTGCTGAACCG-CTCTT +CCGATCT +>FC12044_91407_8_200_620_233 +---------------------------TCTC--CATGTTGGTCAGGCTG----GTCTC-- +------- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACG------ACGCTCTTCCGATCT- +>FC12044_91407_8_200_620_233 +----------TCTCCATGTTGGTCAGGCT-----GGTCTC +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCAT-----TCCTGCTGAACCG-CTCTTCCGATCT +>FC12044_91407_8_200_620_233 +---TCTC--CATGTTGGTCAGGCTG----GTCTC--------- +>Illumina_DpnII_Gex_Adapters1_1 +-------GATCGTCGGACT-GTAGAACTCTGAAC +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGT----CTC----- +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACA-----GTCCGAC------- +>FC12044_91407_8_200_620_233 +-------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_DpnII_Gex_Adapters2_1 +-------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC--------- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_620_233 +-------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_DpnII_Gex_PCR_Primer_1 +-------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC--------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA-----GTCCGA-------- +>FC12044_91407_8_200_620_233 +--------------------------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACA-----GTCCGAC-GATC-- +>FC12044_91407_8_200_620_233 +---------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_NlaIII_Gex_Adapters1_1 +------------TCGGACT-GTAGAACTCTGAAC +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGT----CTC----- +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACA-----GTCCGACATG----- +>FC12044_91407_8_200_620_233 +-------------TCTCCATGTTGGTCAGGC-TGGTCTC +>Illumina_NlaIII_Gex_Adapters2_1 +-------------CAAGCAGAAGACGGCATACGANN +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC----------- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_620_233 +-------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_NlaIII_Gex_PCR_Primer_1 +-------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC--------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA-----GTCCGA-------- +>FC12044_91407_8_200_620_233 +--------------------------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACA-----GTCCGACATG----- +>FC12044_91407_8_200_620_233 +----------------TCTCCATGTTGGTCAGGC-TGGTCTC +>Illumina_Small_RNA_RT_Primer +-------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC--------- +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACA-----GTCCGAC-GATC-- +>FC12044_91407_8_200_620_233 +---------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT---------------- +>FC12044_91407_8_200_620_233 +-------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_Small_RNA_PCR_Primer_1 +-------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_620_233 +TCTCCATGTTGGTCAGGCTGGTCTC--------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA-----GTCCGA-------- +>FC12044_91407_8_200_620_233 +--------------------------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACA-----GTCCGAC-GATC-- +>FC12044_91407_8_200_620_233 +---------------TCTCCATGTTGGTCAGGCTGGTCTC +>Illumina_Genomici_DNA_Adapters1_1 +----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_902_349 +TGAACGTCGAGA-CGCAAG-GCCC----GCC------------ +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACAC-----GACGC----TCTTCCGATCT +>FC12044_91407_8_200_902_349 +------------TGAACGTCGAGACGCAAGGCCCGCC----- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGA----------CCACCGAGATCTACACTCTTTCCCTACACGACGCTCT +TCCGATCT +>FC12044_91407_8_200_902_349 +--TGA-ACGTCGAGACGCAAGGCCCGCC-------------------------------- +-------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGA------AGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_902_349 +-----TGAACGTCGAGACG-----CAAGGCCCGCC----- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACAC-----GACGC----TCTTCCGATCT +>FC12044_91407_8_200_902_349 +------------TGAACGTCGAGACGCAAGGCCCGCC----- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-------------- +>FC12044_91407_8_200_902_349 +---------------------TGAACGTCGAGACGCAAGGCCCGCC +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACAC-----GACGC----TCTTCCGATCT +>FC12044_91407_8_200_902_349 +------------TGAACGTCGAGACGCAAGGCCCGCC----- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGA----------CCACCGAGATCTACACTCTTTCCCTACACGACGCTCT +TCCGATCT +>FC12044_91407_8_200_902_349 +--TGA-ACGTCGAGACGCAAGGCCCGCC-------------------------------- +-------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCG---GTCTCGGCATTCCTGCTGAACCGCTCTTCCG +ATCT +>FC12044_91407_8_200_902_349 +-----TGA--ACG----TCGAGA-CGCAAGGCCCGCC----------------------- +---- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACAC-----GACGC----TCTTCCGATCT +>FC12044_91407_8_200_902_349 +------------TGAACGTCGAGACGCAAGGCCCGCC----- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAA----------------CCGCTCTTCCGATCT +>FC12044_91407_8_200_902_349 +------------------TGAACGTCGAGACGCAAGGCCCGCC---------- +>Illumina_DpnII_Gex_Adapters1_1 +-GATCGTCGGACTGTAGAACTCTGAAC------- +>FC12044_91407_8_200_902_349 +TGAACGTCG------AGA---CGCAAGGCCCGCC +>Illumina_DpnII_Gex_Adapters1_2 +---ACAGGTTCAGA-GTTCTACA--GTCCGAC +>FC12044_91407_8_200_902_349 +TGAAC--GTCGAGACG-----CAAGGCCCGCC +>Illumina_DpnII_Gex_Adapters2_1 +--------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC----- +>Illumina_DpnII_Gex_Adapters2_2 +-----------TCGTATG--CCGTCTTCTGCTTG +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAGGCCCGCC--------- +>Illumina_DpnII_Gex_PCR_Primer_1 +--------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC----- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGA----------CCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_902_349 +--TGA-ACGTCGAGACGCAAGGCCCGCC-------------------------- +>Illumina_DpnII_Gex_sequencing_primer +-------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACG--CA-AG-------GCCCGCC---- +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC-------------------- +>FC12044_91407_8_200_902_349 +----------------TGAACGTCGAGACGCAAGGCCCGCC +>Illumina_NlaIII_Gex_Adapters1_2 +---ACAGGTTCAGA-GTTCTACA--GTCCGACATG +>FC12044_91407_8_200_902_349 +TGAAC--GTCGAGACG-----CAAGGCCCGCC--- +>Illumina_NlaIII_Gex_Adapters2_1 +--------------CAAGCAGAAGACGGCATACGANN +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC------- +>Illumina_NlaIII_Gex_Adapters2_2 +-----------TCGTATG--CCGTCTTCTGCTTG +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAGGCCCGCC--------- +>Illumina_NlaIII_Gex_PCR_Primer_1 +--------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC----- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGA----------CCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_902_349 +--TGA-ACGTCGAGACGCAAGGCCCGCC-------------------------- +>Illumina_NlaIII_Gex_sequencing_primer +------CCGACAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACG--CA-AG-------GCCCGCC--- +>Illumina_Small_RNA_RT_Primer +--------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC----- +>Illumina_Small_RNA_5p_Adapter +-----GTTCAGA-GTTCTACA--GTCCGACGATC +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACG-----CAAGGCCCGCC---- +>Illumina_Small_RNA_3p_Adapter +-----------TCGTATG--CCGTCTTCTGCTTGT +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAGGCCCGCC---------- +>Illumina_Small_RNA_PCR_Primer_1 +--------------CAAGCAGAAGACGGCATACGA +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACGCAAG-----GCCCGCC----- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGA----------CCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_902_349 +--TGA-ACGTCGAGACGCAAGGCCCGCC-------------------------- +>Illumina_Small_RNA_sequencing_primer +-------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_902_349 +TGAACGTCGAGACG--CA-AG-------GCCCGCC---- +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCGTCTTC----------TGCTTG- +>FC12044_91407_8_200_40_618 +-------------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACG-------CTCTTCCGATCT +>FC12044_91407_8_200_40_618 +-----CTGTCCC--CACGGCGGGGGGGCCTGG-------- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG-------CTCTTCC +GATCT +>FC12044_91407_8_200_40_618 +------------------------------CTGTCCC--CACGGCGGGGGGGCCTGG--- +----- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT----------------------- +>FC12044_91407_8_200_40_618 +--------------------------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACG-------CTCTTCCGATCT +>FC12044_91407_8_200_40_618 +-----CTGTCCC--CACGGCGGGGGGGCCTGG-------- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGT----TCAGCAGGAATGCCGAG +>FC12044_91407_8_200_40_618 +-----------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACG-------CTCTTCCGATCT +>FC12044_91407_8_200_40_618 +-----CTGTCCC--CACGGCGGGGGGGCCTGG-------- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG-------CTCTTCC +GATCT +>FC12044_91407_8_200_40_618 +------------------------------CTGTCCC--CACGGCGGGGGGGCCTGG--- +----- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTC-----------GGCATTCCTGCTGAACCG +CTCTTCCGATCT +>FC12044_91407_8_200_40_618 +------------------------CTGTCCCCACGGCGGGGGGG----CCTGG------- +------------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACG-------CTCTTCCGATCT +>FC12044_91407_8_200_40_618 +-----CTGTCCC--CACGGCGGGGGGGCCTGG-------- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTC-----------GGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_40_618 +CTGTCCCCACGGCGGGGGGG----CCTGG------------------- +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC------------------- +>FC12044_91407_8_200_40_618 +--------------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGT-CCGAC--------------- +>FC12044_91407_8_200_40_618 +-----------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_DpnII_Gex_Adapters2_1 +-------CAAGCAGAAGACGGCATACGA---------- +>FC12044_91407_8_200_40_618 +CTGTCCCC---------ACGG----CGGGGGGGCCTGG +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTC----------TGCTTG- +>FC12044_91407_8_200_40_618 +-------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_DpnII_Gex_PCR_Primer_1 +-------CAAGCAGAAGACGGCATACGA---------- +>FC12044_91407_8_200_40_618 +CTGTCCCC---------ACGG----CGGGGGGGCCTGG +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA---------------- +- +>FC12044_91407_8_200_40_618 +------------------------------------CTGTCCCCACGGCGGGGGGGCCTG +G +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC------------------ +>FC12044_91407_8_200_40_618 +-------------------CTGTCC------CCACGGCGGGGGGGCCTGG +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC------------------- +>FC12044_91407_8_200_40_618 +---------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGACATG-------------- +>FC12044_91407_8_200_40_618 +-----------------CTGTCC-CCACGGCGGGGGGGCCTGG +>Illumina_NlaIII_Gex_Adapters2_1 +---------------------CAAGCAGAAGACGGCATACGANN +>FC12044_91407_8_200_40_618 +CTGTCCCCACGGCGGGGGGGCCTGG------------------- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTC----------TGCTTG- +>FC12044_91407_8_200_40_618 +-------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_NlaIII_Gex_PCR_Primer_1 +-------CAAGCAGAAGACGGCATACGA---------- +>FC12044_91407_8_200_40_618 +CTGTCCCC---------ACGG----CGGGGGGGCCTGG +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA---------------- +- +>FC12044_91407_8_200_40_618 +------------------------------------CTGTCCCCACGGCGGGGGGGCCTG +G +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAGTCCGACATG-------------- +>FC12044_91407_8_200_40_618 +--------------------CTGTCC-CCACGGCGGGGGGGCCTGG +>Illumina_Small_RNA_RT_Primer +-------CAAGCAGAAGACGGCATACGA---------- +>FC12044_91407_8_200_40_618 +CTGTCCCC---------ACGG----CGGGGGGGCCTGG +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGTCCGACGATC------------------ +>FC12044_91407_8_200_40_618 +-------------CTGTCC------CCACGGCGGGGGGGCCTGG +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT--------------------- +>FC12044_91407_8_200_40_618 +------------------CTGTCCCCACGGCGGGGGGGCCTGG +>Illumina_Small_RNA_PCR_Primer_1 +-------CAAGCAGAAGACGGCATACGA---------- +>FC12044_91407_8_200_40_618 +CTGTCCCC---------ACGG----CGGGGGGGCCTGG +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA---------------- +- +>FC12044_91407_8_200_40_618 +------------------------------------CTGTCCCCACGGCGGGGGGGCCTG +G +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC------------------ +>FC12044_91407_8_200_40_618 +-------------------CTGTCC------CCACGGCGGGGGGGCCTGG +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_83_511 +-----GATGTACTCTTACACCCAGACTT-TG---- +>Illumina_Genomic_DNA_Adapters1_2 +---ACACTCTTTCCCTACAC---GAC---GCTCTTCCGATCT +>FC12044_91407_8_200_83_511 +GATGTACTCT-----TACACCCAGACTTTG------------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC---GAC---GCTCTTCCG +ATCT +>FC12044_91407_8_200_83_511 +--------------------GATGTACTCT-------TACACCCAGACTTTG-------- +---- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTT-----CCGATCT--- +>FC12044_91407_8_200_83_511 +---------GATG---TA----CTCTTACACCCAGA-CTTTG +>Illumina_Genomic_DNA_sequencing_primer +---ACACTCTTTCCCTACAC---GAC---GCTCTTCCGATCT +>FC12044_91407_8_200_83_511 +GATGTACTCT-----TACACCCAGACTTTG------------ +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATG----------CCGAG------ +>FC12044_91407_8_200_83_511 +-----------------------GATGTACTCTTACACCCAGACTTTG +>Illumina_Paired_End_DNA_Adapters1_2 +---ACACTCTTTCCCTACAC---GAC---GCTCTTCCGATCT +>FC12044_91407_8_200_83_511 +GATGTACTCT-----TACACCCAGACTTTG------------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC---GAC---GCTCTTCCG +ATCT +>FC12044_91407_8_200_83_511 +--------------------GATGTACTCT-------TACACCCAGACTTTG-------- +---- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTT-----C +CGATCT--- +>FC12044_91407_8_200_83_511 +----------------------------------------GATGTA---CTCTTACACCC +AGA-CTTTG +>Illumina_Paired_End_DNA_sequencing_primer_1 +---ACACTCTTTCCCTACAC---GAC---GCTCTTCCGATCT +>FC12044_91407_8_200_83_511 +GATGTACTCT-----TACACCCAGACTTTG------------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTT-----CCGATCT--- +>FC12044_91407_8_200_83_511 +----------------GATGTA---CTCTTACACCCAGA-CTTTG +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGA-AC---------- +>FC12044_91407_8_200_83_511 +--------GA-TGT---ACTCTTACACCCAGACTTTG +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGA-GTTC---TACAGTCCGAC---- +>FC12044_91407_8_200_83_511 +---------GATGTACTCTTACACCCAGACTTTG +>Illumina_DpnII_Gex_Adapters2_1 +------------CAAGCAGA---AGACGGCATACGA +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG----------- +>Illumina_DpnII_Gex_Adapters2_2 +-------TCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTT-TG---- +>Illumina_DpnII_Gex_PCR_Primer_1 +------------CAAGCAGA---AGACGGCATACGA +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG----------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTC---TACAGTCCGA----- +>FC12044_91407_8_200_83_511 +----------------------------GATGTACTCTTACACCCAGACTTTG +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGA-GTTC---TACAGTCCGAC---GATC +>FC12044_91407_8_200_83_511 +-----------GATGTACTCTTACACCCAGACTTTG--- +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGA-AC---------- +>FC12044_91407_8_200_83_511 +---GA-TGT---ACTCTTACACCCAGACTTTG +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGA-GTTC---TACAGTCCGACATG- +>FC12044_91407_8_200_83_511 +---------GATGTACTCTTACACCCAGACTTTG +>Illumina_NlaIII_Gex_Adapters2_1 +------------CAAGCAGA---AGACGGCATACGANN +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG------------- +>Illumina_NlaIII_Gex_Adapters2_2 +-------TCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTT-TG---- +>Illumina_NlaIII_Gex_PCR_Primer_1 +------------CAAGCAGA---AGACGGCATACGA +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG----------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTC---TACAGTCCGA----- +>FC12044_91407_8_200_83_511 +----------------------------GATGTACTCTTACACCCAGACTTTG +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGA-GTTC---TACAGTCCGACATG- +>FC12044_91407_8_200_83_511 +------------GATGTACTCTTACACCCAGACTTTG +>Illumina_Small_RNA_RT_Primer +------------CAAGCAGA---AGACGGCATACGA +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG----------- +>Illumina_Small_RNA_5p_Adapter +GTTCAGA-GTTC---TACAGTCCGAC---GATC +>FC12044_91407_8_200_83_511 +-----GATGTACTCTTACACCCAGACTTTG--- +>Illumina_Small_RNA_3p_Adapter +-------TCGTATGCC--GTCTTCTGCTTGT +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTT-TG----- +>Illumina_Small_RNA_PCR_Primer_1 +------------CAAGCAGA---AGACGGCATACGA +>FC12044_91407_8_200_83_511 +GATGTACTCTTACACCCAGACTTTG----------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGA-GTTC---TACAGTCCGA----- +>FC12044_91407_8_200_83_511 +----------------------------GATGTACTCTTACACCCAGACTTTG +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGA-GTTC---TACAGTCCGAC---GATC +>FC12044_91407_8_200_83_511 +-----------GATGTACTCTTACACCCAGACTTTG--- +>Illumina_Genomici_DNA_Adapters1_1 +---------GATC--GGAAGAGCTC---GTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTG-----GCTCCCAGT----------------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTC------CGATCT----------- +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC------CG +ATCT----------- +>FC12044_91407_8_200_76_246 +--------------------------------------------------TCAAGGGTGG +ATCTTGGCTCCCAGT +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTTC------CGATCT----------- +>FC12044_91407_8_200_76_246 +--------------------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTTC------CGATCT----------- +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATG---CCGAG- +>FC12044_91407_8_200_76_246 +--TC--AAGGGTGGATC-------TTGGCTCCCAGT +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTC------CGATCT----------- +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC------CG +ATCT----------- +>FC12044_91407_8_200_76_246 +--------------------------------------------------TCAAGGGTGG +ATCTTGGCTCCCAGT +>Illumina_Paired_End_DNA_PCR_Primers1_2 +-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCC---TGCTGAACCGCTCTTCC +GATCT +>FC12044_91407_8_200_76_246 +TCAAG-----------------GGTGGATCTTGGC--TCCCAGT---------------- +----- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTTC------CGATCT----------- +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_Paired_End_DNA_sequencing_primer_2 +--------CGGTCTCGGCATTCC---TGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGC--TCCCAGT--------------------- +>Illumina_DpnII_Gex_Adapters1_1 +---------GATCGTCGGACT----GTAGAACTCTGAAC +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTT--GGCTCCCAGT------------ +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTC-AGAGT---TCT------ACAGTCCGAC +>FC12044_91407_8_200_76_246 +------TCAAGGGTGGATCTTGGCTCCCAGT----- +>Illumina_DpnII_Gex_Adapters2_1 +-CAAG----------------CAGAAGACGGCATACGA +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT------------- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTC-----TG---CTTG--------- +>FC12044_91407_8_200_76_246 +-------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_DpnII_Gex_PCR_Primer_1 +-CAAG----------------CAGAAGACGGCATACGA +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT------------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCT------ACAGTCCGA +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT---- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTC-AGAGT---TCT------ACAGTCCGACGATC +>FC12044_91407_8_200_76_246 +--------TCAAGGGTGGATCTTGGCTCCCAGT--------- +>Illumina_NlaIII_Gex_Adapters1_1 +TC--GGACTGTAGAACTCTGAAC----- +>FC12044_91407_8_200_76_246 +TCAAGG---GTGGATCTTGGCTCCCAGT +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTC-AGAGT---TCT------ACAGTCCGACATG +>FC12044_91407_8_200_76_246 +------TCAAGGGTGGATCTTGGCTCCCAGT-------- +>Illumina_NlaIII_Gex_Adapters2_1 +-CAAG----------------CAGAAGACGGCATACGANN +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT--------------- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTC-----TG---CTTG--------- +>FC12044_91407_8_200_76_246 +-------------TCAAGGGTGGATCTTGGCTCCCAGT +>Illumina_NlaIII_Gex_PCR_Primer_1 +-CAAG----------------CAGAAGACGGCATACGA +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT------------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCT------ACAGTCCGA +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT---- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTC-AGAGT---TCT------ACAGTCCGACATG +>FC12044_91407_8_200_76_246 +---------TCAAGGGTGGATCTTGGCTCCCAGT-------- +>Illumina_Small_RNA_RT_Primer +-CAAG----------------CAGAAGACGGCATACGA +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT------------- +>Illumina_Small_RNA_5p_Adapter +GTTC-AGAGT---TCT------ACAGTCCGACGATC +>FC12044_91407_8_200_76_246 +--TCAAGGGTGGATCTTGGCTCCCAGT--------- +>Illumina_Small_RNA_3p_Adapter +TC------GTATGCCGTCTT--CTGCTTGT +>FC12044_91407_8_200_76_246 +TCAAGGGTGGA-----TCTTGGCTCCCAGT +>Illumina_Small_RNA_PCR_Primer_1 +-CAAG----------------CAGAAGACGGCATACGA +>FC12044_91407_8_200_76_246 +TCAAGGGTGGATCTTGGCTCCCAGT------------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCT------ACAGTCCGA +>FC12044_91407_8_200_76_246 +-------------------------TCAAGGGTGGATCTTGGCTCCCAGT---- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTC-AGAGT---TCT------ACAGTCCGACGATC +>FC12044_91407_8_200_76_246 +--------TCAAGGGTGGATCTTGGCTCCCAGT--------- +>Illumina_Genomici_DNA_Adapters1_1 +GAT--CGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_303_427 +--TTGCGACAGAG---TTTTGC--TCTTGTCC--- +>Illumina_Genomic_DNA_Adapters1_2 +-----ACA--------CTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +TTGCGACAGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +-------TTGCGAC-----AGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGA---------GCTCT--TCCGATCT +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC----- +>Illumina_Genomic_DNA_sequencing_primer +-----ACA--------CTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +TTGCGACAGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-------------- +>FC12044_91407_8_200_303_427 +--------------TT--GC--GA---CAGAGTTTTGCTCTTGTCC +>Illumina_Paired_End_DNA_Adapters1_2 +-----ACA--------CTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +TTGCGACAGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +-------TTGCGAC-----AGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCA----TTCCTGCTGAACCGCTCTTCC +GATCT +>FC12044_91407_8_200_303_427 +---------------------------TTGCGACAGAGTTT--TGCT------CTTGTCC +----- +>Illumina_Paired_End_DNA_sequencing_primer_1 +-----ACA--------CTCTT-TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +TTGCGACAGAGTTTTGCTCTTGTCC---------------------- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCA----TTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_303_427 +---TTGCGACAGAGTTT--TGCT------CTTGTCC----- +>Illumina_DpnII_Gex_Adapters1_1 +GAT--CGTCGGACTGTAGAACTCTGAAC- +>FC12044_91407_8_200_303_427 +--TTGCGACAGAGTTTTG--CTCTTGTCC +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTT----CAGAGTT---CTACAGTCCGAC +>FC12044_91407_8_200_303_427 +-----TTGCGACAGAGTTTTGCTCTTGTCC--- +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGA------------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTC----TTCTG--CTTG--- +>FC12044_91407_8_200_303_427 +-----TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA------------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT----CAGAGTT---CTACAGTCCGA +>FC12044_91407_8_200_303_427 +------------------------TTGCGACAGAGTTTTGCTCTTGTCC-- +>Illumina_DpnII_Gex_sequencing_primer +---CGACAGGTTCAGAGTT---CTACAGTCCGACGATC +>FC12044_91407_8_200_303_427 +TTGCGA------CAGAGTTTTGCTCTTGTCC------- +>Illumina_NlaIII_Gex_Adapters1_1 +-TCGGACTGTAGAACTCTGAAC------ +>FC12044_91407_8_200_303_427 +TTGCGAC---AGAGTTTTGCTCTTGTCC +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTT----CAGAGTT---CTACAGTCCGACATG +>FC12044_91407_8_200_303_427 +-----TTGCGACAGAGTTTTGCTCTTGTCC------ +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGANN----------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTC----TTCTG--CTTG--- +>FC12044_91407_8_200_303_427 +-----TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA------------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT----CAGAGTT---CTACAGTCCGA +>FC12044_91407_8_200_303_427 +------------------------TTGCGACAGAGTTTTGCTCTTGTCC-- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTT----CAGAGTT---CTACAGTCCGACATG +>FC12044_91407_8_200_303_427 +--------TTGCGACAGAGTTTTGCTCTTGTCC------ +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGACGGCATACGA------------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_Small_RNA_5p_Adapter +GTT----CAGAGTT---CTACAGTCCGACGATC +>FC12044_91407_8_200_303_427 +-TTGCGACAGAGTTTTGCTCTTGTCC------- +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTC----TTCTG--CTTGT-- +>FC12044_91407_8_200_303_427 +-----TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA------------------- +>FC12044_91407_8_200_303_427 +---------------TTGCGACAGAGTTTTGCTCTTGTCC +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTT----CAGAGTT---CTACAGTCCGA +>FC12044_91407_8_200_303_427 +------------------------TTGCGACAGAGTTTTGCTCTTGTCC-- +>Illumina_Small_RNA_sequencing_primer +---CGACAGGTTCAGAGTT---CTACAGTCCGACGATC +>FC12044_91407_8_200_303_427 +TTGCGA------CAGAGTTTTGCTCTTGTCC------- +>Illumina_Genomici_DNA_Adapters1_1 +---GATCGGAAGAGCTC---GTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_31_299 +TCTGCTC----CAGCTCCAAG-ACGCCGCC--------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCT-TTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----TCTGCTCCAGCTCCAAGACGC-CGCC------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----------------------TCTGC------TCCAGCTCCAAGACGC-CGCC------ +>Illumina_Genomic_DNA_PCR_Primers1_2 +------CAAGC----AGAAGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------------------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCT-TTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----TCTGCTCCAGCTCCAAGACGC-CGCC------ +>Illumina_Paired_End_DNA_Adapters1_1 +---GATCGGAAGAGCGGTTCAGC----AGGAATGCCGAG +>FC12044_91407_8_200_31_299 +TCTGCTC------------CAGCTCCAAG--ACGCCGCC +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCT-TTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----TCTGCTCCAGCTCCAAGACGC-CGCC------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----------------------TCTGC------TCCAGCTCCAAGACGC-CGCC------ +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAA-----CCGCTCTTC +CGATCT +>FC12044_91407_8_200_31_299 +-----------------------------TCTGC--TCCAGCTCCAAGACGCCGCC---- +------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCT-TTCC--CTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +----TCTGCTCCAGCTCCAAGACGC-CGCC------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAA-----CCGCTCTTCCGATCT +>FC12044_91407_8_200_31_299 +-----TCTGC--TCCAGCTCCAAGACGCCGCC---------- +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC------------------ +>FC12044_91407_8_200_31_299 +-------------------TCTGCTCCAGCTCCAAGACGCCGCC +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-TCC--GAC------ +>FC12044_91407_8_200_31_299 +------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_DpnII_Gex_Adapters2_1 +------CAAGC----AGAAGACGGCATACGA +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------ +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_31_299 +-------------TCTGCTCCAGCTCCAAGACGCCGCC +>Illumina_DpnII_Gex_PCR_Primer_1 +------CAAGC----AGAAGACGGCATACGA +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------ +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA--------- +>FC12044_91407_8_200_31_299 +-------------------------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-TCC--GACGATC---- +>FC12044_91407_8_200_31_299 +--------TC----TGCTCCAGCTCCAAGACG--CCGCC +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC------------------ +>FC12044_91407_8_200_31_299 +--------------TCTGCTCCAGCTCCAAGACGCCGCC +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG-TCCGACATG----- +>FC12044_91407_8_200_31_299 +------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_NlaIII_Gex_Adapters2_1 +------CAAGC----AGAAGACGGCATACGANN +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC-------- +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG----------------- +>FC12044_91407_8_200_31_299 +-------------TCTGCTCCAGCTCCAAGACGCCGCC +>Illumina_NlaIII_Gex_PCR_Primer_1 +------CAAGC----AGAAGACGGCATACGA +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------ +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA--------- +>FC12044_91407_8_200_31_299 +-------------------------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAG-TCCGACATG----- +>FC12044_91407_8_200_31_299 +---------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_Small_RNA_RT_Primer +------CAAGC----AGAAGACGGCATACGA +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------ +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAG-TCC--GACGATC---- +>FC12044_91407_8_200_31_299 +--TC----TGCTCCAGCTCCAAGACG--CCGCC +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT------------------- +>FC12044_91407_8_200_31_299 +-------------TCTGC---TCCAGCTCCAAGACGCCGCC +>Illumina_Small_RNA_PCR_Primer_1 +------CAAGC----AGAAGACGGCATACGA +>FC12044_91407_8_200_31_299 +TCTGCTCCAGCTCCAAGACGCCGCC------ +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA--------- +>FC12044_91407_8_200_31_299 +-------------------------TC----TGCTCCAGCTCCAAGACGCCGCC +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAG-TCC--GACGATC---- +>FC12044_91407_8_200_31_299 +--------TC----TGCTCCAGCTCCAAGACG--CCGCC +>Illumina_Genomici_DNA_Adapters1_1 +----GATC--------GGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------------------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTAC----------------ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +------------TACGGAGCCGCGGGCGGGAAAGGCG------------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCAC---CGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_553_135 +-----TACGGAG-CCGCGGGCGGGA------------------AAGGCG----------- +- +>Illumina_Genomic_DNA_PCR_Primers1_2 +---CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG------------ +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTAC----------------ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +------------TACGGAGCCGCGGGCGGGAAAGGCG------------ +>Illumina_Paired_End_DNA_Adapters1_1 +GAT-CGGAAGAGCGGTTCAGCAGGAATGCCGAG +>FC12044_91407_8_200_553_135 +--TACGGAGCCGCGG----GCGGGAAAGGCG-- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTAC----------------ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +------------TACGGAGCCGCGGGCGGGAAAGGCG------------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCAC---CGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC +T +>FC12044_91407_8_200_553_135 +-----TACGGAG-CCGCGGGCGGGA------------------AAGGCG----------- +- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGC-TGAACCGCTCTTCCGAT +CT +>FC12044_91407_8_200_553_135 +----------------TACG-GA---GCCGCGG-------GCGGGAAAGGCG-------- +-- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTAC----------------ACGACGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +------------TACGGAGCCGCGGGCGGGAAAGGCG------------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +----------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGG--GCGG----------GAAAGGCG---------- +>Illumina_DpnII_Gex_Adapters1_1 +----GATCGTCGGACTGTAGAACTCTGAAC---- +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGG---------GAAAGGCG +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTAC--AGTCCGAC-------------- +>FC12044_91407_8_200_553_135 +---------------TACGGAG-CCG-CGGGCGGGAAAGGCG +>Illumina_DpnII_Gex_Adapters2_1 +----------CAAGCAG--AAGACGGCATACGA +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------- +>Illumina_DpnII_Gex_Adapters2_2 +-TCGTATGCCGTCTTCTGCTTG----------- +>FC12044_91407_8_200_553_135 +TACGGA-GCC-------GCGGGCGGGAAAGGCG +>Illumina_DpnII_Gex_PCR_Primer_1 +----------CAAGCAG--AAGACGGCATACGA +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_553_135 +-----TACGGAG-CCGCGGGCGGG-----------AAAGGCG-- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTAC--AGTCCGACGATC---------- +>FC12044_91407_8_200_553_135 +-----------------TACGGAG-CCG-CGGGCGGGAAAGGCG +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC----------------- +>FC12044_91407_8_200_553_135 +--------TA-----CGGAGCCGCGGGCGGGAAAGGCG +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTAC--AGTCC--------GACATG-- +>FC12044_91407_8_200_553_135 +---------------TACGGAG-CCGCGGGCGGGAAAGGCG +>Illumina_NlaIII_Gex_Adapters2_1 +----------CAAGCAG--AAGACGGCATACGANN +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG---------- +>Illumina_NlaIII_Gex_Adapters2_2 +-TCGTATGCCGTCTTCTGCTTG----------- +>FC12044_91407_8_200_553_135 +TACGGA-GCC-------GCGGGCGGGAAAGGCG +>Illumina_NlaIII_Gex_PCR_Primer_1 +----------CAAGCAG--AAGACGGCATACGA +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_553_135 +-----TACGGAG-CCGCGGGCGGG-----------AAAGGCG-- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTAC--AGTCC--------GACATG-- +>FC12044_91407_8_200_553_135 +------------------TACGGAG-CCGCGGGCGGGAAAGGCG +>Illumina_Small_RNA_RT_Primer +----------CAAGCAG--AAGACGGCATACGA +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------- +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTAC--AGTCCGACGATC---------- +>FC12044_91407_8_200_553_135 +-----------TACGGAG-CCG-CGGGCGGGAAAGGCG +>Illumina_Small_RNA_3p_Adapter +----------------------TCGTATGCCGTCTTCTGCTTGT +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG------------------- +>Illumina_Small_RNA_PCR_Primer_1 +----------CAAGCAG--AAGACGGCATACGA +>FC12044_91407_8_200_553_135 +TACGGAGCCGCGGGCGGGAAAGGCG-------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_553_135 +-----TACGGAG-CCGCGGGCGGG-----------AAAGGCG-- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTAC--AGTCCGACGATC---------- +>FC12044_91407_8_200_553_135 +-----------------TACGGAG-CCG-CGGGCGGGAAAGGCG +>Illumina_Genomici_DNA_Adapters1_1 +--------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTC--AAGCGAT--TATCCT------------ +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCT-ACACGACGCTCTTC---CGAT----CT +>FC12044_91407_8_200_139_74 +----------CCTCCCAGG------TTCAAGCGATTATCCT +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_139_74 +-------------CCTCCCAGGTTCAAGCGATTATCCT-------------------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTC-----TTC---CGAT----CT +>FC12044_91407_8_200_139_74 +---------------------CCTCCCAGGTTCAAGCGATTATCCT +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCT-ACACGACGCTCTTC---CGAT----CT +>FC12044_91407_8_200_139_74 +----------CCTCCCAGG------TTCAAGCGATTATCCT +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGC------GGTTCAGCAGGAATGCCGAG------ +>FC12044_91407_8_200_139_74 +-----------CCTCCCAGGTTCA--AG------CGATTATCCT +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCT-ACACGACGCTCTTC---CGAT----CT +>FC12044_91407_8_200_139_74 +----------CCTCCCAGG------TTCAAGCGATTATCCT +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_139_74 +-------------CCTCCCAGGTTCAAGCGATTATCCT-------------------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCT-----GCTGAACCGCTCTTC +CGATCT +>FC12044_91407_8_200_139_74 +-------------------------------------CCTCCCAGGTTCAAGCGATTATC +CT---- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCT-ACACGACGCTCTTC---CGAT----CT +>FC12044_91407_8_200_139_74 +----------CCTCCCAGG------TTCAAGCGATTATCCT +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCT-----GCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_139_74 +-------------CCTCCCAGGTTCAAGCGATTATCCT---- +>Illumina_DpnII_Gex_Adapters1_1 +----------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAGCGATTATC---CT-------------- +>Illumina_DpnII_Gex_Adapters1_2 +----ACAGGTTCAGAG------TTCTACAGTCCGAC +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCA-AGCGATTATCCT---------- +>Illumina_DpnII_Gex_Adapters2_1 +-----------CAAGCAGAAGACGGCATACGA------- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTC--------------TTCTGCTTG +>FC12044_91407_8_200_139_74 +--------CCTCCCAGGTTCAAGCGATTATCCT-- +>Illumina_DpnII_Gex_PCR_Primer_1 +-----------CAAGCAGAAGACGGCATACGA------- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA------- +>FC12044_91407_8_200_139_74 +-------------CCTCC--CAGGTTCA-AG----------CGATTATCCT +>Illumina_DpnII_Gex_sequencing_primer +----CGACAGGTTCAGAGTTCTACAGTCCGACGATC-- +>FC12044_91407_8_200_139_74 +CCTCC--CAGGTTCA-AG----------CGATTATCCT +>Illumina_NlaIII_Gex_Adapters1_1 +---------------------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAGCGATTATC---CT-------------- +>Illumina_NlaIII_Gex_Adapters1_2 +----ACAGGTTCAGAG------TTCTACAGTCCGACATG +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCA-AGCGATTATCCT------------- +>Illumina_NlaIII_Gex_Adapters2_1 +-----------CAAGCAGAAGACGGCATACGANN----- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTC--------------TTCTGCTTG +>FC12044_91407_8_200_139_74 +--------CCTCCCAGGTTCAAGCGATTATCCT-- +>Illumina_NlaIII_Gex_PCR_Primer_1 +-----------CAAGCAGAAGACGGCATACGA------- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA------- +>FC12044_91407_8_200_139_74 +-------------CCTCC--CAGGTTCA-AG----------CGATTATCCT +>Illumina_NlaIII_Gex_sequencing_primer +---CCGACAGGTTCAGAG------TTCTACAGTCCGACATG +>FC12044_91407_8_200_139_74 +CCTCC--CAGGTTCA-AGCGATTATCCT------------- +>Illumina_Small_RNA_RT_Primer +-----------CAAGCAGAAGACGGCATACGA------- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_Small_RNA_5p_Adapter +--------GTTCAGAGTTCTACAGTCCGACGATC-- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCA-AG----------CGATTATCCT +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTC--------------TTCTGCTTGT +>FC12044_91407_8_200_139_74 +--------CCTCCCAGGTTCAAGCGATTATCCT--- +>Illumina_Small_RNA_PCR_Primer_1 +-----------CAAGCAGAAGACGGCATACGA------- +>FC12044_91407_8_200_139_74 +CCTCCCAGGTTCAAG--------------CGATTATCCT +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA------- +>FC12044_91407_8_200_139_74 +-------------CCTCC--CAGGTTCA-AG----------CGATTATCCT +>Illumina_Small_RNA_sequencing_primer +----CGACAGGTTCAGAGTTCTACAGTCCGACGATC-- +>FC12044_91407_8_200_139_74 +CCTCC--CAGGTTCA-AG----------CGATTATCCT +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGT-ATGCCGTCTTCTGCTTG------- +>FC12044_91407_8_200_108_33 +--------------GTCATGGCGGC--CCGCGCGGGGAGCG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTT------CCGATCT------- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACC---------ACCGAGATCTACACTCTTTCCCTACACGACGCTCTT +CCGATCT +>FC12044_91407_8_200_108_33 +---GTCATGGCGGCCCGCGCGGGGAGCG-------------------------------- +------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAG--AAGACGGCATAC-----GAGCTCTTCCGATCT +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG---------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTT------CCGATCT------- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Paired_End_DNA_Adapters1_1 +-------------GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG +>FC12044_91407_8_200_108_33 +GTCATGGCGGCCCGCGCGG-GGAGCG------------------- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTT------CCGATCT------- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACC---------ACCGAGATCTACACTCTTTCCCTACACGACGCTCTT +CCGATCT +>FC12044_91407_8_200_108_33 +---GTCATGGCGGCCCGCGCGGGGAGCG-------------------------------- +------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGC---TGAACCGCTCTTCCG +ATCT +>FC12044_91407_8_200_108_33 +------------GTCAT--------GG---CGG----CCCGCGCGGGGAGCG-------- +---- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTT------CCGATCT------- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGC---TGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_108_33 +--GTCATGGC-GGCCCGCGCGGGGAGCG------------ +>Illumina_DpnII_Gex_Adapters1_1 +--------------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_108_33 +GTCATGGCGGCCCGCGCGGGGAGCG--------------------- +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTC----CGAC-------------- +>FC12044_91407_8_200_108_33 +-------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAG--AAGACGGCATACGA-------- +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_DpnII_Gex_Adapters2_2 +TCGT-ATGCCGTCTTCTGCTTG------- +>FC12044_91407_8_200_108_33 +--GTCATGGCGGC--CCGCGCGGGGAGCG +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAG--AAGACGGCATACGA-------- +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_108_33 +---GTCATGGCGGCCCGCG-CGGG----GAG----------CG- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTC-------------CGACGATC- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_NlaIII_Gex_Adapters1_1 +----------------------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_108_33 +GTCATGGCGGCCCGCGCGGGGAGCG------------------ +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGACATG------------------- +>FC12044_91407_8_200_108_33 +-----------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAG--AAGACGGCATACGANN------ +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_NlaIII_Gex_Adapters2_2 +TCGT-ATGCCGTCTTCTGCTTG------- +>FC12044_91407_8_200_108_33 +--GTCATGGCGGC--CCGCGCGGGGAGCG +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAG--AAGACGGCATACGA-------- +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_108_33 +---GTCATGGCGGCCCGCG-CGGG----GAG----------CG- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAGTCCGACATG------------------- +>FC12044_91407_8_200_108_33 +--------------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Small_RNA_RT_Primer +CAAGCAG--AAGACGGCATACGA-------- +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGTC-------------CGACGATC- +>FC12044_91407_8_200_108_33 +---------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Small_RNA_3p_Adapter +TCGT-ATGCCGTCTTCTGCTTGT------ +>FC12044_91407_8_200_108_33 +--GTCATGGCGGC--CCGCGCGGGGAGCG +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAG--AAGACGGCATACGA-------- +>FC12044_91407_8_200_108_33 +------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_108_33 +---GTCATGGCGGCCCGCG-CGGG----GAG----------CG- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTC-------------CGACGATC- +>FC12044_91407_8_200_108_33 +---------------------GTCATGGCGGCCCGCGCGGGGAGCG +>Illumina_Genomici_DNA_Adapters1_1 +-------GATC----GGAAGAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAG-TCG------------------ +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGA-----CGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTA-AAGAAGAGTCG------------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +-------------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGC +TCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG----------------------------------- +----------- +>Illumina_Genomic_DNA_PCR_Primers1_2 +------------CAAGCAGAAGA--CGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG--------------------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGA-----CGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTA-AAGAAGAGTCG------------ +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAG-CGGTTCAGCAGGAATGCCGAG--- +>FC12044_91407_8_200_980_965 +-------ACAGTGGGTTCTTAAAGAA----GAGTCG +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGA-----CGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTA-AAGAAGAGTCG------------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +-------------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGC +TCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG----------------------------------- +----------- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +------------CAAGCAGAAGA--CGGCATACGAGATCGGTCTCGGCATTCCTGCTGAA +CCGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG--------------------------------- +--------------- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGA-----CGCTCTTCCGATCT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTA-AAGAAGAGTCG------------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT------------- +>FC12044_91407_8_200_980_965 +---------------------ACAG----TGGGTTCTTAAAGAAGAGTCG +>Illumina_DpnII_Gex_Adapters1_1 +-------------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGA--GTCG------------------ +>Illumina_DpnII_Gex_Adapters1_2 +ACA---GGTTC----AGAGTTCTACAGTCCGAC +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGA-----AGAGTCG--- +>Illumina_DpnII_Gex_Adapters2_1 +------------CAAGCAGAAGA--CGGCATACGA +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG-------- +>Illumina_DpnII_Gex_Adapters2_2 +----------------------TCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG------------------ +>Illumina_DpnII_Gex_PCR_Primer_1 +------------CAAGCAGAAGA--CGGCATACGA +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG-------- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACA---GGTTC----AGAGTTCTACAGTCCGA +>FC12044_91407_8_200_980_965 +-------------------ACAGTGGGTTCTTAAAGA-----AGAGTCG-- +>Illumina_DpnII_Gex_sequencing_primer +CGACA---GGTTC----AGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_980_965 +--ACAGTGGGTTCTTAAAGA-----AGAGTCG------- +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC--------- +>FC12044_91407_8_200_980_965 +----ACAGT-GGGTTCTTAAAGAAGAGTCG +>Illumina_NlaIII_Gex_Adapters1_2 +ACA---GGTTC----AGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGA-----AGAGTCG------ +>Illumina_NlaIII_Gex_Adapters2_1 +------------CAAGCAGAAGA--CGGCATACGANN +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG---------- +>Illumina_NlaIII_Gex_Adapters2_2 +----------------------TCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG------------------ +>Illumina_NlaIII_Gex_PCR_Primer_1 +------------CAAGCAGAAGA--CGGCATACGA +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG-------- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACA---GGTTC----AGAGTTCTACAGTCCGA +>FC12044_91407_8_200_980_965 +-------------------ACAGTGGGTTCTTAAAGA-----AGAGTCG-- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACA---GGTTC----AGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_980_965 +---ACAGTGGGTTCTTAAAGA-----AGAGTCG------ +>Illumina_Small_RNA_RT_Primer +------------CAAGCAGAAGA--CGGCATACGA +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG-------- +>Illumina_Small_RNA_5p_Adapter +---GTTCAGAGTTCT------ACAGTCCGACGATC +>FC12044_91407_8_200_980_965 +ACAGT---GGGTTCTTAAAGAAGAGTCG------- +>Illumina_Small_RNA_3p_Adapter +----------------------TCGTATGCCGTCTTCTGCTTGT +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAAAGAAGAGTCG------------------- +>Illumina_Small_RNA_PCR_Primer_1 +------------CAAGCAGAAGA--CGGCATACGA +>FC12044_91407_8_200_980_965 +ACAGTGGGTTCTTAA--AGAAGAGTCG-------- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACA---GGTTC----AGAGTTCTACAGTCCGA +>FC12044_91407_8_200_980_965 +-------------------ACAGTGGGTTCTTAAAGA-----AGAGTCG-- +>Illumina_Small_RNA_sequencing_primer +CGACA---GGTTC----AGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_980_965 +--ACAGTGGGTTCTTAAAGA-----AGAGTCG------- +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGC-CGTCTTCTGCTTG- +>FC12044_91407_8_200_981_857 +-AACG--AGGG-------GCGCGACTTGACCTTGG +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +--------------AACGAGGGGCGCGACTT--GACCTTGG +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGA----CGC-TCTTCCGA +TCT--- +>FC12044_91407_8_200_981_857 +---------------------------------------AACGAGGGGCGCGACTT--GA +CCTTGG +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAA-GACGGCATACGAGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +-------AACGAGGG-----GCGCGACTT--GACCTTGG +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +--------------AACGAGGGGCGCGACTT--GACCTTGG +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG------------------- +>FC12044_91407_8_200_981_857 +-----------------------AA---CGAGGGGCGCGACTTGACCTTGG +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +--------------AACGAGGGGCGCGACTT--GACCTTGG +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGA----CGC-TCTTCCGA +TCT--- +>FC12044_91407_8_200_981_857 +---------------------------------------AACGAGGGGCGCGACTT--GA +CCTTGG +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATT--CCTGCTGAACCGCTCTTCCGA +TCT +>FC12044_91407_8_200_981_857 +----------------AACGAG---GGGCGCGAC-TTGACCT--TGG------------- +--- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +--------------AACGAGGGGCGCGACTT--GACCTTGG +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAAC------CGC-TCTTCCGATCT--- +>FC12044_91407_8_200_981_857 +--------------------AACGAGGGGCGCGACTT--GACCTTGG +>Illumina_DpnII_Gex_Adapters1_1 +---GATCGTCG-GACTGTAGAACTCTGAAC---- +>FC12044_91407_8_200_981_857 +AACGAGGGGCGCGACT---------TGACCTTGG +>Illumina_DpnII_Gex_Adapters1_2 +-AC-AGGTTC-AGAGTTCTACAGTCCGAC----- +>FC12044_91407_8_200_981_857 +AACGAGGGGCGCGACTT---------GACCTTGG +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGA-------------------- +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_DpnII_Gex_Adapters2_2 +--------TCGTATGCCGTCTTCTGCTTG- +>FC12044_91407_8_200_981_857 +AACGAGGGGCG-----CGACTTGACCTTGG +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------------- +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGAC-------AGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_981_857 +AACGA-GGGGCG-----CGACTTGACCTTGG-------------------- +>Illumina_DpnII_Gex_sequencing_primer +--CGACAGGTTCAGAGTTCTACAGTCCGACGATC---- +>FC12044_91407_8_200_981_857 +AACGAGGGGCGC-GACTT------------GACCTTGG +>Illumina_NlaIII_Gex_Adapters1_1 +--------TCG-GACTGTAGAACTCTGAAC---- +>FC12044_91407_8_200_981_857 +AACGAGGGGCGCGACT---------TGACCTTGG +>Illumina_NlaIII_Gex_Adapters1_2 +-AC-AGGTTCAGAGTTCTACAGTC-CGACATG------- +>FC12044_91407_8_200_981_857 +AACGAGG--------------GGCGCGACTTGACCTTGG +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGANN------------------ +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_NlaIII_Gex_Adapters2_2 +--------TCGTATGCCGTCTTCTGCTTG- +>FC12044_91407_8_200_981_857 +AACGAGGGGCG-----CGACTTGACCTTGG +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------------- +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGAC-------AGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_981_857 +AACGA-GGGGCG-----CGACTTGACCTTGG-------------------- +>Illumina_NlaIII_Gex_sequencing_primer +CCGAC-AGGTTCAGAGTTCTACAGTC-CGACATG------- +>FC12044_91407_8_200_981_857 +--AACGAGG--------------GGCGCGACTTGACCTTGG +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGACGGCATACGA-------------------- +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACA-------GTCCGAC--GATC---- +>FC12044_91407_8_200_981_857 +--------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_Small_RNA_3p_Adapter +--------TCGTATGCCGTCTTCTGCTTGT +>FC12044_91407_8_200_981_857 +AACGAGGGGCG-----CGACTTGACCTTGG +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------------- +>FC12044_91407_8_200_981_857 +----------------AACGAGGGGCGCGACTTGACCTTGG +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGAC-------AGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_981_857 +AACGA-GGGGCG-----CGACTTGACCTTGG-------------------- +>Illumina_Small_RNA_sequencing_primer +--CGACAGGTTCAGAGTTCTACAGTCCGACGATC---- +>FC12044_91407_8_200_981_857 +AACGAGGGGCGC-GACTT------------GACCTTGG +>Illumina_Genomici_DNA_Adapters1_1 +-------------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG--- +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGA-------AGC-----------------CTTGGAC +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTA---CACGACGCTCTT---CCGATCT +>FC12044_91407_8_200_8_865 +------TTTCCC-ACCCCAGGAAGC-CTTGGAC------ +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTA---CACGACGCTCTT---CCG +ATCT +>FC12044_91407_8_200_8_865 +-------------------------------TTTCCC-ACCCCAGGAAGC-CTTGGAC-- +---- +>Illumina_Genomic_DNA_PCR_Primers1_2 +---------CAAGCAGAAGAC--GGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC--------------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTA---CACGACGCTCTT---CCGATCT +>FC12044_91407_8_200_8_865 +------TTTCCC-ACCCCAGGAAGC-CTTGGAC------ +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTT-----CAGCAGGAATGCCGAG--- +>FC12044_91407_8_200_8_865 +--------------TTTCCCACCCCAGGAA-GCCTTGGAC +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTA---CACGACGCTCTT---CCGATCT +>FC12044_91407_8_200_8_865 +------TTTCCC-ACCCCAGGAAGC-CTTGGAC------ +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTA---CACGACGCTCTT---CCG +ATCT +>FC12044_91407_8_200_8_865 +-------------------------------TTTCCC-ACCCCAGGAAGC-CTTGGAC-- +---- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +---------CAAGCAGAAGAC--GGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG +CTCTTCCGATCT +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC------------------------------ +------------ +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTA---CACGACGCTCTT---CCGATCT +>FC12044_91407_8_200_8_865 +------TTTCCC-ACCCCAGGAAGC-CTTGGAC------ +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTC--------GGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_8_865 +---TTTCCCACCCCAGG-AAGCCT--TGGAC-------------- +>Illumina_DpnII_Gex_Adapters1_1 +--------------GATCGTCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGCCTTGGAC--------------- +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAGTCCGAC----------------- +>FC12044_91407_8_200_8_865 +------------TT------TCCCACCCCAGGAAGCCTTGGAC +>Illumina_DpnII_Gex_Adapters2_1 +---------CAAGCAGAAGAC--GGCATACGA +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC-- +>Illumina_DpnII_Gex_Adapters2_2 +--TC--------GTATGCCGTCTTCTGCTTG--- +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGC---------CTTGGAC +>Illumina_DpnII_Gex_PCR_Primer_1 +---------CAAGCAGAAGAC--GGCATACGA +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC-- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_8_865 +----TTTC-----CCACC-CCAGG--AAGCCTTGGAC------- +>Illumina_DpnII_Gex_sequencing_primer +-------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGG--AAGCCTTGGAC------------ +>Illumina_NlaIII_Gex_Adapters1_1 +-------------------TCGGACTGTAGAACTCTGAAC +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGCCTTGGAC--------------- +>Illumina_NlaIII_Gex_Adapters1_2 +---------ACAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGG--AAGCCTTGGAC----------- +>Illumina_NlaIII_Gex_Adapters2_1 +---------CAAGCAGAAGAC--GGCATACGANN +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC---- +>Illumina_NlaIII_Gex_Adapters2_2 +--TC--------GTATGCCGTCTTCTGCTTG--- +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGC---------CTTGGAC +>Illumina_NlaIII_Gex_PCR_Primer_1 +---------CAAGCAGAAGAC--GGCATACGA +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC-- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_8_865 +----TTTC-----CCACC-CCAGG--AAGCCTTGGAC------- +>Illumina_NlaIII_Gex_sequencing_primer +---CCGA---CAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGG--AAGCCTTGGAC----------- +>Illumina_Small_RNA_RT_Primer +---------CAAGCAGAAGAC--GGCATACGA +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC-- +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGTCCGACGATC---------------- +>FC12044_91407_8_200_8_865 +--------TT------TCCCAC---CCCAGGAAGCCTTGGAC +>Illumina_Small_RNA_3p_Adapter +--TC--------GTATGCCGTCTTCTGCTTGT-- +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGGAAGC---------CTTGGAC +>Illumina_Small_RNA_PCR_Primer_1 +---------CAAGCAGAAGAC--GGCATACGA +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAG--GAAGCCTTGG---AC-- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_8_865 +----TTTC-----CCACC-CCAGG--AAGCCTTGGAC------- +>Illumina_Small_RNA_sequencing_primer +-------CGACAGGTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_8_865 +TTTCCCACCCCAGG--AAGCCTTGGAC------------ +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGC--TCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_292_484 +--TC-----AGCCTCCG--TGCCCAGCCCACTCC--- +>Illumina_Genomic_DNA_Adapters1_2 +--ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +----------------------TC-AGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Genomic_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCA-CTCC +>Illumina_Genomic_DNA_sequencing_primer +--ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-------- +>FC12044_91407_8_200_292_484 +---------------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_Paired_End_DNA_Adapters1_2 +--ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +----------------------TC-AGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC +T +>FC12044_91407_8_200_292_484 +-----------------------------TCAGCCTCCGTGCCCAGCC-CACTCC----- +- +>Illumina_Paired_End_DNA_sequencing_primer_1 +--ACACTCTTTCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCAC-----TCC----- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT +>FC12044_91407_8_200_292_484 +-----TCAGCCTCCGTGCCCAGCC-CACTCC------ +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACT--GT----AG--AACTCTGAAC +>FC12044_91407_8_200_292_484 +-----TCAGCCTCCGTGCCCAGCCCACTCC---- +>Illumina_DpnII_Gex_Adapters1_2 +ACAGGTTCAG----AGTTCTACAGTCCGAC--- +>FC12044_91407_8_200_292_484 +------TCAGCCTCCGTGC-CCAG-CCCACTCC +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGA-------------- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_DpnII_Gex_Adapters2_2 +-------TCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_292_484 +TCAGCCTCCG--TGCCCAGCCCACTCC--- +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAG----AGTTCTACAG-----TCCGA +>FC12044_91407_8_200_292_484 +-------------------------TCAGCCTCCGTGC-CCAGCCCACTCC-- +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAG----AGTTCTACAGTCCGACGATC- +>FC12044_91407_8_200_292_484 +--------TCAGCCTCCGTGC-CCAG-CCCAC--TCC +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACT--GT----AG--AACTCTGAAC +>FC12044_91407_8_200_292_484 +TCAGCCTCCGTGCCCAGCCCACTCC---- +>Illumina_NlaIII_Gex_Adapters1_2 +ACAGGTTCAGAGTTCTACAG--TCCGACATG------------- +>FC12044_91407_8_200_292_484 +----------------TCAGCCTCCG---TGCCCAGCCCACTCC +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGANN------------ +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_NlaIII_Gex_Adapters2_2 +-------TCGTATGCC--GTCTTCTGCTTG +>FC12044_91407_8_200_292_484 +TCAGCCTCCG--TGCCCAGCCCACTCC--- +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAG----AGTTCTACAG-----TCCGA +>FC12044_91407_8_200_292_484 +-------------------------TCAGCCTCCGTGC-CCAGCCCACTCC-- +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAG--TCCGACATG------------- +>FC12044_91407_8_200_292_484 +-------------------TCAGCCTCCG---TGCCCAGCCCACTCC +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGACGGCATACGA-------------- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_Small_RNA_5p_Adapter +GTTCAG----AGTTCTACAGTCCGACGATC- +>FC12044_91407_8_200_292_484 +--TCAGCCTCCGTGC-CCAG-CCCAC--TCC +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTT--GT-------------- +>FC12044_91407_8_200_292_484 +-------------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA-------------- +>FC12044_91407_8_200_292_484 +----------TCAGCCTCCGTGCCCAGCCCACTCC +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAG----AGTTCTACAG-----TCCGA +>FC12044_91407_8_200_292_484 +-------------------------TCAGCCTCCGTGC-CCAGCCCACTCC-- +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAG----AGTTCTACAGTCCGACGATC- +>FC12044_91407_8_200_292_484 +--------TCAGCCTCCGTGC-CCAG-CCCAC--TCC +>Illumina_Genomici_DNA_Adapters1_1 +GATCGGAAGAGCTCGTATGCCG------TCTTCTGCTTG +>FC12044_91407_8_200_675_16 +-CTCGGGAG-GCT--GAGGCAGGGGGGTT---------- +>Illumina_Genomic_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT----------------------- +>FC12044_91407_8_200_675_16 +-------------------------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-- +--------------------- +>FC12044_91407_8_200_675_16 +--------------------------------------------------------CTCG +GGAGGCTGAGGCAGGGGGGTT +>Illumina_Genomic_DNA_PCR_Primers1_2 +-----CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---------- +>Illumina_Genomic_DNA_sequencing_primer +ACACTCTTTCCCTACACGACGCTCTTCCGATCT----------------------- +>FC12044_91407_8_200_675_16 +-------------------------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_Paired_End_DNA_Adapters1_1 +-----------GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA--GGCAGGGGGGTT---------------- +>Illumina_Paired_End_DNA_Adapters1_2 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT----------------------- +>FC12044_91407_8_200_675_16 +-------------------------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-- +--------------------- +>FC12044_91407_8_200_675_16 +--------------------------------------------------------CTCG +GGAGGCTGAGGCAGGGGGGTT +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACC------GCTCTT +CCGATCT +>FC12044_91407_8_200_675_16 +----------------------------CTCGGGA----GGCTGAGGCAGGGGGGTT--- +------- +>Illumina_Paired_End_DNA_sequencing_primer_1 +ACACTCTTTCCCTACACGACGCTCTTCCGATCT----------------------- +>FC12044_91407_8_200_675_16 +-------------------------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCCTGCTGAACC------GCTCTTCCGATCT +>FC12044_91407_8_200_675_16 +----CTCGGGA----GGCTGAGGCAGGGGGGTT---------- +>Illumina_DpnII_Gex_Adapters1_1 +GATCGTCGGACTGTAGAACTCTGAAC----------------- +>FC12044_91407_8_200_675_16 +------------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_DpnII_Gex_Adapters1_2 +--------------ACAGGTTCAGAGTTCTACAGTCCGAC +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGG---GGGGTT------------ +>Illumina_DpnII_Gex_Adapters2_1 +-----CAAGCAGAAGACGGCA-------TACGA +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---- +>Illumina_DpnII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG--------------------- +>FC12044_91407_8_200_675_16 +-----------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_DpnII_Gex_PCR_Primer_1 +-----CAAGCAGAAGACGGCA-------TACGA +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---- +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_675_16 +-------CTCGGGAGGCTGA-----GGCAGG---GGGGTT----------- +>Illumina_DpnII_Gex_sequencing_primer +--CGACAGGTTCA-------GAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGGGGGGTT---------------- +>Illumina_NlaIII_Gex_Adapters1_1 +TCGGACTGTAGAACTCTGAAC----------------- +>FC12044_91407_8_200_675_16 +-------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_NlaIII_Gex_Adapters1_2 +--------------ACAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGG---GGGGTT--------------- +>Illumina_NlaIII_Gex_Adapters2_1 +-----CAAGCAGAAGACGGCA-------TACGANN +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT------ +>Illumina_NlaIII_Gex_Adapters2_2 +TCGTATGCCGTCTTCTGCTTG--------------------- +>FC12044_91407_8_200_675_16 +-----------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_NlaIII_Gex_PCR_Primer_1 +-----CAAGCAGAAGACGGCA-------TACGA +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---- +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_675_16 +-------CTCGGGAGGCTGA-----GGCAGG---GGGGTT----------- +>Illumina_NlaIII_Gex_sequencing_primer +---------CCGA--CAGGTTCAGAGTTCTACAGTCCGACATG +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGG---GGGGTT--------------- +>Illumina_Small_RNA_RT_Primer +-----CAAGCAGAAGACGGCA-------TACGA +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---- +>Illumina_Small_RNA_5p_Adapter +----------------------GTTCAGAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGGGGGGTT----------------------- +>Illumina_Small_RNA_3p_Adapter +TCGTATGCCGTCTTCTGCTTGT-------------------- +>FC12044_91407_8_200_675_16 +-----------------CTCGGGAGGCTGAGGCAGGGGGGTT +>Illumina_Small_RNA_PCR_Primer_1 +-----CAAGCAGAAGACGGCA-------TACGA +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGA----GGCAGGGGGGTT---- +>Illumina_Small_RNA_PCR_Primer_2 +AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCGA +>FC12044_91407_8_200_675_16 +-------CTCGGGAGGCTGA-----GGCAGG---GGGGTT----------- +>Illumina_Small_RNA_sequencing_primer +--CGACAGGTTCA-------GAGTTCTACAGTCCGACGATC +>FC12044_91407_8_200_675_16 +CTCGGGAGGCTGAGGCAGGGGGGTT---------------- +>Illumina_Genomici_DNA_Adapters1_1 +---GATC-GGAA---GAGCTCGTATGCCGTCTTCTGCTTG +>FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAGCTC-----CCCT---------- +>Illumina_Genomic_DNA_Adapters1_2 +--ACACTCTT----------TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +CCA-AATCTTGAATTGTAGCTCCC---------CT---------- +>Illumina_Genomic_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACA-------CTCTTTCCCTACACGACGCTCTTCC +GATCT +>FC12044_91407_8_200_285_136 +-----------------CCAAATCTTGAATTGTAGCTC---CCCT--------------- +----- +>Illumina_Genomic_DNA_PCR_Primers1_2 +-CAAGCA----GAAGACGGCATACGAGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +CCAA--ATCTTGA-------ATTGTAGCTC--CC---CT +>Illumina_Genomic_DNA_sequencing_primer +--ACACTCTT----------TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +CCA-AATCTTGAATTGTAGCTCCC---------CT---------- +>Illumina_Paired_End_DNA_Adapters1_1 +GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-------------------- +>FC12044_91407_8_200_285_136 +---------------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_Paired_End_DNA_Adapters1_2 +--ACACTCTT----------TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +CCA-AATCTTGAATTGTAGCTCCC---------CT---------- +>Illumina_Paired_End_DNA_PCR_Primers1_1 +AATGATACGGCGACCACCGAGATCTACA-------CTCTTTCCCTACACGACGCTCTTCC +GATCT +>FC12044_91407_8_200_285_136 +-----------------CCAAATCTTGAATTGTAGCTC---CCCT--------------- +----- +>Illumina_Paired_End_DNA_PCR_Primers1_2 +CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC +T +>FC12044_91407_8_200_285_136 +------------------CCAAATC-----TTGAATTGTAGCT---CCCCT--------- +- +>Illumina_Paired_End_DNA_sequencing_primer_1 +--ACACTCTT----------TCCCTACACGACGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +CCA-AATCTTGAATTGTAGCTCCC---------CT---------- +>Illumina_Paired_End_DNA_sequencing_primer_2 +CGGTCTCGGCATTCC---TGCTGAA--------CCGCTCTTCCGATCT +>FC12044_91407_8_200_285_136 +-------------CCAAATCTTGAATTGTAGCTCCCCT---------- +>Illumina_DpnII_Gex_Adapters1_1 +---GATCGTCGGACTGTAGAACT---CTGAAC +>FC12044_91407_8_200_285_136 +CCAAATC-TTGAATTGTAG--CTCCCCT---- +>Illumina_DpnII_Gex_Adapters1_2 +--ACAGGTTCAGAGTTCTACAG-TCCGAC-- +>FC12044_91407_8_200_285_136 +CCAAATCTT--GAATTGT--AGCTCC--CCT +>Illumina_DpnII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGA---------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_DpnII_Gex_Adapters2_2 +------------TCGTA--TGCCGTCTTCTGCTTG +>FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAGCTCCCCT---------- +>Illumina_DpnII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA---------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_DpnII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA- +>FC12044_91407_8_200_285_136 +-----------------CCAAATCTT--GAATTGT--AGCTCCCCT +>Illumina_DpnII_Gex_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC---- +>FC12044_91407_8_200_285_136 +CCAAATCTT--GAATTGTA---------GCTCCCCT +>Illumina_NlaIII_Gex_Adapters1_1 +-------TCGGACTGTAGAACT---CTGAAC +>FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAG--CTCCCCT---- +>Illumina_NlaIII_Gex_Adapters1_2 +--ACAGGTTCAGAGTTCTACAG-TCCGACATG +>FC12044_91407_8_200_285_136 +CCAAATCTT--GAATTGT--AGCTCC--CCT- +>Illumina_NlaIII_Gex_Adapters2_1 +CAAGCAGAAGACGGCATACGANN-------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_NlaIII_Gex_Adapters2_2 +------------TCGTA--TGCCGTCTTCTGCTTG +>FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAGCTCCCCT---------- +>Illumina_NlaIII_Gex_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA---------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_NlaIII_Gex_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA- +>FC12044_91407_8_200_285_136 +-----------------CCAAATCTT--GAATTGT--AGCTCCCCT +>Illumina_NlaIII_Gex_sequencing_primer +CCGACAGGTTCAGAGTTCTACAG-TCCGACATG +>FC12044_91407_8_200_285_136 +-CCAAATCTT--GAATTGT--AGCTCC--CCT- +>Illumina_Small_RNA_RT_Primer +CAAGCAGAAGACGGCATACGA---------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_Small_RNA_5p_Adapter +GTTCAGAGTTCTACAGTCCGACGATC------------------ +>FC12044_91407_8_200_285_136 +-----------------CCAA--ATCTTGAATTGTAGCTCCCCT +>Illumina_Small_RNA_3p_Adapter +------------TCGTA--TGCCGTCTTCTGCTTGT +>FC12044_91407_8_200_285_136 +CCAAATCTTGAATTGTAGCTCCCCT----------- +>Illumina_Small_RNA_PCR_Primer_1 +CAAGCAGAAGACGGCATACGA---------------------- +>FC12044_91407_8_200_285_136 +------------------CCAAATCTTGAATTGTAGCTCCCCT +>Illumina_Small_RNA_PCR_Primer_2 +AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA- +>FC12044_91407_8_200_285_136 +-----------------CCAAATCTT--GAATTGT--AGCTCCCCT +>Illumina_Small_RNA_sequencing_primer +CGACAGGTTCAGAGTTCTACAGTCCGACGATC---- +>FC12044_91407_8_200_285_136 +CCAAATCTT--GAATTGTA---------GCTCCCCT
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/emboss_needleall_out.pair Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,18267 @@ +######################################## +# Program: needleall +# Rundate: Mon 16 Dec 2024 17:13:24 +# Commandline: needleall +# -asequence /tmp/saskia/tmpet25av5g/files/e/e/8/dataset_ee891bb1-6c31-453a-8e30-69544c761887.dat +# -bsequence /tmp/saskia/tmpet25av5g/files/0/7/0/dataset_07021fba-c6f2-478f-8665-0e6832dba409.dat +# -outfile /tmp/saskia/tmpet25av5g/job_working_directory/000/9/outputs/dataset_b9c62224-ff77-442f-a21f-1e30d4ffb6fc.dat +# -gapopen 10.0 +# -gapextend 0.5 +# -brief yes +# -aformat3 pair +# -auto +# -datafile EPAM30 +# -endopen 13.37 +# -endextend 2.5 +# -minscore 1.0 +# Align_format: pair +# Report_file: /tmp/saskia/tmpet25av5g/job_working_directory/000/9/outputs/dataset_b9c62224-ff77-442f-a21f-1e30d4ffb6fc.dat +######################################## + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 16/37 (43.2%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG---- 33 + |.| ||||| ||..|||..| .|| +FC12044_91407 1 GTT------AGCTC-----CCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACC--GAGATCTACACTCTTTCCCTACACGACGCT 48 + |.|| ||..||||| .||||.| ||. +FC12044_91407 1 ---GTTA--GCTCCCACCTTAAGATGT------TTA-------------- 25 + +Illumina_Geno 49 CTTCCGATCT 58 + +FC12044_91407 26 ---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 11/47 (23.4%) +# Similarity: 11/47 (23.4%) +# Gaps: 35/47 (74.5%) +# Score: 49.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACG--AGCTCTTCCGATCT----------- 34 + | ||||| || |.|| +FC12044_91407 1 -------------------GTTAGCTC--CC-ACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 33/45 (73.3%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCC------GAG------- 32 + ||| |||. || .|| +FC12044_91407 1 -------------GTT-AGCT------CCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACC--GAGATCTACACTCTTTCCCTACACGACGCT 48 + |.|| ||..||||| .||||.| ||. +FC12044_91407 1 ---GTTA--GCTCCCACCTTAAGATGT------TTA-------------- 25 + +Illumina_Pair 49 CTTCCGATCT 58 + +FC12044_91407 26 ---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 67 +# Identity: 15/67 (22.4%) +# Similarity: 15/67 (22.4%) +# Gaps: 48/67 (71.6%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGT---CTCGGCATTCCTGCTGAAC 47 + || ||| | +FC12044_91407 1 --------------------------GTTAGCTC---------------C 9 + +Illumina_Pair 48 CGCTCTTCCGATCT--- 61 + |.| |||..|||.| +FC12044_91407 10 CAC-CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 24/43 (55.8%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 CGGT---CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT--- 37 + || ||| ||.| |||..|||.| +FC12044_91407 1 --GTTAGCTC---------------CCAC-CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 9/42 (21.4%) +# Similarity: 9/42 (21.4%) +# Gaps: 33/42 (78.6%) +# Score: 34.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGAAC---------------- 26 + || ||| ||| | +FC12044_91407 1 ----GT-------TAG--CTC----CCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 10/40 (25.0%) +# Similarity: 10/40 (25.0%) +# Gaps: 29/40 (72.5%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAG-TCCGAC------------- 26 + ||| || |||.|| +FC12044_91407 1 -----------GTT----AGCTCCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_DpnI 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 11/28 (39.3%) +# Similarity: 11/28 (39.3%) +# Gaps: 10/28 (35.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATG---CCGTCTTCTGCTTG---- 21 + ||..| ||..|||..| .|| +FC12044_91407 1 --GTTAGCTCCCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_DpnI 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA 44 + |.|| ||..||||| ||.||| ||| || +FC12044_91407 1 ---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 33/45 (73.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAG-TCCGACGATC------------ 32 + ||| || |||.|| | +FC12044_91407 1 -------------GTT----AGCTCCCAC---CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 7/37 (18.9%) +# Similarity: 7/37 (18.9%) +# Gaps: 28/37 (75.7%) +# Score: 31.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCTGAAC---------------- 21 + ||. |.||| | +FC12044_91407 1 -------GTT-AGCTC----CCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 26/40 (65.0%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAG-TCCGAC------ATG---- 29 + ||| || |||.|| ||| +FC12044_91407 1 -----------GTT----AGCTCCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 9/36 (25.0%) +# Similarity: 9/36 (25.0%) +# Gaps: 24/36 (66.7%) +# Score: 26.5 +# +# +#======================================= + +Illumina_NlaI 1 ---------CAAGC----AGAAGACGGCATACGANN 23 + || | |||.|. .|| +FC12044_91407 1 GTTAGCTCCCA--CCTTAAGATGT----TTA----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 11/28 (39.3%) +# Similarity: 11/28 (39.3%) +# Gaps: 10/28 (35.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATG---CCGTCTTCTGCTTG---- 21 + ||..| ||..|||..| .|| +FC12044_91407 1 --GTTAGCTCCCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_NlaI 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA 44 + |.|| ||..||||| ||.||| ||| || +FC12044_91407 1 ---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 29/43 (67.4%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAG-TCCGAC------ATG---- 32 + ||| || |||.|| ||| +FC12044_91407 1 --------------GTT----AGCTCCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_Smal 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 11/39 (28.2%) +# Similarity: 11/39 (28.2%) +# Gaps: 27/39 (69.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAG-TCCGACGATC------------ 26 + ||| || |||.|| | +FC12044_91407 1 -------GTT----AGCTCCCAC---CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 9/28 (32.1%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATG---CCGTCTTCTGCTTGT--- 22 + ||..| ||..|||..| .||| +FC12044_91407 1 --GTTAGCTCCCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_Smal 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA 44 + |.|| ||..||||| ||.||| ||| || +FC12044_91407 1 ---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 33/45 (73.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAG-TCCGACGATC------------ 32 + ||| || |||.|| | +FC12044_91407 1 -------------GTT----AGCTCCCAC---CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATG-----CCGT-CTTCTGCTTG 33 + ||| |.|| ||.| |.||. ||| +FC12044_91407 1 -----------CTC-TGTGGCACCCCATCCCTCA-CTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 92.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCT---ACACTCTTTCCCTACACGACGC 47 + | ||| .|||.|..||||| | +FC12044_91407 1 ----------------C-----TCTGTGGCACCCCATCCCT--------C 21 + +Illumina_Geno 48 TCTTCCGATCT 58 + .||| +FC12044_91407 22 ACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 19/39 (48.7%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT----- 34 + |. |.|. ||| || |.|.||| || +FC12044_91407 1 CT--CTGT----GGC--AC---CCCATCC---CTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 21.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATG----CCGAG--------- 32 + | ||.| || ||.|. +FC12044_91407 1 -----------C---TCTG------TGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 92.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCT---ACACTCTTTCCCTACACGACGC 47 + | ||| .|||.|..||||| | +FC12044_91407 1 ----------------C-----TCTGTGGCACCCCATCCCT--------C 21 + +Illumina_Pair 48 TCTTCCGATCT 58 + .||| +FC12044_91407 22 ACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 16/64 (25.0%) +# Similarity: 16/64 (25.0%) +# Gaps: 42/64 (65.6%) +# Score: 86.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTC---GGCATTCCTGCTGAAC 47 + ||| ||||..||..| | +FC12044_91407 1 ----------------------------CTCTGTGGCACCCCATC----C 18 + +Illumina_Pair 48 CGCTCTTCCGATCT 61 + |.|.||| +FC12044_91407 19 CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 16/40 (40.0%) +# Similarity: 16/40 (40.0%) +# Gaps: 18/40 (45.0%) +# Score: 86.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTC---GGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + ||| ||||..||..| ||.|.||| +FC12044_91407 1 ----CTCTGTGGCACCCCATC----CCTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 21/36 (58.3%) +# Score: 51.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAA--------CTCTGAAC-- 26 + | .||||.|.| ||| || +FC12044_91407 1 ------C--TCTGTGGCACCCCATCCCTC---ACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 8/40 (20.0%) +# Similarity: 8/40 (20.0%) +# Gaps: 29/40 (72.5%) +# Score: 43.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGT------CCGAC-------- 26 + || |.|| ||..| +FC12044_91407 1 --------------CT-CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 14/28 (50.0%) +# Similarity: 14/28 (50.0%) +# Gaps: 10/28 (35.7%) +# Score: 60.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATG-----CCGT-CTTCTGCTTG 21 + || |.|| ||.| |.||. ||| +FC12044_91407 1 CTC-TGTGGCACCCCATCCCTCA-CTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 15/52 (28.8%) +# Similarity: 15/52 (28.8%) +# Gaps: 35/52 (67.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC-----GGCGACC---ACCGACAGGTTCAGAGTTCTACAGTCC 42 + | ||| ||| .|| | ||| ||. +FC12044_91407 1 -------CTCTGTGGC-ACCCCATCC--C----TCA-----CTT------ 25 + +Illumina_DpnI 43 GA 44 + +FC12044_91407 26 -- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 51.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGT----CCGACGATC-------- 32 + || |.|| || |.||| +FC12044_91407 1 ----------------CT-CTGTGGCACC--CCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAA--------CTCTGAAC-- 21 + | .||||.|.| ||| || +FC12044_91407 1 -C--TCTGTGGCACCCCATCCCTC---ACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 16/35 (45.7%) +# Score: 44.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACA----GTCC--GACATG 29 + | ||.|.| .|| .||| .||.| +FC12044_91407 1 -C----TCTGTG----GCACCCCATCCCTCACTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 10/29 (34.5%) +# Score: 24.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCAT-----ACGANN 23 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 14/28 (50.0%) +# Similarity: 14/28 (50.0%) +# Gaps: 10/28 (35.7%) +# Score: 60.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATG-----CCGT-CTTCTGCTTG 21 + || |.|| ||.| |.||. ||| +FC12044_91407 1 CTC-TGTGGCACCCCATCCCTCA-CTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 15/52 (28.8%) +# Similarity: 15/52 (28.8%) +# Gaps: 35/52 (67.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC-----GGCGACC---ACCGACAGGTTCAGAGTTCTACAGTCC 42 + | ||| ||| .|| | ||| ||. +FC12044_91407 1 -------CTCTGTGGC-ACCCCATCC--C----TCA-----CTT------ 25 + +Illumina_NlaI 43 GA 44 + +FC12044_91407 26 -- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 29/43 (67.4%) +# Score: 46.5 +# +# +#======================================= + +Illumina_NlaI 1 -----------CCGACAGGTTCAGAGTTCTACAGTCCGACATG 32 + || || || | | || ||.| +FC12044_91407 1 CTCTGTGGCACCC--CA---TC------C--C--TC--ACTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 11/38 (28.9%) +# Similarity: 11/38 (28.9%) +# Gaps: 25/38 (65.8%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGT----CCGACGATC-------- 26 + || |.|| || |.||| +FC12044_91407 1 ----------CT-CTGTGGCACC--CCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 11/29 (37.9%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATG-----CCGT-CTTCTGCTTGT 22 + || |.|| ||.| |.||. ||| +FC12044_91407 1 CTC-TGTGGCACCCCATCCCTCA-CTT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 15/52 (28.8%) +# Similarity: 15/52 (28.8%) +# Gaps: 35/52 (67.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC-----GGCGACC---ACCGACAGGTTCAGAGTTCTACAGTCC 42 + | ||| ||| .|| | ||| ||. +FC12044_91407 1 -------CTCTGTGGC-ACCCCATCC--C----TCA-----CTT------ 25 + +Illumina_Smal 43 GA 44 + +FC12044_91407 26 -- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGT----CCGACGATC-------- 32 + || |.|| || |.||| +FC12044_91407 1 ----------------CT-CTGTGGCACC--CCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 12/40 (30.0%) +# Similarity: 12/40 (30.0%) +# Gaps: 22/40 (55.0%) +# Score: 37.5 +# +# +#======================================= + +Illumina_Geno 1 GAT-------CGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + ||| |...|.| |||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAA---CGTA------------CATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 13/69 (18.8%) +# Similarity: 13/69 (18.8%) +# Gaps: 55/69 (79.7%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Geno 1 -----------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTA 39 + ||| |.||| |||| || +FC12044_91407 1 GATTTTTTAACAAT-AAACG---------------TACA---------TA 25 + +Illumina_Geno 40 CACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 31/45 (68.9%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Geno 1 ----------CAAGCAGAAGACG-GCATACGAGCTCTTCCGATCT 34 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 12/39 (30.8%) +# Similarity: 12/39 (30.8%) +# Gaps: 21/39 (53.8%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 GAT-------CGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + ||| | .|..|.| ||.|| .| +FC12044_91407 1 GATTTTTTAAC-AATAAAC-GTACA-----TA------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 13/69 (18.8%) +# Similarity: 13/69 (18.8%) +# Gaps: 55/69 (79.7%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Pair 1 -----------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTA 39 + ||| |.||| |||| || +FC12044_91407 1 GATTTTTTAACAAT-AAACG---------------TACA---------TA 25 + +Illumina_Pair 40 CACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 72 +# Identity: 11/72 (15.3%) +# Similarity: 11/72 (15.3%) +# Gaps: 58/72 (80.6%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Pair 1 ----------CAAGCAGAAGACG-GCATACGAGATCGGTCTCGGCATTCC 39 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--------------------- 25 + +Illumina_Pair 40 TGCTGAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ---------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 24/43 (55.8%) +# Score: 28.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAAC------CGCTCTTCCGATCT 37 + |.| .|| |.||| ||..|.| | +FC12044_91407 1 -GAT------TTT-----TTAACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 15/33 (45.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGAAC------- 26 + ||| |.|..|||..|.||| +FC12044_91407 1 GAT--------TTTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 14/33 (42.4%) +# Similarity: 14/33 (42.4%) +# Gaps: 15/33 (45.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCT-ACAGT--CCG-AC--- 26 + |.|| ||.| |||.| .|| || +FC12044_91407 1 ---GATT-----TTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 6/37 (16.2%) +# Similarity: 6/37 (16.2%) +# Gaps: 28/37 (75.7%) +# Score: 26.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------------TCGTATGCCGTCTTCTGCTTG 21 + .|||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAACGTA------------CATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 55 +# Identity: 12/55 (21.8%) +# Similarity: 12/55 (21.8%) +# Gaps: 41/55 (74.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 -----------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG 39 + ||| |.|| ||.|| || +FC12044_91407 1 GATTTTTTAACAAT-AAAC---------------GTACA------TA--- 25 + +Illumina_DpnI 40 TCCGA 44 + +FC12044_91407 26 ----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 15/36 (41.7%) +# Score: 48.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCT-ACAGTCCGACGATC--- 32 + |.|| ||.| |||.| ..|||..| +FC12044_91407 1 -----GATT-----TTTTAACAAT-AAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 11/28 (39.3%) +# Similarity: 11/28 (39.3%) +# Gaps: 10/28 (35.7%) +# Score: 47.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCTGAAC------- 21 + ||.|.|..|||..|.||| +FC12044_91407 1 ---GATTTTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 16/33 (48.5%) +# Similarity: 16/33 (48.5%) +# Gaps: 12/33 (36.4%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCT-ACAGT--CCG-ACATG 29 + |.|| ||.| |||.| .|| ||||. +FC12044_91407 1 ---GATT-----TTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 20/34 (58.8%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAGAAGACG-GCATACGANN 23 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 6/37 (16.2%) +# Similarity: 6/37 (16.2%) +# Gaps: 28/37 (75.7%) +# Score: 26.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------------TCGTATGCCGTCTTCTGCTTG 21 + .|||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAACGTA------------CATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 55 +# Identity: 12/55 (21.8%) +# Similarity: 12/55 (21.8%) +# Gaps: 41/55 (74.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 -----------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG 39 + ||| |.|| ||.|| || +FC12044_91407 1 GATTTTTTAACAAT-AAAC---------------GTACA------TA--- 25 + +Illumina_NlaI 40 TCCGA 44 + +FC12044_91407 26 ----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 15/36 (41.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCT-ACAGT--CCG-ACATG 32 + |.|| ||.| |||.| .|| ||||. +FC12044_91407 1 ------GATT-----TTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 13/31 (41.9%) +# Similarity: 13/31 (41.9%) +# Gaps: 11/31 (35.5%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCT--ACAGTCCGACGATC--- 26 + ||.||.| |||.| ..|||..| +FC12044_91407 1 -----GATTTTTTAACAAT-AAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 6/38 (15.8%) +# Similarity: 6/38 (15.8%) +# Gaps: 29/38 (76.3%) +# Score: 26.5 +# +# +#======================================= + +Illumina_Smal 1 ----------------TCGTATGCCGTCTTCTGCTTGT 22 + .|||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAACGTA------------CATA- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 55 +# Identity: 12/55 (21.8%) +# Similarity: 12/55 (21.8%) +# Gaps: 41/55 (74.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 -----------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG 39 + ||| |.|| ||.|| || +FC12044_91407 1 GATTTTTTAACAAT-AAAC---------------GTACA------TA--- 25 + +Illumina_Smal 40 TCCGA 44 + +FC12044_91407 26 ----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 15/36 (41.7%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCT-ACAGTCCGACGATC--- 32 + |.|| ||.| |||.| ..|||..| +FC12044_91407 1 -----GATT-----TTTTAACAAT-AAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 67.5 +# +# +#======================================= + +Illumina_Geno 1 GAT---CGGAAGAGCTCGTATGCCGTCTT---CTGCTTG 33 + |.| |.| ||| |||||| ||.| +FC12044_91407 1 GTTGCCCAG----GCT-------CGTCTTGAACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 16/61 (26.2%) +# Similarity: 16/61 (26.2%) +# Gaps: 39/61 (63.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCT-ACACGACGCTC 49 + . ||.|||. .|.|| || +FC12044_91407 1 -----------------------------G-TTGCCCAGGCTCG----TC 16 + +Illumina_Geno 50 TTCCGATCT-- 58 + || ||.|| +FC12044_91407 17 TT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 21/40 (52.5%) +# Score: 69.0 +# +# +#======================================= + +Illumina_Geno 1 ------CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + ||.|| .||.|.| ||.||| | +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 27/42 (64.3%) +# Score: 46.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTT---CAG------CAGGAA-TGCCGAG 32 + ||| ||| |..||| | || +FC12044_91407 1 -------------GTTGCCCAGGCTCGTCTTGAACT-CC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 16/61 (26.2%) +# Similarity: 16/61 (26.2%) +# Gaps: 39/61 (63.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCT-ACACGACGCTC 49 + . ||.|||. .|.|| || +FC12044_91407 1 -----------------------------G-TTGCCCAGGCTCG----TC 16 + +Illumina_Pair 50 TTCCGATCT-- 58 + || ||.|| +FC12044_91407 17 TT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 19/64 (29.7%) +# Similarity: 19/64 (29.7%) +# Gaps: 42/64 (65.6%) +# Score: 83.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGAT---CGGTCTCGGCATTCCTGCTGAAC 47 + |.| |.|.|||| | || ||||| +FC12044_91407 1 ---------------------GTTGCCCAGGCTCG---T--CT--TGAAC 22 + +Illumina_Pair 48 CGCTCTTCCGATCT 61 + ||| +FC12044_91407 23 ------TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 17/43 (39.5%) +# Similarity: 17/43 (39.5%) +# Gaps: 24/43 (55.8%) +# Score: 82.5 +# +# +#======================================= + +Illumina_Pair 1 ------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + |.|.|||| | || ||||| ||| +FC12044_91407 1 GTTGCCCAGGCTCG---T--CT--TGAAC------TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 14/35 (40.0%) +# Similarity: 14/35 (40.0%) +# Gaps: 19/35 (54.3%) +# Score: 77.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------GATCGTCGGACTGTAGAACTCTGAAC 26 + |.||||| |.|||||| | +FC12044_91407 1 GTTGCCCAGGCTCGTC------TTGAACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 63.0 +# +# +#======================================= + +Illumina_DpnI 1 -----ACAGGTTCAGAGTTCT---ACAGTCCGAC 26 + .||||.||. ||| || ||| +FC12044_91407 1 GTTGCCCAGGCTCG----TCTTGAAC--TCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 14/31 (45.2%) +# Similarity: 14/31 (45.2%) +# Gaps: 16/31 (51.6%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGC-------CGTCTT---CTGCTTG 21 + || ||| |||||| ||.| +FC12044_91407 1 --GT-TGCCCAGGCTCGTCTTGAACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 75.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCT---ACAGTCCGA 44 + |.|.| ||| |.| ||. ||| || ||| +FC12044_91407 1 ---GTTGC-----CCA--GGC----TCG----TCTTGAAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 68.5 +# +# +#======================================= + +Illumina_DpnI 1 -----CGACAGGTTCAGAGTTCT---ACAGTCCGACGATC 32 + | ||||.||. ||| || ||| +FC12044_91407 1 GTTGCC--CAGGCTCG----TCTTGAAC--TCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 61.0 +# +# +#======================================= + +Illumina_NlaI 1 -----------TCGGACTGTAGAACTCTGAAC 21 + || |.| |.|||||| | +FC12044_91407 1 GTTGCCCAGGCTC-GTC--TTGAACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 20/37 (54.1%) +# Score: 63.0 +# +# +#======================================= + +Illumina_NlaI 1 -----ACAGGTTCAGAGTTCT---ACAGTCCGACATG 29 + .||||.||. ||| || ||| +FC12044_91407 1 GTTGCCCAGGCTCG----TCTTGAAC--TCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 20/34 (58.8%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAGAAGACGGCATA-----CGANN 23 + ||.|| .||.|.|. | +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTTGAACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 14/31 (45.2%) +# Similarity: 14/31 (45.2%) +# Gaps: 16/31 (51.6%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGC-------CGTCTT---CTGCTTG 21 + || ||| |||||| ||.| +FC12044_91407 1 --GT-TGCCCAGGCTCGTCTTGAACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 75.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCT---ACAGTCCGA 44 + |.|.| ||| |.| ||. ||| || ||| +FC12044_91407 1 ---GTTGC-----CCA--GGC----TCG----TCTTGAAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 78.5 +# +# +#======================================= + +Illumina_NlaI 1 ----CCGACAGGTTCAGAGTTCT---ACAGTCCGACATG 32 + || ||||.||. ||| || ||| +FC12044_91407 1 GTTGCC--CAGGCTCG----TCTTGAAC--TCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 15/34 (44.1%) +# Similarity: 15/34 (44.1%) +# Gaps: 17/34 (50.0%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Smal 1 GTT---CAGAGT--TCT---ACAGTCCGACGATC 26 + ||| |||..| ||| || ||| +FC12044_91407 1 GTTGCCCAGGCTCGTCTTGAAC--TCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 17/32 (53.1%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGC-------CGTCTT---CTGCTTGT 22 + || ||| |||||| ||.| +FC12044_91407 1 --GT-TGCCCAGGCTCGTCTTGAACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 75.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCT---ACAGTCCGA 44 + |.|.| ||| |.| ||. ||| || ||| +FC12044_91407 1 ---GTTGC-----CCA--GGC----TCG----TCTTGAAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Smal 1 -----CGACAGGTTCAGAGTTCT---ACAGTCCGACGATC 32 + | ||||.||. ||| || ||| +FC12044_91407 1 GTTGCC--CAGGCTCG----TCTTGAAC--TCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 18/37 (48.6%) +# Similarity: 18/37 (48.6%) +# Gaps: 16/37 (43.2%) +# Score: 55.0 +# +# +#======================================= + +Illumina_Geno 1 -GATCGGAAGAGCTCGTATGCCGT--C-TTCTGCTTG 33 + ||| .|||| |||.| || | |.||| +FC12044_91407 1 TGAT-TGAAG-----GTAGG--GTAGCATACTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Geno 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATC---TA-CACTCTTTCCCTACACGACG 46 + ||||. || ||.|. || || |||. | +FC12044_91407 1 --TGATT----GA------AGGTAGGGTAGCA---------TACT-G--- 25 + +Illumina_Geno 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 27/43 (62.8%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Geno 1 -----CAAGCAGAAGACG---GCATAC-GAGCTCTTCCGATCT 34 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG-------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Geno 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 19/35 (54.3%) +# Similarity: 19/35 (54.3%) +# Gaps: 13/35 (37.1%) +# Score: 56.5 +# +# +#======================================= + +Illumina_Pair 1 -GATCGGAAG--AGCGGTTCAGCAGGAATGCCGAG 32 + ||| .|||| || ||| ||| ||.|.| +FC12044_91407 1 TGAT-TGAAGGTAG-GGT--AGC----ATACTG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Pair 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATC---TA-CACTCTTTCCCTACACGACG 46 + ||||. || ||.|. || || |||. | +FC12044_91407 1 --TGATT----GA------AGGTAGGGTAGCA---------TACT-G--- 25 + +Illumina_Pair 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 19/62 (30.6%) +# Similarity: 19/62 (30.6%) +# Gaps: 38/62 (61.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGA-AGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG 49 + .|| .||.|| || | ||| .||||. ||| +FC12044_91407 1 -----TGATTGAAGG--TA-G-----GGT---AGCATA-CTG-------- 25 + +Illumina_Pair 50 CTCTTCCGATCT 61 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Pair 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 10/49 (20.4%) +# Similarity: 10/49 (20.4%) +# Gaps: 36/49 (73.5%) +# Score: 38.0 +# +# +#======================================= + +Illumina_Pair 1 ------------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + .||| .||||. ||| +FC12044_91407 1 TGATTGAAGGTAGGGT---AGCATA-CTG-------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 14/31 (45.2%) +# Similarity: 14/31 (45.2%) +# Gaps: 11/31 (35.5%) +# Score: 49.0 +# +# +#======================================= + +Illumina_DpnI 1 -----GATCGTCGGACTGTAGAACTCTGAAC 26 + ||..||.|| ||||.|..||| +FC12044_91407 1 TGATTGAAGGTAGG---GTAGCATACTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 50.5 +# +# +#======================================= + +Illumina_DpnI 1 ------ACAGGTTCAGAGTT-C-TACAGTCCGAC 26 + | |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 30.0 +# +# +#======================================= + +Illumina_DpnI 1 -------TCGTATGCCGT--C-TTCTGCTTG 21 + ..|||.| || | |.||| +FC12044_91407 1 TGATTGAAGGTAGG--GTAGCATACTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 19/46 (41.3%) +# Similarity: 19/46 (41.3%) +# Gaps: 23/46 (50.0%) +# Score: 66.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTT-C-TACAGTCCGA 44 + ||||. || |||| ||.||. | |||.| +FC12044_91407 1 --TGATT----GA--------AGGT--AGGGTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 48.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAGGTTCAGAGTT-C-TACAGTCCGACGATC 32 + .|| |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 ----------TCGGACTGTAGAACTCTGAAC 21 + |.|| ||||.|..||| +FC12044_91407 1 TGATTGAAGGTAGG---GTAGCATACTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 20/37 (54.1%) +# Score: 50.5 +# +# +#======================================= + +Illumina_NlaI 1 ------ACAGGTTCAGAGTT-C-TACAGTCCGACATG 29 + | |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 16/32 (50.0%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 -----CAAGCAGAAGACG---GCATAC-GANN 23 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 -------TCGTATGCCGT--C-TTCTGCTTG 21 + ..|||.| || | |.||| +FC12044_91407 1 TGATTGAAGGTAGG--GTAGCATACTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 19/46 (41.3%) +# Similarity: 19/46 (41.3%) +# Gaps: 23/46 (50.0%) +# Score: 66.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTT-C-TACAGTCCGA 44 + ||||. || |||| ||.||. | |||.| +FC12044_91407 1 --TGATT----GA--------AGGT--AGGGTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 21/39 (53.8%) +# Score: 46.0 +# +# +#======================================= + +Illumina_NlaI 1 -----CCGACAGGTTCAGAGTT-C-TACAGTCCGACATG 32 + || |||| ||.||. | |||.| +FC12044_91407 1 TGATT--GA-AGGT--AGGGTAGCATACTG--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 11/37 (29.7%) +# Similarity: 11/37 (29.7%) +# Gaps: 23/37 (62.2%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 ---------GTTCAGAGTT-C-TACAGTCCGACGATC 26 + || ||.||. | |||.| +FC12044_91407 1 TGATTGAAGGT--AGGGTAGCATACTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 17/32 (53.1%) +# Score: 30.0 +# +# +#======================================= + +Illumina_Smal 1 -------TCGTATGCCGT--C-TTCTGCTTGT 22 + ..|||.| || | |.||| +FC12044_91407 1 TGATTGAAGGTAGG--GTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 19/46 (41.3%) +# Similarity: 19/46 (41.3%) +# Gaps: 23/46 (50.0%) +# Score: 66.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTT-C-TACAGTCCGA 44 + ||||. || |||| ||.||. | |||.| +FC12044_91407 1 --TGATT----GA--------AGGT--AGGGTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAGGTTCAGAGTT-C-TACAGTCCGACGATC 32 + .|| |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTAT---GCCG-----TCTTCTGCTTG 33 + ||||..|| ||.| .| ||| +FC12044_91407 1 ----------GCTCCAATAGCGCAGAGGAAAC--CTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCA------CCGAGATCTACACTCTTTCCCTACACGA 44 + || .||| |.|||. |.| |||. +FC12044_91407 1 ---------GC-TCCAATAGCGCAGAGG---AAA-------CCTG----- 25 + +Illumina_Geno 45 CGCTCTTCCGATCT 58 + +FC12044_91407 26 -------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 27/43 (62.8%) +# Score: 58.5 +# +# +#======================================= + +Illumina_Geno 1 ----CAA----GCAGAAGACGGCATACGAGCTCTTCCGATCT- 34 + ||| |||||.| |.|| || +FC12044_91407 1 GCTCCAATAGCGCAGAGG-----AAAC-------------CTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 20/33 (60.6%) +# Similarity: 20/33 (60.6%) +# Gaps: 9/33 (27.3%) +# Score: 68.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCC-GAG 32 + |.|| .||.|||| ||| |||||. || | +FC12044_91407 1 GCTC-CAATAGCG---CAG-AGGAAA-CCTG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCA------CCGAGATCTACACTCTTTCCCTACACGA 44 + || .||| |.|||. |.| |||. +FC12044_91407 1 ---------GC-TCCAATAGCGCAGAGG---AAA-------CCTG----- 25 + +Illumina_Pair 45 CGCTCTTCCGATCT 58 + +FC12044_91407 26 -------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 14/69 (20.3%) +# Similarity: 14/69 (20.3%) +# Gaps: 52/69 (75.4%) +# Score: 64.0 +# +# +#======================================= + +Illumina_Pair 1 ----CAA----GCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGC 42 + ||| |||||.| |..|||| +FC12044_91407 1 GCTCCAATAGCGCAGAGG------------------------AAACCTG- 25 + +Illumina_Pair 43 TGAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 16/44 (36.4%) +# Similarity: 16/44 (36.4%) +# Gaps: 26/44 (59.1%) +# Score: 61.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATT--CCTGCTG----AACC-GCTCTTCCGATCT 37 + | ||| ||.| | ||.| |||| | +FC12044_91407 1 -G--CTC--CAATAGC--GCAGAGGAAACCTG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 19/35 (54.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAG---------AACTCTGAAC 26 + |.|| |..||| ||| ||| +FC12044_91407 1 GCTC------CAATAGCGCAGAGGAAAC-CTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 12/35 (34.3%) +# Similarity: 12/35 (34.3%) +# Gaps: 19/35 (54.3%) +# Score: 31.5 +# +# +#======================================= + +Illumina_DpnI 1 ------ACAG-GTTCAGAG--TTCTACAGTCCGAC 26 + |.|| | ||||| ..| |.| +FC12044_91407 1 GCTCCAATAGCG--CAGAGGAAAC--CTG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 34.5 +# +# +#======================================= + +Illumina_DpnI 1 --TCGTAT---GCCG-----TCTTCTGCTTG 21 + ||..|| ||.| .| ||| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAAC--CTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 25/47 (53.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAG-GTTCAGAG--TTCTACAGTCCGA 44 + . |.||.|.|| | ||||| ..| |.| +FC12044_91407 1 ------------G-CTCCAATAGCG--CAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 13/39 (33.3%) +# Similarity: 13/39 (33.3%) +# Gaps: 21/39 (53.8%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAG-GTTCAGAG--TTCTACAGTCCGACGATC 32 + |.|.|| | ||||| ..| |.| +FC12044_91407 1 GCTCCAATAGCG--CAGAGGAAAC--CTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 --TCGGACTGTAG---------AACTCTGAAC 21 + || |..||| ||| ||| +FC12044_91407 1 GCTC---CAATAGCGCAGAGGAAAC-CTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 12/38 (31.6%) +# Similarity: 12/38 (31.6%) +# Gaps: 22/38 (57.9%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTC-CG--------ACATG 29 + | ||.||.|. || ||.|| +FC12044_91407 1 -----------G--CTCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 12/34 (35.3%) +# Similarity: 12/34 (35.3%) +# Gaps: 20/34 (58.8%) +# Score: 53.5 +# +# +#======================================= + +Illumina_NlaI 1 ----CAA----GCAGAAGA---CGGCATACGANN 23 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 34.5 +# +# +#======================================= + +Illumina_NlaI 1 --TCGTAT---GCCG-----TCTTCTGCTTG 21 + ||..|| ||.| .| ||| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAAC--CTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_NlaI 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 25/47 (53.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAG-GTTCAGAG--TTCTACAGTCCGA 44 + . |.||.|.|| | ||||| ..| |.| +FC12044_91407 1 ------------G-CTCCAATAGCG--CAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 15/36 (41.7%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAG-GTTCAGAGTTCTACAGTCCGACATG 32 + ||.|.|| | |||||. |..|| || +FC12044_91407 1 GCTCCAATAGCG--CAGAGG-----AAACC----TG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_Smal 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 9/39 (23.1%) +# Similarity: 9/39 (23.1%) +# Gaps: 27/39 (69.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGTCCGA---CG-------ATC--- 26 + .| |||.| || |.| +FC12044_91407 1 ------------GC--TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 17/32 (53.1%) +# Score: 34.5 +# +# +#======================================= + +Illumina_Smal 1 --TCGTAT---GCCG-----TCTTCTGCTTGT 22 + ||..|| ||.| .| ||| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_Smal 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 25/47 (53.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAG-GTTCAGAG--TTCTACAGTCCGA 44 + . |.||.|.|| | ||||| ..| |.| +FC12044_91407 1 ------------G-CTCCAATAGCG--CAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 13/39 (33.3%) +# Similarity: 13/39 (33.3%) +# Gaps: 21/39 (53.8%) +# Score: 39.5 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAG-GTTCAGAG--TTCTACAGTCCGACGATC 32 + |.|.|| | ||||| ..| |.| +FC12044_91407 1 GCTCCAATAGCG--CAGAGGAAAC--CTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 8/50 (16.0%) +# Similarity: 8/50 (16.0%) +# Gaps: 42/50 (84.0%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG----------------- 33 + | ||||||| +FC12044_91407 1 ---------------------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 10/70 (14.3%) +# Similarity: 10/70 (14.3%) +# Gaps: 57/70 (81.4%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT-C 49 + ||| | +FC12044_91407 1 ---------------------------------------------GCTGC 5 + +Illumina_Geno 50 TTCCGA-TCT---------- 58 + ||..|| .|| +FC12044_91407 6 TTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 27/43 (62.8%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Geno 1 ----C-----AAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + | |.||.|| ||||. ||| +FC12044_91407 1 GCTGCTTGGGAGGCTGA----GGCAG--GAG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 13/35 (37.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Pair 1 ---GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + |.|.||.|| ||.|. .||||||. +FC12044_91407 1 GCTGCTTGGGAG-GCTGA--GGCAGGAG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 10/70 (14.3%) +# Similarity: 10/70 (14.3%) +# Gaps: 57/70 (81.4%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT-C 49 + ||| | +FC12044_91407 1 ---------------------------------------------GCTGC 5 + +Illumina_Pair 50 TTCCGA-TCT---------- 58 + ||..|| .|| +FC12044_91407 6 TTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 13/70 (18.6%) +# Similarity: 13/70 (18.6%) +# Gaps: 54/70 (77.1%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Pair 1 ----C-----AAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTG 41 + | |.||.|| ||||. ||| +FC12044_91407 1 GCTGCTTGGGAGGCTGA----GGCAG--GAG------------------- 25 + +Illumina_Pair 42 CTGAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 -------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 13/45 (28.9%) +# Similarity: 13/45 (28.9%) +# Gaps: 28/45 (62.2%) +# Score: 38.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCT---GAACCGCTCTTCCGA-TCT---- 37 + . ||||| ||. ||| || .|. +FC12044_91407 1 ----------G---CTGCTTGGGAG--GCT-----GAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 10/39 (25.6%) +# Similarity: 10/39 (25.6%) +# Gaps: 27/39 (69.2%) +# Score: 40.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACT-------CTGA-AC----- 26 + .||| || |||| .| +FC12044_91407 1 ---------GCTG-----CTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 13/32 (40.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTT------CAGAGTTCTACAGTCCGAC 26 + .|.|.|| |.||| .||| ||. +FC12044_91407 1 GCTGCTTGGGAGGCTGAG----GCAG---GAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTCTTCTGCTTG----------------- 21 + | ||||||| +FC12044_91407 1 ---------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + |.|.| ||| || |.|||| || +FC12044_91407 1 ---GCTGCTTGGGAGGCTGA-----GGCAGG---AG-------------- 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 40.0 +# +# +#======================================= + +Illumina_DpnI 1 -CGAC-----AGGTTCAGAGTTCTACAGTCCGACGATC 32 + |..| ||| |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGG--CTGAG----GCAG---GA-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 40.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACT-------CTGA-AC----- 21 + .||| || |||| .| +FC12044_91407 1 ----GCTG-----CTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 14/35 (40.0%) +# Similarity: 14/35 (40.0%) +# Gaps: 16/35 (45.7%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTT------CAGAGTTCTACAGTCCGACATG 29 + .|.|.|| |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGGCTGAG----GCAG---GA---G 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 28/38 (73.7%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------C--AAGCAGAAGACGGCATACGANN 23 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTCTTCTGCTTG----------------- 21 + | ||||||| +FC12044_91407 1 ---------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + |.|.| ||| || |.|||| || +FC12044_91407 1 ---GCTGCTTGGGAGGCTGA-----GGCAGG---AG-------------- 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 14/38 (36.8%) +# Similarity: 14/38 (36.8%) +# Gaps: 19/38 (50.0%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTT------CAGAGTTCTACAGTCCGACATG 32 + .|.|.|| |.||| .||| || | +FC12044_91407 1 ---GCTGCTTGGGAGGCTGAG----GCAG---GA---G 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 30.0 +# +# +#======================================= + +Illumina_Smal 1 ---G-TT------CAGAGTTCTACAGTCCGACGATC 26 + | || |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGGCTGAG----GCAG---GA-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 9/38 (23.7%) +# Similarity: 9/38 (23.7%) +# Gaps: 29/38 (76.3%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCTTCTGCTTG------T---------- 22 + | ||||||| | +FC12044_91407 1 ---------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + |.|.| ||| || |.|||| || +FC12044_91407 1 ---GCTGCTTGGGAGGCTGA-----GGCAGG---AG-------------- 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 40.0 +# +# +#======================================= + +Illumina_Smal 1 -CGAC-----AGGTTCAGAGTTCTACAGTCCGACGATC 32 + |..| ||| |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGG--CTGAG----GCAG---GA-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 26/42 (61.9%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGT---ATGC------CGTCTTCTGCTTG 33 + |||.|| | ||.| ||.|| +FC12044_91407 1 -------AGACCT--TTGGATACAATGAACGACT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Geno 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 17/66 (25.8%) +# Similarity: 17/66 (25.8%) +# Gaps: 49/66 (74.2%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TAC-----AC 42 + ||| | |||| ||| || +FC12044_91407 1 -------------------AGA-C------CTTT---GGATACAATGAAC 21 + +Illumina_Geno 43 GACGCTCTTCCGATCT 58 + || || +FC12044_91407 22 GA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 17/41 (41.5%) +# Similarity: 17/41 (41.5%) +# Gaps: 23/41 (56.1%) +# Score: 63.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGAC----GGCATAC---GAGCTCTTCCGATCT 34 + |||| || |||| ||. ||| || +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAA------CGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Geno 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 17/37 (45.9%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGC----GGTTCAGCAGGAATGCCGAG- 32 + |||.| |.|.|| |.||| |||. +FC12044_91407 1 -------AGACCTTTGGATACA--ATGAA---CGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Pair 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 17/66 (25.8%) +# Similarity: 17/66 (25.8%) +# Gaps: 49/66 (74.2%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TAC-----AC 42 + ||| | |||| ||| || +FC12044_91407 1 -------------------AGA-C------CTTT---GGATACAATGAAC 21 + +Illumina_Pair 43 GACGCTCTTCCGATCT 58 + || || +FC12044_91407 22 GA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 17/66 (25.8%) +# Similarity: 17/66 (25.8%) +# Gaps: 46/66 (69.7%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGAC----GGCATACGA-GATCGGTCTCGGCATTCCTGCTGA 45 + |||| || ||||.| ||.| |.|| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAAC-GACT--------------- 25 + +Illumina_Pair 46 ACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Pair 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 12/45 (26.7%) +# Similarity: 12/45 (26.7%) +# Gaps: 28/45 (62.2%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCC------TGC--TGAACCGCTCTTCCGATCT 37 + |..|| |.| |||||..|| +FC12044_91407 1 ----------AGACCTTTGGATACAATGAACGACT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 13/32 (40.6%) +# Score: 62.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGA-CTGTAGA-ACTCTGAAC---- 26 + .|| ||.|.|| ||..||||| +FC12044_91407 1 -------AGACCTTTGGATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 53.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTC-------TACA--GTCCGAC- 26 + ||| | |||| |..|||| +FC12044_91407 1 --------AGA---CCTTTGGATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 ------TCGTATGC------CGTCTTCTGCTTG 21 + |.|.||.| ||.|| +FC12044_91407 1 AGACCTTTGGATACAATGAACGACT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 56.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA--GTCCGA-- 44 + . |||| ||..|| |||| |..||| +FC12044_91407 1 ---------A-GACC---------TTTGGA----TACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTC-------TACAGTCCG-ACGATC- 32 + ||| | ||||.| | |||| | +FC12044_91407 1 ----------AGA---CCTTTGGATACAAT--GAACGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 62.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGA-CTGTAGA-ACTCTGAAC---- 21 + .|| ||.|.|| ||..||||| +FC12044_91407 1 --AGACCTTTGGATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 13/38 (34.2%) +# Similarity: 13/38 (34.2%) +# Gaps: 22/38 (57.9%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTC-------TACA--GTCCGACATG 29 + ||| | |||| |..||||. +FC12044_91407 1 --------AGA---CCTTTGGATACAATGAACGACT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 20/34 (58.8%) +# Score: 46.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC----GGCATACGANN------- 23 + |||| || ||||.|.. +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_NlaI 1 ------TCGTATGC------CGTCTTCTGCTTG 21 + |.|.||.| ||.|| +FC12044_91407 1 AGACCTTTGGATACAATGAACGACT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA--GTCCGA-- 44 + . |||| ||..|| |||| |..||| +FC12044_91407 1 ---------A-GACC---------TTTGGA----TACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 17/37 (45.9%) +# Score: 60.5 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAGGTTCAGAGTTCTACA--GTCCGACATG 32 + || ||..|| |||| |..||||. +FC12044_91407 1 AGACC------TTTGGA----TACAATGAACGACT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 19/35 (54.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTC-------TACAGTCCG-ACGATC- 26 + ||| | ||||.| | |||| | +FC12044_91407 1 ----AGA---CCTTTGGATACAAT--GAACGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 21/34 (61.8%) +# Score: 39.5 +# +# +#======================================= + +Illumina_Smal 1 ------TCGTATGC------CGTCTTCTGCTTGT 22 + |.|.||.| ||.|| +FC12044_91407 1 AGACCTTTGGATACAATGAACGACT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 56.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA--GTCCGA-- 44 + . |||| ||..|| |||| |..||| +FC12044_91407 1 ---------A-GACC---------TTTGGA----TACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTC-------TACAGTCCG-ACGATC- 32 + ||| | ||||.| | |||| | +FC12044_91407 1 ----------AGA---CCTTTGGATACAAT--GAACGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 30/44 (68.2%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Geno 1 -----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + || |.||.|| ||||| +FC12044_91407 1 AGGGAATTTTAGA---GGAGGGC-----TGCCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 15/62 (24.2%) +# Similarity: 15/62 (24.2%) +# Gaps: 41/62 (66.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 ----AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + |||..|| |.| |||..||.| || +FC12044_91407 1 AGGGAATTTTA--GAG------GAGGGCTGC--------------CG--- 25 + +Illumina_Geno 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 19/39 (48.7%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCAT-----ACGAGCTCTTCCGATCT 34 + || ||.|| |.|||..||.||| +FC12044_91407 1 --------AG--GGAATTTTAGAGGAGGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 19/38 (50.0%) +# Score: 61.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGG---TTCAGCAGGAA---TGCCGAG 32 + || || ||.|| ||||. ||||| +FC12044_91407 1 ---------AG-GGAATTTTAG-AGGAGGGCTGCCG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 15/62 (24.2%) +# Similarity: 15/62 (24.2%) +# Gaps: 41/62 (66.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Pair 1 ----AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + |||..|| |.| |||..||.| || +FC12044_91407 1 AGGGAATTTTA--GAG------GAGGGCTGC--------------CG--- 25 + +Illumina_Pair 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 20/61 (32.8%) +# Similarity: 20/61 (32.8%) +# Gaps: 36/61 (59.0%) +# Score: 56.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + || ||.||...||| ||. || |||| ||| +FC12044_91407 1 --------AG--GGAATTTTAGA--GGA---GG-------GCTG--CCG- 25 + +Illumina_Pair 51 TCTTCCGATCT 61 + +FC12044_91407 26 ----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 14/46 (30.4%) +# Similarity: 14/46 (30.4%) +# Gaps: 30/46 (65.2%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCT---------GCTGAACCGCTCTTCCGATCT 37 + .| ||.||| | |||| ||| +FC12044_91407 1 -AG----GGAATT--TTAGAGGAGGGCTG--CCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 9/41 (22.0%) +# Similarity: 9/41 (22.0%) +# Gaps: 31/41 (75.6%) +# Score: 28.5 +# +# +#======================================= + +Illumina_DpnI 1 --------------GATCGTCGGACTGTAGAACTCTGAAC- 26 + || ||.||| | | +FC12044_91407 1 AGGGAATTTTAGAGGA-----GGGCTG-----C------CG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTA--------CAGTCCGAC 26 + ||| ||.||.|| |.| ||| +FC12044_91407 1 --AGG----GAATTTTAGAGGAGGGCTG-CCG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 34.5 +# +# +#======================================= + +Illumina_DpnI 1 --------TCGTA---------TGCCGTCTTCTGCTTG 21 + | || ||||| +FC12044_91407 1 AGGGAATTT--TAGAGGAGGGCTGCCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 14/52 (26.9%) +# Similarity: 14/52 (26.9%) +# Gaps: 35/52 (67.3%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTA--------CAGTCC 42 + ||| ||.||.|| |.| || +FC12044_91407 1 ---------------------AGG----GAATTTTAGAGGAGGGCTG-CC 24 + +Illumina_DpnI 43 GA 44 + | +FC12044_91407 25 G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTA--------CAGTCCGACGATC 32 + ||| ||.||.|| |.| ||| +FC12044_91407 1 ----AGG----GAATTTTAGAGGAGGGCTG-CCG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 22.5 +# +# +#======================================= + +Illumina_NlaI 1 ------TCGGACTGTAGA-----ACT-CTGAAC- 21 + | |.|||| .|| | | +FC12044_91407 1 AGGGAAT-----TTTAGAGGAGGGCTGC----CG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 20/37 (54.1%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTA--------CAGTCCGACATG 29 + ||| ||.||.|| |.| ||| +FC12044_91407 1 --AGG----GAATTTTAGAGGAGGGCTG-CCG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 12/30 (40.0%) +# Score: 35.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAG-------CAGAAGACGGCATACGANN 23 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 34.5 +# +# +#======================================= + +Illumina_NlaI 1 --------TCGTA---------TGCCGTCTTCTGCTTG 21 + | || ||||| +FC12044_91407 1 AGGGAATTT--TAGAGGAGGGCTGCCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 14/52 (26.9%) +# Similarity: 14/52 (26.9%) +# Gaps: 35/52 (67.3%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTA--------CAGTCC 42 + ||| ||.||.|| |.| || +FC12044_91407 1 ---------------------AGG----GAATTTTAGAGGAGGGCTG-CC 24 + +Illumina_NlaI 43 GA 44 + | +FC12044_91407 25 G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTA--------CAGTCCGACATG 32 + ||| ||.||.|| |.| ||| +FC12044_91407 1 -----AGG----GAATTTTAGAGGAGGGCTG-CCG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Smal 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 21/36 (58.3%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCA--GAGTTCTA--------CAGTCCGACGATC 26 + | ||.||.|| |.| ||| +FC12044_91407 1 ----AGGGAATTTTAGAGGAGGGCTG-CCG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 8/39 (20.5%) +# Similarity: 8/39 (20.5%) +# Gaps: 31/39 (79.5%) +# Score: 34.5 +# +# +#======================================= + +Illumina_Smal 1 --------TCGTA---------TGCCGTCTTCTGCTTGT 22 + | || ||||| +FC12044_91407 1 AGGGAATTT--TAGAGGAGGGCTGCCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Smal 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 14/52 (26.9%) +# Similarity: 14/52 (26.9%) +# Gaps: 35/52 (67.3%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTA--------CAGTCC 42 + ||| ||.||.|| |.| || +FC12044_91407 1 ---------------------AGG----GAATTTTAGAGGAGGGCTG-CC 24 + +Illumina_Smal 43 GA 44 + | +FC12044_91407 25 G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTA--------CAGTCCGACGATC 32 + ||| ||.||.|| |.| ||| +FC12044_91407 1 ----AGG----GAATTTTAGAGGAGGGCTG-CCG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 28/43 (65.1%) +# Score: 50.0 +# +# +#======================================= + +Illumina_Geno 1 ----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + |.||.| ||| | ||| || +FC12044_91407 1 TCTCCATGTTGGTCAG----GCT-G------GTC-TC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 65.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTT--TCCCTACACGACGCT 48 + |||.|| |.|| || |.||| +FC12044_91407 1 ----------------------TCTCCA-TGTTGGTC--------AGGCT 19 + +Illumina_Geno 49 CTTCCGATCT- 58 + |.||| +FC12044_91407 20 -----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 13/44 (29.5%) +# Similarity: 13/44 (29.5%) +# Gaps: 29/44 (65.9%) +# Score: 53.5 +# +# +#======================================= + +Illumina_Geno 1 ----CAAG-----CAGAAGACGGCATACGAGCTCTTCCGATCT- 34 + ||.| ||| ||| |.||| +FC12044_91407 1 TCTCCATGTTGGTCAG--------------GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Pair 1 ----------GATCGGAAGAGC-GGT-TCAGCAGGAATGCCGAG 32 + |.||.| || ||| || +FC12044_91407 1 TCTCCATGTTGGTCAG----GCTGGTCTC--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 65.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTT--TCCCTACACGACGCT 48 + |||.|| |.|| || |.||| +FC12044_91407 1 ----------------------TCTCCA-TGTTGGTC--------AGGCT 19 + +Illumina_Pair 49 CTTCCGATCT- 58 + |.||| +FC12044_91407 20 -----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 16/66 (24.2%) +# Similarity: 16/66 (24.2%) +# Gaps: 46/66 (69.7%) +# Score: 81.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCAT-----TCCTGCTGA 45 + |||| ||| ||..||||. +FC12044_91407 1 ---------------------------TCTC--CATGTTGGTCAGGCTGG 21 + +Illumina_Pair 46 ACCGCTCTTCCGATCT 61 + . ||| +FC12044_91407 22 T---CTC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 16/42 (38.1%) +# Similarity: 16/42 (38.1%) +# Gaps: 22/42 (52.4%) +# Score: 81.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCAT-----TCCTGCTGAACCGCTCTTCCGATCT 37 + |||| ||| ||..||||.. ||| +FC12044_91407 1 ---TCTC--CATGTTGGTCAGGCTGGT---CTC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 15/33 (45.5%) +# Score: 64.0 +# +# +#======================================= + +Illumina_DpnI 1 -------GATCGTCGGACTGTAGAACTCTGAAC 26 + |.|.|||.|.|| |..||| +FC12044_91407 1 TCTCCATGTTGGTCAGGCT---GGTCTC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 10/38 (26.3%) +# Similarity: 10/38 (26.3%) +# Gaps: 25/38 (65.8%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACA-----GTCCGAC------- 26 + |||.|| |||.|.| +FC12044_91407 1 -------------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 46.0 +# +# +#======================================= + +Illumina_DpnI 1 --TC---GTATG--CCGTCT--TCTGCTTG 21 + || || || |.|.|| ||| | +FC12044_91407 1 TCTCCATGT-TGGTCAGGCTGGTCT-C--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 14/50 (28.0%) +# Similarity: 14/50 (28.0%) +# Gaps: 31/50 (62.0%) +# Score: 59.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACC------GACAGGTTCAGAGTTCTACAGTCCGA 44 + . |.|| |.|||| |.|. ||| | +FC12044_91407 1 ------------T-CTCCATGTTGGTCAGG--CTGG--TCT-C------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 64.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACA-----GTCCGAC-GATC-- 32 + |||.|| |||.|.| |.|| +FC12044_91407 1 ---------------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 --TCGGAC-TGTAGAACT--CTGAAC-- 21 + || | |||.|..|. |||..| +FC12044_91407 1 TCTC---CATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 12/39 (30.8%) +# Similarity: 12/39 (30.8%) +# Gaps: 24/39 (61.5%) +# Score: 56.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACA-----GTCCGACATG----- 29 + |||.|| |||.|.| || +FC12044_91407 1 -------------TCTCCATGTTGGTCAGGC-TGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 24/36 (66.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------CAAGCAGAAGACGGCATACGANN 23 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 46.0 +# +# +#======================================= + +Illumina_NlaI 1 --TC---GTATG--CCGTCT--TCTGCTTG 21 + || || || |.|.|| ||| | +FC12044_91407 1 TCTCCATGT-TGGTCAGGCTGGTCT-C--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 14/50 (28.0%) +# Similarity: 14/50 (28.0%) +# Gaps: 31/50 (62.0%) +# Score: 59.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACC------GACAGGTTCAGAGTTCTACAGTCCGA 44 + . |.|| |.|||| |.|. ||| | +FC12044_91407 1 ------------T-CTCCATGTTGGTCAGG--CTGG--TCT-C------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 ---CC------GACAGGTTCAGAGTTCTACAGTCCGACATG 32 + || |.|||| |.|. ||| | +FC12044_91407 1 TCTCCATGTTGGTCAGG--CTGG--TCT-C----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Smal 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 13/34 (38.2%) +# Similarity: 13/34 (38.2%) +# Gaps: 17/34 (50.0%) +# Score: 64.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACA-----GTCCGAC-GATC-- 26 + |||.|| |||.|.| |.|| +FC12044_91407 1 ---------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 11/29 (37.9%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCT---TCT-GCTTGT--- 22 + || | ||.|.| ||. |||.|| +FC12044_91407 1 TC-T---CCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Smal 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 14/50 (28.0%) +# Similarity: 14/50 (28.0%) +# Gaps: 31/50 (62.0%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACC------GACAGGTTCAGAGTTCTACAGTCCGA 44 + . |.|| |.|||| |.|. ||| | +FC12044_91407 1 ------------T-CTCCATGTTGGTCAGG--CTGG--TCT-C------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 64.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACA-----GTCCGAC-GATC-- 32 + |||.|| |||.|.| |.|| +FC12044_91407 1 ---------------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 57.0 +# +# +#======================================= + +Illumina_Geno 1 -GA---TCGGAAGAGCTCGTATG--CCGTCTTCTGCTTG 33 + || ||| ||| ||.|.| |||.| +FC12044_91407 1 TGAACGTCG--AGA---CGCAAGGCCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 13/68 (19.1%) +# Similarity: 13/68 (19.1%) +# Gaps: 53/68 (77.9%) +# Score: 70.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGA----------CCACCGAGATCTACACTCTTTCCCTAC 40 + ||| |||.||| ||.|| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_Geno 41 ACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 16/38 (42.1%) +# Similarity: 16/38 (42.1%) +# Gaps: 17/38 (44.7%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAA-GACGGCATACG---AGCTCTTCCGATCT 34 + .||| |.|| |.||| .|| |||. | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGC----CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 15/36 (41.7%) +# Score: 56.0 +# +# +#======================================= + +Illumina_Pair 1 -GA---TCGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + || ||| ||| || || |||...||| +FC12044_91407 1 TGAACGTCG--AGA-CG---CA--AGGCCCGCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 13/68 (19.1%) +# Similarity: 13/68 (19.1%) +# Gaps: 53/68 (77.9%) +# Score: 70.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGA----------CCACCGAGATCTACACTCTTTCCCTAC 40 + ||| |||.||| ||.|| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_Pair 41 ACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 21/61 (34.4%) +# Similarity: 21/61 (34.4%) +# Gaps: 36/61 (59.0%) +# Score: 85.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + .|| ||| .||||| || |..||| |||| +FC12044_91407 1 -----TGA--ACG----TCGAGA-CG--CAAGGC------------CCGC 24 + +Illumina_Pair 51 TCTTCCGATCT 61 + | +FC12044_91407 25 -C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 15/45 (33.3%) +# Similarity: 15/45 (33.3%) +# Gaps: 28/45 (62.2%) +# Score: 61.0 +# +# +#======================================= + +Illumina_Pair 1 ----CGGTCTCG----GCATTCCTGCTGAACCGCTCTTCCGATCT 37 + || ||| |||. .|| |||| | +FC12044_91407 1 TGAACG---TCGAGACGCAA---GGC----CCGC-C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 13/32 (40.6%) +# Score: 62.5 +# +# +#======================================= + +Illumina_DpnI 1 -GATCGTCGGACTGTAGAACTCTGAAC----- 26 + ||.||||| || ||.|....| +FC12044_91407 1 TGAACGTCG------AG-ACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 15/31 (48.4%) +# Similarity: 15/31 (48.4%) +# Gaps: 11/31 (35.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_DpnI 1 ---ACAGGTTCAGAGTTCTACA--GTCCGAC 26 + ||. || ||| .| .|| |.|||.| +FC12044_91407 1 TGAACG---TC-GAG-AC-GCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_DpnI 1 ------TCGT-----ATG--CCGTCTTCTGCTTG 21 + |||. |.| |||.| +FC12044_91407 1 TGAACGTCGAGACGCAAGGCCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 71.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGC--GAC-CA----CCGACAGGTTCAGAGTTCTACAGTCCG 43 + ||| |||.| ||| || |||.| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 54.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAGGTTCAGAGTTCTAC---AGTCCGACGATC- 32 + ||.| ||| || ||.|| || | +FC12044_91407 1 TGAACGTC-------GAG----ACGCAAGGCC--CG--CC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 10/35 (28.6%) +# Similarity: 10/35 (28.6%) +# Gaps: 24/35 (68.6%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAAC-TCTGA-AC------------ 21 + .|||| || || || +FC12044_91407 1 ---------TGAACGTC-GAGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 15/34 (44.1%) +# Similarity: 15/34 (44.1%) +# Gaps: 14/34 (41.2%) +# Score: 48.5 +# +# +#======================================= + +Illumina_NlaI 1 ---ACAGGTTCAGAGTTCTACA--GTCCGACATG 29 + ||. || ||| .| .|| |.|||.| +FC12044_91407 1 TGAACG---TC-GAG-AC-GCAAGGCCCGCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 18/33 (54.5%) +# Score: 35.0 +# +# +#======================================= + +Illumina_NlaI 1 -------CAA---GCAGAAGACGGCATACGANN 23 + |.| ||| |.|.|.|| | +FC12044_91407 1 TGAACGTCGAGACGCA-AGGCCCGC---C---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_NlaI 1 ------TCGT-----ATG--CCGTCTTCTGCTTG 21 + |||. |.| |||.| +FC12044_91407 1 TGAACGTCGAGACGCAAGGCCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 71.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGC--GAC-CA----CCGACAGGTTCAGAGTTCTACAGTCCG 43 + ||| |||.| ||| || |||.| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 17/37 (45.9%) +# Score: 44.5 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAGGTTCAGAGTTCTACA--GTCCGACATG 32 + .||.| ||| .| .|| |.|||.| +FC12044_91407 1 TGAACGTC-------GAG-AC-GCAAGGCCCGCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 19/35 (54.3%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Smal 1 -----GTTCAGAGTTCTAC---AGTCCGACGATC- 26 + | || ||| || ||.|| || | +FC12044_91407 1 TGAACG-TC-GAG----ACGCAAGGCC--CG--CC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 23/35 (65.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_Smal 1 ------TCGT-----ATG--CCGTCTTCTGCTTGT 22 + |||. |.| |||.| +FC12044_91407 1 TGAACGTCGAGACGCAAGGCCCGCC---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 71.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGC--GAC-CA----CCGACAGGTTCAGAGTTCTACAGTCCG 43 + ||| |||.| ||| || |||.| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 54.5 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAGGTTCAGAGTTCTAC---AGTCCGACGATC- 32 + ||.| ||| || ||.|| || | +FC12044_91407 1 TGAACGTC-------GAG----ACGCAAGGCC--CG--CC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 30/44 (68.2%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Geno 1 -----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + | |||..|.|| ||| | +FC12044_91407 1 CTGTCCCCACGG--CGGGGGGGC--------------CTG---G 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 15/66 (22.7%) +# Similarity: 15/66 (22.7%) +# Gaps: 49/66 (74.2%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC----- 45 + ||.|||| ||||.| +FC12044_91407 1 ------------------------------CTGTCCC--CACGGCGGGGG 18 + +Illumina_Geno 46 -GCTCTTCCGATCT-- 58 + || || +FC12044_91407 19 GGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 12/42 (28.6%) +# Similarity: 12/42 (28.6%) +# Gaps: 25/42 (59.5%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Geno 1 ------CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-- 34 + | | |||||....|.|| || +FC12044_91407 1 CTGTCCC---C-----ACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 15/36 (41.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGT----TCAGCAGGAATGCCGAG 32 + |.|| .|.||.||...|||..| +FC12044_91407 1 -----------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 15/66 (22.7%) +# Similarity: 15/66 (22.7%) +# Gaps: 49/66 (74.2%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC----- 45 + ||.|||| ||||.| +FC12044_91407 1 ------------------------------CTGTCCC--CACGGCGGGGG 18 + +Illumina_Pair 46 -GCTCTTCCGATCT-- 58 + || || +FC12044_91407 19 GGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 67 +# Identity: 17/67 (25.4%) +# Similarity: 17/67 (25.4%) +# Gaps: 48/67 (71.6%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Pair 1 ------CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTG 44 + | | |||| ||.| || ||| ||| +FC12044_91407 1 CTGTCCC---C-----ACGG----CGGG---GG----GGC-------CTG 24 + +Illumina_Pair 45 AACCGCTCTTCCGATCT 61 + . +FC12044_91407 25 G---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 13/47 (27.7%) +# Similarity: 13/47 (27.7%) +# Gaps: 32/47 (68.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Pair 1 CGGT---CTCGGCATT-------CCTGCTGAACCGCTCTTCCGATCT 37 + |.|| |.|||| |||| | +FC12044_91407 1 CTGTCCCCACGGC---GGGGGGGCCTG--G----------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 13/32 (40.6%) +# Score: 38.0 +# +# +#======================================= + +Illumina_DpnI 1 GATC-GT-----CGGACTGTAGAACTCTGAAC 26 + | || ||| |.|..|..| |||. +FC12044_91407 1 ---CTGTCCCCACGG-CGGGGGGGC-CTGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 7/42 (16.7%) +# Similarity: 7/42 (16.7%) +# Gaps: 33/42 (78.6%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGTC----CGAC------------ 26 + |.||| ||.| +FC12044_91407 1 -----------------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_DpnI 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 29.0 +# +# +#======================================= + +Illumina_DpnI 1 ---TCGTATGCCGTCTTCTGCTTG----------- 21 + || || | .|.|| | +FC12044_91407 1 CTGTC-----CC--C-ACGGC--GGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_DpnI 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 16/47 (34.0%) +# Similarity: 16/47 (34.0%) +# Gaps: 25/47 (53.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGC-GACCACCGACAGGTTCAGAGTTCTACAGTCC--GA 44 + | |.|| || ||.| |.|.| .|.|| | +FC12044_91407 1 ----------CTGTCC-CC-ACGG---CGGGG------GGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 43.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGT-CCGACGATC------------ 32 + |.|| ||.|||. | +FC12044_91407 1 -------------------CTGTCCCCACGG-CGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 10/29 (34.5%) +# Similarity: 10/29 (34.5%) +# Gaps: 12/29 (41.4%) +# Score: 36.0 +# +# +#======================================= + +Illumina_NlaI 1 --------TCGGACTGTAGAACTCTGAAC 21 + .||| |.|..|..| |||. +FC12044_91407 1 CTGTCCCCACGG-CGGGGGGGC-CTGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 9/42 (21.4%) +# Similarity: 9/42 (21.4%) +# Gaps: 30/42 (71.4%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCC------------GACATG- 29 + |.|||| |.|.|| +FC12044_91407 1 -----------------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 14/31 (45.2%) +# Score: 32.5 +# +# +#======================================= + +Illumina_NlaI 1 -------CA-AGCAGAAGACGGCATACGANN 23 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 29.0 +# +# +#======================================= + +Illumina_NlaI 1 ---TCGTATGCCGTCTTCTGCTTG----------- 21 + || || | .|.|| | +FC12044_91407 1 CTGTC-----CC--C-ACGGC--GGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_NlaI 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 16/47 (34.0%) +# Similarity: 16/47 (34.0%) +# Gaps: 25/47 (53.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGC-GACCACCGACAGGTTCAGAGTTCTACAGTCC--GA 44 + | |.|| || ||.| |.|.| .|.|| | +FC12044_91407 1 ----------CTGTCC-CC-ACGG---CGGGG------GGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAGTCC------------GACATG- 32 + |.|||| |.|.|| +FC12044_91407 1 --------------------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_Smal 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 9/39 (23.1%) +# Similarity: 9/39 (23.1%) +# Gaps: 27/39 (69.2%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGT-CCGACGATC------------ 26 + |.|| ||.|||. | +FC12044_91407 1 -------------CTGTCCCCACGG-CGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 10/31 (32.3%) +# Similarity: 10/31 (32.3%) +# Gaps: 15/31 (48.4%) +# Score: 27.5 +# +# +#======================================= + +Illumina_Smal 1 ---TCGTATGCCGTCTTCTG------CTTGT 22 + || ||..|..|.| ||.| +FC12044_91407 1 CTGTC-----CCCACGGCGGGGGGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_Smal 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 16/47 (34.0%) +# Similarity: 16/47 (34.0%) +# Gaps: 25/47 (53.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGC-GACCACCGACAGGTTCAGAGTTCTACAGTCC--GA 44 + | |.|| || ||.| |.|.| .|.|| | +FC12044_91407 1 ----------CTGTCC-CC-ACGG---CGGGG------GGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGT-CCGACGATC------------ 32 + |.|| ||.|||. | +FC12044_91407 1 -------------------CTGTCCCCACGG-CGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 12/35 (34.3%) +# Score: 79.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGCC--GTCTTCTGCTTG 33 + ||.|..|||.||..|| |.||| || +FC12044_91407 1 -----GATGTACTCTTACACCCAGACTT-TG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 93.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + |||.|||.|| ||||| +FC12044_91407 1 --------------------GATGTACTCT-------TACAC-------- 15 + +Illumina_Geno 51 TCCGATCT--- 58 + ||...|| +FC12044_91407 16 -CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 14/41 (34.1%) +# Similarity: 14/41 (34.1%) +# Gaps: 23/41 (56.1%) +# Score: 67.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTT----CCGATCT--- 34 + ||.| || ||||| ||...|| +FC12044_91407 1 ------GATG------TA----CTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 12/39 (30.8%) +# Similarity: 12/39 (30.8%) +# Gaps: 21/39 (53.8%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTC-AGCAGGAATGCCGAG------ 32 + ||.|..| || ..|| ||.|| +FC12044_91407 1 -----GATGTAC---TCTTACA------CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 93.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + |||.|||.|| ||||| +FC12044_91407 1 --------------------GATGTACTCT-------TACAC-------- 15 + +Illumina_Pair 51 TCCGATCT--- 58 + ||...|| +FC12044_91407 16 -CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 65 +# Identity: 19/65 (29.2%) +# Similarity: 19/65 (29.2%) +# Gaps: 44/65 (67.7%) +# Score: 69.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGT-CTCGGCATTCCTGCTGAACCG 49 + ||| || ||| ||.| ||| +FC12044_91407 1 ---------------------GAT--GTACTC----TTAC------ACC- 16 + +Illumina_Pair 50 CTCTTCCGATCT--- 61 + |.|| || +FC12044_91407 17 -----CAGA-CTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 13/44 (29.5%) +# Similarity: 13/44 (29.5%) +# Gaps: 26/44 (59.1%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGCTCTT----CCGATCT--- 37 + |.||.| ||||| ||...|| +FC12044_91407 1 ----------------GATGTA---CTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 13/37 (35.1%) +# Similarity: 13/37 (35.1%) +# Gaps: 23/37 (62.2%) +# Score: 57.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGA-AC---------- 26 + || ||| |||||.| || +FC12044_91407 1 --------GA-TGT---ACTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 21/36 (58.3%) +# Score: 63.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGA-GTTC---TACAGTCC--GAC---- 26 + || ||.| |||| || ||| +FC12044_91407 1 ---------GATGTACTCTTACA--CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 55.5 +# +# +#======================================= + +Illumina_DpnI 1 -------TCGTATGCC--GTCTTCTGCTTG 21 + ||.||..|| |.||| || +FC12044_91407 1 GATGTACTCTTACACCCAGACTT-TG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGAC---CACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + .||| ||| .| |||| ||||.|| |. +FC12044_91407 1 GATG-TAC----TCTTACACC--------CAGACTT-TG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 58.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGA-GTTC---TACAGTCC--GAC---GATC 32 + || ||.| |||| || ||| | +FC12044_91407 1 -----------GATGTACTCTTACA--CCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 18/32 (56.2%) +# Score: 57.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCTGA-AC---------- 21 + || ||| |||||.| || +FC12044_91407 1 ---GA-TGT---ACTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 18/36 (50.0%) +# Score: 65.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGA-GTTC---TACAGTCC--GAC-ATG 29 + || ||.| |||| || ||| .|| +FC12044_91407 1 ---------GATGTACTCTTACA--CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 10/35 (28.6%) +# Similarity: 10/35 (28.6%) +# Gaps: 22/35 (62.9%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCATAC-----------GANN 23 + ||.| || .|.||| | +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 55.5 +# +# +#======================================= + +Illumina_NlaI 1 -------TCGTATGCC--GTCTTCTGCTTG 21 + ||.||..|| |.||| || +FC12044_91407 1 GATGTACTCTTACACCCAGACTT-TG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGAC---CACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + .||| ||| .| |||| ||||.|| |. +FC12044_91407 1 GATG-TAC----TCTTACACC--------CAGACTT-TG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 65.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGA-GTTC---TACAGTCC--GAC-ATG 32 + || ||.| |||| || ||| .|| +FC12044_91407 1 ------------GATGTACTCTTACA--CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 19/35 (54.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Smal 1 ---GTTCAGAGTTC-TACAGTCC--GAC---GATC 26 + ||.| || |||| || ||| | +FC12044_91407 1 GATGTAC-----TCTTACA--CCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 15/31 (48.4%) +# Score: 55.5 +# +# +#======================================= + +Illumina_Smal 1 -------TCGTATGCC--GTCTTCTGCTTGT 22 + ||.||..|| |.||| || +FC12044_91407 1 GATGTACTCTTACACCCAGACTT-TG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGAC---CACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + .||| ||| .| |||| ||||.|| |. +FC12044_91407 1 GATG-TAC----TCTTACACC--------CAGACTT-TG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGA-GTTC---TACAGTCC--GAC---GATC 32 + || ||.| |||| || ||| | +FC12044_91407 1 -----------GATGTACTCTTACA--CCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 14/42 (33.3%) +# Similarity: 14/42 (33.3%) +# Gaps: 26/42 (61.9%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Geno 1 GATC---GGAAGA-----GCTCGTATGCC-GTCTTCTGCTTG 33 + || ||..|| |||| || || +FC12044_91407 1 --TCAAGGGTGGATCTTGGCTC-----CCAGT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Geno 1 --AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT 48 + ||.|.| .||||| |. ||| +FC12044_91407 1 TCAAGGGT-------------GGATCT------------TG------GCT 19 + +Illumina_Geno 49 CTTCCGATCT 58 + | ||..| +FC12044_91407 20 C--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 11/35 (31.4%) +# Score: 63.0 +# +# +#======================================= + +Illumina_Geno 1 -CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + |||| .|..|| .|.| .|||| ||..| +FC12044_91407 1 TCAAG-GGTGGA--TCTT---GGCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 13/35 (37.1%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCA--GCAGGAATGCCGAG- 32 + || |||.|.||.||. ||. ||.|| +FC12044_91407 1 --TC--AAGGGTGGATCTTGGCT------CCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Pair 1 --AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT 48 + ||.|.| .||||| |. ||| +FC12044_91407 1 TCAAGGGT-------------GGATCT------------TG------GCT 19 + +Illumina_Pair 49 CTTCCGATCT 58 + | ||..| +FC12044_91407 20 C--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 18/62 (29.0%) +# Similarity: 18/62 (29.0%) +# Gaps: 38/62 (61.3%) +# Score: 74.0 +# +# +#======================================= + +Illumina_Pair 1 -CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG 49 + |||| |.|.|.|||.||| ||| |.|. +FC12044_91407 1 TCAAG-----------------GGTGGATCTTGGC--TCC--CAGT---- 25 + +Illumina_Pair 50 CTCTTCCGATCT 61 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 19/41 (46.3%) +# Similarity: 19/41 (46.3%) +# Gaps: 20/41 (48.8%) +# Score: 65.0 +# +# +#======================================= + +Illumina_Pair 1 -C---GGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + | ||| || || || || |||| ||..| +FC12044_91407 1 TCAAGGGT---GG-AT--CT--TG----GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTC--GGACTGTAGAACT---CTGAAC----- 26 + || || ||.||.|| || | +FC12044_91407 1 -----TCAAGG---GTGGATCTTGGCT---CCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 58.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTC-AGAGT---TCTA------CAGTCCGAC 26 + || ||.|| |||. |||| +FC12044_91407 1 ------TCAAGGGTGGATCTTGGCTCCCAGT----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_DpnI 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 38.5 +# +# +#======================================= + +Illumina_DpnI 1 TC--GTATGCCG-TCTT--CTGCTTG- 21 + || |..|| | |||| ||.|..| +FC12044_91407 1 TCAAGGGTG--GATCTTGGCTCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_DpnI 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 13/54 (24.1%) +# Similarity: 13/54 (24.1%) +# Gaps: 39/54 (72.2%) +# Score: 58.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCTA------CAGT 40 + || ||.|| |||. |||| +FC12044_91407 1 -------------------------TCAAGGGTGGATCTTGGCTCCCAGT 25 + +Illumina_DpnI 41 CCGA 44 + +FC12044_91407 26 ---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 60.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTC-AGAGT---TCTA--CAGTCCGACGATC 32 + || ||.|| |||. | ||| |..| +FC12044_91407 1 --------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 13/31 (41.9%) +# Similarity: 13/31 (41.9%) +# Gaps: 16/31 (51.6%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 TC--GGACTGTAGAACT---CTGAAC----- 21 + || || ||.||.|| || | +FC12044_91407 1 TCAAGG---GTGGATCTTGGCT---CCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 18/36 (50.0%) +# Score: 60.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTC-AGAGT---TCTA--CAGTCCGACA-TG 29 + || ||.|| |||. | ||| || | +FC12044_91407 1 ------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 10/29 (34.5%) +# Score: 38.0 +# +# +#======================================= + +Illumina_NlaI 1 -CAAGCAGAAGA-C--GGCATAC--GANN 23 + |||| .|..|| | ||| |.| |. +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 38.5 +# +# +#======================================= + +Illumina_NlaI 1 TC--GTATGCCG-TCTT--CTGCTTG- 21 + || |..|| | |||| ||.|..| +FC12044_91407 1 TCAAGGGTG--GATCTTGGCTCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_NlaI 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 13/54 (24.1%) +# Similarity: 13/54 (24.1%) +# Gaps: 39/54 (72.2%) +# Score: 58.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCTA------CAGT 40 + || ||.|| |||. |||| +FC12044_91407 1 -------------------------TCAAGGGTGGATCTTGGCTCCCAGT 25 + +Illumina_NlaI 41 CCGA 44 + +FC12044_91407 26 ---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 60.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTC-AGAGT---TCTA--CAGTCCGACA-TG 32 + || ||.|| |||. | ||| || | +FC12044_91407 1 ---------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Smal 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 15/32 (46.9%) +# Similarity: 15/32 (46.9%) +# Gaps: 13/32 (40.6%) +# Score: 60.5 +# +# +#======================================= + +Illumina_Smal 1 GTTC-AGAGT---TCTA--CAGTCCGACGATC 26 + || ||.|| |||. | ||| |..| +FC12044_91407 1 --TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 15/27 (55.6%) +# Similarity: 15/27 (55.6%) +# Gaps: 7/27 (25.9%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Smal 1 TC--GTATGCCG-TCTT--CTGCTTGT 22 + || |..|| | |||| ||.|..|| +FC12044_91407 1 TCAAGGGTG--GATCTTGGCTCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Smal 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 13/54 (24.1%) +# Similarity: 13/54 (24.1%) +# Gaps: 39/54 (72.2%) +# Score: 58.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCTA------CAGT 40 + || ||.|| |||. |||| +FC12044_91407 1 -------------------------TCAAGGGTGGATCTTGGCTCCCAGT 25 + +Illumina_Smal 41 CCGA 44 + +FC12044_91407 26 ---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 60.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTC-AGAGT---TCTA--CAGTCCGACGATC 32 + || ||.|| |||. | ||| |..| +FC12044_91407 1 --------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 18/38 (47.4%) +# Score: 63.5 +# +# +#======================================= + +Illumina_Geno 1 GAT--CGGAAGAGCTCGTATGCCGTCTT---CTGCTTG 33 + | ||..|||| | |.||| |||| | +FC12044_91407 1 --TTGCGACAGAG-T--TTTGC--TCTTGTCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 59 +# Identity: 20/59 (33.9%) +# Similarity: 20/59 (33.9%) +# Gaps: 35/59 (59.3%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTT-TCCCTACACGACGCTC 49 + |. |||| || |||.|.| .||||| ||| +FC12044_91407 1 -----TT--GCGA-CA--GAGTTTT--GCTCTTGTCC------------- 25 + +Illumina_Geno 50 TTCCGATCT 58 + +FC12044_91407 26 --------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 23/41 (56.1%) +# Score: 69.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGA-----GCTCT--TCCGATCT 34 + ..| ||.|| || ||||| ||| +FC12044_91407 1 -------TTG-CGACA---GAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 13/35 (37.1%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Pair 1 GAT--CGGAAGAGCGGTTCAGCAGGAATG-CCGAG 32 + | ||..||| |||..||. ..|| || +FC12044_91407 1 --TTGCGACAGA---GTTTTGCT--CTTGTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 59 +# Identity: 20/59 (33.9%) +# Similarity: 20/59 (33.9%) +# Gaps: 35/59 (59.3%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTT-TCCCTACACGACGCTC 49 + |. |||| || |||.|.| .||||| ||| +FC12044_91407 1 -----TT--GCGA-CA--GAGTTTT--GCTCTTGTCC------------- 25 + +Illumina_Pair 50 TTCCGATCT 58 + +FC12044_91407 26 --------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 63 +# Identity: 19/63 (30.2%) +# Similarity: 19/63 (30.2%) +# Gaps: 40/63 (63.5%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + ..| ||.|| |||.| || || +FC12044_91407 1 -------TTG-CGACA---GAGTT-----------TT-----------GC 17 + +Illumina_Pair 51 TCT--TCCGATCT 61 + ||| ||| +FC12044_91407 18 TCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 15/46 (32.6%) +# Similarity: 15/46 (32.6%) +# Gaps: 30/46 (65.2%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCT--CGGCA-----TTCCTGCTGAACCGCTCT--TCCGATCT 37 + | ||.|| || ||||| ||| +FC12044_91407 1 -----TTGCGACAGAGTTTT-----------GCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 7/29 (24.1%) +# Score: 60.0 +# +# +#======================================= + +Illumina_DpnI 1 GAT--CGTCGGACTGTAGAACTCTGAAC- 26 + | ||.|.||.|.|.| ||||...| +FC12044_91407 1 --TTGCGACAGAGTTTTG--CTCTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 15/33 (45.5%) +# Similarity: 15/33 (45.5%) +# Gaps: 15/33 (45.5%) +# Score: 78.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTT----CAGAGTTCTAC---AGTCCGAC 26 + || |||||||.|.| .|||| +FC12044_91407 1 -----TTGCGACAGAGTTTTGCTCTTGTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_DpnI 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 56.5 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGC--C---GTCTT-CTGCTTG--- 21 + .||| | ||.|| || |||| +FC12044_91407 1 ----TTGCGACAGAGTTTTGCT-CTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_DpnI 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 83.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTAC---AGTCCGA 44 + |. |||| |||||||.|.| .|||| +FC12044_91407 1 -----TT--GCGA-------------CAGAGTTTTGCTCTTGTCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 17/39 (43.6%) +# Similarity: 17/39 (43.6%) +# Gaps: 21/39 (53.8%) +# Score: 86.5 +# +# +#======================================= + +Illumina_DpnI 1 ---CGACAG-GTT---CAGAGTTCTACAGTCCGACGATC 32 + |||||| ||| | ||| .|||| +FC12044_91407 1 TTGCGACAGAGTTTTGC-----TCT--TGTCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 13/28 (46.4%) +# Similarity: 13/28 (46.4%) +# Gaps: 10/28 (35.7%) +# Score: 49.0 +# +# +#======================================= + +Illumina_NlaI 1 T--CGGACTG----TAGAACTCTGAAC- 21 + | | |||.| |.| ||||...| +FC12044_91407 1 TTGC-GACAGAGTTTTG--CTCTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 15/36 (41.7%) +# Similarity: 15/36 (41.7%) +# Gaps: 18/36 (50.0%) +# Score: 78.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTT----CAGAGTTCTAC---AGTCCGACATG 29 + || |||||||.|.| .|||| +FC12044_91407 1 -----TTGCGACAGAGTTTTGCTCTTGTCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 9/36 (25.0%) +# Similarity: 9/36 (25.0%) +# Gaps: 24/36 (66.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CAAGCAGA----------AGACGGCATACGANN 23 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGC--C---GTCTT-CTGCTTG--- 21 + .||| | ||.|| || |||| +FC12044_91407 1 ----TTGCGACAGAGTTTTGCT-CTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 83.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTAC---AGTCCGA 44 + |. |||| |||||||.|.| .|||| +FC12044_91407 1 -----TT--GCGA-------------CAGAGTTTTGCTCTTGTCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 21/39 (53.8%) +# Score: 78.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTT----CAGAGTTCTAC---AGTCCGACATG 32 + || |||||||.|.| .|||| +FC12044_91407 1 --------TTGCGACAGAGTTTTGCTCTTGTCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_Smal 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 15/33 (45.5%) +# Similarity: 15/33 (45.5%) +# Gaps: 15/33 (45.5%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Smal 1 GTT----CAGAGTTCTAC---AGTCCGACGATC 26 + || |||||||.|.| .|||| +FC12044_91407 1 -TTGCGACAGAGTTTTGCTCTTGTCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 15/30 (50.0%) +# Similarity: 15/30 (50.0%) +# Gaps: 13/30 (43.3%) +# Score: 63.5 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGC--C---GTCTT-CTGCTTGT-- 22 + .||| | ||.|| || ||||| +FC12044_91407 1 ----TTGCGACAGAGTTTTGCT-CTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_Smal 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 83.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTAC---AGTCCGA 44 + |. |||| |||||||.|.| .|||| +FC12044_91407 1 -----TT--GCGA-------------CAGAGTTTTGCTCTTGTCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 17/39 (43.6%) +# Similarity: 17/39 (43.6%) +# Gaps: 21/39 (53.8%) +# Score: 86.5 +# +# +#======================================= + +Illumina_Smal 1 ---CGACAG-GTT---CAGAGTTCTACAGTCCGACGATC 32 + |||||| ||| | ||| .|||| +FC12044_91407 1 TTGCGACAGAGTTTTGC-----TCT--TGTCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 22/40 (55.0%) +# Score: 55.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAG----AGCTC---GTATGCCGTCTTCTGCTTG 33 + || .| ||||| | |.||||.| +FC12044_91407 1 --TC---TGCTCCAGCTCCAAG-ACGCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 19/60 (31.7%) +# Similarity: 19/60 (31.7%) +# Gaps: 37/60 (61.7%) +# Score: 99.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCC--CTACACGACGCT 48 + |||.| ||| ||.||.||||| +FC12044_91407 1 ----------------------TCTGC------TCCAGCTCCAAGACGC- 21 + +Illumina_Geno 49 CTTCCGATCT 58 + | .|| +FC12044_91407 22 C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 15/44 (34.1%) +# Similarity: 15/44 (34.1%) +# Gaps: 29/44 (65.9%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 ------CAAGCAG----AAGACGGCATACGAGCTCTTCCGATCT 34 + | ||| ||||| || || || +FC12044_91407 1 TCTGCTC---CAGCTCCAAGAC-GC---CG-------CC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 57.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-------- 32 + || .|| |.||||. ||.|| +FC12044_91407 1 --TC-----TGC--TCCAGCT------CCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 19/60 (31.7%) +# Similarity: 19/60 (31.7%) +# Gaps: 37/60 (61.7%) +# Score: 99.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCC--CTACACGACGCT 48 + |||.| ||| ||.||.||||| +FC12044_91407 1 ----------------------TCTGC------TCCAGCTCCAAGACGC- 21 + +Illumina_Pair 49 CTTCCGATCT 58 + | .|| +FC12044_91407 22 C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 18/66 (27.3%) +# Similarity: 18/66 (27.3%) +# Gaps: 46/66 (69.7%) +# Score: 92.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCT----GA- 45 + ||| || |||.||| || +FC12044_91407 1 ---------------------------TCT--GC--TCCAGCTCCAAGAC 19 + +Illumina_Pair 46 ACCGCTCTTCCGATCT 61 + .|||| | +FC12044_91407 20 GCCGC-C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 18/42 (42.9%) +# Similarity: 18/42 (42.9%) +# Gaps: 22/42 (52.4%) +# Score: 92.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCT----GA-ACCGCTCTTCCGATCT 37 + ||| || |||.||| || .|||| | +FC12044_91407 1 ---TCT--GC--TCCAGCTCCAAGACGCCGC-C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 19/35 (54.3%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 GATC-G-TCGGACTGTAGAACTCTGA-AC------ 26 + || | || |.| |||..| || +FC12044_91407 1 --TCTGCTC---CAG-----CTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 14/35 (40.0%) +# Similarity: 14/35 (40.0%) +# Gaps: 19/35 (54.3%) +# Score: 72.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAG-TCC--GAC------ 26 + ||.| ||.||| ||| ||| +FC12044_91407 1 ------TCTG----CTCCAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 51.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTCTTCTGCT---TG-------- 21 + || ||| || |.||| .| +FC12044_91407 1 TC---TGC--TC--CAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 12/54 (22.2%) +# Similarity: 12/54 (22.2%) +# Gaps: 39/54 (72.2%) +# Score: 64.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA----- 44 + ||.| ||.||| |||.| +FC12044_91407 1 -------------------------TCTG----CTCCAGCTCCAAGACGC 21 + +Illumina_DpnI 45 ---- 44 + +FC12044_91407 22 CGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 77.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAG-TCC--GACGATC---- 32 + ||.| ||.||| ||| |||| | +FC12044_91407 1 --------TCTG----CTCCAGCTCCAAGACG--CCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTG-T--AGAACTCTGA-AC------ 21 + .||| | || |||..| || +FC12044_91407 1 ----TCTGCTCCAG--CTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 20/37 (54.1%) +# Score: 67.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAG-TCC--GACATG----- 29 + ||.| ||.||| ||| ||| | +FC12044_91407 1 ------TCTG----CTCCAGCTCCAAGAC--GCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 22/35 (62.9%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAG----AAGACG--GCATACGANN 23 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTCTTCTGCT---TG-------- 21 + || ||| || |.||| .| +FC12044_91407 1 TC---TGC--TC--CAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 12/54 (22.2%) +# Similarity: 12/54 (22.2%) +# Gaps: 39/54 (72.2%) +# Score: 64.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA----- 44 + ||.| ||.||| |||.| +FC12044_91407 1 -------------------------TCTG----CTCCAGCTCCAAGACGC 21 + +Illumina_NlaI 45 ---- 44 + +FC12044_91407 22 CGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 67.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAG-TCC--GACATG----- 32 + ||.| ||.||| ||| ||| | +FC12044_91407 1 ---------TCTG----CTCCAGCTCCAAGAC--GCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 16/33 (48.5%) +# Similarity: 16/33 (48.5%) +# Gaps: 15/33 (45.5%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAG-TCC--GACGATC---- 26 + ||.| ||.||| ||| |||| | +FC12044_91407 1 --TCTG----CTCCAGCTCCAAGACG--CCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 17/32 (53.1%) +# Score: 50.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCTTCTGCT---TGT------- 22 + || ||| || |.||| .|. +FC12044_91407 1 TC---TGC--TC--CAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 12/54 (22.2%) +# Similarity: 12/54 (22.2%) +# Gaps: 39/54 (72.2%) +# Score: 64.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA----- 44 + ||.| ||.||| |||.| +FC12044_91407 1 -------------------------TCTG----CTCCAGCTCCAAGACGC 21 + +Illumina_Smal 45 ---- 44 + +FC12044_91407 22 CGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAG-TCC--GACGATC---- 32 + ||.| ||.||| ||| |||| | +FC12044_91407 1 --------TCTG----CTCCAGCTCCAAGACG--CCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 12/37 (32.4%) +# Similarity: 12/37 (32.4%) +# Gaps: 16/37 (43.2%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAA----GAGCTCGTATGCCGTCTTCTGCTTG 33 + ..||||. |.||..|.|.|.|| +FC12044_91407 1 -TACGGAGCCGCGGGCGGGAAAGGCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 69.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCAC---CGAGATCTACACTCTTTCCCTACACGACGC 47 + |||||.| ||.| ||.|| |.|.|| +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGGA------------------AAGGCG- 25 + +Illumina_Geno 48 TCTTCCGATCT 58 + +FC12044_91407 26 ----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 9/46 (19.6%) +# Similarity: 9/46 (19.6%) +# Gaps: 33/46 (71.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Geno 1 ----------CAAGCAG--AAGACGGCATACGAGCTCTTCCGATCT 34 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG--------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 17/32 (53.1%) +# Similarity: 17/32 (53.1%) +# Gaps: 7/32 (21.9%) +# Score: 75.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + ..||||...|||| ||.||||.|.|| +FC12044_91407 1 -TACGGAGCCGCGG----GCGGGAAAGGCG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 69.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCAC---CGAGATCTACACTCTTTCCCTACACGACGC 47 + |||||.| ||.| ||.|| |.|.|| +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGGA------------------AAGGCG- 25 + +Illumina_Pair 48 TCTTCCGATCT 58 + +FC12044_91407 26 ----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 17/64 (26.6%) +# Similarity: 17/64 (26.6%) +# Gaps: 42/64 (65.6%) +# Score: 56.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAAC--- 47 + |||| ||.| |.||| ||.|.|. +FC12044_91407 1 ----------------TACG-GAGC---CGCGG-------GCGGGAAAGG 23 + +Illumina_Pair 48 CGCTCTTCCGATCT 61 + || +FC12044_91407 24 CG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 11/48 (22.9%) +# Similarity: 11/48 (22.9%) +# Gaps: 34/48 (70.8%) +# Score: 45.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGCTCTT---C--------CGATCT 37 + |.|.||.|||| | || +FC12044_91407 1 ---------------TACGGAGCCGC----GGGCGGGAAAGGCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 19/35 (54.3%) +# Score: 35.0 +# +# +#======================================= + +Illumina_DpnI 1 ----GAT-CGTCGGACTGTAGAACTCTGAA---C- 26 + ||. || |||.|.| ||| | +FC12044_91407 1 TACGGAGCCG-CGGGCGG---------GAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 9/40 (22.5%) +# Similarity: 9/40 (22.5%) +# Gaps: 29/40 (72.5%) +# Score: 44.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGTCCGAC-------------- 26 + |.|.||| ||| | +FC12044_91407 1 -----TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 31.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATGCCGTCTTC-TGCTTG--------- 21 + .||.| |||| | .|| | +FC12044_91407 1 TACGGA-GCCG----CGGGC--GGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 19/44 (43.2%) +# Similarity: 19/44 (43.2%) +# Gaps: 19/44 (43.2%) +# Score: 71.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + |||||.| ||.|.|.|.|| |.|| | || +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGG---AAAG-------G--CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 11/42 (26.2%) +# Similarity: 11/42 (26.2%) +# Gaps: 27/42 (64.3%) +# Score: 52.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGTCCGACGATC---------- 32 + |.|.||| ||| ||..| +FC12044_91407 1 -------TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGGA-CTGTAGAACTCTGAAC---------- 21 + .|||| | |.| |..| +FC12044_91407 1 TACGGAGC-------CGC-GGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 10/40 (25.0%) +# Similarity: 10/40 (25.0%) +# Gaps: 26/40 (65.0%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCCGACATG----------- 29 + |.|.||| ||| |..| +FC12044_91407 1 -----TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 22/35 (62.9%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAG--AAGACGGCATACGANN 23 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATGCCGTCTTC-TGCTTG--------- 21 + .||.| |||| | .|| | +FC12044_91407 1 TACGGA-GCCG----CGGGC--GGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 19/44 (43.2%) +# Similarity: 19/44 (43.2%) +# Gaps: 19/44 (43.2%) +# Score: 71.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + |||||.| ||.|.|.|.|| |.|| | || +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGG---AAAG-------G--CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 10/43 (23.3%) +# Similarity: 10/43 (23.3%) +# Gaps: 29/43 (67.4%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAGTCCGACATG----------- 32 + |.|.||| ||| |..| +FC12044_91407 1 --------TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 11/36 (30.6%) +# Similarity: 11/36 (30.6%) +# Gaps: 21/36 (58.3%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGTCCGACGATC---------- 26 + |.|.||| ||| ||..| +FC12044_91407 1 -TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 11/30 (36.7%) +# Similarity: 11/30 (36.7%) +# Gaps: 13/30 (43.3%) +# Score: 28.5 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATGCCGTCTTC-TGCTTGT------ 22 + .||.| |||| | .||..|. +FC12044_91407 1 TACGGA-GCCG----CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 19/44 (43.2%) +# Similarity: 19/44 (43.2%) +# Gaps: 19/44 (43.2%) +# Score: 71.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + |||||.| ||.|.|.|.|| |.|| | || +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGG---AAAG-------G--CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 11/42 (26.2%) +# Similarity: 11/42 (26.2%) +# Gaps: 27/42 (64.3%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGTCCGACGATC---------- 32 + |.|.||| ||| ||..| +FC12044_91407 1 -------TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Geno 1 --------GATCGGAAGAGCTCG--TATGCCGTCTTCTGCTTG 33 + |.|| ||| || ||| || | +FC12044_91407 1 CCTCCCAGGTTC--AAG----CGATTAT-CC-T---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 18/64 (28.1%) +# Similarity: 18/64 (28.1%) +# Gaps: 45/64 (70.3%) +# Score: 73.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TACA--CGAC 45 + || |||| |.|| ||| +FC12044_91407 1 ----------------CC---------------TCCCAGGTTCAAGCGA- 18 + +Illumina_Geno 46 GCTCTTCCGAT-CT 58 + || || || +FC12044_91407 19 ----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 13/46 (28.3%) +# Similarity: 13/46 (28.3%) +# Gaps: 33/46 (71.7%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTC-----TTC---CGAT----CT 34 + | ||| ||| |||| || +FC12044_91407 1 ------------------C---CTCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 55.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGC------GGTTC-AGCAGGAATG-CCGAG 32 + | ||||| ||| ||.|. ||. +FC12044_91407 1 -----------CCTCCCAGGTTCAAGC--GATTATCCT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 18/64 (28.1%) +# Similarity: 18/64 (28.1%) +# Gaps: 45/64 (70.3%) +# Score: 73.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TACA--CGAC 45 + || |||| |.|| ||| +FC12044_91407 1 ----------------CC---------------TCCCAGGTTCAAGCGA- 18 + +Illumina_Pair 46 GCTCTTCCGAT-CT 58 + || || || +FC12044_91407 19 ----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 71 +# Identity: 15/71 (21.1%) +# Similarity: 15/71 (21.1%) +# Gaps: 56/71 (78.9%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + ||| || +FC12044_91407 1 -------------------------------------CCT------CC-- 5 + +Illumina_Pair 51 TC---TTC---CGAT----CT 61 + | ||| |||| || +FC12044_91407 6 -CAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 32/47 (68.1%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGCTC---TTC---CGAT----CT 37 + ||| || | ||| |||| || +FC12044_91407 1 -------------CCT------CC---CAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 7/42 (16.7%) +# Similarity: 7/42 (16.7%) +# Gaps: 33/42 (78.6%) +# Score: 32.0 +# +# +#======================================= + +Illumina_DpnI 1 ----------------GATCGTCGGACTGTAGAACTCTGAAC 26 + |||..|| || +FC12044_91407 1 CCTCCCAGGTTCAAGCGATTATC---CT-------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 16/31 (51.6%) +# Similarity: 16/31 (51.6%) +# Gaps: 11/31 (35.5%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 ----ACAGGTTC-AGAGTTCTACAGTCCGAC 26 + .||||||| ||.|.| || |||. +FC12044_91407 1 CCTCCCAGGTTCAAGCGAT-TA---TCCT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 20/33 (60.6%) +# Score: 45.5 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTC-----TTC-TGC--TTG---- 21 + || || ||| .|| ||. +FC12044_91407 1 -------CC-TCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 101.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTTCTACAGTCCGA 44 + ||.|| ||||||| ||.|.| || |||. +FC12044_91407 1 -------------CCTCC--CAGGTTCAAGCGAT-TA---TCCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 19/38 (50.0%) +# Score: 76.0 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAGGTTCAGAGTTCTACAGTCCGACGATC-- 32 + | |||||||| || |||..||| +FC12044_91407 1 CCTCC--CAGGTTCA-AG----------CGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 8/31 (25.8%) +# Similarity: 8/31 (25.8%) +# Gaps: 16/31 (51.6%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 ----------TCGGACTGTAGAACTCTGAAC 21 + ||...| |...|.| || +FC12044_91407 1 CCTCCCAGGTTCAAGC-GATTATC-CT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 14/34 (41.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_NlaI 1 ----ACAGGTTC-AGAGTTCTACAGTCCGACATG 29 + .||||||| ||.|.| || ||| | +FC12044_91407 1 CCTCCCAGGTTCAAGCGAT-TA---TCC----T- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 7/34 (20.6%) +# Similarity: 7/34 (20.6%) +# Gaps: 20/34 (58.8%) +# Score: 37.5 +# +# +#======================================= + +Illumina_NlaI 1 -----------CAAGCAGAAGACGGCATACGANN 23 + |||||......| |. +FC12044_91407 1 CCTCCCAGGTTCAAGCGATTATC--CT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 20/33 (60.6%) +# Score: 45.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTC-----TTC-TGC--TTG---- 21 + || || ||| .|| ||. +FC12044_91407 1 -------CC-TCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 101.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTTCTACAGTCCGA 44 + ||.|| ||||||| ||.|.| || |||. +FC12044_91407 1 -------------CCTCC--CAGGTTCAAGCGAT-TA---TCCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 19/36 (52.8%) +# Similarity: 19/36 (52.8%) +# Gaps: 15/36 (41.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAGGTTC-AGAGTTCTACAGTCCGACATG 32 + || ||||||| ||.|.| || ||| | +FC12044_91407 1 CCTCC--CAGGTTCAAGCGAT-TA---TCC----T- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 54.5 +# +# +#======================================= + +Illumina_Smal 1 --------GTTCAGAGTTCTACAGTCCGACGATC-- 26 + ||||| || |||..||| +FC12044_91407 1 CCTCCCAGGTTCA-AG----------CGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 19/33 (57.6%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTC-----TTC-TGC--TTGT--- 22 + || || ||| .|| ||.| +FC12044_91407 1 -------CC-TCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 101.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTTCTACAGTCCGA 44 + ||.|| ||||||| ||.|.| || |||. +FC12044_91407 1 -------------CCTCC--CAGGTTCAAGCGAT-TA---TCCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 19/38 (50.0%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAGGTTCAGAGTTCTACAGTCCGACGATC-- 32 + | |||||||| || |||..||| +FC12044_91407 1 CCTCC--CAGGTTCA-AG----------CGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 12/43 (27.9%) +# Similarity: 12/43 (27.9%) +# Gaps: 28/43 (65.1%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGT-ATGCCGTCTTCTGCTTG--------- 33 + || |||.||.| |.|| | +FC12044_91407 1 --------------GTCATGGCGGC--CCGC--GCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Geno 1 --AATGATACGGCGACCAC-CGAGATCTACACTCTTTCCCTACACGA-CG 46 + .||| .|||| ||.| ||.| || || +FC12044_91407 1 GTCATG--GCGGC--CCGCGCGGG---------------------GAGCG 25 + +Illumina_Geno 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 29/44 (65.9%) +# Score: 46.0 +# +# +#======================================= + +Illumina_Geno 1 --CAAGCAGAAGACGGCATAC--------GAGCTCTTCCGATCT 34 + | |.|.|||| | ||||. +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 17/35 (48.6%) +# Similarity: 17/35 (48.6%) +# Gaps: 13/35 (37.1%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTC---AGCAGGAATGCCGAG 32 + .|| |.| |||| | .||.||.| | || +FC12044_91407 1 -GTC--ATG-GCGG--CCCGCGCGGGGA-G-CG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Pair 1 --AATGATACGGCGACCAC-CGAGATCTACACTCTTTCCCTACACGA-CG 46 + .||| .|||| ||.| ||.| || || +FC12044_91407 1 GTCATG--GCGGC--CCGCGCGGG---------------------GAGCG 25 + +Illumina_Pair 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 14/70 (20.0%) +# Similarity: 14/70 (20.0%) +# Gaps: 54/70 (77.1%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCT---CGGCATTCCTGCTGAAC 47 + |||. |||| | +FC12044_91407 1 --------------------------GTCATGGCGGC------------C 12 + +Illumina_Pair 48 CGCTCTTC------CGATCT 61 + ||| .| || +FC12044_91407 13 CGC---GCGGGGAGCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 14/46 (30.4%) +# Similarity: 14/46 (30.4%) +# Gaps: 30/46 (65.2%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCT---CGGCATTCCTGCTGAACCGCTCTTC------CGATCT 37 + |||. |||| |||| .| || +FC12044_91407 1 --GTCATGGCGGC------------CCGC---GCGGGGAGCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 15/33 (45.5%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGT--CGGACTGTAGAACTC----TGAAC- 26 + .||.| |||.| |.| .||.| +FC12044_91407 1 -GTCATGGCGGCC-------CGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 11/36 (30.6%) +# Similarity: 11/36 (30.6%) +# Gaps: 21/36 (58.3%) +# Score: 37.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGTCCGAC---------- 26 + | |||..| |.|.||| | +FC12044_91407 1 ---G--TCATGG-----CGGCCCG-CGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_DpnI 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATGCCGTCTTCTGCTTG--------- 21 + || |||.||.| |.|| | +FC12044_91407 1 GTC--ATGGCGGC--CCGC--GCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_DpnI 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCAC-CGACAGGTTCAGAGTTCTACAGTCCGA 44 + .|.|| ||||.|| | || |.|| ||| || +FC12044_91407 1 -GTCAT--GGCGGCC-CGCG-CGGG----GAG----------CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 45.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACA-GGTTCAGAGTTCTACAGTCCGACGATC-------- 32 + |.|| || |.|.||| || | +FC12044_91407 1 -GTCATGG------------CGGCCCG-CG--CGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 26.0 +# +# +#======================================= + +Illumina_NlaI 1 ----T--CGGACTGTAGAACTC----TGAAC- 21 + | |||.| |.| .||.| +FC12044_91407 1 GTCATGGCGGCC-------CGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 5/48 (10.4%) +# Similarity: 5/48 (10.4%) +# Gaps: 42/48 (87.5%) +# Score: 34.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCCGACATG------------------- 29 + |.|||| +FC12044_91407 1 -----------------------GTCATGGCGGCCCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 20/34 (58.8%) +# Score: 35.5 +# +# +#======================================= + +Illumina_NlaI 1 --CAAGCAGAAGACGGC---------ATACGANN 23 + | |.|.|||| ...|| +FC12044_91407 1 GTC------ATGGCGGCCCGCGCGGGGAGCG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATGCCGTCTTCTGCTTG--------- 21 + || |||.||.| |.|| | +FC12044_91407 1 GTC--ATGGCGGC--CCGC--GCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_NlaI 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCAC-CGACAGGTTCAGAGTTCTACAGTCCGA 44 + .|.|| ||||.|| | || |.|| ||| || +FC12044_91407 1 -GTCAT--GGCGGCC-CGCG-CGGG----GAG----------CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 -----------CCG-ACAGGTTCAGAGTTCTACAGTCCGACATG 32 + ||| .|.|| ||| || +FC12044_91407 1 GTCATGGCGGCCCGCGCGGG----GAG----------CG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Smal 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 13/34 (38.2%) +# Similarity: 13/34 (38.2%) +# Gaps: 17/34 (50.0%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGTCCGACGATC-------- 26 + | |||..| |.|.||| || | +FC12044_91407 1 G-TCATGG-----CGGCCCG-CG--CGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 12/34 (35.3%) +# Similarity: 12/34 (35.3%) +# Gaps: 21/34 (61.8%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATG-----CCGTCTTC------TGCTTGT 22 + || ||| ||| | .|| | +FC12044_91407 1 GTC--ATGGCGGCCCG----CGCGGGGAGC--G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Smal 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCAC-CGACAGGTTCAGAGTTCTACAGTCCGA 44 + .|.|| ||||.|| | || |.|| ||| || +FC12044_91407 1 -GTCAT--GGCGGCC-CGCG-CGGG----GAG----------CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 45.0 +# +# +#======================================= + +Illumina_Smal 1 CGACA-GGTTCAGAGTTCTACAGTCCGACGATC-------- 32 + |.|| || |.|.||| || | +FC12044_91407 1 -GTCATGG------------CGGCCCG-CG--CGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 30/44 (68.2%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Geno 1 -------GATC----GGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + |.|| .|||||| ||| +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAG-TCG------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Geno 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 37.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGA-GATCTACACTCTTTCCCTACACGA----- 44 + ||.|. |.| ||||. |.|| +FC12044_91407 1 ---------------ACAGTGGGT------TCTTA------AAGAAGAGT 23 + +Illumina_Geno 45 CGCTCTTCCGATCT 58 + || +FC12044_91407 24 CG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 11/48 (22.9%) +# Similarity: 11/48 (22.9%) +# Gaps: 37/48 (77.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 ----------C--AAGCAGAAGA--CGGCATACGAGCTCTTCCGATCT 34 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG--------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Geno 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 17/36 (47.2%) +# Similarity: 17/36 (47.2%) +# Gaps: 15/36 (41.7%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTC----AGCAGGAATGCCGAG 32 + .| ||.| ||||| || |.||.| || +FC12044_91407 1 --AC---AGTG-GGTTCTTAAAG-AAGAGT--CG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Pair 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 37.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGA-GATCTACACTCTTTCCCTACACGA----- 44 + ||.|. |.| ||||. |.|| +FC12044_91407 1 ---------------ACAGTGGGT------TCTTA------AAGAAGAGT 23 + +Illumina_Pair 45 CGCTCTTCCGATCT 58 + || +FC12044_91407 24 CG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 14/68 (20.6%) +# Similarity: 14/68 (20.6%) +# Gaps: 50/68 (73.5%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGG---CATACGAGA----TCGGTCTCGGCATTCCTGCT 43 + .|||. || |.|| .||| ||| +FC12044_91407 1 ---ACAGT----GGGTTCTTA-AAGAAGAGTCG----------------- 25 + +Illumina_Pair 44 GAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Pair 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 16/39 (41.0%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Pair 1 -CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGA-TCT 37 + |.|| || .|||.|...||| || ||. +FC12044_91407 1 ACAGT---GG-GTTCTTAAAGAA----------GAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 12/35 (34.3%) +# Similarity: 12/35 (34.3%) +# Gaps: 19/35 (54.3%) +# Score: 48.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCT----GAA----C- 26 + ||.||.|.. ||| ||| | +FC12044_91407 1 ---------ACAGTGGGT-TCTTAAAGAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 15/36 (41.7%) +# Similarity: 15/36 (41.7%) +# Gaps: 21/36 (58.3%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 ACA---GGTTC-------AGAGTTCTACAGTCCGAC 26 + ||| ||||| ||||| || +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGT---------CG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 7/36 (19.4%) +# Similarity: 7/36 (19.4%) +# Gaps: 26/36 (72.2%) +# Score: 24.5 +# +# +#======================================= + +Illumina_DpnI 1 ---------TCGTA------TGCCGTCTTCTGCTTG 21 + ||.|| .|.|| +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGTCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 15/54 (27.8%) +# Similarity: 15/54 (27.8%) +# Gaps: 39/54 (72.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACA---GGTTC-------AGAGTTCTACAGT 40 + ||| ||||| ||||| +FC12044_91407 1 -------------------ACAGTGGGTTCTTAAAGAAGAGT-------- 23 + +Illumina_DpnI 41 CCGA 44 + || +FC12044_91407 24 -CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 11/34 (32.4%) +# Score: 67.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGTCCGACGA-TC- 32 + |||| .|.|||||..|. ||.|| || +FC12044_91407 1 --ACAG----TGGGTTCTTAAA---GAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 48.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCT----GAA----C- 21 + ||.||.|.. ||| ||| | +FC12044_91407 1 ----ACAGTGGGT-TCTTAAAGAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 24/39 (61.5%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 ACA---GGTTC-------AGAGTTCTACAGTCCGACATG 29 + ||| ||||| ||||| || +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGT---------CG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 11/37 (29.7%) +# Similarity: 11/37 (29.7%) +# Gaps: 26/37 (70.3%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------C--AAGCAGAAGA--CGGCATACGANN 23 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 7/36 (19.4%) +# Similarity: 7/36 (19.4%) +# Gaps: 26/36 (72.2%) +# Score: 24.5 +# +# +#======================================= + +Illumina_NlaI 1 ---------TCGTA------TGCCGTCTTCTGCTTG 21 + ||.|| .|.|| +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGTCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 15/54 (27.8%) +# Similarity: 15/54 (27.8%) +# Gaps: 39/54 (72.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACA---GGTTC-------AGAGTTCTACAGT 40 + ||| ||||| ||||| +FC12044_91407 1 -------------------ACAGTGGGTTCTTAAAGAAGAGT-------- 23 + +Illumina_NlaI 41 CCGA 44 + || +FC12044_91407 24 -CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 15/42 (35.7%) +# Similarity: 15/42 (35.7%) +# Gaps: 27/42 (64.3%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACA---GGTTC-------AGAGTTCTACAGTCCGACATG 32 + ||| ||||| ||||| || +FC12044_91407 1 ---ACAGTGGGTTCTTAAAGAAGAGT---------CG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 15/30 (50.0%) +# Similarity: 15/30 (50.0%) +# Gaps: 9/30 (30.0%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGA--GTTCTACAGTCCGACGA-TC- 26 + .|||. |||||..|. ||.|| || +FC12044_91407 1 --ACAGTGGGTTCTTAAA---GAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 7/38 (18.4%) +# Similarity: 7/38 (18.4%) +# Gaps: 29/38 (76.3%) +# Score: 25.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCTTCTG------CTT--------GT-- 22 + .|.| ||| || +FC12044_91407 1 -------------ACAGTGGGTTCTTAAAGAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 15/54 (27.8%) +# Similarity: 15/54 (27.8%) +# Gaps: 39/54 (72.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACA---GGTTC-------AGAGTTCTACAGT 40 + ||| ||||| ||||| +FC12044_91407 1 -------------------ACAGTGGGTTCTTAAAGAAGAGT-------- 23 + +Illumina_Smal 41 CCGA 44 + || +FC12044_91407 24 -CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 11/34 (32.4%) +# Score: 67.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGTCCGACGA-TC- 32 + |||| .|.|||||..|. ||.|| || +FC12044_91407 1 --ACAG----TGGGTTCTTAAA---GAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 18/37 (48.6%) +# Similarity: 18/37 (48.6%) +# Gaps: 16/37 (43.2%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGC-CGTCTT--CTGCTTG- 33 + |.|| ||.| || ||.||| | |||| +FC12044_91407 1 -AACG--AGGG-------GCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 19/62 (30.6%) +# Similarity: 19/62 (30.6%) +# Gaps: 41/62 (66.1%) +# Score: 65.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATAC----GGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + .|| |||| ||| ||| ||| +FC12044_91407 1 -----AACGAGGGGCG-----CGA----------CTT---------GAC- 20 + +Illumina_Geno 47 CTCTTCCGATCT 58 + ||| |. +FC12044_91407 21 --CTT--GG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 18/37 (48.6%) +# Similarity: 18/37 (48.6%) +# Gaps: 15/37 (40.5%) +# Score: 64.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT--- 34 + |.| ||.| ||| .||| ||| ||.|| +FC12044_91407 1 --AAC-GAGG--GGC--GCGA---CTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 15/33 (45.5%) +# Similarity: 15/33 (45.5%) +# Gaps: 9/33 (27.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG- 32 + |.| ||.|.||| |..|..|| ||..| +FC12044_91407 1 -AAC-GAGGGGCG---CGACTTGA---CCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 19/62 (30.6%) +# Similarity: 19/62 (30.6%) +# Gaps: 41/62 (66.1%) +# Score: 65.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATAC----GGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + .|| |||| ||| ||| ||| +FC12044_91407 1 -----AACGAGGGGCG-----CGA----------CTT---------GAC- 20 + +Illumina_Pair 47 CTCTTCCGATCT 58 + ||| |. +FC12044_91407 21 --CTT--GG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 38/62 (61.3%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCAT-TCCTGCTGAACCG 49 + .||||| ||.|.||.|.| .||| ||. +FC12044_91407 1 ----------------AACGAG---GGGCGCGACTTGACCT--TGG---- 25 + +Illumina_Pair 50 CTCTTCCGATCT 61 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 13/47 (27.7%) +# Similarity: 13/47 (27.7%) +# Gaps: 32/47 (68.1%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAAC------CGC-TCTTCCGATCT--- 37 + ||| ||| .||| ||.|| +FC12044_91407 1 --------------------AACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 15/31 (48.4%) +# Similarity: 15/31 (48.4%) +# Gaps: 11/31 (35.5%) +# Score: 56.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGT----CG-GACTGTAGAACTCTGAAC 26 + |.||. || ||| |.||.|| ||. +FC12044_91407 1 -AACGAGGGGCGCGAC--TTGACCT-TGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 11/39 (28.2%) +# Similarity: 11/39 (28.2%) +# Gaps: 27/39 (69.2%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 -AC-AGGTTCAGAGTTCTACAGTC-CGAC---------- 26 + || ||| |.| |||| +FC12044_91407 1 AACGAGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 60.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGT-ATGC-CGTCTT--CTGCTTG- 21 + .||. ..|| ||.||| | |||| +FC12044_91407 1 AACGAGGGGCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 59.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC----GGCGACCACCGACAGGTTCAGAGTTCTACAGTCC---G 43 + .|| |||| |||| || |.|| | +FC12044_91407 1 -----AACGAGGGGCG-----CGAC---TT------------GACCTTGG 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 14/41 (34.1%) +# Similarity: 14/41 (34.1%) +# Gaps: 25/41 (61.0%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 --CGACAGGTTCAGAGTTCTACAGTC-CGAC--GATC---- 32 + || ||| |.| |||| ||.| +FC12044_91407 1 AACG--AGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 43.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCG--------GACTGTAGAACTCTGAAC 21 + .|| ||| |.||.|| ||. +FC12044_91407 1 AACGAGGGGCGCGAC--TTGACCT-TGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 13/39 (33.3%) +# Similarity: 13/39 (33.3%) +# Gaps: 24/39 (61.5%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 -AC-AGGTTCAGAGTTCTACAGTC-CGACATG------- 29 + || ||| |.| ||||.|| +FC12044_91407 1 AACGAGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 14/31 (45.2%) +# Score: 37.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG---ACGGCAT-AC----GANN 23 + |.| ||.| .||.|.| || | +FC12044_91407 1 --AAC-GAGGGGCGCGACTTGACCTTGG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 60.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGT-ATGC-CGTCTT--CTGCTTG- 21 + .||. ..|| ||.||| | |||| +FC12044_91407 1 AACGAGGGGCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 59.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC----GGCGACCACCGACAGGTTCAGAGTTCTACAGTCC---G 43 + .|| |||| |||| || |.|| | +FC12044_91407 1 -----AACGAGGGGCG-----CGAC---TT------------GACCTTGG 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 49.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGAC-AGGTTCAGAGTTCTACAGTC-CGACATG------- 32 + .|| ||| |.| ||||.|| +FC12044_91407 1 --AACGAGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 11/36 (30.6%) +# Similarity: 11/36 (30.6%) +# Gaps: 21/36 (58.3%) +# Score: 37.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTAC---AGTC-CGAC--GATC---- 26 + .|| .|.| |||| ||.| +FC12044_91407 1 -----------AACGAGGGGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 7/27 (25.9%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGT-ATGC-CGTCTT--CTGCTTGT 22 + .||. ..|| ||.||| | ||||. +FC12044_91407 1 AACGAGGGGCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC----GGCGACCACCGACAGGTTCAGAGTTCTACAGTCC---G 43 + .|| |||| |||| || |.|| | +FC12044_91407 1 -----AACGAGGGGCG-----CGAC---TT------------GACCTTGG 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 14/41 (34.1%) +# Similarity: 14/41 (34.1%) +# Gaps: 25/41 (61.0%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 --CGACAGGTTCAGAGTTCTACAGTC-CGAC--GATC---- 32 + || ||| |.| |||| ||.| +FC12044_91407 1 AACG--AGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 30/44 (68.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTAT---GCCGTCTTCTG-----CTTG--- 33 + |.| .|| | |.| |||| +FC12044_91407 1 ---------------TTTCCCACC--C--CAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC---GACGC 47 + |||||| ||.| ||.|| +FC12044_91407 1 -------------------------------TTTCCC-ACCCCAGGAAGC 18 + +Illumina_Geno 48 TCTTCCGATCT 58 + ||| |..| +FC12044_91407 19 -CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 27/43 (62.8%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Geno 1 ---------CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + | |||.| ||| ||| |..| +FC12044_91407 1 TTTCCCACCC---CAGGA-----------AGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 27/42 (64.3%) +# Score: 55.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTT-------CAGCAGGAATGCCGAG--- 32 + || | |||||| |||..| +FC12044_91407 1 --------------TTTCCCACCC--CAGGAA-GCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC---GACGC 47 + |||||| ||.| ||.|| +FC12044_91407 1 -------------------------------TTTCCC-ACCCCAGGAAGC 18 + +Illumina_Pair 48 TCTTCCGATCT 58 + ||| |..| +FC12044_91407 19 -CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 15/68 (22.1%) +# Similarity: 15/68 (22.1%) +# Gaps: 50/68 (73.5%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACC-- 48 + .|||| | ||| +FC12044_91407 1 ----------------------------------TTTCC--C---ACCCC 11 + +Illumina_Pair 49 -----GCTCTTCCGATCT 61 + || ||| |..| +FC12044_91407 12 AGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 15/44 (34.1%) +# Similarity: 15/44 (34.1%) +# Gaps: 26/44 (59.1%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACC-------GCTCTTCCGATCT 37 + .|||| | ||| || ||| |..| +FC12044_91407 1 ----------TTTCC--C---ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 38.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGT--------CGGACTGTAGAA--CTCTGAAC 26 + | | |.| ||| || ||.|| +FC12044_91407 1 -----TTTCCCACCC---CAG--GAAGCCT-TGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTAC-------AGTCC---GAC 26 + .||| |.|| || || ||| +FC12044_91407 1 ----TTTC------CCACCCCAGGAAG-CCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTAT---GCCGTCTTCTG-----CTTG--- 21 + |.| .|| | |.| |||| +FC12044_91407 1 ---TTTCCCACC--C--CAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTT-CAGAGTTCT---ACAGTCCGA 44 + .|.| ||||| .||||.. | || || +FC12044_91407 1 ----TTTC-----CCACC-CCAGGAAGC------CTTGGAC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 31/44 (70.5%) +# Score: 44.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------CGACAGGTTCAGAGTTCTACAGTCC---GACGATC 32 + | ||||. || || ||| +FC12044_91407 1 TTTCCCACCC--CAGGA--AG-----------CCTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 20/33 (60.6%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 --TC--------GGA--CTGTAGAACTCTGAAC 21 + || ||| | || ||.|| +FC12044_91407 1 TTTCCCACCCCAGGAAGC-------CT-TGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 13/38 (34.2%) +# Similarity: 13/38 (34.2%) +# Gaps: 22/38 (57.9%) +# Score: 44.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCC---GA---CATG--- 29 + .||| |.|| || || |.|| +FC12044_91407 1 ----TTTC------CCAC---CCCAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 20/34 (58.8%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 ---------CAAGCAGAAGACGGCAT--ACGANN 23 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTAT---GCCGTCTTCTG-----CTTG--- 21 + |.| .|| | |.| |||| +FC12044_91407 1 ---TTTCCCACC--C--CAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTT-CAGAGTTCT---ACAGTCCGA 44 + .|.| ||||| .||||.. | || || +FC12044_91407 1 ----TTTC-----CCACC-CCAGGAAGC------CTTGGAC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 29/43 (67.4%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 --------CCGACAGGTTCAGAGTTCTACAGTCC---GACATG 32 + || ||||. || || ||| +FC12044_91407 1 TTTCCCACCC--CAGGA--AG-----------CCTTGGAC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTAC-------AGTCC---GACGATC 26 + .||| |.|| || || ||| +FC12044_91407 1 TTTC------CCACCCCAGGAAG-CCTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 23/35 (65.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_Smal 1 --TC--------GTATGCC---GTCTTCTGCTTGT 22 + || |.|.||| |.| +FC12044_91407 1 TTTCCCACCCCAGGAAGCCTTGGAC---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTT-CAGAGTTCT---ACAGTCCGA 44 + .|.| ||||| .||||.. | || || +FC12044_91407 1 ----TTTC-----CCACC-CCAGGAAGC------CTTGGAC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 31/44 (70.5%) +# Score: 44.0 +# +# +#======================================= + +Illumina_Smal 1 ---------CGACAGGTTCAGAGTTCTACAGTCC---GACGATC 32 + | ||||. || || ||| +FC12044_91407 1 TTTCCCACCC--CAGGA--AG-----------CCTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 17/37 (45.9%) +# Similarity: 17/37 (45.9%) +# Gaps: 16/37 (43.2%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAG-CT-CGTATGCC--GTCTTCTGCTTG 33 + || || || || |||| |.|..||.| +FC12044_91407 1 --TC-----AGCCTCCG--TGCCCAGCCCACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Geno 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 58 +# Identity: 18/58 (31.0%) +# Similarity: 18/58 (31.0%) +# Gaps: 33/58 (56.9%) +# Score: 98.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + .|.| ||.|||.| |||..|.| || +FC12044_91407 1 ------TCAG---CCTCCGTG-------------CCCAGCCC-AC----- 22 + +Illumina_Geno 51 TCCGATCT 58 + ||| +FC12044_91407 23 TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 13/36 (36.1%) +# Score: 68.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-- 34 + .|.||.|.||.||.|..||.| || +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCA-CTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Geno 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 12/43 (27.9%) +# Similarity: 12/43 (27.9%) +# Gaps: 29/43 (67.4%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAG---CAGGAATGCCGAG-------- 32 + |||| |.| ||||.|| +FC12044_91407 1 ---------------TCAGCCTCCG---TGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 58 +# Identity: 18/58 (31.0%) +# Similarity: 18/58 (31.0%) +# Gaps: 33/58 (56.9%) +# Score: 98.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + .|.| ||.|||.| |||..|.| || +FC12044_91407 1 ------TCAG---CCTCCGTG-------------CCCAGCCC-AC----- 22 + +Illumina_Pair 51 TCCGATCT 58 + ||| +FC12044_91407 23 TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 63 +# Identity: 19/63 (30.2%) +# Similarity: 19/63 (30.2%) +# Gaps: 40/63 (63.5%) +# Score: 99.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCT-CG-GCATTCCTGCTGAACC 48 + ||.|.|| || || ||.|| || +FC12044_91407 1 -----------------------TCAGCCTCCGTGC---CCAGC----CC 20 + +Illumina_Pair 49 GCTCTTCCGATCT 61 + .||| | +FC12044_91407 21 ACTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 18/40 (45.0%) +# Similarity: 18/40 (45.0%) +# Gaps: 18/40 (45.0%) +# Score: 92.0 +# +# +#======================================= + +Illumina_Pair 1 -CGGTCT-CG-GCATTCCTGCTGAACCGCTCTTCCGATCT 37 + |.|.|| || || ||.|| ||.||| | +FC12044_91407 1 TCAGCCTCCGTGC---CCAGC----CCACTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 50.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACT--GT----AG--AACTCTGAAC 26 + ||.|.|| || || .|||| | +FC12044_91407 1 -----TCAGCCTCCGTGCCCAGCCCACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAG-----GTTCAGAGTTCTACAGTCCGAC--- 26 + .||| ||.| | |||.|| || +FC12044_91407 1 TCAGCCTCCGTGC------C--CAGCCC-ACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 43.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTCTTCTGCTTG------------- 21 + || .||| || |.|| +FC12044_91407 1 TC---AGCC-TC-----CGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 17/47 (36.2%) +# Similarity: 17/47 (36.2%) +# Gaps: 25/47 (53.2%) +# Score: 78.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGA---CAGGTTCAGAGTTCTACAGTCCGA 44 + .|.| ||.|||. ||| | |.|| ||| +FC12044_91407 1 ------TCAG---CCTCCGTGCCCAG---C------CCAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 16/38 (42.1%) +# Similarity: 16/38 (42.1%) +# Gaps: 19/38 (50.0%) +# Score: 59.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAG-----GTTCAGAGTTCTACAGTCCGACGATC- 32 + .||| ||.| | |||.|| || || +FC12044_91407 1 --TCAGCCTCCGTGC------C--CAGCCC-AC--TCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 12/29 (41.4%) +# Score: 50.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACT--GT----AG--AACTCTGAAC 21 + ||.|.|| || || .|||| | +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCCACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 12/42 (28.6%) +# Similarity: 12/42 (28.6%) +# Gaps: 30/42 (71.4%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCCGACATG------------- 29 + |||| | | |||| || +FC12044_91407 1 ------TCAG----C--C--TCCG---TGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 9/37 (24.3%) +# Similarity: 9/37 (24.3%) +# Gaps: 26/37 (70.3%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 -------------CAAGCAGAAGACGGCA-TACGANN 23 + |.||| | || |.| +FC12044_91407 1 TCAGCCTCCGTGCCCAGC------C--CACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 43.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTCTTCTGCTTG------------- 21 + || .||| || |.|| +FC12044_91407 1 TC---AGCC-TC-----CGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 17/47 (36.2%) +# Similarity: 17/47 (36.2%) +# Gaps: 25/47 (53.2%) +# Score: 78.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGA---CAGGTTCAGAGTTCTACAGTCCGA 44 + .|.| ||.|||. ||| | |.|| ||| +FC12044_91407 1 ------TCAG---CCTCCGTGCCCAG---C------CCAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 27/42 (64.3%) +# Score: 57.0 +# +# +#======================================= + +Illumina_NlaI 1 -------CCGA---CAGGTTCAGAGTTCTACAGTCCGACATG 32 + |||. ||| | |.|| ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAG---C------CCAC--TCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 16/31 (51.6%) +# Similarity: 16/31 (51.6%) +# Gaps: 11/31 (35.5%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAG----AGTTCTACAGTCCGACGATC- 26 + |||| .||.| .|||.|| || || +FC12044_91407 1 --TCAGCCTCCGTGC-CCAGCCC-AC--TCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 15/31 (48.4%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 ------T-CGTATGCC--GTCTTCTGCTTGT 22 + | || |||| |.|..||.| +FC12044_91407 1 TCAGCCTCCG--TGCCCAGCCCACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 17/47 (36.2%) +# Similarity: 17/47 (36.2%) +# Gaps: 25/47 (53.2%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGA---CAGGTTCAGAGTTCTACAGTCCGA 44 + .|.| ||.|||. ||| | |.|| ||| +FC12044_91407 1 ------TCAG---CCTCCGTGCCCAG---C------CCAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 16/38 (42.1%) +# Similarity: 16/38 (42.1%) +# Gaps: 19/38 (50.0%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAG-----GTTCAGAGTTCTACAGTCCGACGATC- 32 + .||| ||.| | |||.|| || || +FC12044_91407 1 --TCAGCCTCCGTGC------C--CAGCCC-AC--TCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 12/43 (27.9%) +# Similarity: 12/43 (27.9%) +# Gaps: 28/43 (65.1%) +# Score: 44.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCT----C------GTATGCCGTCTTCTGCTTG 33 + .||||.|| ||| | ||. +FC12044_91407 1 -CTCGGGAG-GCTGAGGCAGGGGGGTT---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 71 +# Identity: 10/71 (14.1%) +# Similarity: 10/71 (14.1%) +# Gaps: 59/71 (83.1%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC--GACGCT 48 + |.| ||.||| +FC12044_91407 1 ---------------------------------------CTCGGGAGGCT 11 + +Illumina_Geno 49 ----C-------TTCCGATCT 58 + | || +FC12044_91407 12 GAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 15/37 (40.5%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGAC---GGCATACGAGCTCTTCCGATCT 34 + |. |.|.||.| ||||...|.| || +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGG---TT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 13/35 (37.1%) +# Score: 57.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG--- 32 + .||||.|| ||.|. .||||| |.| +FC12044_91407 1 -CTCGGGAG-GCTGA--GGCAGG------GGGGTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 71 +# Identity: 10/71 (14.1%) +# Similarity: 10/71 (14.1%) +# Gaps: 59/71 (83.1%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC--GACGCT 48 + |.| ||.||| +FC12044_91407 1 ---------------------------------------CTCGGGAGGCT 11 + +Illumina_Pair 49 ----C-------TTCCGATCT 58 + | || +FC12044_91407 12 GAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 14/69 (20.3%) +# Similarity: 14/69 (20.3%) +# Gaps: 52/69 (75.4%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + |||||.| .|||||. || +FC12044_91407 1 ----------------------------CTCGGGA----GGCTGAG--GC 16 + +Illumina_Pair 51 --------TCTTCCGATCT 61 + | +FC12044_91407 17 AGGGGGGTT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 14/45 (31.1%) +# Similarity: 14/45 (31.1%) +# Gaps: 28/45 (62.2%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGC--------TCTTCCGATCT 37 + |||||.| .|||||. || | +FC12044_91407 1 ----CTCGGGA----GGCTGAG--GCAGGGGGGTT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 21/36 (58.3%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGA-AC--------- 26 + | |||| |..| |||| .| +FC12044_91407 1 ---C-TCGG---GAGG----CTGAGGCAGGGGGGTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 8/40 (20.0%) +# Similarity: 8/40 (20.0%) +# Gaps: 29/40 (72.5%) +# Score: 37.0 +# +# +#======================================= + +Illumina_DpnI 1 --------------ACAGGTTCAGAGTTCTACAGTCCGAC 26 + .|||| .|.||| +FC12044_91407 1 CTCGGGAGGCTGAGGCAGG---GGGGTT------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 27.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATGCCGTCTTCT---GC-------TTG 21 + |||...| | || || || +FC12044_91407 1 CTCGGGAG--G----CTGAGGCAGGGGGGTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + | ||| || |.|||| .|.||| +FC12044_91407 1 -------CTCGGGAGGCTGA-----GGCAGG---GGGGTT---------- 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 --CGACAGGTTCAGAGTTCTACAG------TCCGACGATC 32 + ||..||| |.||| .||| | +FC12044_91407 1 CTCGGGAGG--CTGAG----GCAGGGGGGTT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 -TCGGACTGTAGAACTCTGA-AC--------- 21 + |||| |..| |||| .| +FC12044_91407 1 CTCGG---GAGG----CTGAGGCAGGGGGGTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 8/43 (18.6%) +# Similarity: 8/43 (18.6%) +# Gaps: 32/43 (74.4%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 --------------ACAGGTTCAGAGTTCTACAGTCCGACATG 29 + .|||| .|.||| +FC12044_91407 1 CTCGGGAGGCTGAGGCAGG---GGGGTT--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 18/33 (54.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC---GGCA-------TACGANN 23 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 27.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATGCCGTCTTCT---GC-------TTG 21 + |||...| | || || || +FC12044_91407 1 CTCGGGAG--G----CTGAGGCAGGGGGGTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + | ||| || |.|||| .|.||| +FC12044_91407 1 -------CTCGGGAGGCTGA-----GGCAGG---GGGGTT---------- 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 29/43 (67.4%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 ---------CCGA--CAGGTTCAGAGTTCTACAGTCCGACATG 32 + |.|| |||| .|.||| +FC12044_91407 1 CTCGGGAGGCTGAGGCAGG---GGGGTT--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 9/40 (22.5%) +# Similarity: 9/40 (22.5%) +# Gaps: 29/40 (72.5%) +# Score: 25.5 +# +# +#======================================= + +Illumina_Smal 1 --------GTTCAGAGTTCTACAG------TCCGACGATC 26 + | |.||| .||| | +FC12044_91407 1 CTCGGGAGG--CTGAG----GCAGGGGGGTT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 19/33 (57.6%) +# Score: 27.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATGCCGTCTTCT---GC-------TTGT 22 + |||...| | || || || +FC12044_91407 1 CTCGGGAG--G----CTGAGGCAGGGGGGTT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + | ||| || |.|||| .|.||| +FC12044_91407 1 -------CTCGGGAGGCTGA-----GGCAGG---GGGGTT---------- 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 --CGACAGGTTCAGAGTTCTACAG------TCCGACGATC 32 + ||..||| |.||| .||| | +FC12044_91407 1 CTCGGGAGG--CTGAG----GCAGGGGGGTT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 16/40 (40.0%) +# Similarity: 16/40 (40.0%) +# Gaps: 22/40 (55.0%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ---GATC-GGAA--G-AGCTCGTATGCCGTCTTCTGCTTG 33 + .||| .||| | ||||| || || +FC12044_91407 1 CCAAATCTTGAATTGTAGCTC-----CC--CT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 65 +# Identity: 15/65 (23.1%) +# Similarity: 15/65 (23.1%) +# Gaps: 47/65 (72.3%) +# Score: 80.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACA-------CTCTTTCCCTACACG 43 + || |.||||..| ||| |||| +FC12044_91407 1 ----------------CC-AAATCTTGAATTGTAGCTC---CCCT----- 25 + +Illumina_Geno 44 ACGCTCTTCCGATCT 58 + +FC12044_91407 26 --------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 17/39 (43.6%) +# Similarity: 17/39 (43.6%) +# Gaps: 19/39 (48.7%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 -CAAG-C-AGAA--GACGGCATACGAGCTCTTCCGATCT 34 + |||. | .||| |. ||||| || || +FC12044_91407 1 CCAAATCTTGAATTGT---------AGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 21/39 (53.8%) +# Score: 37.0 +# +# +#======================================= + +Illumina_Pair 1 ---GATC-GGAA--G-AGCGGTTCAGCAGGAATGCCGAG 32 + .||| .||| | ||| || | ||. +FC12044_91407 1 CCAAATCTTGAATTGTAGC---TC--C-------CCT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 65 +# Identity: 15/65 (23.1%) +# Similarity: 15/65 (23.1%) +# Gaps: 47/65 (72.3%) +# Score: 80.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACA-------CTCTTTCCCTACACG 43 + || |.||||..| ||| |||| +FC12044_91407 1 ----------------CC-AAATCTTGAATTGTAGCTC---CCCT----- 25 + +Illumina_Pair 44 ACGCTCTTCCGATCT 58 + +FC12044_91407 26 --------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 21/61 (34.4%) +# Similarity: 21/61 (34.4%) +# Gaps: 36/61 (59.0%) +# Score: 74.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + | ||.| |||.| |.| ||.| || +FC12044_91407 1 -----------C--CAAA---------TCTTG--AAT-----TGTA--GC 19 + +Illumina_Pair 51 TCTTCCGATCT 61 + || || || +FC12044_91407 20 TC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 17/41 (41.5%) +# Similarity: 17/41 (41.5%) +# Gaps: 20/41 (48.8%) +# Score: 66.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGC--ATTCCTGC--TGAACCGCTCTTCCGATCT 37 + | |.||.||. ||.| |||| || || +FC12044_91407 1 ---------CCAAATCTTGAATTGTA--GCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 15/32 (46.9%) +# Similarity: 15/32 (46.9%) +# Gaps: 13/32 (40.6%) +# Score: 61.0 +# +# +#======================================= + +Illumina_DpnI 1 ---GATCGTCGGACTGTAGAACTCTGAAC--- 26 + .|||.| |.|.||||| ||| | +FC12044_91407 1 CCAAATCTT-GAATTGTAG--CTC----CCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 9/30 (30.0%) +# Score: 49.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTC-AGAGTTCTA-CAGTCCGAC-- 26 + |....|| .||.||.|| | ||| | +FC12044_91407 1 -CCAAATCTTGAATTGTAGC--TCC--CCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 8/37 (21.6%) +# Similarity: 8/37 (21.6%) +# Gaps: 28/37 (75.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCC--GTCTT--------------CTGCTTG 21 + || .|||| || +FC12044_91407 1 -------CCAAATCTTGAATTGTAGCTCCCCT----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 14/49 (28.6%) +# Similarity: 14/49 (28.6%) +# Gaps: 29/49 (59.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTT----CTACAGTCCGA 44 + ||.| .|| .||.|| ||.| ||. +FC12044_91407 1 ----------------CCAA----ATCTTGAATTGTAGCTCC---CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 17/35 (48.6%) +# Similarity: 17/35 (48.6%) +# Gaps: 13/35 (37.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTC-AGAGTTCTA-CAGTCCGACGATC- 32 + | || ..|| .||.||.|| | ||| | | +FC12044_91407 1 C--CA-AATCTTGAATTGTAGC--TCC--C---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 13/31 (41.9%) +# Similarity: 13/31 (41.9%) +# Gaps: 16/31 (51.6%) +# Score: 55.5 +# +# +#======================================= + +Illumina_NlaI 1 -----TC--GGACTGTAGAACTCTGAAC--- 21 + || |.|.||||| ||| | +FC12044_91407 1 CCAAATCTTGAATTGTAG--CTC----CCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 15/31 (48.4%) +# Similarity: 15/31 (48.4%) +# Gaps: 8/31 (25.8%) +# Score: 50.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTC-AGAGTTCTA-CAGTCCGACATG 29 + |....|| .||.||.|| | ||| |.| +FC12044_91407 1 -CCAAATCTTGAATTGTAGC--TCC--CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 18/33 (54.5%) +# Score: 20.0 +# +# +#======================================= + +Illumina_NlaI 1 -CAAG-C-AGAA--GA-----CGGCATACGANN 23 + |||. | .||| |. | |.| +FC12044_91407 1 CCAAATCTTGAATTGTAGCTCC--CCT------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 8/37 (21.6%) +# Similarity: 8/37 (21.6%) +# Gaps: 28/37 (75.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCC--GTCTT--------------CTGCTTG 21 + || .|||| || +FC12044_91407 1 -------CCAAATCTTGAATTGTAGCTCCCCT----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 14/49 (28.6%) +# Similarity: 14/49 (28.6%) +# Gaps: 29/49 (59.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTT----CTACAGTCCGA 44 + ||.| .|| .||.|| ||.| ||. +FC12044_91407 1 ----------------CCAA----ATCTTGAATTGTAGCTCC---CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 11/34 (32.4%) +# Score: 62.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTC-AGAGTTCTA-CAGTCCGACATG 32 + ||.| .|| .||.||.|| | ||| |.| +FC12044_91407 1 CCAA----ATCTTGAATTGTAGC--TCC--CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 13/32 (40.6%) +# Score: 46.0 +# +# +#======================================= + +Illumina_Smal 1 ---GTTC-AGAGTTCTA-CAGTCCGACGATC- 26 + ..|| .||.||.|| | ||| | | +FC12044_91407 1 CCAAATCTTGAATTGTAGC--TCC--C---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 21/34 (61.8%) +# Score: 45.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCC--GTCTTCTG-CTTGT--------- 22 + || .||| || .|||| +FC12044_91407 1 -------CCAAATCT--TGAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 14/49 (28.6%) +# Similarity: 14/49 (28.6%) +# Gaps: 29/49 (59.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTT----CTACAGTCCGA 44 + ||.| .|| .||.|| ||.| ||. +FC12044_91407 1 ----------------CCAA----ATCTTGAATTGTAGCTCC---CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 17/35 (48.6%) +# Similarity: 17/35 (48.6%) +# Gaps: 13/35 (37.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTC-AGAGTTCTA-CAGTCCGACGATC- 32 + | || ..|| .||.||.|| | ||| | | +FC12044_91407 1 C--CA-AATCTTGAATTGTAGC--TCC--C---CT 25 + + +#--------------------------------------- +#---------------------------------------
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/emboss_needleall_out.score Mon Jan 20 16:22:05 2025 +0000 @@ -0,0 +1,777 @@ +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_406_24 38 (21.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_406_24 39 (24.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_406_24 60 (31.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_406_24 44 (25.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_406_24 39 (24.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_406_24 49 (16.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_406_24 39 (24.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_406_24 60 (31.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_406_24 67 (21.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_406_24 39 (24.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_406_24 43 (21.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_406_24 38 (14.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_406_24 40 (24.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_406_24 29 (12.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_406_24 30 (12.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_406_24 29 (12.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_406_24 45 (41.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_406_24 42 (23.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_406_24 34 (12.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_406_24 40 (27.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_406_24 31 (12.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_406_24 30 (12.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_406_24 29 (12.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_406_24 45 (41.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_406_24 40 (27.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_406_24 29 (12.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_406_24 36 (23.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_406_24 31 (13.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_406_24 29 (12.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_406_24 45 (41.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_406_24 42 (23.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_720_610 44 (17.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_720_610 40 (31.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_720_610 61 (31.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_720_610 47 (20.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_720_610 40 (31.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_720_610 40 (31.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_720_610 61 (31.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_720_610 64 (33.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_720_610 40 (31.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_720_610 40 (33.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_720_610 43 (20.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_720_610 40 (9.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_720_610 36 (11.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_720_610 39 (15.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_720_610 36 (11.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_720_610 59 (10.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_720_610 44 (15.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_720_610 38 (20.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_720_610 42 (9.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_720_610 36 (7.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_720_610 39 (15.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_720_610 36 (11.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_720_610 59 (10.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_720_610 45 (9.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_720_610 36 (11.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_720_610 38 (15.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_720_610 42 (15.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_720_610 36 (11.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_720_610 59 (10.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_720_610 44 (15.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_345_133 47 (8.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_345_133 53 (16.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_345_133 69 (20.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_345_133 45 (21.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_345_133 53 (16.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_345_133 54 (6.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_345_133 53 (16.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_345_133 69 (20.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_345_133 72 (21.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_345_133 53 (16.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_345_133 57 (16.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_345_133 33 (27.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_345_133 40 (19.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_345_133 32 (21.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_345_133 41 (7.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_345_133 32 (21.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_345_133 56 (16.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_345_133 37 (16.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_345_133 28 (27.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_345_133 34 (24.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_345_133 34 (21.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_345_133 41 (7.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_345_133 32 (21.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_345_133 56 (16.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_345_133 37 (24.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_345_133 32 (21.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_345_133 44 (17.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_345_133 41 (12.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_345_133 32 (21.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_345_133 56 (16.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_345_133 37 (16.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_106_131 43 (32.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_106_131 36 (33.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_106_131 61 (33.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_106_131 42 (21.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_106_131 36 (33.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_106_131 48 (21.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_106_131 36 (33.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_106_131 61 (33.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_106_131 64 (31.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_106_131 36 (33.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_106_131 43 (33.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_106_131 35 (40.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_106_131 31 (26.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_106_131 32 (11.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_106_131 31 (32.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_106_131 32 (11.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_106_131 52 (24.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_106_131 37 (24.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_106_131 32 (32.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_106_131 36 (26.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_106_131 32 (7.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_106_131 31 (32.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_106_131 32 (11.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_106_131 52 (24.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_106_131 36 (29.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_106_131 32 (11.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_106_131 34 (24.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_106_131 32 (32.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_106_131 32 (11.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_106_131 52 (24.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_106_131 37 (24.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_916_471 37 (25.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_916_471 52 (12.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_916_471 73 (18.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_916_471 43 (20.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_916_471 52 (12.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_916_471 35 (32.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_916_471 52 (12.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_916_471 73 (18.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_916_471 70 (20.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_916_471 52 (12.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_916_471 49 (17.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_916_471 31 (35.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_916_471 34 (18.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_916_471 30 (20.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_916_471 40 (21.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_916_471 30 (20.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_916_471 46 (24.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_916_471 38 (19.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_916_471 31 (28.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_916_471 37 (18.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_916_471 32 (20.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_916_471 40 (21.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_916_471 30 (20.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_916_471 46 (24.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_916_471 37 (15.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_916_471 30 (20.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_916_471 37 (13.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_916_471 40 (17.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_916_471 30 (20.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_916_471 46 (24.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_916_471 38 (19.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_57_85 39 (17.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_57_85 45 (13.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_57_85 66 (15.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_57_85 45 (29.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_57_85 45 (13.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_57_85 35 (41.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_57_85 45 (13.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_57_85 66 (15.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_57_85 72 (29.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_57_85 45 (13.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_57_85 50 (15.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_57_85 40 (20.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_57_85 38 (11.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_57_85 32 (29.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_57_85 29 (7.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_57_85 32 (29.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_57_85 51 (18.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_57_85 45 (12.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_57_85 28 (18.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_57_85 38 (16.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_57_85 34 (29.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_57_85 29 (7.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_57_85 32 (29.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_57_85 51 (18.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_57_85 38 (22.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_57_85 32 (29.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_57_85 38 (12.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_57_85 30 (7.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_57_85 32 (29.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_57_85 51 (18.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_57_85 45 (12.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_10_437 50 (31.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_10_437 45 (18.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_10_437 70 (18.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_10_437 49 (23.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_10_437 45 (18.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_10_437 36 (29.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_10_437 45 (18.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_10_437 70 (18.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_10_437 76 (23.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_10_437 45 (18.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_10_437 48 (20.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_10_437 37 (13.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_10_437 44 (15.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_10_437 36 (23.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_10_437 38 (31.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_10_437 36 (23.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_10_437 51 (17.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_10_437 48 (16.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_10_437 35 (14.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_10_437 47 (15.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_10_437 38 (23.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_10_437 38 (31.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_10_437 36 (23.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_10_437 51 (17.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_10_437 47 (19.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_10_437 36 (23.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_10_437 43 (10.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_10_437 38 (27.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_10_437 36 (23.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_10_437 51 (17.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_10_437 48 (16.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_154_436 40 (22.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_154_436 38 (33.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_154_436 63 (33.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_154_436 41 (26.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_154_436 38 (33.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_154_436 37 (22.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_154_436 38 (33.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_154_436 63 (33.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_154_436 65 (36.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_154_436 38 (33.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_154_436 43 (18.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_154_436 31 (42.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_154_436 29 (25.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_154_436 34 (29.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_154_436 33 (16.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_154_436 34 (29.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_154_436 58 (27.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_154_436 34 (21.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_154_436 31 (35.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_154_436 31 (21.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_154_436 34 (25.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_154_436 33 (16.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_154_436 34 (29.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_154_436 58 (27.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_154_436 34 (21.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_154_436 34 (29.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_154_436 32 (19.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_154_436 34 (16.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_154_436 34 (29.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_154_436 58 (27.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_154_436 34 (21.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_336_64 44 (29.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_336_64 38 (22.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_336_64 61 (19.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_336_64 39 (34.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_336_64 38 (22.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_336_64 35 (35.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_336_64 38 (22.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_336_64 61 (19.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_336_64 66 (26.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_336_64 38 (22.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_336_64 46 (18.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_336_64 40 (16.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_336_64 33 (29.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_336_64 28 (23.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_336_64 36 (18.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_336_64 28 (23.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_336_64 53 (30.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_336_64 39 (29.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_336_64 35 (10.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_336_64 36 (29.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_336_64 30 (23.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_336_64 36 (18.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_336_64 28 (23.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_336_64 53 (30.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_336_64 39 (29.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_336_64 28 (23.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_336_64 35 (20.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_336_64 37 (18.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_336_64 28 (23.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_336_64 53 (30.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_336_64 39 (29.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_620_233 50 (22.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_620_233 40 (20.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_620_233 61 (28.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_620_233 50 (16.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_620_233 40 (20.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_620_233 44 (15.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_620_233 40 (20.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_620_233 61 (28.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_620_233 67 (33.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_620_233 40 (20.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_620_233 43 (33.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_620_233 34 (27.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_620_233 38 (26.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_620_233 34 (6.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_620_233 38 (22.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_620_233 34 (6.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_620_233 57 (21.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_620_233 40 (27.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_620_233 34 (20.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_620_233 39 (26.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_620_233 36 (6.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_620_233 38 (22.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_620_233 34 (6.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_620_233 57 (21.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_620_233 42 (26.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_620_233 34 (6.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_620_233 34 (27.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_620_233 38 (27.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_620_233 34 (6.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_620_233 57 (21.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_620_233 40 (27.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_902_349 43 (25.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_902_349 42 (11.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_902_349 68 (32.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_902_349 40 (16.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_902_349 42 (11.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_902_349 46 (28.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_902_349 42 (11.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_902_349 68 (32.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_902_349 64 (22.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_902_349 42 (11.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_902_349 53 (18.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_902_349 34 (25.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_902_349 32 (16.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_902_349 35 (16.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_902_349 34 (13.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_902_349 35 (16.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_902_349 54 (32.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_902_349 39 (20.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_902_349 41 (25.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_902_349 35 (16.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_902_349 37 (16.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_902_349 34 (13.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_902_349 35 (16.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_902_349 54 (32.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_902_349 38 (16.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_902_349 35 (16.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_902_349 34 (16.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_902_349 35 (13.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_902_349 35 (16.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_902_349 54 (32.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_902_349 39 (20.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_40_618 44 (10.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_40_618 40 (30.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_40_618 65 (30.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_40_618 57 (10.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_40_618 40 (30.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_40_618 36 (12.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_40_618 40 (30.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_40_618 65 (30.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_40_618 72 (16.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_40_618 40 (30.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_40_618 48 (16.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_40_618 45 (12.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_40_618 42 (17.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_40_618 38 (5.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_40_618 32 (10.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_40_618 38 (5.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_40_618 61 (13.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_40_618 50 (13.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_40_618 40 (12.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_40_618 43 (18.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_40_618 44 (2.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_40_618 32 (10.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_40_618 38 (5.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_40_618 61 (13.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_40_618 46 (18.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_40_618 38 (5.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_40_618 44 (13.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_40_618 43 (11.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_40_618 38 (5.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_40_618 61 (13.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_40_618 50 (13.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_83_511 35 (31.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_83_511 42 (28.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_83_511 64 (42.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_83_511 42 (22.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_83_511 42 (28.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_83_511 48 (12.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_83_511 42 (28.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_83_511 64 (42.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_83_511 69 (25.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_83_511 42 (28.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_83_511 45 (25.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_83_511 37 (30.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_83_511 34 (28.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_83_511 36 (12.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_83_511 30 (23.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_83_511 36 (12.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_83_511 53 (23.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_83_511 39 (22.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_83_511 32 (30.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_83_511 34 (25.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_83_511 38 (12.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_83_511 30 (23.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_83_511 36 (12.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_83_511 53 (23.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_83_511 37 (25.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_83_511 36 (12.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_83_511 33 (22.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_83_511 31 (23.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_83_511 36 (12.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_83_511 53 (23.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_83_511 39 (22.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_76_246 47 (21.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_76_246 50 (18.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_76_246 75 (18.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_76_246 51 (18.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_76_246 50 (18.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_76_246 36 (25.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_76_246 50 (18.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_76_246 75 (18.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_76_246 65 (29.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_76_246 50 (18.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_76_246 48 (21.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_76_246 39 (20.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_76_246 36 (23.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_76_246 38 (13.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_76_246 38 (17.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_76_246 38 (13.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_76_246 54 (23.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_76_246 42 (23.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_76_246 28 (14.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_76_246 39 (23.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_76_246 40 (13.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_76_246 38 (17.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_76_246 38 (13.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_76_246 54 (23.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_76_246 42 (23.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_76_246 38 (13.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_76_246 36 (23.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_76_246 30 (14.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_76_246 38 (13.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_76_246 54 (23.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_76_246 42 (23.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_303_427 35 (29.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_303_427 47 (31.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_303_427 59 (35.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_303_427 45 (27.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_303_427 47 (31.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_303_427 46 (14.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_303_427 47 (31.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_303_427 59 (35.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_303_427 65 (25.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_303_427 47 (31.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_303_427 41 (25.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_303_427 29 (17.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_303_427 33 (40.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_303_427 40 (12.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_303_427 30 (22.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_303_427 40 (12.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_303_427 51 (40.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_303_427 38 (44.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_303_427 28 (16.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_303_427 36 (40.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_303_427 40 (8.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_303_427 30 (22.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_303_427 40 (12.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_303_427 51 (40.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_303_427 39 (40.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_303_427 40 (12.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_303_427 33 (40.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_303_427 30 (27.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_303_427 40 (12.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_303_427 51 (40.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_303_427 38 (44.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_31_299 39 (26.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_31_299 36 (34.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_31_299 60 (37.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_31_299 44 (27.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_31_299 36 (34.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_31_299 39 (16.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_31_299 36 (34.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_31_299 60 (37.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_31_299 66 (32.5) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_31_299 36 (34.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_31_299 42 (32.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_31_299 44 (17.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_31_299 35 (30.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_31_299 31 (27.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_31_299 38 (22.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_31_299 31 (27.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_31_299 54 (26.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_31_299 39 (29.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_31_299 39 (17.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_31_299 35 (28.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_31_299 33 (27.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_31_299 38 (22.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_31_299 31 (27.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_31_299 54 (26.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_31_299 38 (28.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_31_299 31 (27.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_31_299 33 (29.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_31_299 41 (19.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_31_299 31 (27.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_31_299 54 (26.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_31_299 39 (29.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_553_135 45 (15.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_553_135 49 (9.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_553_135 61 (25.5) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_553_135 37 (20.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_553_135 49 (9.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_553_135 33 (44.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_553_135 49 (9.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_553_135 61 (25.5) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_553_135 62 (22.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_553_135 49 (9.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_553_135 47 (14.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_553_135 34 (17.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_553_135 42 (14.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_553_135 33 (18.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_553_135 33 (6.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_553_135 33 (18.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_553_135 44 (28.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_553_135 44 (16.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_553_135 38 (10.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_553_135 41 (13.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_553_135 35 (18.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_553_135 33 (6.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_553_135 33 (18.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_553_135 44 (28.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_553_135 44 (13.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_553_135 33 (18.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_553_135 38 (16.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_553_135 44 (6.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_553_135 33 (18.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_553_135 44 (28.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_553_135 44 (16.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_139_74 41 (19.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_139_74 41 (22.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_139_74 58 (26.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_139_74 46 (21.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_139_74 41 (22.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_139_74 44 (20.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_139_74 41 (22.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_139_74 58 (26.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_139_74 66 (25.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_139_74 41 (22.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_139_74 42 (25.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_139_74 42 (16.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_139_74 36 (34.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_139_74 39 (18.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_139_74 35 (11.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_139_74 39 (18.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_139_74 51 (46.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_139_74 38 (42.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_139_74 42 (9.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_139_74 39 (34.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_139_74 39 (14.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_139_74 35 (11.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_139_74 39 (18.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_139_74 51 (46.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_139_74 41 (38.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_139_74 39 (18.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_139_74 36 (32.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_139_74 36 (11.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_139_74 39 (18.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_139_74 51 (46.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_139_74 38 (42.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_108_33 41 (19.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_108_33 46 (2.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_108_33 67 (21.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_108_33 41 (13.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_108_33 46 (2.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_108_33 45 (23.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_108_33 46 (2.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_108_33 67 (21.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_108_33 64 (18.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_108_33 46 (2.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_108_33 40 (17.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_108_33 46 (16.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_108_33 44 (14.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_108_33 31 (10.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_108_33 29 (19.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_108_33 31 (10.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_108_33 44 (26.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_108_33 46 (12.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_108_33 43 (6.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_108_33 48 (21.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_108_33 31 (6.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_108_33 29 (19.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_108_33 31 (10.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_108_33 44 (26.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_108_33 51 (21.0) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_108_33 31 (10.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_108_33 40 (12.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_108_33 29 (15.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_108_33 31 (10.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_108_33 44 (26.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_108_33 46 (12.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_980_965 44 (30.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_980_965 38 (15.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_980_965 71 (15.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_980_965 48 (25.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_980_965 38 (15.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_980_965 36 (29.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_980_965 38 (15.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_980_965 71 (15.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_980_965 75 (25.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_980_965 38 (15.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_980_965 50 (12.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_980_965 45 (19.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_980_965 33 (37.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_980_965 35 (25.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_980_965 43 (15.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_980_965 35 (25.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_980_965 51 (37.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_980_965 39 (37.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_980_965 30 (16.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_980_965 36 (37.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_980_965 37 (25.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_980_965 43 (15.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_980_965 35 (25.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_980_965 51 (37.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_980_965 39 (37.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_980_965 35 (25.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_980_965 35 (29.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_980_965 44 (15.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_980_965 35 (25.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_980_965 51 (37.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_980_965 39 (37.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_981_857 35 (27.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_981_857 41 (26.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_981_857 66 (26.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_981_857 39 (21.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_981_857 41 (26.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_981_857 51 (19.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_981_857 41 (26.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_981_857 66 (26.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_981_857 63 (28.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_981_857 41 (26.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_981_857 47 (24.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_981_857 34 (25.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_981_857 34 (15.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_981_857 41 (16.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_981_857 30 (23.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_981_857 41 (16.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_981_857 51 (19.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_981_857 38 (15.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_981_857 34 (18.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_981_857 39 (20.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_981_857 41 (12.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_981_857 30 (23.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_981_857 41 (16.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_981_857 51 (19.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_981_857 41 (16.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_981_857 41 (16.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_981_857 39 (9.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_981_857 30 (19.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_981_857 41 (16.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_981_857 51 (19.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_981_857 38 (15.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_8_865 49 (14.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_8_865 39 (35.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_8_865 64 (35.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_8_865 45 (20.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_8_865 39 (35.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_8_865 40 (27.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_8_865 39 (35.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_8_865 64 (35.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_8_865 72 (20.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_8_865 39 (35.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_8_865 45 (15.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_8_865 40 (19.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_8_865 43 (18.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_8_865 32 (20.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_8_865 34 (14.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_8_865 32 (20.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_8_865 44 (20.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_8_865 39 (16.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_8_865 40 (21.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_8_865 38 (15.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_8_865 34 (20.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_8_865 34 (14.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_8_865 32 (20.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_8_865 44 (20.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_8_865 38 (19.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_8_865 32 (20.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_8_865 42 (12.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_8_865 34 (10.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_8_865 32 (20.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_8_865 44 (20.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_8_865 39 (16.5) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_292_484 37 (16.5) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_292_484 35 (22.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_292_484 58 (22.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_292_484 36 (24.0) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_292_484 35 (22.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_292_484 40 (31.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_292_484 35 (22.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_292_484 58 (22.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_292_484 61 (34.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_292_484 35 (22.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_292_484 37 (34.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_292_484 34 (16.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_292_484 33 (22.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_292_484 35 (10.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_292_484 30 (14.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_292_484 35 (10.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_292_484 53 (19.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_292_484 37 (22.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_292_484 29 (16.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_292_484 44 (19.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_292_484 35 (6.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_292_484 30 (14.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_292_484 35 (10.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_292_484 53 (19.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_292_484 47 (19.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_292_484 35 (10.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_292_484 31 (22.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_292_484 38 (16.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_292_484 35 (10.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_292_484 53 (19.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_292_484 37 (22.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_675_16 39 (17.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_675_16 56 (10.0) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_675_16 81 (10.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_675_16 39 (16.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_675_16 56 (10.0) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_675_16 43 (32.5) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_675_16 56 (10.0) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_675_16 81 (10.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_675_16 67 (26.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_675_16 56 (10.0) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_675_16 43 (26.0) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_675_16 43 (13.0) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_675_16 40 (17.0) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_675_16 33 (13.5) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_675_16 42 (11.0) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_675_16 33 (13.5) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_675_16 51 (17.5) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_675_16 41 (22.0) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_675_16 38 (13.0) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_675_16 43 (17.0) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_675_16 35 (13.5) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_675_16 42 (11.0) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_675_16 33 (13.5) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_675_16 51 (17.5) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_675_16 43 (21.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_675_16 33 (13.5) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_675_16 48 (15.0) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_675_16 42 (7.0) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_675_16 33 (13.5) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_675_16 51 (17.5) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_675_16 41 (22.0) +Illumina_Genomici_DNA_Adapters1_1 FC12044_91407_8_200_285_136 40 (21.0) +Illumina_Genomic_DNA_Adapters1_2 FC12044_91407_8_200_285_136 45 (17.5) +Illumina_Genomic_DNA_PCR_Primers1_1 FC12044_91407_8_200_285_136 65 (30.0) +Illumina_Genomic_DNA_PCR_Primers1_2 FC12044_91407_8_200_285_136 39 (16.5) +Illumina_Genomic_DNA_sequencing_primer FC12044_91407_8_200_285_136 45 (17.5) +Illumina_Paired_End_DNA_Adapters1_1 FC12044_91407_8_200_285_136 52 (7.0) +Illumina_Paired_End_DNA_Adapters1_2 FC12044_91407_8_200_285_136 45 (17.5) +Illumina_Paired_End_DNA_PCR_Primers1_1 FC12044_91407_8_200_285_136 65 (30.0) +Illumina_Paired_End_DNA_PCR_Primers1_2 FC12044_91407_8_200_285_136 61 (21.0) +Illumina_Paired_End_DNA_sequencing_primer_1 FC12044_91407_8_200_285_136 45 (17.5) +Illumina_Paired_End_DNA_sequencing_primer_2 FC12044_91407_8_200_285_136 48 (18.5) +Illumina_DpnII_Gex_Adapters1_1 FC12044_91407_8_200_285_136 32 (27.5) +Illumina_DpnII_Gex_Adapters1_2 FC12044_91407_8_200_285_136 31 (13.5) +Illumina_DpnII_Gex_Adapters2_1 FC12044_91407_8_200_285_136 43 (6.0) +Illumina_DpnII_Gex_Adapters2_2 FC12044_91407_8_200_285_136 35 (17.5) +Illumina_DpnII_Gex_PCR_Primer_1 FC12044_91407_8_200_285_136 43 (6.0) +Illumina_DpnII_Gex_PCR_Primer_2 FC12044_91407_8_200_285_136 46 (12.0) +Illumina_DpnII_Gex_sequencing_primer FC12044_91407_8_200_285_136 36 (17.5) +Illumina_NlaIII_Gex_Adapters1_1 FC12044_91407_8_200_285_136 31 (26.5) +Illumina_NlaIII_Gex_Adapters1_2 FC12044_91407_8_200_285_136 32 (14.5) +Illumina_NlaIII_Gex_Adapters2_1 FC12044_91407_8_200_285_136 43 (2.0) +Illumina_NlaIII_Gex_Adapters2_2 FC12044_91407_8_200_285_136 35 (17.5) +Illumina_NlaIII_Gex_PCR_Primer_1 FC12044_91407_8_200_285_136 43 (6.0) +Illumina_NlaIII_Gex_PCR_Primer_2 FC12044_91407_8_200_285_136 46 (12.0) +Illumina_NlaIII_Gex_sequencing_primer FC12044_91407_8_200_285_136 33 (15.5) +Illumina_Small_RNA_RT_Primer FC12044_91407_8_200_285_136 43 (6.0) +Illumina_Small_RNA_5p_Adapter FC12044_91407_8_200_285_136 44 (15.5) +Illumina_Small_RNA_3p_Adapter FC12044_91407_8_200_285_136 36 (17.5) +Illumina_Small_RNA_PCR_Primer_1 FC12044_91407_8_200_285_136 43 (6.0) +Illumina_Small_RNA_PCR_Primer_2 FC12044_91407_8_200_285_136 46 (12.0) +Illumina_Small_RNA_sequencing_primer FC12044_91407_8_200_285_136 36 (17.5) + +#--------------------------------------- +#---------------------------------------