Mercurial > repos > iuc > emboss_needleall
diff test-data/emboss_needleall_out.pair @ 0:76373359fce2 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/emboss commit 08f38b1f9b4241ba9037c64f732621efc628fd43
| author | iuc |
|---|---|
| date | Mon, 20 Jan 2025 16:21:51 +0000 |
| parents | |
| children |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/emboss_needleall_out.pair Mon Jan 20 16:21:51 2025 +0000 @@ -0,0 +1,18267 @@ +######################################## +# Program: needleall +# Rundate: Mon 16 Dec 2024 17:13:24 +# Commandline: needleall +# -asequence /tmp/saskia/tmpet25av5g/files/e/e/8/dataset_ee891bb1-6c31-453a-8e30-69544c761887.dat +# -bsequence /tmp/saskia/tmpet25av5g/files/0/7/0/dataset_07021fba-c6f2-478f-8665-0e6832dba409.dat +# -outfile /tmp/saskia/tmpet25av5g/job_working_directory/000/9/outputs/dataset_b9c62224-ff77-442f-a21f-1e30d4ffb6fc.dat +# -gapopen 10.0 +# -gapextend 0.5 +# -brief yes +# -aformat3 pair +# -auto +# -datafile EPAM30 +# -endopen 13.37 +# -endextend 2.5 +# -minscore 1.0 +# Align_format: pair +# Report_file: /tmp/saskia/tmpet25av5g/job_working_directory/000/9/outputs/dataset_b9c62224-ff77-442f-a21f-1e30d4ffb6fc.dat +######################################## + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 16/37 (43.2%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG---- 33 + |.| ||||| ||..|||..| .|| +FC12044_91407 1 GTT------AGCTC-----CCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACC--GAGATCTACACTCTTTCCCTACACGACGCT 48 + |.|| ||..||||| .||||.| ||. +FC12044_91407 1 ---GTTA--GCTCCCACCTTAAGATGT------TTA-------------- 25 + +Illumina_Geno 49 CTTCCGATCT 58 + +FC12044_91407 26 ---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 11/47 (23.4%) +# Similarity: 11/47 (23.4%) +# Gaps: 35/47 (74.5%) +# Score: 49.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACG--AGCTCTTCCGATCT----------- 34 + | ||||| || |.|| +FC12044_91407 1 -------------------GTTAGCTC--CC-ACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 33/45 (73.3%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCC------GAG------- 32 + ||| |||. || .|| +FC12044_91407 1 -------------GTT-AGCT------CCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACC--GAGATCTACACTCTTTCCCTACACGACGCT 48 + |.|| ||..||||| .||||.| ||. +FC12044_91407 1 ---GTTA--GCTCCCACCTTAAGATGT------TTA-------------- 25 + +Illumina_Pair 49 CTTCCGATCT 58 + +FC12044_91407 26 ---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 67 +# Identity: 15/67 (22.4%) +# Similarity: 15/67 (22.4%) +# Gaps: 48/67 (71.6%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGT---CTCGGCATTCCTGCTGAAC 47 + || ||| | +FC12044_91407 1 --------------------------GTTAGCTC---------------C 9 + +Illumina_Pair 48 CGCTCTTCCGATCT--- 61 + |.| |||..|||.| +FC12044_91407 10 CAC-CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + .| |||| || |||..|||.| +FC12044_91407 1 GTTAGC------TCCC-AC--------CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 24/43 (55.8%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 CGGT---CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT--- 37 + || ||| ||.| |||..|||.| +FC12044_91407 1 --GTTAGCTC---------------CCAC-CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 9/42 (21.4%) +# Similarity: 9/42 (21.4%) +# Gaps: 33/42 (78.6%) +# Score: 34.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGAAC---------------- 26 + || ||| ||| | +FC12044_91407 1 ----GT-------TAG--CTC----CCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 10/40 (25.0%) +# Similarity: 10/40 (25.0%) +# Gaps: 29/40 (72.5%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAG-TCCGAC------------- 26 + ||| || |||.|| +FC12044_91407 1 -----------GTT----AGCTCCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_DpnI 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 11/28 (39.3%) +# Similarity: 11/28 (39.3%) +# Gaps: 10/28 (35.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATG---CCGTCTTCTGCTTG---- 21 + ||..| ||..|||..| .|| +FC12044_91407 1 --GTTAGCTCCCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_DpnI 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA 44 + |.|| ||..||||| ||.||| ||| || +FC12044_91407 1 ---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 33/45 (73.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAG-TCCGACGATC------------ 32 + ||| || |||.|| | +FC12044_91407 1 -------------GTT----AGCTCCCAC---CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 7/37 (18.9%) +# Similarity: 7/37 (18.9%) +# Gaps: 28/37 (75.7%) +# Score: 31.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCTGAAC---------------- 21 + ||. |.||| | +FC12044_91407 1 -------GTT-AGCTC----CCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 26/40 (65.0%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAG-TCCGAC------ATG---- 29 + ||| || |||.|| ||| +FC12044_91407 1 -----------GTT----AGCTCCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 9/36 (25.0%) +# Similarity: 9/36 (25.0%) +# Gaps: 24/36 (66.7%) +# Score: 26.5 +# +# +#======================================= + +Illumina_NlaI 1 ---------CAAGC----AGAAGACGGCATACGANN 23 + || | |||.|. .|| +FC12044_91407 1 GTTAGCTCCCA--CCTTAAGATGT----TTA----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 11/28 (39.3%) +# Similarity: 11/28 (39.3%) +# Gaps: 10/28 (35.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATG---CCGTCTTCTGCTTG---- 21 + ||..| ||..|||..| .|| +FC12044_91407 1 --GTTAGCTCCCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_NlaI 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA 44 + |.|| ||..||||| ||.||| ||| || +FC12044_91407 1 ---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 29/43 (67.4%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAG-TCCGAC------ATG---- 32 + ||| || |||.|| ||| +FC12044_91407 1 --------------GTT----AGCTCCCACCTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_Smal 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 11/39 (28.2%) +# Similarity: 11/39 (28.2%) +# Gaps: 27/39 (69.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAG-TCCGACGATC------------ 26 + ||| || |||.|| | +FC12044_91407 1 -------GTT----AGCTCCCAC---CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 9/28 (32.1%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATG---CCGTCTTCTGCTTGT--- 22 + ||..| ||..|||..| .||| +FC12044_91407 1 --GTTAGCTCCCACCTTAAG-ATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 30.5 +# +# +#======================================= + +Illumina_Smal 1 --------CAAGCAGAAGACGGCATACGA------ 21 + | | || |.||.|| +FC12044_91407 1 GTTAGCTCC---C-----AC--CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGA-GTTCTACAGTCCGA 44 + |.|| ||..||||| ||.||| ||| || +FC12044_91407 1 ---GTTA--GCTCCCACC------TTAAGATGTT-TA-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_406_24 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 33/45 (73.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAG-TCCGACGATC------------ 32 + ||| || |||.|| | +FC12044_91407 1 -------------GTT----AGCTCCCAC---CTTAAGATGTTTA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATG-----CCGT-CTTCTGCTTG 33 + ||| |.|| ||.| |.||. ||| +FC12044_91407 1 -----------CTC-TGTGGCACCCCATCCCTCA-CTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 92.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCT---ACACTCTTTCCCTACACGACGC 47 + | ||| .|||.|..||||| | +FC12044_91407 1 ----------------C-----TCTGTGGCACCCCATCCCT--------C 21 + +Illumina_Geno 48 TCTTCCGATCT 58 + .||| +FC12044_91407 22 ACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 19/39 (48.7%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT----- 34 + |. |.|. ||| || |.|.||| || +FC12044_91407 1 CT--CTGT----GGC--AC---CCCATCC---CTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 21.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATG----CCGAG--------- 32 + | ||.| || ||.|. +FC12044_91407 1 -----------C---TCTG------TGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 92.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCT---ACACTCTTTCCCTACACGACGC 47 + | ||| .|||.|..||||| | +FC12044_91407 1 ----------------C-----TCTGTGGCACCCCATCCCT--------C 21 + +Illumina_Pair 48 TCTTCCGATCT 58 + .||| +FC12044_91407 22 ACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 16/64 (25.0%) +# Similarity: 16/64 (25.0%) +# Gaps: 42/64 (65.6%) +# Score: 86.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTC---GGCATTCCTGCTGAAC 47 + ||| ||||..||..| | +FC12044_91407 1 ----------------------------CTCTGTGGCACCCCATC----C 18 + +Illumina_Pair 48 CGCTCTTCCGATCT 61 + |.|.||| +FC12044_91407 19 CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTT-----CCCTACACGACGCTCTTCCGATCT 33 + ||||.| |||..| | |.|.||| +FC12044_91407 1 ---CTCTGTGGCACCCCATC-C--CTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 16/40 (40.0%) +# Similarity: 16/40 (40.0%) +# Gaps: 18/40 (45.0%) +# Score: 86.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTC---GGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + ||| ||||..||..| ||.|.||| +FC12044_91407 1 ----CTCTGTGGCACCCCATC----CCTCACTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 21/36 (58.3%) +# Score: 51.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAA--------CTCTGAAC-- 26 + | .||||.|.| ||| || +FC12044_91407 1 ------C--TCTGTGGCACCCCATCCCTC---ACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 8/40 (20.0%) +# Similarity: 8/40 (20.0%) +# Gaps: 29/40 (72.5%) +# Score: 43.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGT------CCGAC-------- 26 + || |.|| ||..| +FC12044_91407 1 --------------CT-CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 14/28 (50.0%) +# Similarity: 14/28 (50.0%) +# Gaps: 10/28 (35.7%) +# Score: 60.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATG-----CCGT-CTTCTGCTTG 21 + || |.|| ||.| |.||. ||| +FC12044_91407 1 CTC-TGTGGCACCCCATCCCTCA-CTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 15/52 (28.8%) +# Similarity: 15/52 (28.8%) +# Gaps: 35/52 (67.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC-----GGCGACC---ACCGACAGGTTCAGAGTTCTACAGTCC 42 + | ||| ||| .|| | ||| ||. +FC12044_91407 1 -------CTCTGTGGC-ACCCCATCC--C----TCA-----CTT------ 25 + +Illumina_DpnI 43 GA 44 + +FC12044_91407 26 -- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 51.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGT----CCGACGATC-------- 32 + || |.|| || |.||| +FC12044_91407 1 ----------------CT-CTGTGGCACC--CCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAA--------CTCTGAAC-- 21 + | .||||.|.| ||| || +FC12044_91407 1 -C--TCTGTGGCACCCCATCCCTC---ACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 16/35 (45.7%) +# Score: 44.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACA----GTCC--GACATG 29 + | ||.|.| .|| .||| .||.| +FC12044_91407 1 -C----TCTGTG----GCACCCCATCCCTCACTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 10/29 (34.5%) +# Score: 24.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCAT-----ACGANN 23 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 14/28 (50.0%) +# Similarity: 14/28 (50.0%) +# Gaps: 10/28 (35.7%) +# Score: 60.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATG-----CCGT-CTTCTGCTTG 21 + || |.|| ||.| |.||. ||| +FC12044_91407 1 CTC-TGTGGCACCCCATCCCTCA-CTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 15/52 (28.8%) +# Similarity: 15/52 (28.8%) +# Gaps: 35/52 (67.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC-----GGCGACC---ACCGACAGGTTCAGAGTTCTACAGTCC 42 + | ||| ||| .|| | ||| ||. +FC12044_91407 1 -------CTCTGTGGC-ACCCCATCC--C----TCA-----CTT------ 25 + +Illumina_NlaI 43 GA 44 + +FC12044_91407 26 -- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 29/43 (67.4%) +# Score: 46.5 +# +# +#======================================= + +Illumina_NlaI 1 -----------CCGACAGGTTCAGAGTTCTACAGTCCGACATG 32 + || || || | | || ||.| +FC12044_91407 1 CTCTGTGGCACCC--CA---TC------C--C--TC--ACTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 11/38 (28.9%) +# Similarity: 11/38 (28.9%) +# Gaps: 25/38 (65.8%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGT----CCGACGATC-------- 26 + || |.|| || |.||| +FC12044_91407 1 ----------CT-CTGTGGCACC--CCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 11/29 (37.9%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATG-----CCGT-CTTCTGCTTGT 22 + || |.|| ||.| |.||. ||| +FC12044_91407 1 CTC-TGTGGCACCCCATCCCTCA-CTT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 11/27 (40.7%) +# Similarity: 11/27 (40.7%) +# Gaps: 8/27 (29.6%) +# Score: 24.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCAT-----ACGA 21 + |. |.|..| ||..||| ||.. +FC12044_91407 1 CT--CTGTGGCACCCCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 15/52 (28.8%) +# Similarity: 15/52 (28.8%) +# Gaps: 35/52 (67.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC-----GGCGACC---ACCGACAGGTTCAGAGTTCTACAGTCC 42 + | ||| ||| .|| | ||| ||. +FC12044_91407 1 -------CTCTGTGGC-ACCCCATCC--C----TCA-----CTT------ 25 + +Illumina_Smal 43 GA 44 + +FC12044_91407 26 -- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_720_610 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGT----CCGACGATC-------- 32 + || |.|| || |.||| +FC12044_91407 1 ----------------CT-CTGTGGCACC--CCATCCCTCACTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 12/40 (30.0%) +# Similarity: 12/40 (30.0%) +# Gaps: 22/40 (55.0%) +# Score: 37.5 +# +# +#======================================= + +Illumina_Geno 1 GAT-------CGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + ||| |...|.| |||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAA---CGTA------------CATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 13/69 (18.8%) +# Similarity: 13/69 (18.8%) +# Gaps: 55/69 (79.7%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Geno 1 -----------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTA 39 + ||| |.||| |||| || +FC12044_91407 1 GATTTTTTAACAAT-AAACG---------------TACA---------TA 25 + +Illumina_Geno 40 CACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 31/45 (68.9%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Geno 1 ----------CAAGCAGAAGACG-GCATACGAGCTCTTCCGATCT 34 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 12/39 (30.8%) +# Similarity: 12/39 (30.8%) +# Gaps: 21/39 (53.8%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 GAT-------CGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + ||| | .|..|.| ||.|| .| +FC12044_91407 1 GATTTTTTAAC-AATAAAC-GTACA-----TA------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 13/69 (18.8%) +# Similarity: 13/69 (18.8%) +# Gaps: 55/69 (79.7%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Pair 1 -----------AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTA 39 + ||| |.||| |||| || +FC12044_91407 1 GATTTTTTAACAAT-AAACG---------------TACA---------TA 25 + +Illumina_Pair 40 CACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 72 +# Identity: 11/72 (15.3%) +# Similarity: 11/72 (15.3%) +# Gaps: 58/72 (80.6%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Pair 1 ----------CAAGCAGAAGACG-GCATACGAGATCGGTCTCGGCATTCC 39 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--------------------- 25 + +Illumina_Pair 40 TGCTGAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ---------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 33.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTTCCCTACAC----GACGCTCTTCCGATCT 33 + | |.||| || || .|||..|.| | +FC12044_91407 1 GA---TTTTT---TA-ACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 24/43 (55.8%) +# Score: 28.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAAC------CGCTCTTCCGATCT 37 + |.| .|| |.||| ||..|.| | +FC12044_91407 1 -GAT------TTT-----TTAACAATAAACGTACAT---A--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 15/33 (45.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGAAC------- 26 + ||| |.|..|||..|.||| +FC12044_91407 1 GAT--------TTTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 14/33 (42.4%) +# Similarity: 14/33 (42.4%) +# Gaps: 15/33 (45.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCT-ACAGT--CCG-AC--- 26 + |.|| ||.| |||.| .|| || +FC12044_91407 1 ---GATT-----TTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 6/37 (16.2%) +# Similarity: 6/37 (16.2%) +# Gaps: 28/37 (75.7%) +# Score: 26.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------------TCGTATGCCGTCTTCTGCTTG 21 + .|||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAACGTA------------CATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 55 +# Identity: 12/55 (21.8%) +# Similarity: 12/55 (21.8%) +# Gaps: 41/55 (74.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 -----------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG 39 + ||| |.|| ||.|| || +FC12044_91407 1 GATTTTTTAACAAT-AAAC---------------GTACA------TA--- 25 + +Illumina_DpnI 40 TCCGA 44 + +FC12044_91407 26 ----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 15/36 (41.7%) +# Score: 48.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCT-ACAGTCCGACGATC--- 32 + |.|| ||.| |||.| ..|||..| +FC12044_91407 1 -----GATT-----TTTTAACAAT-AAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 11/28 (39.3%) +# Similarity: 11/28 (39.3%) +# Gaps: 10/28 (35.7%) +# Score: 47.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCTGAAC------- 21 + ||.|.|..|||..|.||| +FC12044_91407 1 ---GATTTTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 16/33 (48.5%) +# Similarity: 16/33 (48.5%) +# Gaps: 12/33 (36.4%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCT-ACAGT--CCG-ACATG 29 + |.|| ||.| |||.| .|| ||||. +FC12044_91407 1 ---GATT-----TTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 20/34 (58.8%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAGAAGACG-GCATACGANN 23 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 6/37 (16.2%) +# Similarity: 6/37 (16.2%) +# Gaps: 28/37 (75.7%) +# Score: 26.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------------TCGTATGCCGTCTTCTGCTTG 21 + .|||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAACGTA------------CATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 55 +# Identity: 12/55 (21.8%) +# Similarity: 12/55 (21.8%) +# Gaps: 41/55 (74.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 -----------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG 39 + ||| |.|| ||.|| || +FC12044_91407 1 GATTTTTTAACAAT-AAAC---------------GTACA------TA--- 25 + +Illumina_NlaI 40 TCCGA 44 + +FC12044_91407 26 ----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 15/36 (41.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCT-ACAGT--CCG-ACATG 32 + |.|| ||.| |||.| .|| ||||. +FC12044_91407 1 ------GATT-----TTTTAACAATAAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 13/31 (41.9%) +# Similarity: 13/31 (41.9%) +# Gaps: 11/31 (35.5%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCT--ACAGTCCGACGATC--- 26 + ||.||.| |||.| ..|||..| +FC12044_91407 1 -----GATTTTTTAACAAT-AAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 6/38 (15.8%) +# Similarity: 6/38 (15.8%) +# Gaps: 29/38 (76.3%) +# Score: 26.5 +# +# +#======================================= + +Illumina_Smal 1 ----------------TCGTATGCCGTCTTCTGCTTGT 22 + .|||| |.|. +FC12044_91407 1 GATTTTTTAACAATAAACGTA------------CATA- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAGAAGACG-GCATACGA 21 + ||| .|.||| .|||| +FC12044_91407 1 GATTTTTTAACAA----TAAACGTACATA--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 55 +# Identity: 12/55 (21.8%) +# Similarity: 12/55 (21.8%) +# Gaps: 41/55 (74.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 -----------AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG 39 + ||| |.|| ||.|| || +FC12044_91407 1 GATTTTTTAACAAT-AAAC---------------GTACA------TA--- 25 + +Illumina_Smal 40 TCCGA 44 + +FC12044_91407 26 ----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_345_133 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 15/36 (41.7%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCT-ACAGTCCGACGATC--- 32 + |.|| ||.| |||.| ..|||..| +FC12044_91407 1 -----GATT-----TTTTAACAAT-AAACGTACATA 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 67.5 +# +# +#======================================= + +Illumina_Geno 1 GAT---CGGAAGAGCTCGTATGCCGTCTT---CTGCTTG 33 + |.| |.| ||| |||||| ||.| +FC12044_91407 1 GTTGCCCAG----GCT-------CGTCTTGAACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 16/61 (26.2%) +# Similarity: 16/61 (26.2%) +# Gaps: 39/61 (63.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCT-ACACGACGCTC 49 + . ||.|||. .|.|| || +FC12044_91407 1 -----------------------------G-TTGCCCAGGCTCG----TC 16 + +Illumina_Geno 50 TTCCGATCT-- 58 + || ||.|| +FC12044_91407 17 TT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 21/40 (52.5%) +# Score: 69.0 +# +# +#======================================= + +Illumina_Geno 1 ------CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + ||.|| .||.|.| ||.||| | +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 27/42 (64.3%) +# Score: 46.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTT---CAG------CAGGAA-TGCCGAG 32 + ||| ||| |..||| | || +FC12044_91407 1 -------------GTTGCCCAGGCTCGTCTTGAACT-CC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 16/61 (26.2%) +# Similarity: 16/61 (26.2%) +# Gaps: 39/61 (63.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCT-ACACGACGCTC 49 + . ||.|||. .|.|| || +FC12044_91407 1 -----------------------------G-TTGCCCAGGCTCG----TC 16 + +Illumina_Pair 50 TTCCGATCT-- 58 + || ||.|| +FC12044_91407 17 TT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 19/64 (29.7%) +# Similarity: 19/64 (29.7%) +# Gaps: 42/64 (65.6%) +# Score: 83.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGAT---CGGTCTCGGCATTCCTGCTGAAC 47 + |.| |.|.|||| | || ||||| +FC12044_91407 1 ---------------------GTTGCCCAGGCTCG---T--CT--TGAAC 22 + +Illumina_Pair 48 CGCTCTTCCGATCT 61 + ||| +FC12044_91407 23 ------TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-ACACGACGCTCTTCCGATCT-- 33 + .||.|||. .|.|| |||| ||.|| +FC12044_91407 1 -----GTTGCCCAGGCTCG----TCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 17/43 (39.5%) +# Similarity: 17/43 (39.5%) +# Gaps: 24/43 (55.8%) +# Score: 82.5 +# +# +#======================================= + +Illumina_Pair 1 ------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + |.|.|||| | || ||||| ||| +FC12044_91407 1 GTTGCCCAGGCTCG---T--CT--TGAAC------TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 14/35 (40.0%) +# Similarity: 14/35 (40.0%) +# Gaps: 19/35 (54.3%) +# Score: 77.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------GATCGTCGGACTGTAGAACTCTGAAC 26 + |.||||| |.|||||| | +FC12044_91407 1 GTTGCCCAGGCTCGTC------TTGAACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 63.0 +# +# +#======================================= + +Illumina_DpnI 1 -----ACAGGTTCAGAGTTCT---ACAGTCCGAC 26 + .||||.||. ||| || ||| +FC12044_91407 1 GTTGCCCAGGCTCG----TCTTGAAC--TCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 14/31 (45.2%) +# Similarity: 14/31 (45.2%) +# Gaps: 16/31 (51.6%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGC-------CGTCTT---CTGCTTG 21 + || ||| |||||| ||.| +FC12044_91407 1 --GT-TGCCCAGGCTCGTCTTGAACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 75.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCT---ACAGTCCGA 44 + |.|.| ||| |.| ||. ||| || ||| +FC12044_91407 1 ---GTTGC-----CCA--GGC----TCG----TCTTGAAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 68.5 +# +# +#======================================= + +Illumina_DpnI 1 -----CGACAGGTTCAGAGTTCT---ACAGTCCGACGATC 32 + | ||||.||. ||| || ||| +FC12044_91407 1 GTTGCC--CAGGCTCG----TCTTGAAC--TCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 61.0 +# +# +#======================================= + +Illumina_NlaI 1 -----------TCGGACTGTAGAACTCTGAAC 21 + || |.| |.|||||| | +FC12044_91407 1 GTTGCCCAGGCTC-GTC--TTGAACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 20/37 (54.1%) +# Score: 63.0 +# +# +#======================================= + +Illumina_NlaI 1 -----ACAGGTTCAGAGTTCT---ACAGTCCGACATG 29 + .||||.||. ||| || ||| +FC12044_91407 1 GTTGCCCAGGCTCG----TCTTGAAC--TCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 20/34 (58.8%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAGAAGACGGCATA-----CGANN 23 + ||.|| .||.|.|. | +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTTGAACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 14/31 (45.2%) +# Similarity: 14/31 (45.2%) +# Gaps: 16/31 (51.6%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGC-------CGTCTT---CTGCTTG 21 + || ||| |||||| ||.| +FC12044_91407 1 --GT-TGCCCAGGCTCGTCTTGAACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 75.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCT---ACAGTCCGA 44 + |.|.| ||| |.| ||. ||| || ||| +FC12044_91407 1 ---GTTGC-----CCA--GGC----TCG----TCTTGAAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 78.5 +# +# +#======================================= + +Illumina_NlaI 1 ----CCGACAGGTTCAGAGTTCT---ACAGTCCGACATG 32 + || ||||.||. ||| || ||| +FC12044_91407 1 GTTGCC--CAGGCTCG----TCTTGAAC--TCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 15/34 (44.1%) +# Similarity: 15/34 (44.1%) +# Gaps: 17/34 (50.0%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Smal 1 GTT---CAGAGT--TCT---ACAGTCCGACGATC 26 + ||| |||..| ||| || ||| +FC12044_91407 1 GTTGCCCAGGCTCGTCTTGAAC--TCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 17/32 (53.1%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGC-------CGTCTT---CTGCTTGT 22 + || ||| |||||| ||.| +FC12044_91407 1 --GT-TGCCCAGGCTCGTCTTGAACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 44.5 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAGAAGACGGCATACGA----- 21 + ||.|| .||.|.| || +FC12044_91407 1 GTTGCCCAGGC-----TCGTCTT--GAACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 75.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCT---ACAGTCCGA 44 + |.|.| ||| |.| ||. ||| || ||| +FC12044_91407 1 ---GTTGC-----CCA--GGC----TCG----TCTTGAAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_106_131 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Smal 1 -----CGACAGGTTCAGAGTTCT---ACAGTCCGACGATC 32 + | ||||.||. ||| || ||| +FC12044_91407 1 GTTGCC--CAGGCTCG----TCTTGAAC--TCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 18/37 (48.6%) +# Similarity: 18/37 (48.6%) +# Gaps: 16/37 (43.2%) +# Score: 55.0 +# +# +#======================================= + +Illumina_Geno 1 -GATCGGAAGAGCTCGTATGCCGT--C-TTCTGCTTG 33 + ||| .|||| |||.| || | |.||| +FC12044_91407 1 TGAT-TGAAG-----GTAGG--GTAGCATACTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Geno 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATC---TA-CACTCTTTCCCTACACGACG 46 + ||||. || ||.|. || || |||. | +FC12044_91407 1 --TGATT----GA------AGGTAGGGTAGCA---------TACT-G--- 25 + +Illumina_Geno 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 27/43 (62.8%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Geno 1 -----CAAGCAGAAGACG---GCATAC-GAGCTCTTCCGATCT 34 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG-------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Geno 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 19/35 (54.3%) +# Similarity: 19/35 (54.3%) +# Gaps: 13/35 (37.1%) +# Score: 56.5 +# +# +#======================================= + +Illumina_Pair 1 -GATCGGAAG--AGCGGTTCAGCAGGAATGCCGAG 32 + ||| .|||| || ||| ||| ||.|.| +FC12044_91407 1 TGAT-TGAAGGTAG-GGT--AGC----ATACTG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Pair 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATC---TA-CACTCTTTCCCTACACGACG 46 + ||||. || ||.|. || || |||. | +FC12044_91407 1 --TGATT----GA------AGGTAGGGTAGCA---------TACT-G--- 25 + +Illumina_Pair 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 19/62 (30.6%) +# Similarity: 19/62 (30.6%) +# Gaps: 38/62 (61.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGA-AGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG 49 + .|| .||.|| || | ||| .||||. ||| +FC12044_91407 1 -----TGATTGAAGG--TA-G-----GGT---AGCATA-CTG-------- 25 + +Illumina_Pair 50 CTCTTCCGATCT 61 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 6/50 (12.0%) +# Similarity: 6/50 (12.0%) +# Gaps: 42/50 (84.0%) +# Score: 18.0 +# +# +#======================================= + +Illumina_Pair 1 -----------------ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| |||. | +FC12044_91407 1 TGATTGAAGGTAGGGTAGCA---------TACT-G--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 10/49 (20.4%) +# Similarity: 10/49 (20.4%) +# Gaps: 36/49 (73.5%) +# Score: 38.0 +# +# +#======================================= + +Illumina_Pair 1 ------------CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + .||| .||||. ||| +FC12044_91407 1 TGATTGAAGGTAGGGT---AGCATA-CTG-------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 14/31 (45.2%) +# Similarity: 14/31 (45.2%) +# Gaps: 11/31 (35.5%) +# Score: 49.0 +# +# +#======================================= + +Illumina_DpnI 1 -----GATCGTCGGACTGTAGAACTCTGAAC 26 + ||..||.|| ||||.|..||| +FC12044_91407 1 TGATTGAAGGTAGG---GTAGCATACTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 50.5 +# +# +#======================================= + +Illumina_DpnI 1 ------ACAGGTTCAGAGTT-C-TACAGTCCGAC 26 + | |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 30.0 +# +# +#======================================= + +Illumina_DpnI 1 -------TCGTATGCCGT--C-TTCTGCTTG 21 + ..|||.| || | |.||| +FC12044_91407 1 TGATTGAAGGTAGG--GTAGCATACTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 19/46 (41.3%) +# Similarity: 19/46 (41.3%) +# Gaps: 23/46 (50.0%) +# Score: 66.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTT-C-TACAGTCCGA 44 + ||||. || |||| ||.||. | |||.| +FC12044_91407 1 --TGATT----GA--------AGGT--AGGGTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 48.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAGGTTCAGAGTT-C-TACAGTCCGACGATC 32 + .|| |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 ----------TCGGACTGTAGAACTCTGAAC 21 + |.|| ||||.|..||| +FC12044_91407 1 TGATTGAAGGTAGG---GTAGCATACTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 20/37 (54.1%) +# Score: 50.5 +# +# +#======================================= + +Illumina_NlaI 1 ------ACAGGTTCAGAGTT-C-TACAGTCCGACATG 29 + | |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 16/32 (50.0%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 -----CAAGCAGAAGACG---GCATAC-GANN 23 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 -------TCGTATGCCGT--C-TTCTGCTTG 21 + ..|||.| || | |.||| +FC12044_91407 1 TGATTGAAGGTAGG--GTAGCATACTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 19/46 (41.3%) +# Similarity: 19/46 (41.3%) +# Gaps: 23/46 (50.0%) +# Score: 66.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTT-C-TACAGTCCGA 44 + ||||. || |||| ||.||. | |||.| +FC12044_91407 1 --TGATT----GA--------AGGT--AGGGTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 21/39 (53.8%) +# Score: 46.0 +# +# +#======================================= + +Illumina_NlaI 1 -----CCGACAGGTTCAGAGTT-C-TACAGTCCGACATG 32 + || |||| ||.||. | |||.| +FC12044_91407 1 TGATT--GA-AGGT--AGGGTAGCATACTG--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 11/37 (29.7%) +# Similarity: 11/37 (29.7%) +# Gaps: 23/37 (62.2%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 ---------GTTCAGAGTT-C-TACAGTCCGACGATC 26 + || ||.||. | |||.| +FC12044_91407 1 TGATTGAAGGT--AGGGTAGCATACTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 17/32 (53.1%) +# Score: 30.0 +# +# +#======================================= + +Illumina_Smal 1 -------TCGTATGCCGT--C-TTCTGCTTGT 22 + ..|||.| || | |.||| +FC12044_91407 1 TGATTGAAGGTAGG--GTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 -----CAAGCAGAAGACG---GCATAC-GA 21 + .||| |.|| | |||||| | +FC12044_91407 1 TGATTGAAG--GTAG--GGTAGCATACTG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 19/46 (41.3%) +# Similarity: 19/46 (41.3%) +# Gaps: 23/46 (50.0%) +# Score: 66.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTT-C-TACAGTCCGA 44 + ||||. || |||| ||.||. | |||.| +FC12044_91407 1 --TGATT----GA--------AGGT--AGGGTAGCATACTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_916_471 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAGGTTCAGAGTT-C-TACAGTCCGACGATC 32 + .|| |||| ||.||. | |||.| +FC12044_91407 1 TGATTGA-AGGT--AGGGTAGCATACTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTAT---GCCG-----TCTTCTGCTTG 33 + ||||..|| ||.| .| ||| +FC12044_91407 1 ----------GCTCCAATAGCGCAGAGGAAAC--CTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCA------CCGAGATCTACACTCTTTCCCTACACGA 44 + || .||| |.|||. |.| |||. +FC12044_91407 1 ---------GC-TCCAATAGCGCAGAGG---AAA-------CCTG----- 25 + +Illumina_Geno 45 CGCTCTTCCGATCT 58 + +FC12044_91407 26 -------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 27/43 (62.8%) +# Score: 58.5 +# +# +#======================================= + +Illumina_Geno 1 ----CAA----GCAGAAGACGGCATACGAGCTCTTCCGATCT- 34 + ||| |||||.| |.|| || +FC12044_91407 1 GCTCCAATAGCGCAGAGG-----AAAC-------------CTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 20/33 (60.6%) +# Similarity: 20/33 (60.6%) +# Gaps: 9/33 (27.3%) +# Score: 68.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCC-GAG 32 + |.|| .||.|||| ||| |||||. || | +FC12044_91407 1 GCTC-CAATAGCG---CAG-AGGAAA-CCTG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCA------CCGAGATCTACACTCTTTCCCTACACGA 44 + || .||| |.|||. |.| |||. +FC12044_91407 1 ---------GC-TCCAATAGCGCAGAGG---AAA-------CCTG----- 25 + +Illumina_Pair 45 CGCTCTTCCGATCT 58 + +FC12044_91407 26 -------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 14/69 (20.3%) +# Similarity: 14/69 (20.3%) +# Gaps: 52/69 (75.4%) +# Score: 64.0 +# +# +#======================================= + +Illumina_Pair 1 ----CAA----GCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGC 42 + ||| |||||.| |..|||| +FC12044_91407 1 GCTCCAATAGCGCAGAGG------------------------AAACCTG- 25 + +Illumina_Pair 43 TGAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 9/48 (18.8%) +# Similarity: 9/48 (18.8%) +# Gaps: 38/48 (79.2%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTCCGAT--------------CT- 33 + || |||.|| || +FC12044_91407 1 --------------------GC---TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 16/44 (36.4%) +# Similarity: 16/44 (36.4%) +# Gaps: 26/44 (59.1%) +# Score: 61.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATT--CCTGCTG----AACC-GCTCTTCCGATCT 37 + | ||| ||.| | ||.| |||| | +FC12044_91407 1 -G--CTC--CAATAGC--GCAGAGGAAACCTG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 19/35 (54.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAG---------AACTCTGAAC 26 + |.|| |..||| ||| ||| +FC12044_91407 1 GCTC------CAATAGCGCAGAGGAAAC-CTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 12/35 (34.3%) +# Similarity: 12/35 (34.3%) +# Gaps: 19/35 (54.3%) +# Score: 31.5 +# +# +#======================================= + +Illumina_DpnI 1 ------ACAG-GTTCAGAG--TTCTACAGTCCGAC 26 + |.|| | ||||| ..| |.| +FC12044_91407 1 GCTCCAATAGCG--CAGAGGAAAC--CTG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 34.5 +# +# +#======================================= + +Illumina_DpnI 1 --TCGTAT---GCCG-----TCTTCTGCTTG 21 + ||..|| ||.| .| ||| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAAC--CTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 25/47 (53.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAG-GTTCAGAG--TTCTACAGTCCGA 44 + . |.||.|.|| | ||||| ..| |.| +FC12044_91407 1 ------------G-CTCCAATAGCG--CAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 13/39 (33.3%) +# Similarity: 13/39 (33.3%) +# Gaps: 21/39 (53.8%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAG-GTTCAGAG--TTCTACAGTCCGACGATC 32 + |.|.|| | ||||| ..| |.| +FC12044_91407 1 GCTCCAATAGCG--CAGAGGAAAC--CTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 --TCGGACTGTAG---------AACTCTGAAC 21 + || |..||| ||| ||| +FC12044_91407 1 GCTC---CAATAGCGCAGAGGAAAC-CTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 12/38 (31.6%) +# Similarity: 12/38 (31.6%) +# Gaps: 22/38 (57.9%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTC-CG--------ACATG 29 + | ||.||.|. || ||.|| +FC12044_91407 1 -----------G--CTCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 12/34 (35.3%) +# Similarity: 12/34 (35.3%) +# Gaps: 20/34 (58.8%) +# Score: 53.5 +# +# +#======================================= + +Illumina_NlaI 1 ----CAA----GCAGAAGA---CGGCATACGANN 23 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 34.5 +# +# +#======================================= + +Illumina_NlaI 1 --TCGTAT---GCCG-----TCTTCTGCTTG 21 + ||..|| ||.| .| ||| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAAC--CTG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_NlaI 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 25/47 (53.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAG-GTTCAGAG--TTCTACAGTCCGA 44 + . |.||.|.|| | ||||| ..| |.| +FC12044_91407 1 ------------G-CTCCAATAGCG--CAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 15/36 (41.7%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAG-GTTCAGAGTTCTACAGTCCGACATG 32 + ||.|.|| | |||||. |..|| || +FC12044_91407 1 GCTCCAATAGCG--CAGAGG-----AAACC----TG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_Smal 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 9/39 (23.1%) +# Similarity: 9/39 (23.1%) +# Gaps: 27/39 (69.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGTCCGA---CG-------ATC--- 26 + .| |||.| || |.| +FC12044_91407 1 ------------GC--TCCAATAGCGCAGAGGAAACCTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 17/32 (53.1%) +# Score: 34.5 +# +# +#======================================= + +Illumina_Smal 1 --TCGTAT---GCCG-----TCTTCTGCTTGT 22 + ||..|| ||.| .| ||| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 53.5 +# +# +#======================================= + +Illumina_Smal 1 ----CAA----GCAGAAGA---CGGCATACGA 21 + ||| |||||.|| |.| +FC12044_91407 1 GCTCCAATAGCGCAGAGGAAACCTG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 25/47 (53.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAG-GTTCAGAG--TTCTACAGTCCGA 44 + . |.||.|.|| | ||||| ..| |.| +FC12044_91407 1 ------------G-CTCCAATAGCG--CAGAGGAAAC--CTG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_57_85 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 13/39 (33.3%) +# Similarity: 13/39 (33.3%) +# Gaps: 21/39 (53.8%) +# Score: 39.5 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAG-GTTCAGAG--TTCTACAGTCCGACGATC 32 + |.|.|| | ||||| ..| |.| +FC12044_91407 1 GCTCCAATAGCG--CAGAGGAAAC--CTG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 8/50 (16.0%) +# Similarity: 8/50 (16.0%) +# Gaps: 42/50 (84.0%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG----------------- 33 + | ||||||| +FC12044_91407 1 ---------------------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 10/70 (14.3%) +# Similarity: 10/70 (14.3%) +# Gaps: 57/70 (81.4%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT-C 49 + ||| | +FC12044_91407 1 ---------------------------------------------GCTGC 5 + +Illumina_Geno 50 TTCCGA-TCT---------- 58 + ||..|| .|| +FC12044_91407 6 TTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 27/43 (62.8%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Geno 1 ----C-----AAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + | |.||.|| ||||. ||| +FC12044_91407 1 GCTGCTTGGGAGGCTGA----GGCAG--GAG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 13/35 (37.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Pair 1 ---GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + |.|.||.|| ||.|. .||||||. +FC12044_91407 1 GCTGCTTGGGAG-GCTGA--GGCAGGAG------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 10/70 (14.3%) +# Similarity: 10/70 (14.3%) +# Gaps: 57/70 (81.4%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT-C 49 + ||| | +FC12044_91407 1 ---------------------------------------------GCTGC 5 + +Illumina_Pair 50 TTCCGA-TCT---------- 58 + ||..|| .|| +FC12044_91407 6 TTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 13/70 (18.6%) +# Similarity: 13/70 (18.6%) +# Gaps: 54/70 (77.1%) +# Score: 47.0 +# +# +#======================================= + +Illumina_Pair 1 ----C-----AAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTG 41 + | |.||.|| ||||. ||| +FC12044_91407 1 GCTGCTTGGGAGGCTGA----GGCAG--GAG------------------- 25 + +Illumina_Pair 42 CTGAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 -------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 10/45 (22.2%) +# Similarity: 10/45 (22.2%) +# Gaps: 32/45 (71.1%) +# Score: 32.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCT-CTTCCGA-TCT---------- 33 + ||| |||..|| .|| +FC12044_91407 1 --------------------GCTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 13/45 (28.9%) +# Similarity: 13/45 (28.9%) +# Gaps: 28/45 (62.2%) +# Score: 38.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCT---GAACCGCTCTTCCGA-TCT---- 37 + . ||||| ||. ||| || .|. +FC12044_91407 1 ----------G---CTGCTTGGGAG--GCT-----GAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 10/39 (25.6%) +# Similarity: 10/39 (25.6%) +# Gaps: 27/39 (69.2%) +# Score: 40.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACT-------CTGA-AC----- 26 + .||| || |||| .| +FC12044_91407 1 ---------GCTG-----CTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 13/32 (40.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTT------CAGAGTTCTACAGTCCGAC 26 + .|.|.|| |.||| .||| ||. +FC12044_91407 1 GCTGCTTGGGAGGCTGAG----GCAG---GAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTCTTCTGCTTG----------------- 21 + | ||||||| +FC12044_91407 1 ---------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + |.|.| ||| || |.|||| || +FC12044_91407 1 ---GCTGCTTGGGAGGCTGA-----GGCAGG---AG-------------- 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 40.0 +# +# +#======================================= + +Illumina_DpnI 1 -CGAC-----AGGTTCAGAGTTCTACAGTCCGACGATC 32 + |..| ||| |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGG--CTGAG----GCAG---GA-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 40.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACT-------CTGA-AC----- 21 + .||| || |||| .| +FC12044_91407 1 ----GCTG-----CTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 14/35 (40.0%) +# Similarity: 14/35 (40.0%) +# Gaps: 16/35 (45.7%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTT------CAGAGTTCTACAGTCCGACATG 29 + .|.|.|| |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGGCTGAG----GCAG---GA---G 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 28/38 (73.7%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------C--AAGCAGAAGACGGCATACGANN 23 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTCTTCTGCTTG----------------- 21 + | ||||||| +FC12044_91407 1 ---------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + |.|.| ||| || |.|||| || +FC12044_91407 1 ---GCTGCTTGGGAGGCTGA-----GGCAGG---AG-------------- 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 14/38 (36.8%) +# Similarity: 14/38 (36.8%) +# Gaps: 19/38 (50.0%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTT------CAGAGTTCTACAGTCCGACATG 32 + .|.|.|| |.||| .||| || | +FC12044_91407 1 ---GCTGCTTGGGAGGCTGAG----GCAG---GA---G 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 30.0 +# +# +#======================================= + +Illumina_Smal 1 ---G-TT------CAGAGTTCTACAGTCCGACGATC 26 + | || |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGGCTGAG----GCAG---GA-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 9/38 (23.7%) +# Similarity: 9/38 (23.7%) +# Gaps: 29/38 (76.3%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCTTCTGCTTG------T---------- 22 + | ||||||| | +FC12044_91407 1 ---------G----CTGCTTGGGAGGCTGAGGCAGGAG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 26/36 (72.2%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 -------------C--AAGCAGAAGACGGCATACGA 21 + | |.||||.|| +FC12044_91407 1 GCTGCTTGGGAGGCTGAGGCAGGAG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + |.|.| ||| || |.|||| || +FC12044_91407 1 ---GCTGCTTGGGAGGCTGA-----GGCAGG---AG-------------- 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_10_437 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 40.0 +# +# +#======================================= + +Illumina_Smal 1 -CGAC-----AGGTTCAGAGTTCTACAGTCCGACGATC 32 + |..| ||| |.||| .||| || | +FC12044_91407 1 GCTGCTTGGGAGG--CTGAG----GCAG---GA-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 26/42 (61.9%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGT---ATGC------CGTCTTCTGCTTG 33 + |||.|| | ||.| ||.|| +FC12044_91407 1 -------AGACCT--TTGGATACAATGAACGACT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Geno 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 17/66 (25.8%) +# Similarity: 17/66 (25.8%) +# Gaps: 49/66 (74.2%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TAC-----AC 42 + ||| | |||| ||| || +FC12044_91407 1 -------------------AGA-C------CTTT---GGATACAATGAAC 21 + +Illumina_Geno 43 GACGCTCTTCCGATCT 58 + || || +FC12044_91407 22 GA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 17/41 (41.5%) +# Similarity: 17/41 (41.5%) +# Gaps: 23/41 (56.1%) +# Score: 63.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGAC----GGCATAC---GAGCTCTTCCGATCT 34 + |||| || |||| ||. ||| || +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAA------CGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Geno 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 17/37 (45.9%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGC----GGTTCAGCAGGAATGCCGAG- 32 + |||.| |.|.|| |.||| |||. +FC12044_91407 1 -------AGACCTTTGGATACA--ATGAA---CGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Pair 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 17/66 (25.8%) +# Similarity: 17/66 (25.8%) +# Gaps: 49/66 (74.2%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TAC-----AC 42 + ||| | |||| ||| || +FC12044_91407 1 -------------------AGA-C------CTTT---GGATACAATGAAC 21 + +Illumina_Pair 43 GACGCTCTTCCGATCT 58 + || || +FC12044_91407 22 GA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 17/66 (25.8%) +# Similarity: 17/66 (25.8%) +# Gaps: 46/66 (69.7%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGAC----GGCATACGA-GATCGGTCTCGGCATTCCTGCTGA 45 + |||| || ||||.| ||.| |.|| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAAC-GACT--------------- 25 + +Illumina_Pair 46 ACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 15/43 (34.9%) +# Similarity: 15/43 (34.9%) +# Gaps: 28/43 (65.1%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Pair 1 --ACACTCTTTCCC---TAC-----ACGACGCTCTTCCGATCT 33 + || |||| ||| |||| || +FC12044_91407 1 AGAC---CTTT---GGATACAATGAACGA--CT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 12/45 (26.7%) +# Similarity: 12/45 (26.7%) +# Gaps: 28/45 (62.2%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCC------TGC--TGAACCGCTCTTCCGATCT 37 + |..|| |.| |||||..|| +FC12044_91407 1 ----------AGACCTTTGGATACAATGAACGACT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 13/32 (40.6%) +# Score: 62.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGA-CTGTAGA-ACTCTGAAC---- 26 + .|| ||.|.|| ||..||||| +FC12044_91407 1 -------AGACCTTTGGATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 53.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTC-------TACA--GTCCGAC- 26 + ||| | |||| |..|||| +FC12044_91407 1 --------AGA---CCTTTGGATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 ------TCGTATGC------CGTCTTCTGCTTG 21 + |.|.||.| ||.|| +FC12044_91407 1 AGACCTTTGGATACAATGAACGACT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 56.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA--GTCCGA-- 44 + . |||| ||..|| |||| |..||| +FC12044_91407 1 ---------A-GACC---------TTTGGA----TACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTC-------TACAGTCCG-ACGATC- 32 + ||| | ||||.| | |||| | +FC12044_91407 1 ----------AGA---CCTTTGGATACAAT--GAACGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 62.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGA-CTGTAGA-ACTCTGAAC---- 21 + .|| ||.|.|| ||..||||| +FC12044_91407 1 --AGACCTTTGGATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 13/38 (34.2%) +# Similarity: 13/38 (34.2%) +# Gaps: 22/38 (57.9%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTC-------TACA--GTCCGACATG 29 + ||| | |||| |..||||. +FC12044_91407 1 --------AGA---CCTTTGGATACAATGAACGACT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 20/34 (58.8%) +# Score: 46.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC----GGCATACGANN------- 23 + |||| || ||||.|.. +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_NlaI 1 ------TCGTATGC------CGTCTTCTGCTTG 21 + |.|.||.| ||.|| +FC12044_91407 1 AGACCTTTGGATACAATGAACGACT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA--GTCCGA-- 44 + . |||| ||..|| |||| |..||| +FC12044_91407 1 ---------A-GACC---------TTTGGA----TACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 17/37 (45.9%) +# Score: 60.5 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAGGTTCAGAGTTCTACA--GTCCGACATG 32 + || ||..|| |||| |..||||. +FC12044_91407 1 AGACC------TTTGGA----TACAATGAACGACT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 19/35 (54.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTC-------TACAGTCCG-ACGATC- 26 + ||| | ||||.| | |||| | +FC12044_91407 1 ----AGA---CCTTTGGATACAAT--GAACGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 21/34 (61.8%) +# Score: 39.5 +# +# +#======================================= + +Illumina_Smal 1 ------TCGTATGC------CGTCTTCTGCTTGT 22 + |.|.||.| ||.|| +FC12044_91407 1 AGACCTTTGGATACAATGAACGACT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 22/34 (64.7%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC----GGCATACGA--------- 21 + |||| || ||||.| +FC12044_91407 1 --------AGACCTTTGG-ATACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 56.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACA--GTCCGA-- 44 + . |||| ||..|| |||| |..||| +FC12044_91407 1 ---------A-GACC---------TTTGGA----TACAATGAACGACT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_154_436 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTC-------TACAGTCCG-ACGATC- 32 + ||| | ||||.| | |||| | +FC12044_91407 1 ----------AGA---CCTTTGGATACAAT--GAACGA-CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 30/44 (68.2%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Geno 1 -----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + || |.||.|| ||||| +FC12044_91407 1 AGGGAATTTTAGA---GGAGGGC-----TGCCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 15/62 (24.2%) +# Similarity: 15/62 (24.2%) +# Gaps: 41/62 (66.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 ----AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + |||..|| |.| |||..||.| || +FC12044_91407 1 AGGGAATTTTA--GAG------GAGGGCTGC--------------CG--- 25 + +Illumina_Geno 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 19/39 (48.7%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCAT-----ACGAGCTCTTCCGATCT 34 + || ||.|| |.|||..||.||| +FC12044_91407 1 --------AG--GGAATTTTAGAGGAGGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 19/38 (50.0%) +# Score: 61.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGG---TTCAGCAGGAA---TGCCGAG 32 + || || ||.|| ||||. ||||| +FC12044_91407 1 ---------AG-GGAATTTTAG-AGGAGGGCTGCCG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 15/62 (24.2%) +# Similarity: 15/62 (24.2%) +# Gaps: 41/62 (66.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Pair 1 ----AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + |||..|| |.| |||..||.| || +FC12044_91407 1 AGGGAATTTTA--GAG------GAGGGCTGC--------------CG--- 25 + +Illumina_Pair 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 20/61 (32.8%) +# Similarity: 20/61 (32.8%) +# Gaps: 36/61 (59.0%) +# Score: 56.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + || ||.||...||| ||. || |||| ||| +FC12044_91407 1 --------AG--GGAATTTTAGA--GGA---GG-------GCTG--CCG- 25 + +Illumina_Pair 51 TCTTCCGATCT 61 + +FC12044_91407 26 ----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 18/38 (47.4%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT 33 + | ||| ||.|.|| |..||.||| +FC12044_91407 1 AGGGAA-----TTT---TAGAGGA-GGGCTGCCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 14/46 (30.4%) +# Similarity: 14/46 (30.4%) +# Gaps: 30/46 (65.2%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCT---------GCTGAACCGCTCTTCCGATCT 37 + .| ||.||| | |||| ||| +FC12044_91407 1 -AG----GGAATT--TTAGAGGAGGGCTG--CCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 9/41 (22.0%) +# Similarity: 9/41 (22.0%) +# Gaps: 31/41 (75.6%) +# Score: 28.5 +# +# +#======================================= + +Illumina_DpnI 1 --------------GATCGTCGGACTGTAGAACTCTGAAC- 26 + || ||.||| | | +FC12044_91407 1 AGGGAATTTTAGAGGA-----GGGCTG-----C------CG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTA--------CAGTCCGAC 26 + ||| ||.||.|| |.| ||| +FC12044_91407 1 --AGG----GAATTTTAGAGGAGGGCTG-CCG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 34.5 +# +# +#======================================= + +Illumina_DpnI 1 --------TCGTA---------TGCCGTCTTCTGCTTG 21 + | || ||||| +FC12044_91407 1 AGGGAATTT--TAGAGGAGGGCTGCCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 14/52 (26.9%) +# Similarity: 14/52 (26.9%) +# Gaps: 35/52 (67.3%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTA--------CAGTCC 42 + ||| ||.||.|| |.| || +FC12044_91407 1 ---------------------AGG----GAATTTTAGAGGAGGGCTG-CC 24 + +Illumina_DpnI 43 GA 44 + | +FC12044_91407 25 G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTA--------CAGTCCGACGATC 32 + ||| ||.||.|| |.| ||| +FC12044_91407 1 ----AGG----GAATTTTAGAGGAGGGCTG-CCG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 22.5 +# +# +#======================================= + +Illumina_NlaI 1 ------TCGGACTGTAGA-----ACT-CTGAAC- 21 + | |.|||| .|| | | +FC12044_91407 1 AGGGAAT-----TTTAGAGGAGGGCTGC----CG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 20/37 (54.1%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTA--------CAGTCCGACATG 29 + ||| ||.||.|| |.| ||| +FC12044_91407 1 --AGG----GAATTTTAGAGGAGGGCTG-CCG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 12/30 (40.0%) +# Score: 35.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAG-------CAGAAGACGGCATACGANN 23 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 8/38 (21.1%) +# Similarity: 8/38 (21.1%) +# Gaps: 30/38 (78.9%) +# Score: 34.5 +# +# +#======================================= + +Illumina_NlaI 1 --------TCGTA---------TGCCGTCTTCTGCTTG 21 + | || ||||| +FC12044_91407 1 AGGGAATTT--TAGAGGAGGGCTGCCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 14/52 (26.9%) +# Similarity: 14/52 (26.9%) +# Gaps: 35/52 (67.3%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTA--------CAGTCC 42 + ||| ||.||.|| |.| || +FC12044_91407 1 ---------------------AGG----GAATTTTAGAGGAGGGCTG-CC 24 + +Illumina_NlaI 43 GA 44 + | +FC12044_91407 25 G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTA--------CAGTCCGACATG 32 + ||| ||.||.|| |.| ||| +FC12044_91407 1 -----AGG----GAATTTTAGAGGAGGGCTG-CCG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Smal 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 21/36 (58.3%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCA--GAGTTCTA--------CAGTCCGACGATC 26 + | ||.||.|| |.| ||| +FC12044_91407 1 ----AGGGAATTTTAGAGGAGGGCTG-CCG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 8/39 (20.5%) +# Similarity: 8/39 (20.5%) +# Gaps: 31/39 (79.5%) +# Score: 34.5 +# +# +#======================================= + +Illumina_Smal 1 --------TCGTA---------TGCCGTCTTCTGCTTGT 22 + | || ||||| +FC12044_91407 1 AGGGAATTT--TAGAGGAGGGCTGCCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Smal 1 CAAG-------CAGAAGACGGCATACGA 21 + || .|||.||.|||...|| +FC12044_91407 1 --AGGGAATTTTAGAGGAGGGCTGCCG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 52 +# Identity: 14/52 (26.9%) +# Similarity: 14/52 (26.9%) +# Gaps: 35/52 (67.3%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTA--------CAGTCC 42 + ||| ||.||.|| |.| || +FC12044_91407 1 ---------------------AGG----GAATTTTAGAGGAGGGCTG-CC 24 + +Illumina_Smal 43 GA 44 + | +FC12044_91407 25 G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_336_64 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTA--------CAGTCCGACGATC 32 + ||| ||.||.|| |.| ||| +FC12044_91407 1 ----AGG----GAATTTTAGAGGAGGGCTG-CCG------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 28/43 (65.1%) +# Score: 50.0 +# +# +#======================================= + +Illumina_Geno 1 ----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + |.||.| ||| | ||| || +FC12044_91407 1 TCTCCATGTTGGTCAG----GCT-G------GTC-TC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 65.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTT--TCCCTACACGACGCT 48 + |||.|| |.|| || |.||| +FC12044_91407 1 ----------------------TCTCCA-TGTTGGTC--------AGGCT 19 + +Illumina_Geno 49 CTTCCGATCT- 58 + |.||| +FC12044_91407 20 -----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 13/44 (29.5%) +# Similarity: 13/44 (29.5%) +# Gaps: 29/44 (65.9%) +# Score: 53.5 +# +# +#======================================= + +Illumina_Geno 1 ----CAAG-----CAGAAGACGGCATACGAGCTCTTCCGATCT- 34 + ||.| ||| ||| |.||| +FC12044_91407 1 TCTCCATGTTGGTCAG--------------GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Pair 1 ----------GATCGGAAGAGC-GGT-TCAGCAGGAATGCCGAG 32 + |.||.| || ||| || +FC12044_91407 1 TCTCCATGTTGGTCAG----GCTGGTCTC--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 65.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTT--TCCCTACACGACGCT 48 + |||.|| |.|| || |.||| +FC12044_91407 1 ----------------------TCTCCA-TGTTGGTC--------AGGCT 19 + +Illumina_Pair 49 CTTCCGATCT- 58 + |.||| +FC12044_91407 20 -----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 16/66 (24.2%) +# Similarity: 16/66 (24.2%) +# Gaps: 46/66 (69.7%) +# Score: 81.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCAT-----TCCTGCTGA 45 + |||| ||| ||..||||. +FC12044_91407 1 ---------------------------TCTC--CATGTTGGTCAGGCTGG 21 + +Illumina_Pair 46 ACCGCTCTTCCGATCT 61 + . ||| +FC12044_91407 22 T---CTC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCT-----ACACGACGCTCTTCCGATCT- 33 + || |||.| .|| | ||| |.||| +FC12044_91407 1 ----TC--TCCATGTTGGTCA-G--GCT-----GGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 16/42 (38.1%) +# Similarity: 16/42 (38.1%) +# Gaps: 22/42 (52.4%) +# Score: 81.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCAT-----TCCTGCTGAACCGCTCTTCCGATCT 37 + |||| ||| ||..||||.. ||| +FC12044_91407 1 ---TCTC--CATGTTGGTCAGGCTGGT---CTC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 15/33 (45.5%) +# Score: 64.0 +# +# +#======================================= + +Illumina_DpnI 1 -------GATCGTCGGACTGTAGAACTCTGAAC 26 + |.|.|||.|.|| |..||| +FC12044_91407 1 TCTCCATGTTGGTCAGGCT---GGTCTC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 10/38 (26.3%) +# Similarity: 10/38 (26.3%) +# Gaps: 25/38 (65.8%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACA-----GTCCGAC------- 26 + |||.|| |||.|.| +FC12044_91407 1 -------------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 46.0 +# +# +#======================================= + +Illumina_DpnI 1 --TC---GTATG--CCGTCT--TCTGCTTG 21 + || || || |.|.|| ||| | +FC12044_91407 1 TCTCCATGT-TGGTCAGGCTGGTCT-C--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_DpnI 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 14/50 (28.0%) +# Similarity: 14/50 (28.0%) +# Gaps: 31/50 (62.0%) +# Score: 59.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACC------GACAGGTTCAGAGTTCTACAGTCCGA 44 + . |.|| |.|||| |.|. ||| | +FC12044_91407 1 ------------T-CTCCATGTTGGTCAGG--CTGG--TCT-C------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 64.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACA-----GTCCGAC-GATC-- 32 + |||.|| |||.|.| |.|| +FC12044_91407 1 ---------------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 12/28 (42.9%) +# Similarity: 12/28 (42.9%) +# Gaps: 10/28 (35.7%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 --TCGGAC-TGTAGAACT--CTGAAC-- 21 + || | |||.|..|. |||..| +FC12044_91407 1 TCTC---CATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 12/39 (30.8%) +# Similarity: 12/39 (30.8%) +# Gaps: 24/39 (61.5%) +# Score: 56.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACA-----GTCCGACATG----- 29 + |||.|| |||.|.| || +FC12044_91407 1 -------------TCTCCATGTTGGTCAGGC-TGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 8/36 (22.2%) +# Similarity: 8/36 (22.2%) +# Gaps: 24/36 (66.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------CAAGCAGAAGACGGCATACGANN 23 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 46.0 +# +# +#======================================= + +Illumina_NlaI 1 --TC---GTATG--CCGTCT--TCTGCTTG 21 + || || || |.|.|| ||| | +FC12044_91407 1 TCTCCATGT-TGGTCAGGCTGGTCT-C--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_NlaI 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 14/50 (28.0%) +# Similarity: 14/50 (28.0%) +# Gaps: 31/50 (62.0%) +# Score: 59.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACC------GACAGGTTCAGAGTTCTACAGTCCGA 44 + . |.|| |.|||| |.|. ||| | +FC12044_91407 1 ------------T-CTCCATGTTGGTCAGG--CTGG--TCT-C------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 ---CC------GACAGGTTCAGAGTTCTACAGTCCGACATG 32 + || |.|||| |.|. ||| | +FC12044_91407 1 TCTCCATGTTGGTCAGG--CTGG--TCT-C----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Smal 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 13/34 (38.2%) +# Similarity: 13/34 (38.2%) +# Gaps: 17/34 (50.0%) +# Score: 64.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACA-----GTCCGAC-GATC-- 26 + |||.|| |||.|.| |.|| +FC12044_91407 1 ---------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 11/29 (37.9%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCT---TCT-GCTTGT--- 22 + || | ||.|.| ||. |||.|| +FC12044_91407 1 TC-T---CCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 8/34 (23.5%) +# Similarity: 8/34 (23.5%) +# Gaps: 22/34 (64.7%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Smal 1 -------------CAAGCAGAAGACGGCATACGA 21 + ||.||.| |.| .| +FC12044_91407 1 TCTCCATGTTGGTCAGGCTG--GTC-----TC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 50 +# Identity: 14/50 (28.0%) +# Similarity: 14/50 (28.0%) +# Gaps: 31/50 (62.0%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACC------GACAGGTTCAGAGTTCTACAGTCCGA 44 + . |.|| |.|||| |.|. ||| | +FC12044_91407 1 ------------T-CTCCATGTTGGTCAGG--CTGG--TCT-C------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_620_233 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 64.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACA-----GTCCGAC-GATC-- 32 + |||.|| |||.|.| |.|| +FC12044_91407 1 ---------------TCTCCATGTTGGTCAGGCTGGTCTC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 20/39 (51.3%) +# Score: 57.0 +# +# +#======================================= + +Illumina_Geno 1 -GA---TCGGAAGAGCTCGTATG--CCGTCTTCTGCTTG 33 + || ||| ||| ||.|.| |||.| +FC12044_91407 1 TGAACGTCG--AGA---CGCAAGGCCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 13/68 (19.1%) +# Similarity: 13/68 (19.1%) +# Gaps: 53/68 (77.9%) +# Score: 70.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGA----------CCACCGAGATCTACACTCTTTCCCTAC 40 + ||| |||.||| ||.|| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_Geno 41 ACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 16/38 (42.1%) +# Similarity: 16/38 (42.1%) +# Gaps: 17/38 (44.7%) +# Score: 51.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAA-GACGGCATACG---AGCTCTTCCGATCT 34 + .||| |.|| |.||| .|| |||. | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGC----CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 15/36 (41.7%) +# Score: 56.0 +# +# +#======================================= + +Illumina_Pair 1 -GA---TCGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + || ||| ||| || || |||...||| +FC12044_91407 1 TGAACGTCG--AGA-CG---CA--AGGCCCGCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 13/68 (19.1%) +# Similarity: 13/68 (19.1%) +# Gaps: 53/68 (77.9%) +# Score: 70.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGA----------CCACCGAGATCTACACTCTTTCCCTAC 40 + ||| |||.||| ||.|| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_Pair 41 ACGACGCTCTTCCGATCT 58 + +FC12044_91407 26 ------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 21/61 (34.4%) +# Similarity: 21/61 (34.4%) +# Gaps: 36/61 (59.0%) +# Score: 85.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + .|| ||| .||||| || |..||| |||| +FC12044_91407 1 -----TGA--ACG----TCGAGA-CG--CAAGGC------------CCGC 24 + +Illumina_Pair 51 TCTTCCGATCT 61 + | +FC12044_91407 25 -C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-----GACGCT---CTTCCGATCT 33 + |..|| |||||. | |||. | +FC12044_91407 1 ------------TGAACGTCGAGACGCAAGGC--CCGC-C- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 15/45 (33.3%) +# Similarity: 15/45 (33.3%) +# Gaps: 28/45 (62.2%) +# Score: 61.0 +# +# +#======================================= + +Illumina_Pair 1 ----CGGTCTCG----GCATTCCTGCTGAACCGCTCTTCCGATCT 37 + || ||| |||. .|| |||| | +FC12044_91407 1 TGAACG---TCGAGACGCAA---GGC----CCGC-C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 13/32 (40.6%) +# Score: 62.5 +# +# +#======================================= + +Illumina_DpnI 1 -GATCGTCGGACTGTAGAACTCTGAAC----- 26 + ||.||||| || ||.|....| +FC12044_91407 1 TGAACGTCG------AG-ACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 15/31 (48.4%) +# Similarity: 15/31 (48.4%) +# Gaps: 11/31 (35.5%) +# Score: 48.5 +# +# +#======================================= + +Illumina_DpnI 1 ---ACAGGTTCAGAGTTCTACA--GTCCGAC 26 + ||. || ||| .| .|| |.|||.| +FC12044_91407 1 TGAACG---TC-GAG-AC-GCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_DpnI 1 ------TCGT-----ATG--CCGTCTTCTGCTTG 21 + |||. |.| |||.| +FC12044_91407 1 TGAACGTCGAGACGCAAGGCCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 71.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGC--GAC-CA----CCGACAGGTTCAGAGTTCTACAGTCCG 43 + ||| |||.| ||| || |||.| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 54.5 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAGGTTCAGAGTTCTAC---AGTCCGACGATC- 32 + ||.| ||| || ||.|| || | +FC12044_91407 1 TGAACGTC-------GAG----ACGCAAGGCC--CG--CC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 10/35 (28.6%) +# Similarity: 10/35 (28.6%) +# Gaps: 24/35 (68.6%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAAC-TCTGA-AC------------ 21 + .|||| || || || +FC12044_91407 1 ---------TGAACGTC-GAGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 15/34 (44.1%) +# Similarity: 15/34 (44.1%) +# Gaps: 14/34 (41.2%) +# Score: 48.5 +# +# +#======================================= + +Illumina_NlaI 1 ---ACAGGTTCAGAGTTCTACA--GTCCGACATG 29 + ||. || ||| .| .|| |.|||.| +FC12044_91407 1 TGAACG---TC-GAG-AC-GCAAGGCCCGCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 18/33 (54.5%) +# Score: 35.0 +# +# +#======================================= + +Illumina_NlaI 1 -------CAA---GCAGAAGACGGCATACGANN 23 + |.| ||| |.|.|.|| | +FC12044_91407 1 TGAACGTCGAGACGCA-AGGCCCGC---C---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_NlaI 1 ------TCGT-----ATG--CCGTCTTCTGCTTG 21 + |||. |.| |||.| +FC12044_91407 1 TGAACGTCGAGACGCAAGGCCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 71.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGC--GAC-CA----CCGACAGGTTCAGAGTTCTACAGTCCG 43 + ||| |||.| ||| || |||.| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 17/37 (45.9%) +# Score: 44.5 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAGGTTCAGAGTTCTACA--GTCCGACATG 32 + .||.| ||| .| .|| |.|||.| +FC12044_91407 1 TGAACGTC-------GAG-AC-GCAAGGCCCGCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 19/35 (54.3%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Smal 1 -----GTTCAGAGTTCTAC---AGTCCGACGATC- 26 + | || ||| || ||.|| || | +FC12044_91407 1 TGAACG-TC-GAG----ACGCAAGGCC--CG--CC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 23/35 (65.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_Smal 1 ------TCGT-----ATG--CCGTCTTCTGCTTGT 22 + |||. |.| |||.| +FC12044_91407 1 TGAACGTCGAGACGCAAGGCCCGCC---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 35.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAA-GACGGCATACG-A--------- 21 + .||| |.|| |.||| | +FC12044_91407 1 -----TGAACGTCG--AGACGCAAGGCCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 71.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGC--GAC-CA----CCGACAGGTTCAGAGTTCTACAGTCCG 43 + ||| |||.| ||| || |||.| +FC12044_91407 1 --TGA-ACGTCGAGACGCAAGGCCCGCC---------------------- 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_902_349 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 54.5 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAGGTTCAGAGTTCTAC---AGTCCGACGATC- 32 + ||.| ||| || ||.|| || | +FC12044_91407 1 TGAACGTC-------GAG----ACGCAAGGCC--CG--CC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 30/44 (68.2%) +# Score: 35.0 +# +# +#======================================= + +Illumina_Geno 1 -----------GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + | |||..|.|| ||| | +FC12044_91407 1 CTGTCCCCACGG--CGGGGGGGC--------------CTG---G 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 15/66 (22.7%) +# Similarity: 15/66 (22.7%) +# Gaps: 49/66 (74.2%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC----- 45 + ||.|||| ||||.| +FC12044_91407 1 ------------------------------CTGTCCC--CACGGCGGGGG 18 + +Illumina_Geno 46 -GCTCTTCCGATCT-- 58 + || || +FC12044_91407 19 GGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 12/42 (28.6%) +# Similarity: 12/42 (28.6%) +# Gaps: 25/42 (59.5%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Geno 1 ------CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-- 34 + | | |||||....|.|| || +FC12044_91407 1 CTGTCCC---C-----ACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 15/36 (41.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGT----TCAGCAGGAATGCCGAG 32 + |.|| .|.||.||...|||..| +FC12044_91407 1 -----------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 15/66 (22.7%) +# Similarity: 15/66 (22.7%) +# Gaps: 49/66 (74.2%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC----- 45 + ||.|||| ||||.| +FC12044_91407 1 ------------------------------CTGTCCC--CACGGCGGGGG 18 + +Illumina_Pair 46 -GCTCTTCCGATCT-- 58 + || || +FC12044_91407 19 GGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 67 +# Identity: 17/67 (25.4%) +# Similarity: 17/67 (25.4%) +# Gaps: 48/67 (71.6%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Pair 1 ------CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTG 44 + | | |||| ||.| || ||| ||| +FC12044_91407 1 CTGTCCC---C-----ACGG----CGGG---GG----GGC-------CTG 24 + +Illumina_Pair 45 AACCGCTCTTCCGATCT 61 + . +FC12044_91407 25 G---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 84.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC------GCTCTTCCGATCT-- 33 + ||.|||| ||||.| || || +FC12044_91407 1 -----CTGTCCC--CACGGCGGGGGGGC---------CTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 13/47 (27.7%) +# Similarity: 13/47 (27.7%) +# Gaps: 32/47 (68.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Pair 1 CGGT---CTCGGCATT-------CCTGCTGAACCGCTCTTCCGATCT 37 + |.|| |.|||| |||| | +FC12044_91407 1 CTGTCCCCACGGC---GGGGGGGCCTG--G----------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 13/32 (40.6%) +# Score: 38.0 +# +# +#======================================= + +Illumina_DpnI 1 GATC-GT-----CGGACTGTAGAACTCTGAAC 26 + | || ||| |.|..|..| |||. +FC12044_91407 1 ---CTGTCCCCACGG-CGGGGGGGC-CTGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 7/42 (16.7%) +# Similarity: 7/42 (16.7%) +# Gaps: 33/42 (78.6%) +# Score: 44.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGTC----CGAC------------ 26 + |.||| ||.| +FC12044_91407 1 -----------------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_DpnI 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 29.0 +# +# +#======================================= + +Illumina_DpnI 1 ---TCGTATGCCGTCTTCTGCTTG----------- 21 + || || | .|.|| | +FC12044_91407 1 CTGTC-----CC--C-ACGGC--GGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_DpnI 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 16/47 (34.0%) +# Similarity: 16/47 (34.0%) +# Gaps: 25/47 (53.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGC-GACCACCGACAGGTTCAGAGTTCTACAGTCC--GA 44 + | |.|| || ||.| |.|.| .|.|| | +FC12044_91407 1 ----------CTGTCC-CC-ACGG---CGGGG------GGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 43.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGT-CCGACGATC------------ 32 + |.|| ||.|||. | +FC12044_91407 1 -------------------CTGTCCCCACGG-CGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 10/29 (34.5%) +# Similarity: 10/29 (34.5%) +# Gaps: 12/29 (41.4%) +# Score: 36.0 +# +# +#======================================= + +Illumina_NlaI 1 --------TCGGACTGTAGAACTCTGAAC 21 + .||| |.|..|..| |||. +FC12044_91407 1 CTGTCCCCACGG-CGGGGGGGC-CTGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 9/42 (21.4%) +# Similarity: 9/42 (21.4%) +# Gaps: 30/42 (71.4%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCC------------GACATG- 29 + |.|||| |.|.|| +FC12044_91407 1 -----------------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 14/31 (45.2%) +# Score: 32.5 +# +# +#======================================= + +Illumina_NlaI 1 -------CA-AGCAGAAGACGGCATACGANN 23 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 24/35 (68.6%) +# Score: 29.0 +# +# +#======================================= + +Illumina_NlaI 1 ---TCGTATGCCGTCTTCTGCTTG----------- 21 + || || | .|.|| | +FC12044_91407 1 CTGTC-----CC--C-ACGGC--GGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_NlaI 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 16/47 (34.0%) +# Similarity: 16/47 (34.0%) +# Gaps: 25/47 (53.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGC-GACCACCGACAGGTTCAGAGTTCTACAGTCC--GA 44 + | |.|| || ||.| |.|.| .|.|| | +FC12044_91407 1 ----------CTGTCC-CC-ACGG---CGGGG------GGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAGTCC------------GACATG- 32 + |.|||| |.|.|| +FC12044_91407 1 --------------------CTGTCCCCACGGCGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_Smal 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 9/39 (23.1%) +# Similarity: 9/39 (23.1%) +# Gaps: 27/39 (69.2%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGT-CCGACGATC------------ 26 + |.|| ||.|||. | +FC12044_91407 1 -------------CTGTCCCCACGG-CGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 10/31 (32.3%) +# Similarity: 10/31 (32.3%) +# Gaps: 15/31 (48.4%) +# Score: 27.5 +# +# +#======================================= + +Illumina_Smal 1 ---TCGTATGCCGTCTTCTG------CTTGT 22 + || ||..|..|.| ||.| +FC12044_91407 1 CTGTC-----CCCACGGCGGGGGGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 11/29 (37.9%) +# Similarity: 11/29 (37.9%) +# Gaps: 12/29 (41.4%) +# Score: 32.5 +# +# +#======================================= + +Illumina_Smal 1 -------CA-AGCAGAAGACGGCATACGA 21 + || .||.|..| |||.|. | +FC12044_91407 1 CTGTCCCCACGGCGGGGG--GGCCTG-G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 16/47 (34.0%) +# Similarity: 16/47 (34.0%) +# Gaps: 25/47 (53.2%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGC-GACCACCGACAGGTTCAGAGTTCTACAGTCC--GA 44 + | |.|| || ||.| |.|.| .|.|| | +FC12044_91407 1 ----------CTGTCC-CC-ACGG---CGGGG------GGGCCTGG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_40_618 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 9/45 (20.0%) +# Similarity: 9/45 (20.0%) +# Gaps: 33/45 (73.3%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGT-CCGACGATC------------ 32 + |.|| ||.|||. | +FC12044_91407 1 -------------------CTGTCCCCACGG-CGGGGGGGCCTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 12/35 (34.3%) +# Score: 79.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGCC--GTCTTCTGCTTG 33 + ||.|..|||.||..|| |.||| || +FC12044_91407 1 -----GATGTACTCTTACACCCAGACTT-TG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 93.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + |||.|||.|| ||||| +FC12044_91407 1 --------------------GATGTACTCT-------TACAC-------- 15 + +Illumina_Geno 51 TCCGATCT--- 58 + ||...|| +FC12044_91407 16 -CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 14/41 (34.1%) +# Similarity: 14/41 (34.1%) +# Gaps: 23/41 (56.1%) +# Score: 67.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTT----CCGATCT--- 34 + ||.| || ||||| ||...|| +FC12044_91407 1 ------GATG------TA----CTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Geno 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 12/39 (30.8%) +# Similarity: 12/39 (30.8%) +# Gaps: 21/39 (53.8%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTC-AGCAGGAATGCCGAG------ 32 + ||.|..| || ..|| ||.|| +FC12044_91407 1 -----GATGTAC---TCTTACA------CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 93.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + |||.|||.|| ||||| +FC12044_91407 1 --------------------GATGTACTCT-------TACAC-------- 15 + +Illumina_Pair 51 TCCGATCT--- 58 + ||...|| +FC12044_91407 16 -CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 65 +# Identity: 19/65 (29.2%) +# Similarity: 19/65 (29.2%) +# Gaps: 44/65 (67.7%) +# Score: 69.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGT-CTCGGCATTCCTGCTGAACCG 49 + ||| || ||| ||.| ||| +FC12044_91407 1 ---------------------GAT--GTACTC----TTAC------ACC- 16 + +Illumina_Pair 50 CTCTTCCGATCT--- 61 + |.|| || +FC12044_91407 17 -----CAGA-CTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 24/41 (58.5%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Pair 1 -----ACACTCTTTCCCTACACGACGCTCTTCCGATCT--- 33 + ||.|| ||||| ||...|| +FC12044_91407 1 GATGTACTCT-------TACAC---------CCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 13/44 (29.5%) +# Similarity: 13/44 (29.5%) +# Gaps: 26/44 (59.1%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGCTCTT----CCGATCT--- 37 + |.||.| ||||| ||...|| +FC12044_91407 1 ----------------GATGTA---CTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 13/37 (35.1%) +# Similarity: 13/37 (35.1%) +# Gaps: 23/37 (62.2%) +# Score: 57.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGA-AC---------- 26 + || ||| |||||.| || +FC12044_91407 1 --------GA-TGT---ACTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 21/36 (58.3%) +# Score: 63.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGA-GTTC---TACAGTCC--GAC---- 26 + || ||.| |||| || ||| +FC12044_91407 1 ---------GATGTACTCTTACA--CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 55.5 +# +# +#======================================= + +Illumina_DpnI 1 -------TCGTATGCC--GTCTTCTGCTTG 21 + ||.||..|| |.||| || +FC12044_91407 1 GATGTACTCTTACACCCAGACTT-TG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGAC---CACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + .||| ||| .| |||| ||||.|| |. +FC12044_91407 1 GATG-TAC----TCTTACACC--------CAGACTT-TG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 58.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGA-GTTC---TACAGTCC--GAC---GATC 32 + || ||.| |||| || ||| | +FC12044_91407 1 -----------GATGTACTCTTACA--CCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 13/32 (40.6%) +# Similarity: 13/32 (40.6%) +# Gaps: 18/32 (56.2%) +# Score: 57.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCTGA-AC---------- 21 + || ||| |||||.| || +FC12044_91407 1 ---GA-TGT---ACTCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 18/36 (50.0%) +# Score: 65.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGA-GTTC---TACAGTCC--GAC-ATG 29 + || ||.| |||| || ||| .|| +FC12044_91407 1 ---------GATGTACTCTTACA--CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 10/35 (28.6%) +# Similarity: 10/35 (28.6%) +# Gaps: 22/35 (62.9%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCATAC-----------GANN 23 + ||.| || .|.||| | +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 55.5 +# +# +#======================================= + +Illumina_NlaI 1 -------TCGTATGCC--GTCTTCTGCTTG 21 + ||.||..|| |.||| || +FC12044_91407 1 GATGTACTCTTACACCCAGACTT-TG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGAC---CACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + .||| ||| .| |||| ||||.|| |. +FC12044_91407 1 GATG-TAC----TCTTACACC--------CAGACTT-TG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 65.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGA-GTTC---TACAGTCC--GAC-ATG 32 + || ||.| |||| || ||| .|| +FC12044_91407 1 ------------GATGTACTCTTACA--CCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 19/35 (54.3%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Smal 1 ---GTTCAGAGTTC-TACAGTCC--GAC---GATC 26 + ||.| || |||| || ||| | +FC12044_91407 1 GATGTAC-----TCTTACA--CCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 15/31 (48.4%) +# Score: 55.5 +# +# +#======================================= + +Illumina_Smal 1 -------TCGTATGCC--GTCTTCTGCTTGT 22 + ||.||..|| |.||| || +FC12044_91407 1 GATGTACTCTTACACCCAGACTT-TG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAG-ACGGCATAC-----GA----- 21 + ||.| || .|.||| || +FC12044_91407 1 ------GATGTAC-TCTTACACCCAGACTTTG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGAC---CACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + .||| ||| .| |||| ||||.|| |. +FC12044_91407 1 GATG-TAC----TCTTACACC--------CAGACTT-TG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_83_511 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 25/41 (61.0%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGA-GTTC---TACAGTCC--GAC---GATC 32 + || ||.| |||| || ||| | +FC12044_91407 1 -----------GATGTACTCTTACA--CCCAGACTTTG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 14/42 (33.3%) +# Similarity: 14/42 (33.3%) +# Gaps: 26/42 (61.9%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Geno 1 GATC---GGAAGA-----GCTCGTATGCC-GTCTTCTGCTTG 33 + || ||..|| |||| || || +FC12044_91407 1 --TCAAGGGTGGATCTTGGCTC-----CCAGT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Geno 1 --AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT 48 + ||.|.| .||||| |. ||| +FC12044_91407 1 TCAAGGGT-------------GGATCT------------TG------GCT 19 + +Illumina_Geno 49 CTTCCGATCT 58 + | ||..| +FC12044_91407 20 C--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 11/35 (31.4%) +# Score: 63.0 +# +# +#======================================= + +Illumina_Geno 1 -CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + |||| .|..|| .|.| .|||| ||..| +FC12044_91407 1 TCAAG-GGTGGA--TCTT---GGCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Geno 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 13/35 (37.1%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCA--GCAGGAATGCCGAG- 32 + || |||.|.||.||. ||. ||.|| +FC12044_91407 1 --TC--AAGGGTGGATCTTGGCT------CCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 17/60 (28.3%) +# Similarity: 17/60 (28.3%) +# Gaps: 37/60 (61.7%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Pair 1 --AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCT 48 + ||.|.| .||||| |. ||| +FC12044_91407 1 TCAAGGGT-------------GGATCT------------TG------GCT 19 + +Illumina_Pair 49 CTTCCGATCT 58 + | ||..| +FC12044_91407 20 C--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 18/62 (29.0%) +# Similarity: 18/62 (29.0%) +# Gaps: 38/62 (61.3%) +# Score: 74.0 +# +# +#======================================= + +Illumina_Pair 1 -CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCG 49 + |||| |.|.|.|||.||| ||| |.|. +FC12044_91407 1 TCAAG-----------------GGTGGATCTTGGC--TCC--CAGT---- 25 + +Illumina_Pair 50 CTCTTCCGATCT 61 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 22/40 (55.0%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Pair 1 ACA-------CTCTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| .|||| | |||| ||..| +FC12044_91407 1 TCAAGGGTGGATCTT---------G--GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 19/41 (46.3%) +# Similarity: 19/41 (46.3%) +# Gaps: 20/41 (48.8%) +# Score: 65.0 +# +# +#======================================= + +Illumina_Pair 1 -C---GGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT 37 + | ||| || || || || |||| ||..| +FC12044_91407 1 TCAAGGGT---GG-AT--CT--TG----GCTC--CCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTC--GGACTGTAGAACT---CTGAAC----- 26 + || || ||.||.|| || | +FC12044_91407 1 -----TCAAGG---GTGGATCTTGGCT---CCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 58.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTC-AGAGT---TCTA------CAGTCCGAC 26 + || ||.|| |||. |||| +FC12044_91407 1 ------TCAAGGGTGGATCTTGGCTCCCAGT----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_DpnI 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 38.5 +# +# +#======================================= + +Illumina_DpnI 1 TC--GTATGCCG-TCTT--CTGCTTG- 21 + || |..|| | |||| ||.|..| +FC12044_91407 1 TCAAGGGTG--GATCTTGGCTCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_DpnI 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 13/54 (24.1%) +# Similarity: 13/54 (24.1%) +# Gaps: 39/54 (72.2%) +# Score: 58.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCTA------CAGT 40 + || ||.|| |||. |||| +FC12044_91407 1 -------------------------TCAAGGGTGGATCTTGGCTCCCAGT 25 + +Illumina_DpnI 41 CCGA 44 + +FC12044_91407 26 ---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 60.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTC-AGAGT---TCTA--CAGTCCGACGATC 32 + || ||.|| |||. | ||| |..| +FC12044_91407 1 --------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 13/31 (41.9%) +# Similarity: 13/31 (41.9%) +# Gaps: 16/31 (51.6%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 TC--GGACTGTAGAACT---CTGAAC----- 21 + || || ||.||.|| || | +FC12044_91407 1 TCAAGG---GTGGATCTTGGCT---CCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 18/36 (50.0%) +# Score: 60.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTC-AGAGT---TCTA--CAGTCCGACA-TG 29 + || ||.|| |||. | ||| || | +FC12044_91407 1 ------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 10/29 (34.5%) +# Score: 38.0 +# +# +#======================================= + +Illumina_NlaI 1 -CAAGCAGAAGA-C--GGCATAC--GANN 23 + |||| .|..|| | ||| |.| |. +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 38.5 +# +# +#======================================= + +Illumina_NlaI 1 TC--GTATGCCG-TCTT--CTGCTTG- 21 + || |..|| | |||| ||.|..| +FC12044_91407 1 TCAAGGGTG--GATCTTGGCTCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_NlaI 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 13/54 (24.1%) +# Similarity: 13/54 (24.1%) +# Gaps: 39/54 (72.2%) +# Score: 58.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCTA------CAGT 40 + || ||.|| |||. |||| +FC12044_91407 1 -------------------------TCAAGGGTGGATCTTGGCTCCCAGT 25 + +Illumina_NlaI 41 CCGA 44 + +FC12044_91407 26 ---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 60.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTC-AGAGT---TCTA--CAGTCCGACA-TG 32 + || ||.|| |||. | ||| || | +FC12044_91407 1 ---------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Smal 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 15/32 (46.9%) +# Similarity: 15/32 (46.9%) +# Gaps: 13/32 (40.6%) +# Score: 60.5 +# +# +#======================================= + +Illumina_Smal 1 GTTC-AGAGT---TCTA--CAGTCCGACGATC 26 + || ||.|| |||. | ||| |..| +FC12044_91407 1 --TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 15/27 (55.6%) +# Similarity: 15/27 (55.6%) +# Gaps: 7/27 (25.9%) +# Score: 45.5 +# +# +#======================================= + +Illumina_Smal 1 TC--GTATGCCG-TCTT--CTGCTTGT 22 + || |..|| | |||| ||.|..|| +FC12044_91407 1 TCAAGGGTG--GATCTTGGCTCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Smal 1 -CAAGCAGAAGA-C--GGCATACGA-- 21 + |||| .|..|| | ||| |.|.| +FC12044_91407 1 TCAAG-GGTGGATCTTGGC-TCCCAGT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 13/54 (24.1%) +# Similarity: 13/54 (24.1%) +# Gaps: 39/54 (72.2%) +# Score: 58.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTC-AGAGT---TCTA------CAGT 40 + || ||.|| |||. |||| +FC12044_91407 1 -------------------------TCAAGGGTGGATCTTGGCTCCCAGT 25 + +Illumina_Smal 41 CCGA 44 + +FC12044_91407 26 ---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_76_246 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 15/38 (39.5%) +# Similarity: 15/38 (39.5%) +# Gaps: 19/38 (50.0%) +# Score: 60.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTC-AGAGT---TCTA--CAGTCCGACGATC 32 + || ||.|| |||. | ||| |..| +FC12044_91407 1 --------TCAAGGGTGGATCTTGGC--TCC--CAGT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 18/38 (47.4%) +# Score: 63.5 +# +# +#======================================= + +Illumina_Geno 1 GAT--CGGAAGAGCTCGTATGCCGTCTT---CTGCTTG 33 + | ||..|||| | |.||| |||| | +FC12044_91407 1 --TTGCGACAGAG-T--TTTGC--TCTTGTCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 59 +# Identity: 20/59 (33.9%) +# Similarity: 20/59 (33.9%) +# Gaps: 35/59 (59.3%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTT-TCCCTACACGACGCTC 49 + |. |||| || |||.|.| .||||| ||| +FC12044_91407 1 -----TT--GCGA-CA--GAGTTTT--GCTCTTGTCC------------- 25 + +Illumina_Geno 50 TTCCGATCT 58 + +FC12044_91407 26 --------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 23/41 (56.1%) +# Score: 69.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGA-----GCTCT--TCCGATCT 34 + ..| ||.|| || ||||| ||| +FC12044_91407 1 -------TTG-CGACA---GAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 15/35 (42.9%) +# Similarity: 15/35 (42.9%) +# Gaps: 13/35 (37.1%) +# Score: 43.0 +# +# +#======================================= + +Illumina_Pair 1 GAT--CGGAAGAGCGGTTCAGCAGGAATG-CCGAG 32 + | ||..||| |||..||. ..|| || +FC12044_91407 1 --TTGCGACAGA---GTTTTGCT--CTTGTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 59 +# Identity: 20/59 (33.9%) +# Similarity: 20/59 (33.9%) +# Gaps: 35/59 (59.3%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTT-TCCCTACACGACGCTC 49 + |. |||| || |||.|.| .||||| ||| +FC12044_91407 1 -----TT--GCGA-CA--GAGTTTT--GCTCTTGTCC------------- 25 + +Illumina_Pair 50 TTCCGATCT 58 + +FC12044_91407 26 --------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 63 +# Identity: 19/63 (30.2%) +# Similarity: 19/63 (30.2%) +# Gaps: 40/63 (63.5%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + ..| ||.|| |||.| || || +FC12044_91407 1 -------TTG-CGACA---GAGTT-----------TT-----------GC 17 + +Illumina_Pair 51 TCT--TCCGATCT 61 + ||| ||| +FC12044_91407 18 TCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 74.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGAC--------GCTCT--TCCGATCT 33 + || .|||| ||||| ||| +FC12044_91407 1 -------TT------GCGACAGAGTTTTGCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 15/46 (32.6%) +# Similarity: 15/46 (32.6%) +# Gaps: 30/46 (65.2%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCT--CGGCA-----TTCCTGCTGAACCGCTCT--TCCGATCT 37 + | ||.|| || ||||| ||| +FC12044_91407 1 -----TTGCGACAGAGTTTT-----------GCTCTTGTCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 7/29 (24.1%) +# Score: 60.0 +# +# +#======================================= + +Illumina_DpnI 1 GAT--CGTCGGACTGTAGAACTCTGAAC- 26 + | ||.|.||.|.|.| ||||...| +FC12044_91407 1 --TTGCGACAGAGTTTTG--CTCTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 15/33 (45.5%) +# Similarity: 15/33 (45.5%) +# Gaps: 15/33 (45.5%) +# Score: 78.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTT----CAGAGTTCTAC---AGTCCGAC 26 + || |||||||.|.| .|||| +FC12044_91407 1 -----TTGCGACAGAGTTTTGCTCTTGTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_DpnI 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 56.5 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGC--C---GTCTT-CTGCTTG--- 21 + .||| | ||.|| || |||| +FC12044_91407 1 ----TTGCGACAGAGTTTTGCT-CTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_DpnI 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 83.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTAC---AGTCCGA 44 + |. |||| |||||||.|.| .|||| +FC12044_91407 1 -----TT--GCGA-------------CAGAGTTTTGCTCTTGTCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 17/39 (43.6%) +# Similarity: 17/39 (43.6%) +# Gaps: 21/39 (53.8%) +# Score: 86.5 +# +# +#======================================= + +Illumina_DpnI 1 ---CGACAG-GTT---CAGAGTTCTACAGTCCGACGATC 32 + |||||| ||| | ||| .|||| +FC12044_91407 1 TTGCGACAGAGTTTTGC-----TCT--TGTCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 28 +# Identity: 13/28 (46.4%) +# Similarity: 13/28 (46.4%) +# Gaps: 10/28 (35.7%) +# Score: 49.0 +# +# +#======================================= + +Illumina_NlaI 1 T--CGGACTG----TAGAACTCTGAAC- 21 + | | |||.| |.| ||||...| +FC12044_91407 1 TTGC-GACAGAGTTTTG--CTCTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 15/36 (41.7%) +# Similarity: 15/36 (41.7%) +# Gaps: 18/36 (50.0%) +# Score: 78.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTT----CAGAGTTCTAC---AGTCCGACATG 29 + || |||||||.|.| .|||| +FC12044_91407 1 -----TTGCGACAGAGTTTTGCTCTTGTCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 9/36 (25.0%) +# Similarity: 9/36 (25.0%) +# Gaps: 24/36 (66.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CAAGCAGA----------AGACGGCATACGANN 23 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 14/30 (46.7%) +# Score: 56.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGC--C---GTCTT-CTGCTTG--- 21 + .||| | ||.|| || |||| +FC12044_91407 1 ----TTGCGACAGAGTTTTGCT-CTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 83.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTAC---AGTCCGA 44 + |. |||| |||||||.|.| .|||| +FC12044_91407 1 -----TT--GCGA-------------CAGAGTTTTGCTCTTGTCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 21/39 (53.8%) +# Score: 78.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTT----CAGAGTTCTAC---AGTCCGACATG 32 + || |||||||.|.| .|||| +FC12044_91407 1 --------TTGCGACAGAGTTTTGCTCTTGTCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_Smal 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 15/33 (45.5%) +# Similarity: 15/33 (45.5%) +# Gaps: 15/33 (45.5%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Smal 1 GTT----CAGAGTTCTAC---AGTCCGACGATC 26 + || |||||||.|.| .|||| +FC12044_91407 1 -TTGCGACAGAGTTTTGCTCTTGTCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 15/30 (50.0%) +# Similarity: 15/30 (50.0%) +# Gaps: 13/30 (43.3%) +# Score: 63.5 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGC--C---GTCTT-CTGCTTGT-- 22 + .||| | ||.|| || ||||| +FC12044_91407 1 ----TTGCGACAGAGTTTTGCT-CTTGTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 22/34 (64.7%) +# Score: 31.0 +# +# +#======================================= + +Illumina_Smal 1 ---CAAGCAGA----------AGACGGCATACGA 21 + |.| |||| .|.| | +FC12044_91407 1 TTGCGA-CAGAGTTTTGCTCTTGTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 18/47 (38.3%) +# Similarity: 18/47 (38.3%) +# Gaps: 25/47 (53.2%) +# Score: 83.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTAC---AGTCCGA 44 + |. |||| |||||||.|.| .|||| +FC12044_91407 1 -----TT--GCGA-------------CAGAGTTTTGCTCTTGTCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_303_427 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 17/39 (43.6%) +# Similarity: 17/39 (43.6%) +# Gaps: 21/39 (53.8%) +# Score: 86.5 +# +# +#======================================= + +Illumina_Smal 1 ---CGACAG-GTT---CAGAGTTCTACAGTCCGACGATC 32 + |||||| ||| | ||| .|||| +FC12044_91407 1 TTGCGACAGAGTTTTGC-----TCT--TGTCC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 22/40 (55.0%) +# Score: 55.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAG----AGCTC---GTATGCCGTCTTCTGCTTG 33 + || .| ||||| | |.||||.| +FC12044_91407 1 --TC---TGCTCCAGCTCCAAG-ACGCCGCC--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 19/60 (31.7%) +# Similarity: 19/60 (31.7%) +# Gaps: 37/60 (61.7%) +# Score: 99.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCC--CTACACGACGCT 48 + |||.| ||| ||.||.||||| +FC12044_91407 1 ----------------------TCTGC------TCCAGCTCCAAGACGC- 21 + +Illumina_Geno 49 CTTCCGATCT 58 + | .|| +FC12044_91407 22 C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 15/44 (34.1%) +# Similarity: 15/44 (34.1%) +# Gaps: 29/44 (65.9%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 ------CAAGCAG----AAGACGGCATACGAGCTCTTCCGATCT 34 + | ||| ||||| || || || +FC12044_91407 1 TCTGCTC---CAGCTCCAAGAC-GC---CG-------CC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 57.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG-------- 32 + || .|| |.||||. ||.|| +FC12044_91407 1 --TC-----TGC--TCCAGCT------CCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 60 +# Identity: 19/60 (31.7%) +# Similarity: 19/60 (31.7%) +# Gaps: 37/60 (61.7%) +# Score: 99.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCC--CTACACGACGCT 48 + |||.| ||| ||.||.||||| +FC12044_91407 1 ----------------------TCTGC------TCCAGCTCCAAGACGC- 21 + +Illumina_Pair 49 CTTCCGATCT 58 + | .|| +FC12044_91407 22 C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 66 +# Identity: 18/66 (27.3%) +# Similarity: 18/66 (27.3%) +# Gaps: 46/66 (69.7%) +# Score: 92.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCT----GA- 45 + ||| || |||.||| || +FC12044_91407 1 ---------------------------TCT--GC--TCCAGCTCCAAGAC 19 + +Illumina_Pair 46 ACCGCTCTTCCGATCT 61 + .|||| | +FC12044_91407 20 GCCGC-C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 87.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTT-TCC--CTACACGACGCTCTTCCGATCT 33 + |||. ||| ||.||.||||| | .|| +FC12044_91407 1 ----TCTGCTCCAGCTCCAAGACGC-C-GCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 18/42 (42.9%) +# Similarity: 18/42 (42.9%) +# Gaps: 22/42 (52.4%) +# Score: 92.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCT----GA-ACCGCTCTTCCGATCT 37 + ||| || |||.||| || .|||| | +FC12044_91407 1 ---TCT--GC--TCCAGCTCCAAGACGCCGC-C--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 19/35 (54.3%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 GATC-G-TCGGACTGTAGAACTCTGA-AC------ 26 + || | || |.| |||..| || +FC12044_91407 1 --TCTGCTC---CAG-----CTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 14/35 (40.0%) +# Similarity: 14/35 (40.0%) +# Gaps: 19/35 (54.3%) +# Score: 72.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAG-TCC--GAC------ 26 + ||.| ||.||| ||| ||| +FC12044_91407 1 ------TCTG----CTCCAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 51.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTCTTCTGCT---TG-------- 21 + || ||| || |.||| .| +FC12044_91407 1 TC---TGC--TC--CAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 12/54 (22.2%) +# Similarity: 12/54 (22.2%) +# Gaps: 39/54 (72.2%) +# Score: 64.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA----- 44 + ||.| ||.||| |||.| +FC12044_91407 1 -------------------------TCTG----CTCCAGCTCCAAGACGC 21 + +Illumina_DpnI 45 ---- 44 + +FC12044_91407 22 CGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 77.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAG-TCC--GACGATC---- 32 + ||.| ||.||| ||| |||| | +FC12044_91407 1 --------TCTG----CTCCAGCTCCAAGACG--CCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTG-T--AGAACTCTGA-AC------ 21 + .||| | || |||..| || +FC12044_91407 1 ----TCTGCTCCAG--CTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 15/37 (40.5%) +# Similarity: 15/37 (40.5%) +# Gaps: 20/37 (54.1%) +# Score: 67.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAG-TCC--GACATG----- 29 + ||.| ||.||| ||| ||| | +FC12044_91407 1 ------TCTG----CTCCAGCTCCAAGAC--GCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 22/35 (62.9%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAG----AAGACG--GCATACGANN 23 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 18/32 (56.2%) +# Score: 51.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTCTTCTGCT---TG-------- 21 + || ||| || |.||| .| +FC12044_91407 1 TC---TGC--TC--CAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 12/54 (22.2%) +# Similarity: 12/54 (22.2%) +# Gaps: 39/54 (72.2%) +# Score: 64.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA----- 44 + ||.| ||.||| |||.| +FC12044_91407 1 -------------------------TCTG----CTCCAGCTCCAAGACGC 21 + +Illumina_NlaI 45 ---- 44 + +FC12044_91407 22 CGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 15/40 (37.5%) +# Similarity: 15/40 (37.5%) +# Gaps: 23/40 (57.5%) +# Score: 67.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAG-TCC--GACATG----- 32 + ||.| ||.||| ||| ||| | +FC12044_91407 1 ---------TCTG----CTCCAGCTCCAAGAC--GCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 16/33 (48.5%) +# Similarity: 16/33 (48.5%) +# Gaps: 15/33 (45.5%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAG-TCC--GACGATC---- 26 + ||.| ||.||| ||| |||| | +FC12044_91407 1 --TCTG----CTCCAGCTCCAAGACG--CCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 12/32 (37.5%) +# Similarity: 12/32 (37.5%) +# Gaps: 17/32 (53.1%) +# Score: 50.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCTTCTGCT---TGT------- 22 + || ||| || |.||| .|. +FC12044_91407 1 TC---TGC--TC--CAGCTCCAAGACGCCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 13/33 (39.4%) +# Similarity: 13/33 (39.4%) +# Gaps: 20/33 (60.6%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 ------CAAGCAG----AAGACG--GCATACGA 21 + | ||| |||||| || | +FC12044_91407 1 TCTGCTC---CAGCTCCAAGACGCCGC---C-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 12/54 (22.2%) +# Similarity: 12/54 (22.2%) +# Gaps: 39/54 (72.2%) +# Score: 64.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAG-TCCGA----- 44 + ||.| ||.||| |||.| +FC12044_91407 1 -------------------------TCTG----CTCCAGCTCCAAGACGC 21 + +Illumina_Smal 45 ---- 44 + +FC12044_91407 22 CGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_31_299 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 21/39 (53.8%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAG-TCC--GACGATC---- 32 + ||.| ||.||| ||| |||| | +FC12044_91407 1 --------TCTG----CTCCAGCTCCAAGACG--CCGCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 12/37 (32.4%) +# Similarity: 12/37 (32.4%) +# Gaps: 16/37 (43.2%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAA----GAGCTCGTATGCCGTCTTCTGCTTG 33 + ..||||. |.||..|.|.|.|| +FC12044_91407 1 -TACGGAGCCGCGGGCGGGAAAGGCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 69.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCAC---CGAGATCTACACTCTTTCCCTACACGACGC 47 + |||||.| ||.| ||.|| |.|.|| +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGGA------------------AAGGCG- 25 + +Illumina_Geno 48 TCTTCCGATCT 58 + +FC12044_91407 26 ----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 9/46 (19.6%) +# Similarity: 9/46 (19.6%) +# Gaps: 33/46 (71.7%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Geno 1 ----------CAAGCAG--AAGACGGCATACGAGCTCTTCCGATCT 34 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG--------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 17/32 (53.1%) +# Similarity: 17/32 (53.1%) +# Gaps: 7/32 (21.9%) +# Score: 75.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG 32 + ..||||...|||| ||.||||.|.|| +FC12044_91407 1 -TACGGAGCCGCGG----GCGGGAAAGGCG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 17/61 (27.9%) +# Similarity: 17/61 (27.9%) +# Gaps: 39/61 (63.9%) +# Score: 69.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCAC---CGAGATCTACACTCTTTCCCTACACGACGC 47 + |||||.| ||.| ||.|| |.|.|| +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGGA------------------AAGGCG- 25 + +Illumina_Pair 48 TCTTCCGATCT 58 + +FC12044_91407 26 ----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 17/64 (26.6%) +# Similarity: 17/64 (26.6%) +# Gaps: 42/64 (65.6%) +# Score: 56.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAAC--- 47 + |||| ||.| |.||| ||.|.|. +FC12044_91407 1 ----------------TACG-GAGC---CGCGG-------GCGGGAAAGG 23 + +Illumina_Pair 48 CGCTCTTCCGATCT 61 + || +FC12044_91407 24 CG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 29.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGACGCTCTTC-CGATCT--------- 33 + |||. || || | ||..|. +FC12044_91407 1 ------------TACG-GA-GC----CGCGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 11/48 (22.9%) +# Similarity: 11/48 (22.9%) +# Gaps: 34/48 (70.8%) +# Score: 45.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGCTCTT---C--------CGATCT 37 + |.|.||.|||| | || +FC12044_91407 1 ---------------TACGGAGCCGC----GGGCGGGAAAGGCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 13/35 (37.1%) +# Similarity: 13/35 (37.1%) +# Gaps: 19/35 (54.3%) +# Score: 35.0 +# +# +#======================================= + +Illumina_DpnI 1 ----GAT-CGTCGGACTGTAGAACTCTGAA---C- 26 + ||. || |||.|.| ||| | +FC12044_91407 1 TACGGAGCCG-CGGGCGG---------GAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 9/40 (22.5%) +# Similarity: 9/40 (22.5%) +# Gaps: 29/40 (72.5%) +# Score: 44.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGTCCGAC-------------- 26 + |.|.||| ||| | +FC12044_91407 1 -----TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 31.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATGCCGTCTTC-TGCTTG--------- 21 + .||.| |||| | .|| | +FC12044_91407 1 TACGGA-GCCG----CGGGC--GGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 19/44 (43.2%) +# Similarity: 19/44 (43.2%) +# Gaps: 19/44 (43.2%) +# Score: 71.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + |||||.| ||.|.|.|.|| |.|| | || +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGG---AAAG-------G--CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 11/42 (26.2%) +# Similarity: 11/42 (26.2%) +# Gaps: 27/42 (64.3%) +# Score: 52.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGTCCGACGATC---------- 32 + |.|.||| ||| ||..| +FC12044_91407 1 -------TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGGA-CTGTAGAACTCTGAAC---------- 21 + .|||| | |.| |..| +FC12044_91407 1 TACGGAGC-------CGC-GGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 10/40 (25.0%) +# Similarity: 10/40 (25.0%) +# Gaps: 26/40 (65.0%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCCGACATG----------- 29 + |.|.||| ||| |..| +FC12044_91407 1 -----TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 22/35 (62.9%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAG--AAGACGGCATACGANN 23 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATGCCGTCTTC-TGCTTG--------- 21 + .||.| |||| | .|| | +FC12044_91407 1 TACGGA-GCCG----CGGGC--GGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 19/44 (43.2%) +# Similarity: 19/44 (43.2%) +# Gaps: 19/44 (43.2%) +# Score: 71.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + |||||.| ||.|.|.|.|| |.|| | || +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGG---AAAG-------G--CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 10/43 (23.3%) +# Similarity: 10/43 (23.3%) +# Gaps: 29/43 (67.4%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTCAGAGTTCTACAGTCCGACATG----------- 32 + |.|.||| ||| |..| +FC12044_91407 1 --------TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 11/36 (30.6%) +# Similarity: 11/36 (30.6%) +# Gaps: 21/36 (58.3%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGTCCGACGATC---------- 26 + |.|.||| ||| ||..| +FC12044_91407 1 -TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 11/30 (36.7%) +# Similarity: 11/30 (36.7%) +# Gaps: 13/30 (43.3%) +# Score: 28.5 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATGCCGTCTTC-TGCTTGT------ 22 + .||.| |||| | .||..|. +FC12044_91407 1 TACGGA-GCCG----CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 9/33 (27.3%) +# Similarity: 9/33 (27.3%) +# Gaps: 20/33 (60.6%) +# Score: 47.5 +# +# +#======================================= + +Illumina_Smal 1 ----------CAAGCAG--AAGACGGCATACGA 21 + |..||.| |||.|| +FC12044_91407 1 TACGGAGCCGCGGGCGGGAAAGGCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 19/44 (43.2%) +# Similarity: 19/44 (43.2%) +# Gaps: 19/44 (43.2%) +# Score: 71.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGA 44 + |||||.| ||.|.|.|.|| |.|| | || +FC12044_91407 1 -----TACGGAG-CCGCGGGCGGG---AAAG-------G--CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_553_135 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 11/42 (26.2%) +# Similarity: 11/42 (26.2%) +# Gaps: 27/42 (64.3%) +# Score: 52.0 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGTCCGACGATC---------- 32 + |.|.||| ||| ||..| +FC12044_91407 1 -------TACGGAG---------CCG-CGGGCGGGAAAGGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 14/43 (32.6%) +# Similarity: 14/43 (32.6%) +# Gaps: 28/43 (65.1%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Geno 1 --------GATCGGAAGAGCTCG--TATGCCGTCTTCTGCTTG 33 + |.|| ||| || ||| || | +FC12044_91407 1 CCTCCCAGGTTC--AAG----CGATTAT-CC-T---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 18/64 (28.1%) +# Similarity: 18/64 (28.1%) +# Gaps: 45/64 (70.3%) +# Score: 73.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TACA--CGAC 45 + || |||| |.|| ||| +FC12044_91407 1 ----------------CC---------------TCCCAGGTTCAAGCGA- 18 + +Illumina_Geno 46 GCTCTTCCGAT-CT 58 + || || || +FC12044_91407 19 ----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 13/46 (28.3%) +# Similarity: 13/46 (28.3%) +# Gaps: 33/46 (71.7%) +# Score: 61.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTC-----TTC---CGAT----CT 34 + | ||| ||| |||| || +FC12044_91407 1 ------------------C---CTCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 14/40 (35.0%) +# Similarity: 14/40 (35.0%) +# Gaps: 23/40 (57.5%) +# Score: 55.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGC------GGTTC-AGCAGGAATG-CCGAG 32 + | ||||| ||| ||.|. ||. +FC12044_91407 1 -----------CCTCCCAGGTTCAAGC--GATTATCCT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 18/64 (28.1%) +# Similarity: 18/64 (28.1%) +# Gaps: 45/64 (70.3%) +# Score: 73.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCC---TACA--CGAC 45 + || |||| |.|| ||| +FC12044_91407 1 ----------------CC---------------TCCCAGGTTCAAGCGA- 18 + +Illumina_Pair 46 GCTCTTCCGAT-CT 58 + || || || +FC12044_91407 19 ----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 71 +# Identity: 15/71 (21.1%) +# Similarity: 15/71 (21.1%) +# Gaps: 56/71 (78.9%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + ||| || +FC12044_91407 1 -------------------------------------CCT------CC-- 5 + +Illumina_Pair 51 TC---TTC---CGAT----CT 61 + | ||| |||| || +FC12044_91407 6 -CAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 18/39 (46.2%) +# Similarity: 18/39 (46.2%) +# Gaps: 20/39 (51.3%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCC---TACA--CGACGCTCTTCCGAT-CT 33 + | | |||| |.|| ||| || || || +FC12044_91407 1 ---C-C--TCCCAGGTTCAAGCGA-----TT---ATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 15/47 (31.9%) +# Similarity: 15/47 (31.9%) +# Gaps: 32/47 (68.1%) +# Score: 70.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGCTC---TTC---CGAT----CT 37 + ||| || | ||| |||| || +FC12044_91407 1 -------------CCT------CC---CAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 7/42 (16.7%) +# Similarity: 7/42 (16.7%) +# Gaps: 33/42 (78.6%) +# Score: 32.0 +# +# +#======================================= + +Illumina_DpnI 1 ----------------GATCGTCGGACTGTAGAACTCTGAAC 26 + |||..|| || +FC12044_91407 1 CCTCCCAGGTTCAAGCGATTATC---CT-------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 16/31 (51.6%) +# Similarity: 16/31 (51.6%) +# Gaps: 11/31 (35.5%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 ----ACAGGTTC-AGAGTTCTACAGTCCGAC 26 + .||||||| ||.|.| || |||. +FC12044_91407 1 CCTCCCAGGTTCAAGCGAT-TA---TCCT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 20/33 (60.6%) +# Score: 45.5 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTC-----TTC-TGC--TTG---- 21 + || || ||| .|| ||. +FC12044_91407 1 -------CC-TCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 101.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTTCTACAGTCCGA 44 + ||.|| ||||||| ||.|.| || |||. +FC12044_91407 1 -------------CCTCC--CAGGTTCAAGCGAT-TA---TCCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 19/38 (50.0%) +# Score: 76.0 +# +# +#======================================= + +Illumina_DpnI 1 ----CGACAGGTTCAGAGTTCTACAGTCCGACGATC-- 32 + | |||||||| || |||..||| +FC12044_91407 1 CCTCC--CAGGTTCA-AG----------CGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 8/31 (25.8%) +# Similarity: 8/31 (25.8%) +# Gaps: 16/31 (51.6%) +# Score: 31.0 +# +# +#======================================= + +Illumina_NlaI 1 ----------TCGGACTGTAGAACTCTGAAC 21 + ||...| |...|.| || +FC12044_91407 1 CCTCCCAGGTTCAAGC-GATTATC-CT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 14/34 (41.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_NlaI 1 ----ACAGGTTC-AGAGTTCTACAGTCCGACATG 29 + .||||||| ||.|.| || ||| | +FC12044_91407 1 CCTCCCAGGTTCAAGCGAT-TA---TCC----T- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 7/34 (20.6%) +# Similarity: 7/34 (20.6%) +# Gaps: 20/34 (58.8%) +# Score: 37.5 +# +# +#======================================= + +Illumina_NlaI 1 -----------CAAGCAGAAGACGGCATACGANN 23 + |||||......| |. +FC12044_91407 1 CCTCCCAGGTTCAAGCGATTATC--CT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 20/33 (60.6%) +# Score: 45.5 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTC-----TTC-TGC--TTG---- 21 + || || ||| .|| ||. +FC12044_91407 1 -------CC-TCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 101.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTTCTACAGTCCGA 44 + ||.|| ||||||| ||.|.| || |||. +FC12044_91407 1 -------------CCTCC--CAGGTTCAAGCGAT-TA---TCCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 19/36 (52.8%) +# Similarity: 19/36 (52.8%) +# Gaps: 15/36 (41.7%) +# Score: 84.0 +# +# +#======================================= + +Illumina_NlaI 1 ---CCGACAGGTTC-AGAGTTCTACAGTCCGACATG 32 + || ||||||| ||.|.| || ||| | +FC12044_91407 1 CCTCC--CAGGTTCAAGCGAT-TA---TCC----T- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 54.5 +# +# +#======================================= + +Illumina_Smal 1 --------GTTCAGAGTTCTACAGTCCGACGATC-- 26 + ||||| || |||..||| +FC12044_91407 1 CCTCCCAGGTTCA-AG----------CGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 19/33 (57.6%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTC-----TTC-TGC--TTGT--- 22 + || || ||| .|| ||.| +FC12044_91407 1 -------CC-TCCCAGGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_Smal 1 ----CAAGCAGAAGACGG--CATACGA------- 21 + | || || ||..||| +FC12044_91407 1 CCTCC---CA------GGTTCAAGCGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 101.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTTCTACAGTCCGA 44 + ||.|| ||||||| ||.|.| || |||. +FC12044_91407 1 -------------CCTCC--CAGGTTCAAGCGAT-TA---TCCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_139_74 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 17/38 (44.7%) +# Similarity: 17/38 (44.7%) +# Gaps: 19/38 (50.0%) +# Score: 76.0 +# +# +#======================================= + +Illumina_Smal 1 ----CGACAGGTTCAGAGTTCTACAGTCCGACGATC-- 32 + | |||||||| || |||..||| +FC12044_91407 1 CCTCC--CAGGTTCA-AG----------CGATTATCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 12/43 (27.9%) +# Similarity: 12/43 (27.9%) +# Gaps: 28/43 (65.1%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGT-ATGCCGTCTTCTGCTTG--------- 33 + || |||.||.| |.|| | +FC12044_91407 1 --------------GTCATGGCGGC--CCGC--GCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Geno 1 --AATGATACGGCGACCAC-CGAGATCTACACTCTTTCCCTACACGA-CG 46 + .||| .|||| ||.| ||.| || || +FC12044_91407 1 GTCATG--GCGGC--CCGCGCGGG---------------------GAGCG 25 + +Illumina_Geno 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 29/44 (65.9%) +# Score: 46.0 +# +# +#======================================= + +Illumina_Geno 1 --CAAGCAGAAGACGGCATAC--------GAGCTCTTCCGATCT 34 + | |.|.|||| | ||||. +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 17/35 (48.6%) +# Similarity: 17/35 (48.6%) +# Gaps: 13/35 (37.1%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTC---AGCAGGAATGCCGAG 32 + .|| |.| |||| | .||.||.| | || +FC12044_91407 1 -GTC--ATG-GCGG--CCCGCGCGGGGA-G-CG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 41/62 (66.1%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Pair 1 --AATGATACGGCGACCAC-CGAGATCTACACTCTTTCCCTACACGA-CG 46 + .||| .|||| ||.| ||.| || || +FC12044_91407 1 GTCATG--GCGGC--CCGCGCGGG---------------------GAGCG 25 + +Illumina_Pair 47 CTCTTCCGATCT 58 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 70 +# Identity: 14/70 (20.0%) +# Similarity: 14/70 (20.0%) +# Gaps: 54/70 (77.1%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCT---CGGCATTCCTGCTGAAC 47 + |||. |||| | +FC12044_91407 1 --------------------------GTCATGGCGGC------------C 12 + +Illumina_Pair 48 CGCTCTTC------CGATCT 61 + ||| .| || +FC12044_91407 13 CGC---GCGGGGAGCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 28/43 (65.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Pair 1 -ACACTCTTT-----CCCTACACG----ACGCTCTTCCGATCT 33 + .|| | ||| .|.|| .|| +FC12044_91407 1 GTCA-----TGGCGGCCC-GCGCGGGGAGCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 14/46 (30.4%) +# Similarity: 14/46 (30.4%) +# Gaps: 30/46 (65.2%) +# Score: 60.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCT---CGGCATTCCTGCTGAACCGCTCTTC------CGATCT 37 + |||. |||| |||| .| || +FC12044_91407 1 --GTCATGGCGGC------------CCGC---GCGGGGAGCG---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 15/33 (45.5%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGT--CGGACTGTAGAACTC----TGAAC- 26 + .||.| |||.| |.| .||.| +FC12044_91407 1 -GTCATGGCGGCC-------CGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 11/36 (30.6%) +# Similarity: 11/36 (30.6%) +# Gaps: 21/36 (58.3%) +# Score: 37.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTACAGTCCGAC---------- 26 + | |||..| |.|.||| | +FC12044_91407 1 ---G--TCATGG-----CGGCCCG-CGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_DpnI 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATGCCGTCTTCTGCTTG--------- 21 + || |||.||.| |.|| | +FC12044_91407 1 GTC--ATGGCGGC--CCGC--GCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_DpnI 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCAC-CGACAGGTTCAGAGTTCTACAGTCCGA 44 + .|.|| ||||.|| | || |.|| ||| || +FC12044_91407 1 -GTCAT--GGCGGCC-CGCG-CGGG----GAG----------CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 45.0 +# +# +#======================================= + +Illumina_DpnI 1 CGACA-GGTTCAGAGTTCTACAGTCCGACGATC-------- 32 + |.|| || |.|.||| || | +FC12044_91407 1 -GTCATGG------------CGGCCCG-CG--CGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 26.0 +# +# +#======================================= + +Illumina_NlaI 1 ----T--CGGACTGTAGAACTC----TGAAC- 21 + | |||.| |.| .||.| +FC12044_91407 1 GTCATGGCGGCC-------CGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 5/48 (10.4%) +# Similarity: 5/48 (10.4%) +# Gaps: 42/48 (87.5%) +# Score: 34.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCCGACATG------------------- 29 + |.|||| +FC12044_91407 1 -----------------------GTCATGGCGGCCCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 9/34 (26.5%) +# Similarity: 9/34 (26.5%) +# Gaps: 20/34 (58.8%) +# Score: 35.5 +# +# +#======================================= + +Illumina_NlaI 1 --CAAGCAGAAGACGGC---------ATACGANN 23 + | |.|.|||| ...|| +FC12044_91407 1 GTC------ATGGCGGCCCGCGCGGGGAGCG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 16/31 (51.6%) +# Score: 39.5 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATGCCGTCTTCTGCTTG--------- 21 + || |||.||.| |.|| | +FC12044_91407 1 GTC--ATGGCGGC--CCGC--GCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_NlaI 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCAC-CGACAGGTTCAGAGTTCTACAGTCCGA 44 + .|.|| ||||.|| | || |.|| ||| || +FC12044_91407 1 -GTCAT--GGCGGCC-CGCG-CGGG----GAG----------CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 31/44 (70.5%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 -----------CCG-ACAGGTTCAGAGTTCTACAGTCCGACATG 32 + ||| .|.|| ||| || +FC12044_91407 1 GTCATGGCGGCCCGCGCGGG----GAG----------CG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Smal 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 13/34 (38.2%) +# Similarity: 13/34 (38.2%) +# Gaps: 17/34 (50.0%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTACAGTCCGACGATC-------- 26 + | |||..| |.|.||| || | +FC12044_91407 1 G-TCATGG-----CGGCCCG-CG--CGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 12/34 (35.3%) +# Similarity: 12/34 (35.3%) +# Gaps: 21/34 (61.8%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATG-----CCGTCTTC------TGCTTGT 22 + || ||| ||| | .|| | +FC12044_91407 1 GTC--ATGGCGGCCCG----CGCGGGGAGC--G- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 36.0 +# +# +#======================================= + +Illumina_Smal 1 --CAAGCAGAAGACGGCATAC--------GA--- 21 + | |.|.|||| | || +FC12044_91407 1 GTC------ATGGCGGC---CCGCGCGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 20/45 (44.4%) +# Similarity: 20/45 (44.4%) +# Gaps: 21/45 (46.7%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCAC-CGACAGGTTCAGAGTTCTACAGTCCGA 44 + .|.|| ||||.|| | || |.|| ||| || +FC12044_91407 1 -GTCAT--GGCGGCC-CGCG-CGGG----GAG----------CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_108_33 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 45.0 +# +# +#======================================= + +Illumina_Smal 1 CGACA-GGTTCAGAGTTCTACAGTCCGACGATC-------- 32 + |.|| || |.|.||| || | +FC12044_91407 1 -GTCATGG------------CGGCCCG-CG--CGGGGAGCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 30/44 (68.2%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Geno 1 -------GATC----GGAAGAGCTCGTATGCCGTCTTCTGCTTG 33 + |.|| .|||||| ||| +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAG-TCG------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Geno 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 37.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGA-GATCTACACTCTTTCCCTACACGA----- 44 + ||.|. |.| ||||. |.|| +FC12044_91407 1 ---------------ACAGTGGGT------TCTTA------AAGAAGAGT 23 + +Illumina_Geno 45 CGCTCTTCCGATCT 58 + || +FC12044_91407 24 CG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 11/48 (22.9%) +# Similarity: 11/48 (22.9%) +# Gaps: 37/48 (77.1%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Geno 1 ----------C--AAGCAGAAGA--CGGCATACGAGCTCTTCCGATCT 34 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG--------------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Geno 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 17/36 (47.2%) +# Similarity: 17/36 (47.2%) +# Gaps: 15/36 (41.7%) +# Score: 58.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTC----AGCAGGAATGCCGAG 32 + .| ||.| ||||| || |.||.| || +FC12044_91407 1 --AC---AGTG-GGTTCTTAAAG-AAGAGT--CG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Pair 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 64 +# Identity: 14/64 (21.9%) +# Similarity: 14/64 (21.9%) +# Gaps: 45/64 (70.3%) +# Score: 37.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGA-GATCTACACTCTTTCCCTACACGA----- 44 + ||.|. |.| ||||. |.|| +FC12044_91407 1 ---------------ACAGTGGGT------TCTTA------AAGAAGAGT 23 + +Illumina_Pair 45 CGCTCTTCCGATCT 58 + || +FC12044_91407 24 CG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 14/68 (20.6%) +# Similarity: 14/68 (20.6%) +# Gaps: 50/68 (73.5%) +# Score: 48.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGG---CATACGAGA----TCGGTCTCGGCATTCCTGCT 43 + .|||. || |.|| .||| ||| +FC12044_91407 1 ---ACAGT----GGGTTCTTA-AAGAAGAGTCG----------------- 25 + +Illumina_Pair 44 GAACCGCTCTTCCGATCT 61 + +FC12044_91407 26 ------------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 11/45 (24.4%) +# Similarity: 11/45 (24.4%) +# Gaps: 32/45 (71.1%) +# Score: 36.5 +# +# +#======================================= + +Illumina_Pair 1 ACAC-------TCTTTCCCTACACGA-----CGCTCTTCCGATCT 33 + || ||||. |.|| || +FC12044_91407 1 --ACAGTGGGTTCTTA------AAGAAGAGTCG------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 16/39 (41.0%) +# Similarity: 16/39 (41.0%) +# Gaps: 16/39 (41.0%) +# Score: 40.5 +# +# +#======================================= + +Illumina_Pair 1 -CGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGA-TCT 37 + |.|| || .|||.|...||| || ||. +FC12044_91407 1 ACAGT---GG-GTTCTTAAAGAA----------GAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 12/35 (34.3%) +# Similarity: 12/35 (34.3%) +# Gaps: 19/35 (54.3%) +# Score: 48.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCT----GAA----C- 26 + ||.||.|.. ||| ||| | +FC12044_91407 1 ---------ACAGTGGGT-TCTTAAAGAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 15/36 (41.7%) +# Similarity: 15/36 (41.7%) +# Gaps: 21/36 (58.3%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 ACA---GGTTC-------AGAGTTCTACAGTCCGAC 26 + ||| ||||| ||||| || +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGT---------CG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 7/36 (19.4%) +# Similarity: 7/36 (19.4%) +# Gaps: 26/36 (72.2%) +# Score: 24.5 +# +# +#======================================= + +Illumina_DpnI 1 ---------TCGTA------TGCCGTCTTCTGCTTG 21 + ||.|| .|.|| +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGTCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 15/54 (27.8%) +# Similarity: 15/54 (27.8%) +# Gaps: 39/54 (72.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACA---GGTTC-------AGAGTTCTACAGT 40 + ||| ||||| ||||| +FC12044_91407 1 -------------------ACAGTGGGTTCTTAAAGAAGAGT-------- 23 + +Illumina_DpnI 41 CCGA 44 + || +FC12044_91407 24 -CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 11/34 (32.4%) +# Score: 67.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTCAGAGTTCTACAGTCCGACGA-TC- 32 + |||| .|.|||||..|. ||.|| || +FC12044_91407 1 --ACAG----TGGGTTCTTAAA---GAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 48.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACTGTAGAACTCT----GAA----C- 21 + ||.||.|.. ||| ||| | +FC12044_91407 1 ----ACAGTGGGT-TCTTAAAGAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 24/39 (61.5%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 ACA---GGTTC-------AGAGTTCTACAGTCCGACATG 29 + ||| ||||| ||||| || +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGT---------CG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 11/37 (29.7%) +# Similarity: 11/37 (29.7%) +# Gaps: 26/37 (70.3%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------C--AAGCAGAAGA--CGGCATACGANN 23 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 7/36 (19.4%) +# Similarity: 7/36 (19.4%) +# Gaps: 26/36 (72.2%) +# Score: 24.5 +# +# +#======================================= + +Illumina_NlaI 1 ---------TCGTA------TGCCGTCTTCTGCTTG 21 + ||.|| .|.|| +FC12044_91407 1 ACAGTGGGTTCTTAAAGAAGAGTCG----------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 15/54 (27.8%) +# Similarity: 15/54 (27.8%) +# Gaps: 39/54 (72.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACA---GGTTC-------AGAGTTCTACAGT 40 + ||| ||||| ||||| +FC12044_91407 1 -------------------ACAGTGGGTTCTTAAAGAAGAGT-------- 23 + +Illumina_NlaI 41 CCGA 44 + || +FC12044_91407 24 -CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 15/42 (35.7%) +# Similarity: 15/42 (35.7%) +# Gaps: 27/42 (64.3%) +# Score: 67.0 +# +# +#======================================= + +Illumina_NlaI 1 CCGACA---GGTTC-------AGAGTTCTACAGTCCGACATG 32 + ||| ||||| ||||| || +FC12044_91407 1 ---ACAGTGGGTTCTTAAAGAAGAGT---------CG----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 15/30 (50.0%) +# Similarity: 15/30 (50.0%) +# Gaps: 9/30 (30.0%) +# Score: 57.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGA--GTTCTACAGTCCGACGA-TC- 26 + .|||. |||||..|. ||.|| || +FC12044_91407 1 --ACAGTGGGTTCTTAAA---GAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 7/38 (18.4%) +# Similarity: 7/38 (18.4%) +# Gaps: 29/38 (76.3%) +# Score: 25.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCCGTCTTCTG------CTT--------GT-- 22 + .|.| ||| || +FC12044_91407 1 -------------ACAGTGGGTTCTTAAAGAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 11/35 (31.4%) +# Similarity: 11/35 (31.4%) +# Gaps: 24/35 (68.6%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 ----------C--AAGCAGAAGA--CGGCATACGA 21 + | || |||||| || +FC12044_91407 1 ACAGTGGGTTCTTAA--AGAAGAGTCG-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 54 +# Identity: 15/54 (27.8%) +# Similarity: 15/54 (27.8%) +# Gaps: 39/54 (72.2%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACA---GGTTC-------AGAGTTCTACAGT 40 + ||| ||||| ||||| +FC12044_91407 1 -------------------ACAGTGGGTTCTTAAAGAAGAGT-------- 23 + +Illumina_Smal 41 CCGA 44 + || +FC12044_91407 24 -CG- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_980_965 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 11/34 (32.4%) +# Score: 67.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTCAGAGTTCTACAGTCCGACGA-TC- 32 + |||| .|.|||||..|. ||.|| || +FC12044_91407 1 --ACAG----TGGGTTCTTAAA---GAAGAGTCG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 18/37 (48.6%) +# Similarity: 18/37 (48.6%) +# Gaps: 16/37 (43.2%) +# Score: 77.5 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTATGC-CGTCTT--CTGCTTG- 33 + |.|| ||.| || ||.||| | |||| +FC12044_91407 1 -AACG--AGGG-------GCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 19/62 (30.6%) +# Similarity: 19/62 (30.6%) +# Gaps: 41/62 (66.1%) +# Score: 65.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATAC----GGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + .|| |||| ||| ||| ||| +FC12044_91407 1 -----AACGAGGGGCG-----CGA----------CTT---------GAC- 20 + +Illumina_Geno 47 CTCTTCCGATCT 58 + ||| |. +FC12044_91407 21 --CTT--GG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 18/37 (48.6%) +# Similarity: 18/37 (48.6%) +# Gaps: 15/37 (40.5%) +# Score: 64.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT--- 34 + |.| ||.| ||| .||| ||| ||.|| +FC12044_91407 1 --AAC-GAGG--GGC--GCGA---CTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 15/33 (45.5%) +# Similarity: 15/33 (45.5%) +# Gaps: 9/33 (27.3%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG- 32 + |.| ||.|.||| |..|..|| ||..| +FC12044_91407 1 -AAC-GAGGGGCG---CGACTTGA---CCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 19/62 (30.6%) +# Similarity: 19/62 (30.6%) +# Gaps: 41/62 (66.1%) +# Score: 65.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATAC----GGCGACCACCGAGATCTACACTCTTTCCCTACACGACG 46 + .|| |||| ||| ||| ||| +FC12044_91407 1 -----AACGAGGGGCG-----CGA----------CTT---------GAC- 20 + +Illumina_Pair 47 CTCTTCCGATCT 58 + ||| |. +FC12044_91407 21 --CTT--GG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 62 +# Identity: 17/62 (27.4%) +# Similarity: 17/62 (27.4%) +# Gaps: 38/62 (61.3%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCAT-TCCTGCTGAACCG 49 + .||||| ||.|.||.|.| .||| ||. +FC12044_91407 1 ----------------AACGAG---GGGCGCGACTTGACCT--TGG---- 25 + +Illumina_Pair 50 CTCTTCCGATCT 61 + +FC12044_91407 26 ------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 15/41 (36.6%) +# Similarity: 15/41 (36.6%) +# Gaps: 24/41 (58.5%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACACGA----CGC-TCTTCCGATCT--- 33 + | |||| ||| .||| ||.|| +FC12044_91407 1 -------------A-ACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 13/47 (27.7%) +# Similarity: 13/47 (27.7%) +# Gaps: 32/47 (68.1%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAAC------CGC-TCTTCCGATCT--- 37 + ||| ||| .||| ||.|| +FC12044_91407 1 --------------------AACGAGGGGCGCGACTT--GACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 15/31 (48.4%) +# Similarity: 15/31 (48.4%) +# Gaps: 11/31 (35.5%) +# Score: 56.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGT----CG-GACTGTAGAACTCTGAAC 26 + |.||. || ||| |.||.|| ||. +FC12044_91407 1 -AACGAGGGGCGCGAC--TTGACCT-TGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 11/39 (28.2%) +# Similarity: 11/39 (28.2%) +# Gaps: 27/39 (69.2%) +# Score: 39.5 +# +# +#======================================= + +Illumina_DpnI 1 -AC-AGGTTCAGAGTTCTACAGTC-CGAC---------- 26 + || ||| |.| |||| +FC12044_91407 1 AACGAGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 60.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGT-ATGC-CGTCTT--CTGCTTG- 21 + .||. ..|| ||.||| | |||| +FC12044_91407 1 AACGAGGGGCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 59.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC----GGCGACCACCGACAGGTTCAGAGTTCTACAGTCC---G 43 + .|| |||| |||| || |.|| | +FC12044_91407 1 -----AACGAGGGGCG-----CGAC---TT------------GACCTTGG 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 14/41 (34.1%) +# Similarity: 14/41 (34.1%) +# Gaps: 25/41 (61.0%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 --CGACAGGTTCAGAGTTCTACAGTC-CGAC--GATC---- 32 + || ||| |.| |||| ||.| +FC12044_91407 1 AACG--AGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 12/30 (40.0%) +# Similarity: 12/30 (40.0%) +# Gaps: 14/30 (46.7%) +# Score: 43.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCG--------GACTGTAGAACTCTGAAC 21 + .|| ||| |.||.|| ||. +FC12044_91407 1 AACGAGGGGCGCGAC--TTGACCT-TGG-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 13/39 (33.3%) +# Similarity: 13/39 (33.3%) +# Gaps: 24/39 (61.5%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 -AC-AGGTTCAGAGTTCTACAGTC-CGACATG------- 29 + || ||| |.| ||||.|| +FC12044_91407 1 AACGAGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 14/31 (45.2%) +# Score: 37.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAG---ACGGCAT-AC----GANN 23 + |.| ||.| .||.|.| || | +FC12044_91407 1 --AAC-GAGGGGCGCGACTTGACCTTGG--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 8/27 (29.6%) +# Score: 60.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGT-ATGC-CGTCTT--CTGCTTG- 21 + .||. ..|| ||.||| | |||| +FC12044_91407 1 AACGAGGGGCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 59.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC----GGCGACCACCGACAGGTTCAGAGTTCTACAGTCC---G 43 + .|| |||| |||| || |.|| | +FC12044_91407 1 -----AACGAGGGGCG-----CGAC---TT------------GACCTTGG 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 13/41 (31.7%) +# Similarity: 13/41 (31.7%) +# Gaps: 25/41 (61.0%) +# Score: 49.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGAC-AGGTTCAGAGTTCTACAGTC-CGACATG------- 32 + .|| ||| |.| ||||.|| +FC12044_91407 1 --AACGAGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 11/36 (30.6%) +# Similarity: 11/36 (30.6%) +# Gaps: 21/36 (58.3%) +# Score: 37.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTAC---AGTC-CGAC--GATC---- 26 + .|| .|.| |||| ||.| +FC12044_91407 1 -----------AACGAGGGGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 27 +# Identity: 14/27 (51.9%) +# Similarity: 14/27 (51.9%) +# Gaps: 7/27 (25.9%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGT-ATGC-CGTCTT--CTGCTTGT 22 + .||. ..|| ||.||| | ||||. +FC12044_91407 1 AACGAGGGGCGCGACTTGAC--CTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 41.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA----------- 21 + |.| ||.| ||| .||| +FC12044_91407 1 --AAC-GAGG--GGC--GCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 16/51 (31.4%) +# Similarity: 16/51 (31.4%) +# Gaps: 33/51 (64.7%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC----GGCGACCACCGACAGGTTCAGAGTTCTACAGTCC---G 43 + .|| |||| |||| || |.|| | +FC12044_91407 1 -----AACGAGGGGCG-----CGAC---TT------------GACCTTGG 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_981_857 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 14/41 (34.1%) +# Similarity: 14/41 (34.1%) +# Gaps: 25/41 (61.0%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 --CGACAGGTTCAGAGTTCTACAGTC-CGAC--GATC---- 32 + || ||| |.| |||| ||.| +FC12044_91407 1 AACG--AGG--------------GGCGCGACTTGACCTTGG 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 11/44 (25.0%) +# Similarity: 11/44 (25.0%) +# Gaps: 30/44 (68.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCTCGTAT---GCCGTCTTCTG-----CTTG--- 33 + |.| .|| | |.| |||| +FC12044_91407 1 ---------------TTTCCCACC--C--CAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC---GACGC 47 + |||||| ||.| ||.|| +FC12044_91407 1 -------------------------------TTTCCC-ACCCCAGGAAGC 18 + +Illumina_Geno 48 TCTTCCGATCT 58 + ||| |..| +FC12044_91407 19 -CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 27/43 (62.8%) +# Score: 48.5 +# +# +#======================================= + +Illumina_Geno 1 ---------CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT 34 + | |||.| ||| ||| |..| +FC12044_91407 1 TTTCCCACCC---CAGGA-----------AGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 27/42 (64.3%) +# Score: 55.5 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTT-------CAGCAGGAATGCCGAG--- 32 + || | |||||| |||..| +FC12044_91407 1 --------------TTTCCCACCC--CAGGAA-GCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 18/61 (29.5%) +# Similarity: 18/61 (29.5%) +# Gaps: 39/61 (63.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC---GACGC 47 + |||||| ||.| ||.|| +FC12044_91407 1 -------------------------------TTTCCC-ACCCCAGGAAGC 18 + +Illumina_Pair 48 TCTTCCGATCT 58 + ||| |..| +FC12044_91407 19 -CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 68 +# Identity: 15/68 (22.1%) +# Similarity: 15/68 (22.1%) +# Gaps: 50/68 (73.5%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACC-- 48 + .|||| | ||| +FC12044_91407 1 ----------------------------------TTTCC--C---ACCCC 11 + +Illumina_Pair 49 -----GCTCTTCCGATCT 61 + || ||| |..| +FC12044_91407 12 AGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 18/36 (50.0%) +# Similarity: 18/36 (50.0%) +# Gaps: 14/36 (38.9%) +# Score: 88.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC---GACGCTCTTCCGATCT 33 + |||||| ||.| ||.|| ||| |..| +FC12044_91407 1 ------TTTCCC-ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 15/44 (34.1%) +# Similarity: 15/44 (34.1%) +# Gaps: 26/44 (59.1%) +# Score: 67.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACC-------GCTCTTCCGATCT 37 + .|||| | ||| || ||| |..| +FC12044_91407 1 ----------TTTCC--C---ACCCCAGGAAGC-CTT--GGAC- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 38.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGT--------CGGACTGTAGAA--CTCTGAAC 26 + | | |.| ||| || ||.|| +FC12044_91407 1 -----TTTCCCACCC---CAG--GAAGCCT-TGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTCAGAGTTCTAC-------AGTCC---GAC 26 + .||| |.|| || || ||| +FC12044_91407 1 ----TTTC------CCACCCCAGGAAG-CCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTAT---GCCGTCTTCTG-----CTTG--- 21 + |.| .|| | |.| |||| +FC12044_91407 1 ---TTTCCCACC--C--CAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTT-CAGAGTTCT---ACAGTCCGA 44 + .|.| ||||| .||||.. | || || +FC12044_91407 1 ----TTTC-----CCACC-CCAGGAAGC------CTTGGAC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 31/44 (70.5%) +# Score: 44.0 +# +# +#======================================= + +Illumina_DpnI 1 ---------CGACAGGTTCAGAGTTCTACAGTCC---GACGATC 32 + | ||||. || || ||| +FC12044_91407 1 TTTCCCACCC--CAGGA--AG-----------CCTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 12/33 (36.4%) +# Similarity: 12/33 (36.4%) +# Gaps: 20/33 (60.6%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 --TC--------GGA--CTGTAGAACTCTGAAC 21 + || ||| | || ||.|| +FC12044_91407 1 TTTCCCACCCCAGGAAGC-------CT-TGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 13/38 (34.2%) +# Similarity: 13/38 (34.2%) +# Gaps: 22/38 (57.9%) +# Score: 44.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCC---GA---CATG--- 29 + .||| |.|| || || |.|| +FC12044_91407 1 ----TTTC------CCAC---CCCAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 20/34 (58.8%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 ---------CAAGCAGAAGACGGCAT--ACGANN 23 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTAT---GCCGTCTTCTG-----CTTG--- 21 + |.| .|| | |.| |||| +FC12044_91407 1 ---TTTCCCACC--C--CAGGAAGCCTTGGAC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_NlaI 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTT-CAGAGTTCT---ACAGTCCGA 44 + .|.| ||||| .||||.. | || || +FC12044_91407 1 ----TTTC-----CCACC-CCAGGAAGC------CTTGGAC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 13/43 (30.2%) +# Similarity: 13/43 (30.2%) +# Gaps: 29/43 (67.4%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 --------CCGACAGGTTCAGAGTTCTACAGTCC---GACATG 32 + || ||||. || || ||| +FC12044_91407 1 TTTCCCACCC--CAGGA--AG-----------CCTTGGAC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 13/36 (36.1%) +# Similarity: 13/36 (36.1%) +# Gaps: 21/36 (58.3%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Smal 1 GTTCAGAGTTCTAC-------AGTCC---GACGATC 26 + .||| |.|| || || ||| +FC12044_91407 1 TTTC------CCACCCCAGGAAG-CCTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 9/35 (25.7%) +# Similarity: 9/35 (25.7%) +# Gaps: 23/35 (65.7%) +# Score: 38.5 +# +# +#======================================= + +Illumina_Smal 1 --TC--------GTATGCC---GTCTTCTGCTTGT 22 + || |.|.||| |.| +FC12044_91407 1 TTTCCCACCCCAGGAAGCCTTGGAC---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 10/32 (31.2%) +# Similarity: 10/32 (31.2%) +# Gaps: 18/32 (56.2%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 ---------CAAGCAGAAGACGGCAT--ACGA 21 + | |||.|..| |.| || +FC12044_91407 1 TTTCCCACCC---CAGGAAGC--CTTGGAC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 48 +# Identity: 16/48 (33.3%) +# Similarity: 16/48 (33.3%) +# Gaps: 27/48 (56.2%) +# Score: 68.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTT-CAGAGTTCT---ACAGTCCGA 44 + .|.| ||||| .||||.. | || || +FC12044_91407 1 ----TTTC-----CCACC-CCAGGAAGC------CTTGGAC------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_8_865 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 44 +# Identity: 12/44 (27.3%) +# Similarity: 12/44 (27.3%) +# Gaps: 31/44 (70.5%) +# Score: 44.0 +# +# +#======================================= + +Illumina_Smal 1 ---------CGACAGGTTCAGAGTTCTACAGTCC---GACGATC 32 + | ||||. || || ||| +FC12044_91407 1 TTTCCCACCC--CAGGA--AG-----------CCTTGGAC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 17/37 (45.9%) +# Similarity: 17/37 (45.9%) +# Gaps: 16/37 (43.2%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAG-CT-CGTATGCC--GTCTTCTGCTTG 33 + || || || || |||| |.|..||.| +FC12044_91407 1 --TC-----AGCCTCCG--TGCCCAGCCCACTCC--- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Geno 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 58 +# Identity: 18/58 (31.0%) +# Similarity: 18/58 (31.0%) +# Gaps: 33/58 (56.9%) +# Score: 98.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + .|.| ||.|||.| |||..|.| || +FC12044_91407 1 ------TCAG---CCTCCGTG-------------CCCAGCCC-AC----- 22 + +Illumina_Geno 51 TCCGATCT 58 + ||| +FC12044_91407 23 TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 14/36 (38.9%) +# Similarity: 14/36 (38.9%) +# Gaps: 13/36 (36.1%) +# Score: 68.0 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT-- 34 + .|.||.|.||.||.|..||.| || +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCA-CTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Geno 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 12/43 (27.9%) +# Similarity: 12/43 (27.9%) +# Gaps: 29/43 (67.4%) +# Score: 53.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAG---CAGGAATGCCGAG-------- 32 + |||| |.| ||||.|| +FC12044_91407 1 ---------------TCAGCCTCCG---TGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 58 +# Identity: 18/58 (31.0%) +# Similarity: 18/58 (31.0%) +# Gaps: 33/58 (56.9%) +# Score: 98.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCT 50 + .|.| ||.|||.| |||..|.| || +FC12044_91407 1 ------TCAG---CCTCCGTG-------------CCCAGCCC-AC----- 22 + +Illumina_Pair 51 TCCGATCT 58 + ||| +FC12044_91407 23 TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 63 +# Identity: 19/63 (30.2%) +# Similarity: 19/63 (30.2%) +# Gaps: 40/63 (63.5%) +# Score: 99.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCT-CG-GCATTCCTGCTGAACC 48 + ||.|.|| || || ||.|| || +FC12044_91407 1 -----------------------TCAGCCTCCGTGC---CCAGC----CC 20 + +Illumina_Pair 49 GCTCTTCCGATCT 61 + .||| | +FC12044_91407 21 ACTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 16/36 (44.4%) +# Similarity: 16/36 (44.4%) +# Gaps: 14/36 (38.9%) +# Score: 78.5 +# +# +#======================================= + +Illumina_Pair 1 ACA--CT-CTTTCCCTACACGACGCTCTTCCGATCT 33 + .|| || |.|.|||..|.| || ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCC-AC-----TCC----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 18/40 (45.0%) +# Similarity: 18/40 (45.0%) +# Gaps: 18/40 (45.0%) +# Score: 92.0 +# +# +#======================================= + +Illumina_Pair 1 -CGGTCT-CG-GCATTCCTGCTGAACCGCTCTTCCGATCT 37 + |.|.|| || || ||.|| ||.||| | +FC12044_91407 1 TCAGCCTCCGTGC---CCAGC----CCACTC--C------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 50.0 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACT--GT----AG--AACTCTGAAC 26 + ||.|.|| || || .|||| | +FC12044_91407 1 -----TCAGCCTCCGTGCCCAGCCCACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 14/34 (41.2%) +# Similarity: 14/34 (41.2%) +# Gaps: 17/34 (50.0%) +# Score: 53.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAG-----GTTCAGAGTTCTACAGTCCGAC--- 26 + .||| ||.| | |||.|| || +FC12044_91407 1 TCAGCCTCCGTGC------C--CAGCCC-ACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 43.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCCGTCTTCTGCTTG------------- 21 + || .||| || |.|| +FC12044_91407 1 TC---AGCC-TC-----CGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 17/47 (36.2%) +# Similarity: 17/47 (36.2%) +# Gaps: 25/47 (53.2%) +# Score: 78.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGA---CAGGTTCAGAGTTCTACAGTCCGA 44 + .|.| ||.|||. ||| | |.|| ||| +FC12044_91407 1 ------TCAG---CCTCCGTGCCCAG---C------CCAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 16/38 (42.1%) +# Similarity: 16/38 (42.1%) +# Gaps: 19/38 (50.0%) +# Score: 59.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAG-----GTTCAGAGTTCTACAGTCCGACGATC- 32 + .||| ||.| | |||.|| || || +FC12044_91407 1 --TCAGCCTCCGTGC------C--CAGCCC-AC--TCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 29 +# Identity: 14/29 (48.3%) +# Similarity: 14/29 (48.3%) +# Gaps: 12/29 (41.4%) +# Score: 50.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGGACT--GT----AG--AACTCTGAAC 21 + ||.|.|| || || .|||| | +FC12044_91407 1 TCAGCCTCCGTGCCCAGCCCACTC----C 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 12/42 (28.6%) +# Similarity: 12/42 (28.6%) +# Gaps: 30/42 (71.4%) +# Score: 51.5 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTCAGAGTTCTACAGTCCGACATG------------- 29 + |||| | | |||| || +FC12044_91407 1 ------TCAG----C--C--TCCG---TGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 9/37 (24.3%) +# Similarity: 9/37 (24.3%) +# Gaps: 26/37 (70.3%) +# Score: 30.0 +# +# +#======================================= + +Illumina_NlaI 1 -------------CAAGCAGAAGACGGCA-TACGANN 23 + |.||| | || |.| +FC12044_91407 1 TCAGCCTCCGTGCCCAGC------C--CACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 10/34 (29.4%) +# Similarity: 10/34 (29.4%) +# Gaps: 22/34 (64.7%) +# Score: 43.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCCGTCTTCTGCTTG------------- 21 + || .||| || |.|| +FC12044_91407 1 TC---AGCC-TC-----CGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 17/47 (36.2%) +# Similarity: 17/47 (36.2%) +# Gaps: 25/47 (53.2%) +# Score: 78.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGA---CAGGTTCAGAGTTCTACAGTCCGA 44 + .|.| ||.|||. ||| | |.|| ||| +FC12044_91407 1 ------TCAG---CCTCCGTGCCCAG---C------CCAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 42 +# Identity: 13/42 (31.0%) +# Similarity: 13/42 (31.0%) +# Gaps: 27/42 (64.3%) +# Score: 57.0 +# +# +#======================================= + +Illumina_NlaI 1 -------CCGA---CAGGTTCAGAGTTCTACAGTCCGACATG 32 + |||. ||| | |.|| ||| +FC12044_91407 1 TCAGCCTCCGTGCCCAG---C------CCAC--TCC------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 16/31 (51.6%) +# Similarity: 16/31 (51.6%) +# Gaps: 11/31 (35.5%) +# Score: 59.0 +# +# +#======================================= + +Illumina_Smal 1 GTTCAG----AGTTCTACAGTCCGACGATC- 26 + |||| .||.| .|||.|| || || +FC12044_91407 1 --TCAGCCTCCGTGC-CCAGCCC-AC--TCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 12/31 (38.7%) +# Similarity: 12/31 (38.7%) +# Gaps: 15/31 (48.4%) +# Score: 42.0 +# +# +#======================================= + +Illumina_Smal 1 ------T-CGTATGCC--GTCTTCTGCTTGT 22 + | || |||| |.|..||.| +FC12044_91407 1 TCAGCCTCCG--TGCCCAGCCCACTCC---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 6/35 (17.1%) +# Similarity: 6/35 (17.1%) +# Gaps: 24/35 (68.6%) +# Score: 33.0 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + .|.||.|.||. +FC12044_91407 1 ----------TCAGCCTCCGTGCCCAGCCCACTCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 47 +# Identity: 17/47 (36.2%) +# Similarity: 17/47 (36.2%) +# Gaps: 25/47 (53.2%) +# Score: 78.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGA---CAGGTTCAGAGTTCTACAGTCCGA 44 + .|.| ||.|||. ||| | |.|| ||| +FC12044_91407 1 ------TCAG---CCTCCGTGCCCAG---C------CCAC--TCC-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_292_484 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 38 +# Identity: 16/38 (42.1%) +# Similarity: 16/38 (42.1%) +# Gaps: 19/38 (50.0%) +# Score: 59.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAG-----GTTCAGAGTTCTACAGTCCGACGATC- 32 + .||| ||.| | |||.|| || || +FC12044_91407 1 --TCAGCCTCCGTGC------C--CAGCCC-AC--TCC 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 12/43 (27.9%) +# Similarity: 12/43 (27.9%) +# Gaps: 28/43 (65.1%) +# Score: 44.0 +# +# +#======================================= + +Illumina_Geno 1 GATCGGAAGAGCT----C------GTATGCCGTCTTCTGCTTG 33 + .||||.|| ||| | ||. +FC12044_91407 1 -CTCGGGAG-GCTGAGGCAGGGGGGTT---------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 71 +# Identity: 10/71 (14.1%) +# Similarity: 10/71 (14.1%) +# Gaps: 59/71 (83.1%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC--GACGCT 48 + |.| ||.||| +FC12044_91407 1 ---------------------------------------CTCGGGAGGCT 11 + +Illumina_Geno 49 ----C-------TTCCGATCT 58 + | || +FC12044_91407 12 GAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 14/37 (37.8%) +# Similarity: 14/37 (37.8%) +# Gaps: 15/37 (40.5%) +# Score: 43.5 +# +# +#======================================= + +Illumina_Geno 1 CAAGCAGAAGAC---GGCATACGAGCTCTTCCGATCT 34 + |. |.|.||.| ||||...|.| || +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGG---TT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 16/35 (45.7%) +# Similarity: 16/35 (45.7%) +# Gaps: 13/35 (37.1%) +# Score: 57.0 +# +# +#======================================= + +Illumina_Pair 1 GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG--- 32 + .||||.|| ||.|. .||||| |.| +FC12044_91407 1 -CTCGGGAG-GCTGA--GGCAGG------GGGGTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 71 +# Identity: 10/71 (14.1%) +# Similarity: 10/71 (14.1%) +# Gaps: 59/71 (83.1%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACAC--GACGCT 48 + |.| ||.||| +FC12044_91407 1 ---------------------------------------CTCGGGAGGCT 11 + +Illumina_Pair 49 ----C-------TTCCGATCT 58 + | || +FC12044_91407 12 GAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 69 +# Identity: 14/69 (20.3%) +# Similarity: 14/69 (20.3%) +# Gaps: 52/69 (75.4%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + |||||.| .|||||. || +FC12044_91407 1 ----------------------------CTCGGGA----GGCTGAG--GC 16 + +Illumina_Pair 51 --------TCTTCCGATCT 61 + | +FC12044_91407 17 AGGGGGGTT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 46 +# Identity: 10/46 (21.7%) +# Similarity: 10/46 (21.7%) +# Gaps: 34/46 (73.9%) +# Score: 34.0 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC--GACGCT----C-------TTCCGATCT 33 + |.| ||.||| | || +FC12044_91407 1 --------------CTCGGGAGGCTGAGGCAGGGGGGTT------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 45 +# Identity: 14/45 (31.1%) +# Similarity: 14/45 (31.1%) +# Gaps: 28/45 (62.2%) +# Score: 50.5 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGCATTCCTGCTGAACCGC--------TCTTCCGATCT 37 + |||||.| .|||||. || | +FC12044_91407 1 ----CTCGGGA----GGCTGAG--GCAGGGGGGTT---------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 36 +# Identity: 12/36 (33.3%) +# Similarity: 12/36 (33.3%) +# Gaps: 21/36 (58.3%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 GATCGTCGGACTGTAGAACTCTGA-AC--------- 26 + | |||| |..| |||| .| +FC12044_91407 1 ---C-TCGG---GAGG----CTGAGGCAGGGGGGTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 8/40 (20.0%) +# Similarity: 8/40 (20.0%) +# Gaps: 29/40 (72.5%) +# Score: 37.0 +# +# +#======================================= + +Illumina_DpnI 1 --------------ACAGGTTCAGAGTTCTACAGTCCGAC 26 + .|||| .|.||| +FC12044_91407 1 CTCGGGAGGCTGAGGCAGG---GGGGTT------------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 27.0 +# +# +#======================================= + +Illumina_DpnI 1 -TCGTATGCCGTCTTCT---GC-------TTG 21 + |||...| | || || || +FC12044_91407 1 CTCGGGAG--G----CTGAGGCAGGGGGGTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_DpnI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + | ||| || |.|||| .|.||| +FC12044_91407 1 -------CTCGGGAGGCTGA-----GGCAGG---GGGGTT---------- 25 + +Illumina_DpnI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 42.5 +# +# +#======================================= + +Illumina_DpnI 1 --CGACAGGTTCAGAGTTCTACAG------TCCGACGATC 32 + ||..||| |.||| .||| | +FC12044_91407 1 CTCGGGAGG--CTGAG----GCAGGGGGGTT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 -TCGGACTGTAGAACTCTGA-AC--------- 21 + |||| |..| |||| .| +FC12044_91407 1 CTCGG---GAGG----CTGAGGCAGGGGGGTT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 8/43 (18.6%) +# Similarity: 8/43 (18.6%) +# Gaps: 32/43 (74.4%) +# Score: 37.0 +# +# +#======================================= + +Illumina_NlaI 1 --------------ACAGGTTCAGAGTTCTACAGTCCGACATG 29 + .|||| .|.||| +FC12044_91407 1 CTCGGGAGGCTGAGGCAGG---GGGGTT--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 18/33 (54.5%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC---GGCA-------TACGANN 23 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 11/32 (34.4%) +# Similarity: 11/32 (34.4%) +# Gaps: 18/32 (56.2%) +# Score: 27.0 +# +# +#======================================= + +Illumina_NlaI 1 -TCGTATGCCGTCTTCT---GC-------TTG 21 + |||...| | || || || +FC12044_91407 1 CTCGGGAG--G----CTGAGGCAGGGGGGTT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_NlaI 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + | ||| || |.|||| .|.||| +FC12044_91407 1 -------CTCGGGAGGCTGA-----GGCAGG---GGGGTT---------- 25 + +Illumina_NlaI 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 43 +# Identity: 11/43 (25.6%) +# Similarity: 11/43 (25.6%) +# Gaps: 29/43 (67.4%) +# Score: 42.5 +# +# +#======================================= + +Illumina_NlaI 1 ---------CCGA--CAGGTTCAGAGTTCTACAGTCCGACATG 32 + |.|| |||| .|.||| +FC12044_91407 1 CTCGGGAGGCTGAGGCAGG---GGGGTT--------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 9/40 (22.5%) +# Similarity: 9/40 (22.5%) +# Gaps: 29/40 (72.5%) +# Score: 25.5 +# +# +#======================================= + +Illumina_Smal 1 --------GTTCAGAGTTCTACAG------TCCGACGATC 26 + | |.||| .||| | +FC12044_91407 1 CTCGGGAGG--CTGAG----GCAGGGGGGTT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 19/33 (57.6%) +# Score: 27.0 +# +# +#======================================= + +Illumina_Smal 1 -TCGTATGCCGTCTTCT---GC-------TTGT 22 + |||...| | || || || +FC12044_91407 1 CTCGGGAG--G----CTGAGGCAGGGGGGTT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 11/31 (35.5%) +# Similarity: 11/31 (35.5%) +# Gaps: 16/31 (51.6%) +# Score: 41.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGAC---GGCA-------TACGA 21 + |. |.|.||.| |||| | +FC12044_91407 1 CT--CGGGAGGCTGAGGCAGGGGGGTT---- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 51 +# Identity: 15/51 (29.4%) +# Similarity: 15/51 (29.4%) +# Gaps: 33/51 (64.7%) +# Score: 52.5 +# +# +#======================================= + +Illumina_Smal 1 AATGATAC------GGC-GACCACCGACAGGTTCAGAGTTCTACAGTCCG 43 + | ||| || |.|||| .|.||| +FC12044_91407 1 -------CTCGGGAGGCTGA-----GGCAGG---GGGGTT---------- 25 + +Illumina_Smal 44 A 44 + +FC12044_91407 26 - 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_675_16 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 23/40 (57.5%) +# Score: 42.5 +# +# +#======================================= + +Illumina_Smal 1 --CGACAGGTTCAGAGTTCTACAG------TCCGACGATC 32 + ||..||| |.||| .||| | +FC12044_91407 1 CTCGGGAGG--CTGAG----GCAGGGGGGTT--------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomici_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 16/40 (40.0%) +# Similarity: 16/40 (40.0%) +# Gaps: 22/40 (55.0%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 ---GATC-GGAA--G-AGCTCGTATGCCGTCTTCTGCTTG 33 + .||| .||| | ||||| || || +FC12044_91407 1 CCAAATCTTGAATTGTAGCTC-----CC--CT-------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 65 +# Identity: 15/65 (23.1%) +# Similarity: 15/65 (23.1%) +# Gaps: 47/65 (72.3%) +# Score: 80.0 +# +# +#======================================= + +Illumina_Geno 1 AATGATACGGCGACCACCGAGATCTACA-------CTCTTTCCCTACACG 43 + || |.||||..| ||| |||| +FC12044_91407 1 ----------------CC-AAATCTTGAATTGTAGCTC---CCCT----- 25 + +Illumina_Geno 44 ACGCTCTTCCGATCT 58 + +FC12044_91407 26 --------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 17/39 (43.6%) +# Similarity: 17/39 (43.6%) +# Gaps: 19/39 (48.7%) +# Score: 62.0 +# +# +#======================================= + +Illumina_Geno 1 -CAAG-C-AGAA--GACGGCATACGAGCTCTTCCGATCT 34 + |||. | .||| |. ||||| || || +FC12044_91407 1 CCAAATCTTGAATTGT---------AGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Genomic_DNA_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Geno 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 39 +# Identity: 15/39 (38.5%) +# Similarity: 15/39 (38.5%) +# Gaps: 21/39 (53.8%) +# Score: 37.0 +# +# +#======================================= + +Illumina_Pair 1 ---GATC-GGAA--G-AGCGGTTCAGCAGGAATGCCGAG 32 + .||| .||| | ||| || | ||. +FC12044_91407 1 CCAAATCTTGAATTGTAGC---TC--C-------CCT-- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 65 +# Identity: 15/65 (23.1%) +# Similarity: 15/65 (23.1%) +# Gaps: 47/65 (72.3%) +# Score: 80.0 +# +# +#======================================= + +Illumina_Pair 1 AATGATACGGCGACCACCGAGATCTACA-------CTCTTTCCCTACACG 43 + || |.||||..| ||| |||| +FC12044_91407 1 ----------------CC-AAATCTTGAATTGTAGCTC---CCCT----- 25 + +Illumina_Pair 44 ACGCTCTTCCGATCT 58 + +FC12044_91407 26 --------------- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_PCR_Primers1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 61 +# Identity: 21/61 (34.4%) +# Similarity: 21/61 (34.4%) +# Gaps: 36/61 (59.0%) +# Score: 74.0 +# +# +#======================================= + +Illumina_Pair 1 CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGC 50 + | ||.| |||.| |.| ||.| || +FC12044_91407 1 -----------C--CAAA---------TCTTG--AAT-----TGTA--GC 19 + +Illumina_Pair 51 TCTTCCGATCT 61 + || || || +FC12044_91407 20 TC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 40 +# Identity: 13/40 (32.5%) +# Similarity: 13/40 (32.5%) +# Gaps: 22/40 (55.0%) +# Score: 62.5 +# +# +#======================================= + +Illumina_Pair 1 ACACTCTTTCCCTACAC-------GACGCTCTTCCGATCT 33 + ||.|..| |..|||| || || +FC12044_91407 1 ----------CCAAATCTTGAATTGTAGCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Paired_End_DNA_sequencing_primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 41 +# Identity: 17/41 (41.5%) +# Similarity: 17/41 (41.5%) +# Gaps: 20/41 (48.8%) +# Score: 66.0 +# +# +#======================================= + +Illumina_Pair 1 CGGTCTCGGC--ATTCCTGC--TGAACCGCTCTTCCGATCT 37 + | |.||.||. ||.| |||| || || +FC12044_91407 1 ---------CCAAATCTTGAATTGTA--GCTC--CC---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 15/32 (46.9%) +# Similarity: 15/32 (46.9%) +# Gaps: 13/32 (40.6%) +# Score: 61.0 +# +# +#======================================= + +Illumina_DpnI 1 ---GATCGTCGGACTGTAGAACTCTGAAC--- 26 + .|||.| |.|.||||| ||| | +FC12044_91407 1 CCAAATCTT-GAATTGTAG--CTC----CCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 30 +# Identity: 14/30 (46.7%) +# Similarity: 14/30 (46.7%) +# Gaps: 9/30 (30.0%) +# Score: 49.0 +# +# +#======================================= + +Illumina_DpnI 1 ACAGGTTC-AGAGTTCTA-CAGTCCGAC-- 26 + |....|| .||.||.|| | ||| | +FC12044_91407 1 -CCAAATCTTGAATTGTAGC--TCC--CCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 8/37 (21.6%) +# Similarity: 8/37 (21.6%) +# Gaps: 28/37 (75.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_DpnI 1 TCGTATGCC--GTCTT--------------CTGCTTG 21 + || .|||| || +FC12044_91407 1 -------CCAAATCTTGAATTGTAGCTCCCCT----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_DpnI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 14/49 (28.6%) +# Similarity: 14/49 (28.6%) +# Gaps: 29/49 (59.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_DpnI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTT----CTACAGTCCGA 44 + ||.| .|| .||.|| ||.| ||. +FC12044_91407 1 ----------------CCAA----ATCTTGAATTGTAGCTCC---CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_DpnII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 17/35 (48.6%) +# Similarity: 17/35 (48.6%) +# Gaps: 13/35 (37.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_DpnI 1 CGACAGGTTC-AGAGTTCTA-CAGTCCGACGATC- 32 + | || ..|| .||.||.|| | ||| | | +FC12044_91407 1 C--CA-AATCTTGAATTGTAGC--TCC--C---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 13/31 (41.9%) +# Similarity: 13/31 (41.9%) +# Gaps: 16/31 (51.6%) +# Score: 55.5 +# +# +#======================================= + +Illumina_NlaI 1 -----TC--GGACTGTAGAACTCTGAAC--- 21 + || |.|.||||| ||| | +FC12044_91407 1 CCAAATCTTGAATTGTAG--CTC----CCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters1_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 31 +# Identity: 15/31 (48.4%) +# Similarity: 15/31 (48.4%) +# Gaps: 8/31 (25.8%) +# Score: 50.0 +# +# +#======================================= + +Illumina_NlaI 1 ACAGGTTC-AGAGTTCTA-CAGTCCGACATG 29 + |....|| .||.||.|| | ||| |.| +FC12044_91407 1 -CCAAATCTTGAATTGTAGC--TCC--CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 33 +# Identity: 11/33 (33.3%) +# Similarity: 11/33 (33.3%) +# Gaps: 18/33 (54.5%) +# Score: 20.0 +# +# +#======================================= + +Illumina_NlaI 1 -CAAG-C-AGAA--GA-----CGGCATACGANN 23 + |||. | .||| |. | |.| +FC12044_91407 1 CCAAATCTTGAATTGTAGCTCC--CCT------ 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_Adapters2_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 37 +# Identity: 8/37 (21.6%) +# Similarity: 8/37 (21.6%) +# Gaps: 28/37 (75.7%) +# Score: 39.0 +# +# +#======================================= + +Illumina_NlaI 1 TCGTATGCC--GTCTT--------------CTGCTTG 21 + || .|||| || +FC12044_91407 1 -------CCAAATCTTGAATTGTAGCTCCCCT----- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_NlaI 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_PCR_Primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 14/49 (28.6%) +# Similarity: 14/49 (28.6%) +# Gaps: 29/49 (59.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_NlaI 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTT----CTACAGTCCGA 44 + ||.| .|| .||.|| ||.| ||. +FC12044_91407 1 ----------------CCAA----ATCTTGAATTGTAGCTCC---CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_NlaIII_Gex_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 17/34 (50.0%) +# Similarity: 17/34 (50.0%) +# Gaps: 11/34 (32.4%) +# Score: 62.5 +# +# +#======================================= + +Illumina_NlaI 1 CCGACAGGTTC-AGAGTTCTA-CAGTCCGACATG 32 + ||.| .|| .||.||.|| | ||| |.| +FC12044_91407 1 CCAA----ATCTTGAATTGTAGC--TCC--CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_RT_Primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_5p_Adapter +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 32 +# Identity: 14/32 (43.8%) +# Similarity: 14/32 (43.8%) +# Gaps: 13/32 (40.6%) +# Score: 46.0 +# +# +#======================================= + +Illumina_Smal 1 ---GTTC-AGAGTTCTA-CAGTCCGACGATC- 26 + ..|| .||.||.|| | ||| | | +FC12044_91407 1 CCAAATCTTGAATTGTAGC--TCC--C---CT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_3p_Adapter +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 34 +# Identity: 11/34 (32.4%) +# Similarity: 11/34 (32.4%) +# Gaps: 21/34 (61.8%) +# Score: 45.0 +# +# +#======================================= + +Illumina_Smal 1 TCGTATGCC--GTCTTCTG-CTTGT--------- 22 + || .||| || .|||| +FC12044_91407 1 -------CCAAATCT--TGAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_1 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 8/35 (22.9%) +# Similarity: 8/35 (22.9%) +# Gaps: 24/35 (68.6%) +# Score: 23.5 +# +# +#======================================= + +Illumina_Smal 1 CAAGCAGAAGACGGCATACGA-------------- 21 + | ||.|. |.| || +FC12044_91407 1 C---CAAAT-----CTT--GAATTGTAGCTCCCCT 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_PCR_Primer_2 +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 49 +# Identity: 14/49 (28.6%) +# Similarity: 14/49 (28.6%) +# Gaps: 29/49 (59.2%) +# Score: 54.0 +# +# +#======================================= + +Illumina_Smal 1 AATGATACGGCGACCACCGACAGGTTC-AGAGTT----CTACAGTCCGA 44 + ||.| .|| .||.|| ||.| ||. +FC12044_91407 1 ----------------CCAA----ATCTTGAATTGTAGCTCC---CCT- 25 + + +#======================================= +# +# Aligned_sequences: 2 +# 1: Illumina_Small_RNA_sequencing_primer +# 2: FC12044_91407_8_200_285_136 +# Matrix: EPAM30 +# Gap_penalty: 10.0 +# Extend_penalty: 0.5 +# +# Length: 35 +# Identity: 17/35 (48.6%) +# Similarity: 17/35 (48.6%) +# Gaps: 13/35 (37.1%) +# Score: 51.5 +# +# +#======================================= + +Illumina_Smal 1 CGACAGGTTC-AGAGTTCTA-CAGTCCGACGATC- 32 + | || ..|| .||.||.|| | ||| | | +FC12044_91407 1 C--CA-AATCTTGAATTGTAGC--TCC--C---CT 25 + + +#--------------------------------------- +#---------------------------------------