Mercurial > repos > iuc > ena_upload
comparison ena_upload.xml @ 0:382518f24d6d draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ena_upload commit 57b434bcf493554d060a99b65e66f274d5c00e0a"
author | iuc |
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date | Sat, 28 Nov 2020 09:45:44 +0000 |
parents | |
children | 57251c760cab |
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-1:000000000000 | 0:382518f24d6d |
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1 <tool id="ena_upload" name="ENA Upload tool" version="0.3" profile="20.01" license="MIT"> | |
2 <macros> | |
3 <token name="@VERSION@">0.2.4</token> | |
4 <import>samples_macros.xml</import> | |
5 </macros> | |
6 <requirements> | |
7 <requirement type="package" version="@VERSION@">ena-upload-cli</requirement> | |
8 <requirement type="package" version="1.2.0">xlrd</requirement> | |
9 </requirements> | |
10 <command detect_errors="exit_code"><![CDATA[ | |
11 mkdir ./submission_files; | |
12 | |
13 #set working_dir = os.getcwd() | |
14 #set $dry_run_option = "False" | |
15 #set viral_submission = "False" | |
16 #if $action_options.input_format_conditional.input_format == "build_tables": | |
17 python '$__tool_directory__/extract_tables.py' --action $action_options.action --out_dir ./submission_files --studies $studies_json; | |
18 #set $studies_table_path = './submission_files/studies.tsv' | |
19 #set $samples_table_path = './submission_files/samples.tsv' | |
20 #set $experiments_table_path = './submission_files/experiments.tsv' | |
21 #set $runs_table_path = './submission_files/runs.tsv' | |
22 #end if | |
23 | |
24 #if $action_options.input_format_conditional.input_format == "excel_tables": | |
25 python '$__tool_directory__/process_xlsx.py' | |
26 #if $action_options.input_format_conditional.viral_submission == "true": | |
27 --vir | |
28 #end if | |
29 --action '$action_options.action' --form '$action_options.input_format_conditional.xlsx_file' --out_dir ./submission_files ; | |
30 #set $studies_table_path = './submission_files/studies.tsv' | |
31 #set $samples_table_path = './submission_files/samples.tsv' | |
32 #set $experiments_table_path = './submission_files/experiments.tsv' | |
33 #set $runs_table_path = './submission_files/runs.tsv' | |
34 #if $action_options.input_format_conditional.dry_run == "true": | |
35 #set $dry_run_option = "True" | |
36 #end if | |
37 #end if | |
38 | |
39 #if $action_options.input_format_conditional.input_format != "user_generated_tables": | |
40 cp $studies_table_path $studies_table_out; | |
41 cp $samples_table_path $samples_table_out; | |
42 cp $experiments_table_path $experiments_table_out; | |
43 cp $runs_table_path $runs_table_out; | |
44 #if $action_options.input_format_conditional.dry_run == "true": | |
45 #set $dry_run_option = "True" | |
46 #end if | |
47 #end if | |
48 | |
49 | |
50 #if $dry_run_option == "False" and $action_options.test_submit == "False": | |
51 webin_id=`grep 'username' $credentials`; | |
52 if [ "\$webin_id" = "" ]; then | |
53 ## No credentials in user defined preferences | |
54 ## Fallback to global defined credentials (if exist) | |
55 #import os | |
56 #if os.path.isfile(os.environ.get('GALAXY_ENA_SECRETS', '')): | |
57 credentials_path=\${ENA_SECRETS}; | |
58 webin_id=`grep 'username' \$GALAXY_ENA_SECRETS`; | |
59 if [ "\$webin_id" = "" ]; then | |
60 echo "No global credentials defined. Check your GALAXY_ENA_SECRETS file or set your credentials via: User -> Preferences -> Manage Information"; | |
61 exit 1; | |
62 fi; | |
63 #else: | |
64 echo "No ENA credentials defined. Set your credentials via: User -> Preferences -> Manage Information"; | |
65 exit 1; | |
66 #end if | |
67 else | |
68 credentials_path='$credentials'; | |
69 fi; | |
70 #end if | |
71 | |
72 #if $action_options.test_submit == "True": | |
73 credentials_path='test_fake_path'; | |
74 #end if | |
75 | |
76 ## create the list of files to upload and make the symlinks | |
77 #import re | |
78 #set $files_to_upload = list() | |
79 #if $action_options.input_format_conditional.input_format == "build_tables": | |
80 #for $study in $action_options.input_format_conditional.conditional_viral_metadata.rep_study: | |
81 #for $sample in $study.rep_sample: | |
82 #for $experiment in $sample.rep_experiment: | |
83 #for $run in $experiment.rep_runs: | |
84 #for $file in $run.upload_files: | |
85 #set $safename_reads_file = re.sub('[^\w\-_\.]', '_', $file.element_identifier) | |
86 ln -s '$file' $safename_reads_file && | |
87 $files_to_upload.append(str($safename_reads_file)) | |
88 #end for | |
89 #end for | |
90 #end for | |
91 #end for | |
92 #end for | |
93 #else: | |
94 #for $file in $action_options.input_format_conditional.data: | |
95 #set $safename_reads_file = re.sub('[^\w\-_\.]', '_', $file.element_identifier) | |
96 ln -s '$file' $safename_reads_file && | |
97 $files_to_upload.append(str($safename_reads_file)) | |
98 #end for | |
99 #end if | |
100 | |
101 | |
102 #if $dry_run_option == "False": | |
103 ena-upload-cli | |
104 --tool 'ena-upload-cli v@VERSION@ @ Galaxy' | |
105 --action '$action_options.action' | |
106 --center '$action_options.center' | |
107 --secret \${credentials_path} | |
108 --data | |
109 #for $dataset in $files_to_upload: | |
110 '$dataset' | |
111 #end for | |
112 #if $action_options.input_format_conditional.input_format == "user_generated_tables": | |
113 --experiment '$action_options.input_format_conditional.experiments_users_table' | |
114 --study '$action_options.input_format_conditional.studies_users_table' | |
115 --run '$action_options.input_format_conditional.runs_users_table' | |
116 --sample '$action_options.input_format_conditional.samples_users_table' | |
117 #if "$action_options.input_format_conditional.viral_submission" == "true": | |
118 --vir | |
119 #end if | |
120 #else: | |
121 --experiment '$experiments_table_path' | |
122 --study '$studies_table_path' | |
123 --run '$runs_table_path' | |
124 --sample '$samples_table_path' | |
125 #if $action_options.input_format_conditional.input_format == "build_tables": | |
126 #if $action_options.input_format_conditional.conditional_viral_metadata.viral_sample == "true": | |
127 --vir | |
128 #end if | |
129 #else: | |
130 #if $action_options.input_format_conditional.viral_submission == "true": | |
131 --vir | |
132 #end if | |
133 #end if | |
134 #end if | |
135 | |
136 #if $action_options.submit_dev == "true": | |
137 -d | |
138 #end if | |
139 > '$output' | |
140 #else: | |
141 echo "" | |
142 exit 0; | |
143 #end if | |
144 | |
145 ]]></command> | |
146 <configfiles> | |
147 <configfile name="credentials"><![CDATA[ | |
148 #set $webin_id = $__user__.extra_preferences.get('ena_webin_account|webin_id', "").strip() | |
149 #set $password = $__user__.extra_preferences.get('ena_webin_account|password', "").strip() | |
150 #if $webin_id != "": | |
151 username: "$webin_id" | |
152 password: "$password" | |
153 #end if | |
154 ]]></configfile> | |
155 <configfile name="studies_json"> | |
156 #import json | |
157 #import re | |
158 #if $action_options.input_format_conditional.input_format == "build_tables": | |
159 #set $files_to_upload = list() | |
160 #set $studies = list() | |
161 #for $study in $action_options.input_format_conditional.conditional_viral_metadata.rep_study: | |
162 #set samples = list() | |
163 #for $sample in $study.rep_sample: | |
164 #set experiments = list() | |
165 #for $experiment in $sample.rep_experiment: | |
166 #set runs = list() | |
167 #for $run in $experiment.rep_runs: | |
168 #set run_files = list() | |
169 #for $file in $run.upload_files: | |
170 #set $safename_reads_file = re.sub('[^\w\-_\.]', '_', $file.element_identifier) | |
171 $run_files.append(str($safename_reads_file)) | |
172 #end for | |
173 $runs.append($run_files) | |
174 #end for | |
175 | |
176 $experiments.append({'title':str($experiment.experiment_title),'experiment_design':str($experiment.experiment_design),'library_strategy':str($experiment.library_strategy),'library_source':str($experiment.library_source),'library_selection':str($experiment.library_selection),'library_layout':str($experiment.library_layout),'insert_size':str($experiment.insert_size),'library_construction_protocol':str($experiment.library_construction_protocol),'platform':str($experiment.platform),'instrument_model':str($experiment.instrument_model),'runs':$runs}) | |
177 #end for | |
178 #if $action_options.input_format_conditional.conditional_viral_metadata.viral_sample == "true": | |
179 | |
180 $samples.append({'title':str($sample.sample_title),'description':str($sample.sample_description),'tax_name':str($sample.scientific_name),'tax_id':str($sample.tax_id),'collection_date':str($sample.collection_date),'geo_location':str($sample.geo_location_country),'host_common_name':str($sample.host_common_name),'host_subject_id':str($sample.host_subject_id),'host_health_state':str($sample.host_health_state),'host_sex':str($sample.host_sex),'host_scientific_name':str($sample.host_scientific_name),'collector_name':str($sample.collector_name),'collecting_institution':str($sample.collecting_institution),'isolate':str($sample.isolate),'experiments':$experiments}) | |
181 #else: | |
182 | |
183 $samples.append({'title':str($sample.sample_title),'description':str($sample.sample_description),'tax_name':str($sample.scientific_name),'tax_id':str($sample.tax_id),'experiments':$experiments}) | |
184 #end if | |
185 #end for | |
186 $studies.append({'title':str($study.study_title),'type':str($study.study_type),'abstract':str($study.study_abstract),'pubmed_id':str($study.study_pubmed_id),'samples':$samples}) | |
187 #end for | |
188 #echo $json.dumps($studies) | |
189 #end if | |
190 </configfile> | |
191 </configfiles> | |
192 <inputs> | |
193 <conditional name="action_options"> | |
194 <param name="action" type="select" label="Action to execute"> | |
195 <option value="add" selected="True">Add new data</option> | |
196 <option value="modify">Modify metadata</option> | |
197 </param> | |
198 <when value="add"> | |
199 <param name="submit_dev" type="boolean" label="Submit to test ENA server?" help="By selecting yes the reads will be submitted " /> | |
200 <param name="test_submit" type="hidden" value="False" /> | |
201 <expand macro="table_inputs_macro" /> | |
202 </when> | |
203 <when value="modify"> | |
204 <expand macro="table_inputs_macro" /> | |
205 </when> | |
206 </conditional> | |
207 </inputs> | |
208 <outputs> | |
209 <data name="output" format="data" label="${tool.name} on ${on_string}: Upload summary"/> | |
210 <data name="studies_table_out" format="tabular" label="Studies table"> | |
211 <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> | |
212 </data> | |
213 <data name="samples_table_out" format="tabular" label="Samples table"> | |
214 <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> | |
215 </data> | |
216 <data name="experiments_table_out" format="tabular" label="Experiments table"> | |
217 <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> | |
218 </data> | |
219 <data name="runs_table_out" format="tabular" label="Runs table"> | |
220 <filter> action_options['input_format_conditional']['input_format'] == "build_tables" or action_options['input_format_conditional']['input_format'] == "excel_tables"</filter> | |
221 </data> | |
222 </outputs> | |
223 <tests> | |
224 <!--Test excel input of VIRAL samples --> | |
225 <test> | |
226 <conditional name="action_options"> | |
227 <param name="action" value="add"/> | |
228 <param name="submit_dev" value="False"/> | |
229 <conditional name="input_format_conditional"> | |
230 <param name="input_format" value="excel_tables"/> | |
231 <param name="viral_submission" value="True"/> | |
232 <param name="dry_run" value="True"/> | |
233 <param name="xlsx_file" value="metadata_test_viral.xlsx"/> | |
234 <param name="data" value="sample.fq"/> | |
235 </conditional> | |
236 </conditional> | |
237 <param name="center" value="Some research center"/> | |
238 <output name="experiments_table_out"> | |
239 <assert_contents> | |
240 <has_n_lines n="5"/> | |
241 <has_n_columns n="17"/> | |
242 <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_alias\tsample_alias\tdesign_description\tlibrary_name\tlibrary_strategy\tlibrary_source\tlibrary_selection\tlibrary_layout\tinsert_size\tlibrary_construction_protocol\tplatform\tinstrument_model\tsubmission_date" /> | |
243 <has_line_matching expression="e_(.*)_026\tadd\taccession_ena\tNanopore sequencing\tSARS-CoV-2_genomes_01\ts_(.*)"/> | |
244 </assert_contents> | |
245 </output> | |
246 <output name="studies_table_out"> | |
247 <assert_contents> | |
248 <has_n_lines n="2"/> | |
249 <has_n_columns n="8"/> | |
250 <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_type\tstudy_abstract\tpubmed_id\tsubmission_date"/> | |
251 <has_line_matching expression="SARS-CoV-2_genomes_01\tadd\tENA_accession\tWhole-genome sequencing of SARS-CoV-2 from Covid-19 patients\tWhole Genome Sequencing\tWhole-genome sequences of SARS-CoV-2 from oro-pharyngeal swabs obtained from Covid-19 patients(.*)"/> | |
252 </assert_contents> | |
253 </output> | |
254 <output name="samples_table_out"> | |
255 <assert_contents> | |
256 <has_n_lines n="5"/> | |
257 <has_n_columns n="18"/> | |
258 <has_line_matching expression="alias\tstatus\taccession\ttitle\tscientific_name\ttaxon_id\tsample_description\tcollection_date\tgeographic_location\thost_common_name\thost_subject_id\thost_health_state\thost_sex\thost_scientific_name\tcollector_name\tcollecting_institution\tisolate\tsubmission_date"/> | |
259 </assert_contents> | |
260 </output> | |
261 <output name="runs_table_out"> | |
262 <assert_contents> | |
263 <has_n_lines n="5"/> | |
264 <has_n_columns n="8"/> | |
265 <has_line_matching expression="alias\tstatus\taccession\texperiment_alias\tfile_name\tfile_format\tfile_checksum\tsubmission_date"/> | |
266 <has_line_matching expression="r_(.*)_026\tadd\tena_run_accession\te_(.*)_026\tC026_exp5_clean.fastq.gz\tfastq\tfile_checksum\tsubmission_date_ENA"/> | |
267 </assert_contents> | |
268 </output> | |
269 </test> | |
270 <!--Test excel input of NON-VIRAL samples--> | |
271 <test> | |
272 <conditional name="action_options"> | |
273 <param name="action" value="add"/> | |
274 <param name="submit_dev" value="False"/> | |
275 <conditional name="input_format_conditional"> | |
276 <param name="input_format" value="excel_tables"/> | |
277 <param name="viral_submission" value="False"/> | |
278 <param name="dry_run" value="True"/> | |
279 <param name="xlsx_file" value="metadata_test_nonviral.xlsx"/> | |
280 <param name="data" value="sample.fq"/> | |
281 </conditional> | |
282 </conditional> | |
283 <param name="center" value="Some research center"/> | |
284 <output name="experiments_table_out"> | |
285 <assert_contents> | |
286 <has_n_lines n="5"/> | |
287 <has_n_columns n="17"/> | |
288 <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_alias\tsample_alias\tdesign_description\tlibrary_name\tlibrary_strategy\tlibrary_source\tlibrary_selection\tlibrary_layout\tinsert_size\tlibrary_construction_protocol\tplatform\tinstrument_model\tsubmission_date" /> | |
289 </assert_contents> | |
290 </output> | |
291 <output name="studies_table_out"> | |
292 <assert_contents> | |
293 <has_n_lines n="2"/> | |
294 <has_n_columns n="8"/> | |
295 <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_type\tstudy_abstract\tpubmed_id\tsubmission_date"/> | |
296 </assert_contents> | |
297 </output> | |
298 <output name="samples_table_out"> | |
299 <assert_contents> | |
300 <has_n_lines n="5"/> | |
301 <has_n_columns n="8"/> | |
302 <has_line_matching expression="alias\tstatus\taccession\ttitle\tscientific_name\ttaxon_id\tsample_description\tsubmission_date"/> | |
303 </assert_contents> | |
304 </output> | |
305 <output name="runs_table_out"> | |
306 <assert_contents> | |
307 <has_n_lines n="5"/> | |
308 <has_n_columns n="8"/> | |
309 <has_line_matching expression="alias\tstatus\taccession\texperiment_alias\tfile_name\tfile_format\tfile_checksum\tsubmission_date"/> | |
310 <has_line_matching expression="r_(.*)_026\tadd\tena_run_accession\te_(.*)_026\tC026_exp5_clean.fastq.gz\tfastq\tfile_checksum\tsubmission_date_ENA"/> | |
311 </assert_contents> | |
312 </output> | |
313 </test> | |
314 <!--Test build tables from user input fields NON-VIRAL samples--> | |
315 <test> | |
316 <conditional name="action_options"> | |
317 <param name="action" value="add"/> | |
318 <param name="submit_dev" value="False"/> | |
319 <conditional name="input_format_conditional"> | |
320 <param name="input_format" value="build_tables"/> | |
321 <param name="dry_run" value="True"/> | |
322 <conditional name="conditional_viral_metadata"> | |
323 <param name="viral_sample" value="False"/> | |
324 <repeat name="rep_study"> | |
325 <param name="study_title" value="Test study title"/> | |
326 <param name="study_abstract" value="Test study abstract"/> | |
327 <param name="study_type" value="Epigenetics"/> | |
328 <param name="study_pubmed_id" value="Test study pubmedID"/> | |
329 <repeat name="rep_sample"> | |
330 <param name="sample_title" value="Test Sample title"/> | |
331 <param name="sample_description" value="Test Sample description"/> | |
332 <param name="scientific_name" value="Test Sample scientific name"/> | |
333 <param name="tax_id" value="Test Sample tax_id"/> | |
334 <repeat name="rep_experiment"> | |
335 <param name="experiment_title" value="Test experiment title"/> | |
336 <param name="experiment_design" value="Test experiment design description"/> | |
337 <param name="library_strategy" value="CTS"/> | |
338 <param name="library_source" value="GENOMIC"/> | |
339 <param name="library_selection" value="PCR"/> | |
340 <param name="library_layout" value="SINGLE"/> | |
341 <param name="insert_size" value="150"/> | |
342 <param name="library_construction_protocol" value="Test library construction"/> | |
343 <param name="platform" value="ILLUMINA"/> | |
344 <param name="instrument_model" value="Illumina HiSeq 4000"/> | |
345 <repeat name="rep_runs"> | |
346 <param name="upload_files" value="1.fastqsanger.gz,sample.fq" ftype="fastqsanger"/> | |
347 </repeat> | |
348 </repeat> | |
349 </repeat> | |
350 </repeat> | |
351 </conditional> | |
352 </conditional> | |
353 </conditional> | |
354 <param name="center" value="Some research center"/> | |
355 <output name="experiments_table_out"> | |
356 <assert_contents> | |
357 <has_n_lines n="2"/> | |
358 <has_n_columns n="17"/> | |
359 </assert_contents> | |
360 </output> | |
361 <output name="studies_table_out"> | |
362 <assert_contents> | |
363 <has_n_lines n="2"/> | |
364 <has_n_columns n="8"/> | |
365 <has_line_matching expression="alias\tstatus\taccession\ttitle\tstudy_type\tstudy_abstract\tpubmed_id\tsubmission_date"/> | |
366 </assert_contents> | |
367 </output> | |
368 <output name="samples_table_out"> | |
369 <assert_contents> | |
370 <has_n_lines n="2"/> | |
371 <has_n_columns n="8"/> | |
372 </assert_contents> | |
373 </output> | |
374 <output name="runs_table_out"> | |
375 <assert_contents> | |
376 <has_n_lines n="3"/> | |
377 <has_n_columns n="8"/> | |
378 <has_line_matching expression="alias\tstatus\taccession\texperiment_alias\tfile_name\tfile_format\tfile_checksum\tsubmission_date"/> | |
379 </assert_contents> | |
380 </output> | |
381 </test> | |
382 <!--Test RUN failing build tables from user input fields NON-VIRAL samples--> | |
383 <test expect_failure="true"> | |
384 <conditional name="action_options"> | |
385 <param name="action" value="add"/> | |
386 <param name="submit_dev" value="True"/> | |
387 <conditional name="input_format_conditional"> | |
388 <param name="input_format" value="build_tables"/> | |
389 <param name="dry_run" value="False"/> | |
390 <conditional name="conditional_viral_metadata"> | |
391 <param name="viral_sample" value="False"/> | |
392 <repeat name="rep_study"> | |
393 <param name="study_title" value="Test study title"/> | |
394 <param name="study_abstract" value="Test study abstract"/> | |
395 <param name="study_type" value="Epigenetics"/> | |
396 <param name="study_pubmed_id" value="Test study pubmedID"/> | |
397 <repeat name="rep_sample"> | |
398 <param name="sample_title" value="Test Sample title"/> | |
399 <param name="sample_description" value="Test Sample description"/> | |
400 <param name="scientific_name" value="Test Sample scientific name"/> | |
401 <param name="tax_id" value="Test Sample tax_id"/> | |
402 <repeat name="rep_experiment"> | |
403 <param name="experiment_title" value="Test experiment title"/> | |
404 <param name="experiment_design" value="Test experiment design description"/> | |
405 <param name="library_strategy" value="CTS"/> | |
406 <param name="library_source" value="GENOMIC"/> | |
407 <param name="library_selection" value="PCR"/> | |
408 <param name="library_layout" value="SINGLE"/> | |
409 <param name="insert_size" value="150"/> | |
410 <param name="library_construction_protocol" value="Test library construction"/> | |
411 <param name="platform" value="ILLUMINA"/> | |
412 <param name="instrument_model" value="Illumina HiSeq 4000"/> | |
413 <repeat name="rep_runs"> | |
414 <param name="upload_files" value="1.fastqsanger.gz,sample.fq" ftype="fastqsanger"/> | |
415 </repeat> | |
416 </repeat> | |
417 </repeat> | |
418 </repeat> | |
419 </conditional> | |
420 </conditional> | |
421 </conditional> | |
422 <param name="center" value="Some research center"/> | |
423 <assert_stdout> | |
424 <has_text_matching expression="No ENA credentials defined"/> | |
425 </assert_stdout> | |
426 </test> | |
427 <!--Test with submit_test to skip credentials checksRUN failing build tables from user input fields NON-VIRAL samples--> | |
428 <test expect_failure="true"> | |
429 <conditional name="action_options"> | |
430 <param name="action" value="add"/> | |
431 <param name="submit_dev" value="True"/> | |
432 <param name="test_submit" value="True"/> | |
433 <conditional name="input_format_conditional"> | |
434 <param name="input_format" value="build_tables"/> | |
435 <param name="dry_run" value="False"/> | |
436 <conditional name="conditional_viral_metadata"> | |
437 <param name="viral_sample" value="False"/> | |
438 <repeat name="rep_study"> | |
439 <param name="study_title" value="Test study title"/> | |
440 <param name="study_abstract" value="Test study abstract"/> | |
441 <param name="study_type" value="Epigenetics"/> | |
442 <param name="study_pubmed_id" value="Test study pubmedID"/> | |
443 <repeat name="rep_sample"> | |
444 <param name="sample_title" value="Test Sample title"/> | |
445 <param name="sample_description" value="Test Sample description"/> | |
446 <param name="scientific_name" value="Test Sample scientific name"/> | |
447 <param name="tax_id" value="Test Sample tax_id"/> | |
448 <repeat name="rep_experiment"> | |
449 <param name="experiment_title" value="Test experiment title"/> | |
450 <param name="experiment_design" value="Test experiment design description"/> | |
451 <param name="library_strategy" value="CTS"/> | |
452 <param name="library_source" value="GENOMIC"/> | |
453 <param name="library_selection" value="PCR"/> | |
454 <param name="library_layout" value="SINGLE"/> | |
455 <param name="insert_size" value="150"/> | |
456 <param name="library_construction_protocol" value="Test library construction"/> | |
457 <param name="platform" value="ILLUMINA"/> | |
458 <param name="instrument_model" value="Illumina HiSeq 4000"/> | |
459 <repeat name="rep_runs"> | |
460 <param name="upload_files" value="1.fastqsanger.gz,sample.fq" ftype="fastqsanger"/> | |
461 </repeat> | |
462 </repeat> | |
463 </repeat> | |
464 </repeat> | |
465 </conditional> | |
466 </conditional> | |
467 </conditional> | |
468 <param name="center" value="Some research center"/> | |
469 <assert_command> | |
470 <has_text_matching expression="ena-upload-cli"/> | |
471 <has_text_matching expression="--data '1.fastqsanger.gz' 'sample.fq'"/> | |
472 <has_text_matching expression="--action 'add' --center 'Some research center'"/> | |
473 </assert_command> | |
474 <assert_stderr> | |
475 <has_text_matching expression="ENA_upload: error: Oops, the file test_fake_path does not exist"/> | |
476 </assert_stderr> | |
477 </test> | |
478 <!--test viral submission - User input metadata--> | |
479 <test expect_failure="true"> | |
480 <conditional name="action_options"> | |
481 <param name="action" value="add"/> | |
482 <param name="submit_dev" value="False"/> | |
483 <param name="test_submit" value="True"/> | |
484 <conditional name="input_format_conditional"> | |
485 <param name="input_format" value="build_tables"/> | |
486 <param name="dry_run" value="False"/> | |
487 <conditional name="conditional_viral_metadata"> | |
488 <param name="viral_sample" value="True"/> | |
489 <repeat name="rep_study"> | |
490 <param name="study_title" value="Test study title"/> | |
491 <param name="study_abstract" value="Test study abstract"/> | |
492 <param name="study_type" value="Epigenetics"/> | |
493 <param name="study_pubmed_id" value="Test study pubmedID"/> | |
494 <repeat name="rep_sample"> | |
495 <param name="sample_title" value="Test Sample title"/> | |
496 <param name="sample_description" value="Test Sample description"/> | |
497 <param name="scientific_name" value="Test Sample scientific name"/> | |
498 <param name="tax_id" value="Test Sample tax_id"/> | |
499 <param name="collection_date" value="2020"/> | |
500 <param name="geo_location_country" value="Belgium"/> | |
501 <param name="host_common_name" value="Human"/> | |
502 <param name="host_subject_id" value="Patient_001"/> | |
503 <param name="host_health_state" value="healthy"/> | |
504 <param name="host_sex" value="female"/> | |
505 <param name="host_scientific_name" value="homo sapiens"/> | |
506 <param name="collector_name" value="John The Collector"/> | |
507 <param name="collecting_institution" value="Hospital 01"/> | |
508 <param name="isolate" value="sample_001"/> | |
509 <repeat name="rep_experiment"> | |
510 <param name="experiment_title" value="Test experiment title"/> | |
511 <param name="experiment_design" value="Test experiment design description"/> | |
512 <param name="library_strategy" value="CTS"/> | |
513 <param name="library_source" value="GENOMIC"/> | |
514 <param name="library_selection" value="PCR"/> | |
515 <param name="library_layout" value="SINGLE"/> | |
516 <param name="insert_size" value="150"/> | |
517 <param name="library_construction_protocol" value="Test library construction"/> | |
518 <param name="platform" value="ILLUMINA"/> | |
519 <param name="instrument_model" value="Illumina HiSeq 4000"/> | |
520 <repeat name="rep_runs"> | |
521 <param name="upload_files" value="1.fastqsanger.gz,sample.fq" ftype="fastqsanger"/> | |
522 </repeat> | |
523 </repeat> | |
524 </repeat> | |
525 </repeat> | |
526 </conditional> | |
527 </conditional> | |
528 </conditional> | |
529 <param name="center" value="Some research center"/> | |
530 <assert_command> | |
531 <has_text_matching expression="ena-upload-cli"/> | |
532 <has_text_matching expression="--data '1.fastqsanger.gz' 'sample.fq'"/> | |
533 <has_text_matching expression="--action 'add' --center 'Some research center'"/> | |
534 <has_text_matching expression="--vir"/> | |
535 </assert_command> | |
536 <assert_stderr> | |
537 <has_text_matching expression="ENA_upload: error: Oops, the file test_fake_path does not exist"/> | |
538 </assert_stderr> | |
539 </test> | |
540 </tests> | |
541 <help><![CDATA[ | |
542 This is a wrapper for the ENA upload tool in https://github.com/usegalaxy-eu/ena-upload-cli | |
543 The input metadata can be submitted following the tabular format of the templates in https://github.com/usegalaxy-eu/ena-upload-cli/tree/master/example_tables | |
544 It is also possible to submit an excel file by following the template in https://drive.google.com/file/d/1ncC22--tW2v-EI-te_r86sAZujIPAjlX/view?usp=sharing | |
545 For viral submissions a larger set of metadata is required, you can find the template in https://drive.google.com/file/d/1U4VdcczsIecIXxseV8svE1zO_CBUadog/view?usp=sharing | |
546 ]]></help> | |
547 <citations> | |
548 </citations> | |
549 </tool> |