# HG changeset patch # User iuc # Date 1727458900 0 # Node ID 959a14c1f2dddb782d4727f6b1cf6ed6054ce222 # Parent babbcf02d35c4684e2e256f3d8a956006381ad88 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/falco commit a71120623d1dc818a107ca32f2c232fd47d819ea diff -r babbcf02d35c -r 959a14c1f2dd falco.xml --- a/falco.xml Tue Sep 10 19:02:42 2024 +0000 +++ b/falco.xml Fri Sep 27 17:41:40 2024 +0000 @@ -1,7 +1,7 @@ An alternative, more performant implementation of FastQC for high throughput sequence quality control - 1.2.3 + 1.2.4 0 @@ -74,28 +74,81 @@ + - - + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + - - + + + + + + - - + + + + + + - + - - + + + + + + + - + - - - - - - - + + + + + + - - + + + + + + - - + + + + + + - + - - - - + + + + + + >Basic Statistics pass #Measure Value Filename 1000trimmed_fastq @@ -1597,114 +1597,114 @@ #Sequence Count Percentage Possible Source ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672783 No Hit >>END_MODULE ->>Adapter Content warn +>>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG 1 0 0 0 0 0.0203874 0 -2 0 0 0 0 0.0815494 0 -3 0 0 0 0 0.142712 0 -4 0 0 0 0 0.183486 0 -5 0 0 0 0 0.285423 0 -6 0 0 0 0 0.38736 0 -7 0 0 0 0 0.489297 0 -8 0 0 0 0 0.591233 0 -9 0 0 0 0 0.672783 0 -10 0 0 0 0 0.754332 0 -11 0 0 0 0 0.835882 0 -12 0 0 0 0 0.917431 0 -13 0 0 0 0 1.01937 0 -14 0 0 0 0 1.1213 0 -15 0 0 0 0 1.24363 0 -16 0 0 0 0 1.34557 0 -17 0 0 0 0 1.46789 0 -18 0 0 0 0 1.59021 0 -19 0 0 0 0 1.67176 0 -20 0.122324 0 0 0 1.75331 0 -21 0.122324 0 0 0 1.83486 0 -22 0.122324 0 0 0 1.89602 0 -23 0.122324 0 0 0 1.95719 0 -24 0.122324 0 0 0 2.01835 0 -25 0.122324 0 0 0 2.07951 0 -26 0.122324 0 0 0 2.14067 0 -27 0.142712 0 0 0 2.20183 0 -28 0.183486 0 0 0 2.263 0 -29 0.224261 0 0 0 2.32416 0 -30 0.224261 0 0 0 2.38532 0 -31 0.224261 0 0 0 2.44648 0 -32 0.224261 0 0 0 2.50765 0 -33 0.224261 0 0 0 2.60958 0 -34 0.224261 0 0 0 2.71152 0 -35 0.265036 0 0 0 2.81346 0 -36 0.285423 0 0 0 2.89501 0 -37 0.326198 0 0 0 2.99694 0 -38 0.407747 0 0 0 3.09888 0 -39 0.468909 0 0 0 3.20082 0 -40 0.468909 0 0 0 3.30275 0 -41 0.468909 0 0 0 3.40469 0 -42 0.468909 0 0 0 3.48624 0 -43 0.468909 0 0 0 3.56779 0 -44 0.468909 0 0 0 3.60856 0 -45 0.468909 0 0 0 3.62895 0 -46 0.468909 0 0 0 3.64934 0 -47 0.468909 0 0 0 3.66972 0 -48 0.468909 0 0 0 3.69011 0 -49 0.468909 0 0 0 3.7105 0 -50 0.468909 0 0 0 3.7105 0 -51 0.468909 0 0 0 3.7105 0 -52 0.468909 0 0 0 3.7105 0 -53 0.468909 0 0 0 3.7105 0 -54 0.468909 0 0 0 3.7105 0 -55 0.468909 0 0 0 3.7105 0 -56 0.468909 0 0 0 3.7105 0 -57 0.468909 0 0 0 3.7105 0 -58 0.468909 0 0 0 3.7105 0 -59 0.468909 0 0 0 3.73089 0 -60 0.468909 0 0 0 3.75127 0 -61 0.468909 0 0 0 3.77166 0 -62 0.468909 0 0 0 3.81244 0 -63 0.468909 0 0 0 3.85321 0 -64 0.468909 0 0 0 3.89399 0 -65 0.468909 0 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0.468909 0 0 0 0.326198 0 +98 0.468909 0 0 0 0.326198 0 +99 0.468909 0 0 0 0.326198 0 +100 0.468909 0 0 0 0.326198 0 +101 0.468909 0 0 0 0.326198 0 +102 0.468909 0 0 0 0.326198 0 +103 0.468909 0 0 0 0.326198 0 +104 0.468909 0 0 0 0.326198 0 +105 0.468909 0 0 0 0.326198 0 +106 0.468909 0 0 0 0.326198 0 +107 0.468909 0 0 0 0.326198 0 +108 0.468909 0 0 0 0.326198 0 >>END_MODULE diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_data_adapters.txt --- a/test-data/fastqc_data_adapters.txt Tue Sep 10 19:02:42 2024 +0000 +++ b/test-data/fastqc_data_adapters.txt Fri Sep 27 17:41:40 2024 +0000 @@ -1,4 +1,4 @@ -##Falco 1.2.3 +##Falco 1.2.4 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq @@ -1672,39 +1672,39 @@ 71 0.468909 0 0 0 0 72 0.468909 0 0 0 0 73 0.468909 0 0 0 0 -74 0.489297 0 0 0 0 -75 0.489297 0 0 0 0 -76 0.489297 0 0 0 0 -77 0.489297 0 0 0 0 -78 0.489297 0 0 0 0 -79 0.489297 0 0 0 0 -80 0.489297 0 0 0 0 -81 0.489297 0 0 0 0 -82 0.489297 0 0 0 0 -83 0.509684 0 0 0 0 -84 0.509684 0 0 0 0 -85 0.509684 0 0 0 0 -86 0.509684 0 0 0 0 -87 0.509684 0 0 0 0 -88 0.509684 0 0 0 0 -89 0.509684 0 0 0 0 -90 0.509684 0 0 0 0 -91 0.509684 0 0 0 0 -92 0.570846 0 0 0 0 -93 0.632008 0 0 0 0 -94 0.632008 0 0 0 0 -95 0.632008 0 0 0 0 -96 0.632008 0 0 0 0 -97 0.632008 0 0 0 0 -98 0.632008 0 0 0 0 -99 0.632008 0 0 0 0 -100 0.632008 0 0 0 0 -101 0.632008 0 0 0 0 -102 0.632008 0 0 0 0 -103 0.632008 0 0 0 0 -104 0.632008 0 0 0 0 -105 0.632008 0 0 0 0 -106 0.632008 0 0 0 0 -107 0.632008 0 0 0 0 -108 0.632008 0 0 0 0 +74 0.468909 0 0 0 0 +75 0.468909 0 0 0 0 +76 0.468909 0 0 0 0 +77 0.468909 0 0 0 0 +78 0.468909 0 0 0 0 +79 0.468909 0 0 0 0 +80 0.468909 0 0 0 0 +81 0.468909 0 0 0 0 +82 0.468909 0 0 0 0 +83 0.468909 0 0 0 0 +84 0.468909 0 0 0 0 +85 0.468909 0 0 0 0 +86 0.468909 0 0 0 0 +87 0.468909 0 0 0 0 +88 0.468909 0 0 0 0 +89 0.468909 0 0 0 0 +90 0.468909 0 0 0 0 +91 0.468909 0 0 0 0 +92 0.468909 0 0 0 0 +93 0.468909 0 0 0 0 +94 0.468909 0 0 0 0 +95 0.468909 0 0 0 0 +96 0.468909 0 0 0 0 +97 0.468909 0 0 0 0 +98 0.468909 0 0 0 0 +99 0.468909 0 0 0 0 +100 0.468909 0 0 0 0 +101 0.468909 0 0 0 0 +102 0.468909 0 0 0 0 +103 0.468909 0 0 0 0 +104 0.468909 0 0 0 0 +105 0.468909 0 0 0 0 +106 0.468909 0 0 0 0 +107 0.468909 0 0 0 0 +108 0.468909 0 0 0 0 >>END_MODULE diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_data_adapters_summary.txt --- a/test-data/fastqc_data_adapters_summary.txt Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,11 +0,0 @@ -PASS Basic Statistics 1000trimmed_fastq -PASS Per base sequence quality 1000trimmed_fastq -FAIL Per tile sequence quality 1000trimmed_fastq -PASS Per sequence quality scores 1000trimmed_fastq -FAIL Per base sequence content 1000trimmed_fastq -WARN Per sequence GC content 1000trimmed_fastq -PASS Per base N content 1000trimmed_fastq -WARN Sequence Length Distribution 1000trimmed_fastq -PASS Sequence Duplication Levels 1000trimmed_fastq -WARN Overrepresented sequences 1000trimmed_fastq -PASS Adapter Content 1000trimmed_fastq diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_data_contaminant_summary.txt --- a/test-data/fastqc_data_contaminant_summary.txt Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,11 +0,0 @@ -PASS Basic Statistics 1000trimmed_fastq -PASS Per base sequence quality 1000trimmed_fastq -FAIL Per tile sequence quality 1000trimmed_fastq -PASS Per sequence quality scores 1000trimmed_fastq -FAIL Per base sequence content 1000trimmed_fastq -WARN Per sequence GC content 1000trimmed_fastq -PASS Per base N content 1000trimmed_fastq -WARN Sequence Length Distribution 1000trimmed_fastq -PASS Sequence Duplication Levels 1000trimmed_fastq -WARN Overrepresented sequences 1000trimmed_fastq -PASS Adapter Content 1000trimmed_fastq diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_data_contaminants.txt --- a/test-data/fastqc_data_contaminants.txt Tue Sep 10 19:02:42 2024 +0000 +++ b/test-data/fastqc_data_contaminants.txt Fri Sep 27 17:41:40 2024 +0000 @@ -1,4 +1,4 @@ -##Falco 1.2.3 +##Falco 1.2.4 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq @@ -1595,116 +1595,116 @@ >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source -ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672783 No Hit +ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672783 Test sequence >>END_MODULE ->>Adapter Content warn +>>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG 1 0 0 0 0 0.0203874 0 -2 0 0 0 0 0.0815494 0 -3 0 0 0 0 0.142712 0 -4 0 0 0 0 0.183486 0 -5 0 0 0 0 0.285423 0 -6 0 0 0 0 0.38736 0 -7 0 0 0 0 0.489297 0 -8 0 0 0 0 0.591233 0 -9 0 0 0 0 0.672783 0 -10 0 0 0 0 0.754332 0 -11 0 0 0 0 0.835882 0 -12 0 0 0 0 0.917431 0 -13 0 0 0 0 1.01937 0 -14 0 0 0 0 1.1213 0 -15 0 0 0 0 1.24363 0 -16 0 0 0 0 1.34557 0 -17 0 0 0 0 1.46789 0 -18 0 0 0 0 1.59021 0 -19 0 0 0 0 1.67176 0 -20 0.122324 0 0 0 1.75331 0 -21 0.122324 0 0 0 1.83486 0 -22 0.122324 0 0 0 1.89602 0 -23 0.122324 0 0 0 1.95719 0 -24 0.122324 0 0 0 2.01835 0 -25 0.122324 0 0 0 2.07951 0 -26 0.122324 0 0 0 2.14067 0 -27 0.142712 0 0 0 2.20183 0 -28 0.183486 0 0 0 2.263 0 -29 0.224261 0 0 0 2.32416 0 -30 0.224261 0 0 0 2.38532 0 -31 0.224261 0 0 0 2.44648 0 -32 0.224261 0 0 0 2.50765 0 -33 0.224261 0 0 0 2.60958 0 -34 0.224261 0 0 0 2.71152 0 -35 0.265036 0 0 0 2.81346 0 -36 0.285423 0 0 0 2.89501 0 -37 0.326198 0 0 0 2.99694 0 -38 0.407747 0 0 0 3.09888 0 -39 0.468909 0 0 0 3.20082 0 -40 0.468909 0 0 0 3.30275 0 -41 0.468909 0 0 0 3.40469 0 -42 0.468909 0 0 0 3.48624 0 -43 0.468909 0 0 0 3.56779 0 -44 0.468909 0 0 0 3.60856 0 -45 0.468909 0 0 0 3.62895 0 -46 0.468909 0 0 0 3.64934 0 -47 0.468909 0 0 0 3.66972 0 -48 0.468909 0 0 0 3.69011 0 -49 0.468909 0 0 0 3.7105 0 -50 0.468909 0 0 0 3.7105 0 -51 0.468909 0 0 0 3.7105 0 -52 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0 -87 0.509684 0 0 0 4.91335 0 -88 0.509684 0 0 0 4.9949 0 -89 0.509684 0 0 0 5.05607 0 -90 0.509684 0 0 0 5.09684 0 -91 0.509684 0 0 0 5.158 0 -92 0.570846 0 0 0 5.21916 0 -93 0.632008 0 0 0 5.28033 0 -94 0.632008 0 0 0 5.34149 0 -95 0.632008 0 0 0 5.40265 0 -96 0.632008 0 0 0 5.46381 0 -97 0.632008 0 0 0 5.52497 0 -98 0.632008 0 0 0 5.52497 0 -99 0.632008 0 0 0 5.52497 0 -100 0.632008 0 0 0 5.52497 0 -101 0.632008 0 0 0 5.52497 0 -102 0.632008 0 0 0 5.52497 0 -103 0.632008 0 0 0 5.52497 0 -104 0.632008 0 0 0 5.52497 0 -105 0.632008 0 0 0 5.52497 0 -106 0.632008 0 0 0 5.52497 0 -107 0.632008 0 0 0 5.52497 0 -108 0.632008 0 0 0 5.52497 0 +2 0 0 0 0 0.0611621 0 +3 0 0 0 0 0.0611621 0 +4 0 0 0 0 0.0611621 0 +5 0 0 0 0 0.122324 0 +6 0 0 0 0 0.122324 0 +7 0 0 0 0 0.122324 0 +8 0 0 0 0 0.142712 0 +9 0 0 0 0 0.142712 0 +10 0 0 0 0 0.142712 0 +11 0 0 0 0 0.142712 0 +12 0 0 0 0 0.142712 0 +13 0 0 0 0 0.163099 0 +14 0 0 0 0 0.163099 0 +15 0 0 0 0 0.183486 0 +16 0 0 0 0 0.203874 0 +17 0 0 0 0 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3.0186 -9 1 -0.439144 -9 2 -0.772074 -9 3 0.254047 -9 4 0.48607 -9 5 0.546022 -9 6 0.0722848 -9 7 -1.61776 -9 8 -0.688039 -9 9 -0.228381 -9 10 -0.0434537 -9 11 0.0485763 -9 12 0.699247 -9 13 0.562668 -9 14 -0.641509 -9 15 0.0385433 -9 16 0.851939 -9 17 0.573057 -9 18 -0.203506 -9 19 -0.0390141 -9 20 -0.24492 -9 21 -0.539138 -9 22 0.332942 -9 23 -0.297086 -9 24 0.26364 -9 25 0.301358 -9 26 0.342857 -9 27 0.527139 -9 28 0.0751259 -9 29 0.223292 -9 30 0.194619 -9 31 0.279018 -9 32 0.000342309 -9 33 1.15811 -9 34 0.738165 -9 35 0.432461 -9 36 0.319052 -9 37 -1.09152 -9 38 -0.152093 -9 39 0.253402 -9 40 0.366605 -9 41 0.734721 -9 42 0.803621 -9 43 -0.143133 -9 44 1.27048 -9 45 0.751179 -9 46 0.213592 -9 47 4.09189 -9 48 2.42396 -9 49 -0.209497 -9 50 -1.27425 -9 51 1.59959 -9 52 3.38543 -9 53 1.66473 -9 54 0.909091 -9 55 -0.416058 -9 56 0.340411 -9 57 0.908427 -9 58 0.88852 -9 59 1.45786 -9 60 0.854015 -9 61 -0.670869 -9 62 -0.0106171 -9 63 -1.50564 -9 64 0.928998 -9 65 -0.00467914 -9 66 1.08957 -9 67 0.304813 -9 68 1.3369 -9 69 0.65107 -9 70 0.85361 -9 71 0.442513 -9 72 0.143048 -9 73 1.1504 -9 74 2.3643 -9 75 -0.25 -9 76 1.64305 -9 77 -0.165107 -9 78 1.12968 -9 79 0.770722 -9 80 1.40441 -9 81 2.01337 -9 82 1.07219 -9 83 2.625 -9 84 1.92781 -9 85 -0.33623 -9 86 0.339572 -9 87 2.08356 -9 88 2.54746 -9 89 3.73061 -9 90 0.555481 -9 91 -0.921791 -9 92 0.139037 -9 93 1.20187 -9 94 1.74666 -9 95 1.62901 -9 96 1.4893 -9 97 0.989305 -9 98 1.61564 -9 99 0.551471 -9 100 0.595588 -9 101 3.61898 -9 102 2.05949 -9 103 0.502674 -9 104 0.139706 -9 105 2.16778 -9 106 3.03209 -9 107 1.50204 -9 108 1.1686 -10 1 -1.10635 -10 2 -0.392764 -10 3 -0.996955 -10 4 -0.348905 -10 5 0.144938 -10 6 0.838319 -10 7 0.0476026 -10 8 1.00367 -10 9 0.63544 -10 10 -2.21012 -10 11 -0.334757 -10 12 0.330657 -10 13 0.858721 -10 14 -0.183176 -10 15 0.239026 -10 16 1.07828 -10 17 1.55701 -10 18 1.36652 -10 19 1.13466 -10 20 0.158442 -10 21 -0.872471 -10 22 0.51449 -10 23 0.752022 -10 24 1.53894 -10 25 1.22695 -10 26 0.942857 -10 27 0.735911 -10 28 0.629512 -10 29 0.079916 -10 30 2.65015 -10 31 1.57977 -10 32 2.01225 -10 33 -0.0641166 -10 34 1.10854 -10 35 0.251466 -10 36 1.83621 -10 37 1.21241 -10 38 0.0704133 -10 39 -0.966547 -10 40 2.06226 -10 41 -0.97004 -10 42 -1.01543 -10 43 -0.0597997 -10 44 0.465051 -10 45 -0.330552 -10 46 -0.119741 -10 47 -1.08668 -10 48 1.80059 -10 49 2.38141 -10 50 2.25432 -10 51 -1.09272 -10 52 -1.81659 -10 53 1.69504 -10 54 1.44444 -10 55 -2.08273 -10 56 -1.16464 -10 57 -1.96026 -10 58 1.6764 -10 59 0.0235199 -10 60 -0.0348743 -10 61 -0.711273 -10 62 -0.0308191 -10 63 0.282238 -10 64 -5.0811 -10 65 -0.984477 -10 66 0.321895 -10 67 -0.169935 -10 68 -1.00654 -10 69 0.570261 -10 70 -0.0857843 -10 71 -0.102941 -10 72 1.55719 -10 73 1.78676 -10 74 1.72794 -10 75 -1.25 -10 76 1.05719 -10 77 0.754085 -10 78 1.66503 -10 79 -1.23938 -10 80 -0.484477 -10 81 0.558824 -10 82 -0.604575 -10 83 0.291667 -10 84 1.60458 -10 85 1.62337 -10 86 1.46078 -10 87 -1.89624 -10 88 -0.816176 -10 89 -1.13807 -10 90 0.363562 -10 91 1.05801 -10 92 0.189542 -10 93 -0.71732 -10 94 2.33252 -10 95 -3.34069 -10 96 -1.5915 -10 97 0.352941 -10 98 1.76716 -10 99 -1.22631 -10 100 -0.515523 -10 101 -1.59314 -10 102 1.67565 -10 103 3.30065 -10 104 1.91748 -10 105 -1.28676 -10 106 -2.16993 -10 107 1.53234 -10 108 1.7686 ->>END_MODULE ->>Per sequence quality scores pass -#Quality Count -20 7 -21 24 -22 47 -23 78 -24 226 -25 513 -26 830 -27 1017 -28 947 -29 645 -30 352 -31 157 -32 55 -33 6 -34 1 ->>END_MODULE ->>Per base sequence content fail -#Base G A T C -1 21.2232 33.0071 25.9735 19.7961 -2 23.7658 31.4157 27.3154 17.5031 -3 21.8769 28.7058 29.4623 19.955 -4 21.5505 28.2953 28.8505 21.3037 -5 21.2191 30.2059 28.2713 20.3037 -6 22.1243 28.9463 26.3644 22.5651 -7 21.944 29.966 28.3956 19.6944 -8 21.3781 28.8252 27.8622 21.9345 -9 20.078 29.0882 28.655 22.1789 -10-11 21.2432 29.4689 27.6802 21.6076 -12-13 20.8277 29.037 29.1836 20.9517 -14-15 20.24 28.9753 29.4715 21.3132 -16-17 19.8387 29.3964 29.6217 21.1431 -18-19 20.8456 27.8715 29.9731 21.3099 -20-21 20.5382 28.9737 29.1114 21.3767 -22-23 20.8808 30.1295 29.3005 19.6891 -24-25 20.507 29.0773 29.0504 21.3653 -26-27 20.866 28.0549 30.3391 20.7399 -28-29 19.326 30.8425 28.7766 21.0549 -30-31 20.6388 29.1923 29.4994 20.6695 -32-33 19.4799 29.0099 29.3814 22.1289 -34-35 19.6674 29.7132 30.2053 20.4141 -36-37 20.4435 29.854 30.6111 19.0914 -38-39 20.9927 28.0417 30.0888 20.8768 -40-41 19.8602 28.6801 30.3248 21.1349 -42-43 20.0132 27.8732 30.8234 21.2902 -44-45 18.9414 30.1923 29.9549 20.9115 -46-47 20.1889 28.1266 31.4701 20.2144 -48-49 19.5616 29.6892 28.7736 21.9756 -50-51 20.5066 28.2576 30.7598 20.476 -52-53 17.6913 29.918 31.8648 20.526 -54-55 17.2149 32.3099 29.5322 20.943 -56-57 19.3086 30.8937 30.8937 18.904 -58-59 20.0147 29.7277 31.4202 18.8374 -60-61 17.4025 33.1862 29.507 19.9043 -62-63 18.543 29.7277 32.2664 19.4628 -64-65 16.8936 32.9407 30.1803 19.9853 -66-67 18.5199 28.9028 32.8056 19.7717 -68-69 17.268 30.3756 31.6642 20.6922 -70-71 17.673 27.7982 35.3461 19.1826 -72-73 16.2003 29.5287 33.8733 20.3976 -74-75 18.3726 28.0191 34.5361 19.0722 -76-77 16.6789 31.701 31.7378 19.8822 -78-79 18.7776 28.3873 32.6215 20.2135 -80-81 16.3844 30.9278 32.9161 19.7717 -82-83 17.0839 30.9278 32.5479 19.4404 -84-85 16.9367 31.5906 31.4433 20.0295 -86-87 17.489 27.9087 34.2047 20.3976 -88-89 16.7894 32.3638 31.0751 19.7717 -90-91 19.5876 29.7496 33.542 17.1208 -92-93 17.7467 30.0442 31.3328 20.8763 -94-95 18.9249 28.461 33.3947 19.2194 -96-97 16.4212 29.4183 32.4742 21.6863 -98-99 18.1581 29.3438 33.1469 19.3512 -100-101 17.7736 29.8491 33.283 19.0943 -102-103 18.3396 29.5094 30.9057 21.2453 -104-105 18.0377 28.6792 31.7736 21.5094 -106-107 17.2783 29.3578 33.5245 19.8394 -108 20.0535 26.3815 31.7291 21.836 ->>END_MODULE ->>Per sequence GC content warn -#GC Content Count -0 15 -1 15.5 -2 16.5 -3 17 -4 18 -5 21.5 -6 26.5 -7 30 -8 33.5 -9 36 -10 41 -11 47 -12 47.5 -13 56 -14 65.5 -15 69 -16 72.5 -17 77.5 -18 85.5 -19 94.5 -20 105.5 -21 113 -22 120 -23 131.5 -24 150 -25 172.5 -26 198 -27 217.5 -28 244.5 -29 281.5 -30 314.5 -31 337 -32 365 -33 402.5 -34 436 -35 463 -36 481.5 -37 505 -38 525 -39 510.5 -40 490.5 -41 493 -42 487 -43 483.5 -44 488 -45 475.5 -46 468 -47 468.5 -48 477 -49 473 -50 437.5 -51 416 -52 405.5 -53 397 -54 386 -55 365 -56 346 -57 343 -58 334 -59 320 -60 319 -61 301.5 -62 276.5 -63 245.5 -64 207.5 -65 191 -66 182 -67 173 -68 167 -69 151.5 -70 131.5 -71 121 -72 117.5 -73 110.5 -74 104 -75 90.5 -76 75 -77 67.5 -78 62.5 -79 61.5 -80 59 -81 57 -82 55 -83 47 -84 39 -85 38 -86 36.5 -87 35.5 -88 28.5 -89 21 -90 19 -91 17 -92 15.5 -93 14.5 -94 14 -95 13.5 -96 14.5 -97 15.5 -98 15.5 -99 16 -100 15 ->>END_MODULE ->>Per base N content pass -#Base N-Count -1 0 -2 0 -3 0 -4 0 -5 0 -6 0 -7 0 -8 0 -9 0 -10-11 0 -12-13 0 -14-15 0 -16-17 0 -18-19 0 -20-21 0 -22-23 0 -24-25 0 -26-27 0 -28-29 0 -30-31 0 -32-33 0 -34-35 0 -36-37 0 -38-39 0 -40-41 0 -42-43 0 -44-45 0 -46-47 0 -48-49 0 -50-51 0 -52-53 0 -54-55 0 -56-57 0 -58-59 0 -60-61 0 -62-63 0 -64-65 0 -66-67 0 -68-69 0 -70-71 0 -72-73 0 -74-75 0 -76-77 0 -78-79 0 -80-81 0 -82-83 0 -84-85 0 -86-87 0 -88-89 0 -90-91 0 -92-93 0 -94-95 0 -96-97 0 -98-99 0 -100-101 0 -102-103 0 -104-105 0 -106-107 0 -108 0 ->>END_MODULE ->>Sequence Length Distribution warn -#Length Count -1 3.0 -2 11.0 -3 28.0 -4 56.0 -5 43.0 -6 52.0 -7 39.0 -8 56.0 -9 60.0 -10 57.0 -11 43.0 -12 46.0 -13 45.0 -14 66.0 -15 59.0 -16 49.0 -17 73.0 -18 54.0 -19 44.0 -20 52.0 -21 73.0 -22 72.0 -23 68.0 -24 56.0 -25 86.0 -26 92.0 -27 75.0 -28 69.0 -29 74.0 -30 96.0 -31 72.0 -32 81.0 -33 65.0 -34 87.0 -35 86.0 -36 87.0 -37 100.0 -38 82.0 -39 78.0 -40 76.0 -41 79.0 -42 88.0 -43 83.0 -44 75.0 -45 74.0 -46 72.0 -47 84.0 -48 74.0 -49 81.0 -50 91.0 -51 80.0 -52 98.0 -53 43.0 -54 8.0 -55 4.0 -56 1.0 -64 1.0 -97 1.0 -98 32.0 -106 34.0 -107 169.0 -108 1122.0 ->>END_MODULE ->>Sequence Duplication Levels pass -#Total Deduplicated Percentage 98.736 -#Duplication Level Percentage of deduplicated Percentage of total -1 99.4425 98.1855 -2 0.474912 0.937819 -3 0.0412967 0.122324 -4 0.0206484 0.0815494 -5 0 0 -6 0 0 -7 0 0 -8 0 0 -9 0 0 ->10 0.0206484 0.672783 ->50 0 0 ->100 0 0 ->500 0 0 ->1k 0 0 ->5k 0 0 ->10k+ 0 0 ->>END_MODULE ->>Overrepresented sequences warn -#Sequence Count Percentage Possible Source -ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672783 No Hit ->>END_MODULE ->>Adapter Content warn -#Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG -1 0 0 0 0 0.0203874 0 -2 0 0 0 0 0.0815494 0 -3 0 0 0 0 0.142712 0 -4 0 0 0 0 0.183486 0 -5 0 0 0 0 0.285423 0 -6 0 0 0 0 0.38736 0 -7 0 0 0 0 0.489297 0 -8 0 0 0 0 0.591233 0 -9 0 0 0 0 0.672783 0 -10 0 0 0 0 0.754332 0 -11 0 0 0 0 0.835882 0 -12 0 0 0 0 0.917431 0 -13 0 0 0 0 1.01937 0 -14 0 0 0 0 1.1213 0 -15 0 0 0 0 1.24363 0 -16 0 0 0 0 1.34557 0 -17 0 0 0 0 1.46789 0 -18 0 0 0 0 1.59021 0 -19 0 0 0 0 1.67176 0 -20 0.122324 0 0 0 1.75331 0 -21 0.122324 0 0 0 1.83486 0 -22 0.122324 0 0 0 1.89602 0 -23 0.122324 0 0 0 1.95719 0 -24 0.122324 0 0 0 2.01835 0 -25 0.122324 0 0 0 2.07951 0 -26 0.122324 0 0 0 2.14067 0 -27 0.142712 0 0 0 2.20183 0 -28 0.183486 0 0 0 2.263 0 -29 0.224261 0 0 0 2.32416 0 -30 0.224261 0 0 0 2.38532 0 -31 0.224261 0 0 0 2.44648 0 -32 0.224261 0 0 0 2.50765 0 -33 0.224261 0 0 0 2.60958 0 -34 0.224261 0 0 0 2.71152 0 -35 0.265036 0 0 0 2.81346 0 -36 0.285423 0 0 0 2.89501 0 -37 0.326198 0 0 0 2.99694 0 -38 0.407747 0 0 0 3.09888 0 -39 0.468909 0 0 0 3.20082 0 -40 0.468909 0 0 0 3.30275 0 -41 0.468909 0 0 0 3.40469 0 -42 0.468909 0 0 0 3.48624 0 -43 0.468909 0 0 0 3.56779 0 -44 0.468909 0 0 0 3.60856 0 -45 0.468909 0 0 0 3.62895 0 -46 0.468909 0 0 0 3.64934 0 -47 0.468909 0 0 0 3.66972 0 -48 0.468909 0 0 0 3.69011 0 -49 0.468909 0 0 0 3.7105 0 -50 0.468909 0 0 0 3.7105 0 -51 0.468909 0 0 0 3.7105 0 -52 0.468909 0 0 0 3.7105 0 -53 0.468909 0 0 0 3.7105 0 -54 0.468909 0 0 0 3.7105 0 -55 0.468909 0 0 0 3.7105 0 -56 0.468909 0 0 0 3.7105 0 -57 0.468909 0 0 0 3.7105 0 -58 0.468909 0 0 0 3.7105 0 -59 0.468909 0 0 0 3.73089 0 -60 0.468909 0 0 0 3.75127 0 -61 0.468909 0 0 0 3.77166 0 -62 0.468909 0 0 0 3.81244 0 -63 0.468909 0 0 0 3.85321 0 -64 0.468909 0 0 0 3.89399 0 -65 0.468909 0 0 0 3.93476 0 -66 0.468909 0 0 0 3.97554 0 -67 0.468909 0 0 0 4.01631 0 -68 0.468909 0 0 0 4.05708 0 -69 0.468909 0 0 0 4.09786 0 -70 0.468909 0 0 0 4.13863 0 -71 0.468909 0 0 0 4.17941 0 -72 0.468909 0 0 0 4.22018 0 -73 0.468909 0 0 0 4.26096 0 -74 0.489297 0 0 0 4.32212 0 -75 0.489297 0 0 0 4.38328 0 -76 0.489297 0 0 0 4.42406 0 -77 0.489297 0 0 0 4.46483 0 -78 0.489297 0 0 0 4.50561 0 -79 0.489297 0 0 0 4.54638 0 -80 0.489297 0 0 0 4.58716 0 -81 0.489297 0 0 0 4.62793 0 -82 0.489297 0 0 0 4.66871 0 -83 0.509684 0 0 0 4.70948 0 -84 0.509684 0 0 0 4.75025 0 -85 0.509684 0 0 0 4.79103 0 -86 0.509684 0 0 0 4.8318 0 -87 0.509684 0 0 0 4.91335 0 -88 0.509684 0 0 0 4.9949 0 -89 0.509684 0 0 0 5.05607 0 -90 0.509684 0 0 0 5.09684 0 -91 0.509684 0 0 0 5.158 0 -92 0.570846 0 0 0 5.21916 0 -93 0.632008 0 0 0 5.28033 0 -94 0.632008 0 0 0 5.34149 0 -95 0.632008 0 0 0 5.40265 0 -96 0.632008 0 0 0 5.46381 0 -97 0.632008 0 0 0 5.52497 0 -98 0.632008 0 0 0 5.52497 0 -99 0.632008 0 0 0 5.52497 0 -100 0.632008 0 0 0 5.52497 0 -101 0.632008 0 0 0 5.52497 0 -102 0.632008 0 0 0 5.52497 0 -103 0.632008 0 0 0 5.52497 0 -104 0.632008 0 0 0 5.52497 0 -105 0.632008 0 0 0 5.52497 0 -106 0.632008 0 0 0 5.52497 0 -107 0.632008 0 0 0 5.52497 0 -108 0.632008 0 0 0 5.52497 0 ->>END_MODULE diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_data_customlimits_summary.txt --- a/test-data/fastqc_data_customlimits_summary.txt Tue Sep 10 19:02:42 2024 +0000 +++ b/test-data/fastqc_data_customlimits_summary.txt Fri Sep 27 17:41:40 2024 +0000 @@ -1,11 +1,2 @@ PASS Basic Statistics 1000trimmed_fastq -PASS Per base sequence quality 1000trimmed_fastq -FAIL Per tile sequence quality 1000trimmed_fastq -PASS Per sequence quality scores 1000trimmed_fastq -FAIL Per base sequence content 1000trimmed_fastq -WARN Per sequence GC content 1000trimmed_fastq -PASS Per base N content 1000trimmed_fastq WARN Sequence Length Distribution 1000trimmed_fastq -PASS Sequence Duplication Levels 1000trimmed_fastq -WARN Overrepresented sequences 1000trimmed_fastq -PASS Adapter Content 1000trimmed_fastq diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_data_hisat.txt --- a/test-data/fastqc_data_hisat.txt Tue Sep 10 19:02:42 2024 +0000 +++ b/test-data/fastqc_data_hisat.txt Fri Sep 27 17:41:40 2024 +0000 @@ -1,448 +1,601 @@ -##FastQC 0.12.1 +##Falco 1.2.4 >>Basic Statistics pass #Measure Value Filename hisat_output_1_bam File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20 -Total Bases 1.4 kbp Sequences flagged as poor quality 0 Sequence length 70 -%GC 43 +%GC 44 >>END_MODULE ->>Per base sequence quality pass +>>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile -1 17.0 NaN NaN NaN NaN NaN -2 17.0 NaN NaN NaN NaN NaN -3 17.0 NaN NaN NaN NaN NaN -4 17.0 NaN NaN NaN NaN NaN -5 17.0 NaN NaN NaN NaN NaN -6 17.0 NaN NaN NaN NaN NaN -7 17.0 NaN NaN NaN NaN NaN -8 17.0 NaN NaN NaN NaN NaN -9 17.0 NaN NaN NaN NaN NaN -10 17.0 NaN NaN NaN NaN NaN -11 17.0 NaN NaN NaN NaN NaN -12 17.0 NaN NaN NaN NaN NaN -13 17.0 NaN NaN NaN NaN NaN -14 17.0 NaN NaN NaN NaN NaN -15 17.0 NaN NaN NaN NaN NaN -16 17.0 NaN NaN NaN NaN NaN -17 17.0 NaN NaN NaN NaN NaN -18 17.0 NaN NaN NaN NaN NaN -19 17.0 NaN NaN NaN NaN NaN -20 17.0 NaN NaN NaN NaN NaN -21 17.0 NaN NaN NaN NaN NaN -22 17.0 NaN NaN NaN NaN NaN -23 17.0 NaN NaN NaN NaN NaN -24 17.0 NaN NaN NaN NaN NaN -25 17.0 NaN NaN NaN NaN NaN -26 17.0 NaN NaN NaN NaN NaN -27 17.0 NaN NaN NaN NaN NaN -28 17.0 NaN NaN NaN NaN NaN -29 17.0 NaN NaN NaN NaN NaN -30 17.0 NaN NaN NaN NaN NaN -31 17.0 NaN NaN NaN NaN NaN -32 17.0 NaN NaN NaN NaN NaN -33 17.0 NaN NaN NaN NaN NaN -34 17.0 NaN NaN NaN NaN NaN -35 17.0 NaN NaN NaN NaN NaN -36 17.0 NaN NaN NaN NaN NaN -37 17.0 NaN NaN NaN NaN NaN -38 17.0 NaN NaN NaN NaN NaN -39 17.0 NaN NaN NaN NaN NaN -40 17.0 NaN NaN NaN NaN NaN -41 17.0 NaN NaN NaN NaN NaN -42 17.0 NaN NaN NaN NaN NaN -43 17.0 NaN NaN NaN NaN NaN -44 17.0 NaN NaN NaN NaN NaN -45 17.0 NaN NaN NaN NaN NaN -46 17.0 NaN NaN NaN NaN NaN -47 17.0 NaN NaN NaN NaN NaN -48 17.0 NaN NaN NaN NaN NaN -49 17.0 NaN NaN NaN NaN NaN -50 17.0 NaN NaN NaN NaN NaN -51 17.0 NaN NaN NaN NaN NaN -52 17.0 NaN NaN NaN NaN NaN -53 17.0 NaN NaN NaN NaN NaN -54 17.0 NaN NaN NaN NaN NaN -55 17.0 NaN NaN NaN NaN NaN -56 17.0 NaN NaN NaN NaN NaN -57 17.0 NaN NaN NaN NaN NaN -58 17.0 NaN NaN NaN NaN NaN -59 17.0 NaN NaN NaN NaN NaN -60 17.0 NaN NaN NaN NaN NaN -61 17.0 NaN NaN NaN NaN NaN -62 17.0 NaN NaN NaN NaN NaN -63 17.0 NaN NaN NaN NaN NaN -64 17.0 NaN NaN NaN NaN NaN -65 17.0 NaN NaN NaN NaN NaN -66 17.0 NaN NaN NaN NaN NaN -67 17.0 NaN NaN NaN NaN NaN -68 17.0 NaN NaN NaN NaN NaN -69 17.0 NaN NaN NaN NaN NaN -70 17.0 NaN NaN NaN NaN NaN +1 17 17 17 17 17 17 +2 17 17 17 17 17 17 +3 17 17 17 17 17 17 +4 17 17 17 17 17 17 +5 17 17 17 17 17 17 +6 17 17 17 17 17 17 +7 17 17 17 17 17 17 +8 17 17 17 17 17 17 +9 17 17 17 17 17 17 +10 17 17 17 17 17 17 +11 17 17 17 17 17 17 +12 17 17 17 17 17 17 +13 17 17 17 17 17 17 +14 17 17 17 17 17 17 +15 17 17 17 17 17 17 +16 17 17 17 17 17 17 +17 17 17 17 17 17 17 +18 17 17 17 17 17 17 +19 17 17 17 17 17 17 +20 17 17 17 17 17 17 +21 17 17 17 17 17 17 +22 17 17 17 17 17 17 +23 17 17 17 17 17 17 +24 17 17 17 17 17 17 +25 17 17 17 17 17 17 +26 17 17 17 17 17 17 +27 17 17 17 17 17 17 +28 17 17 17 17 17 17 +29 17 17 17 17 17 17 +30 17 17 17 17 17 17 +31 17 17 17 17 17 17 +32 17 17 17 17 17 17 +33 17 17 17 17 17 17 +34 17 17 17 17 17 17 +35 17 17 17 17 17 17 +36 17 17 17 17 17 17 +37 17 17 17 17 17 17 +38 17 17 17 17 17 17 +39 17 17 17 17 17 17 +40 17 17 17 17 17 17 +41 17 17 17 17 17 17 +42 17 17 17 17 17 17 +43 17 17 17 17 17 17 +44 17 17 17 17 17 17 +45 17 17 17 17 17 17 +46 17 17 17 17 17 17 +47 17 17 17 17 17 17 +48 17 17 17 17 17 17 +49 17 17 17 17 17 17 +50 17 17 17 17 17 17 +51 17 17 17 17 17 17 +52 17 17 17 17 17 17 +53 17 17 17 17 17 17 +54 17 17 17 17 17 17 +55 17 17 17 17 17 17 +56 17 17 17 17 17 17 +57 17 17 17 17 17 17 +58 17 17 17 17 17 17 +59 17 17 17 17 17 17 +60 17 17 17 17 17 17 +61 17 17 17 17 17 17 +62 17 17 17 17 17 17 +63 17 17 17 17 17 17 +64 17 17 17 17 17 17 +65 17 17 17 17 17 17 +66 17 17 17 17 17 17 +67 17 17 17 17 17 17 +68 17 17 17 17 17 17 +69 17 17 17 17 17 17 +70 17 17 17 17 17 17 +>>END_MODULE +>>Per tile sequence quality pass +#Tile Base Mean +470 1 0 +470 2 0 +470 3 0 +470 4 0 +470 5 0 +470 6 0 +470 7 0 +470 8 0 +470 9 0 +470 10 0 +470 11 0 +470 12 0 +470 13 0 +470 14 0 +470 15 0 +470 16 0 +470 17 0 +470 18 0 +470 19 0 +470 20 0 +470 21 0 +470 22 0 +470 23 0 +470 24 0 +470 25 0 +470 26 0 +470 27 0 +470 28 0 +470 29 0 +470 30 0 +470 31 0 +470 32 0 +470 33 0 +470 34 0 +470 35 0 +470 36 0 +470 37 0 +470 38 0 +470 39 0 +470 40 0 +470 41 0 +470 42 0 +470 43 0 +470 44 0 +470 45 0 +470 46 0 +470 47 0 +470 48 0 +470 49 0 +470 50 0 +470 51 0 +470 52 0 +470 53 0 +470 54 0 +470 55 0 +470 56 0 +470 57 0 +470 58 0 +470 59 0 +470 60 0 +470 61 0 +470 62 0 +470 63 0 +470 64 0 +470 65 0 +470 66 0 +470 67 0 +470 68 0 +470 69 0 +470 70 0 +473 1 0 +473 2 0 +473 3 0 +473 4 0 +473 5 0 +473 6 0 +473 7 0 +473 8 0 +473 9 0 +473 10 0 +473 11 0 +473 12 0 +473 13 0 +473 14 0 +473 15 0 +473 16 0 +473 17 0 +473 18 0 +473 19 0 +473 20 0 +473 21 0 +473 22 0 +473 23 0 +473 24 0 +473 25 0 +473 26 0 +473 27 0 +473 28 0 +473 29 0 +473 30 0 +473 31 0 +473 32 0 +473 33 0 +473 34 0 +473 35 0 +473 36 0 +473 37 0 +473 38 0 +473 39 0 +473 40 0 +473 41 0 +473 42 0 +473 43 0 +473 44 0 +473 45 0 +473 46 0 +473 47 0 +473 48 0 +473 49 0 +473 50 0 +473 51 0 +473 52 0 +473 53 0 +473 54 0 +473 55 0 +473 56 0 +473 57 0 +473 58 0 +473 59 0 +473 60 0 +473 61 0 +473 62 0 +473 63 0 +473 64 0 +473 65 0 +473 66 0 +473 67 0 +473 68 0 +473 69 0 +473 70 0 >>END_MODULE >>Per sequence quality scores fail #Quality Count -17 20.0 +17 20 >>END_MODULE >>Per base sequence content fail #Base G A T C -1 20.0 5.0 35.0 40.0 -2 10.0 10.0 45.0 35.0 -3 35.0 20.0 20.0 25.0 -4 35.0 30.0 25.0 10.0 -5 20.0 20.0 30.0 30.0 -6 20.0 35.0 20.0 25.0 -7 15.0 40.0 35.0 10.0 -8 20.0 15.0 45.0 20.0 -9 20.0 25.0 35.0 20.0 -10 20.0 20.0 30.0 30.0 -11 15.0 20.0 45.0 20.0 -12 10.0 40.0 35.0 15.0 -13 25.0 35.0 20.0 20.0 -14 35.0 20.0 20.0 25.0 -15 30.0 35.0 15.0 20.0 -16 10.0 45.0 25.0 20.0 -17 25.0 25.0 40.0 10.0 -18 25.0 35.0 10.0 30.0 -19 5.0 30.0 25.0 40.0 -20 20.0 15.0 40.0 25.0 -21 25.0 25.0 25.0 25.0 -22 15.0 30.0 20.0 35.0 -23 20.0 5.0 45.0 30.0 -24 10.0 30.0 35.0 25.0 -25 30.0 40.0 15.0 15.0 -26 15.0 35.0 20.0 30.0 -27 15.0 35.0 30.0 20.0 -28 25.0 25.0 30.0 20.0 -29 15.0 30.0 20.0 35.0 -30 20.0 35.0 30.0 15.0 -31 20.0 35.0 25.0 20.0 -32 35.0 15.0 35.0 15.0 -33 30.0 35.0 15.0 20.0 -34 25.0 25.0 25.0 25.0 -35 25.0 20.0 35.0 20.0 -36 30.0 25.0 20.0 25.0 -37 15.0 45.0 25.0 15.0 -38 30.0 25.0 35.0 10.0 -39 20.0 45.0 15.0 20.0 -40 15.0 35.0 20.0 30.0 -41 35.0 25.0 20.0 20.0 -42 30.0 30.0 35.0 5.0 -43 25.0 15.0 40.0 20.0 -44 40.0 20.0 30.0 10.0 -45 15.0 35.0 25.0 25.0 -46 15.0 30.0 40.0 15.0 -47 35.0 15.0 30.0 20.0 -48 30.0 35.0 20.0 15.0 -49 10.0 55.00000000000001 30.0 5.0 -50 40.0 25.0 20.0 15.0 -51 25.0 35.0 10.0 30.0 -52 30.0 25.0 20.0 25.0 -53 30.0 10.0 30.0 30.0 -54 20.0 40.0 20.0 20.0 -55 10.0 35.0 10.0 45.0 -56 50.0 10.0 30.0 10.0 -57 15.0 45.0 30.0 10.0 -58 20.0 35.0 20.0 25.0 -59 30.0 35.0 30.0 5.0 -60 20.0 35.0 25.0 20.0 -61 25.0 15.0 35.0 25.0 -62 10.0 20.0 55.00000000000001 15.0 -63 25.0 20.0 35.0 20.0 -64 20.0 35.0 25.0 20.0 -65 30.0 35.0 25.0 10.0 -66 15.0 40.0 35.0 10.0 -67 20.0 35.0 20.0 25.0 -68 20.0 25.0 30.0 25.0 -69 15.0 35.0 25.0 25.0 -70 5.0 40.0 40.0 15.0 +1 20 5 35 40 +2 10 10 45 35 +3 35 20 20 25 +4 35 30 25 10 +5 20 20 30 30 +6 20 35 20 25 +7 15 40 35 10 +8 20 15 45 20 +9 20 25 35 20 +10 20 20 30 30 +11 15 20 45 20 +12 10 40 35 15 +13 25 35 20 20 +14 35 20 20 25 +15 30 35 15 20 +16 10 45 25 20 +17 25 25 40 10 +18 25 35 10 30 +19 5 30 25 40 +20 20 15 40 25 +21 25 25 25 25 +22 15 30 20 35 +23 20 5 45 30 +24 10 30 35 25 +25 30 40 15 15 +26 15 35 20 30 +27 15 35 30 20 +28 25 25 30 20 +29 15 30 20 35 +30 20 35 30 15 +31 20 35 25 20 +32 35 15 35 15 +33 30 35 15 20 +34 25 25 25 25 +35 25 20 35 20 +36 30 25 20 25 +37 15 45 25 15 +38 30 25 35 10 +39 20 45 15 20 +40 15 35 20 30 +41 35 25 20 20 +42 30 30 35 5 +43 25 15 40 20 +44 40 20 30 10 +45 15 35 25 25 +46 15 30 40 15 +47 35 15 30 20 +48 30 35 20 15 +49 10 55 30 5 +50 40 25 20 15 +51 25 35 10 30 +52 30 25 20 25 +53 30 10 30 30 +54 20 40 20 20 +55 10 35 10 45 +56 50 10 30 10 +57 15 45 30 10 +58 20 35 20 25 +59 30 35 30 5 +60 20 35 25 20 +61 25 15 35 25 +62 10 20 55 15 +63 25 20 35 20 +64 20 35 25 20 +65 30 35 25 10 +66 15 40 35 10 +67 20 35 20 25 +68 20 25 30 25 +69 15 35 25 25 +70 5 40 40 15 >>END_MODULE >>Per sequence GC content fail #GC Content Count -0 0.0 -1 0.0 -2 0.0 -3 0.0 -4 0.0 -5 0.0 -6 0.0 -7 0.0 -8 0.0 -9 0.0 -10 0.0 -11 0.0 -12 0.0 -13 0.0 -14 0.0 -15 0.0 -16 0.0 -17 0.0 -18 0.0 -19 0.0 -20 0.0 -21 0.0 -22 0.0 -23 0.0 -24 0.0 -25 0.0 -26 0.0 -27 0.0 -28 0.0 +0 0 +1 0 +2 0 +3 0 +4 0 +5 0 +6 0 +7 0 +8 0 +9 0 +10 0 +11 0 +12 0 +13 0 +14 0 +15 0 +16 0 +17 0 +18 0 +19 0 +20 0 +21 0 +22 0 +23 0 +24 0 +25 0 +26 0 +27 0 +28 0 29 0.5 -30 1.0 +30 1 31 0.5 32 0.5 -33 1.0 +33 1 34 0.5 -35 0.0 -36 0.0 -37 0.0 -38 1.0 +35 0 +36 0 +37 0 +38 1 39 2.5 -40 3.0 -41 2.0 +40 3 +41 2 42 1.5 -43 2.0 +43 2 44 2.5 45 2.5 -46 1.0 -47 0.0 +46 1 +47 0 48 0.5 49 0.5 -50 0.0 +50 0 51 1.5 52 1.5 -53 0.0 +53 0 54 0.5 55 0.5 -56 0.0 -57 0.0 -58 0.0 -59 0.0 -60 0.0 -61 0.0 -62 0.0 -63 0.0 -64 0.0 -65 0.0 -66 0.0 -67 0.0 -68 0.0 -69 0.0 -70 0.0 -71 0.0 -72 0.0 -73 0.0 -74 0.0 -75 0.0 -76 0.0 -77 0.0 -78 0.0 -79 0.0 -80 0.0 -81 0.0 -82 0.0 -83 0.0 -84 0.0 -85 0.0 -86 0.0 -87 0.0 -88 0.0 -89 0.0 -90 0.0 -91 0.0 -92 0.0 -93 0.0 -94 0.0 -95 0.0 -96 0.0 -97 0.0 -98 0.0 -99 0.0 -100 0.0 +56 0 +57 0 +58 0 +59 0 +60 0 +61 0 +62 0 +63 0 +64 0 +65 0 +66 0 +67 0 +68 0 +69 0 +70 0 +71 0 +72 0 +73 0 +74 0 +75 0 +76 0 +77 0 +78 0 +79 0 +80 0 +81 0 +82 0 +83 0 +84 0 +85 0 +86 0 +87 0 +88 0 +89 0 +90 0 +91 0 +92 0 +93 0 +94 0 +95 0 +96 0 +97 0 +98 0 +99 0 +100 0 >>END_MODULE >>Per base N content pass #Base N-Count -1 0.0 -2 0.0 -3 0.0 -4 0.0 -5 0.0 -6 0.0 -7 0.0 -8 0.0 -9 0.0 -10 0.0 -11 0.0 -12 0.0 -13 0.0 -14 0.0 -15 0.0 -16 0.0 -17 0.0 -18 0.0 -19 0.0 -20 0.0 -21 0.0 -22 0.0 -23 0.0 -24 0.0 -25 0.0 -26 0.0 -27 0.0 -28 0.0 -29 0.0 -30 0.0 -31 0.0 -32 0.0 -33 0.0 -34 0.0 -35 0.0 -36 0.0 -37 0.0 -38 0.0 -39 0.0 -40 0.0 -41 0.0 -42 0.0 -43 0.0 -44 0.0 -45 0.0 -46 0.0 -47 0.0 -48 0.0 -49 0.0 -50 0.0 -51 0.0 -52 0.0 -53 0.0 -54 0.0 -55 0.0 -56 0.0 -57 0.0 -58 0.0 -59 0.0 -60 0.0 -61 0.0 -62 0.0 -63 0.0 -64 0.0 -65 0.0 -66 0.0 -67 0.0 -68 0.0 -69 0.0 -70 0.0 +1 0 +2 0 +3 0 +4 0 +5 0 +6 0 +7 0 +8 0 +9 0 +10 0 +11 0 +12 0 +13 0 +14 0 +15 0 +16 0 +17 0 +18 0 +19 0 +20 0 +21 0 +22 0 +23 0 +24 0 +25 0 +26 0 +27 0 +28 0 +29 0 +30 0 +31 0 +32 0 +33 0 +34 0 +35 0 +36 0 +37 0 +38 0 +39 0 +40 0 +41 0 +42 0 +43 0 +44 0 +45 0 +46 0 +47 0 +48 0 +49 0 +50 0 +51 0 +52 0 +53 0 +54 0 +55 0 +56 0 +57 0 +58 0 +59 0 +60 0 +61 0 +62 0 +63 0 +64 0 +65 0 +66 0 +67 0 +68 0 +69 0 +70 0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 70 20.0 >>END_MODULE >>Sequence Duplication Levels pass -#Total Deduplicated Percentage 100.0 -#Duplication Level Percentage of total -1 100.0 -2 0.0 -3 0.0 -4 0.0 -5 0.0 -6 0.0 -7 0.0 -8 0.0 -9 0.0 ->10 0.0 ->50 0.0 ->100 0.0 ->500 0.0 ->1k 0.0 ->5k 0.0 ->10k+ 0.0 +#Total Deduplicated Percentage 100 +#Duplication Level Percentage of deduplicated Percentage of total +1 100 100 +2 0 0 +3 0 0 +4 0 0 +5 0 0 +6 0 0 +7 0 0 +8 0 0 +9 0 0 +>10 0 0 +>50 0 0 +>100 0 0 +>500 0 0 +>1k 0 0 +>5k 0 0 +>10k+ 0 0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source -CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAG 1 5.0 No Hit -TGGCGCTCTCCGTCTTTCTCCATTTCGTCGTGGCCTTGCTATTGACTCTA 1 5.0 No Hit -ACCATAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTAG 1 5.0 No Hit -TGTTTTCCGTAAATTCAGCGCCTTCCATGATGCGACAGGCCGTTTGAATG 1 5.0 No Hit -CTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT 1 5.0 No Hit -TCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGAGTC 1 5.0 No Hit -CCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCACCTTT 1 5.0 No Hit -TAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTCAAG 1 5.0 No Hit -CAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAG 1 5.0 No Hit -GCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAG 1 5.0 No Hit -CTGAATGGAATTAAGAAAACCACCAATACCAGCATTAACCTTCAAACTAT 1 5.0 No Hit -GCGACCATTCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAA 1 5.0 No Hit -GTGAAATTTCTAGGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAG 1 5.0 No Hit -CTCAAATCCGGCGTCAACCATACCAGCATAGGAAGCATCAGCACCAGCAC 1 5.0 No Hit -TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACC 1 5.0 No Hit -CTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATA 1 5.0 No Hit -TTAGGTGTGTGTAAAACAGGTGCCGAAGAAGCTGGATTAACAGAATTGAG 1 5.0 No Hit -GCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGA 1 5.0 No Hit -TTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGTAATT 1 5.0 No Hit -CTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAA 1 5.0 No Hit +ACCATAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTC 1 5 No Hit +GTGAAATTTCTAGGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGTCA 1 5 No Hit +CCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCT 1 5 No Hit +CTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGCA 1 5 No Hit +CAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCATTTGGCTAGAA 1 5 No Hit +CTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAAAT 1 5 No Hit +TGGCGCTCTCCGTCTTTCTCCATTTCGTCGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTT 1 5 No Hit +TAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTCAAGCGCCGAGGATGCGTGACCGT 1 5 No Hit +TGTTTTCCGTAAATTCAGCGCCTTCCATGATGCGACAGGCCGTTTGAATGTTGACGGGATGAACATAATA 1 5 No Hit +TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACCAGTGTTTCCAGTCCGTTCAG 1 5 No Hit +TCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGAGTCATTTCATTGATTTGGTCATT 1 5 No Hit +GCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGC 1 5 No Hit +CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCT 1 5 No Hit +TTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGTAATTACTACTGCTTGTTTACGAAT 1 5 No Hit +TTAGGTGTGTGTAAAACAGGTGCCGAAGAAGCTGGATTAACAGAATTGAGAACCAGCTTATCAGAAAAAA 1 5 No Hit +CTGAATGGAATTAAGAAAACCACCAATACCAGCATTAACCTTCAAACTATCAAAATATAACGTTGACGAT 1 5 No Hit +GCGACCATTCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAACCGACGAAGACTCAAAGCGA 1 5 No Hit +GCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAAAGCCGCCTC 1 5 No Hit +CTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAA 1 5 No Hit +CTCAAATCCGGCGTCAACCATACCAGCATAGGAAGCATCAGCACCAGCACGCTCCCAAGCATTAATCTCA 1 5 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG -1 0.0 0.0 0.0 0.0 0.0 0.0 -2 0.0 0.0 0.0 0.0 0.0 0.0 -3 0.0 0.0 0.0 0.0 0.0 0.0 -4 0.0 0.0 0.0 0.0 0.0 0.0 -5 0.0 0.0 0.0 0.0 0.0 0.0 -6 0.0 0.0 0.0 0.0 0.0 0.0 -7 0.0 0.0 0.0 0.0 0.0 0.0 -8 0.0 0.0 0.0 0.0 0.0 0.0 -9 0.0 0.0 0.0 0.0 0.0 0.0 -10 0.0 0.0 0.0 0.0 0.0 0.0 -11 0.0 0.0 0.0 0.0 0.0 0.0 -12 0.0 0.0 0.0 0.0 0.0 0.0 -13 0.0 0.0 0.0 0.0 0.0 0.0 -14 0.0 0.0 0.0 0.0 0.0 0.0 -15 0.0 0.0 0.0 0.0 0.0 0.0 -16 0.0 0.0 0.0 0.0 0.0 0.0 -17 0.0 0.0 0.0 0.0 0.0 0.0 -18 0.0 0.0 0.0 0.0 0.0 0.0 -19 0.0 0.0 0.0 0.0 0.0 0.0 -20 0.0 0.0 0.0 0.0 0.0 0.0 -21 0.0 0.0 0.0 0.0 0.0 0.0 -22 0.0 0.0 0.0 0.0 0.0 0.0 -23 0.0 0.0 0.0 0.0 0.0 0.0 -24 0.0 0.0 0.0 0.0 0.0 0.0 -25 0.0 0.0 0.0 0.0 0.0 0.0 -26 0.0 0.0 0.0 0.0 0.0 0.0 -27 0.0 0.0 0.0 0.0 0.0 0.0 -28 0.0 0.0 0.0 0.0 0.0 0.0 -29 0.0 0.0 0.0 0.0 0.0 0.0 -30 0.0 0.0 0.0 0.0 0.0 0.0 -31 0.0 0.0 0.0 0.0 0.0 0.0 -32 0.0 0.0 0.0 0.0 0.0 0.0 -33 0.0 0.0 0.0 0.0 0.0 0.0 -34 0.0 0.0 0.0 0.0 0.0 0.0 -35 0.0 0.0 0.0 0.0 0.0 0.0 -36 0.0 0.0 0.0 0.0 0.0 0.0 -37 0.0 0.0 0.0 0.0 0.0 0.0 -38 0.0 0.0 0.0 0.0 0.0 0.0 -39 0.0 0.0 0.0 0.0 0.0 0.0 -40 0.0 0.0 0.0 0.0 0.0 0.0 -41 0.0 0.0 0.0 0.0 0.0 0.0 -42 0.0 0.0 0.0 0.0 0.0 0.0 -43 0.0 0.0 0.0 0.0 0.0 0.0 -44 0.0 0.0 0.0 0.0 0.0 0.0 -45 0.0 0.0 0.0 0.0 0.0 0.0 -46 0.0 0.0 0.0 0.0 0.0 0.0 -47 0.0 0.0 0.0 0.0 0.0 0.0 -48 0.0 0.0 0.0 0.0 0.0 0.0 -49 0.0 0.0 0.0 0.0 0.0 0.0 -50 0.0 0.0 0.0 0.0 0.0 0.0 -51 0.0 0.0 0.0 0.0 0.0 0.0 -52 0.0 0.0 0.0 0.0 0.0 0.0 -53 0.0 0.0 0.0 0.0 0.0 0.0 -54 0.0 0.0 0.0 0.0 0.0 0.0 -55 0.0 0.0 0.0 0.0 0.0 0.0 -56 0.0 0.0 0.0 0.0 0.0 0.0 -57 0.0 0.0 0.0 0.0 0.0 0.0 -58 0.0 0.0 0.0 0.0 0.0 0.0 -59 0.0 0.0 0.0 0.0 0.0 0.0 +1 0 0 0 0 0 0 +2 0 0 0 0 0 0 +3 0 0 0 0 0 0 +4 0 0 0 0 0 0 +5 0 0 0 0 0 0 +6 0 0 0 0 0 0 +7 0 0 0 0 0 0 +8 0 0 0 0 0 0 +9 0 0 0 0 0 0 +10 0 0 0 0 0 0 +11 0 0 0 0 0 0 +12 0 0 0 0 0 0 +13 0 0 0 0 0 0 +14 0 0 0 0 0 0 +15 0 0 0 0 0 0 +16 0 0 0 0 0 0 +17 0 0 0 0 0 0 +18 0 0 0 0 0 0 +19 0 0 0 0 0 0 +20 0 0 0 0 0 0 +21 0 0 0 0 0 0 +22 0 0 0 0 0 0 +23 0 0 0 0 0 0 +24 0 0 0 0 0 0 +25 0 0 0 0 0 0 +26 0 0 0 0 0 0 +27 0 0 0 0 0 0 +28 0 0 0 0 0 0 +29 0 0 0 0 0 0 +30 0 0 0 0 0 0 +31 0 0 0 0 0 0 +32 0 0 0 0 0 0 +33 0 0 0 0 0 0 +34 0 0 0 0 0 0 +35 0 0 0 0 0 0 +36 0 0 0 0 0 0 +37 0 0 0 0 0 0 +38 0 0 0 0 0 0 +39 0 0 0 0 0 0 +40 0 0 0 0 0 0 +41 0 0 0 0 0 0 +42 0 0 0 0 0 0 +43 0 0 0 0 0 0 +44 0 0 0 0 0 0 +45 0 0 0 0 0 0 +46 0 0 0 0 0 0 +47 0 0 0 0 0 0 +48 0 0 0 0 0 0 +49 0 0 0 0 0 0 +50 0 0 0 0 0 0 +51 0 0 0 0 0 0 +52 0 0 0 0 0 0 +53 0 0 0 0 0 0 +54 0 0 0 0 0 0 +55 0 0 0 0 0 0 +56 0 0 0 0 0 0 +57 0 0 0 0 0 0 +58 0 0 0 0 0 0 +59 0 0 0 0 0 0 +60 0 0 0 0 0 0 +61 0 0 0 0 0 0 +62 0 0 0 0 0 0 +63 0 0 0 0 0 0 +64 0 0 0 0 0 0 +65 0 0 0 0 0 0 +66 0 0 0 0 0 0 +67 0 0 0 0 0 0 +68 0 0 0 0 0 0 +69 0 0 0 0 0 0 +70 0 0 0 0 0 0 >>END_MODULE diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_data_nogroup.txt --- a/test-data/fastqc_data_nogroup.txt Tue Sep 10 19:02:42 2024 +0000 +++ b/test-data/fastqc_data_nogroup.txt Fri Sep 27 17:41:40 2024 +0000 @@ -1,4 +1,4 @@ -##Falco 1.2.3 +##Falco 1.2.4 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq @@ -1744,114 +1744,114 @@ #Sequence Count Percentage Possible Source ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672783 No Hit >>END_MODULE ->>Adapter Content warn +>>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG 1 0 0 0 0 0.0203874 0 -2 0 0 0 0 0.0815494 0 -3 0 0 0 0 0.142712 0 -4 0 0 0 0 0.183486 0 -5 0 0 0 0 0.285423 0 -6 0 0 0 0 0.38736 0 -7 0 0 0 0 0.489297 0 -8 0 0 0 0 0.591233 0 -9 0 0 0 0 0.672783 0 -10 0 0 0 0 0.754332 0 -11 0 0 0 0 0.835882 0 -12 0 0 0 0 0.917431 0 -13 0 0 0 0 1.01937 0 -14 0 0 0 0 1.1213 0 -15 0 0 0 0 1.24363 0 -16 0 0 0 0 1.34557 0 -17 0 0 0 0 1.46789 0 -18 0 0 0 0 1.59021 0 -19 0 0 0 0 1.67176 0 -20 0.122324 0 0 0 1.75331 0 -21 0.122324 0 0 0 1.83486 0 -22 0.122324 0 0 0 1.89602 0 -23 0.122324 0 0 0 1.95719 0 -24 0.122324 0 0 0 2.01835 0 -25 0.122324 0 0 0 2.07951 0 -26 0.122324 0 0 0 2.14067 0 -27 0.142712 0 0 0 2.20183 0 -28 0.183486 0 0 0 2.263 0 -29 0.224261 0 0 0 2.32416 0 -30 0.224261 0 0 0 2.38532 0 -31 0.224261 0 0 0 2.44648 0 -32 0.224261 0 0 0 2.50765 0 -33 0.224261 0 0 0 2.60958 0 -34 0.224261 0 0 0 2.71152 0 -35 0.265036 0 0 0 2.81346 0 -36 0.285423 0 0 0 2.89501 0 -37 0.326198 0 0 0 2.99694 0 -38 0.407747 0 0 0 3.09888 0 -39 0.468909 0 0 0 3.20082 0 -40 0.468909 0 0 0 3.30275 0 -41 0.468909 0 0 0 3.40469 0 -42 0.468909 0 0 0 3.48624 0 -43 0.468909 0 0 0 3.56779 0 -44 0.468909 0 0 0 3.60856 0 -45 0.468909 0 0 0 3.62895 0 -46 0.468909 0 0 0 3.64934 0 -47 0.468909 0 0 0 3.66972 0 -48 0.468909 0 0 0 3.69011 0 -49 0.468909 0 0 0 3.7105 0 -50 0.468909 0 0 0 3.7105 0 -51 0.468909 0 0 0 3.7105 0 -52 0.468909 0 0 0 3.7105 0 -53 0.468909 0 0 0 3.7105 0 -54 0.468909 0 0 0 3.7105 0 -55 0.468909 0 0 0 3.7105 0 -56 0.468909 0 0 0 3.7105 0 -57 0.468909 0 0 0 3.7105 0 -58 0.468909 0 0 0 3.7105 0 -59 0.468909 0 0 0 3.73089 0 -60 0.468909 0 0 0 3.75127 0 -61 0.468909 0 0 0 3.77166 0 -62 0.468909 0 0 0 3.81244 0 -63 0.468909 0 0 0 3.85321 0 -64 0.468909 0 0 0 3.89399 0 -65 0.468909 0 0 0 3.93476 0 -66 0.468909 0 0 0 3.97554 0 -67 0.468909 0 0 0 4.01631 0 -68 0.468909 0 0 0 4.05708 0 -69 0.468909 0 0 0 4.09786 0 -70 0.468909 0 0 0 4.13863 0 -71 0.468909 0 0 0 4.17941 0 -72 0.468909 0 0 0 4.22018 0 -73 0.468909 0 0 0 4.26096 0 -74 0.489297 0 0 0 4.32212 0 -75 0.489297 0 0 0 4.38328 0 -76 0.489297 0 0 0 4.42406 0 -77 0.489297 0 0 0 4.46483 0 -78 0.489297 0 0 0 4.50561 0 -79 0.489297 0 0 0 4.54638 0 -80 0.489297 0 0 0 4.58716 0 -81 0.489297 0 0 0 4.62793 0 -82 0.489297 0 0 0 4.66871 0 -83 0.509684 0 0 0 4.70948 0 -84 0.509684 0 0 0 4.75025 0 -85 0.509684 0 0 0 4.79103 0 -86 0.509684 0 0 0 4.8318 0 -87 0.509684 0 0 0 4.91335 0 -88 0.509684 0 0 0 4.9949 0 -89 0.509684 0 0 0 5.05607 0 -90 0.509684 0 0 0 5.09684 0 -91 0.509684 0 0 0 5.158 0 -92 0.570846 0 0 0 5.21916 0 -93 0.632008 0 0 0 5.28033 0 -94 0.632008 0 0 0 5.34149 0 -95 0.632008 0 0 0 5.40265 0 -96 0.632008 0 0 0 5.46381 0 -97 0.632008 0 0 0 5.52497 0 -98 0.632008 0 0 0 5.52497 0 -99 0.632008 0 0 0 5.52497 0 -100 0.632008 0 0 0 5.52497 0 -101 0.632008 0 0 0 5.52497 0 -102 0.632008 0 0 0 5.52497 0 -103 0.632008 0 0 0 5.52497 0 -104 0.632008 0 0 0 5.52497 0 -105 0.632008 0 0 0 5.52497 0 -106 0.632008 0 0 0 5.52497 0 -107 0.632008 0 0 0 5.52497 0 -108 0.632008 0 0 0 5.52497 0 +2 0 0 0 0 0.0611621 0 +3 0 0 0 0 0.0611621 0 +4 0 0 0 0 0.0611621 0 +5 0 0 0 0 0.122324 0 +6 0 0 0 0 0.122324 0 +7 0 0 0 0 0.122324 0 +8 0 0 0 0 0.142712 0 +9 0 0 0 0 0.142712 0 +10 0 0 0 0 0.142712 0 +11 0 0 0 0 0.142712 0 +12 0 0 0 0 0.142712 0 +13 0 0 0 0 0.163099 0 +14 0 0 0 0 0.163099 0 +15 0 0 0 0 0.183486 0 +16 0 0 0 0 0.203874 0 +17 0 0 0 0 0.224261 0 +18 0 0 0 0 0.224261 0 +19 0 0 0 0 0.224261 0 +20 0.122324 0 0 0 0.244648 0 +21 0.122324 0 0 0 0.244648 0 +22 0.122324 0 0 0 0.244648 0 +23 0.122324 0 0 0 0.244648 0 +24 0.122324 0 0 0 0.244648 0 +25 0.122324 0 0 0 0.244648 0 +26 0.122324 0 0 0 0.244648 0 +27 0.142712 0 0 0 0.244648 0 +28 0.183486 0 0 0 0.244648 0 +29 0.224261 0 0 0 0.244648 0 +30 0.224261 0 0 0 0.244648 0 +31 0.224261 0 0 0 0.244648 0 +32 0.224261 0 0 0 0.244648 0 +33 0.224261 0 0 0 0.285423 0 +34 0.224261 0 0 0 0.285423 0 +35 0.265036 0 0 0 0.30581 0 +36 0.285423 0 0 0 0.30581 0 +37 0.326198 0 0 0 0.326198 0 +38 0.407747 0 0 0 0.326198 0 +39 0.468909 0 0 0 0.326198 0 +40 0.468909 0 0 0 0.326198 0 +41 0.468909 0 0 0 0.326198 0 +42 0.468909 0 0 0 0.326198 0 +43 0.468909 0 0 0 0.326198 0 +44 0.468909 0 0 0 0.326198 0 +45 0.468909 0 0 0 0.326198 0 +46 0.468909 0 0 0 0.326198 0 +47 0.468909 0 0 0 0.326198 0 +48 0.468909 0 0 0 0.326198 0 +49 0.468909 0 0 0 0.326198 0 +50 0.468909 0 0 0 0.326198 0 +51 0.468909 0 0 0 0.326198 0 +52 0.468909 0 0 0 0.326198 0 +53 0.468909 0 0 0 0.326198 0 +54 0.468909 0 0 0 0.326198 0 +55 0.468909 0 0 0 0.326198 0 +56 0.468909 0 0 0 0.326198 0 +57 0.468909 0 0 0 0.326198 0 +58 0.468909 0 0 0 0.326198 0 +59 0.468909 0 0 0 0.326198 0 +60 0.468909 0 0 0 0.326198 0 +61 0.468909 0 0 0 0.326198 0 +62 0.468909 0 0 0 0.326198 0 +63 0.468909 0 0 0 0.326198 0 +64 0.468909 0 0 0 0.326198 0 +65 0.468909 0 0 0 0.326198 0 +66 0.468909 0 0 0 0.326198 0 +67 0.468909 0 0 0 0.326198 0 +68 0.468909 0 0 0 0.326198 0 +69 0.468909 0 0 0 0.326198 0 +70 0.468909 0 0 0 0.326198 0 +71 0.468909 0 0 0 0.326198 0 +72 0.468909 0 0 0 0.326198 0 +73 0.468909 0 0 0 0.326198 0 +74 0.468909 0 0 0 0.326198 0 +75 0.468909 0 0 0 0.326198 0 +76 0.468909 0 0 0 0.326198 0 +77 0.468909 0 0 0 0.326198 0 +78 0.468909 0 0 0 0.326198 0 +79 0.468909 0 0 0 0.326198 0 +80 0.468909 0 0 0 0.326198 0 +81 0.468909 0 0 0 0.326198 0 +82 0.468909 0 0 0 0.326198 0 +83 0.468909 0 0 0 0.326198 0 +84 0.468909 0 0 0 0.326198 0 +85 0.468909 0 0 0 0.326198 0 +86 0.468909 0 0 0 0.326198 0 +87 0.468909 0 0 0 0.326198 0 +88 0.468909 0 0 0 0.326198 0 +89 0.468909 0 0 0 0.326198 0 +90 0.468909 0 0 0 0.326198 0 +91 0.468909 0 0 0 0.326198 0 +92 0.468909 0 0 0 0.326198 0 +93 0.468909 0 0 0 0.326198 0 +94 0.468909 0 0 0 0.326198 0 +95 0.468909 0 0 0 0.326198 0 +96 0.468909 0 0 0 0.326198 0 +97 0.468909 0 0 0 0.326198 0 +98 0.468909 0 0 0 0.326198 0 +99 0.468909 0 0 0 0.326198 0 +100 0.468909 0 0 0 0.326198 0 +101 0.468909 0 0 0 0.326198 0 +102 0.468909 0 0 0 0.326198 0 +103 0.468909 0 0 0 0.326198 0 +104 0.468909 0 0 0 0.326198 0 +105 0.468909 0 0 0 0.326198 0 +106 0.468909 0 0 0 0.326198 0 +107 0.468909 0 0 0 0.326198 0 +108 0.468909 0 0 0 0.326198 0 >>END_MODULE diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_data_nogroup_summary.txt --- a/test-data/fastqc_data_nogroup_summary.txt Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,11 +0,0 @@ -PASS Basic Statistics 1000trimmed_fastq -PASS Per base sequence quality 1000trimmed_fastq -FAIL Per tile sequence quality 1000trimmed_fastq -PASS Per sequence quality scores 1000trimmed_fastq -FAIL Per base sequence content 1000trimmed_fastq -WARN Per sequence GC content 1000trimmed_fastq -PASS Per base N content 1000trimmed_fastq -WARN Sequence Length Distribution 1000trimmed_fastq -PASS Sequence Duplication Levels 1000trimmed_fastq -WARN Overrepresented sequences 1000trimmed_fastq -WARN Adapter Content 1000trimmed_fastq diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_data_summary.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastqc_data_summary.txt Fri Sep 27 17:41:40 2024 +0000 @@ -0,0 +1,11 @@ +PASS Basic Statistics 1000trimmed_fastq +PASS Per base sequence quality 1000trimmed_fastq +FAIL Per tile sequence quality 1000trimmed_fastq +PASS Per sequence quality scores 1000trimmed_fastq +FAIL Per base sequence content 1000trimmed_fastq +WARN Per sequence GC content 1000trimmed_fastq +PASS Per base N content 1000trimmed_fastq +WARN Sequence Length Distribution 1000trimmed_fastq +PASS Sequence Duplication Levels 1000trimmed_fastq +WARN Overrepresented sequences 1000trimmed_fastq +PASS Adapter Content 1000trimmed_fastq diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report.html --- a/test-data/fastqc_report.html Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ - 1000trimmed_fastq - report
Report
Sun Sep 1 15:39:03 2024 -
1000trimmed_fastq

Summary

Basic Statistics: pass

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences Flagged As Poor Quality0
Sequence length1 - 108
%GC:41

Per base sequence quality: pass

Per tile sequence quality : fail

Per sequence quality scores : pass

Per base sequence content : fail

Per sequence GC content: warn

Per base N content : pass

Sequence Length Distribution : warn

Sequence Duplication Levels : pass

Overrepresented sequences : warn

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672783No Hit

Adapter Content : warn

Falco 1.2.3
\ No newline at end of file diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_adapters.html --- a/test-data/fastqc_report_adapters.html Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ - 1000trimmed_fastq - report
Report
Sun Sep 1 15:39:42 2024 -
1000trimmed_fastq

Summary

Basic Statistics: pass

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences Flagged As Poor Quality0
Sequence length1 - 108
%GC:41

Per base sequence quality: pass

Per tile sequence quality : fail

Per sequence quality scores : pass

Per base sequence content : fail

Per sequence GC content: warn

Per base N content : pass

Sequence Length Distribution : warn

Sequence Duplication Levels : pass

Overrepresented sequences : warn

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672783No Hit

Adapter Content : pass

Falco 1.2.3
\ No newline at end of file diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_bisulfite.html --- a/test-data/fastqc_report_bisulfite.html Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ - 1000trimmed_fastq - report
Report
Sun Sep 1 15:40:53 2024 -
1000trimmed_fastq

Summary

Basic Statistics: pass

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences Flagged As Poor Quality0
Sequence length1 - 108
%GC:41

Per base sequence quality: pass

Per tile sequence quality : fail

Per sequence quality scores : pass

Per base sequence content : warn

Per sequence GC content: warn

Per base N content : pass

Sequence Length Distribution : warn

Sequence Duplication Levels : pass

Overrepresented sequences : warn

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672783No Hit

Adapter Content : warn

Falco 1.2.3
\ No newline at end of file diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_bisulfite.txt --- a/test-data/fastqc_report_bisulfite.txt Tue Sep 10 19:02:42 2024 +0000 +++ b/test-data/fastqc_report_bisulfite.txt Fri Sep 27 17:41:40 2024 +0000 @@ -1,4 +1,4 @@ -##Falco 1.2.3 +##Falco 1.2.4 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq @@ -1597,114 +1597,114 @@ #Sequence Count Percentage Possible Source ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672783 No Hit >>END_MODULE ->>Adapter Content warn +>>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG 1 0 0 0 0 0.0203874 0 -2 0 0 0 0 0.0815494 0 -3 0 0 0 0 0.142712 0 -4 0 0 0 0 0.183486 0 -5 0 0 0 0 0.285423 0 -6 0 0 0 0 0.38736 0 -7 0 0 0 0 0.489297 0 -8 0 0 0 0 0.591233 0 -9 0 0 0 0 0.672783 0 -10 0 0 0 0 0.754332 0 -11 0 0 0 0 0.835882 0 -12 0 0 0 0 0.917431 0 -13 0 0 0 0 1.01937 0 -14 0 0 0 0 1.1213 0 -15 0 0 0 0 1.24363 0 -16 0 0 0 0 1.34557 0 -17 0 0 0 0 1.46789 0 -18 0 0 0 0 1.59021 0 -19 0 0 0 0 1.67176 0 -20 0.122324 0 0 0 1.75331 0 -21 0.122324 0 0 0 1.83486 0 -22 0.122324 0 0 0 1.89602 0 -23 0.122324 0 0 0 1.95719 0 -24 0.122324 0 0 0 2.01835 0 -25 0.122324 0 0 0 2.07951 0 -26 0.122324 0 0 0 2.14067 0 -27 0.142712 0 0 0 2.20183 0 -28 0.183486 0 0 0 2.263 0 -29 0.224261 0 0 0 2.32416 0 -30 0.224261 0 0 0 2.38532 0 -31 0.224261 0 0 0 2.44648 0 -32 0.224261 0 0 0 2.50765 0 -33 0.224261 0 0 0 2.60958 0 -34 0.224261 0 0 0 2.71152 0 -35 0.265036 0 0 0 2.81346 0 -36 0.285423 0 0 0 2.89501 0 -37 0.326198 0 0 0 2.99694 0 -38 0.407747 0 0 0 3.09888 0 -39 0.468909 0 0 0 3.20082 0 -40 0.468909 0 0 0 3.30275 0 -41 0.468909 0 0 0 3.40469 0 -42 0.468909 0 0 0 3.48624 0 -43 0.468909 0 0 0 3.56779 0 -44 0.468909 0 0 0 3.60856 0 -45 0.468909 0 0 0 3.62895 0 -46 0.468909 0 0 0 3.64934 0 -47 0.468909 0 0 0 3.66972 0 -48 0.468909 0 0 0 3.69011 0 -49 0.468909 0 0 0 3.7105 0 -50 0.468909 0 0 0 3.7105 0 -51 0.468909 0 0 0 3.7105 0 -52 0.468909 0 0 0 3.7105 0 -53 0.468909 0 0 0 3.7105 0 -54 0.468909 0 0 0 3.7105 0 -55 0.468909 0 0 0 3.7105 0 -56 0.468909 0 0 0 3.7105 0 -57 0.468909 0 0 0 3.7105 0 -58 0.468909 0 0 0 3.7105 0 -59 0.468909 0 0 0 3.73089 0 -60 0.468909 0 0 0 3.75127 0 -61 0.468909 0 0 0 3.77166 0 -62 0.468909 0 0 0 3.81244 0 -63 0.468909 0 0 0 3.85321 0 -64 0.468909 0 0 0 3.89399 0 -65 0.468909 0 0 0 3.93476 0 -66 0.468909 0 0 0 3.97554 0 -67 0.468909 0 0 0 4.01631 0 -68 0.468909 0 0 0 4.05708 0 -69 0.468909 0 0 0 4.09786 0 -70 0.468909 0 0 0 4.13863 0 -71 0.468909 0 0 0 4.17941 0 -72 0.468909 0 0 0 4.22018 0 -73 0.468909 0 0 0 4.26096 0 -74 0.489297 0 0 0 4.32212 0 -75 0.489297 0 0 0 4.38328 0 -76 0.489297 0 0 0 4.42406 0 -77 0.489297 0 0 0 4.46483 0 -78 0.489297 0 0 0 4.50561 0 -79 0.489297 0 0 0 4.54638 0 -80 0.489297 0 0 0 4.58716 0 -81 0.489297 0 0 0 4.62793 0 -82 0.489297 0 0 0 4.66871 0 -83 0.509684 0 0 0 4.70948 0 -84 0.509684 0 0 0 4.75025 0 -85 0.509684 0 0 0 4.79103 0 -86 0.509684 0 0 0 4.8318 0 -87 0.509684 0 0 0 4.91335 0 -88 0.509684 0 0 0 4.9949 0 -89 0.509684 0 0 0 5.05607 0 -90 0.509684 0 0 0 5.09684 0 -91 0.509684 0 0 0 5.158 0 -92 0.570846 0 0 0 5.21916 0 -93 0.632008 0 0 0 5.28033 0 -94 0.632008 0 0 0 5.34149 0 -95 0.632008 0 0 0 5.40265 0 -96 0.632008 0 0 0 5.46381 0 -97 0.632008 0 0 0 5.52497 0 -98 0.632008 0 0 0 5.52497 0 -99 0.632008 0 0 0 5.52497 0 -100 0.632008 0 0 0 5.52497 0 -101 0.632008 0 0 0 5.52497 0 -102 0.632008 0 0 0 5.52497 0 -103 0.632008 0 0 0 5.52497 0 -104 0.632008 0 0 0 5.52497 0 -105 0.632008 0 0 0 5.52497 0 -106 0.632008 0 0 0 5.52497 0 -107 0.632008 0 0 0 5.52497 0 -108 0.632008 0 0 0 5.52497 0 +2 0 0 0 0 0.0611621 0 +3 0 0 0 0 0.0611621 0 +4 0 0 0 0 0.0611621 0 +5 0 0 0 0 0.122324 0 +6 0 0 0 0 0.122324 0 +7 0 0 0 0 0.122324 0 +8 0 0 0 0 0.142712 0 +9 0 0 0 0 0.142712 0 +10 0 0 0 0 0.142712 0 +11 0 0 0 0 0.142712 0 +12 0 0 0 0 0.142712 0 +13 0 0 0 0 0.163099 0 +14 0 0 0 0 0.163099 0 +15 0 0 0 0 0.183486 0 +16 0 0 0 0 0.203874 0 +17 0 0 0 0 0.224261 0 +18 0 0 0 0 0.224261 0 +19 0 0 0 0 0.224261 0 +20 0.122324 0 0 0 0.244648 0 +21 0.122324 0 0 0 0.244648 0 +22 0.122324 0 0 0 0.244648 0 +23 0.122324 0 0 0 0.244648 0 +24 0.122324 0 0 0 0.244648 0 +25 0.122324 0 0 0 0.244648 0 +26 0.122324 0 0 0 0.244648 0 +27 0.142712 0 0 0 0.244648 0 +28 0.183486 0 0 0 0.244648 0 +29 0.224261 0 0 0 0.244648 0 +30 0.224261 0 0 0 0.244648 0 +31 0.224261 0 0 0 0.244648 0 +32 0.224261 0 0 0 0.244648 0 +33 0.224261 0 0 0 0.285423 0 +34 0.224261 0 0 0 0.285423 0 +35 0.265036 0 0 0 0.30581 0 +36 0.285423 0 0 0 0.30581 0 +37 0.326198 0 0 0 0.326198 0 +38 0.407747 0 0 0 0.326198 0 +39 0.468909 0 0 0 0.326198 0 +40 0.468909 0 0 0 0.326198 0 +41 0.468909 0 0 0 0.326198 0 +42 0.468909 0 0 0 0.326198 0 +43 0.468909 0 0 0 0.326198 0 +44 0.468909 0 0 0 0.326198 0 +45 0.468909 0 0 0 0.326198 0 +46 0.468909 0 0 0 0.326198 0 +47 0.468909 0 0 0 0.326198 0 +48 0.468909 0 0 0 0.326198 0 +49 0.468909 0 0 0 0.326198 0 +50 0.468909 0 0 0 0.326198 0 +51 0.468909 0 0 0 0.326198 0 +52 0.468909 0 0 0 0.326198 0 +53 0.468909 0 0 0 0.326198 0 +54 0.468909 0 0 0 0.326198 0 +55 0.468909 0 0 0 0.326198 0 +56 0.468909 0 0 0 0.326198 0 +57 0.468909 0 0 0 0.326198 0 +58 0.468909 0 0 0 0.326198 0 +59 0.468909 0 0 0 0.326198 0 +60 0.468909 0 0 0 0.326198 0 +61 0.468909 0 0 0 0.326198 0 +62 0.468909 0 0 0 0.326198 0 +63 0.468909 0 0 0 0.326198 0 +64 0.468909 0 0 0 0.326198 0 +65 0.468909 0 0 0 0.326198 0 +66 0.468909 0 0 0 0.326198 0 +67 0.468909 0 0 0 0.326198 0 +68 0.468909 0 0 0 0.326198 0 +69 0.468909 0 0 0 0.326198 0 +70 0.468909 0 0 0 0.326198 0 +71 0.468909 0 0 0 0.326198 0 +72 0.468909 0 0 0 0.326198 0 +73 0.468909 0 0 0 0.326198 0 +74 0.468909 0 0 0 0.326198 0 +75 0.468909 0 0 0 0.326198 0 +76 0.468909 0 0 0 0.326198 0 +77 0.468909 0 0 0 0.326198 0 +78 0.468909 0 0 0 0.326198 0 +79 0.468909 0 0 0 0.326198 0 +80 0.468909 0 0 0 0.326198 0 +81 0.468909 0 0 0 0.326198 0 +82 0.468909 0 0 0 0.326198 0 +83 0.468909 0 0 0 0.326198 0 +84 0.468909 0 0 0 0.326198 0 +85 0.468909 0 0 0 0.326198 0 +86 0.468909 0 0 0 0.326198 0 +87 0.468909 0 0 0 0.326198 0 +88 0.468909 0 0 0 0.326198 0 +89 0.468909 0 0 0 0.326198 0 +90 0.468909 0 0 0 0.326198 0 +91 0.468909 0 0 0 0.326198 0 +92 0.468909 0 0 0 0.326198 0 +93 0.468909 0 0 0 0.326198 0 +94 0.468909 0 0 0 0.326198 0 +95 0.468909 0 0 0 0.326198 0 +96 0.468909 0 0 0 0.326198 0 +97 0.468909 0 0 0 0.326198 0 +98 0.468909 0 0 0 0.326198 0 +99 0.468909 0 0 0 0.326198 0 +100 0.468909 0 0 0 0.326198 0 +101 0.468909 0 0 0 0.326198 0 +102 0.468909 0 0 0 0.326198 0 +103 0.468909 0 0 0 0.326198 0 +104 0.468909 0 0 0 0.326198 0 +105 0.468909 0 0 0 0.326198 0 +106 0.468909 0 0 0 0.326198 0 +107 0.468909 0 0 0 0.326198 0 +108 0.468909 0 0 0 0.326198 0 >>END_MODULE diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_bisulfite_summary.txt --- a/test-data/fastqc_report_bisulfite_summary.txt Tue Sep 10 19:02:42 2024 +0000 +++ b/test-data/fastqc_report_bisulfite_summary.txt Fri Sep 27 17:41:40 2024 +0000 @@ -8,4 +8,4 @@ WARN Sequence Length Distribution 1000trimmed_fastq PASS Sequence Duplication Levels 1000trimmed_fastq WARN Overrepresented sequences 1000trimmed_fastq -WARN Adapter Content 1000trimmed_fastq +PASS Adapter Content 1000trimmed_fastq diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_contaminants.html --- a/test-data/fastqc_report_contaminants.html Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ - 1000trimmed_fastq - report
Report
Sun Sep 1 15:39:23 2024 -
1000trimmed_fastq

Summary

Basic Statistics: pass

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences Flagged As Poor Quality0
Sequence length1 - 108
%GC:41

Per base sequence quality: pass

Per tile sequence quality : fail

Per sequence quality scores : pass

Per base sequence content : fail

Per sequence GC content: warn

Per base N content : pass

Sequence Length Distribution : warn

Sequence Duplication Levels : pass

Overrepresented sequences : warn

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672783No Hit

Adapter Content : warn

Falco 1.2.3
\ No newline at end of file diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_customlimits.html --- a/test-data/fastqc_report_customlimits.html Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ - 1000trimmed_fastq - report
Report
Sun Sep 1 15:40:01 2024 -
1000trimmed_fastq

Summary

Basic Statistics: pass

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences Flagged As Poor Quality0
Sequence length1 - 108
%GC:41

Per base sequence quality: pass

Per tile sequence quality : fail

Per sequence quality scores : pass

Per base sequence content : fail

Per sequence GC content: warn

Per base N content : pass

Sequence Length Distribution : warn

Sequence Duplication Levels : pass

Overrepresented sequences : warn

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672783No Hit

Adapter Content : warn

Falco 1.2.3
\ No newline at end of file diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_hisat.html --- a/test-data/fastqc_report_hisat.html Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,187 +0,0 @@ -hisat_output_1_bam FastQC Report
FastQCFastQC Report
Thu 8 Jun 2023
hisat_output_1_bam

Summary

[OK]Basic Statistics

MeasureValue
Filenamehisat_output_1_bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Total Bases1.4 kbp
Sequences flagged as poor quality0
Sequence length70
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAG15.0No Hit
TGGCGCTCTCCGTCTTTCTCCATTTCGTCGTGGCCTTGCTATTGACTCTA15.0No Hit
ACCATAAACGCAAGCCTCAACGCAGCGACGAGCACGAGAGCGGTCAGTAG15.0No Hit
TGTTTTCCGTAAATTCAGCGCCTTCCATGATGCGACAGGCCGTTTGAATG15.0No Hit
CTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT15.0No Hit
TCTGCGTTTGCTGATGAACTAAGTCAACCTCAGCACTAACCTTGCGAGTC15.0No Hit
CCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCACCTTT15.0No Hit
TAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTCAAG15.0No Hit
CAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAG15.0No Hit
GCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAG15.0No Hit
CTGAATGGAATTAAGAAAACCACCAATACCAGCATTAACCTTCAAACTAT15.0No Hit
GCGACCATTCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAA15.0No Hit
GTGAAATTTCTAGGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAG15.0No Hit
CTCAAATCCGGCGTCAACCATACCAGCATAGGAAGCATCAGCACCAGCAC15.0No Hit
TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGACC15.0No Hit
CTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATA15.0No Hit
TTAGGTGTGTGTAAAACAGGTGCCGAAGAAGCTGGATTAACAGAATTGAG15.0No Hit
GCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGA15.0No Hit
TTTCGGATATTTCTGATGAGTCGAAAAATTATCTTGATAAAGCAGTAATT15.0No Hit
CTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAA15.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)
\ No newline at end of file diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_kmer.html --- a/test-data/fastqc_report_kmer.html Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ - 1000trimmed_fastq - report
Report
Mon May 27 16:59:30 2024 -
1000trimmed_fastq

Summary

Basic Statistics: pass

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences Flagged As Poor Quality0
Sequence length1 - 108
%GC:41

Per base sequence quality: pass

Per tile sequence quality : fail

Per sequence quality scores : pass

Per base sequence content : fail

Per sequence GC content: warn

Per base N content : pass

Sequence Length Distribution : warn

Sequence Duplication Levels : pass

Overrepresented sequences : warn

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672783No Hit

Adapter Content : pass

Falco 1.2.2
\ No newline at end of file diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_min_length.html --- a/test-data/fastqc_report_min_length.html Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ - 1000trimmed_fastq - report
Report
Mon May 27 17:01:42 2024 -
1000trimmed_fastq

Summary

Basic Statistics: pass

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences Flagged As Poor Quality0
Sequence length1 - 108
%GC:41

Per base sequence quality: pass

Per tile sequence quality : fail

Per sequence quality scores : pass

Per base sequence content : fail

Per sequence GC content: warn

Per base N content : pass

Sequence Length Distribution : warn

Sequence Duplication Levels : pass

Overrepresented sequences : warn

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672783No Hit

Adapter Content : pass

Falco 1.2.2
\ No newline at end of file diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_nogroup.html --- a/test-data/fastqc_report_nogroup.html Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ - 1000trimmed_fastq - report
Report
Sun Sep 1 15:40:19 2024 -
1000trimmed_fastq

Summary

Basic Statistics: pass

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences Flagged As Poor Quality0
Sequence length1 - 108
%GC:41

Per base sequence quality: pass

Per tile sequence quality : fail

Per sequence quality scores : pass

Per base sequence content : fail

Per sequence GC content: warn

Per base N content : pass

Sequence Length Distribution : warn

Sequence Duplication Levels : pass

Overrepresented sequences : warn

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672783No Hit

Adapter Content : warn

Falco 1.2.3
\ No newline at end of file diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_reverse_complement.html --- a/test-data/fastqc_report_reverse_complement.html Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ - 1000trimmed_fastq - report
Report
Sun Sep 1 15:41:08 2024 -
1000trimmed_fastq

Summary

Basic Statistics: pass

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences Flagged As Poor Quality0
Sequence length1 - 108
%GC:41

Per base sequence quality: pass

Per tile sequence quality : fail

Per sequence quality scores : pass

Per base sequence content : fail

Per sequence GC content: warn

Per base N content : pass

Sequence Length Distribution : warn

Sequence Duplication Levels : pass

Overrepresented sequences : warn

SequenceCountPercentagePossible Source
ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT330.672783No Hit

Adapter Content : warn

Falco 1.2.3
\ No newline at end of file diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_reverse_complement.txt --- a/test-data/fastqc_report_reverse_complement.txt Tue Sep 10 19:02:42 2024 +0000 +++ b/test-data/fastqc_report_reverse_complement.txt Fri Sep 27 17:41:40 2024 +0000 @@ -1,4 +1,4 @@ -##Falco 1.2.3 +##Falco 1.2.4 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq @@ -1597,114 +1597,114 @@ #Sequence Count Percentage Possible Source ATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCATGCAT 33 0.672783 No Hit >>END_MODULE ->>Adapter Content warn +>>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG 1 0 0 0 0 0.0203874 0 -2 0 0 0 0 0.0815494 0 -3 0 0 0 0 0.142712 0 -4 0 0 0 0 0.183486 0 -5 0 0 0 0 0.285423 0 -6 0 0 0 0 0.38736 0 -7 0 0 0 0 0.489297 0 -8 0 0 0 0 0.591233 0 -9 0 0 0 0 0.672783 0 -10 0 0 0 0 0.754332 0 -11 0 0 0 0 0.835882 0 -12 0 0 0 0 0.917431 0 -13 0 0 0 0 1.01937 0 -14 0 0 0 0 1.1213 0 -15 0 0 0 0 1.24363 0 -16 0 0 0 0 1.34557 0 -17 0 0 0 0 1.46789 0 -18 0 0 0 0 1.59021 0 -19 0 0 0 0 1.67176 0 -20 0.122324 0 0 0 1.75331 0 -21 0.122324 0 0 0 1.83486 0 -22 0.122324 0 0 0 1.89602 0 -23 0.122324 0 0 0 1.95719 0 -24 0.122324 0 0 0 2.01835 0 -25 0.122324 0 0 0 2.07951 0 -26 0.122324 0 0 0 2.14067 0 -27 0.142712 0 0 0 2.20183 0 -28 0.183486 0 0 0 2.263 0 -29 0.224261 0 0 0 2.32416 0 -30 0.224261 0 0 0 2.38532 0 -31 0.224261 0 0 0 2.44648 0 -32 0.224261 0 0 0 2.50765 0 -33 0.224261 0 0 0 2.60958 0 -34 0.224261 0 0 0 2.71152 0 -35 0.265036 0 0 0 2.81346 0 -36 0.285423 0 0 0 2.89501 0 -37 0.326198 0 0 0 2.99694 0 -38 0.407747 0 0 0 3.09888 0 -39 0.468909 0 0 0 3.20082 0 -40 0.468909 0 0 0 3.30275 0 -41 0.468909 0 0 0 3.40469 0 -42 0.468909 0 0 0 3.48624 0 -43 0.468909 0 0 0 3.56779 0 -44 0.468909 0 0 0 3.60856 0 -45 0.468909 0 0 0 3.62895 0 -46 0.468909 0 0 0 3.64934 0 -47 0.468909 0 0 0 3.66972 0 -48 0.468909 0 0 0 3.69011 0 -49 0.468909 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0.509684 0 0 0 4.75025 0 -85 0.509684 0 0 0 4.79103 0 -86 0.509684 0 0 0 4.8318 0 -87 0.509684 0 0 0 4.91335 0 -88 0.509684 0 0 0 4.9949 0 -89 0.509684 0 0 0 5.05607 0 -90 0.509684 0 0 0 5.09684 0 -91 0.509684 0 0 0 5.158 0 -92 0.570846 0 0 0 5.21916 0 -93 0.632008 0 0 0 5.28033 0 -94 0.632008 0 0 0 5.34149 0 -95 0.632008 0 0 0 5.40265 0 -96 0.632008 0 0 0 5.46381 0 -97 0.632008 0 0 0 5.52497 0 -98 0.632008 0 0 0 5.52497 0 -99 0.632008 0 0 0 5.52497 0 -100 0.632008 0 0 0 5.52497 0 -101 0.632008 0 0 0 5.52497 0 -102 0.632008 0 0 0 5.52497 0 -103 0.632008 0 0 0 5.52497 0 -104 0.632008 0 0 0 5.52497 0 -105 0.632008 0 0 0 5.52497 0 -106 0.632008 0 0 0 5.52497 0 -107 0.632008 0 0 0 5.52497 0 -108 0.632008 0 0 0 5.52497 0 +2 0 0 0 0 0.0611621 0 +3 0 0 0 0 0.0611621 0 +4 0 0 0 0 0.0611621 0 +5 0 0 0 0 0.122324 0 +6 0 0 0 0 0.122324 0 +7 0 0 0 0 0.122324 0 +8 0 0 0 0 0.142712 0 +9 0 0 0 0 0.142712 0 +10 0 0 0 0 0.142712 0 +11 0 0 0 0 0.142712 0 +12 0 0 0 0 0.142712 0 +13 0 0 0 0 0.163099 0 +14 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2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,11 +0,0 @@ -PASS Basic Statistics 1000trimmed_fastq -PASS Per base sequence quality 1000trimmed_fastq -FAIL Per tile sequence quality 1000trimmed_fastq -PASS Per sequence quality scores 1000trimmed_fastq -FAIL Per base sequence content 1000trimmed_fastq -WARN Per sequence GC content 1000trimmed_fastq -PASS Per base N content 1000trimmed_fastq -WARN Sequence Length Distribution 1000trimmed_fastq -PASS Sequence Duplication Levels 1000trimmed_fastq -WARN Overrepresented sequences 1000trimmed_fastq -WARN Adapter Content 1000trimmed_fastq diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_subsample.html --- a/test-data/fastqc_report_subsample.html Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2 +0,0 @@ - 1000trimmed_fastq - report
Report
Sun Sep 1 15:40:37 2024 -
1000trimmed_fastq

Summary

Basic Statistics: pass

MeasureValue
Filename1000trimmed_fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4905
Sequences Flagged As Poor Quality0
Sequence length1 - 108
%GC:41

Per base sequence quality: pass

Per tile sequence quality : fail

Per sequence quality scores : pass

Per base sequence content : fail

Per sequence GC content: warn

Per base N content : pass

Sequence Length Distribution : warn

Sequence Duplication Levels : pass

Overrepresented sequences : pass

No overrepresented sequences

Adapter Content : pass

Falco 1.2.3
\ No newline at end of file diff -r babbcf02d35c -r 959a14c1f2dd test-data/fastqc_report_subsample.txt --- a/test-data/fastqc_report_subsample.txt Tue Sep 10 19:02:42 2024 +0000 +++ b/test-data/fastqc_report_subsample.txt Fri Sep 27 17:41:40 2024 +0000 @@ -1,4 +1,4 @@ -##Falco 1.2.3 +##Falco 1.2.4 >>Basic Statistics pass #Measure Value Filename 1000trimmed_fastq @@ -1593,110 +1593,110 @@ #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG 1 0 0 0 0 0 0 2 0 0 0 0 0.0203874 0 -3 0 0 0 0 0.0407747 0 -4 0 0 0 0 0.0407747 0 -5 0 0 0 0 0.0407747 0 -6 0 0 0 0 0.0407747 0 -7 0 0 0 0 0.0407747 0 -8 0 0 0 0 0.0407747 0 -9 0 0 0 0 0.0407747 0 -10 0 0 0 0 0.0407747 0 -11 0 0 0 0 0.0407747 0 -12 0 0 0 0 0.0407747 0 -13 0 0 0 0 0.0407747 0 -14 0 0 0 0 0.0407747 0 -15 0 0 0 0 0.0407747 0 -16 0 0 0 0 0.0407747 0 -17 0 0 0 0 0.0611621 0 -18 0 0 0 0 0.0815494 0 -19 0 0 0 0 0.0815494 0 -20 0.0203874 0 0 0 0.0815494 0 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0.0611621 0 0 0 0.0407747 0 +107 0.0611621 0 0 0 0.0407747 0 +108 0.0611621 0 0 0 0.0407747 0 >>END_MODULE diff -r babbcf02d35c -r 959a14c1f2dd test-data/limits.txt --- a/test-data/limits.txt Tue Sep 10 19:02:42 2024 +0000 +++ b/test-data/limits.txt Fri Sep 27 17:41:40 2024 +0000 @@ -1,81 +1,81 @@ -# For each of the modules you can choose to not run that -# module at all by setting the value below to 1 for the -# modules you want to remove. -duplication ignore 0 -kmer ignore 1 -n_content ignore 0 -overrepresented ignore 0 -quality_base ignore 0 -sequence ignore 0 -gc_sequence ignore 0 -quality_sequence ignore 0 -tile ignore 0 -sequence_length ignore 0 -adapter ignore 0 - -# For the duplication module the value is the percentage -# remaining after deduplication. Measured levels below -# these limits trigger the warning / error. -duplication warn 70 -duplication error 50 - -# For the kmer module the filter is on the -log10 binomial -# pvalue for the most significant Kmer, so 5 would be -# 10^-5 = p<0.00001 -kmer warn 2 -kmer error 5 - -# For the N module the filter is on the percentage of Ns -# at any position in the library -n_content warn 5 -n_content error 20 - -# For the overrepresented seqs the warn value sets the -# threshold for the overrepresented sequences to be reported -# at all as the proportion of the library which must be seen -# as a single sequence -overrepresented warn 0.1 -overrepresented error 1 - -# The per base quality filter uses two values, one for the value -# of the lower quartile, and the other for the value of the -# median quality. Failing either of these will trigger the alert -quality_base_lower warn 10 -quality_base_lower error 5 -quality_base_median warn 25 -quality_base_median error 20 - -# The per base sequence content module tests the maximum deviation -# between A and T or C and G -sequence warn 10 -sequence error 20 - -# The per sequence GC content tests the maximum deviation between -# the theoretical distribution and the real distribution -gc_sequence warn 15 -gc_sequence error 30 - -# The per sequence quality module tests the phred score which is -# most frequently observed -quality_sequence warn 27 -quality_sequence error 20 - -# The per tile module tests the maximum phred score loss between -# and individual tile and the average for that base across all tiles -tile warn 5 -tile error 10 - -# The sequence length module tests are binary, so the values here -# simply turn them on or off. The actual tests warn if you have -# sequences of different length, and error if you have sequences -# of zero length. - -sequence_length warn 1 -sequence_length error 1 - -# The adapter module's warnings and errors are based on the -# percentage of reads in the library which have been observed -# to contain an adapter associated Kmer at any point - -adapter warn 5 -adapter error 10 +# For each of the modules you can choose to not run that +# module at all by setting the value below to 1 for the +# modules you want to remove. +duplication ignore 1 +kmer ignore 1 +n_content ignore 1 +overrepresented ignore 1 +quality_base ignore 1 +sequence ignore 1 +gc_sequence ignore 1 +quality_sequence ignore 1 +tile ignore 1 +sequence_length ignore 0 +adapter ignore 1 + +# For the duplication module the value is the percentage +# remaining after deduplication. Measured levels below +# these limits trigger the warning / error. +duplication warn 70 +duplication error 50 + +# For the kmer module the filter is on the -log10 binomial +# pvalue for the most significant Kmer, so 5 would be +# 10^-5 = p<0.00001 +kmer warn 2 +kmer error 5 + +# For the N module the filter is on the percentage of Ns +# at any position in the library +n_content warn 5 +n_content error 20 + +# For the overrepresented seqs the warn value sets the +# threshold for the overrepresented sequences to be reported +# at all as the proportion of the library which must be seen +# as a single sequence +overrepresented warn 0.1 +overrepresented error 1 + +# The per base quality filter uses two values, one for the value +# of the lower quartile, and the other for the value of the +# median quality. Failing either of these will trigger the alert +quality_base_lower warn 10 +quality_base_lower error 5 +quality_base_median warn 25 +quality_base_median error 20 + +# The per base sequence content module tests the maximum deviation +# between A and T or C and G +sequence warn 10 +sequence error 20 + +# The per sequence GC content tests the maximum deviation between +# the theoretical distribution and the real distribution +gc_sequence warn 15 +gc_sequence error 30 + +# The per sequence quality module tests the phred score which is +# most frequently observed +quality_sequence warn 27 +quality_sequence error 20 + +# The per tile module tests the maximum phred score loss between +# and individual tile and the average for that base across all tiles +tile warn 5 +tile error 10 + +# The sequence length module tests are binary, so the values here +# simply turn them on or off. The actual tests warn if you have +# sequences of different length, and error if you have sequences +# of zero length. + +sequence_length warn 1 +sequence_length error 1 + +# The adapter module's warnings and errors are based on the +# percentage of reads in the library which have been observed +# to contain an adapter associated Kmer at any point + +adapter warn 5 +adapter error 10 diff -r babbcf02d35c -r 959a14c1f2dd test-data/summary.txt --- a/test-data/summary.txt Tue Sep 10 19:02:42 2024 +0000 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,11 +0,0 @@ -PASS Basic Statistics 1000trimmed_fastq -PASS Per base sequence quality 1000trimmed_fastq -FAIL Per tile sequence quality 1000trimmed_fastq -PASS Per sequence quality scores 1000trimmed_fastq -FAIL Per base sequence content 1000trimmed_fastq -WARN Per sequence GC content 1000trimmed_fastq -PASS Per base N content 1000trimmed_fastq -WARN Sequence Length Distribution 1000trimmed_fastq -PASS Sequence Duplication Levels 1000trimmed_fastq -WARN Overrepresented sequences 1000trimmed_fastq -PASS Adapter Content 1000trimmed_fastq