comparison test-data/paired/retrievedFragments/trimmedReads/reads__2_retrieved.fastq_trimming_report.txt @ 0:6f743c615c41 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/fargene commit 867e4a6fad4c2622ad69517e2d4d9ba185109b72"
author iuc
date Thu, 28 Nov 2019 14:39:41 -0500
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-1:000000000000 0:6f743c615c41
1
2 SUMMARISING RUN PARAMETERS
3 ==========================
4 Input filename: /home/ubuntu/data/fargene/tutorial/tutorialdata/tutorial_output_1/retrievedFragments/reads__2_retrieved.fastq
5 Trimming mode: paired-end
6 Trim Galore version: 0.6.3
7 Cutadapt version: 1.18
8 Number of cores used for trimming: 1
9 Quality Phred score cutoff: 30
10 Quality encoding type selected: ASCII+33
11 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection))
12 Maximum trimming error rate: 0.1 (default)
13 Minimum required adapter overlap (stringency): 1 bp
14 Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp
15
16
17 This is cutadapt 1.18 with Python 2.7.15
18 Command line parameters: -j 1 -e 0.1 -q 30 -O 1 -a AGATCGGAAGAGC /home/ubuntu/data/fargene/tutorial/tutorialdata/tutorial_output_1/retrievedFragments/reads__2_retrieved.fastq
19 Processing reads on 1 core in single-end mode ...
20 Finished in 0.01 s (28 us/read; 2.17 M reads/minute).
21
22 === Summary ===
23
24 Total reads processed: 393
25 Reads with adapters: 91 (23.2%)
26 Reads written (passing filters): 393 (100.0%)
27
28 Total basepairs processed: 39,300 bp
29 Quality-trimmed: 435 bp (1.1%)
30 Total written (filtered): 38,735 bp (98.6%)
31
32 === Adapter 1 ===
33
34 Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 91 times.
35
36 No. of allowed errors:
37 0-9 bp: 0; 10-13 bp: 1
38
39 Bases preceding removed adapters:
40 A: 20.9%
41 C: 44.0%
42 G: 27.5%
43 T: 7.7%
44 none/other: 0.0%
45
46 Overview of removed sequences
47 length count expect max.err error counts
48 1 65 98.2 0 65
49 2 20 24.6 0 20
50 3 2 6.1 0 2
51 4 2 1.5 0 2
52 5 1 0.4 0 1
53 6 1 0.1 0 1
54
55
56 RUN STATISTICS FOR INPUT FILE: /home/ubuntu/data/fargene/tutorial/tutorialdata/tutorial_output_1/retrievedFragments/reads__2_retrieved.fastq
57 =============================================
58 393 sequences processed in total
59
60 Total number of sequences analysed for the sequence pair length validation: 393
61
62 Number of sequence pairs removed because at least one read was shorter than the length cutoff (20 bp): 0 (0.00%)