annotate gemini_comp_hets.xml @ 4:62647e2637f6 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gemini commit 283362494058ed64143b1f27afb447b8a1cb4313
author iuc
date Fri, 14 Dec 2018 12:49:22 -0500
parents ed0b0677dcfe
children
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62647e2637f6 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/gemini commit 283362494058ed64143b1f27afb447b8a1cb4313
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1 <tool id="gemini_@BINARY@" name="GEMINI @BINARY@" version="@VERSION@.1">
0
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2 <description>Identifying potential compound heterozygotes</description>
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3 <macros>
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4 <import>gemini_macros.xml</import>
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5 <token name="@BINARY@">comp_hets</token>
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6 </macros>
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7 <expand macro="requirements" />
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8 <expand macro="stdio" />
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9 <expand macro="version_command" />
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10 <command>
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11 <![CDATA[
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12 gemini @BINARY@
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13
2
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14 @COLUMN_SELECT@
0
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15
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16 @CMDLN_SQL_FILTER_FILTER_OPTION@
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17
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18 #if int($min_kindreds) > 0:
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19 --min-kindreds $min_kindreds
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20 #end if
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21
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22 #if str($families).strip():
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23 --families "$families"
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24 #end if
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25
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26 -d $d
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27 $allow_unaffected
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28
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29 #if int($min_genotypequality) > 0:
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30 --min-gq $min_genotypequality
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31 #end if
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32
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33 #if int($gt_pl_max) != -1:
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34 --gt_pl_max $gt_pl_max
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35 #end if
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36
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37 $pattern_only
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38 --max-priority $max_priority
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39
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40
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41 "${ infile }"
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42 > "${ outfile }"
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43 ]]>
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44 </command>
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45 <inputs>
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46 <expand macro="infile" />
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47 <expand macro="column_filter" />
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48 <expand macro="filter" />
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49 <expand macro="min_kindreds" />
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50 <expand macro="family" />
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51 <expand macro="unaffected" />
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52 <expand macro="min_sequence_depth" />
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53 <param name="min_genotypequality" type="integer" value="0" min="0" label="The minimum genotype quality required for each sample in a family." help="default: 0 (--min-gq)" />
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54 <param name="gt_pl_max" type="integer" value="-1" min="-1" label="The maximum phred-scaled genotype (PL) allowed for each sample in a family." help="default: -1 not set (--gt-pl-max)" />
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55 <param name="pattern_only" type="boolean" truevalue="--pattern-only" falsevalue="" checked="False"
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56 label="Find compound hets by inheritance pattern, without regard to affection" help="(--pattern-only)"/>
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57 <param name="max_priority" type="integer" value="1" min="1" label="Default is to show only confident compound hets. Set to 2 or higher to include pairs that are less likely true comp-hets." help="default: 1 (--max-priority)" />
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58 </inputs>
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59 <outputs>
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60 <data name="outfile" format="tabular" />
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61 </outputs>
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62 <tests>
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63 <test>
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64 <param name="infile" value="gemini_comphets_input.db" ftype="gemini.sqlite" />
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65 <conditional name="report">
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66 <param name="report_selector" value="column_filter" />
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67 <param name="columns" value="chrom,start,end,ref,alt,gene,impact" />
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68 </conditional>
0
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69 <param name="allow_unaffected" value="True" />
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70 <param name="max_priority" value="3" />
4
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71 <output name="outfile">
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72 <assert_contents>
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73 <has_line_matching expression="chrom&#009;start&#009;end&#009;.*gene.*" />
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74 </assert_contents>
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75 </output>
0
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76 </test>
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77 </tests>
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78 <help>
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79 **What it does**
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80
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81 Many recessive disorders are caused by compound heterozygotes. Unlike canonical recessive sites where the same recessive allele is
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82 inherited from both parents at the _same_ site in the gene, compound heterozygotes occur when the individual’s phenotype is caused
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83 by two heterozygous recessive alleles at _different_ sites in a particular gene.
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84
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85 So basically, we are looking for two (typically loss-of-function (LoF)) heterozygous variants impacting the same gene at different loci.
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86 The complicating factor is that this is _recessive_ and as such, we must also require that the consequential alleles at each heterozygous
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87 site were inherited on different chromosomes (one from each parent). As such, in order to use this tool, we require that all variants are phased.
d666cc4a37e7 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/gemini commit 4bbfca6f0e9cae9a8f263aad4eab7304c96358c4
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88 Once this has been done, the comp_hets tool will provide a report of candidate compound heterozygotes for each sample/gene.
d666cc4a37e7 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/gemini commit 4bbfca6f0e9cae9a8f263aad4eab7304c96358c4
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89
d666cc4a37e7 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/gemini commit 4bbfca6f0e9cae9a8f263aad4eab7304c96358c4
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90
d666cc4a37e7 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/gemini commit 4bbfca6f0e9cae9a8f263aad4eab7304c96358c4
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91 </help>
d666cc4a37e7 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/gemini commit 4bbfca6f0e9cae9a8f263aad4eab7304c96358c4
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92 <expand macro="citations"/>
d666cc4a37e7 planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/gemini commit 4bbfca6f0e9cae9a8f263aad4eab7304c96358c4
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93 </tool>