diff generate_test_data @ 0:ba4dfbdc1295 draft

planemo upload for repository https://github.com/ASaiM/galaxytools/tree/master/tools/humann2/ commit b46aa969c01b7e5f4f133192899fa4da286ecf89-dirty
author iuc
date Mon, 13 Mar 2017 12:38:56 -0400
parents
children dbf0e8eeeeed
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/generate_test_data	Mon Mar 13 12:38:56 2017 -0400
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+#/usr/bin/env bash
+
+# humann2
+humann2 \
+    --input 'test-data/input_sequences.fasta' \
+    -o 'humann2_output' \
+    --annotation-gene-index 8 \
+    --taxonomic-profile 'test-data/taxonomic_profile.tabular' \
+    --metaphlan-options="-t rel_ab" \
+    --nucleotide-database 'test-data/' \
+    --protein-database 'test-data/' \
+    --evalue '1' \
+    --search-mode 'uniref50' \
+    --prescreen-threshold '0.01' \
+    --identity-threshold '50' \
+    --translated-subject-coverage-threshold '50' \
+    --translated-query-coverage-threshold '50' \
+    --translated-alignment 'diamond' \
+    --xipe 'off' \
+    --minpath 'on' \
+    --pick-frames 'on' \
+    --gap-fill 'off' \
+    --output-format 'tsv' \
+    --output-max-decimals '10' \
+    --output-basename 'humann2' \
+    --pathways 'metacyc'
+
+humann2 \
+    --input 'test-data/input_sequences.fasta' \
+    -o 'humann2_output' \
+    --annotation-gene-index 8 \
+    --metaphlan-options="-t rel_ab" \
+    --nucleotide-database 'test-data/' \
+    --protein-database 'test-data/' \
+    --evalue '1' \
+    --search-mode 'uniref90' \
+    --prescreen-threshold '0.01' \
+    --identity-threshold '50' \
+    --translated-subject-coverage-threshold '50' \
+    --translated-query-coverage-threshold '50' \
+    --translated-alignment 'rapsearch' \
+    --xipe 'off' \
+    --minpath 'on' \
+    --pick-frames 'on' \
+    --gap-fill 'off' \
+    --output-format 'tsv' \
+    --output-max-decimals '10' \
+    --output-basename 'humann2' \
+    --pathways 'unipathway'
+
+# humann2_regroup_table
+humann2_regroup_table \
+    --input 'test-data/demo_genefamilies.tsv' \
+    -o 'test-data/regrouped_gene_families_to_infogo1000.tsv' \
+    --function 'sum' \
+    --groups 'uniref90_infogo1000' \
+    --ungrouped 'Y' \
+    --protected 'Y'
+
+humann2_regroup_table \
+    --input 'test-data/demo_genefamilies.tsv' \
+    -o 'test-data/regrouped_gene_families_to_ko.tsv' \
+    --function 'mean' \
+    --groups 'uniref90_ko' \
+    --ungrouped 'Y' \
+    --protected 'Y'
+
+humann2_regroup_table \
+    --input 'test-data/demo_genefamilies.tsv' \
+    -o 'test-data/regrouped_gene_families_to_rxn.tsv' \
+    --function 'sum' \
+    --groups 'uniref90_rxn' \
+    --ungrouped 'Y' \
+    --protected 'Y'
+
+# humann2_renorm_table
+humann2_renorm_table \
+    --input 'test-data/demo_pathabundance.tsv' \
+    -o 'test-data/cpm_community_renormalized_pathway_abundance.tsv' \
+    --units 'cpm' \
+    --mode 'community' \
+    --special 'n'
+
+humann2_renorm_table \
+    --input 'test-data/demo_pathabundance.tsv' \
+    -o 'test-data/relab_levelwise_renormalized_pathway_abundance.tsv' \
+    --units 'relab' \
+    --mode 'levelwise' \
+    --special 'y'
+
+# humann2_join_tables
+mkdir join_table_tmp_dir
+cp 'test-data/demo_pathabundance.tsv' join_table_tmp_dir
+cp 'test-data/demo_pathcoverage.tsv' join_table_tmp_dir
+humann2_join_tables \
+    -i 'join_table_tmp_dir' \
+    -o 'test-data/joined_pathway_coverage_abundance.tsv'
+
+# humann2_merge_abundance_tables
+humann2_merge_abundance_tables \
+    --input-genes 'test-data/demo_genefamilies.tsv' \
+    --input-pathways 'test-data/demo_pathabundance.tsv' \
+    -o 'test-data/merged_gene_families_pathways_abundances.tsv' 
+
+# humann2_reduce_table
+humann2_reduce_table \
+    --input 'test-data/demo_genefamilies.tsv' \
+    -o 'test-data/max_reduced_gene_family_abundance.tsv' \
+    --function 'max' \
+    --sort-by 'name'
+
+# humann2_rename_table
+humann2_rename_table \
+    --input 'test-data/demo_genefamilies.tsv' \
+    -o 'test-data/renamed_genefamilies.tsv' \
+    --names 'metacyc-pwy'
+
+# humann2_split_table
+mkdir split_table_tmp_dir
+humann2_split_table \
+    --input 'test-data/joined_pathway_coverage_abundance.tsv' \
+    -o 'split_table_tmp_dir'
+cp 'split_table_tmp_dir/demo_Abundance.tsv' 'test-data/'
+cp 'split_table_tmp_dir/demo_Coverage.tsv' 'test-data/'