view plotwithscale.xml @ 1:4bd560127b68 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/iwtomics commit 11ee7ac206d41894c0b6a11f2439aaea490824f0

<tool id="iwtomics_plotwithscale" name="IWTomics Plot with Threshold" version="@VERSION@.0">
  <description>on Test Scale</description>
  <macros>
    <import>macros.xml</import>
  </macros>
  <expand macro="requirements" />
  
  <command detect_errors="exit_code">
<![CDATA[
    Rscript '$__tool_directory__/plotwithscale.R' adjustedpvalue='${adjustedpvalue}' iwtomicsrespdf='${iwtomicsrespdf}' iwtomicssumpdf='${iwtomicssumpdf}'
      iwtomicsrdata='${rdata}'
      iwtomicstests='${testids}'
      iwtomicsselectedfeatures='${featureids}'

      #set test_subset = '|'.join( [ str( $e.test ) for $e in $scalesection.thresholdontestscale ] )
      test_subset='${test_subset}'
      #set feature_subset = '|'.join( [ str( $e.feature ) for $e in $scalesection.thresholdontestscale ] )
      feature_subset='${feature_subset}'
      #set scale_subset = ','.join( [ str( $e.scale ) for $e in $scalesection.thresholdontestscale ] )
      scale_subset='c(${scale_subset})'

      testalpha='${plotres.alpha}'
      average='${plotres.average}'
      size='${plotres.size}'
      plottype="'${plotres.conditionalplottype.plottype}'"
      #if $plotres.conditionalplottype.plottype == 'boxplot':
        #set probs = ','.join( [ str( $p.prob ) for $p in $plotres.conditionalplottype.probabilitiessection.probabilities ] )
        #if $probs != "":
          probs='c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2},${probs})'
        #else:
          probs='c(${plotres.conditionalplottype.probabilitiessection.prob0},${plotres.conditionalplottype.probabilitiessection.prob1},${plotres.conditionalplottype.probabilitiessection.prob2})'
        #end if
      #end if

      groupby="'${plotsum.conditionalgroupby.groupby}'"
      #if $plotsum.conditionalgroupby.groupby == "test":
        summaryalpha='${plotsum.conditionalgroupby.testalphaplot}'
        only_significant='${plotsum.conditionalgroupby.testonlysig}'
      #elif $plotsum.conditionalgroupby.groupby == "feature":
        summaryalpha='${plotsum.conditionalgroupby.featurealphaplot}'
        only_significant='${plotsum.conditionalgroupby.featureonlysig}'
      #end if
]]>
  </command>

  <inputs>
    <!-- RData -->
    <param format="rdata" name="rdata" type="data" label="Select IWTomicsData object with test results" help="File created by 'IWTomics Test and Plot'." />
    <!-- test IDs -->
    <param format="tabular" name="testids" type="data" label="Select test IDs" help="File created by 'IWTomics Test and Plot'." />
    <!-- feature IDs -->
    <param format="tabular" name="featureids" type="data" label="Select feature IDs" help="File created by 'IWTomics Test and Plot'." />

    <!-- repeat threshold on test scale -->
    <section name="scalesection" title="Select scale for Interval-Wise Testing and plot" expanded="True" help="Maximum interval length for the p-value adjustment. If 0 the maximum possible scale is used.">
      <repeat name="thresholdontestscale" title="Threshold on test scale" min="1">
        <param name="test" type="data_column" data_ref="testids" numerical="False" label="Test ID" multiple="True" use_header_names="True" />
        <param name="feature" type="data_column" data_ref="featureids" numerical="False" label="Feature ID" multiple="True" use_header_names="True" />
        <param name="scale" type="integer" value="0" min="0" label="Scale" />
      </repeat>
    </section>

    <!-- plot IWTomics results -->
    <section name="plotres" title="Plot IWTomics test results" expanded="True">
      <!-- alpha -->
      <param name="alpha" size="3" type="float" value="0.05" min="0.0" max="1.0" label="Level of the test (alpha)" />
      <expand macro="plot-params" />
    </section>

    <!-- summary plot -->
    <expand macro="plot-sum" />
  </inputs>

  <outputs>
    <data format="txt" name="adjustedpvalue" label="${tool.name} on ${on_string}: Adjusted p-value" from_work_dir="iwtomics.plotwithscale.adjustedpvalue.txt" />
    <data format="pdf" name="iwtomicsrespdf" label="${tool.name} on ${on_string}: Plotted Test Results" from_work_dir="iwtomics.plotwithscale.iwtomicstestresults.pdf" />
    <data format="pdf" name="iwtomicssumpdf" label="${tool.name} on ${on_string}: Summary Plot" from_work_dir="iwtomics.plotwithscale.summaryplot.pdf" />
  </outputs>

  <tests>
    <test>
      <param name="rdata" value="output_testandplot/iwtomics.testandplot.RData" ftype="rdata" />
      <param name="testids" value="output_testandplot/iwtomics.testandplot.tests.txt" ftype="tabular" />
      <param name="featureids" value="output_testandplot/iwtomics.testandplot.selectedfeatures.txt" ftype="tabular" />
      <repeat name="thresholdontestscale">
        <param name="test" value="1" />
        <param name="feature" value="1" />
        <param name="scale" value="10" />
      </repeat>
      <repeat name="thresholdontestscale">
        <param name="test" value="1,2,3" />
        <param name="feature" value="2" />
        <param name="scale" value="20" />
      </repeat>
      <param name="alpha" value="0.05" />
      <param name="average" value="TRUE" />
      <param name="size" value="TRUE" />
      <param name="plottype" value="boxplot" />
      <param name="prob0" value="0.25" />
      <param name="prob1" value="0.5" />
      <param name="prob2" value="0.75" />
      <param name="groupby" value="feature" />
      <param name="featurealphaplot" value="0.05" />
      <param name="featureonlysig" value="TRUE" />
      <output name="adjustedpvalue" file="output_plotwithscale/iwtomics.plotwithscale.adjustedpvalue.txt" compare="sim_size" />
      <output name="iwtomicsrespdf" file="output_plotwithscale/iwtomics.plotwithscale.iwtomicstestresults.pdf" compare="sim_size" />
      <output name="iwtomicssumpdf" file="output_plotwithscale/iwtomics.plotwithscale.summaryplot.pdf" compare="sim_size" />
    </test>
  </tests>

  <help><![CDATA[
This tool allows to select the scale for the Interval-Wise Testing results. In particular, it returns the p-value curves
for the different tests performed at the selected scale, and it creates a graphical representation of the
Interval-Wise Testing results and a summary plot (optional) at the selected scale.

-----

**Input files**

RData file with the IWTomicsData object with test results, tabular files with test IDs and feature IDs.
These files are created by the tool *IWTomics Test and Plot*.

-----

**Output**

The tool returns:

1. TXT file with an adjusted p-value curve for every test performed at the selected scale;
2. PDF file with the plotted test results;
3. PDF file with the summary plot.

-----

.. class:: infomark

**Notes**

This Galaxy tool has been developed by Fabio Cumbo (Third University of Rome, Italy) and Marzia A. Cremona (The Pennsylvania State University, USA).

It implements a simplified version of the function *IWTomicsTest*, *plotTest* and *plotSummary* for *IWTomicsData* objects.
The complete version can be found in the *R/Bioconductor* package *IWTomics* (see vignette_).

.. _vignette: https://bioconductor.org/packages/release/bioc/vignettes/IWTomics/inst/doc/IWTomics.pdf
  ]]></help>

  <expand macro="citations" />

</tool>