view macs2_bdgbroadcall.xml @ 0:fe62ba547975 draft

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<tool id="macs2_bdgbroadcall" name="MACS2 bdgbroadcall" version="@VERSION_STRING@.0">
    <description>Call broad peaks from bedGraph output</description>
    <expand macro="requirements">
        <requirement type="package" version="4.1.0">gnu_awk</requirement>
    </expand>
    <expand macro="version_command" />
    <macros>
        <import>macs2_macros.xml</import>
    </macros>
    <command>
        macs2 bdgbroadcall
            --ifile "${ infile }"
            --cutoff-peak "${ cutoffpeak }"
            --cutoff-link "${ cutofflink }"
            --min-length "${ minlen }"
            --lvl1-max-gap "${ LVL1MAXGAP }"
            --lvl2-max-gap "${ LVL2MAXGAP }"
            --ofile "macs2_bdgbroadcall.bdg"
        &amp;&amp;
        awk '!/^track name/' "macs2_bdgbroadcall.bdg" > "${ outfile }"
    </command>
    <expand macro="stdio" />
    <inputs>
        <param name="infile" type="data" format="bedgraph" label="MACS score in bedGraph" />
        <param name="cutoffpeak" type="float" label="Cutoff for peaks" value="2.0" 
            help="Cutoff for peaks depending on which method you used for score track. If the file contains qvalue scores from MACS2, score 2 means qvalue 0.01. default: 2.0 (--cutoff-peak)"/>
        <param name="cutofflink" type="float" label="Cutoff for peaks" value="1.0" 
            help=" Cutoff for linking regions/low abundance regions depending on which method you used for score track. If the file contains qvalue scores from MACS2, score 1 means qvalue 0.1, and score 0.3 means qvalue 0.5. default: 1 (--cutoff-link)"/>
        <param name="minlen" type="integer" label="Minimum length of peak" value="200" 
            help="It is recommended to set it as d value." />
        <param name="LVL1MAXGAP" type="integer" label="Maximum gap between significant peaks" value="30" 
            help="It is recommended to set it to tag size" />
        <param name="LVL2MAXGAP" type="integer" label="Maximum linking between significant peaks" value="800" 
            help="It is recommended to set it to 4 times of d value" />
    </inputs>
    <outputs>
        <data name="outfile" format="tabular" label="${tool.name} on ${on_string}" />
    </outputs>
    <tests>
        <test>
            <param name="infile" value="callpeak_treatment_part.bdg" ftype="bedgraph" />
            <param name="cutoffpeak" value="2.0"/>
            <param name="cutofflink" value="1.0"/>
            <param name="minlen" value="200"/>
            <param name="LVL1MAXGAP" value="30"/>
            <param name="LVL2MAXGAP" value="800"/>
            <output name="outfile" file="bdgbroadcall_on_callpeak_treatment.tabular"/>
        </test>
    </tests>
    <help>
**What it does**

Call broad peaks from bedGraph output.
bdgbroadcall from macs2

@citation@
    </help>
    <expand macro="citations" />
</tool>