Mercurial > repos > iuc > megahit
diff megahit_wrapper.xml @ 7:a96f3ad4e0d8 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/megahit commit 64421702fce231e2fb05bd2f585679154c3e2140
author | iuc |
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date | Tue, 28 Nov 2023 07:47:49 +0000 |
parents | 14ddfc8ffb93 |
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--- a/megahit_wrapper.xml Sun Jan 09 16:57:44 2022 +0000 +++ b/megahit_wrapper.xml Tue Nov 28 07:47:49 2023 +0000 @@ -1,17 +1,20 @@ -<?xml version='1.0' encoding='utf-8'?> -<tool id="megahit" name="MEGAHIT" version="@TOOL_VERSION@+galaxy0"> +<tool id="megahit" name="MEGAHIT" version="@TOOL_VERSION@+galaxy1"> <description>for metagenomics assembly</description> + <macros> + <token name="@TOOL_VERSION@">1.2.9</token> + </macros> <xrefs> <xref type="bio.tools">megahit</xref> </xrefs> - <macros> - <token name="@TOOL_VERSION@">1.2.9</token> - </macros> <requirements> <requirement type="package" version="@TOOL_VERSION@">megahit</requirement> </requirements> <version_command>megahit --version</version_command> <command detect_errors="exit_code"><![CDATA[ +if [[ -n "\$GALAXY_MEMORY_MB" ]]; then + MEMORY="-m \$((GALAXY_MEMORY_MB * 1024))"; +fi; + megahit --num-cpu-threads \${GALAXY_SLOTS:-4} #if $input_option.choice == 'paired' @@ -52,6 +55,7 @@ ${advanced_section.nolocal} ${advanced_section.kmin1pass} --min-contig-len '${output_section.min_contig_len}' + \$MEMORY && cat megahit_out/log ]]></command> <inputs> @@ -88,36 +92,36 @@ </when> </conditional> <section name="basic_section" title="Basic assembly options" expanded="True"> - <param name="min_count" argument="--min-count" type="integer" value="2" label="minimum multiplicity for filtering (k_min+1)-mers" help="(kmin+1)-mer with multiplicity lower than d (default 2, specified by --min-count option) will be discarded. You should be cautious to set d less than 2, which will lead to a much larger and noisy graph. We recommend using the default value 2 for metagenomics assembly. If you want to use MEGAHIT to do generic assemblies, please change this value according to the sequencing depth. (recommend --min-count 3 for >40x)."/> + <param argument="--min-count" type="integer" value="2" label="minimum multiplicity for filtering (k_min+1)-mers" help="(kmin+1)-mer with multiplicity lower than d (default 2, specified by --min-count option) will be discarded. You should be cautious to set d less than 2, which will lead to a much larger and noisy graph. We recommend using the default value 2 for metagenomics assembly. If you want to use MEGAHIT to do generic assemblies, please change this value according to the sequencing depth. (recommend --min-count 3 for >40x)."/> <conditional name="k_mer"> <param name="k_mer_method" type="select" label="K-mer specification method"> <option value="klist_method">Specify list</option> <option value="klim_method">Specify min, max, and step values</option> </param> <when value="klist_method"> - <param name="k_list" argument="--k-list" type="text" value="21,29,39,59,79,99,119,141" label="Comma-separated list of kmer size" help="all must be odd, in the range 15-255, and with increments <= 28"/> + <param argument="--k-list" type="text" value="21,29,39,59,79,99,119,141" label="Comma-separated list of kmer size" help="all must be odd, in the range 15-255, and with increments <= 28"/> </when> <when value="klim_method"> - <param name="k_min" argument="--k-min" type="integer" value="21" label="Minimum kmer size" max="255" help="Must be odd number. For ultra complex metagenomics data such as soil, a larger kmin, say 27, is recommended to reduce the complexity of the de Bruijn graph. Quality trimming is also recommended. For high-depth generic data, large --k-min (25 to 31) is recommended. Smaller --k-step, say 10, is more friendly to low-coverage datasets."/> - <param name="k_max" argument="--k-max" type="integer" value="141" label="Maximum kmer size" max="255" help="must be odd number"/> - <param name="k_step" argument="--k-step" type="integer" value="12" label="Increment of kmer size of each iteration" max="28" help="must be even number"/> + <param argument="--k-min" type="integer" value="21" label="Minimum kmer size" max="255" help="Must be odd number. For ultra complex metagenomics data such as soil, a larger kmin, say 27, is recommended to reduce the complexity of the de Bruijn graph. Quality trimming is also recommended. For high-depth generic data, large --k-min (25 to 31) is recommended. Smaller --k-step, say 10, is more friendly to low-coverage datasets."/> + <param argument="--k-max" type="integer" value="141" label="Maximum kmer size" max="255" help="must be odd number"/> + <param argument="--k-step" type="integer" value="12" label="Increment of kmer size of each iteration" max="28" help="must be even number"/> </when> </conditional> </section> <section name="advanced_section" title="Advanced assembly options" expanded="False"> <param name="nomercy" type="boolean" checked="false" truevalue="--no-mercy" falsevalue="" label="Do not add mercy kmers" help="Mercy kmers are specially designed for metagenomics assembly to recover low coverage sequences. For generic dataset >= 30x, MEGAHIT may generate better results with no mercy kmers." /> - <param name="bubble_level" argument="--bubble-level" type="integer" value="2" min="0" max="2" label="Intensity of bubble merging (0-2), 0 to disable" /> - <param name="merge_level" argument="--merge-level" type="text" value="20,0.95" label="Merge complex bubbles of length" /> - <param name="prune_level" argument="--prune-level" type="integer" value="2" min="0" max="3" label="Strength of low depth pruning" /> - <param name="prune_depth" argument="--prune-depth" type="integer" value="2" min="0" label="Remove unitigs with avg kmer depth less than this value" /> - <param name="disconnect_ratio" argument="--disconnect-ratio" type="float" value="0.1" label="Disconnect unitigs if its depth is less than this ratio times the total depth of itself and its siblings" /> - <param name="low_local_ratio" argument="--low-local-ratio" type="float" value="0.2" label="Remove unitigs if its depth is less than this ratio times the average depth of the neighborhoods" /> - <param name="cleaning_rounds" argument="--cleaning-rounds" type="integer" value="5" label="Number of rounds for graph cleanning" /> + <param argument="--bubble-level" type="integer" value="2" min="0" max="2" label="Intensity of bubble merging (0-2), 0 to disable" /> + <param argument="--merge-level" type="text" value="20,0.95" label="Merge complex bubbles of length" /> + <param argument="--prune-level" type="integer" value="2" min="0" max="3" label="Strength of low depth pruning" /> + <param argument="--prune-depth" type="integer" value="2" min="0" label="Remove unitigs with avg kmer depth less than this value" /> + <param argument="--disconnect-ratio" type="float" value="0.1" label="Disconnect unitigs if its depth is less than this ratio times the total depth of itself and its siblings" /> + <param argument="--low-local-ratio" type="float" value="0.2" label="Remove unitigs if its depth is less than this ratio times the average depth of the neighborhoods" /> + <param argument="--cleaning-rounds" type="integer" value="5" label="Number of rounds for graph cleanning" /> <param name="nolocal" type="boolean" checked="false" truevalue="--no-local" falsevalue="" label="Disable local assembly" /> <param name="kmin1pass" type="boolean" checked="false" truevalue="--kmin-1pass" falsevalue="" label="Use 1pass mode to build SdBG of k_min" /> </section> <section name="output_section" title="Output options" expanded="True"> - <param name="min_contig_len" argument="--min-contig-len" type="integer" value="200" label="Minimum length of contigs to output" /> + <param argument="--min-contig-len" type="integer" value="200" label="Minimum length of contigs to output" /> <param name="show_intermediate_contigs" type="boolean" checked="false" label="Return intermediate contigs?"/> </section> </inputs> @@ -129,7 +133,7 @@ </collection> </outputs> <tests> - <test> + <test expect_num_outputs="1"> <conditional name="input_option"> <param name="choice" value="single"/> <param name="single_files" value="refExample.fa" ftype="fasta"/> @@ -140,14 +144,14 @@ </assert_contents> </output> </test> - <test> + <test expect_num_outputs="1"> <conditional name="input_option"> <param name="choice" value="interleaved"/> <param name="interleaved_file" value="interleaved-fq.fa"/> </conditional> <output name="output" file="interleaved_result.fa"/> </test> - <test> + <test expect_num_outputs="1"> <conditional name="input_option"> <param name="choice" value="paired"/> <param name="fastq_input1" value="paired-fq1.fa"/> @@ -155,7 +159,7 @@ </conditional> <output name="output" file="paired_result.fa"/> </test> - <test> + <test expect_num_outputs="1"> <conditional name="input_option"> <param name="choice" value="paired_collection"/> <conditional name="batchmode">