view megahit_contig2fastg.xml @ 2:4ec5b3777508 draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/megahit_contig2fastg commit da240ca579196731499169c1bbcd87af46f3921e

<tool id="megahit_contig2fastg" name="megahit contig2fastg" version="@VERSION@+@GALAXY_VERSION@">
    <description>for converting MEGAHIT's contigs (.fa) to assembly graphs (.fastg)</description>
    <macros>
        <token name="@GALAXY_VERSION@">galaxy1</token>
        <token name="@VERSION@">1.1.3</token>
    </macros>
    <requirements>
        <requirement type="package" version="@VERSION@">megahit</requirement>
    </requirements>
    <version_command>megahit --version</version_command>
    <command detect_errors="exit_code"><![CDATA[
        megahit_toolkit contig2fastg
        '${kmer}'
        '${contigs}' > '${fastg}'
    ]]></command>
    <inputs>
        <param name="contigs" type="data" format="fasta" label="Contig file" help="Ex: k99.contigs.fa" />
        <param name="kmer" type="integer" value="99" label="K-mer length" help="Usually indicated within the filename (when derived from MEGAHIT)"/>
    </inputs>
    <outputs>
        <data format="fastg" name="fastg" label="Assembly graph via ${tool.name} on ${on_string}" />
    </outputs>
    <tests>
        <test>
            <param name="contigs" value="k21.contigs.fa" ftype="fasta" />
            <param name="kmer" value="21"/>
            <output name="fastg">
                <assert_contents>
                    <has_text text=">NODE_1_length_576" />
                </assert_contents>
            </output>
        </test>
    </tests>
    <help><![CDATA[

**MEGAHIT toolkit's contig2fastg**

Contig2fastg is a subprogram within the MEGAHIT toolkit. It converts MEGAHIT's contigs (.fa) to assembly graphs (.fastg) that can be utilized for protein/peptide identification via graph2pro and can also be visualized via Bandage.

-----

**EXAMPLE**

*Command:*

megahit_toolkit contig2fastg 99 intermediate_contigs/k99.contigs.fa > k99.contigs.fastg



*k99.contigs.fa*

>k21_1 flag=3 multi=1.0486 len=576

CGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTC...



*k99.contigs.fastg*

>NODE_1_length_576_cov_1.0486_ID_1:NODE_1_length_576_cov_1.0486_ID_1;

CGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTC...

-----

**MEGAHIT**

MEGAHIT is a single node assembler for large and complex metagenomics NGS reads, such as soil. It makes use of succinct de Bruijn graph (SdBG) to achieve low memory assembly. MEGAHIT can optionally utilize a CUDA-enabled GPU to accelerate its SdBG contstruction. The GPU-accelerated version of MEGAHIT has been tested on NVIDIA GTX680 (4G memory) and Tesla K40c (12G memory) with CUDA 5.5, 6.0 and 6.5.

--------

**Project links:**

MEGAHIT and MEGAHIT Toolkit: https://github.com/voutcn/megahit

Graph2Pro-Var: https://github.com/COL-IU/graph2pro-var

Bandage: http://rrwick.github.io/Bandage/


    ]]></help>
    <citations>
        <citation type="doi">10.1093/bioinformatics/btv033</citation>
    </citations>
</tool>