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date | Thu, 17 May 2018 14:11:27 -0400 |
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<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN" "http://www.w3.org/TR/html4/loose.dtd"> <html xmlns:cis="http://zlab.bu.edu/schema/cisml" xmlns:fimo="http://noble.gs.washington.edu/schema/cisml" xmlns:mem="http://noble.gs.washington.edu/meme"> <head> <meta http-equiv="Content-Type" content="text/html; charset=UTF-8"> <meta charset="UTF-8"> <title>FIMO Results</title> <style type="text/css"> td.left {text-align: left;} td.right {text-align: right; padding-right: 1cm;} </style> </head> <body bgcolor="#D5F0FF"> <a name="top_buttons"></a> <hr> <table summary="buttons" align="left" cellspacing="0"> <tr> <td bgcolor="#00FFFF"><a href="#database_and_motifs"><b>Database and Motifs</b></a></td> <td bgcolor="#DDFFDD"><a href="#sec_i"><b>High-scoring Motif Occurences</b></a></td> <td bgcolor="#DDDDFF"><a href="#debugging_information"><b>Debugging Information</b></a></td> </tr> </table> <br/> <br/> <hr/> <center><big><b>FIMO - Motif search tool</b></big></center> <hr> <p> FIMO version 4.12.0, (Release date: Tue Jun 27 16:22:50 2017 -0700) </p> <p> For further information on how to interpret these results or to get a copy of the FIMO software please access <a href="http://meme.nbcr.net">http://meme.nbcr.net</a></p> <p>If you use FIMO in your research, please cite the following paper:<br> Charles E. Grant, Timothy L. Bailey, and William Stafford Noble, "FIMO: Scanning for occurrences of a given motif", <i>Bioinformatics</i>, <b>27</b>(7):1017-1018, 2011. <a href="http://bioinformatics.oxfordjournals.org/content/27/7/1017">[full text]</a></p> <hr> <center><big><b><a name="database_and_motifs">DATABASE AND MOTIFS</a></b></big></center> <hr> <div style="padding-left: 0.75in; line-height: 1em; font-family: monospace;"> <p> DATABASE hsa_chrM.fa <br /> Database contains 1 sequences, 16569 residues </p> <p> MOTIFS dreme_fimo_input_1.xml (DNA) <table> <thead> <tr> <th style="border-bottom: 1px dashed;">MOTIF</th> <th style="border-bottom: 1px dashed; padding-left: 1em;">WIDTH</th> <th style="border-bottom: 1px dashed; padding-left: 1em;text-align:left;" > BEST POSSIBLE MATCH </th> </tr> </thead> <tbody> <tr> <td style="text-align:right;">ACTAAYH</td> <td style="text-align:right;padding-left: 1em;">7</td> <td style="text-align:left;padding-left: 1em;">ACTAACA</td> </tr> <tr> <td style="text-align:right;">YTAACA</td> <td style="text-align:right;padding-left: 1em;">6</td> <td style="text-align:left;padding-left: 1em;">TTAACA</td> </tr> <tr> <td style="text-align:right;">TCTGT</td> <td style="text-align:right;padding-left: 1em;">5</td> <td style="text-align:left;padding-left: 1em;">TCTGT</td> </tr> <tr> <td style="text-align:right;">SCCAGG</td> <td style="text-align:right;padding-left: 1em;">6</td> <td style="text-align:left;padding-left: 1em;">CCCAGG</td> </tr> <tr> <td style="text-align:right;">CCAGCAY</td> <td style="text-align:right;padding-left: 1em;">7</td> <td style="text-align:left;padding-left: 1em;">CCAGCAC</td> </tr> <tr> <td style="text-align:right;">GMATGT</td> <td style="text-align:right;padding-left: 1em;">6</td> <td style="text-align:left;padding-left: 1em;">GAATGT</td> </tr> </tbody> </table> </p> <p> Random model letter frequencies (fimo_background_probs_hsa_chrM.txt): <br/> A 0.278 C 0.222 G 0.222 T 0.278 </p> </div> <hr> <center><big><b><a name="sec_i">SECTION I: HIGH-SCORING MOTIF OCCURENCES</a></b></big></center> <hr> <ul> <li> There were 11 motif occurences with a p-value less than 0.0001. The full set of motif occurences can be seen in the tab-delimited plain text output file <a href="fimo.txt">fimo.txt</a>, the GFF file <a href="fimo.gff">fimo.gff</a> which may be suitable for uploading to the <a href="http://genome.ucsc.edu/cgi-bin/hgTables">UCSC Genome Table Browser</a> (assuming the FASTA input sequences included genomic coordinates in UCSC or Galaxy format), or the XML file <a href="fimo.xml">fimo.xml</a>. </li> <li> The p-value of a motif occurrence is defined as the probability of a random sequence of the same length as the motif matching that position of the sequence with as good or better a score. </li> <li> The score for the match of a position in a sequence to a motif is computed by summing the appropriate entries from each column of the position-dependent scoring matrix that represents the motif. </li> <li> The q-value of a motif occurrence is defined as the false discovery rate if the occurrence is accepted as significant. </li> <li>The table is sorted by increasing p-value.</li> </ul> <table border="1"> <thead> <tr> <th>Motif ID</th> <th>Alt ID</th> <th>Sequence Name</th> <th>Strand</th> <th>Start</th> <th>End</th> <th>p-value</th> <th>q-value</th> <th>Matched Sequence</th> </tr> </thead> <tbody> <tr> <td style="text-align:left;">CCAGCAY</td> <td style="text-align:left;">DREME-5</td> <td style="text-align:left;">chrM</td> <td style="text-align:center;">+</td> <td style="text-align:left;">510</td> <td style="text-align:left;">516</td> <td style="text-align:left;">4.15e-05</td> <td style="text-align:left;">0.668</td> <td style="text-align:left;font-size:x-large;font-family:monospace;">CCAGCAC</td> </tr> <tr> <td style="text-align:left;">CCAGCAY</td> <td style="text-align:left;">DREME-5</td> <td style="text-align:left;">chrM</td> <td style="text-align:center;">+</td> <td style="text-align:left;">5137</td> <td style="text-align:left;">5143</td> <td style="text-align:left;">4.15e-05</td> <td style="text-align:left;">0.668</td> <td style="text-align:left;font-size:x-large;font-family:monospace;">CCAGCAC</td> </tr> <tr> <td style="text-align:left;">ACTAAYH</td> <td style="text-align:left;">DREME-1</td> <td style="text-align:left;">chrM</td> <td style="text-align:center;">+</td> <td style="text-align:left;">440</td> <td style="text-align:left;">446</td> <td style="text-align:left;">8.2e-05</td> <td style="text-align:left;">0.327</td> <td style="text-align:left;font-size:x-large;font-family:monospace;">ACTAACA</td> </tr> <tr> <td style="text-align:left;">ACTAAYH</td> <td style="text-align:left;">DREME-1</td> <td style="text-align:left;">chrM</td> <td style="text-align:center;">-</td> <td style="text-align:left;">2093</td> <td style="text-align:left;">2099</td> <td style="text-align:left;">8.2e-05</td> <td style="text-align:left;">0.327</td> <td style="text-align:left;font-size:x-large;font-family:monospace;">ACTAACA</td> </tr> <tr> <td style="text-align:left;">ACTAAYH</td> <td style="text-align:left;">DREME-1</td> <td style="text-align:left;">chrM</td> <td style="text-align:center;">-</td> <td style="text-align:left;">2299</td> <td style="text-align:left;">2305</td> <td style="text-align:left;">8.2e-05</td> <td style="text-align:left;">0.327</td> <td style="text-align:left;font-size:x-large;font-family:monospace;">ACTAACA</td> </tr> <tr> <td style="text-align:left;">ACTAAYH</td> <td style="text-align:left;">DREME-1</td> <td style="text-align:left;">chrM</td> <td style="text-align:center;">+</td> <td style="text-align:left;">5186</td> <td style="text-align:left;">5192</td> <td style="text-align:left;">8.2e-05</td> <td style="text-align:left;">0.327</td> <td style="text-align:left;font-size:x-large;font-family:monospace;">ACTAACA</td> </tr> <tr> <td style="text-align:left;">ACTAAYH</td> <td style="text-align:left;">DREME-1</td> <td style="text-align:left;">chrM</td> <td style="text-align:center;">+</td> <td style="text-align:left;">6530</td> <td style="text-align:left;">6536</td> <td style="text-align:left;">8.2e-05</td> <td style="text-align:left;">0.327</td> <td style="text-align:left;font-size:x-large;font-family:monospace;">ACTAACA</td> </tr> <tr> <td style="text-align:left;">ACTAAYH</td> <td style="text-align:left;">DREME-1</td> <td style="text-align:left;">chrM</td> <td style="text-align:center;">+</td> <td style="text-align:left;">7742</td> <td style="text-align:left;">7748</td> <td style="text-align:left;">8.2e-05</td> <td style="text-align:left;">0.327</td> <td style="text-align:left;font-size:x-large;font-family:monospace;">ACTAACA</td> </tr> <tr> <td style="text-align:left;">ACTAAYH</td> <td style="text-align:left;">DREME-1</td> <td style="text-align:left;">chrM</td> <td style="text-align:center;">+</td> <td style="text-align:left;">13657</td> <td style="text-align:left;">13663</td> <td style="text-align:left;">8.2e-05</td> <td style="text-align:left;">0.327</td> <td style="text-align:left;font-size:x-large;font-family:monospace;">ACTAACA</td> </tr> <tr> <td style="text-align:left;">ACTAAYH</td> <td style="text-align:left;">DREME-1</td> <td style="text-align:left;">chrM</td> <td style="text-align:center;">+</td> <td style="text-align:left;">13741</td> <td style="text-align:left;">13747</td> <td style="text-align:left;">8.2e-05</td> <td style="text-align:left;">0.327</td> <td style="text-align:left;font-size:x-large;font-family:monospace;">ACTAACA</td> </tr> <tr> <td style="text-align:left;">CCAGCAY</td> <td style="text-align:left;">DREME-5</td> <td style="text-align:left;">chrM</td> <td style="text-align:center;">+</td> <td style="text-align:left;">4241</td> <td style="text-align:left;">4247</td> <td style="text-align:left;">9.37e-05</td> <td style="text-align:left;">1</td> <td style="text-align:left;font-size:x-large;font-family:monospace;">CCAGCAT</td> </tr> </tbody> </table> <hr> <center><big><b><a name="debugging_information">DEBUGGING INFORMATION</a></b></big></center> <hr> <p> Command line: </p> <pre> fimo -oc fimo_test2_out --bgfile fimo_background_probs_hsa_chrM.txt dreme_fimo_input_1.xml hsa_chrM.fa </pre> <p> Settings: </p> <pre> <table> <tr> <td style="padding-right: 2em">output_directory = fimo_test2_out</td> <td style="padding-left: 5em; padding-right: 2em">MEME file name = dreme_fimo_input_1.xml</td> <td style="padding-left: 5em; padding-right: 2em">sequence file name = hsa_chrM.fa</td> </tr> <tr> <td style="padding-right: 2em">background file name = fimo_background_probs_hsa_chrM.txt</td> <td style="padding-left: 5em; padding-right: 2em">alphabet = DNA</td> <td style="padding-left: 5em; padding-right: 2em">max stored scores = 100000</td> </tr> <tr> <td style="padding-right: 2em">allow clobber = true</td> <td style="padding-left: 5em; padding-right: 2em">compute q-values = true</td> <td style="padding-left: 5em; padding-right: 2em">parse genomic coord. = false</td> </tr> <tr> <td style="padding-right: 2em">text only = false</td> <td style="padding-left: 5em; padding-right: 2em">scan both strands = true</td> <td style="padding-left: 5em; padding-right: 2em">max strand = false</td> </tr> <tr> <td style="padding-right: 2em">threshold type = p-value</td> <td style="padding-left: 5em; padding-right: 2em">output theshold = 0.0001</td> <td style="padding-left: 5em; padding-right: 2em">pseudocount = 0.1</td> </tr> <tr> <td style="padding-right: 2em">alpha = 1</td> <td style="padding-left: 5em; padding-right: 2em">verbosity = 2</td> <td style="padding-left: 5em; padding-right: 2em"></td> </tr> </table> </pre> <p> This information can be useful in the event you wish to report a problem with the FIMO software. </p> <hr> <span style="background-color: #DDDDFF"><a href="#top_buttons"><b>Go to top</b></a></span> </body> </html>