Mercurial > repos > iuc > meme_fimo
changeset 5:eca84de658b0 draft
Uploaded
author | iuc |
---|---|
date | Fri, 17 Jun 2016 13:15:48 -0400 |
parents | cd54079f0f72 |
children | cdcc4bb60bc3 |
files | fimo.xml fimo_wrapper.py test-data/fimo_output_html_1.html test-data/fimo_output_html_2.html test-data/fimo_output_xml_1.xml test-data/fimo_output_xml_2.xml test-data/meme_input_1.fasta test-data/meme_output_html_1.html test-data/meme_output_html_2.html test-data/meme_output_txt_1.txt test-data/meme_output_txt_2.txt test-data/motif1.gff test-data/prior30.plib tool_dependencies.xml |
diffstat | 14 files changed, 230 insertions(+), 393 deletions(-) [+] |
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--- a/fimo.xml Tue Mar 08 08:10:52 2016 -0500 +++ b/fimo.xml Fri Jun 17 13:15:48 2016 -0400 @@ -1,4 +1,4 @@ -<tool id="meme_fimo" name="FIMO" version="4.11.0.2"> +<tool id="meme_fimo" name="FIMO" version="4.11.0.3"> <description>- Scan a set of sequences for motifs.</description> <requirements> <requirement type="package" version="6.9.3">imagemagick</requirement> @@ -6,6 +6,7 @@ </requirements> <command> <![CDATA[ + mkdir -p output && python $__tool_directory__/fimo_wrapper.py --input_motifs "${input_motifs}" #if str($fasta_type.fasta_type_selector) == 'history': @@ -28,10 +29,14 @@ --motif "${motif.motif}" #end for #end if + --output_separate_motifs ${options_type.output_separate_motifs} --motif_pseudo "${options_type.motif_pseudo}" ${options_type.no_qvalue} ${options_type.norc} - ${options_type.parse_genomic_coord} + #if str($options_type.parse_genomic_coord_cond.parse_genomic_coord) == 'yes': + --parse_genomic_coord 'yes' + --remove_duplicate_coords ${options_type.parse_genomic_coord_cond.remove_duplicate_coords} + #end if #if str($options_type.psp_cond.psp_selector) == 'yes': --input_psp "${input_psp}" #end if @@ -99,10 +104,23 @@ </repeat> </when> </conditional> + <param name="output_separate_motifs" type="select" label="Output a dataset per motif?" help="Output a collection consisting of a separate dataset for each motif in the input"> + <option value="no" selected="true">No</option> + <option value="yes">Yes</option> + </param> <param name="motif_pseudo" type="float" value="0.1" label="Pseudocount to add to counts in motif matrix" help="A pseudocount to be added to each count in the motif matrix, after first multiplying by the corresponding background frequency"/> <param name="no_qvalue" label="Do not compute a q-value for each p-value" type="boolean" truevalue="--no_qvalue" falsevalue="" checked="True" help="The q-value calculation is that of Benjamini and Hochberg (1995)."/> <param name="norc" label="Do not score the reverse complement DNA strand" type="boolean" truevalue="--norc" falsevalue="" checked="False" /> - <param name="parse_genomic_coord" label="Check each sequence header for UCSC style genomic coordinates" type="boolean" truevalue="--parse_genomic_coord" falsevalue="" checked="False" /> + <conditional name="parse_genomic_coord_cond"> + <param name="parse_genomic_coord" label="Check each sequence header for UCSC style genomic coordinates" type="select"> + <option value="no" selected="true">No</option> + <option value="yes">Yes</option> + </param> + <when value="yes"> + <param name="remove_duplicate_coords" type="boolean" truevalue="yes" falsevalue="no" label="Remove duplicate entries in unique GFF coordinates?" help="Remove duplicate entries as defined by the unique GFF coordinates" /> + </when> + <when value="no"/> + </conditional> <conditional name="psp_cond"> <param name="psp_selector" type="select" label="Use position-specific priors?"> <option value="no" selected="true">No</option> @@ -160,6 +178,7 @@ </actions> </data> <data format="tabular" name="gff_outfile" label="${tool.name} on ${on_string} (almost-gff)"> + <filter>options_type['output_separate_motifs'] == 'no'</filter> <actions> <conditional name="fasta_type.fasta_type_selector"> <when value="cached"> @@ -172,6 +191,10 @@ </conditional> </actions> </data> + <collection name="motifs" type="list" label="Motifs: ${tool.name} on ${on_string}"> + <discover_datasets pattern="(?P<designation>.*)" directory="output" ext="gff" visible="false" /> + <filter>options_type['output_separate_motifs'] == 'yes'</filter> + </collection> <data format="cisml" name="xml_outfile" label="${tool.name} on ${on_string} (xml)"> <actions> <conditional name="fasta_type.fasta_type_selector"> @@ -209,7 +232,7 @@ <output name="html_outfile" file="fimo_output_html_1.html" compare="contains"/> <output name="txt_outfile" file="fimo_output_txt_1.txt" compare="contains"/> <output name="gff_outfile" file="fimo_output_almost-gff_1.txt" compare="contains"/> - <output name="xml_outfile" file="fimo_output_xml_1.xml" lines_diff="8"/> + <output name="xml_outfile" file="fimo_output_xml_1.xml" compare="contains"/> <output name="interval_outfile" file="fimo_output_interval_1.txt" compare="contains"/> </test> <test> @@ -221,7 +244,24 @@ <output name="html_outfile" file="fimo_output_html_2.html" compare="contains"/> <output name="txt_outfile" file="fimo_output_txt_2.txt" compare="contains"/> <output name="gff_outfile" file="fimo_output_almost-gff_2.txt" compare="contains"/> - <output name="xml_outfile" file="fimo_output_xml_2.xml" lines_diff="8"/> + <output name="xml_outfile" file="fimo_output_xml_2.xml" compare="contains"/> + <output name="interval_outfile" file="fimo_output_interval_2.txt" compare="contains"/> + </test> + <test> + <param name="input_motifs" value="meme_output_xml_1.xml" ftype="memexml"/> + <param name="fasta_type_selector" value="history"/> + <param name="input_database" value="phiX.fasta" ftype="fasta"/> + <param name="options_type_selector" value="advanced"/> + <param name="parse_genomic_coord" value="--parse_genomic_coord"/> + <param name="remove_duplicate_coords" value="yes"/> + <param name="output_separate_motifs" value="yes"/> + <param name="non_commercial_use" value="True"/> + <output name="html_outfile" file="fimo_output_html_2.html" compare="contains"/> + <output name="txt_outfile" file="fimo_output_txt_2.txt" compare="contains"/> + <output_collection name="motifs" type="list"> + <element name="MOTIF1.gff" file="motif1.gff" ftype="gff" compare="contains"/> + </output_collection> + <output name="xml_outfile" file="fimo_output_xml_2.xml" compare="contains"/> <output name="interval_outfile" file="fimo_output_interval_2.txt" compare="contains"/> </test> </tests> @@ -235,7 +275,7 @@ FIMO scans a sequence database for individual matches to each of the motifs you provide (sample output for motifs and sequences). The name FIMO stands for 'Find Individual Motif Occurrences'. The program searches a database of sequences for occurrences of known motifs, treating each motif independently. Motifs must be in MEME Motif Format. You can define the statistical threshold -(p-value) for motifs and whether FIMO scans just the given sequences or their reverse complements (where applicable), too. +(p-value) for motifs and whether FIMO scans just the given sequences or their reverse complements (where applicable). .. class:: infomark
--- a/fimo_wrapper.py Tue Mar 08 08:10:52 2016 -0500 +++ b/fimo_wrapper.py Fri Jun 17 13:15:48 2016 -0400 @@ -12,6 +12,19 @@ DNA_COMPLEMENT = string.maketrans("ACGTRYKMBDHVacgtrykmbdhv", "TGCAYRMKVHDBtgcayrmkvhdb") +def get_stderr(tmp_stderr): + tmp_stderr.seek(0) + stderr = '' + try: + while True: + stderr += tmp_stderr.read(BUFFSIZE) + if not stderr or len(stderr) % BUFFSIZE != 0: + break + except OverflowError: + pass + return stderr + + def reverse(sequence): # Reverse sequence string. return sequence[::-1] @@ -43,11 +56,13 @@ parser.add_argument('--max_strand', action='store_true', help='If matches on both strands at a given position satisfy the output threshold, only report the match for the strand with the higher score') parser.add_argument('--max_stored_scores', dest='max_stored_scores', type=int, help='Maximum score count to store') parser.add_argument('--motif', dest='motifs', action='append', default=[], help='Specify motif by id') +parser.add_argument('--output_separate_motifs', default="no", help='Output one dataset per motif') parser.add_argument('--motif_pseudo', dest='motif_pseudo', type=float, default=0.1, help='Pseudocount to add to counts in motif matrix') parser.add_argument('--no_qvalue', action='store_true', help='Do not compute a q-value for each p-value') parser.add_argument('--norc', action='store_true', help='Do not score the reverse complement DNA strand') parser.add_argument('--output_path', dest='output_path', help='Output files directory') -parser.add_argument('--parse_genomic_coord', action='store_true', help='Check each sequence header for UCSC style genomic coordinates') +parser.add_argument('--parse_genomic_coord', default='no', help='Check each sequence header for UCSC style genomic coordinates') +parser.add_argument('--remove_duplicate_coords', default='no', help='Remove duplicate entries in unique GFF coordinates') parser.add_argument('--qv_thresh', action='store_true', help='Use q-values for the output threshold') parser.add_argument('--thresh', dest='thresh', type=float, help='p-value threshold') parser.add_argument('--gff_output', dest='gff_output', help='Gff output file') @@ -73,7 +88,7 @@ fimo_cmd_list.append('--no-qvalue') if args.norc: fimo_cmd_list.append('--norc') - if args.parse_genomic_coord: + if args.parse_genomic_coord == 'yes': fimo_cmd_list.append('--parse-genomic-coord') if args.qv_thresh: fimo_cmd_list.append('--qv-thresh') @@ -93,22 +108,66 @@ tmp_stderr = tempfile.NamedTemporaryFile() proc = subprocess.Popen(args=fimo_cmd, shell=True, stderr=tmp_stderr) returncode = proc.wait() - tmp_stderr.seek(0) - stderr = '' - try: - while True: - stderr += tmp_stderr.read(BUFFSIZE) - if not stderr or len(stderr) % BUFFSIZE != 0: - break - except OverflowError: - pass if returncode != 0: + stderr = get_stderr(tmp_stderr) stop_err(stderr) except Exception, e: stop_err('Error running FIMO:\n%s' % str(e)) shutil.move(os.path.join(args.output_path, 'fimo.txt'), args.txt_output) -shutil.move(os.path.join(args.output_path, 'fimo.gff'), args.gff_output) + +gff_file = os.path.join(args.output_path, 'fimo.gff') +if args.remove_duplicate_coords == 'yes': + tmp_stderr = tempfile.NamedTemporaryFile() + # Identify and eliminating identical motif occurrences. These + # are identical if the combination of chrom, start, end and + # motif id are identical. + cmd = 'sort -k1,1 -k4,4n -k5,5n -k9.1,9.6 -u -o %s %s' % (gff_file, gff_file) + proc = subprocess.Popen(args=cmd, stderr=tmp_stderr, shell=True) + returncode = proc.wait() + if returncode != 0: + stderr = get_stderr(tmp_stderr) + stop_err(stderr) + # Sort GFF output by a combination of chrom, score, start. + cmd = 'sort -k1,1 -k4,4n -k6,6n -o %s %s' % (gff_file, gff_file) + proc = subprocess.Popen(args=cmd, stderr=tmp_stderr, shell=True) + returncode = proc.wait() + if returncode != 0: + stderr = get_stderr(tmp_stderr) + stop_err(stderr) +if args.output_separate_motifs == 'yes': + # Create the collection output directory. + collection_path = (os.path.join(os.getcwd(), 'output')) + # Keep track of motif occurrences. + header_line = None + motif_ids = [] + file_handles = [] + for line in open(gff_file, 'r'): + if line.startswith('#'): + if header_line is None: + header_line = line + continue + items = line.split('\t') + attribute = items[8] + attributes = attribute.split(';') + name = attributes[0] + motif_id = name.split('=')[1] + file_name = os.path.join(collection_path, 'MOTIF%s.gff' % motif_id) + if motif_id in motif_ids: + i = motif_ids.index(motif_id) + fh = file_handles[i] + fh.write(line) + else: + fh = open(file_name, 'wb') + if header_line is not None: + fh.write(header_line) + fh.write(line) + motif_ids.append(motif_id) + file_handles.append(fh) + for file_handle in file_handles: + file_handle.close() +else: + shutil.move(gff_file, args.gff_output) shutil.move(os.path.join(args.output_path, 'fimo.xml'), args.xml_output) shutil.move(os.path.join(args.output_path, 'fimo.html'), args.html_output)
--- a/test-data/fimo_output_html_1.html Tue Mar 08 08:10:52 2016 -0500 +++ b/test-data/fimo_output_html_1.html Fri Jun 17 13:15:48 2016 -0400 @@ -41,12 +41,10 @@ <hr> <div style="padding-left: 0.75in; line-height: 1em; font-family: monospace;"> <p> - DATABASE /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2541.dat <br /> Database contains 1 sequences, 5386 residues </p> <p> - MOTIFS /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2540.dat (Protein) <table> <thead> <tr> @@ -98,3 +96,5 @@ probability of a random sequence of the same length as the motif matching that position of the sequence with as good or better a score. </li> +<li> +The score for the match of a position in a sequence to a motif
--- a/test-data/fimo_output_html_2.html Tue Mar 08 08:10:52 2016 -0500 +++ b/test-data/fimo_output_html_2.html Fri Jun 17 13:15:48 2016 -0400 @@ -41,12 +41,10 @@ <hr> <div style="padding-left: 0.75in; line-height: 1em; font-family: monospace;"> <p> - DATABASE /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2541.dat <br /> Database contains 1 sequences, 5386 residues </p> <p> - MOTIFS /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2540.dat (Protein) <table> <thead> <tr> @@ -98,3 +96,5 @@ probability of a random sequence of the same length as the motif matching that position of the sequence with as good or better a score. </li> +<li> +The score for the match of a position in a sequence to a motif
--- a/test-data/fimo_output_xml_1.xml Tue Mar 08 08:10:52 2016 -0500 +++ b/test-data/fimo_output_xml_1.xml Fri Jun 17 13:15:48 2016 -0400 @@ -4,11 +4,7 @@ xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation= xmlns:fimo="http://noble.gs.washington.edu/schema/fimo" > -<command-line>fimo --o /Users/gvk/work/git_workspace/galaxy/database/job_working_directory/001/1973/dataset_2713_files --verbosity 1 /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2540.dat /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2541.dat</command-line> <settings> -<setting name="output directory">/Users/gvk/work/git_workspace/galaxy/database/job_working_directory/001/1973/dataset_2713_files</setting> -<setting name="MEME file name">/Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2540.dat</setting> -<setting name="sequence file name">/Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2541.dat</setting> <setting name="allow clobber">false</setting> <setting name="compute q-values">true</setting> <setting name="parse genomic coord.">false</setting>
--- a/test-data/fimo_output_xml_2.xml Tue Mar 08 08:10:52 2016 -0500 +++ b/test-data/fimo_output_xml_2.xml Fri Jun 17 13:15:48 2016 -0400 @@ -4,14 +4,9 @@ xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation= xmlns:fimo="http://noble.gs.washington.edu/schema/fimo" > -<command-line>fimo --alpha 1.000000 --max-stored-scores 100000 --motif-pseudo 0.100000 --no-qvalue --thresh 0.000100 --o /Users/gvk/work/git_workspace/galaxy/database/job_working_directory/001/1978/dataset_2738_files --verbosity 1 /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2540.dat /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2541.dat</command-line> <settings> -<setting name="output directory">/Users/gvk/work/git_workspace/galaxy/database/job_working_directory/001/1978/dataset_2738_files</setting> -<setting name="MEME file name">/Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2540.dat</setting> -<setting name="sequence file name">/Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2541.dat</setting> <setting name="allow clobber">false</setting> <setting name="compute q-values">false</setting> -<setting name="parse genomic coord.">false</setting> <setting name="text only">false</setting> <setting name="scan both strands">false</setting> <setting name="output threshold">0.0001</setting>
--- a/test-data/meme_input_1.fasta Tue Mar 08 08:10:52 2016 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,66 +0,0 @@ ->chr21_19617074_19617124_+ -AAAAATTATTACTAGGGAGGGGGCCGGAACCTCGGGACGTGGGTATATAA ->chr21_26934381_26934431_+ -GCGCCTGGTCGGTTATGAGTCACAAGTGAGTTATAAAAGGGTCGCACGTT ->chr21_28217753_28217803_- -CAAAGGGGAGGAGTGGGGTGGGGGTGGGGGTTTCACTGGTCCACTATAAA ->chr21_31710037_31710087_- -AACACCCAGGTTTCTGAGTATATAATCGCCGCACCAAAGAATTTAATTTT ->chr21_31744582_31744632_- -CCCAGGTCTAAGAGCATATATAACTTGGAGTCCAGACTATGACATTCAAA ->chr21_31768316_31768366_+ -AACGTATATAAATGGTCCTGTCCAGATGTGGCATGCAAACTCAGAATCTT ->chr21_31914206_31914256_- -TGACACCCACTACTTAGAGTATAAAATCATTCTGAGAAGTTAGAGACACC ->chr21_31933633_31933683_- -TCAGAGTATATATAAATGTTCCTGTCCAGTCACAGTCACCAAACTGACCT ->chr21_31962741_31962791_- -ACATATAACTCAGGTTGGATAAAATAATTTGTACAAATCAGGAGAGTCAA ->chr21_31964683_31964733_+ -TCTGATTCACTGAGGCATATAAAAGGCCCTCTGCGGAGAAGTGTCCATAC ->chr21_31973364_31973414_+ -aaacttaaaactctataaacttaaaactCTAGAATCTGATCCTGCTATAC ->chr21_31992870_31992920_+ -CTCATACACTATTGAAGATGTATAAAATTTCATTTGCAGATGGTGACATT ->chr21_32185595_32185645_- -TCACCACCCACCAGAGCTGGGATATATAAAGAAGGTTCTGAGACTAGGAA ->chr21_32202076_32202126_- -TGCCCACCAGCTTGAGGTATAAAAAGCCCTGTACGGGAAGAGACCTTCAT ->chr21_32253899_32253949_- -AGCCCCACCCACCAGCAAGGATATATAAAAGCTCAGGAGTCTGGAGTGAC ->chr21_32410820_32410870_- -TCTACCCCACTAATCACTGAGGATGTATAAAAGTCCCAGGGAAGCTGGTG ->chr21_36411748_36411798_- -ATAGTTCTGTATAGTTTCAGTTGGCATCtaaaaattatataactttattt ->chr21_37838750_37838800_- -gatggttttataaggggcctcaccctcggctcagccctcattcttctcct ->chr21_45705687_45705737_+ -CCGGGGCGGAGCGGCCTTTGCTCTTTGCGTGGTCGCGGGGGTATAACAGC ->chr21_45971413_45971463_- -CAGGCCCTGGGCATATAAAAGCCCCAGCAGCCAACAGGctcacacacaca ->chr21_45978668_45978718_- -CAGAGGGGTATAAAGGTTCCGACCACTCAGAGGCCTGGCACGAtcactca ->chr21_45993530_45993580_+ -CCAAGGAGGAGTATAAAAGCCCCACAAACCCGAGCACCTCACTCACTCGC ->chr21_46020421_46020471_+ -GAGACATATAAAAGCCAACATCCCTGAGCACCTAACACACGGactcactc ->chr21_46031920_46031970_+ -GGAAAATACCCAGGGAGGGTATAAAACCTCAGCAGCCAGGGCACACAAAC ->chr21_46046964_46047014_+ -ACAAGGCCAGGAGGGGTATAAAAGCCTGAGAGCCCCAAGAACctcacaca ->chr21_46057197_46057247_+ -ATTGCTGAGTCTCCTGCTGGGAAAACACAGGCCCTGGGCATATAAAAGCC ->chr21_46086869_46086919_- -GACAGGTGTGCTTCTGTGCTGTGGGGATGCCTGGGCCCAGGTATAAAGGC ->chr21_46102103_46102153_- -AGGTGTGTGCTTCTGTGCTGTGGGGATGCCTGGGTCCAGGTATAAAGGCT ->chr21_47517957_47518007_+ -CCTGGCGGCGGGGCGGGTCAGGCCGGCGGGGCGGGGTATAAAGGGGGCGG ->chr21_47517957_47518007_+ -CCTGGCGGCGGGGCGGGTCAGGCCGGCGGGGCGGGGTATAAAGGGGGCGG ->chr21_47517957_47518007_+ -CCTGGCGGCGGGGCGGGTCAGGCCGGCGGGGCGGGGTATAAAGGGGGCGG ->chr21_47575506_47575556_- -TGAGAAGCCGGTGGGGAGGTGCTGCCGGTGAGCGTATAAAGGCCCTGGCG ->chr21_47575506_47575556_- -TGAGAAGCCGGTGGGGAGGTGCTGCCGGTGAGCGTATAAAGGCCCTGGCG
--- a/test-data/meme_output_html_1.html Tue Mar 08 08:10:52 2016 -0500 +++ b/test-data/meme_output_html_1.html Fri Jun 17 13:15:48 2016 -0400 @@ -12,9 +12,6 @@ "stop_reason": "Stopped because requested number of motifs (1) found.", "cmd": [ "meme", - "/Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2490.dat", - "-o", - "/Users/gvk/work/git_workspace/galaxy/database/job_working_directory/001/1912/dataset_2530_files", "-nostatus" ], "options": {
--- a/test-data/meme_output_html_2.html Tue Mar 08 08:10:52 2016 -0500 +++ b/test-data/meme_output_html_2.html Fri Jun 17 13:15:48 2016 -0400 @@ -12,15 +12,6 @@ "stop_reason": "Stopped because requested number of motifs (1) found.", "cmd": [ "meme", - "/Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2490.dat", - "-o", - "/Users/gvk/work/git_workspace/galaxy/database/job_working_directory/001/1929/dataset_2578_files", - "-nostatus", "-sf", "Galaxy_FASTA_Input", "-dna", "-mod", "zoops", - "-nmotifs", "1", "-wnsites", "0.8", "-minw", "8", "-maxw", "50", - "-wg", "11", "-ws", "1", "-maxiter", "50", "-distance", "0.001", - "-prior", "dirichlet", "-b", "0.01", "-plib", - "/Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2577.dat", - "-spmap", "uni", "-spfuzz", "0.5" ], "options": { "mod": "zoops",
--- a/test-data/meme_output_txt_1.txt Tue Mar 08 08:10:52 2016 -0500 +++ b/test-data/meme_output_txt_1.txt Fri Jun 17 13:15:48 2016 -0400 @@ -28,7 +28,7 @@ ******************************************************************************** TRAINING SET ******************************************************************************** -DATAFILE= /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2490.dat +DATAFILE= ALPHABET= ACDEFGHIKLMNPQRSTVWY Sequence name Weight Length Sequence name Weight Length ------------- ------ ------ ------------- ------ ------ @@ -55,7 +55,7 @@ This information can also be useful in the event you wish to report a problem with the MEME software. -command: meme /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2490.dat -o /Users/gvk/work/git_workspace/galaxy/database/job_working_directory/001/1912/dataset_2530_files -nostatus +command: meme model: mod= zoops nmotifs= 1 evt= inf object function= E-value of product of p-values @@ -267,7 +267,7 @@ -Time 0.53 secs. +Time ******************************************************************************** @@ -320,6 +320,6 @@ Stopped because requested number of motifs (1) found. ******************************************************************************** -CPU: MacBook-Pro-2.local +CPU: ********************************************************************************
--- a/test-data/meme_output_txt_2.txt Tue Mar 08 08:10:52 2016 -0500 +++ b/test-data/meme_output_txt_2.txt Fri Jun 17 13:15:48 2016 -0400 @@ -55,7 +55,7 @@ This information can also be useful in the event you wish to report a problem with the MEME software. -command: meme /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2490.dat -o /Users/gvk/work/git_workspace/galaxy/database/job_working_directory/001/1929/dataset_2578_files -nostatus -sf Galaxy_FASTA_Input -dna -mod zoops -nmotifs 1 -wnsites 0.8 -minw 8 -maxw 50 -wg 11 -ws 1 -maxiter 50 -distance 0.001 -prior dirichlet -b 0.01 -plib /Users/gvk/work/git_workspace/galaxy/database/files/002/dataset_2577.dat -spmap uni -spfuzz 0.5 +command: meme model: mod= zoops nmotifs= 1 evt= inf object function= E-value of product of p-values @@ -69,7 +69,7 @@ data: n= 1500 N= 30 shuffle= -1 strands: + sample: seed= 0 ctfrac= -1 maxwords= -1 -Dirichlet mixture priors file: dataset_2577.dat +Dirichlet mixture priors file: Letter frequencies in dataset: A 0.294 C 0.231 G 0.257 T 0.217 Background letter frequencies (from dataset with add-one prior applied): @@ -261,7 +261,7 @@ -Time 0.17 secs. +Time ******************************************************************************** @@ -314,6 +314,6 @@ Stopped because requested number of motifs (1) found. ******************************************************************************** -CPU: dot1x-cb-51.aset.psu.edu +CPU: ********************************************************************************
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/motif1.gff Fri Jun 17 13:15:48 2016 -0400 @@ -0,0 +1,100 @@ +##gff-version 3 +phiX174 fimo polypeptide_motif 1 11 78.3 + . Name=1;ID=1-57-phiX174;pvalue=1.46e-08;sequence=GAGTTTTATCG; +phiX174 fimo polypeptide_motif 3 13 57.5 + . Name=1;ID=1-471-phiX174;pvalue=1.79e-06;sequence=GTTTTATCGCT; +phiX174 fimo polypeptide_motif 7 17 45 + . Name=1;ID=1-1378-phiX174;pvalue=3.18e-05;sequence=TATCGCTTCCA; +phiX174 fimo polypeptide_motif 10 20 53.9 + . Name=1;ID=1-605-phiX174;pvalue=4.1e-06;sequence=CGCTTCCATGA; +phiX174 fimo polypeptide_motif 17 27 40.2 + . Name=1;ID=1-1887-phiX174;pvalue=9.55e-05;sequence=ATGACGCAGAA; +phiX174 fimo polypeptide_motif 18 28 45.3 + . Name=1;ID=1-1349-phiX174;pvalue=2.98e-05;sequence=TGACGCAGAAG; +phiX174 fimo polypeptide_motif 19 29 55.8 + . Name=1;ID=1-527-phiX174;pvalue=2.6e-06;sequence=GACGCAGAAGT; +phiX174 fimo polypeptide_motif 21 31 41.5 + . Name=1;ID=1-1705-phiX174;pvalue=7.07e-05;sequence=CGCAGAAGTTA; +phiX174 fimo polypeptide_motif 22 32 44.6 + . Name=1;ID=1-1404-phiX174;pvalue=3.44e-05;sequence=GCAGAAGTTAA; +phiX174 fimo polypeptide_motif 24 34 79.1 + . Name=1;ID=1-53-phiX174;pvalue=1.23e-08;sequence=AGAAGTTAACA; +phiX174 fimo polypeptide_motif 25 35 45.3 + . Name=1;ID=1-1347-phiX174;pvalue=2.97e-05;sequence=GAAGTTAACAC; +phiX174 fimo polypeptide_motif 26 36 59.2 + . Name=1;ID=1-417-phiX174;pvalue=1.19e-06;sequence=AAGTTAACACT; +phiX174 fimo polypeptide_motif 30 40 44.7 + . Name=1;ID=1-1399-phiX174;pvalue=3.4e-05;sequence=TAACACTTTCG; +phiX174 fimo polypeptide_motif 37 47 72.4 + . Name=1;ID=1-98-phiX174;pvalue=5.79e-08;sequence=TTCGGATATTT; +phiX174 fimo polypeptide_motif 39 49 65.3 + . Name=1;ID=1-213-phiX174;pvalue=2.92e-07;sequence=CGGATATTTCT; +phiX174 fimo polypeptide_motif 41 51 55.3 + . Name=1;ID=1-548-phiX174;pvalue=2.97e-06;sequence=GATATTTCTGA; +phiX174 fimo polypeptide_motif 43 53 58.4 + . Name=1;ID=1-442-phiX174;pvalue=1.43e-06;sequence=TATTTCTGATG; +phiX174 fimo polypeptide_motif 46 56 53.7 + . Name=1;ID=1-617-phiX174;pvalue=4.23e-06;sequence=TTCTGATGAGT; +phiX174 fimo polypeptide_motif 50 60 45.4 + . Name=1;ID=1-1333-phiX174;pvalue=2.86e-05;sequence=GATGAGTCGAA; +phiX174 fimo polypeptide_motif 51 61 48.4 + . Name=1;ID=1-1094-phiX174;pvalue=1.44e-05;sequence=ATGAGTCGAAA; +phiX174 fimo polypeptide_motif 52 62 83.9 + . Name=1;ID=1-22-phiX174;pvalue=4.06e-09;sequence=TGAGTCGAAAA; +phiX174 fimo polypeptide_motif 53 63 53.9 + . Name=1;ID=1-601-phiX174;pvalue=4.03e-06;sequence=GAGTCGAAAAA; +phiX174 fimo polypeptide_motif 54 64 62.9 + . Name=1;ID=1-297-phiX174;pvalue=5.16e-07;sequence=AGTCGAAAAAT; +phiX174 fimo polypeptide_motif 55 65 52.8 + . Name=1;ID=1-675-phiX174;pvalue=5.26e-06;sequence=GTCGAAAAATT; +phiX174 fimo polypeptide_motif 56 66 41.4 + . Name=1;ID=1-1713-phiX174;pvalue=7.2e-05;sequence=TCGAAAAATTA; +phiX174 fimo polypeptide_motif 58 68 43.4 + . Name=1;ID=1-1500-phiX174;pvalue=4.56e-05;sequence=GAAAAATTATC; +phiX174 fimo polypeptide_motif 59 69 59.6 + . Name=1;ID=1-409-phiX174;pvalue=1.1e-06;sequence=AAAAATTATCT; +phiX174 fimo polypeptide_motif 61 71 61.8 + . Name=1;ID=1-329-phiX174;pvalue=6.52e-07;sequence=AAATTATCTTG; +phiX174 fimo polypeptide_motif 63 73 59.2 + . Name=1;ID=1-419-phiX174;pvalue=1.2e-06;sequence=ATTATCTTGAT; +phiX174 fimo polypeptide_motif 65 75 53.3 + . Name=1;ID=1-643-phiX174;pvalue=4.66e-06;sequence=TATCTTGATAA; +phiX174 fimo polypeptide_motif 66 76 51.8 + . Name=1;ID=1-737-phiX174;pvalue=6.54e-06;sequence=ATCTTGATAAA; +phiX174 fimo polypeptide_motif 67 77 73.2 + . Name=1;ID=1-89-phiX174;pvalue=4.78e-08;sequence=TCTTGATAAAG; +phiX174 fimo polypeptide_motif 69 79 63.8 + . Name=1;ID=1-268-phiX174;pvalue=4.15e-07;sequence=TTGATAAAGCA; +phiX174 fimo polypeptide_motif 71 81 40.2 + . Name=1;ID=1-1882-phiX174;pvalue=9.49e-05;sequence=GATAAAGCAGG; +phiX174 fimo polypeptide_motif 73 83 45.4 + . Name=1;ID=1-1334-phiX174;pvalue=2.87e-05;sequence=TAAAGCAGGAA; +phiX174 fimo polypeptide_motif 74 84 50.9 + . Name=1;ID=1-832-phiX174;pvalue=8.05e-06;sequence=AAAGCAGGAAT; +phiX174 fimo polypeptide_motif 76 86 52.2 + . Name=1;ID=1-710-phiX174;pvalue=5.96e-06;sequence=AGCAGGAATTA; +phiX174 fimo polypeptide_motif 78 88 51.8 + . Name=1;ID=1-741-phiX174;pvalue=6.65e-06;sequence=CAGGAATTACT; +phiX174 fimo polypeptide_motif 79 89 45 + . Name=1;ID=1-1369-phiX174;pvalue=3.16e-05;sequence=AGGAATTACTA; +phiX174 fimo polypeptide_motif 80 90 43.3 + . Name=1;ID=1-1511-phiX174;pvalue=4.63e-05;sequence=GGAATTACTAC; +phiX174 fimo polypeptide_motif 81 91 59.8 + . Name=1;ID=1-402-phiX174;pvalue=1.05e-06;sequence=GAATTACTACT; +phiX174 fimo polypeptide_motif 82 92 46.9 + . Name=1;ID=1-1205-phiX174;pvalue=2.03e-05;sequence=AATTACTACTG; +phiX174 fimo polypeptide_motif 88 98 41.2 + . Name=1;ID=1-1744-phiX174;pvalue=7.51e-05;sequence=TACTGCTTGTT; +phiX174 fimo polypeptide_motif 91 101 53.6 + . Name=1;ID=1-621-phiX174;pvalue=4.33e-06;sequence=TGCTTGTTTAC; +phiX174 fimo polypeptide_motif 92 102 44.8 + . Name=1;ID=1-1388-phiX174;pvalue=3.31e-05;sequence=GCTTGTTTACG; +phiX174 fimo polypeptide_motif 93 103 43.2 + . Name=1;ID=1-1525-phiX174;pvalue=4.82e-05;sequence=CTTGTTTACGA; +phiX174 fimo polypeptide_motif 95 105 61.9 + . Name=1;ID=1-327-phiX174;pvalue=6.5e-07;sequence=TGTTTACGAAT; +phiX174 fimo polypeptide_motif 96 106 42.9 + . Name=1;ID=1-1553-phiX174;pvalue=5.08e-05;sequence=GTTTACGAATT; +phiX174 fimo polypeptide_motif 98 108 45.4 + . Name=1;ID=1-1340-phiX174;pvalue=2.9e-05;sequence=TTACGAATTAA; +phiX174 fimo polypeptide_motif 99 109 73.5 + . Name=1;ID=1-88-phiX174;pvalue=4.48e-08;sequence=TACGAATTAAA; +phiX174 fimo polypeptide_motif 100 110 53 + . Name=1;ID=1-661-phiX174;pvalue=4.99e-06;sequence=ACGAATTAAAT; +phiX174 fimo polypeptide_motif 102 112 75.6 + . Name=1;ID=1-72-phiX174;pvalue=2.75e-08;sequence=GAATTAAATCG; +phiX174 fimo polypeptide_motif 104 114 52.2 + . Name=1;ID=1-713-phiX174;pvalue=6.05e-06;sequence=ATTAAATCGAA; +phiX174 fimo polypeptide_motif 106 116 59.6 + . Name=1;ID=1-407-phiX174;pvalue=1.09e-06;sequence=TAAATCGAAGT; +phiX174 fimo polypeptide_motif 112 122 41.6 + . Name=1;ID=1-1683-phiX174;pvalue=6.85e-05;sequence=GAAGTGGACTG; +phiX174 fimo polypeptide_motif 114 124 45.3 + . Name=1;ID=1-1342-phiX174;pvalue=2.92e-05;sequence=AGTGGACTGCT; +phiX174 fimo polypeptide_motif 118 128 56.2 + . Name=1;ID=1-512-phiX174;pvalue=2.38e-06;sequence=GACTGCTGGCG; +phiX174 fimo polypeptide_motif 122 132 51.4 + . Name=1;ID=1-774-phiX174;pvalue=7.15e-06;sequence=GCTGGCGGAAA; +phiX174 fimo polypeptide_motif 123 133 43.1 + . Name=1;ID=1-1533-phiX174;pvalue=4.93e-05;sequence=CTGGCGGAAAA; +phiX174 fimo polypeptide_motif 124 134 48.6 + . Name=1;ID=1-1085-phiX174;pvalue=1.38e-05;sequence=TGGCGGAAAAT; +phiX174 fimo polypeptide_motif 125 135 68.3 + . Name=1;ID=1-146-phiX174;pvalue=1.49e-07;sequence=GGCGGAAAATG; +phiX174 fimo polypeptide_motif 126 136 46.4 + . Name=1;ID=1-1243-phiX174;pvalue=2.27e-05;sequence=GCGGAAAATGA; +phiX174 fimo polypeptide_motif 128 138 58.3 + . Name=1;ID=1-446-phiX174;pvalue=1.48e-06;sequence=GGAAAATGAGA; +phiX174 fimo polypeptide_motif 129 139 43.2 + . Name=1;ID=1-1522-phiX174;pvalue=4.78e-05;sequence=GAAAATGAGAA; +phiX174 fimo polypeptide_motif 130 140 54.1 + . Name=1;ID=1-595-phiX174;pvalue=3.92e-06;sequence=AAAATGAGAAA; +phiX174 fimo polypeptide_motif 131 141 76 + . Name=1;ID=1-68-phiX174;pvalue=2.49e-08;sequence=AAATGAGAAAA; +phiX174 fimo polypeptide_motif 132 142 51.2 + . Name=1;ID=1-800-phiX174;pvalue=7.57e-06;sequence=AATGAGAAAAT; +phiX174 fimo polypeptide_motif 133 143 56.2 + . Name=1;ID=1-513-phiX174;pvalue=2.41e-06;sequence=ATGAGAAAATT; +phiX174 fimo polypeptide_motif 134 144 41.1 + . Name=1;ID=1-1761-phiX174;pvalue=7.83e-05;sequence=TGAGAAAATTC; +phiX174 fimo polypeptide_motif 135 145 50.3 + . Name=1;ID=1-910-phiX174;pvalue=9.39e-06;sequence=GAGAAAATTCG; +phiX174 fimo polypeptide_motif 136 146 43.3 + . Name=1;ID=1-1517-phiX174;pvalue=4.66e-05;sequence=AGAAAATTCGA; +phiX174 fimo polypeptide_motif 139 149 54.2 + . Name=1;ID=1-588-phiX174;pvalue=3.75e-06;sequence=AAATTCGACCT; +phiX174 fimo polypeptide_motif 141 151 42.2 + . Name=1;ID=1-1625-phiX174;pvalue=6.01e-05;sequence=ATTCGACCTAT; +phiX174 fimo polypeptide_motif 143 153 50 + . Name=1;ID=1-938-phiX174;pvalue=9.94e-06;sequence=TCGACCTATCC; +phiX174 fimo polypeptide_motif 145 155 44.6 + . Name=1;ID=1-1403-phiX174;pvalue=3.42e-05;sequence=GACCTATCCTT; +phiX174 fimo polypeptide_motif 155 165 51.3 + . Name=1;ID=1-787-phiX174;pvalue=7.35e-06;sequence=TGCGCAGCTCG; +phiX174 fimo polypeptide_motif 157 167 51.1 + . Name=1;ID=1-807-phiX174;pvalue=7.68e-06;sequence=CGCAGCTCGAG; +phiX174 fimo polypeptide_motif 159 169 44.5 + . Name=1;ID=1-1420-phiX174;pvalue=3.56e-05;sequence=CAGCTCGAGAA; +phiX174 fimo polypeptide_motif 160 170 40 + . Name=1;ID=1-1921-phiX174;pvalue=9.89e-05;sequence=AGCTCGAGAAG; +phiX174 fimo polypeptide_motif 166 176 60.9 + . Name=1;ID=1-365-phiX174;pvalue=8.02e-07;sequence=AGAAGCTCTTA; +phiX174 fimo polypeptide_motif 168 178 62.3 + . Name=1;ID=1-311-phiX174;pvalue=5.87e-07;sequence=AAGCTCTTACT; +phiX174 fimo polypeptide_motif 181 191 49.9 + . Name=1;ID=1-946-phiX174;pvalue=1.01e-05;sequence=GCGACCTTTCG; +phiX174 fimo polypeptide_motif 187 197 52.5 + . Name=1;ID=1-694-phiX174;pvalue=5.64e-06;sequence=TTTCGCCATCA; +phiX174 fimo polypeptide_motif 191 201 46.6 + . Name=1;ID=1-1232-phiX174;pvalue=2.2e-05;sequence=GCCATCAACTA; +phiX174 fimo polypeptide_motif 194 204 76.4 + . Name=1;ID=1-67-phiX174;pvalue=2.29e-08;sequence=ATCAACTAACG; +phiX174 fimo polypeptide_motif 201 211 40.1 + . Name=1;ID=1-1908-phiX174;pvalue=9.77e-05;sequence=AACGATTCTGT; +phiX174 fimo polypeptide_motif 203 213 63 + . Name=1;ID=1-291-phiX174;pvalue=5e-07;sequence=CGATTCTGTCA; +phiX174 fimo polypeptide_motif 205 215 53.8 + . Name=1;ID=1-610-phiX174;pvalue=4.16e-06;sequence=ATTCTGTCAAA; +phiX174 fimo polypeptide_motif 206 216 59.1 + . Name=1;ID=1-421-phiX174;pvalue=1.23e-06;sequence=TTCTGTCAAAA; +phiX174 fimo polypeptide_motif 207 217 68 + . Name=1;ID=1-153-phiX174;pvalue=1.58e-07;sequence=TCTGTCAAAAA; +phiX174 fimo polypeptide_motif 209 219 49.6 + . Name=1;ID=1-988-phiX174;pvalue=1.09e-05;sequence=TGTCAAAAACT; +phiX174 fimo polypeptide_motif 210 220 40.8 + . Name=1;ID=1-1810-phiX174;pvalue=8.33e-05;sequence=GTCAAAAACTG; +phiX174 fimo polypeptide_motif 213 223 59.7 + . Name=1;ID=1-404-phiX174;pvalue=1.06e-06;sequence=AAAAACTGACG; +phiX174 fimo polypeptide_motif 223 233 42 + . Name=1;ID=1-1654-phiX174;pvalue=6.36e-05;sequence=GCGTTGGATGA; +phiX174 fimo polypeptide_motif 225 235 61.4 + . Name=1;ID=1-349-phiX174;pvalue=7.16e-07;sequence=GTTGGATGAGG; +phiX174 fimo polypeptide_motif 227 237 40.3 + . Name=1;ID=1-1874-phiX174;pvalue=9.32e-05;sequence=TGGATGAGGAG; +phiX174 fimo polypeptide_motif 228 238 49.9 + . Name=1;ID=1-947-phiX174;pvalue=1.01e-05;sequence=GGATGAGGAGA; +phiX174 fimo polypeptide_motif 229 239 45 + . Name=1;ID=1-1370-phiX174;pvalue=3.16e-05;sequence=GATGAGGAGAA; +phiX174 fimo polypeptide_motif 230 240 44.8 + . Name=1;ID=1-1395-phiX174;pvalue=3.33e-05;sequence=ATGAGGAGAAG;
--- a/test-data/prior30.plib Tue Mar 08 08:10:52 2016 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,275 +0,0 @@ -Alphabet= ACDEFGHIKLMNPQRSTVWY -NumDistr= 30 -Number= 0 -Mixture= 0.055795 -B= 5.623820 -Alpha= 0.0855491 0.0221831 0.0111063 0.0209959 0.0505726 0.025437 0.0155389 0.132951 0.0247865 0.150287 0.0577239 0.0209317 0.0166629 0.0220905 0.0244295 0.0497608 0.070277 0.157532 0.0102219 0.0309633 -FullUpdate= 1 -QUpdate= 1 -StructID= 0 -Comment= HMM9.4 reestimated in henikoff29.2 - -Number= 1 -Mixture= 0.198333 -B= 0.097240 -Alpha= 0.0562629 0.0329597 0.0692513 0.0385232 0.0400041 0.143573 0.0428939 0.0226244 0.0442102 0.0665467 0.0117853 0.0447655 0.0833299 0.0395825 0.0611271 0.0588852 0.0513472 0.0317153 0.0237865 0.0368161 -FullUpdate= 1 -QUpdate= 1 -StructID= 24 -Comment= Outside - -Number= 2 -Mixture= 0.043566 -B= 1.648336 -Alpha= 0.0144564 0.00845337 0.00785519 0.00864933 0.255959 0.0110815 0.0509526 0.0234533 0.0120443 0.0561967 0.015111 0.0190974 0.00857653 0.0167812 0.0164918 0.0197108 0.0151013 0.0252782 0.050139 0.364613 -FullUpdate= 1 -QUpdate= 1 -StructID= 26 -Comment= Inside - -Number= 3 -Mixture= 0.060170 -B= 2.595432 -Alpha= 0.0452144 0.00587917 0.169731 0.0751478 0.00749471 0.0845832 0.0369819 0.00610072 0.0548186 0.011029 0.00382749 0.212785 0.0206532 0.0416705 0.0280716 0.117267 0.0533742 0.00943157 0.00216149 0.0137784 -FullUpdate= 1 -QUpdate= 1 -StructID= 19 -Comment= Outside Alpha - -Number= 4 -Mixture= 0.065466 -B= 3.112271 -Alpha= 0.0361167 0.0049157 0.0134924 0.0461325 0.00557631 0.0209043 0.0302551 0.016425 0.307554 0.0338255 0.0139435 0.0360733 0.0127659 0.0873761 0.222668 0.0369042 0.0354442 0.0228891 0.00434827 0.0123906 -FullUpdate= 1 -QUpdate= 1 -StructID= 21 -Comment= Outside Beta - -Number= 5 -Mixture= 0.067614 -B= 2.053644 -Alpha= 0.0194362 0.00765176 0.00188738 0.00372898 0.0849894 0.00421787 0.00400459 0.152735 0.00407958 0.4568 0.106051 0.00304386 0.00545956 0.00900935 0.00605071 0.00519029 0.016255 0.0861045 0.00787965 0.0154248 -FullUpdate= 1 -QUpdate= 1 -StructID= 22 -Comment= Inside alpha - -Number= 6 -Mixture= 0.080724 -B= 2.138987 -Alpha= 0.0423172 0.0153891 0.00409306 0.00565735 0.0197117 0.00590607 0.00139926 0.307863 0.00544884 0.115721 0.0285808 0.00522771 0.00474851 0.00328193 0.00351054 0.00892385 0.0348922 0.380003 0.00117673 0.00614917 -FullUpdate= 1 -QUpdate= 1 -StructID= 23 -Comment= Inside beta - 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--- a/tool_dependencies.xml Tue Mar 08 08:10:52 2016 -0500 +++ b/tool_dependencies.xml Fri Jun 17 13:15:48 2016 -0400 @@ -4,6 +4,6 @@ <repository changeset_revision="942ae5bafee3" name="package_imagemagick_6_9_3" owner="iuc" prior_installation_required="True" toolshed="https://toolshed.g2.bx.psu.edu" /> </package> <package name="meme" version="4.11.0"> - <repository changeset_revision="738dc831aeac" name="package_meme_4_11_0" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" /> + <repository changeset_revision="5774a0e96d6f" name="package_meme_4_11_0" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" /> </package> </tool_dependency>