# HG changeset patch # User iuc # Date 1524672788 14400 # Node ID 793225b112029011888d89f02af25b815d49e69f # Parent a0fa4efeeee33ada1bccd6382820362027a0e8eb planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meme commit 42fa6e319cf1a97330818dc8c869871a32f0e7aa diff -r a0fa4efeeee3 -r 793225b11202 test-data/dreme1.html --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/dreme1.html Wed Apr 25 12:13:08 2018 -0400 @@ -0,0 +1,6199 @@ + + + + + DREME + + + + + + + + + + + + + + +
+

Help poup.

+
[close ]
+
+ +
+

+ The name of the motif uses the IUPAC codes for nucleotides which has + a different letter to represent each of the 15 possible combinations. +

+

+ The name is itself a representation of the motif though the position + weight matrix is not directly equalivant as it is generated from the + sites found that matched the letters given in the name. +

+

+ + Read more about the MEME suite's use of the IUPAC alphabets. + + +

+
[close ]
+
+ + +
+

The E-value is the enrichment p-value times the number of candidate + motifs tested.

+

The enrichment p-value is calculated using Fisher's Exact Test for + enrichment of the motif in the positive sequences.

+

Note that the counts used in Fisher's Exact Test are made after + erasing sites that match previously found motifs.

+
[close ]
+
+
+

The E-value of the motif calculated without erasing the sites of + previously found motifs.

+
[close ]
+
+
+

Show more information on the motif.

+
[close ]
+
+
+

Submit your motif to another MEME Suite program or download your motif.

+
Supported Programs
+
+
Tomtom
+
Tomtom is a tool for searching for similar known motifs. +
+
MAST
+
MAST is a tool for searching biological sequence databases for + sequences that contain one or more of a group of known motifs. +
+
FIMO
+
FIMO is a tool for searching biological sequence databases for + sequences that contain one or more known motifs. +
+
GOMO
+
GOMO is a tool for identifying possible roles (Gene Ontology + terms) for DNA binding motifs. +
+
SpaMo
+
SpaMo is a tool for inferring possible transcription factor + complexes by finding motifs with enriched spacings. +
+
+
[close ]
+
+
+

# positive sequences matching the motif / # positive sequences.

+

Note these counts are made after erasing sites that match previously + found motifs.

+
[close ]
+
+
+

# negative sequences matching the motif / # negative sequences.

+

Note these counts are made after erasing sites that match previously + found motifs.

+
[close ]
+
+
+

The p-value of Fisher's Exact Test for enrichment of the motif in + the positive sequences.

+

Note that the counts used in Fisher's Exact Test are made after + erasing sites that match previously found motifs.

+
[close ]
+
+
+

The E-value is the motif p-value times the number of candidate motifs + tested.

+

Note that the p-value was calculated with counts made after + erasing sites that match previously found motifs.

+
[close ]
+
+
+

The E-value of the motif calculated without erasing the sites of + previously found motifs.

+
[close ]
+
+
+

All words matching the motif whose uncorrected p-value is less than + .

+ +
[close ]
+
+
+

# positive sequences with matches to the word / # positive sequences.

+

Note these counts are made after erasing sites that match previously + found motifs.

+
[close ]
+
+
+

# negative sequences with matches to the word / # negative sequences.

+

Note these counts are made after erasing sites that match previously + found motifs.

+
[close ]
+
+
+

The p-value of Fisher's Exact Test for enrichment of the word in + the positive sequences.

+

Note that the counts used in Fisher's Exact Test are made after + erasing sites that match previously found motifs.

+
[close ]
+
+
+

The word p-value times the number of candidates tested.

+

Note that the p-value was calculated with counts made after + erasing sites that match previously found motifs.

+
[close ]
+
+ +
+

The sequence file used by DREME to find the motifs.

+
[close ]
+
+
+

The alphabet of the sequences.

+
[close ]
+
+
+

The count of the sequences.

+
[close ]
+
+ +
+

The name of the alphabet symbol.

+
[close ]
+
+ +
+

The frequency of the alphabet symbol in the control dataset.

+
[close ]
+
+ + + +
+
+ + +
+

Details

+ + + + + + + + + + + + + + + + + + + +
Positives
Negatives
P-value
E-value
Unerased E-value
+ / + + / +
+

Enriched Matching Words

+
+
+ + + + + + + +
+ +

+ For further information on how to interpret these results or to get a + copy of the MEME software please access + http://meme.nbcr.net. +

+

+ If you use DREME in your research please cite the following paper:
+ + Timothy L. Bailey, "DREME: Motif discovery in transcription factor ChIP-seq data", Bioinformatics, 27(12):1653-1659, 2011. + [full text] + +

+
+ +
+ Discovered Motifs +   |   + Inputs & Settings +   |   + Program information +
+ + +

Your browser does not support canvas!

+ + + + + +
+

Discovered Motifs

+ Next Top +
+
+

No motifs were discovered!

+
+ + +
+

Inputs & Settings

+ Previous Next Top +
+
+

Sequences

+ + + + + + + + + + +
Source
Alphabet
Sequence Count
+ +

Control Sequences

+ + + + + + + + +
Source
Sequence Count
+ +

Background

+ + +

Other Settings

+ + + + + + + + + + +
Strand Handling + This alphabet only has one strand + Only the given strand is processed + Both the given and reverse complement strands are processed +
# REs to Generalize
Shuffle Seed
E-value Threshold
Max Motif Count
Max Run Time
+ +
+ +
+
Previous Top
+
+
DREME version
+ + (Release date: )
+
+ +
+
Reference
+ + Timothy L. Bailey, "DREME: Motif discovery in transcription factor ChIP-seq data", Bioinformatics, 27(12):1653-1659, 2011. + [full text] + +
+
+
Command line
+ + +
+
+ + + diff -r a0fa4efeeee3 -r 793225b11202 test-data/dreme1.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/dreme1.txt Wed Apr 25 12:13:08 2018 -0400 @@ -0,0 +1,102 @@ +# DREME 4.12.0 +# command: dreme -oc dreme_out -rna -norc -p dreme_test_sites.fa -e 0.05 -mink 3 -maxk 8 +# positives: 1000 from dreme_test_sites.fa (Thu Apr 19 19:09:45 CEST 2018) +# negatives: 1000 from shuffled positives +# host: ThinkPad-T450s +# when: Thu Apr 19 19:11:17 CEST 2018 + +MEME version 4.12.0 + +ALPHABET "RNA" RNA-LIKE +A "Adenine" CC0000 +C "Cytosine" 0000CC +G "Guanine" FFB300 +U "Uracil" 008000 +N "Any base" = ACGU +X = ACGU +. = ACGU +V "Not U" = ACG +H "Not G" = ACU +D "Not C" = AGU +B "Not A" = CGU +M "Amino" = AC +R "Purine" = AG +W "Weak" = AU +S "Strong" = CG +Y "Pyrimidine" = CU +K "Keto" = GU +T = U +END ALPHABET + +Background letter frequencies (from dataset): +A 0.221 C 0.245 G 0.221 U 0.312 + + +MOTIF UUYUCY DREME-1 + +# Word Pos Neg P-value E-value +# BEST UUYUCY 387 210 2.6e-018 1.2e-013 +# UUUUCC 147 75 1.8e-007 8.1e-003 +# UUUUCU 155 94 2.2e-005 1.0e+000 +# UUCUCU 94 51 1.3e-004 6.1e+000 +# UUCUCC 75 42 1.1e-003 5.0e+001 + +letter-probability matrix: alength= 4 w= 6 nsites= 459 E= 1.2e-013 +0.000000 0.000000 0.000000 1.000000 +0.000000 0.000000 0.000000 1.000000 +0.000000 0.294118 0.000000 0.705882 +0.000000 0.000000 0.000000 1.000000 +0.000000 1.000000 0.000000 0.000000 +0.000000 0.474946 0.000000 0.525054 + + +MOTIF YAGG DREME-2 + +# Word Pos Neg P-value E-value +# BEST YAGG 600 416 1.1e-016 5.1e-012 +# CAGG 441 304 1.5e-010 6.6e-006 +# UAGG 232 165 1.1e-004 4.7e+000 + +letter-probability matrix: alength= 4 w= 4 nsites= 793 E= 5.1e-012 +0.000000 0.692308 0.000000 0.307692 +1.000000 0.000000 0.000000 0.000000 +0.000000 0.000000 1.000000 0.000000 +0.000000 0.000000 1.000000 0.000000 + + +MOTIF GAAGAW DREME-3 + +# Word Pos Neg P-value E-value +# BEST GAAGAW 81 22 8.2e-010 3.4e-005 +# GAAGAU 45 7 2.4e-008 9.9e-004 +# GAAGAA 40 16 7.9e-004 3.3e+001 + +letter-probability matrix: alength= 4 w= 6 nsites= 89 E= 3.4e-005 +0.000000 0.000000 1.000000 0.000000 +1.000000 0.000000 0.000000 0.000000 +1.000000 0.000000 0.000000 0.000000 +0.000000 0.000000 1.000000 0.000000 +1.000000 0.000000 0.000000 0.000000 +0.494382 0.000000 0.000000 0.505618 + + +MOTIF SMUGGA DREME-4 + +# Word Pos Neg P-value E-value +# BEST SMUGGA 110 47 9.1e-008 3.7e-003 +# GAUGGA 22 6 1.7e-003 7.1e+001 +# GCUGGA 33 14 3.6e-003 1.5e+002 +# CCUGGA 32 15 8.6e-003 3.5e+002 +# CAUGGA 29 13 9.1e-003 3.7e+002 + +letter-probability matrix: alength= 4 w= 6 nsites= 119 E= 3.7e-003 +0.000000 0.529412 0.470588 0.000000 +0.428571 0.571429 0.000000 0.000000 +0.000000 0.000000 0.000000 1.000000 +0.000000 0.000000 1.000000 0.000000 +0.000000 0.000000 1.000000 0.000000 +1.000000 0.000000 0.000000 0.000000 + + +# Stopping reason: E-value threshold exceeded +# Running time: 13.45 seconds diff -r a0fa4efeeee3 -r 793225b11202 test-data/dreme1.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/dreme1.xml Wed Apr 25 12:13:08 2018 -0400 @@ -0,0 +1,32 @@ + + + dreme -oc dreme_out -rna -norc -p dreme_test_sites.fa -e 0 + + + + + + + + + + + + + + + + + + + + none + + + 100 + 0.01 + 1 + ThinkPad-T450s + Thu Apr 19 19:40:08 CEST 2018 + + diff -r a0fa4efeeee3 -r 793225b11202 test-data/dreme2.html --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/dreme2.html Wed Apr 25 12:13:08 2018 -0400 @@ -0,0 +1,6118 @@ + + + + + DREME + + + + + + + + + + + + + + +
+

Help poup.

+ +
+ +
+

+ The name of the motif uses the IUPAC codes for nucleotides which has + a different letter to represent each of the 15 possible combinations. +

+

+ The name is itself a representation of the motif though the position + weight matrix is not directly equalivant as it is generated from the + sites found that matched the letters given in the name. +

+

+ + Read more about the MEME suite's use of the IUPAC alphabets. + + +

+ +
+ + +
+

The E-value is the enrichment p-value times the number of candidate + motifs tested.

+

The enrichment p-value is calculated using Fisher's Exact Test for + enrichment of the motif in the positive sequences.

+

Note that the counts used in Fisher's Exact Test are made after + erasing sites that match previously found motifs.

+ +
+
+

The E-value of the motif calculated without erasing the sites of + previously found motifs.

+ +
+
+

Show more information on the motif.

+ +
+
+

Submit your motif to another MEME Suite program or download your motif.

+
Supported Programs
+
+
Tomtom
+
Tomtom is a tool for searching for similar known motifs. +
+
MAST
+
MAST is a tool for searching biological sequence databases for + sequences that contain one or more of a group of known motifs. +
+
FIMO
+
FIMO is a tool for searching biological sequence databases for + sequences that contain one or more known motifs. +
+
GOMO
+
GOMO is a tool for identifying possible roles (Gene Ontology + terms) for DNA binding motifs. +
+
SpaMo
+
SpaMo is a tool for inferring possible transcription factor + complexes by finding motifs with enriched spacings. +
+
+ +
+
+

# positive sequences matching the motif / # positive sequences.

+

Note these counts are made after erasing sites that match previously + found motifs.

+ +
+
+

# negative sequences matching the motif / # negative sequences.

+

Note these counts are made after erasing sites that match previously + found motifs.

+ +
+
+

The p-value of Fisher's Exact Test for enrichment of the motif in + the positive sequences.

+

Note that the counts used in Fisher's Exact Test are made after + erasing sites that match previously found motifs.

+ +
+
+

The E-value is the motif p-value times the number of candidate motifs + tested.

+

Note that the p-value was calculated with counts made after + erasing sites that match previously found motifs.

+ +
+
+

The E-value of the motif calculated without erasing the sites of + previously found motifs.

+ +
+
+

All words matching the motif whose uncorrected p-value is less than + .

+ + +
+
+

# positive sequences with matches to the word / # positive sequences.

+

Note these counts are made after erasing sites that match previously + found motifs.

+ +
+
+

# negative sequences with matches to the word / # negative sequences.

+

Note these counts are made after erasing sites that match previously + found motifs.

+ +
+
+

The p-value of Fisher's Exact Test for enrichment of the word in + the positive sequences.

+

Note that the counts used in Fisher's Exact Test are made after + erasing sites that match previously found motifs.

+ +
+
+

The word p-value times the number of candidates tested.

+

Note that the p-value was calculated with counts made after + erasing sites that match previously found motifs.

+ +
+ +
+

The sequence file used by DREME to find the motifs.

+ +
+
+

The alphabet of the sequences.

+ +
+
+

The count of the sequences.

+ +
+ +
+

The name of the alphabet symbol.

+ +
+ +
+

The frequency of the alphabet symbol in the control dataset.

+ +
+ + + +
+
+ + +
+

Details

+ + + + + + + + + + + + + + + + + + + +
Positives
Negatives
P-value
E-value
Unerased E-value
+ / + + / +
+

Enriched Matching Words

+
+
+ + + + + + + +
+ +

+ For further information on how to interpret these results or to get a + copy of the MEME software please access + http://meme.nbcr.net. +

+

+ If you use DREME in your research please cite the following paper:
+ + Timothy L. Bailey, "DREME: Motif discovery in transcription factor ChIP-seq data", Bioinformatics, 27(12):1653-1659, 2011. + [full text] + +

+
+ +
+ Discovered Motifs +   |   + Inputs & Settings +   |   + Program information +
+ + +

Your browser does not support canvas!

+ + + + + +
+

Discovered Motifs

+ Next Top +
+
+

No motifs were discovered!

+
+ + +
+

Inputs & Settings

+ Previous Next Top +
+
+

Sequences

+ + + + + + + + + + +
Source
Alphabet
Sequence Count
+ +

Control Sequences

+ + + + + + + + +
Source
Sequence Count
+ +

Background

+ + +

Other Settings

+ + + + + + + + + + +
Strand Handling + This alphabet only has one strand + Only the given strand is processed + Both the given and reverse complement strands are processed +
# REs to Generalize
Shuffle Seed
E-value Threshold
Max Motif Count
Max Run Time
+ +
+ +
+
Previous Top
+
+
DREME version
+ + (Release date: )
+
+ +
+
Reference
+ + Timothy L. Bailey, "DREME: Motif discovery in transcription factor ChIP-seq data", Bioinformatics, 27(12):1653-1659, 2011. + [full text] + +
+
+
Command line
+ + +
+
+ + + diff -r a0fa4efeeee3 -r 793225b11202 test-data/dreme2.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/dreme2.txt Wed Apr 25 12:13:08 2018 -0400 @@ -0,0 +1,68 @@ +# DREME 4.12.0 +# command: dreme -oc dreme_out_adv -rna -norc -p dreme_test_sites.fa -e 0.00001 -mink 4 -maxk 10 +# positives: 1000 from dreme_test_sites.fa (Thu Apr 19 19:09:45 CEST 2018) +# negatives: 1000 from shuffled positives +# host: ThinkPad-T450s +# when: Tue Apr 24 18:44:36 CEST 2018 + +MEME version 4.12.0 + +ALPHABET "RNA" RNA-LIKE +A "Adenine" CC0000 +C "Cytosine" 0000CC +G "Guanine" FFB300 +U "Uracil" 008000 +N "Any base" = ACGU +X = ACGU +. = ACGU +V "Not U" = ACG +H "Not G" = ACU +D "Not C" = AGU +B "Not A" = CGU +M "Amino" = AC +R "Purine" = AG +W "Weak" = AU +S "Strong" = CG +Y "Pyrimidine" = CU +K "Keto" = GU +T = U +END ALPHABET + +Background letter frequencies (from dataset): +A 0.221 C 0.245 G 0.221 U 0.312 + + +MOTIF UUYUCY DREME-1 + +# Word Pos Neg P-value E-value +# BEST UUYUCY 387 210 2.6e-018 3.3e-013 +# UUUUCC 147 75 1.8e-007 2.2e-002 +# UUUUCU 155 94 2.2e-005 2.8e+000 +# UUCUCU 94 51 1.3e-004 1.7e+001 +# UUCUCC 75 42 1.1e-003 1.4e+002 + +letter-probability matrix: alength= 4 w= 6 nsites= 459 E= 3.3e-013 +0.000000 0.000000 0.000000 1.000000 +0.000000 0.000000 0.000000 1.000000 +0.000000 0.294118 0.000000 0.705882 +0.000000 0.000000 0.000000 1.000000 +0.000000 1.000000 0.000000 0.000000 +0.000000 0.474946 0.000000 0.525054 + + +MOTIF YAGG DREME-2 + +# Word Pos Neg P-value E-value +# BEST YAGG 600 416 1.1e-016 1.4e-011 +# CAGG 441 304 1.5e-010 1.8e-005 +# UAGG 232 165 1.1e-004 1.3e+001 + +letter-probability matrix: alength= 4 w= 4 nsites= 793 E= 1.4e-011 +0.000000 0.692308 0.000000 0.307692 +1.000000 0.000000 0.000000 0.000000 +0.000000 0.000000 1.000000 0.000000 +0.000000 0.000000 1.000000 0.000000 + + +# Stopping reason: E-value threshold exceeded +# Running time: 18.17 seconds diff -r a0fa4efeeee3 -r 793225b11202 test-data/dreme_test_sites.fa --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/dreme_test_sites.fa Wed Apr 25 12:13:08 2018 -0400 @@ -0,0 +1,2000 @@ +>U2AF2_K562_rep02_1 +UCUACACAUAGGGUGUUCCUGAAAAGCCCCAUAGUGAUUGAGUCUUCAAAACCACCGAUUC +>U2AF2_K562_rep02_2 +UUGGAUAGCGGAAGCCCUGACCAGGCAUGGAGUAUCUCACAAAGUAGACGAUUCCUCUGGG +>U2AF2_K562_rep02_3 +GGUUUCGGUCUAAGUACCACCCAGAUGAGGUGGGGAAGCGUCGGCAGGAGGCCCGGGGGGC +>U2AF2_K562_rep02_4 +AAACAGGGUUUGUGGAUGCACUUAGAUGUUUGCAAUGAGCACUGUGGCUGGCAUGCCCCAG +>U2AF2_K562_rep02_5 +UCCCAUUGUUUCUGCAGAGGCAGUUGGUGUCACCAGCCAGCGACCAGUGUUUUGUCCUUUU +>U2AF2_K562_rep02_6 +UUCUACCCUCUCUAACUCCUCCCAGCAGCAAAAGGGAGACACUUCAGCUGGAACAAAAUAG +>U2AF2_K562_rep02_7 +UUCUACCCUCUCUAACUCCUCCCAGCAGCAAAAGGGAGACACUUCAGCUGGAACAAAAUAG +>U2AF2_K562_rep02_8 +UUUUCUCCCUUCCACCCACACCCUGCAGCGUCGAGGUCCUCAGCGAUCAGUUGAUGUAAUA +>U2AF2_K562_rep02_9 +GGCAGGGGAAGUGGGGAUGGCAGGACAUGGAUGGCCCUUGAGGCAUUGGCUCUGGGUGUCA +>U2AF2_K562_rep02_10 +GCAGGGGAAGUGGGGAUGGCAGGACAUGGAUGGCCCUUGAGGCAUUGGCUCUGGGUGUCAU +>U2AF2_K562_rep02_11 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