# HG changeset patch # User iuc # Date 1670064135 0 # Node ID 27250f92a01a98e3947e76485ef73c884af5fb5d # Parent ab1a9a932182063e6dc04861d346985d88436de3 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/metaphlan/ commit f3a89af137b13715f9fb13383577aceb2c445ce6 diff -r ab1a9a932182 -r 27250f92a01a customizemetadata.py --- a/customizemetadata.py Wed Feb 02 22:01:39 2022 +0000 +++ b/customizemetadata.py Sat Dec 03 10:42:15 2022 +0000 @@ -56,6 +56,7 @@ 'taxonomy': in_metadata['taxonomy'], 'merged_taxon': {} } + # transform merged_taxons tuple keys to string for k in in_metadata['merged_taxon']: n = ' , '.join(k) @@ -85,6 +86,13 @@ n = ' , '.split(k) out_metadata[n] = in_metadata['merged_taxon'][k] + # Ensure that there are 8 taxonomy levels (for compatibility between Metaphlan v3 and v4) + # v3 DB release encodes the taxids as: ('2|1224|1236|91347|543|547|354276', 4404432) + # v4 DB release encodes the taxids as: ('2|1224|1236|91347|543|547|354276|', 4404432) + for k in out_metadata['taxonomy']: + if out_metadata['taxonomy'][k][0].count('|') == 6: + out_metadata['taxonomy'][k] = (out_metadata['taxonomy'][k][0] + '|', out_metadata['taxonomy'][k][1]) + # dump metadata to Pickle file with bz2.BZ2File(pkl_fp, 'w') as pkl_f: pickle.dump(out_metadata, pkl_f) diff -r ab1a9a932182 -r 27250f92a01a formatoutput.py --- a/formatoutput.py Wed Feb 02 22:01:39 2022 +0000 +++ b/formatoutput.py Sat Dec 03 10:42:15 2022 +0000 @@ -57,9 +57,11 @@ # skip headers if line.startswith("#"): continue - # skip UNKNOWN lines in Predicted taxon relative abundances - if "UNKNOWN" in line: + + # skip UNKNOWN (v3) or UNCLASSIFIED (v4) lines in predicted taxon relative abundances + if "UNKNOWN" in line or 'UNCLASSIFIED' in line: continue + # spit lines split_line = line[:-1].split('\t') taxo_n = split_line[0].split('|') diff -r ab1a9a932182 -r 27250f92a01a macros.xml --- a/macros.xml Wed Feb 02 22:01:39 2022 +0000 +++ b/macros.xml Sat Dec 03 10:42:15 2022 +0000 @@ -1,6 +1,6 @@ - 3.0.14 + 4.0.3 0 20.01 diff -r ab1a9a932182 -r 27250f92a01a merge_metaphlan_tables.xml --- a/merge_metaphlan_tables.xml Wed Feb 02 22:01:39 2022 +0000 +++ b/merge_metaphlan_tables.xml Sat Dec 03 10:42:15 2022 +0000 @@ -27,8 +27,8 @@ - + @@ -38,7 +38,7 @@ ============ MetaPhlAn is a computational tool for profiling the composition of microbial communities (Bacteria, -Archaea and Eukaryotes) from metagenomic shotgun sequencing data (i.e. not 16S) with species-level. +Archaea and Eukaryotes) from metagenomic shotgun sequencing data (i.e. not 16S) at the species-level. This tool performs a table join on one or more tab-separated files with the predicted taxon relative abundances that have been generated by MetaPhlAn. diff -r ab1a9a932182 -r 27250f92a01a test-data/test-db.json --- a/test-data/test-db.json Wed Feb 02 22:01:39 2022 +0000 +++ b/test-data/test-db.json Sat Dec 03 10:42:15 2022 +0000 @@ -1,1 +1,1 @@ -{"markers": {"504553__GeneID:10498608": {"ext": ["GCA_000144505", "GCA_000144125", "GCA_000488875", "GCA_000178055", "GCA_001660965", "GCA_000144445", "GCA_003384145", "GCA_002572705", "GCA_001660935", "GCA_001750525", "GCA_000144775", "GCA_003390995", "GCA_001750535", "GCA_000008345", "GCA_003384705", "GCA_003384485", "GCA_002572745", "GCA_000144465", "GCA_000145375", "GCA_002775655", "GCA_001660855", "GCA_001660945", "GCA_000144875", "GCA_003426625", "GCA_000240055", "GCA_003426225", "GCA_003384285", "GCA_000144815", "GCA_000145095", "GCA_900102845", "GCA_001469595", "GCA_000145195", "GCA_003384365", "GCA_000145155", "GCA_003384255", "GCA_003384315", "GCA_000144345", "GCA_000240015", "GCA_000302515", "GCA_002831715", "GCA_000144285", "GCA_003426665", "GCA_001469635", "GCA_000144185", "GCA_002572835", "GCA_001660115", "GCA_000144325", "GCA_000144735", "GCA_000194825", "GCA_000376705", "GCA_000147145", "GCA_002572575", "GCA_000144795", "GCA_003384385", "GCA_000144895", "GCA_003425775", "GCA_003384345", "GCA_000231215", "GCA_003384555", "GCA_000735055", "GCA_000145575", "GCA_000144005", "GCA_003384195", "GCA_001469555", "GCA_003384585", "GCA_003384395", "GCA_000145455", "GCA_000144485", "GCA_003384495", "GCA_000342585", "GCA_003384445", "GCA_000144365", "GCA_000730485", "GCA_002861085", "GCA_002572615", "GCA_000144145", "GCA_002556485", "GCA_001481615", "GCA_000144245", "GCA_000144545", "GCA_000221125", "GCA_001469655", "GCA_003426685", "GCA_000144045", "GCA_002572665", "GCA_002572695", "GCA_002572655", "GCA_001750555", "GCA_003426255", "GCA_000145335", "GCA_002572775"], "score": 37.0, "clade": "t__Propionibacterium_phage_PAS50", "len": 663, "taxon": "k__Viruses|p__Viruses_unclassified|c__Viruses_unclassified|o__Caudovirales|f__Siphoviridae|g__Pa6virus|s__Propionibacterium_virus_PAS50|t__Propionibacterium_phage_PAS50"}, "386414__D1VYE0__HMPREF9019_1663": {"clade": "s__Prevotella_timonensis", "ext": [], "len": 1065, "score": 0, "taxon": "k__Bacteria|p__Bacteroidetes|c__Bacteroidia|o__Bacteroidales|f__Prevotellaceae|g__Prevotella|s__Prevotella_timonensis"}, "29394__H3NGH1__B5772_02200": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 972, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NFY1__B8A41_07655": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 534, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NC06__B8A41_08715": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 1317, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NDE2__B8A45_00965": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 699, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NFL8__B8A45_06985": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 582, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NDD1__B8A45_02325": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 480, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NE37__B8A45_05170": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 756, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NCU9__B8A45_08665": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 765, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NFP2__B8A45_07105": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 651, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NEK0__HMPREF9703_00981": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 1110, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NFD8__HMPREF9703_01269": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 732, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NC21__HMPREF9703_00102": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 1617, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NCD6__B8A33_07975": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 1560, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NGF9__B8A33_01850": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 1086, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NET0__B8A33_05820": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 1332, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NDY2__B8A44_05015": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 3315, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NDH9__B8A44_07175": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 870, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NE98__B8A46_08570": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 1188, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NDD0__B8A46_01590": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 1173, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NCV0__B8A46_05195": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 888, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NE72__B8A39_01430": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 654, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NGD0__B8A39_02610": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 1620, "score": 0, "taxon": "k__Bacteria|p__Firmicutes|c__Bacilli|o__Lactobacillales|f__Carnobacteriaceae|g__Dolosigranulum|s__Dolosigranulum_pigrum"}, "29394__H3NCJ1__B8A42_01505": {"clade": "s__Dolosigranulum_pigrum", "ext": [], "len": 930, "score": 0, "taxon": 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