Mercurial > repos > iuc > meryl
comparison meryl.xml @ 11:29dabd8db6f2 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/meryl commit e479a44b3c6e7a3c0facdb325892b0a6da6f0cd2
author | iuc |
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date | Thu, 02 Jun 2022 17:17:45 +0000 |
parents | 9cd178127b19 |
children |
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10:9cd178127b19 | 11:29dabd8db6f2 |
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92 mkdir 'child${i}.meryl' && | 92 mkdir 'child${i}.meryl' && |
93 ln -s '$read' ./input_child_${i}.${read.ext} && | 93 ln -s '$read' ./input_child_${i}.${read.ext} && |
94 meryl count k=$size memory=\$GALAXY_MEMORY_GB threads=\${GALAXY_SLOTS:-1} ./input_child_${i}.${read.ext} output 'child${i}.meryl' && | 94 meryl count k=$size memory=\$GALAXY_MEMORY_GB threads=\${GALAXY_SLOTS:-1} ./input_child_${i}.${read.ext} output 'child${i}.meryl' && |
95 #end for | 95 #end for |
96 meryl union-sum child*.meryl output child.meryl && | 96 meryl union-sum child*.meryl output child.meryl && |
97 meryl histogram child.meryl > read-db.hist && | |
98 tar -czf 'read-db.meryldb' child.meryl && | |
99 | |
97 ## mat specific kmers | 100 ## mat specific kmers |
98 meryl difference mat.meryl pat.meryl output mat.only.meryl && | 101 meryl difference mat.meryl pat.meryl output mat.only.meryl && |
99 meryl histogram mat.only.meryl > mat.only.hist && | 102 meryl histogram mat.only.meryl > mat.only.hist && |
100 java -jar -Xmx1g \$MERQURY/eval/kmerHistToPloidyDepth.jar mat.only.hist > mat.only.ploidy && | 103 java -jar -Xmx1g \$MERQURY/eval/kmerHistToPloidyDepth.jar mat.only.hist > mat.only.ploidy && |
101 VAR=`sed -n 2p mat.only.ploidy | awk '{print \$NF}'` && | 104 VAR=`sed -n 2p mat.only.ploidy | awk '{print \$NF}'` && |
125 java -jar -Xmx1g \$MERQURY/eval/kmerHistToPloidyDepth.jar pat.inherited.hist > pat.inherited.ploidy && | 128 java -jar -Xmx1g \$MERQURY/eval/kmerHistToPloidyDepth.jar pat.inherited.hist > pat.inherited.ploidy && |
126 VAR=`sed -n 2p pat.inherited.ploidy | awk '{print \$NF}'` && | 129 VAR=`sed -n 2p pat.inherited.ploidy | awk '{print \$NF}'` && |
127 meryl greater-than \$VAR output pat.hapmer.meryl pat.inherited.meryl && | 130 meryl greater-than \$VAR output pat.hapmer.meryl pat.inherited.meryl && |
128 tar -czf 'pat.meryldb' pat.hapmer.meryl && | 131 tar -czf 'pat.meryldb' pat.hapmer.meryl && |
129 | 132 |
130 ## shared hapmers | |
131 meryl intersect output shared.inherited.meryl child.meryl shared.meryl && | |
132 meryl histogram shared.inherited.meryl > shared.inherited.hist && | |
133 java -jar -Xmx1g \$MERQURY/eval/kmerHistToPloidyDepth.jar shared.inherited.hist > shared.inherited.ploidy && | |
134 VAR=`sed -n 2p shared.inherited.ploidy | awk '{print \$NF}'` && | |
135 meryl greater-than \$VAR output shared.filt.meryl shared.inherited.meryl && | |
136 | |
137 ## child hapmers | |
138 meryl union-sum output child.inherited.meryl mat.inherited.meryl pat.inherited.meryl shared.inherited.meryl && | |
139 meryl difference output read.only.meryl child.meryl child.inherited.meryl && | |
140 tar -czf 'read-db.meryldb' read.only.meryl && | |
141 echo 'K-mer size: ${size}' | 133 echo 'K-mer size: ${size}' |
142 #end if | 134 #end if |
143 ]]> </command> | 135 ]]> </command> |
144 <inputs> | 136 <inputs> |
145 <conditional name="operation_type"> | 137 <conditional name="operation_type"> |
586 <param name="child_reads" value="child.fasta" /> | 578 <param name="child_reads" value="child.fasta" /> |
587 <param name="paternal_reads" value="paternal.fasta" /> | 579 <param name="paternal_reads" value="paternal.fasta" /> |
588 <param name="maternal_reads" value="maternal.fasta" /> | 580 <param name="maternal_reads" value="maternal.fasta" /> |
589 <conditional name="options_kmer_size"> | 581 <conditional name="options_kmer_size"> |
590 <param name="kmer_size" value="provide" /> | 582 <param name="kmer_size" value="provide" /> |
591 <param name="input_kmer_size" value="7" /> | 583 <param name="input_kmer_size" value="21" /> |
592 </conditional> | 584 </conditional> |
593 </conditional> | 585 </conditional> |
594 <output name="read_db" ftype="meryldb"> | 586 <output name="read_db" ftype="meryldb"> |
595 <assert_contents> | 587 <assert_contents> |
596 <has_size value="1573" delta="300" /> | 588 <has_size value="3362942" delta="300" /> |
597 </assert_contents> | 589 </assert_contents> |
598 </output> | 590 </output> |
599 <output name="read_db_hist" file="output_23.read-db.hist" /> | 591 <output name="read_db_hist" file="output_23.read-db.hist" /> |
600 <output name="pat_db" ftype="meryldb"> | 592 <output name="pat_db" ftype="meryldb"> |
601 <assert_contents> | 593 <assert_contents> |
602 <has_size value="1779" delta="300" /> | 594 <has_size value="120610" delta="300" /> |
603 </assert_contents> | 595 </assert_contents> |
604 </output> | 596 </output> |
605 <output name="pat_db_hist" file="output_23.pat.hist" /> | 597 <output name="pat_db_hist" file="output_23.pat.hist" /> |
606 <output name="mat_db" ftype="meryldb"> | 598 <output name="mat_db" ftype="meryldb"> |
607 <assert_contents> | 599 <assert_contents> |
608 <has_size value="1569" delta="300" /> | 600 <has_size value="67883" delta="300" /> |
609 </assert_contents> | 601 </assert_contents> |
610 </output> | 602 </output> |
611 <output name="mat_db_hist" file="output_23.mat.hist" /> | 603 <output name="mat_db_hist" file="output_23.mat.hist" /> |
612 </test> | 604 </test> |
613 <test expect_num_outputs="1"> | 605 <test expect_num_outputs="1"> |