comparison minimap2.xml @ 1:b103bc946f57 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/minimap2 commit 160b3085e5c9152fc8eaeb2e8afc12cdc1abe739
author iuc
date Wed, 08 Nov 2017 10:02:24 -0500
parents 2445d53549ba
children 4070f129540a
comparison
equal deleted inserted replaced
0:2445d53549ba 1:b103bc946f57
1 <?xml version="1.0"?> 1 <?xml version="1.0"?>
2 <tool id="minimap2" name="Map with minimap2" version="2.3" profile="17.01"> 2 <tool id="minimap2" name="Map with minimap2" version="2.4" profile="17.01">
3 <description>A fast pairwise aligner for genomic and spliced nucleotide sequences</description> 3 <description>A fast pairwise aligner for genomic and spliced nucleotide sequences</description>
4 <requirements> 4 <requirements>
5 <requirement type="package" version="2.3">minimap2</requirement> 5 <requirement type="package" version="2.4">minimap2</requirement>
6 <requirement type="package" version="1.6">samtools</requirement> 6 <requirement type="package" version="1.6">samtools</requirement>
7 </requirements> 7 </requirements>
8 <version_command>minimap2 --version</version_command> 8 <version_command>minimap2 --version</version_command>
9 <command> 9 <command>
10 <![CDATA[ 10 <![CDATA[
427 minimap2 may align ~1% of read bases with long CIGARs beyond the 427 minimap2 may align ~1% of read bases with long CIGARs beyond the
428 capability of BAM. If you convert such SAM/CRAM to BAM, Picard and 428 capability of BAM. If you convert such SAM/CRAM to BAM, Picard and
429 recent samtools will throw an error and abort. Older samtools and other 429 recent samtools will throw an error and abort. Older samtools and other
430 tools may create corrupted BAM. 430 tools may create corrupted BAM.
431 431
432 To avoid this issue, you can add option ``-L`` at the minimap2 command 432 To avoid this issue, you can add option ``-L`` at the minimap2 command line.
433 line. This option moves a long CIGAR to the ``CG`` tag and leaves a 433 This option moves a long CIGAR to the ``CG`` tag and leaves a fully clipped
434 fully clipped CIGAR at the SAM CIGAR column. Current tools that don’t 434 CIGAR at the SAM CIGAR column. Current tools that don’t read CIGAR
435 read CIGAR (e.g. merging and sorting) still work with such BAM records; 435 (e.g. merging and sorting) still work with such BAM records; tools that read
436 tools that read CIGAR will effectively ignore these records. I have pull 436 CIGAR will effectively ignore these records. It has been decided that future
437 requests to the SAM spec, htslib, htsjdk, bedtools2, Rsamtools and 437 tools will seamlessly recognize long-cigar records generated by option `-L`.
438 igv.js. If they are accepted, future versions of these tools will
439 seamlessly recognize long-cigar records generated by option ``-L``.
440 438
441 **TD;DR**: if you work with ultra-long reads and use tools that only 439 **TD;DR**: if you work with ultra-long reads and use tools that only
442 process BAM files, please add option ``-L``. 440 process BAM files, please add option ``-L``.
443 441
444 The cs optional tag 442 The cs optional tag