Mercurial > repos > iuc > minimap2
comparison minimap2.xml @ 1:b103bc946f57 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/minimap2 commit 160b3085e5c9152fc8eaeb2e8afc12cdc1abe739
author | iuc |
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date | Wed, 08 Nov 2017 10:02:24 -0500 |
parents | 2445d53549ba |
children | 4070f129540a |
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0:2445d53549ba | 1:b103bc946f57 |
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1 <?xml version="1.0"?> | 1 <?xml version="1.0"?> |
2 <tool id="minimap2" name="Map with minimap2" version="2.3" profile="17.01"> | 2 <tool id="minimap2" name="Map with minimap2" version="2.4" profile="17.01"> |
3 <description>A fast pairwise aligner for genomic and spliced nucleotide sequences</description> | 3 <description>A fast pairwise aligner for genomic and spliced nucleotide sequences</description> |
4 <requirements> | 4 <requirements> |
5 <requirement type="package" version="2.3">minimap2</requirement> | 5 <requirement type="package" version="2.4">minimap2</requirement> |
6 <requirement type="package" version="1.6">samtools</requirement> | 6 <requirement type="package" version="1.6">samtools</requirement> |
7 </requirements> | 7 </requirements> |
8 <version_command>minimap2 --version</version_command> | 8 <version_command>minimap2 --version</version_command> |
9 <command> | 9 <command> |
10 <![CDATA[ | 10 <![CDATA[ |
427 minimap2 may align ~1% of read bases with long CIGARs beyond the | 427 minimap2 may align ~1% of read bases with long CIGARs beyond the |
428 capability of BAM. If you convert such SAM/CRAM to BAM, Picard and | 428 capability of BAM. If you convert such SAM/CRAM to BAM, Picard and |
429 recent samtools will throw an error and abort. Older samtools and other | 429 recent samtools will throw an error and abort. Older samtools and other |
430 tools may create corrupted BAM. | 430 tools may create corrupted BAM. |
431 | 431 |
432 To avoid this issue, you can add option ``-L`` at the minimap2 command | 432 To avoid this issue, you can add option ``-L`` at the minimap2 command line. |
433 line. This option moves a long CIGAR to the ``CG`` tag and leaves a | 433 This option moves a long CIGAR to the ``CG`` tag and leaves a fully clipped |
434 fully clipped CIGAR at the SAM CIGAR column. Current tools that don’t | 434 CIGAR at the SAM CIGAR column. Current tools that don’t read CIGAR |
435 read CIGAR (e.g. merging and sorting) still work with such BAM records; | 435 (e.g. merging and sorting) still work with such BAM records; tools that read |
436 tools that read CIGAR will effectively ignore these records. I have pull | 436 CIGAR will effectively ignore these records. It has been decided that future |
437 requests to the SAM spec, htslib, htsjdk, bedtools2, Rsamtools and | 437 tools will seamlessly recognize long-cigar records generated by option `-L`. |
438 igv.js. If they are accepted, future versions of these tools will | |
439 seamlessly recognize long-cigar records generated by option ``-L``. | |
440 | 438 |
441 **TD;DR**: if you work with ultra-long reads and use tools that only | 439 **TD;DR**: if you work with ultra-long reads and use tools that only |
442 process BAM files, please add option ``-L``. | 440 process BAM files, please add option ``-L``. |
443 | 441 |
444 The cs optional tag | 442 The cs optional tag |