comparison rarefaction.single.xml @ 2:871f24df79d8 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mothur commit 3418f23b9768f5aafb86488f5ec1cb97530d4fb3

1:62bf570a8a7d

    </macros>
    <expand macro="requirements"/>
    <expand macro="stdio"/>
    <expand macro="version_command"/>
    <command><![CDATA[
        @SHELL_OPTIONS@

        ## create symlinks to input datasets
        ln -s "$otu" otu.dat &&

        echo 'rarefaction.single(
            #if $otu.is_of_type("mothur.rabund"):
                rabund=otu.dat,
            #elif $otu.is_of_type("mothur.sabund"):
                sabund=otu.dat,
            #elif $otu.is_of_type("mothur.shared"):
                shared=otu.dat,
            #elif $otu.is_of_type("mothur.list"):
                list=otu.dat,
            #end if
            #if $label:
                label=${ str($label).replace(",","-") },
            #end if
            calc=${ str($calc).replace(",","-") },
            abund=$abund,
            iters=$iters,
            freq=$freq,
            processors='\${GALAXY_SLOTS:-8}'
        )'
        | sed 's/ //g'  ## mothur trips over whitespace
        | mothur
        | tee mothur.out.log

        ## rename output from sobs calculator to be consistent with others
        #if 'sobs' in str($calc).split(','):
            && mv otu\.*rarefaction otu.r_sobs
        #end if
    ]]></command>
    <inputs>
        <param name="otu" type="data" format="mothur.list,mothur.rabund,mothur.sabund,mothur.shared" label="list,rabund,sabund,shared - OTU List"/>
        <param name="label" type="select" label="label - OTU Labels" multiple="true">
            <expand macro="labeloptions"/>

            <option value="nseqs">nseqs - Utility the number of sequences in a sample</option>
        </param>
        <param name="abund" type="integer" value="10" min="0" label="abund - ACE Estimator threshold for abundant versus rare OTUs"/>
        <param name="iters" type="integer" value="1000" min="0" label="iters - Number of randomizations"/>
        <param name="freq" type="float" value="100" min="0" max="100" label="freq - Reporting frequency" help="if between 0 and 1 the fraction of sequences to sample, if greater than one - report every n iterations"/>
    </inputs>
    <outputs>
        <expand macro="logfile-output"/>
        <collection name="rarefactioncurves" type="list" label="${tool.name} on ${on_string}: rarefaction curves">
            <discover_datasets pattern=".*?(\.groups)?\.r_(?P&lt;designation&gt;.*)" format="tabular"/>

        <test><!-- test with default values -->
            <param name="otu" value="amazon.an.shared" ftype="mothur.shared"/>
            <output_collection name="rarefactioncurves" count="1">
                <element name="sobs" md5="c4c1f1970edf87ea5103e0704130f079" ftype="tabular"/>
            </output_collection>
            <expand macro="logfile-test"/>
        </test>
        <test><!-- test with all calculators and subset of labels -->
            <param name="otu" value="amazon.an.shared" ftype="mothur.shared"/>
            <param name="calc" value="ace,bootstrap,chao,jack,sobs,simpsoneven,shannoneven,heip,smithwilson,coverage,simpson,invsimpson,shannon,npshannon,nseqs"/>
            <param name="label" value="0.03,0.05,0.36,0.55"/>
            <output_collection name="rarefactioncurves" count="15">
                <element name="simpson" md5="8d6b7ad9a68c30c882e93b17d343042a" ftype="tabular"/>
            </output_collection>
            <expand macro="logfile-test"/>
        </test>
    </tests>
    <help>
<![CDATA[

@MOTHUR_OVERVIEW@

**Command Documentation**

The rarefaction.single_ command generates intra-sample rarefaction curves using a re-sampling without replacement approach. Rarefaction curves provide a way of comparing the richness observed in different samples.  For calc parameter choices see: https://www.mothur.org/wiki/Calculators

.. _rarefaction.single: https://www.mothur.org/wiki/Rarefaction.single
]]>
    </help>
    <expand macro="citations"/>
</tool>

2:871f24df79d8

    </macros>
    <expand macro="requirements"/>
    <expand macro="stdio"/>
    <expand macro="version_command"/>
    <command><![CDATA[
@SHELL_OPTIONS@

## create symlinks to input datasets
ln -s '$otu' otu.dat &&

echo 'rarefaction.single(
    #if $otu.is_of_type("mothur.rabund"):
        rabund=otu.dat,
    #elif $otu.is_of_type("mothur.sabund"):
        sabund=otu.dat,
    #elif $otu.is_of_type("mothur.shared"):
        shared=otu.dat,
    #elif $otu.is_of_type("mothur.list"):
        list=otu.dat,
    #end if
    #if $label:
        label=${ str($label).replace(",","-") },
    #end if
    calc=${ str($calc).replace(",","-") },
    abund=$abund,
    iters=$iters,
    freq=$freq,
    processors='\${GALAXY_SLOTS:-8}'
)'
| sed 's/ //g'  ## mothur trips over whitespace
| mothur
| tee mothur.out.log

## rename output from sobs calculator to be consistent with others
#if 'sobs' in str($calc).split(','):
    && mv otu\.*rarefaction otu.r_sobs
#end if
    ]]></command>
    <inputs>
        <param name="otu" type="data" format="mothur.list,mothur.rabund,mothur.sabund,mothur.shared" label="list,rabund,sabund,shared - OTU List"/>
        <param name="label" type="select" label="label - OTU Labels" multiple="true">
            <expand macro="labeloptions"/>

            <option value="nseqs">nseqs - Utility the number of sequences in a sample</option>
        </param>
        <param name="abund" type="integer" value="10" min="0" label="abund - ACE Estimator threshold for abundant versus rare OTUs"/>
        <param name="iters" type="integer" value="1000" min="0" label="iters - Number of randomizations"/>
        <param name="freq" type="float" value="100" min="0" max="100" label="freq - Reporting frequency" help="if between 0 and 1 the fraction of sequences to sample, if greater than one - report every n iterations"/>
        <expand macro="param-savelog"/>
    </inputs>
    <outputs>
        <expand macro="logfile-output"/>
        <collection name="rarefactioncurves" type="list" label="${tool.name} on ${on_string}: rarefaction curves">
            <discover_datasets pattern=".*?(\.groups)?\.r_(?P&lt;designation&gt;.*)" format="tabular"/>

        <test><!-- test with default values -->
            <param name="otu" value="amazon.an.shared" ftype="mothur.shared"/>
            <output_collection name="rarefactioncurves" count="1">
                <element name="sobs" md5="c4c1f1970edf87ea5103e0704130f079" ftype="tabular"/>
            </output_collection>
            <param name="savelog" value="true"/>
            <expand macro="logfile-test"/>
        </test>
        <test><!-- test with all calculators and subset of labels -->
            <param name="otu" value="amazon.an.shared" ftype="mothur.shared"/>
            <param name="calc" value="ace,bootstrap,chao,jack,sobs,simpsoneven,shannoneven,heip,smithwilson,coverage,simpson,invsimpson,shannon,npshannon,nseqs"/>
            <param name="label" value="0.03,0.05,0.36,0.55"/>
            <output_collection name="rarefactioncurves" count="15">
                <element name="simpson" md5="8d6b7ad9a68c30c882e93b17d343042a" ftype="tabular"/>
            </output_collection>
            <param name="savelog" value="true"/>
            <expand macro="logfile-test"/>
        </test>
    </tests>
    <help><![CDATA[

@MOTHUR_OVERVIEW@

**Command Documentation**

The rarefaction.single_ command generates intra-sample rarefaction curves using a re-sampling without replacement approach. Rarefaction curves provide a way of comparing the richness observed in different samples.  For calc parameter choices see: https://www.mothur.org/wiki/Calculators

.. _rarefaction.single: https://www.mothur.org/wiki/Rarefaction.single

    ]]></help>
    <expand macro="citations"/>
</tool>