Mercurial > repos > iuc > mykrobe_genotype
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/mykrobe commit 65f9e423263afc8f23fa51cb4770998b99f933ba"
author | iuc |
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date | Thu, 02 Jan 2020 12:04:06 -0500 |
parents | 84f769706f7a |
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<?xml version="1.1"?> <macros> <xml name="requirements"> <requirements> <requirement type="package" version="0.7.0">mykrobe</requirement> </requirements> </xml> <token name="@select_inputs@"> <![CDATA[ #set $name='sample' #set $type='fastq' ## Adding sample name, indicate specie and inputs fastq(s) files #if $data_type.type == "paired": ln -s '$data_type.fastq_input1' sample_1.fastq && ln -s '$data_type.fastq_input2' sample_2.fastq && #elif $data_type.type == "collection": #set $name=str($data_type.fastq_input1.name) ln -s '$data_type.fastq_input1.forward' '$data_type.fastq_input1.name'_1.fastq && ln -s '$data_type.fastq_input1.reverse' '$data_type.fastq_input1.name'_2.fastq && #elif $data_type.type == "single": #if $data_type.fastq_input1.is_of_type('fastqsanger') or $data_type.fastq_input1.is_of_type('fastq'): ln -s '$data_type.fastq_input1' sample.fastq && #end if #if $data_type.fastq_input1.is_of_type('bam'): ln -s '$data_type.fastq_input1' sample.bam && #set $type='bam' #end if #end if ]]> </token> <token name="@shared_options@"> <![CDATA[ #if $kmer: --kmer '${kmer}' #end if #if $expected_depth: --expected_depth '${expected_depth}' #end if $ont $report_all_calls #if $expected_error_rate: --expected_error_rate '${expected_error_rate}' #end if #if $min_variant_conf: --min_variant_conf '${min_variant_conf}' #end if #if $min_gene_conf: --min_gene_conf '${min_gene_conf}' #end if #if $min_proportion_expected_depth: --min_proportion_expected_depth '${min_proportion_expected_depth}' #end if #if $min_gene_percent_covg_threshold: --min_gene_percent_covg_threshold '${min_gene_percent_covg_threshold}' #end if $guess_sequence_method $ignore_minor_calls #if $ignore_filtered: --ignore_filtered '${ignore_filtered}' #end if #if $model: --model '${model}' #end if #if $ploidy: --ploidy '${ploidy}' #end if ]]> </token> <xml name="inputs"> <conditional name="data_type"> <param name="type" type="select" label="Specify the read type."> <option value="single">Single-end Data</option> <option value="paired">Paired-end Data</option> <option value="collection">Collection Paired-end Data</option> </param> <when value="single"> <param name="fastq_input1" type="data" format="fastqsanger, fastq,fasta,bam" label="Single end read file(s)"/> </when> <when value="paired"> <param name="fastq_input1" type="data" format="fastqsanger, fastq" label="Forward paired-end read file"/> <param name="fastq_input2" type="data" format="fastqsanger, fastq" label="Reverse paired-end read file"/> </when> <when value="collection"> <param name="fastq_input1" type="data_collection" label="Paired-end reads collection" optional="false" format="fastqsanger, fastq" collection_type="paired" /> </when> </conditional> </xml> <xml name="options"> <param argument="--kmer" optional="True" type="integer" min="0" label="Kmer length" value="21" help="default = 21"/> <param argument="--expected_error_rate" optional="True" type="float" label="Expected error rate" help="Expected sequencing error rate. Set to 0.15 for ONT genotyping"/> <param argument="--expected_depth" optional="True" type="integer" min="0" label="Expected depth" help=""/> <param argument="--ont" type="boolean" truevalue="--ont" falsevalue="" label="Set default for ONT data" help=""/> <param argument="--min_variant_conf" optional="True" type="integer" min="0" label="Minimum genotype confidence for variant genotyping" help=""/> <param argument="--min_gene_conf" optional="True" type="integer" min="0" label="Minimum genotype confidence for gene genotyping" help=""/> <param argument="--min_gene_percent_covg_threshold" optional="True" type="integer" min="0" label="Minimum gene predict coverage" help="all genes alleles found above this percent coverage will be reported (default 100 (only best alleles reported))"/> <param argument="--min_proportion_expected_depth" optional="True" type="float" label="Minimum depth required on the sum of both alleles" help="Default 0.3 (30%)"/> <param argument="--model" optional="True" type="data" format="txt" label="Genotype model used" help="default kmer_count. Options kmer_count, median_depth"/> <param argument="--ploidy" optional="True" type="select" label="Diploid or Haploid" help="Use a diploid (includes 0/1 calls) or haploid genotyping model"> <option value="diploid">diploid</option> <option value="haploid">haploid</option> </param> <param argument="--report_all_calls" type="boolean" truevalue="--report_all_calls" falsevalue="" label="Report all calls" help=""/> <param argument="--ignore_minor_calls" type="boolean" truevalue="--ignore_minor_calls" falsevalue="" label="Ignore minor calls" help="Ignore minor calls when running resistance prediction"/> <param argument="--ignore_filtered" type="data" format="txt" label="Ignore filtered" optional="True" help="Don't include filtered genotypes"/> <param argument="--guess_sequence_method" type="boolean" truevalue="--guess_sequence_method" falsevalue="" label="Guess sequence method" help="Guess if ONT or Illumia based on error rate. If error rate is > 10%, ploidy is set to haploid and a confidence threshold is used"/> </xml> <token name="@ATTRIBUTION@"> <![CDATA[ **MyKrobe predict - Antibiotic resistance predictions** Rapid antibiotic-resistance predictions from genome sequence data for Staphylococcus aureus and Mycobacterium tuberculosis using Bruijn graph. ]]> </token> <xml name="citation"> <citations> <citation type="doi">10.1038/ncomms10063</citation> </citations> </xml> </macros>