diff NanocomporeDB_process.py @ 0:b6eeea872fd2 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/nanocompore commit 652a872061324ba1073bfa286777ffeefa352671"

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/NanocomporeDB_process.py	Mon Jun 08 14:41:41 2020 -0400
@@ -0,0 +1,60 @@
+#!/usr/bin/env python
+
+import argparse
+import os
+
+from nanocompore.SampCompDB import SampCompDB
+
+
+def is_valid_file(file_name):
+    if os.path.isfile(file_name):
+        return os.path.abspath(file_name)
+    else:
+        raise FileNotFoundError(os.path.abspath(file_name))
+
+
+def is_valid_directory(dir_name):
+    if os.path.isdir(dir_name):
+        return os.path.abspath(dir_name)
+    else:
+        raise NotADirectoryError(os.path.abspath(dir_name))
+
+
+if __name__ == '__main__':
+
+    parser = argparse.ArgumentParser(
+        description='save nanocompre sampcomp \
+            results as interval outputs \
+            \nSample call: \"python Nannocompore-plot.py --db-path \
+            ./out_SampComp.db --ref-fasta ref.fa --annotation-bed annot.bed \
+            --out-dir ./plots/')
+
+    parser.add_argument('--ref-fasta', required=True, type=is_valid_file,
+                        help='The reference genome  used for read alignment.')
+    parser.add_argument('--db-path', default="./out_SampComp.db", type=str,
+                        help='Path to the SampCompDB database path prefix.')
+    parser.add_argument('--annotation-bed', required=False, type=is_valid_file,
+                        help='BED file containing the annotation of the transcriptome used as reference when mapping')
+    parser.add_argument('--pvalue-types', type=str,
+                        default='GMM_logit_pvalue,KS_dwell_pvalue,KS_intensity_pvalue',
+                        help='path to the annotations')
+    parser.add_argument('--bedgraph', default=False,
+                        help='write output in BEDGRAPH format instead of BED')
+    parser.add_argument('--pvalue-threshold', default=1.0,
+                        help='Maximum reported p-value.')
+    parser.add_argument('--out-dir', default="./", type=is_valid_directory,
+                        help='path the plotting output directory.')
+
+    args = parser.parse_args()
+
+    db = SampCompDB(args.db_path, fasta_fn=args.ref_fasta,
+                    bed_fn=args.annotation_bed)
+    print(db)
+    print("DB read ids:", db.ref_id_list)
+
+    if args.annotation_bed:
+        for pt in args.pvalue_types.split(','):
+            print("bedgraph output for p-value type:", pt)
+            db.save_to_bed(output_fn='{}/{}.bedgraph'.format(args.out_dir, pt),
+                           pvalue_field=pt, pvalue_thr=args.pvalue_threshold,
+                           bedgraph=args.bedgraph)