view ncbi_acc_download.xml @ 0:1c58de56d587 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ncbi_acc_download commit 6747338e8e02cb87c4f3b9cdea0b761f236a02d1"

<tool id="ncbi_acc_download" name="NCBI Accession Download" version="@TOOL_VERSION@+galaxy0">
    <description>Download sequences from GenBank/RefSeq by accession through the NCBI ENTREZ API</description>
    <macros>
        <import>macros.xml</import>
    </macros>
    <requirements>
        <requirement type="package" version="@TOOL_VERSION@">ncbi-acc-download</requirement>
    </requirements>
    <command detect_errors="exit_code"><![CDATA[
        #if $query_source.select == "accession_file":
            { grep -v "^[ \t]*$" $query_source.accession_file > accessions ||
            { echo "No accession numbers in input. Aborting." 1>&2; exit 1; } } &&
        #else if $query_source.select == "accession_list":
            echo '$query_source.accession_list' | sed -r 's/(\,|__cn__)/\n/g' | grep -v "^[ \t]*$" > accessions &&
        #end if
        mkdir outdir &&
        cd outdir &&
        ignore_errors=$ignore_failed &&
        while read accession; do
        echo "Downloading accession number: " \$accession " ..." >> ../error.log &&
        ncbi-acc-download
            --molecule '${molecule.select}'
            --format '${molecule.format}'
            #if $molecule.format != 'featuretable' and $molecule.format != 'gff3':
                --extended-validation all
            #end if
            \${accession};
        failure=\$?;
        if [ \$failure -ne 0 ]; then
            echo " failed." >> ../error.log;
            if [ \$ignore_errors -ne 0 ]; then
                echo \$accession >> ../failed.txt;
            else
                exit 1;
            fi;
        else
            echo " done." >> ../error.log;
        fi;
        sleep 2;
        done < ../accessions 2> >(tee -a ../error.log >&2);
    ]]></command>
    <inputs>
        <conditional name="query_source">
            <param name="select" type="select" label="Select source for IDs">
                <option value="accession_file">File containing Accessions (one per line)</option>
                <option value="accession_list">Direct Entry</option>
            </param>
            <when value="accession_file">
                <param label="Accession File" name="accession_file" type="data" format="txt,tabular"/>
            </when>
            <when value="accession_list">
                <param label="ID List" name="accession_list" type="text" area="true" help="Newline/Comma separated list of IDs">
                    <validator type="expression" message="ID list cannot be empty">value.strip()</validator>
                </param>
            </when>
        </conditional>
        <conditional name="molecule">
            <param name="select" type="select" label="Molecule Type">
                <option value="nucleotide" selected="true">Nucleotide</option>
                <option value="protein">Protein</option>
            </param>
            <when value="nucleotide">
                <param name="format" type="select" label="File Format">
                    <option value="fasta" selected="true">FASTA</option>
                    <option value="genbank">GenBank</option>
                    <option value="featuretable">Feature Table</option>
                    <option value="gff3">GFF3</option>
                </param>
            </when>
            <when value="protein">
                <param name="format" type="select" label="File Format">
                    <option value="fasta" selected="true">FASTA</option>
                </param>
            </when>
        </conditional>
        <param name="ignore_failed" type="select" display="radio"
        label="How to handle download failures">
            <option value="0">Abort with error on first failure</option>
            <option value="1">Add accession to failed list and continue</option>
        </param>
    </inputs>
    <outputs>
        <collection name="output" type="list" label="${tool.name} on ${on_string}: Downloaded Files">
            <discover_datasets pattern="(?P&lt;name&gt;.+)\.fa$" directory="outdir" format="fasta"/>
            <discover_datasets pattern="(?P&lt;name&gt;.+)\.gbk$" directory="outdir" format="genbank"/>
            <discover_datasets pattern="(?P&lt;name&gt;.+)\.gff$" directory="outdir" format="gff"/>
            <discover_datasets pattern="(?P&lt;name&gt;.+)\.ft$" directory="outdir" format="txt"/>
        </collection>
        <data name="error_log" from_work_dir="error.log" label="${tool.name} on ${on_string}: Log" format="txt"/>
        <data name="failed_accessions" from_work_dir="failed.txt" label="${tool.name} on ${on_string}: Failed accessions" format="txt">
            <filter>str(ignore_failed)=='1'</filter>
        </data>
    </outputs>
    <tests>
        <test>
            <conditional name="molecule">
                <param name="select" value="nucleotide"/>
                <param name="format" value="fasta"/>
            </conditional>
            <conditional name="query_source">
                <param name="select" value="accession_file" />
                <param name="accession_file" value="accessions_1.tsv"/>
            </conditional>
            <output_collection name="output" type="list">
                <element name="CP011064" ftype="fasta">
                    <assert_contents>
                        <has_line line=">CP011064.1 Escherichia coli str. Sanji plasmid pSJ_94, complete sequence" />
                    </assert_contents>
                </element>
                <element name="CP021680" ftype="fasta">
                    <assert_contents>
                        <has_line line=">CP021680.1 Escherichia coli strain AR_0162 plasmid tig00002623, complete sequence" />
                    </assert_contents>
                </element>
            </output_collection>
        </test>
        <test>
            <conditional name="molecule">
                <param name="select" value="nucleotide"/>
                <param name="format" value="genbank"/>
            </conditional>
            <conditional name="query_source">
                <param name="select" value="accession_file" />
                <param name="accession_file" value="accessions_1.tsv"/>
            </conditional>
            <output_collection name="output" type="list">
                <element name="CP011064" ftype="genbank">
                    <assert_contents>
                        <has_line line="DEFINITION  Escherichia coli str. Sanji plasmid pSJ_94, complete sequence." />
                    </assert_contents>
                </element>
                <element name="CP021680" ftype="genbank">
                    <assert_contents>
                        <has_line line="DEFINITION  Escherichia coli strain AR_0162 plasmid tig00002623, complete" />
                    </assert_contents>
                </element>
            </output_collection>
        </test>
        <test>
            <conditional name="molecule">
                <param name="select" value="nucleotide"/>
                <param name="format" value="gff3"/>
            </conditional>
            <conditional name="query_source">
                <param name="select" value="accession_file" />
                <param name="accession_file" value="accessions_1.tsv"/>
            </conditional>
            <output_collection name="output" type="list">
                <element name="CP011064" ftype="gff">
                    <assert_contents>
                        <has_line line="##sequence-region CP011064.1 1 94712" />
                    </assert_contents>
                </element>
                <element name="CP021680" ftype="gff">
                    <assert_contents>
                        <has_line line="##sequence-region CP021680.1 1 23332" />
                    </assert_contents>
                </element>
            </output_collection>
        </test>
        <test>
            <conditional name="molecule">
                <param name="select" value="nucleotide"/>
                <param name="format" value="featuretable"/>
            </conditional>
            <conditional name="query_source">
                <param name="select" value="accession_file" />
                <param name="accession_file" value="accessions_1.tsv"/>
            </conditional>
            <output_collection name="output" type="list">
                <element name="CP011064" ftype="txt">
                    <assert_contents>
                        <has_line line=">Feature gb|CP011064.1|" />
                    </assert_contents>
                </element>
                <element name="CP021680" ftype="txt">
                    <assert_contents>
                        <has_line line=">Feature gb|CP021680.1|" />
                    </assert_contents>
                </element>
            </output_collection>
        </test>
        <test>
            <conditional name="molecule">
                <param name="select" value="nucleotide"/>
                <param name="format" value="fasta"/>
            </conditional>
            <conditional name="query_source">
                <param name="select" value="accession_list" />
                <param name="accession_list" value="CP011064,CP021680"/>
            </conditional>
            <output_collection name="output" type="list">
                <element name="CP011064" ftype="fasta">
                    <assert_contents>
                        <has_line line=">CP011064.1 Escherichia coli str. Sanji plasmid pSJ_94, complete sequence" />
                    </assert_contents>
                </element>
                <element name="CP021680" ftype="fasta">
                    <assert_contents>
                        <has_line line=">CP021680.1 Escherichia coli strain AR_0162 plasmid tig00002623, complete sequence" />
                    </assert_contents>
                </element>
            </output_collection>
        </test>
        <test>
            <conditional name="molecule">
                <param name="select" value="nucleotide"/>
                <param name="format" value="fasta"/>
            </conditional>
            <conditional name="query_source">
                <param name="select" value="accession_list" />
                <param name="accession_list" value="CP011064,CP0XXXXX,CP021680"/>
            </conditional>
            <param name="ignore_failed" value="1" />
            <output_collection name="output" type="list">
                <element name="CP011064" ftype="fasta">
                    <assert_contents>
                        <has_line line=">CP011064.1 Escherichia coli str. Sanji plasmid pSJ_94, complete sequence" />
                    </assert_contents>
                </element>
                <element name="CP021680" ftype="fasta">
                    <assert_contents>
                        <has_line line=">CP021680.1 Escherichia coli strain AR_0162 plasmid tig00002623, complete sequence" />
                    </assert_contents>
                </element>
            </output_collection>
            <output name="failed_accessions">
                <assert_contents>
                    <has_line line="CP0XXXXX" />
                </assert_contents>
            </output>
        </test>
        <test>
            <conditional name="molecule">
                <param name="select" value="nucleotide"/>
                <param name="format" value="fasta"/>
            </conditional>
            <conditional name="query_source">
                <param name="select" value="accession_list" />
                <param name="accession_list" value="CP0XXXXX"/>
            </conditional>
            <param name="ignore_failed" value="1" />
            <output name="failed_accessions">
                <assert_contents>
                    <has_line line="CP0XXXXX" />
                </assert_contents>
            </output>
        </test>
        <test expect_failure="true">
            <conditional name="molecule">
                <param name="select" value="nucleotide"/>
                <param name="format" value="fasta"/>
            </conditional>
            <conditional name="query_source">
                <param name="select" value="accession_list" />
                <param name="accession_list" value="CP011064,CP0XXXXX,CP021680"/>
            </conditional>
            <param name="ignore_failed" value="0" />
        </test>
        <test>
            <conditional name="molecule">
                <param name="select" value="nucleotide"/>
                <param name="format" value="fasta"/>
            </conditional>
            <conditional name="query_source">
                <param name="select" value="accession_list" />
                <param name="accession_list" value="CP011064&#10;CP021680"/>
            </conditional>
            <output_collection name="output" type="list">
                <element name="CP011064" ftype="fasta">
                    <assert_contents>
                        <has_line line=">CP011064.1 Escherichia coli str. Sanji plasmid pSJ_94, complete sequence" />
                    </assert_contents>
                </element>
                <element name="CP021680" ftype="fasta">
                    <assert_contents>
                        <has_line line=">CP021680.1 Escherichia coli strain AR_0162 plasmid tig00002623, complete sequence" />
                    </assert_contents>
                </element>
            </output_collection>
        </test>
        <test>
            <conditional name="molecule">
                <param name="select" value="protein"/>
                <param name="format" value="fasta"/>
            </conditional>
            <conditional name="query_source">
                <param name="select" value="accession_list" />
                <param name="accession_list" value="NP_003192"/>
            </conditional>
            <output_collection name="output" type="list">
                <element name="NP_003192" ftype="fasta">
                    <assert_contents>
                        <has_line line=">NP_003192.1 transcription factor A, mitochondrial isoform 1 precursor [Homo sapiens]" />
                    </assert_contents>
                </element>
            </output_collection>
        </test>
    </tests>
    <help><![CDATA[
**What it does**
Given a file containing a list of NCBI accession numbers or a direct entry of accession numbers in the tool text input box, this tool will download the corresponding sequence records via the NCBI API. 

**Limitations**
- For protein sequence downloads, only fasta format is supported
- To avoid rate-limits imposed by the NCBI API, records are downloaded sequentially with a delay between requests. This may make it impractical to use this tool to download many (>100) records.

**Output**
A collection of sequence records in the desired format.
    ]]></help>
    <citations>
    </citations>
</tool>