# HG changeset patch # User iuc # Date 1705097499 0 # Node ID 3cdb96f2855dd88578ac77efd66ec44aa6893ab4 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/ncbi_fcs_gx commit 4a6561ed00e004260be3f3c29d81e814c60e20af diff -r 000000000000 -r 3cdb96f2855d macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,22 @@ + + + + ncbi-fcs-gx + + + + 0.5.0 + 0 + 21.05 + + + operation_3187 + + + + + 10.1101/2023.06.02.543519 + + + + diff -r 000000000000 -r 3cdb96f2855d ncbi_fcs_gx.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/ncbi_fcs_gx.xml Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,167 @@ + + detects contamination from foreign organisms in genome sequences + + macros.xml + + + + /dev/null 2>&1 && + ## run gx + run_gx.py + #if $mode.config_tag.fields.phone_home == "1" + --phone-home-label '$mode.config_tag.fields.phone_home_label' + #end if + --fasta '$mode.fasta' + #if $mode.id.id_selector == "gx_div" + --tax-id '1' + --div '$mode.id.div' + #else + --tax-id '$mode.id.tax_id' + #end if + #if $mode.species != "" + --species '$mode.species' + #end if + --split-fasta '$mode.screen_adv.split_fasta' + #if $mode.screen_adv.div: + --div '$mode.screen_adv.div' + #end if + --gx-db '$mode.config_tag.fields.node_cache_dir' + --out-basename output + --action-report true + --generate-logfile false +#elif $mode.mode_selector == "clean" + ## run gx + gx clean-genome + --input '$mode.input' + --action-report '$mode.action_report' + --contam-fasta-out 'contam.fa' + --min-seq-len '$mode.min_seq_len' + --output 'clean.fa' +#end if + ]]> + + $getVar('mode.screen_adv.gx_align_exclude_taxa', '') + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+ + + ^\s*\d+\s*(,\s*\d+\s*)*$ + + + + + + + + + + + + + + + + + + + + + +
+
+ + + + + +
+
+ + + + mode['mode_selector'] == 'screen' + + + mode['mode_selector'] == 'screen' + + + + mode['mode_selector'] == 'clean' + + + mode['mode_selector'] == 'clean' + + + + + + + + + + + + + + + + + + + + + + + +
diff -r 000000000000 -r 3cdb96f2855d test-data/fcsgx_test.fa.gz Binary file test-data/fcsgx_test.fa.gz has changed diff -r 000000000000 -r 3cdb96f2855d test-data/ncbi_fcs_gx_config.tsv --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ncbi_fcs_gx_config.tsv Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,4 @@ +## NCBI FCS GX Tool Config +# +#tag description use_source_manifest phone_home phone_home_label node_cache_dir +test-only Testing GX database 1 0 /tmp/gxdb diff -r 000000000000 -r 3cdb96f2855d test-data/ncbi_fcs_gx_databases.loc --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ncbi_fcs_gx_databases.loc Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,2 @@ +#tag source_manifest local_manifest +test-only https://ncbi-fcs-gx.s3.amazonaws.com/gxdb/test-only/test-only.manifest diff -r 000000000000 -r 3cdb96f2855d test-data/ncbi_fcs_gx_divisions.tsv --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/ncbi_fcs_gx_divisions.tsv Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,3 @@ +#gx_div tag description +prok:CFB group bacteria test-only Bacteria - CFB group bacteria +unkn:unknown test-only Unknown / Unclassified diff -r 000000000000 -r 3cdb96f2855d test-data/output.clean.fa.gz Binary file test-data/output.clean.fa.gz has changed diff -r 000000000000 -r 3cdb96f2855d test-data/output.contam.fa.gz Binary file test-data/output.contam.fa.gz has changed diff -r 000000000000 -r 3cdb96f2855d test-data/output.fcs_gx_report.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output.fcs_gx_report.txt Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,7 @@ +##[["FCS genome report", 2, 1], {"git-rev": "+branch--", "run-date": "Wed Jan 10 08:58:52 2024", "db": {"build-date": "2022-11-03", "seqs": 3042, "Gbp": 0.28336}, "run-info": {"agg-cvg": 0.358706, "asserted-div": "anml:insects", "inferred-primary-divs": ["prok:CFB group bacteria"], "corrected-primary-divs": ["anml:insects"]}}] +#seq_id start_pos end_pos seq_len action div agg_cont_cov top_tax_name +JPZV02005859.1 1 705930 705930 REVIEW prok:CFB group bacteria 22 Bacteroides xylanisolvens +JPZV02009577.1 1 600722 600722 EXCLUDE prok:CFB group bacteria 52 Bacteroides caecimuris +JPZV02016362.1 1 432265 432265 EXCLUDE prok:CFB group bacteria 45 Bacteroides salyersiae +JPZV02031416.1 1 170387 170387 REVIEW prok:CFB group bacteria 18 Bacteroides sp. CBA7301 +JPZV02034235.1 1 131158 131158 REVIEW prok:CFB group bacteria 24 Bacteroides faecichinchillae diff -r 000000000000 -r 3cdb96f2855d test-data/output.taxonomy.rpt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/output.taxonomy.rpt Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,8 @@ +##[["GX taxonomy analysis report", 3, 1], {"git-rev": "+branch--", "run-date": "Wed Jan 10 08:58:52 2024", "db": {"build-date": "2022-11-03", "seqs": 3042, "Gbp": 0.28336}, "run-info": {"agg-cvg": 0.358706, "asserted-div": "anml:insects", "inferred-primary-divs": ["prok:CFB group bacteria"], "corrected-primary-divs": ["anml:insects"]}}] +#seq-id seq-len (xp,lc,co,n,xc)-len cvg-by-all sep1 tax-name-1 tax-id-1 div-1 cvg-by-div-1 cvg-by-tax-1 score-1 sep2 tax-id-2 div-2 cvg-by-div-2 cvg-by-tax-2 score-2 sep3 tax-id-3 div-3 cvg-by-div-3 cvg-by-tax-3 score-3 sep4 tax-id-4 div-4 cvg-by-div-4 cvg-by-tax-4 score-4 sep5 reserved result div div_pct_cvg +JPZV02005859.1 705930 0,1139,0,0,0 159470 | Bacteroides xylanisolvens 371601 prok:CFB group bacteria 159470 49227 393 | 1796613 prok:CFB group bacteria 159470 48051 387 | | | n/a low-coverage prok:CFB group bacteria 23 +JPZV02009577.1 600722 0,65,0,0,0 316464 | Bacteroides caecimuris 1796613 prok:CFB group bacteria 316464 137645 782 | 371601 prok:CFB group bacteria 316464 120328 757 | | | n/a contaminant(div) prok:CFB group bacteria 53 +JPZV02016362.1 432265 0,0,0,0,0 197253 | Bacteroides salyersiae 291644 prok:CFB group bacteria 197253 74527 520 | 246787 prok:CFB group bacteria 197253 74971 512 | | | n/a contaminant(div) prok:CFB group bacteria 46 +JPZV02031416.1 170387 0,60,0,0,0 32395 | Bacteroides sp. CBA7301 2715212 prok:CFB group bacteria 32395 13706 205 | 1297750 prok:CFB group bacteria 32395 12253 201 | | | n/a low-coverage prok:CFB group bacteria 19 +JPZV02034235.1 131158 0,0,0,0,0 33221 | Bacteroides faecichinchillae 871325 prok:CFB group bacteria 33221 17243 230 | 1121098 prok:CFB group bacteria 33221 15138 222 | | | n/a low-coverage prok:CFB group bacteria 25 +JPZV02046037.1 26753 0,0,0,0,0 2720 | Bacteroides graminisolvens DSM 19988 = JCM 15093 1121097 prok:CFB group bacteria 2720 912 45 | 28111 prok:CFB group bacteria 2720 821 45 | | | n/a low-coverage prok:CFB group bacteria 10 diff -r 000000000000 -r 3cdb96f2855d tool-data/ncbi_fcs_gx_config.tsv.sample --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool-data/ncbi_fcs_gx_config.tsv.sample Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,5 @@ +## NCBI FCS GX Tool Configuration +# +#tag description use_source_manifest phone_home phone_home_label node_cache_dir +#all Complete GX database 0 0 /tmp/gxdb +#test-only Testing GX database 0 1 usegalaxy.org /tmp/gxdb diff -r 000000000000 -r 3cdb96f2855d tool-data/ncbi_fcs_gx_databases.loc.sample --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool-data/ncbi_fcs_gx_databases.loc.sample Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,8 @@ +## NCBI FCS GX Databases +# +#tag manifest path +#r2022-01-24 https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/database/r2022-01-24/all.manifest /big/data/dir/ncbi_fcs_gx_databases/r2022-01-24/all.manifest +#r2022-07-08 https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/database/r2022-07-08/all.manifest /big/data/dir/ncbi_fcs_gx_databases/r2022-07-08/all.manifest +#r2023-01-24 https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/database/r2023-01-24/all.manifest /big/data/dir/ncbi_fcs_gx_databases/r2023-01-24/all.manifest +#latest https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/database/latest/all.manifest /big/data/dir/ncbi_fcs_gx_databases/latest/all.manifest +#test-only https://ftp.ncbi.nlm.nih.gov/genomes/TOOLS/FCS/database/test-only/test-only.manifest /big/data/dir/ncbi_fcs_gx_databases/test-only/test-only.manifest diff -r 000000000000 -r 3cdb96f2855d tool-data/ncbi_fcs_gx_divisions.tsv.sample --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool-data/ncbi_fcs_gx_divisions.tsv.sample Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,64 @@ +#gx_div tag description +#anml:amphibians latest Animals (Metazoa) - amphibians +#anml:basal metazoans latest Animals (Metazoa) - basal metazoans +#anml:birds latest Animals (Metazoa) - birds +#anml:brachiopods latest Animals (Metazoa) - brachiopods +#anml:crustaceans latest Animals (Metazoa) - crustaceans +#anml:echinoderms latest Animals (Metazoa) - echinoderms +#anml:fishes latest Animals (Metazoa) - fishes +#anml:insects latest Animals (Metazoa) - insects +#anml:mammals latest Animals (Metazoa) - mammals +#anml:marsupials latest Animals (Metazoa) - marsupials +#anml:molluscs latest Animals (Metazoa) - molluscs +#anml:nematodes latest Animals (Metazoa) - nematodes +#anml:primates latest Animals (Metazoa) - primates +#anml:reptiles latest Animals (Metazoa) - reptiles +#anml:rodents latest Animals (Metazoa) - rodents +#anml:rotifers latest Animals (Metazoa) - rotifers +#anml:tardigrades latest Animals (Metazoa) - tardigrades +#anml:worms latest Animals (Metazoa) - worms +#arch:archaea latest Archaea - archaea +#prok:CFB group bacteria latest Bacteria - CFB group bacteria +#prok:GNS bacteria latest Bacteria - GNS bacteria +#prok:a-proteobacteria latest Bacteria - a-proteobacteria +#prok:actinobacteria latest Bacteria - actinobacteria +#prok:aquificales latest Bacteria - aquificales +#prok:b-proteobacteria latest Bacteria - b-proteobacteria +#prok:bacteria latest Bacteria - bacteria +#prok:chlamydias latest Bacteria - chlamydias +#prok:cyanobacteria latest Bacteria - cyanobacteria +#prok:d-proteobacteria latest Bacteria - d-proteobacteria +#prok:firmicutes latest Bacteria - firmicutes +#prok:fusobacteria latest Bacteria - fusobacteria +#prok:g-proteobacteria latest Bacteria - g-proteobacteria +#prok:green sulfur bacteria latest Bacteria - green sulfur bacteria +#prok:high GC Gram+ latest Bacteria - high GC Gram+ +#prok:mycoplasmas latest Bacteria - mycoplasmas +#prok:planctomycetes latest Bacteria - planctomycetes +#prok:proteobacteria latest Bacteria - proteobacteria +#prok:spirochetes latest Bacteria - spirochetes +#prok:thermotogales latest Bacteria - thermotogales +#prok:verrucomicrobia latest Bacteria - verrucomicrobia +#fung:ascomycetes latest Fungi - ascomycetes +#fung:basidiomycetes latest Fungi - basidiomycetes +#fung:budding yeasts latest Fungi - budding yeasts +#fung:chytrids latest Fungi - chytrids +#fung:fungi latest Fungi - fungi +#fung:microsporidians latest Fungi - microsporidians +#plnt:green algae latest Plants (Viridiplantae) - green algae +#plnt:mosses latest Plants (Viridiplantae) - mosses +#plnt:plants latest Plants (Viridiplantae) - plants +#prst:algae latest Protists (other Eukaryota) - algae +#prst:alveolates latest Protists (other Eukaryota) - alveolates +#prst:cellular slime molds latest Protists (other Eukaryota) - cellular slime molds +#prst:cercozoans latest Protists (other Eukaryota) - cercozoans +#prst:choanoflagellates latest Protists (other Eukaryota) - choanoflagellates +#prst:euglenoids latest Protists (other Eukaryota) - euglenoids +#prst:monads latest Protists (other Eukaryota) - monads +#prst:protists latest Protists (other Eukaryota) - protists +#prst:slime nets latest Protists (other Eukaryota) - slime nets +#synt:synthetic latest Synthetic - synthetic +#unkn:unknown latest Unknown / Unclassified +#virs:eukaryotic viruses latest Virus - eukaryotic viruses +#virs:prokaryotic viruses latest Virus - prokaryotic viruses +#virs:viruses latest Virus - viruses diff -r 000000000000 -r 3cdb96f2855d tool_data_table_conf.xml.sample --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_data_table_conf.xml.sample Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,17 @@ + + + + value, name, use_source_manifest, phone_home, phone_home_label, node_cache_dir + +
+ + + value, source_manifest, name + +
+ + + value, tag, name + +
+
diff -r 000000000000 -r 3cdb96f2855d tool_data_table_conf.xml.test --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_data_table_conf.xml.test Fri Jan 12 22:11:39 2024 +0000 @@ -0,0 +1,17 @@ + + + + value, name, use_source_manifest, phone_home, phone_home_label, node_cache_dir + +
+ + + value, source_manifest, name + +
+ + + value, tag, name + +
+